Rroxscaffold_2G00087470
ERF Family

AP2 ERF and B3 domain-containing transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002443
Physical Location & Seq
Forward (+)
9529403 .. 9531706
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087470.1

Sequence Viewer

Length: 912 bp
ATGGATTTGATGAGCAGTTTCAATGAGAATGCAAGCGATTCAACTTCCAAGTTACCTTCTTCACGGTACAAAGGAGTAGTCCCGCAGCCTAATGGCCGTTGGGGAGCGCAGATATACGAGAAGCACCAGAGAGTATGGTTGGGAACGTTCAACGGAGAAGAAGAAGCTGCCAAGGCATACGACATTGCTTCACAAAGGTTCCGAGGCCTCGACGCCATCACGAACTTCAAGCAATCCTCATGGAGTGCCACCATAGATGATCATGAAGATGAGGCCTTCTTCTTGCAGTCACATTCCAAGGCCGAGATCGTTGACATGCTTCGAAAACACTCCTACACCAATGAGCTAGAGCTGTTCAAGCACAAGAATCGTGGCAAGAGAATAAAGTACCGTAACATTGATTTGATGGACATTCATATTCAGAAGGAATGGCTGTTCGAGAAGGTGCTAACACCAAGCGATGTGGGGAGGCTAAACCGGTTTGTCATACCGAAACAACATGCCGAGAAGCATTTTCCGTTGATGGATTCTGAAGCTAAGGGAGCGTTACTAAATTTTGAGGATGATTTGGGGAATGTGTGGAGGTTTAGGTATTGTTTTTGGAGTAGCAGTCAGAGTTATGTGTTGACAAAAGGGTGGACTCGGTTTGTGCAGGAGAAGAAGCTGAAAGGGGGTGATGTAATTAGGTTTCATGGATCAAGTATGGGAGAGGATAAGAAGTTGTTCATAGAGTGTAGGCCAAGAAACATCGGCGATGAGGTTTCGGGTTTTCCGGAGAGGACTAGTACGGTCGATGAGCCATTAATGGCGCCGGGTCGAGATCACGATGGAGTGGTAAGGTTGTTTGGAGTTAACATTGTTGATAGCAACATTTTAGACAATAGCACATACAATTGCTCTCAATATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

303

Amino Acids

35.03

Weight (kDa)

6.81

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 21 - 69 4.9e-09 AP2 domain
B3 PF02362 146 - 248 2.6e-25 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 808
AccIII TCCGGA 1 cut(s) 772
AciI CCGC 1 cut(s) 83
AclI AACGTT 1 cut(s) 146
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 2 cut(s) 213, 809
AfaI GTAC 3 cut(s) 68, 389, 787
AgeI ACCGGT 1 cut(s) 477
AgsI TTSAA 5 cut(s) 22, 42, 151, 229, 358
AhlI ACTAGT 1 cut(s) 782
AloI GAACNNNNNNTCC 2 cut(s) 419, 451
AluBI AGCT 5 cut(s) 167, 346, 352, 536, 664
AluI AGCT 5 cut(s) 167, 346, 352, 536, 664
AlwI GGATC 1 cut(s) 703
Aor13HI TCCGGA 1 cut(s) 772
AoxI GGCC 5 cut(s) 94, 205, 273, 300, 737
ApeKI GCWGC 2 cut(s) 85, 167
ApoI RAATTY 1 cut(s) 553
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
AseI ATTAAT 1 cut(s) 803
AsiGI ACCGGT 1 cut(s) 477
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 2 cut(s) 109, 811
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 1 cut(s) 686
AsuII TTCGAA 1 cut(s) 322
BanI GGYRCC 1 cut(s) 808
BbvI GCAGC 2 cut(s) 97, 154
BccI CCATC 4 cut(s) 224, 400, 517, 821
BceAI ACGGC 1 cut(s) 81
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BclI TGATCA 1 cut(s) 259
BcnI CCSGG 1 cut(s) 813
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 2 cut(s) 347, 783
BfoI RGCGCY 1 cut(s) 812
BisI GCNGC 2 cut(s) 86, 168
BlsI GCNGC 2 cut(s) 87, 169
Bme1390I CCNGG 1 cut(s) 813
BmiI GGNNCC 2 cut(s) 200, 810
BmrFI CCNGG 1 cut(s) 813
Bpu10I CCTNAGC 1 cut(s) 537
Bpu14I TTCGAA 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 813
BsaHI GRCGYC 2 cut(s) 213, 809
BsaJI CCNNGG 3 cut(s) 171, 202, 297
BsaWI WCCGGW 2 cut(s) 477, 772
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bse118I RCCGGY 1 cut(s) 477
Bse3DI GCAATG 1 cut(s) 183
BseAI TCCGGA 1 cut(s) 772
BseDI CCNNGG 3 cut(s) 171, 202, 297
BseGI GGATG 1 cut(s) 568
BseMI GCAATG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 97, 154
BsgI GTGCAG 1 cut(s) 671
Bsh1285I CGRYCG 1 cut(s) 792
BshFI GGCC 5 cut(s) 96, 207, 275, 302, 739
BshNI GGYRCC 1 cut(s) 808
BshTI ACCGGT 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 792
BsiSI CCGG 3 cut(s) 478, 773, 812
BslFI GGGAC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 5 cut(s) 96, 207, 275, 302, 739
Bsp119I TTCGAA 1 cut(s) 322
Bsp13I TCCGGA 1 cut(s) 772
Bsp143I GATC 4 cut(s) 259, 306, 695, 820
BspACI CCGC 1 cut(s) 83
BspANI GGCC 5 cut(s) 96, 207, 275, 302, 739
BspEI TCCGGA 1 cut(s) 772
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 200, 810
BspPI GGATC 1 cut(s) 703
BspT104I TTCGAA 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 808
BsrDI GCAATG 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 477
BssAI RCCGGY 1 cut(s) 477
BssECI CCNNGG 3 cut(s) 171, 202, 297
BssMI GATC 4 cut(s) 259, 306, 695, 820
BssNI GRCGYC 2 cut(s) 213, 809
BssT1I CCWWGG 2 cut(s) 171, 297
Bst4CI ACNGT 3 cut(s) 66, 392, 790
BstACI GRCGYC 2 cut(s) 213, 809
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 537
BstF5I GGATG 1 cut(s) 568
BstH2I RGCGCY 1 cut(s) 812
BstHHI GCGC 2 cut(s) 109, 811
BstKTI GATC 4 cut(s) 262, 309, 698, 823
BstMBI GATC 4 cut(s) 259, 306, 695, 820
BstMCI CGRYCG 1 cut(s) 792
BstMWI GCNNNNNNNGC 3 cut(s) 173, 358, 542
BstNSI RCATGY 2 cut(s) 319, 503
BstSCI CCNGG 1 cut(s) 811
BstV1I GCAGC 2 cut(s) 97, 154
BsuRI GGCC 5 cut(s) 96, 207, 275, 302, 739
BtgZI GCGATG 2 cut(s) 474, 768
BtsCI GGATG 1 cut(s) 568
Cac8I GCNNGC 1 cut(s) 34
CciI TCATGA 1 cut(s) 262
CfoI GCGC 2 cut(s) 109, 811
Cfr10I RCCGGY 1 cut(s) 477
CseI GACGC 1 cut(s) 221
Csp6I GTAC 3 cut(s) 67, 388, 786
CspAI ACCGGT 1 cut(s) 477
CspCI CAANNNNNGTGG 4 cut(s) 352, 387, 444, 479
CviAII CATG 5 cut(s) 240, 263, 316, 500, 692
CviQI GTAC 3 cut(s) 67, 388, 786
DdeI CTNAG 1 cut(s) 537
DinI GGCGCC 1 cut(s) 810
DpnI GATC 4 cut(s) 261, 308, 697, 822
DpnII GATC 4 cut(s) 259, 306, 695, 820
EaeI YGGCCR 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 171, 297
Eco147I AGGCCT 2 cut(s) 207, 275
Eco57I CTGAAG 1 cut(s) 552
EcoT14I CCWWGG 2 cut(s) 171, 297
EgeI GGCGCC 1 cut(s) 810
EheI GGCGCC 1 cut(s) 810
ErhI CCWWGG 2 cut(s) 171, 297
FaeI CATG 5 cut(s) 243, 266, 319, 503, 695
FaqI GGGAC 1 cut(s) 65
FatI CATG 5 cut(s) 239, 262, 315, 499, 691
FauI CCCGC 1 cut(s) 90
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 2 cut(s) 86, 168
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 2 cut(s) 86, 168
FspBI CTAG 2 cut(s) 347, 783
GlaI GCGC 2 cut(s) 108, 810
GluI GCNGC 2 cut(s) 86, 168
HaeII RGCGCY 1 cut(s) 812
HaeIII GGCC 5 cut(s) 96, 207, 275, 302, 739
HapII CCGG 3 cut(s) 478, 773, 812
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 109, 811
Hin1I GRCGYC 2 cut(s) 213, 809
Hin1II CATG 5 cut(s) 243, 266, 319, 503, 695
Hin6I GCGC 2 cut(s) 107, 809
HinP1I GCGC 2 cut(s) 107, 809
HincII GTYRAC 3 cut(s) 313, 627, 853
HindII GTYRAC 3 cut(s) 313, 627, 853
HinfI GANTC 4 cut(s) 38, 367, 527, 640
HpaI GTTAAC 1 cut(s) 853
HpaII CCGG 3 cut(s) 478, 773, 812
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 4 cut(s) 313, 627, 639, 853
Hpy188I TCNGA 4 cut(s) 203, 423, 532, 615
Hpy188III TCNNGA 6 cut(s) 220, 263, 439, 773, 818, 824
Hpy8I GTNNAC 4 cut(s) 313, 627, 639, 853
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 66, 286, 418, 436
HpyCH4III ACNGT 3 cut(s) 66, 392, 790
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 3 cut(s) 32, 286, 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 358, 542
HpyF3I CTNAG 1 cut(s) 537
HpySE526I ACGT 1 cut(s) 146
Hsp92I GRCGYC 2 cut(s) 213, 809
Hsp92II CATG 5 cut(s) 243, 266, 319, 503, 695
HspAI GCGC 2 cut(s) 107, 809
KasI GGCGCC 1 cut(s) 808
Kpn2I TCCGGA 1 cut(s) 772
Ksp22I TGATCA 1 cut(s) 259
KspAI GTTAAC 1 cut(s) 853
Kzo9I GATC 4 cut(s) 259, 306, 695, 820
LmnI GCTCC 2 cut(s) 104, 542
LpnPI CCDG 5 cut(s) 140, 491, 638, 786, 825
Lsp1109I GCAGC 2 cut(s) 97, 154
MaeI CTAG 2 cut(s) 347, 783
MaeII ACGT 1 cut(s) 146
MaeIII GTNAC 4 cut(s) 51, 288, 392, 546
MalI GATC 4 cut(s) 261, 308, 697, 822
MboI GATC 4 cut(s) 259, 306, 695, 820
MboII GAAGA 6 cut(s) 51, 170, 173, 271, 278, 670
MfeI CAATTG 1 cut(s) 892
MluCI AATT 3 cut(s) 553, 681, 892
Mly113I GGCGCC 1 cut(s) 809
MlyI GAGTC 1 cut(s) 634
MroI TCCGGA 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 2 cut(s) 803, 852
MslI CAYNNNNRTG 1 cut(s) 267
MspI CCGG 3 cut(s) 478, 773, 812
MspR9I CCNGG 1 cut(s) 813
MunI CAATTG 1 cut(s) 892
Mva1269I GAATGC 1 cut(s) 34
MwoI GCNNNNNNNGC 3 cut(s) 173, 358, 542
NarI GGCGCC 1 cut(s) 809
NciI CCSGG 1 cut(s) 813
NdeII GATC 4 cut(s) 259, 306, 695, 820
NlaIII CATG 5 cut(s) 243, 266, 319, 503, 695
NlaIV GGNNCC 2 cut(s) 200, 810
NmeAIII GCCGAG 2 cut(s) 328, 529
NmuCI GTSAC 1 cut(s) 288
NspI RCATGY 2 cut(s) 319, 503
NspV TTCGAA 1 cut(s) 322
PagI TCATGA 1 cut(s) 262
PceI AGGCCT 2 cut(s) 207, 275
PctI GAATGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 3 cut(s) 38, 367, 527
PinAI ACCGGT 1 cut(s) 477
PkrI GCNGC 2 cut(s) 87, 169
PleI GAGTC 1 cut(s) 634
PluTI GGCGCC 1 cut(s) 812
PpsI GAGTC 1 cut(s) 634
PshBI ATTAAT 1 cut(s) 803
Psp1406I AACGTT 1 cut(s) 146
PspN4I GGNNCC 2 cut(s) 200, 810
RsaI GTAC 3 cut(s) 68, 389, 787
RsaNI GTAC 3 cut(s) 67, 388, 786
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 2 cut(s) 803, 852
SatI GCNGC 2 cut(s) 86, 168
Sau3AI GATC 4 cut(s) 259, 306, 695, 820
SchI GAGTC 1 cut(s) 634
ScrFI CCNGG 1 cut(s) 813
SfoI GGCGCC 1 cut(s) 810
SfuI TTCGAA 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 267
SpeI ACTAGT 1 cut(s) 782
Sse9I AATT 3 cut(s) 553, 681, 892
SseBI AGGCCT 2 cut(s) 207, 275
SsiI CCGC 1 cut(s) 83
SspDI GGCGCC 1 cut(s) 808
SspMI CTAG 2 cut(s) 347, 783
StuI AGGCCT 2 cut(s) 207, 275
StyD4I CCNGG 1 cut(s) 811
StyI CCWWGG 2 cut(s) 171, 297
TaaI ACNGT 3 cut(s) 66, 392, 790
TaiI ACGT 1 cut(s) 149
TaqI TCGA 5 cut(s) 210, 322, 438, 792, 817
TasI AATT 3 cut(s) 553, 681, 892
TfiI GAWTC 3 cut(s) 38, 367, 527
Tru1I TTAA 2 cut(s) 803, 852
Tru9I TTAA 2 cut(s) 803, 852
TseFI GTSAC 1 cut(s) 288
TseI GCWGC 2 cut(s) 85, 167
Tsp45I GTSAC 1 cut(s) 288
TspDTI ATGAA 4 cut(s) 279, 404, 680, 715
TspGWI ACGGA 2 cut(s) 168, 507
VspI ATTAAT 1 cut(s) 803
XapI RAATTY 1 cut(s) 553
XceI RCATGY 2 cut(s) 319, 503
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 2 cut(s) 347, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.