MD17G1073800.v1.1
ERF Family

AP2 ERF and B3 domain-containing transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002443
Physical Location & Seq
Forward (+)
5997263 .. 5998174
912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1073800.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGATACGATGAGTTGCTGGAATGCGAGCACAAGTGACTACTCAACAATGAATGGAGCAAACGGTGCACGTTCAAACAAGTTGCCTTCTTCAAGGTACAAAGGAGTAGTGCCACAGTCAAATGGCAGGTGGGGAGCTCAAATATACGACAAGAGTCATCGTGTATGGTTGGGAACCTTCAATGATGAAGAAGAAGCTGCTAAAACCTACAACATTGCCTTGCTAAAGTTCCGGGGTCTTGATGCCATCACACACCTCAGTCGAAGCCAAATGAAACCCTACGGACGTGATGTCACGGAGGCTCTTTTCTTGGAATCCCATTCCAAGGCTGAAATCATTGACATGCTTCGAAAACATTCTTATGCCAACGAGCTCGAGATGTACAAGCATAAGTTGCACAATGGTAGTGCTCTTGGCCTTGATGATGGTGGAAAGCGTACGAAGTGTTACCTTTATTTAATGGACACGTGTAGTCGCGAGAGGGAGTTGCTTTTCGAGAAAGTGGCAACACCAAGCGACGTAGGGAGATTGAACCGCATGGTTATACCAAAACAACATGCCGAGAAGCATTTTCAGGTTCATCTGAGTGTAGGGTTTGGTAAAGGGGTGTTGTTGAAATTTGAGGATGAGTTGGGGAAGGTGTGGAGGTTTGGGTACTGTTATTGGAGTAGTAGTCAGAGTTATGTGTTGAGCAAAGGGTGGATTCGTTTTGTGAAGGAGAAGAAGCTGAAAGCTGGTGATATTGTGATGTTTCAGAGATCGGTAGGGGAGGATAAGAAGCTGTTCATTGAGTGTAGACCTAGAAAAGTCGATGTTTCGCGGATCGAGGAGATGCATGGTAGGGTTGCTGGCCCTTTAGCGACGGTTCAAGATGATGGAGTGGTGAGGTTGTTCGGAGTTAACATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

304

Amino Acids

34.53

Weight (kDa)

9.32

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 165 - 267 4.3e-25 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 117
Acc36I ACCTGC 1 cut(s) 117
AccI GTMKAC 1 cut(s) 796
AccII CGCG 2 cut(s) 477, 820
AciI CCGC 2 cut(s) 535, 820
AclWI GGATC 1 cut(s) 830
AcsI RAATTY 1 cut(s) 617
AcvI CACGTG 1 cut(s) 468
AfaI GTAC 4 cut(s) 98, 383, 439, 656
AfiI CCNNNNNNNGG 1 cut(s) 325
AflIII ACRYGT 2 cut(s) 465, 467
AgsI TTSAA 6 cut(s) 75, 93, 181, 532, 616, 869
AhdI GACNNNNNGTC 1 cut(s) 290
AjiI CACGTC 1 cut(s) 287
AluBI AGCT 6 cut(s) 137, 197, 373, 727, 734, 781
AluI AGCT 6 cut(s) 137, 197, 373, 727, 734, 781
Alw21I GWGCWC 5 cut(s) 32, 70, 139, 375, 412
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 2 cut(s) 415, 850
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 1 cut(s) 617
Asp700I GAANNNNTTC 2 cut(s) 355, 782
AspS9I GGNCC 1 cut(s) 851
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 749, 895
AsuII TTCGAA 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 70
BanII GRGCYC 2 cut(s) 139, 375
BbrPI CACGTG 1 cut(s) 468
Bbv12I GWGCWC 5 cut(s) 32, 70, 139, 375, 412
BbvI GCAGC 1 cut(s) 184
BccI CCATC 3 cut(s) 254, 419, 869
BcnI CCSGG 1 cut(s) 233
BfaI CTAG 1 cut(s) 801
BfuAI ACCTGC 1 cut(s) 117
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 1 cut(s) 233
BmeRI GACNNNNNGTC 1 cut(s) 290
BmeT110I CYCGRG 1 cut(s) 374
BmgBI CACGTC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 851
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 2 cut(s) 232, 822
BoxI GACNNNNGTC 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 349
BpuMI CCSGG 1 cut(s) 233
BsaAI YACGTR 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 232, 324
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse3DI GCAATG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 232, 324
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 2 cut(s) 271, 575
BseRI GAGGAG 1 cut(s) 842
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 1 cut(s) 184
Bsh1236I CGCG 2 cut(s) 477, 820
BshFI GGCC 2 cut(s) 417, 852
BsiHKAI GWGCWC 5 cut(s) 32, 70, 139, 375, 412
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 1 cut(s) 232
BsiWI CGTACG 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 325
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 2 cut(s) 417, 852
BsoBI CYCGRG 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 5 cut(s) 32, 70, 139, 375, 412
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 2 cut(s) 758, 822
Bsp68I TCGCGA 1 cut(s) 477
BspACI CCGC 2 cut(s) 535, 820
BspANI GGCC 2 cut(s) 417, 852
BspCNI CTCAG 2 cut(s) 270, 576
BspFNI CGCG 2 cut(s) 477, 820
BspHI TCATGA 1 cut(s) 908
BspLI GGNNCC 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 117
BspPI GGATC 1 cut(s) 830
BspT104I TTCGAA 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 381
BssECI CCNNGG 2 cut(s) 232, 324
BssMI GATC 2 cut(s) 758, 822
BssT1I CCWWGG 1 cut(s) 324
Bst4CI ACNGT 4 cut(s) 65, 117, 659, 865
BstAPI GCANNNNNTGC 2 cut(s) 65, 394
BstAUI TGTACA 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 468
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 28, 850
BstDEI CTNAG 2 cut(s) 257, 584
BstF5I GGATG 1 cut(s) 631
BstFNI CGCG 2 cut(s) 477, 820
BstKTI GATC 2 cut(s) 761, 825
BstMBI GATC 2 cut(s) 758, 822
BstMWI GCNNNNNNNGC 2 cut(s) 65, 394
BstNSI RCATGY 2 cut(s) 346, 560
BstPAI GACNNNNGTC 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 231
BstSLI GKGCMC 1 cut(s) 70
BstUI CGCG 2 cut(s) 477, 820
BstV1I GCAGC 1 cut(s) 184
BsuRI GGCC 2 cut(s) 417, 852
BtrI CACGTC 1 cut(s) 287
BtsCI GGATG 1 cut(s) 631
BtuMI TCGCGA 1 cut(s) 477
BveI ACCTGC 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 28, 850
CciI TCATGA 1 cut(s) 908
Cfr13I GGNCC 1 cut(s) 851
Csp6I GTAC 4 cut(s) 97, 382, 438, 655
CviAII CATG 5 cut(s) 343, 538, 557, 836, 909
CviQI GTAC 4 cut(s) 97, 382, 438, 655
DdeI CTNAG 2 cut(s) 257, 584
DpnI GATC 2 cut(s) 760, 824
DpnII GATC 2 cut(s) 758, 822
DriI GACNNNNNGTC 1 cut(s) 290
Eam1105I GACNNNNNGTC 1 cut(s) 290
Ecl136II GAGCTC 2 cut(s) 137, 373
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 2 cut(s) 139, 375
Eco53kI GAGCTC 2 cut(s) 137, 373
Eco72I CACGTG 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 374
EcoICRI GAGCTC 2 cut(s) 137, 373
EcoT14I CCWWGG 1 cut(s) 324
EcoT22I ATGCAT 1 cut(s) 837
EcoT38I GRGCYC 2 cut(s) 139, 375
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 5 cut(s) 346, 541, 560, 839, 912
FatI CATG 5 cut(s) 342, 537, 556, 835, 908
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 1 cut(s) 638
FriOI GRGCYC 2 cut(s) 139, 375
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 1 cut(s) 801
GluI GCNGC 1 cut(s) 198
HaeIII GGCC 2 cut(s) 417, 852
HapII CCGG 1 cut(s) 232
Hin1II CATG 5 cut(s) 346, 541, 560, 839, 912
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HinfI GANTC 3 cut(s) 154, 314, 703
HpaI GTTAAC 1 cut(s) 901
HpaII CCGG 1 cut(s) 232
HphI GGTGA 2 cut(s) 749, 895
Hpy166II GTNNAC 3 cut(s) 68, 797, 901
Hpy188I TCNGA 4 cut(s) 585, 678, 756, 896
Hpy188III TCNNGA 6 cut(s) 239, 376, 476, 496, 869, 909
Hpy8I GTNNAC 3 cut(s) 68, 797, 901
Hpy99I CGWCG 2 cut(s) 521, 865
HpyAV CCTTC 4 cut(s) 96, 187, 631, 709
HpyCH4III ACNGT 4 cut(s) 65, 117, 659, 865
HpyCH4IV ACGT 4 cut(s) 70, 286, 467, 519
HpyCH4V TGCA 3 cut(s) 68, 397, 835
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 394
HpyF3I CTNAG 2 cut(s) 257, 584
HpySE526I ACGT 4 cut(s) 70, 286, 467, 519
Hsp92II CATG 5 cut(s) 346, 541, 560, 839, 912
KspAI GTTAAC 1 cut(s) 901
Kzo9I GATC 2 cut(s) 758, 822
LmnI GCTCC 2 cut(s) 56, 134
LpnPI CCDG 6 cut(s) 4, 112, 245, 560, 720, 834
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 2 cut(s) 232, 822
MaeI CTAG 1 cut(s) 801
MaeII ACGT 4 cut(s) 70, 286, 467, 519
MaeIII GTNAC 3 cut(s) 35, 292, 446
MalI GATC 2 cut(s) 760, 824
MboI GATC 2 cut(s) 758, 822
MboII GAAGA 4 cut(s) 81, 200, 203, 733
MhlI GDGCHC 5 cut(s) 32, 70, 139, 375, 412
MluCI AATT 1 cut(s) 617
MlyI GAGTC 1 cut(s) 163
MnlI CCTC 8 cut(s) 266, 292, 474, 616, 639, 763, 820, 879
Mph1103I ATGCAT 1 cut(s) 837
MroXI GAANNNNTTC 2 cut(s) 355, 782
MseI TTAA 2 cut(s) 458, 900
MslI CAYNNNNRTG 3 cut(s) 341, 360, 585
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 28
MvnI CGCG 2 cut(s) 477, 820
MwoI GCNNNNNNNGC 2 cut(s) 65, 394
NciI CCSGG 1 cut(s) 233
NdeII GATC 2 cut(s) 758, 822
NlaIII CATG 5 cut(s) 346, 541, 560, 839, 912
NlaIV GGNNCC 1 cut(s) 175
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 2 cut(s) 35, 292
NruI TCGCGA 1 cut(s) 477
NsiI ATGCAT 1 cut(s) 837
NspI RCATGY 2 cut(s) 346, 560
NspV TTCGAA 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 374
PagI TCATGA 1 cut(s) 908
PaqCI CACCTGC 1 cut(s) 117
PctI GAATGC 1 cut(s) 28
PdmI GAANNNNTTC 2 cut(s) 355, 782
PfeI GAWTC 2 cut(s) 314, 703
Pfl23II CGTACG 1 cut(s) 437
PkrI GCNGC 1 cut(s) 199
PleI GAGTC 1 cut(s) 162
PmaCI CACGTG 1 cut(s) 468
PmlI CACGTG 1 cut(s) 468
PpsI GAGTC 1 cut(s) 162
Ppu21I YACGTR 1 cut(s) 468
PshAI GACNNNNGTC 1 cut(s) 153
Psp124BI GAGCTC 2 cut(s) 139, 375
PspCI CACGTG 1 cut(s) 468
PspLI CGTACG 1 cut(s) 437
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 1 cut(s) 851
RruI TCGCGA 1 cut(s) 477
RsaI GTAC 4 cut(s) 98, 383, 439, 656
RsaNI GTAC 4 cut(s) 97, 382, 438, 655
RseI CAYNNNNRTG 3 cut(s) 341, 360, 585
SacI GAGCTC 2 cut(s) 139, 375
SaqAI TTAA 2 cut(s) 458, 900
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 2 cut(s) 758, 822
Sau96I GGNCC 1 cut(s) 851
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 5 cut(s) 32, 70, 139, 375, 412
SfaNI GCATC 2 cut(s) 232, 822
Sfr274I CTCGAG 1 cut(s) 374
SfuI TTCGAA 1 cut(s) 349
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 3 cut(s) 341, 360, 585
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 1 cut(s) 617
SsiI CCGC 2 cut(s) 535, 820
SspMI CTAG 1 cut(s) 801
SstI GAGCTC 2 cut(s) 139, 375
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 4 cut(s) 65, 117, 659, 865
TaiI ACGT 4 cut(s) 73, 289, 470, 522
TaqI TCGA 6 cut(s) 262, 349, 375, 495, 810, 825
TasI AATT 1 cut(s) 617
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 2 cut(s) 314, 703
Tru1I TTAA 2 cut(s) 458, 900
Tru9I TTAA 2 cut(s) 458, 900
TseFI GTSAC 2 cut(s) 35, 292
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 2 cut(s) 35, 292
TspDTI ATGAA 5 cut(s) 65, 201, 287, 569, 775
TspGWI ACGGA 2 cut(s) 297, 311
VneI GTGCAC 1 cut(s) 66
XapI RAATTY 1 cut(s) 617
XceI RCATGY 2 cut(s) 346, 560
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 2 cut(s) 355, 782
XspI CTAG 1 cut(s) 801
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.