Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002443

Physical Location & Seq

Genomic Coordinates Reverse (-)

3187035 .. 3188255

Gene Structure 1221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1046100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGGACGGAATAAGCAGCACAGAAGAGAGCACAAGCAGCGACTCCATATCCATTTCCCCACTCCAACATATTGTTGCACGTGTGGACCCATTTGCCAAGTCAGCACCCCAAGTGGCCAGCCTCTGCCGCGTCGGCAGCGGCGCCAGCAGTGTGATTCTCGACCCCGAGCTCAGCAGCAGCGGCGGCACTGGCGGCGTCGAGGCCGAGTCCCGGAAGCTCCCTTCCTCCAGGTACAAAGGCGTGGTCCCGCAGCCCAACGGACGCTGGGGTGCCCAGATTTACGAGAAGCACCAGCGCGTCTGGCTGGGTACCTTCAACGAAGAGGACGAGGCCGCCAGGGCCTACGACGTTGCTGCACAGCGCTTCCGTGGCCGCGACGCCGTCACCAACTTTAAGCCCTCATCCGCCGAACCCATTTCCTCCGACGACGAAGAAAACGACGACGCCGAGGCCGCGTTTCTGAGCTCCCACTCTAAGTCCGAAATCGTCGACATGCTGCGAAAGCACACTTACAATGACGAGCTGGAACAGAGCAAGCGCAACAACTCCGCTTACGGGAAGCGGTCCCGGTCCAACGGATCACTGGGCCTGTTCGGTACGGACAACAGTGGAGTCCCGAAAGCGCGTGAGCAGCTTTTCGAAAAAGCGGTGACCCCGAGCGATGTCGGGAAACTGAACCGCCTCGTGATCCCGAAGCAGCACGCGGAAAAGCACTTTCCTTTGCAAAGCGGAAGCGCTGCAACCTTAACGGTAAGCGCTTCTACCGCTTGCAAAGGAGTGCTTTTGAATTTTGAGGATGTCGGGGGCAAAGTGTGGAGGTTTCGGTACTCTTACTGGAACAGCAGTCAAAGCTACGTGTTGACCAAAGGGTGGAGCCGGTTCGTGAAGGAGAAGAATCTGATGGCCGGGGACATTGTGAGCTTTCAGAGGTCGACCGGACCGGACAAGCAGCTGTACATTGATTGGAAGGCCAGGATGAGTGTGAATAATACCAACAATAATGGGTCAAGCCCGGTTCAGGTTGGTCCGGTTCCGATGGTTCGGCTATTTGGGGTCAACATATTCAAAATACCTGGGAGCAGCGGGCCCGGGTCAGCGGATGCAGCTGCAGCTGCTGCTATCGGCGGAGGTTGCAATAATAATATTGGAAAGAGAATGAGAGAGATGGAGTTACTGGAATTAGAGTTTGGCAAGAAGCCAAGGATCATTGGAGCTTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0010033 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048364 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048527 GO:0048528 GO:0048573 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090696 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2 B3

Protein Analysis

407

Amino Acids

43.94

Weight (kDa)

8.92

Isoelectric Point (pI)

44.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AP2	PF00847	76 - 124	3e-09	AP2 domain
B3	PF02362	213 - 320	7.7e-25	B3 DNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13260 AT1G25560 AT1G68840 AT1G68840 AT3G25730
fragaria_vesca	FvH4_4g28030 FvH4_6g45390
malus_domestica	MD09G1070900.v1.1 MD09G1083900.v1.1 MD09G1084000.v1.1 MD09G1084100.v1.1 MD09G1088400.v1.1 MD13G1046100.v1.1 MD16G1047700.v1.1 MD17G1073700.v1.1 MD17G1073800.v1.1
prunus_persica	Prupe.1G305900_v2.0.a1 Prupe.3G240000_v2.0.a1
pyrus_communis	pycom09g01430 pycom16g04170
rosa_chinensis	RchiOBHm_Chr2g0163201 RchiOBHm_Chr4g0435771
rosa_laevigata	RLG00000006560 RLG00000021381
rosa_multiflora	Rmu_sc0002045.1_g000037 Rmu_ssc0000471.1_g000001
rosa_roxburghii	Rroxscaffold_2G00087470 Rroxscaffold_5G00376950
rosa_rugosa	Rorug02G0501100 Rorug02G0501200 Rorug04G0289500
rosa_samantha	Rh2AG567700 Rh2BG579800 Rh2CG549700 Rh2DG590100 Rh4AG343800 Rh4BG352500 Rh4CG367000 Rh4DG346400
rosa_wichuraiana	Rw2G047000 Rw4G030020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	308
AccB1I	GGYRCC	3 cut(s)	140, 269, 308
AccB7I	CCANNNNNTGG	1 cut(s)	870
AccI	GTMKAC	2 cut(s)	489, 932
AccII	CGCG	6 cut(s)	129, 297, 375, 455, 625, 704
AclWI	GGATC	3 cut(s)	586, 682, 1211
AcoI	YGGCCR	3 cut(s)	114, 370, 903
AcsI	RAATTY	1 cut(s)	787
AcvI	CACGTG	1 cut(s)	80
AcyI	GRCGYC	4 cut(s)	141, 195, 378, 444
AfaI	GTAC	5 cut(s)	233, 310, 598, 827, 956
AfeI	AGCGCT	3 cut(s)	362, 736, 757
AfiI	CCNNNNNNNGG	4 cut(s)	210, 555, 870, 1018
AflIII	ACRYGT	2 cut(s)	79, 855
AgsI	TTSAA	3 cut(s)	316, 787, 1066
AjnI	CCWGG	4 cut(s)	227, 335, 971, 1072
AjuI	GAANNNNNNNTTGG	2 cut(s)	1170, 1202
Alw21I	GWGCWC	3 cut(s)	32, 171, 467
AlwI	GGATC	3 cut(s)	586, 682, 1211
AlwNI	CAGNNNCTG	2 cut(s)	123, 1115
Ama87I	CYCGRG	3 cut(s)	164, 655, 1088
Aor51HI	AGCGCT	3 cut(s)	362, 736, 757
ApaI	GGGCCC	1 cut(s)	1089
ApoI	RAATTY	1 cut(s)	787
ArsI	GACNNNNNNTTYG	2 cut(s)	228, 260
Asp718I	GGTACC	1 cut(s)	308
AspLEI	GCGC	7 cut(s)	143, 297, 363, 540, 625, 737, 758
AsuC2I	CCSGG	6 cut(s)	211, 568, 907, 1013, 1089, 1090
AsuHPI	GGTGA	2 cut(s)	376, 661
AsuII	TTCGAA	1 cut(s)	639
AvaI	CYCGRG	3 cut(s)	164, 655, 1088
AvaII	GGWCC	6 cut(s)	85, 244, 564, 570, 938, 1025
BaeGI	GKGCMC	2 cut(s)	274, 1089
BalI	TGGCCA	1 cut(s)	116
BanI	GGYRCC	3 cut(s)	140, 269, 308
BanII	GRGCYC	3 cut(s)	171, 467, 1089
BauI	CACGAG	1 cut(s)	683
BbrPI	CACGTG	1 cut(s)	80
Bbv12I	GWGCWC	3 cut(s)	32, 171, 467
BccI	CCATC	3 cut(s)	895, 1030, 1159
BceAI	ACGGC	1 cut(s)	365
BcgI	CGANNNNNNTGC	2 cut(s)	335, 369
BciT130I	CCWGG	4 cut(s)	229, 337, 973, 1074
BcnI	CCSGG	6 cut(s)	211, 568, 907, 1013, 1089, 1090
BfmI	CTRYAG	1 cut(s)	1107
BfoI	RGCGCY	4 cut(s)	144, 364, 738, 759
BglI	GCCNNNNNGGC	2 cut(s)	132, 338
BlpI	GCTNAGC	1 cut(s)	170
Bme18I	GGWCC	6 cut(s)	85, 244, 564, 570, 938, 1025
BmeT110I	CYCGRG	3 cut(s)	164, 655, 1088
BmiI	GGNNCC	9 cut(s)	87, 142, 246, 271, 310, 566, 875, 1032, 1087
BmrI	ACTGGG	1 cut(s)	593
BmsI	GCATC	1 cut(s)	1090
BmuI	ACTGGG	1 cut(s)	593
BpmI	CTGGAG	1 cut(s)	211
Bpu1102I	GCTNAGC	1 cut(s)	170
Bpu14I	TTCGAA	1 cut(s)	639
BpuMI	CCSGG	6 cut(s)	211, 568, 907, 1013, 1089, 1090
BsaAI	YACGTR	2 cut(s)	80, 856
BsaHI	GRCGYC	4 cut(s)	141, 195, 378, 444
BsaJI	CCNNGG	7 cut(s)	336, 367, 447, 906, 1073, 1088, 1199
BsaWI	WCCGGW	3 cut(s)	935, 940, 1027
Bsc4I	CCNNNNNNNGG	4 cut(s)	210, 555, 870, 1018
Bse118I	RCCGGY	1 cut(s)	876
Bse1I	ACTGG	4 cut(s)	193, 588, 839, 1179
BseBI	CCWGG	4 cut(s)	229, 337, 973, 1074
BseDI	CCNNGG	7 cut(s)	336, 367, 447, 906, 1073, 1088, 1199
BseGI	GGATG	4 cut(s)	401, 802, 981, 1105
BseLI	CCNNNNNNNGG	4 cut(s)	210, 555, 870, 1018
BseMII	CTCAG	2 cut(s)	184, 452
BseNI	ACTGG	4 cut(s)	193, 588, 839, 1179
BseSI	GKGCMC	2 cut(s)	274, 1089
BseYI	CCCAGC	2 cut(s)	264, 304
BsgI	GTGCAG	1 cut(s)	339
Bsh1236I	CGCG	6 cut(s)	129, 297, 375, 455, 625, 704
Bsh1285I	CGRYCG	1 cut(s)	936
BshNI	GGYRCC	3 cut(s)	140, 269, 308
BsiEI	CGRYCG	1 cut(s)	936
BsiHKAI	GWGCWC	3 cut(s)	32, 171, 467
BsiHKCI	CYCGRG	3 cut(s)	164, 655, 1088
BsiSI	CCGG	9 cut(s)	211, 568, 877, 906, 936, 941, 1013, 1028, 1089
BslFI	GGGAC	5 cut(s)	193, 230, 550, 599, 923
BslI	CCNNNNNNNGG	4 cut(s)	210, 555, 870, 1018
BsmFI	GGGAC	5 cut(s)	193, 230, 550, 599, 923
BsoBI	CYCGRG	3 cut(s)	164, 655, 1088
Bsp119I	TTCGAA	1 cut(s)	639
Bsp120I	GGGCCC	1 cut(s)	1085
Bsp1286I	GDGCHC	5 cut(s)	32, 171, 274, 467, 1089
Bsp1407I	TGTACA	1 cut(s)	954
Bsp143I	GATC	3 cut(s)	578, 687, 1203
Bsp1720I	GCTNAGC	1 cut(s)	170
BspCNI	CTCAG	2 cut(s)	183, 453
BspFNI	CGCG	6 cut(s)	129, 297, 375, 455, 625, 704
BspLI	GGNNCC	9 cut(s)	87, 142, 246, 271, 310, 566, 875, 1032, 1087
BspMAI	CTGCAG	1 cut(s)	1111
BspPI	GGATC	3 cut(s)	586, 682, 1211
BspT104I	TTCGAA	1 cut(s)	639
BspT107I	GGYRCC	3 cut(s)	140, 269, 308
BsrFI	RCCGGY	1 cut(s)	876
BsrGI	TGTACA	1 cut(s)	954
BsrI	ACTGG	4 cut(s)	193, 588, 839, 1179
BssAI	RCCGGY	1 cut(s)	876
BssECI	CCNNGG	7 cut(s)	336, 367, 447, 906, 1073, 1088, 1199
BssMI	GATC	3 cut(s)	578, 687, 1203
BssNI	GRCGYC	4 cut(s)	141, 195, 378, 444
BssSI	CACGAG	1 cut(s)	683
BssT1I	CCWWGG	1 cut(s)	1199
Bst2BI	CACGAG	1 cut(s)	683
Bst2UI	CCWGG	4 cut(s)	229, 337, 973, 1074
Bst4CI	ACNGT	2 cut(s)	608, 751
Bst6I	CTCTTC	2 cut(s)	18, 315
BstACI	GRCGYC	4 cut(s)	141, 195, 378, 444
BstAPI	GCANNNNNTGC	1 cut(s)	1115
BstAUI	TGTACA	1 cut(s)	954
BstBAI	YACGTR	2 cut(s)	80, 856
BstBI	TTCGAA	1 cut(s)	639
BstC8I	GCNNGC	6 cut(s)	118, 145, 536, 702, 769, 1085
BstDEI	CTNAG	3 cut(s)	170, 461, 474
BstDSI	CCRYGG	1 cut(s)	367
BstEII	GGTNACC	1 cut(s)	649
BstF5I	GGATG	4 cut(s)	401, 802, 981, 1105
BstFNI	CGCG	6 cut(s)	129, 297, 375, 455, 625, 704
BstH2I	RGCGCY	4 cut(s)	144, 364, 738, 759
BstHHI	GCGC	7 cut(s)	143, 297, 363, 540, 625, 737, 758
BstKTI	GATC	3 cut(s)	581, 690, 1206
BstMBI	GATC	3 cut(s)	578, 687, 1203
BstMCI	CGRYCG	1 cut(s)	936
BstNI	CCWGG	4 cut(s)	229, 337, 973, 1074
BstNSI	RCATGY	1 cut(s)	496
BstPI	GGTNACC	1 cut(s)	649
BstSFI	CTRYAG	1 cut(s)	1107
BstSLI	GKGCMC	2 cut(s)	274, 1089
BstUI	CGCG	6 cut(s)	129, 297, 375, 455, 625, 704
BtgI	CCRYGG	1 cut(s)	367
BtgZI	GCGATG	1 cut(s)	675
BtsCI	GGATG	4 cut(s)	401, 802, 981, 1105
BtsI	GCAGTG	1 cut(s)	154
BtsIMutI	CAGTG	4 cut(s)	154, 186, 581, 613
Cac8I	GCNNGC	6 cut(s)	118, 145, 536, 702, 769, 1085
CaiI	CAGNNNCTG	2 cut(s)	123, 1115
CfoI	GCGC	7 cut(s)	143, 297, 363, 540, 625, 737, 758
Cfr10I	RCCGGY	1 cut(s)	876
Cfr9I	CCCGGG	1 cut(s)	1088
CpoI	CGGWCCG	1 cut(s)	938
CseI	GACGC	6 cut(s)	118, 184, 270, 286, 386, 452
Csp6I	GTAC	5 cut(s)	232, 309, 597, 826, 955
CspI	CGGWCCG	1 cut(s)	938
CviAII	CATG	1 cut(s)	493
CviQI	GTAC	5 cut(s)	232, 309, 597, 826, 955
DdeI	CTNAG	3 cut(s)	170, 461, 474
DinI	GGCGCC	1 cut(s)	142
DpnI	GATC	3 cut(s)	580, 689, 1205
DpnII	GATC	3 cut(s)	578, 687, 1203
EaeI	YGGCCR	3 cut(s)	114, 370, 903
Eam1104I	CTCTTC	2 cut(s)	18, 315
EarI	CTCTTC	2 cut(s)	18, 315
EciI	GGCGGA	2 cut(s)	394, 1140
Ecl136II	GAGCTC	2 cut(s)	169, 465
Eco130I	CCWWGG	1 cut(s)	1199
Eco24I	GRGCYC	3 cut(s)	171, 467, 1089
Eco47I	GGWCC	6 cut(s)	85, 244, 564, 570, 938, 1025
Eco47III	AGCGCT	3 cut(s)	362, 736, 757
Eco53kI	GAGCTC	2 cut(s)	169, 465
Eco72I	CACGTG	1 cut(s)	80
Eco88I	CYCGRG	3 cut(s)	164, 655, 1088
Eco91I	GGTNACC	1 cut(s)	649
EcoICRI	GAGCTC	2 cut(s)	169, 465
EcoO109I	RGGNCCY	1 cut(s)	339
EcoO65I	GGTNACC	1 cut(s)	649
EcoRII	CCWGG	4 cut(s)	227, 335, 971, 1072
EcoT14I	CCWWGG	1 cut(s)	1199
EcoT38I	GRGCYC	3 cut(s)	171, 467, 1089
EgeI	GGCGCC	1 cut(s)	142
EheI	GGCGCC	1 cut(s)	142
ErhI	CCWWGG	1 cut(s)	1199
FaeI	CATG	1 cut(s)	496
FaiI	YATR	4 cut(s)	47, 69, 494, 1061
FalI	AAGNNNNNCTT	2 cut(s)	765, 797
FaqI	GGGAC	5 cut(s)	193, 230, 550, 599, 923
FatI	CATG	1 cut(s)	492
FauI	CCCGC	2 cut(s)	255, 1076
FblI	GTMKAC	2 cut(s)	489, 932
FokI	GGATG	4 cut(s)	388, 809, 988, 1112
FriOI	GRGCYC	3 cut(s)	171, 467, 1089
GlaI	GCGC	7 cut(s)	142, 296, 362, 539, 624, 736, 757
GsaI	CCCAGC	2 cut(s)	268, 308
GsuI	CTGGAG	1 cut(s)	211
HaeII	RGCGCY	4 cut(s)	144, 364, 738, 759
HapII	CCGG	9 cut(s)	211, 568, 877, 906, 936, 941, 1013, 1028, 1089
HgaI	GACGC	6 cut(s)	118, 184, 270, 286, 386, 452
HhaI	GCGC	7 cut(s)	143, 297, 363, 540, 625, 737, 758
Hin1I	GRCGYC	4 cut(s)	141, 195, 378, 444
Hin1II	CATG	1 cut(s)	496
Hin6I	GCGC	7 cut(s)	141, 295, 361, 538, 623, 735, 756
HinP1I	GCGC	7 cut(s)	141, 295, 361, 538, 623, 735, 756
HincII	GTYRAC	4 cut(s)	490, 861, 933, 1057
HindII	GTYRAC	4 cut(s)	490, 861, 933, 1057
HinfI	GANTC	5 cut(s)	41, 154, 206, 612, 895
HpaII	CCGG	9 cut(s)	211, 568, 877, 906, 936, 941, 1013, 1028, 1089
HphI	GGTGA	2 cut(s)	376, 661
Hpy166II	GTNNAC	5 cut(s)	85, 490, 861, 933, 1057
Hpy188I	TCNGA	6 cut(s)	424, 462, 481, 900, 927, 1035
Hpy188III	TCNNGA	6 cut(s)	158, 616, 667, 685, 691, 883
Hpy8I	GTNNAC	5 cut(s)	85, 490, 861, 933, 1057
Hpy99I	CGWCG	9 cut(s)	134, 200, 350, 380, 428, 431, 443, 446, 491
HpyAV	CCTTC	4 cut(s)	231, 322, 880, 961
HpyCH4III	ACNGT	2 cut(s)	608, 751
HpyCH4IV	ACGT	3 cut(s)	79, 348, 855
HpyCH4V	TGCA	8 cut(s)	77, 356, 724, 740, 771, 1103, 1109, 1134
HpyF3I	CTNAG	3 cut(s)	170, 461, 474
HpySE526I	ACGT	3 cut(s)	79, 348, 855
Hsp92I	GRCGYC	4 cut(s)	141, 195, 378, 444
Hsp92II	CATG	1 cut(s)	496
HspAI	GCGC	7 cut(s)	141, 295, 361, 538, 623, 735, 756
KasI	GGCGCC	1 cut(s)	140
KpnI	GGTACC	1 cut(s)	312
Kzo9I	GATC	3 cut(s)	578, 687, 1203
LmnI	GCTCC	5 cut(s)	222, 470, 873, 1077, 1211
LweI	GCATC	1 cut(s)	1090
MaeII	ACGT	3 cut(s)	79, 348, 855
MaeIII	GTNAC	3 cut(s)	382, 649, 1170
MalI	GATC	3 cut(s)	580, 689, 1205
MboI	GATC	3 cut(s)	578, 687, 1203
MboII	GAAGA	4 cut(s)	35, 332, 443, 904
MhlI	GDGCHC	5 cut(s)	32, 171, 274, 467, 1089
MlsI	TGGCCA	1 cut(s)	116
MluCI	AATT	2 cut(s)	787, 1178
MluNI	TGGCCA	1 cut(s)	116
Mly113I	GGCGCC	1 cut(s)	141
MlyI	GAGTC	3 cut(s)	35, 215, 621
MmeI	TCCRAC	3 cut(s)	88, 447, 597
Mox20I	TGGCCA	1 cut(s)	116
MscI	TGGCCA	1 cut(s)	116
MseI	TTAA	2 cut(s)	393, 746
Msp20I	TGGCCA	1 cut(s)	116
MspA1I	CMGCKG	7 cut(s)	138, 180, 952, 1083, 1097, 1106, 1112
MspI	CCGG	9 cut(s)	211, 568, 877, 906, 936, 941, 1013, 1028, 1089
MvaI	CCWGG	4 cut(s)	229, 337, 973, 1074
MvnI	CGCG	6 cut(s)	129, 297, 375, 455, 625, 704
NarI	GGCGCC	1 cut(s)	141
NciI	CCSGG	6 cut(s)	211, 568, 907, 1013, 1089, 1090
NdeII	GATC	3 cut(s)	578, 687, 1203
NlaIII	CATG	1 cut(s)	496
NlaIV	GGNNCC	9 cut(s)	87, 142, 246, 271, 310, 566, 875, 1032, 1087
NmeAIII	GCCGAG	2 cut(s)	229, 472
NmuCI	GTSAC	2 cut(s)	382, 649
NspI	RCATGY	1 cut(s)	496
NspV	TTCGAA	1 cut(s)	639
PcsI	WCGNNNNNNNCGW	3 cut(s)	324, 435, 444
PfeI	GAWTC	2 cut(s)	154, 895
PflFI	GACNNNGTC	1 cut(s)	380
PflMI	CCANNNNNTGG	1 cut(s)	870
PfoI	TCCNGGA	1 cut(s)	209
PleI	GAGTC	3 cut(s)	35, 214, 620
PluTI	GGCGCC	1 cut(s)	144
PmaCI	CACGTG	1 cut(s)	80
PmlI	CACGTG	1 cut(s)	80
PpsI	GAGTC	3 cut(s)	35, 214, 620
Ppu21I	YACGTR	2 cut(s)	80, 856
Psp124BI	GAGCTC	2 cut(s)	171, 467
Psp6I	CCWGG	4 cut(s)	227, 335, 971, 1072
PspCI	CACGTG	1 cut(s)	80
PspEI	GGTNACC	1 cut(s)	649
PspFI	CCCAGC	2 cut(s)	264, 304
PspGI	CCWGG	4 cut(s)	227, 335, 971, 1072
PspN4I	GGNNCC	9 cut(s)	87, 142, 246, 271, 310, 566, 875, 1032, 1087
PspOMI	GGGCCC	1 cut(s)	1085
PstI	CTGCAG	1 cut(s)	1111
PstNI	CAGNNNCTG	2 cut(s)	123, 1115
PsyI	GACNNNGTC	1 cut(s)	380
PvuII	CAGCTG	3 cut(s)	952, 1106, 1112
RsaI	GTAC	5 cut(s)	233, 310, 598, 827, 956
RsaNI	GTAC	5 cut(s)	232, 309, 597, 826, 955
Rsr2I	CGGWCCG	1 cut(s)	938
RsrII	CGGWCCG	1 cut(s)	938
SacI	GAGCTC	2 cut(s)	171, 467
SalI	GTCGAC	2 cut(s)	488, 931
SaqAI	TTAA	2 cut(s)	393, 746
Sau3AI	GATC	3 cut(s)	578, 687, 1203
SchI	GAGTC	3 cut(s)	35, 215, 621
SduI	GDGCHC	5 cut(s)	32, 171, 274, 467, 1089
SfaNI	GCATC	1 cut(s)	1090
SfcI	CTRYAG	1 cut(s)	1107
SfiI	GGCCNNNNNGGCC	1 cut(s)	338
SfoI	GGCGCC	1 cut(s)	142
SfuI	TTCGAA	1 cut(s)	639
SinI	GGWCC	6 cut(s)	85, 244, 564, 570, 938, 1025
SmaI	CCCGGG	1 cut(s)	1090
Sse9I	AATT	2 cut(s)	787, 1178
SspDI	GGCGCC	1 cut(s)	140
SspI	AATATT	1 cut(s)	1144
SstI	GAGCTC	2 cut(s)	171, 467
StyI	CCWWGG	1 cut(s)	1199
TaaI	ACNGT	2 cut(s)	608, 751
TaiI	ACGT	3 cut(s)	82, 351, 858
TaqI	TCGA	5 cut(s)	159, 198, 489, 639, 932
TasI	AATT	2 cut(s)	787, 1178
TatI	WGTACW	1 cut(s)	954
TauI	GCSGC	8 cut(s)	129, 141, 183, 186, 195, 335, 375, 455
TfiI	GAWTC	2 cut(s)	154, 895
Tru1I	TTAA	2 cut(s)	393, 746
Tru9I	TTAA	2 cut(s)	393, 746
TscAI	CASTG	4 cut(s)	154, 193, 588, 613
TseFI	GTSAC	2 cut(s)	382, 649
Tsp45I	GTSAC	2 cut(s)	382, 649
TspGWI	ACGGA	5 cut(s)	21, 273, 356, 591, 614
TspMI	CCCGGG	1 cut(s)	1088
TspRI	CASTG	4 cut(s)	154, 193, 588, 613
Tth111I	GACNNNGTC	1 cut(s)	380
Van91I	CCANNNNNTGG	1 cut(s)	870
VpaK11BI	GGWCC	6 cut(s)	85, 244, 564, 570, 938, 1025
XapI	RAATTY	1 cut(s)	787
XceI	RCATGY	1 cut(s)	496
XcmI	CCANNNNNNNNNTGG	1 cut(s)	580
XmaI	CCCGGG	1 cut(s)	1088
XmiI	GTMKAC	2 cut(s)	489, 932

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.