pycom09g01430
ERF Family

AP2 ERF and B3 domain-containing transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002443
Physical Location & Seq
Forward (+)
1103800 .. 1104658
859 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01430.1

Sequence Viewer

Length: 768 bp
ATGCCTTCTTCTAGGTACAAACGAGTAGTGCTCCAGTCAAATGGAGGATGGGGAGCTCAAATATACGAGAAGAGTCATCGTGTGGGGTTGGGAACCTTCAACGATGAAGAAGAAGCTGCCAAAACCTACGACATTGCCTCGCTAAAGTTCCGGGGTCTTGATGCCATCACAAACAACAGTCGAAACAAAATGAAACCCTACGAACGTGATGTCACCGAGGCTCTTTTCTTGGAGTCTCATTCCAAGGCTGAGATCGTTGACATGCTTCGAAAACATTCTTATGCCAACGAGCTCGAGATTTACAAGTATAAATTGTACAATGGTGGTGCCGTAGGCCTTGATGATGGTGGAAAGCGTATGAAGCATTACCTTGATTTAAGGGAAACGTGTAGTCGCGGGAGGGAGTTGCTTTTCGAGAAAGTGGAAACACCAAGCGCCGTAGGGAGATTGAACCGCATGGTTATACCAAAACAACATGCCGAGAAGCATTTTCAGGTTCATCTGAATATAGGGTTTGGCAAAGGGGTCTTGTTGAAATTTGAGGATGAGGAGAAGAAGCTGAAAGTTGGTGATGTTGTGAGGTTTGAGAGATCGGTAGGGGAGGATAAGAAGATGTTCATTTACTGTAGACTTAGAAACCTCGACATGTCGGGGATCAGAGAGATGCATGGTAGGGTCGGTGACCCTCTAAGGACGGTTCAAGATCATGGAGCGGTGAGGCTGTTCGGAGTTAACATATCACGGAAACCTGTGCATTGTAGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

256

Amino Acids

29.32

Weight (kDa)

9.41

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 326
AccBSI CCGCTC 1 cut(s) 713
AccI GTMKAC 1 cut(s) 628
AccII CGCG 1 cut(s) 396
AciI CCGC 3 cut(s) 396, 454, 713
AclWI GGATC 1 cut(s) 662
AcsI RAATTY 1 cut(s) 536
AfaI GTAC 2 cut(s) 17, 317
AflIII ACRYGT 2 cut(s) 386, 645
AgsI TTSAA 4 cut(s) 100, 451, 535, 701
AluBI AGCT 4 cut(s) 56, 116, 292, 559
AluI AGCT 4 cut(s) 56, 116, 292, 559
Alw21I GWGCWC 3 cut(s) 33, 58, 294
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 1 cut(s) 536
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
Asp700I GAANNNNTTC 2 cut(s) 274, 614
AspLEI GCGC 1 cut(s) 437
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 4 cut(s) 205, 581, 692, 727
AsuII TTCGAA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 293
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 2 cut(s) 58, 294
Bbv12I GWGCWC 3 cut(s) 33, 58, 294
BbvI GCAGC 1 cut(s) 103
BccI CCATC 3 cut(s) 42, 173, 338
BceAI ACGGC 2 cut(s) 314, 422
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 625
BfoI RGCGCY 1 cut(s) 438
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 1 cut(s) 152
BmeT110I CYCGRG 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 94, 328
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 2 cut(s) 151, 654
BplI GAGNNNNNCTC 2 cut(s) 15, 47
BpmI CTGGAG 1 cut(s) 17
Bpu14I TTCGAA 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 151, 216, 243
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 132
BseDI CCNNGG 3 cut(s) 151, 216, 243
BseGI GGATG 2 cut(s) 53, 550
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 563
BseXI GCAGC 1 cut(s) 103
Bsh1236I CGCG 1 cut(s) 396
BshFI GGCC 1 cut(s) 336
BshNI GGYRCC 1 cut(s) 326
BsiHKAI GWGCWC 3 cut(s) 33, 58, 294
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 240
BsnI GGCC 1 cut(s) 336
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 33, 58, 294
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 4 cut(s) 252, 590, 654, 703
BspACI CCGC 3 cut(s) 396, 454, 713
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 241
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 2 cut(s) 94, 328
BspPI GGATC 1 cut(s) 662
BspT104I TTCGAA 1 cut(s) 268
BspT107I GGYRCC 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 713
BsrDI GCAATG 1 cut(s) 132
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 151, 216, 243
BssMI GATC 4 cut(s) 252, 590, 654, 703
BssT1I CCWWGG 1 cut(s) 243
Bst4CI ACNGT 3 cut(s) 179, 626, 697
Bst6I CTCTTC 1 cut(s) 65
BstAUI TGTACA 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 268
BstDEI CTNAG 3 cut(s) 249, 632, 689
BstEII GGTNACC 1 cut(s) 680
BstF5I GGATG 2 cut(s) 53, 550
BstFNI CGCG 1 cut(s) 396
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 4 cut(s) 255, 593, 657, 706
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 4 cut(s) 252, 590, 654, 703
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstNSI RCATGY 3 cut(s) 265, 479, 649
BstPI GGTNACC 1 cut(s) 680
BstSCI CCNGG 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 625
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 336
BtsCI GGATG 2 cut(s) 53, 550
CfoI GCGC 1 cut(s) 437
Csp6I GTAC 2 cut(s) 16, 316
CviAII CATG 6 cut(s) 262, 457, 476, 646, 668, 707
CviJI RGCY 8 cut(s) 56, 116, 221, 248, 292, 336, 559, 721
CviKI_1 RGCY 8 cut(s) 56, 116, 221, 248, 292, 336, 559, 721
CviQI GTAC 2 cut(s) 16, 316
DdeI CTNAG 3 cut(s) 249, 632, 689
DpnI GATC 4 cut(s) 254, 592, 656, 705
DpnII GATC 4 cut(s) 252, 590, 654, 703
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Ecl136II GAGCTC 2 cut(s) 56, 292
Eco130I CCWWGG 1 cut(s) 243
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 2 cut(s) 58, 294
Eco53kI GAGCTC 2 cut(s) 56, 292
Eco88I CYCGRG 1 cut(s) 293
Eco91I GGTNACC 1 cut(s) 680
EcoICRI GAGCTC 2 cut(s) 56, 292
EcoO65I GGTNACC 1 cut(s) 680
EcoT14I CCWWGG 1 cut(s) 243
EcoT22I ATGCAT 1 cut(s) 669
EcoT38I GRGCYC 2 cut(s) 58, 294
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 6 cut(s) 265, 460, 479, 649, 671, 710
FatI CATG 6 cut(s) 261, 456, 475, 645, 667, 706
FauI CCCGC 1 cut(s) 389
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 2 cut(s) 60, 557
FriOI GRGCYC 2 cut(s) 58, 294
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 436
GluI GCNGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 17
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 1 cut(s) 336
HapII CCGG 1 cut(s) 151
HhaI GCGC 1 cut(s) 437
Hin1II CATG 6 cut(s) 265, 460, 479, 649, 671, 710
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 2 cut(s) 259, 733
HindII GTYRAC 2 cut(s) 259, 733
HinfI GANTC 2 cut(s) 73, 233
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 1 cut(s) 151
HphI GGTGA 4 cut(s) 205, 581, 692, 727
Hpy166II GTNNAC 3 cut(s) 259, 629, 733
Hpy188I TCNGA 3 cut(s) 504, 659, 728
Hpy188III TCNNGA 4 cut(s) 158, 295, 415, 701
Hpy8I GTNNAC 3 cut(s) 259, 629, 733
HpyAV CCTTC 2 cut(s) 15, 106
HpyCH4III ACNGT 3 cut(s) 179, 626, 697
HpyCH4IV ACGT 2 cut(s) 205, 386
HpyCH4V TGCA 2 cut(s) 667, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 3 cut(s) 249, 632, 689
HpySE526I ACGT 2 cut(s) 205, 386
Hsp92II CATG 6 cut(s) 265, 460, 479, 649, 671, 710
HspAI GCGC 1 cut(s) 435
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 4 cut(s) 252, 590, 654, 703
LmnI GCTCC 3 cut(s) 36, 53, 710
LpnPI CCDG 4 cut(s) 47, 164, 479, 762
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 2 cut(s) 151, 654
MaeI CTAG 1 cut(s) 12
MaeII ACGT 2 cut(s) 205, 386
MaeIII GTNAC 2 cut(s) 211, 680
MalI GATC 4 cut(s) 254, 592, 656, 705
MbiI CCGCTC 1 cut(s) 713
MboI GATC 4 cut(s) 252, 590, 654, 703
MboII GAAGA 5 cut(s) 82, 119, 122, 565, 622
MhlI GDGCHC 3 cut(s) 33, 58, 294
MluCI AATT 2 cut(s) 311, 536
MlyI GAGTC 2 cut(s) 82, 242
Mph1103I ATGCAT 1 cut(s) 669
MroXI GAANNNNTTC 2 cut(s) 274, 614
MseI TTAA 2 cut(s) 377, 732
MslI CAYNNNNRTG 1 cut(s) 279
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 1 cut(s) 152
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 361
NciI CCSGG 1 cut(s) 152
NdeII GATC 4 cut(s) 252, 590, 654, 703
NlaIII CATG 6 cut(s) 265, 460, 479, 649, 671, 710
NlaIV GGNNCC 2 cut(s) 94, 328
NmeAIII GCCGAG 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 211, 680
NsiI ATGCAT 1 cut(s) 669
NspI RCATGY 3 cut(s) 265, 479, 649
NspV TTCGAA 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 293
PceI AGGCCT 1 cut(s) 336
PciI ACATGT 1 cut(s) 645
PdmI GAANNNNTTC 2 cut(s) 274, 614
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 81, 241
PpsI GAGTC 2 cut(s) 81, 241
PscI ACATGT 1 cut(s) 645
Psp124BI GAGCTC 2 cut(s) 58, 294
PspEI GGTNACC 1 cut(s) 680
PspN4I GGNNCC 2 cut(s) 94, 328
RsaI GTAC 2 cut(s) 17, 317
RsaNI GTAC 2 cut(s) 16, 316
RseI CAYNNNNRTG 1 cut(s) 279
SacI GAGCTC 2 cut(s) 58, 294
SaqAI TTAA 2 cut(s) 377, 732
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 4 cut(s) 252, 590, 654, 703
SchI GAGTC 2 cut(s) 82, 242
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 3 cut(s) 33, 58, 294
SfaNI GCATC 2 cut(s) 151, 654
SfcI CTRYAG 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 2 cut(s) 311, 536
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 3 cut(s) 396, 454, 713
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 2 cut(s) 58, 294
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 3 cut(s) 179, 626, 697
TaiI ACGT 2 cut(s) 208, 389
TaqI TCGA 5 cut(s) 181, 268, 294, 414, 642
TasI AATT 2 cut(s) 311, 536
TatI WGTACW 1 cut(s) 315
Tru1I TTAA 2 cut(s) 377, 732
Tru9I TTAA 2 cut(s) 377, 732
TseFI GTSAC 2 cut(s) 211, 680
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 2 cut(s) 211, 680
TspDTI ATGAA 5 cut(s) 120, 206, 374, 488, 607
TspGWI ACGGA 1 cut(s) 757
XapI RAATTY 1 cut(s) 536
XceI RCATGY 3 cut(s) 265, 479, 649
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 628
XmnI GAANNNNTTC 2 cut(s) 274, 614
XspI CTAG 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.