MD09G1084000.v1.1
ERF Family

AP2 ERF and B3 domain-containing transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002443
Physical Location & Seq
Forward (+)
5991104 .. 5991410
307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1084000.v1.1.491

Sequence Viewer

Length: 237 bp
ATGGTTATACGAAAACAACATGCCGAGAATCATTTTCAGGTTCATTTGAGTGCAGGGTTTGGTAAAGGTGTCTTGTTGAAATTTGAGGATCAGGGGTGGATTCATTTTGTGAAGGAGAAGAAACTGAAAGTTGGTGATGTTGTGAGGTTTGAGAGATCGGTAGGGGAGAATAAGAACCTAGAAACCTCGACATTTCGGGGATCGGAGAGATGCATGGTAGGGTCGGTGACCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

79

Amino Acids

8.9

Weight (kDa)

9.58

Isoelectric Point (pI)

18.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 28 - 67 6.3e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 96, 208
AcsI RAATTY 1 cut(s) 80
AgsI TTSAA 1 cut(s) 79
AlwI GGATC 2 cut(s) 96, 208
ApoI RAATTY 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 146
BfaI CTAG 1 cut(s) 179
BmsI GCATC 1 cut(s) 200
BsgI GTGCAG 1 cut(s) 72
Bsp143I GATC 3 cut(s) 88, 155, 200
BspPI GGATC 2 cut(s) 96, 208
BssMI GATC 3 cut(s) 88, 155, 200
BstEII GGTNACC 1 cut(s) 226
BstKTI GATC 3 cut(s) 91, 158, 203
BstMBI GATC 3 cut(s) 88, 155, 200
BstNSI RCATGY 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 226
CviAII CATG 2 cut(s) 20, 214
DpnI GATC 3 cut(s) 90, 157, 202
DpnII GATC 3 cut(s) 88, 155, 200
Eco91I GGTNACC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 215
FaeI CATG 2 cut(s) 23, 217
FaiI YATR 3 cut(s) 8, 21, 215
FatI CATG 2 cut(s) 19, 213
FspBI CTAG 1 cut(s) 179
Hin1II CATG 2 cut(s) 23, 217
HinfI GANTC 2 cut(s) 28, 100
HphI GGTGA 1 cut(s) 146
Hpy188I TCNGA 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 106
HpyCH4V TGCA 2 cut(s) 53, 213
Hsp92II CATG 2 cut(s) 23, 217
Kzo9I GATC 3 cut(s) 88, 155, 200
LpnPI CCDG 3 cut(s) 23, 39, 77
LweI GCATC 1 cut(s) 200
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 1 cut(s) 226
MalI GATC 3 cut(s) 90, 157, 202
MboI GATC 3 cut(s) 88, 155, 200
MboII GAAGA 1 cut(s) 130
MluCI AATT 1 cut(s) 80
MnlI CCTC 3 cut(s) 79, 138, 196
Mph1103I ATGCAT 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 48
NdeII GATC 3 cut(s) 88, 155, 200
NlaIII CATG 2 cut(s) 23, 217
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 215
NspI RCATGY 1 cut(s) 23
PfeI GAWTC 2 cut(s) 28, 100
PspEI GGTNACC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 48
Sau3AI GATC 3 cut(s) 88, 155, 200
SetI ASST 5 cut(s) 42, 70, 149, 180, 188
SfaNI GCATC 1 cut(s) 200
SmiMI CAYNNNNRTG 1 cut(s) 48
Sse9I AATT 1 cut(s) 80
SspMI CTAG 1 cut(s) 179
TaqI TCGA 1 cut(s) 188
TasI AATT 1 cut(s) 80
TfiI GAWTC 2 cut(s) 28, 100
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 2 cut(s) 32, 92
XapI RAATTY 1 cut(s) 80
XceI RCATGY 1 cut(s) 23
XspI CTAG 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.