MD09G1074100.v1.1

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002429
Physical Location & Seq
Forward (+)
5150027 .. 5152374
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1074100.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGTGAGGATTCTGGATCGATACGACCAATATTCCTATGCAGAACGGCAACGCACCGGAGCTGATTCTGAATCACAGGAAAACTGGCCGGTGGAATACCCCAAGCTTGCGGCAAGGATTGAAGTCTTACAAAGGAAACTAAGGAATTTGGTGGGAGAAGATTTAGACCCCTTAAGCTTGAGAGAGCTTCAAGATTTGGAGCAACAGCTTGATACAGCTCTTAAGCGCATACGAACAAGAAAGAACCAACTCGTGCATGAATCCATTTCAGAGATGGACAAGAAGCGAAAGGCTCTACGGGAACTAAACAAGTCGCTAGCAAAGAAGGTGAAGGAGAATAAAACGATGCTTGAGGAAGAACATGACCAGGTGCAGTTAGTAGGGCAGCAGCAAACTAACCAAGGCCGCCACAACTCATCCACCCTCATGCTCATGCCGCCGCCGCCCCAACCCCCATCAACACCAACACTACCTACTTTTCGAACCACCAGTGGGGGAGTCCAGGCAAGAGGAGCAGTGGATGACAATGATTCTGATAAAGGCGGAAGACCTCCACCTCGACCGGCTGCTGCTACAAACACACGCATGCCACTGTGGATGCTTTCGCCATTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

206

Amino Acids

23.45

Weight (kDa)

9.12

Isoelectric Point (pI)

59.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 23 - 110 7.2e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 110, 406, 437, 440, 443, 543
AclWI GGATC 1 cut(s) 24
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 492
AflII CTTAAG 2 cut(s) 172, 221
AgsI TTSAA 3 cut(s) 122, 191, 614
AjnI CCWGG 2 cut(s) 366, 501
AluBI AGCT 6 cut(s) 62, 106, 177, 187, 208, 218
AluI AGCT 6 cut(s) 62, 106, 177, 187, 208, 218
AlwI GGATC 1 cut(s) 24
AoxI GGCC 2 cut(s) 86, 403
ApeKI GCWGC 4 cut(s) 385, 388, 566, 569
ApoI RAATTY 1 cut(s) 145
AspLEI GCGC 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 16, 340
AsuII TTCGAA 1 cut(s) 481
AsuNHI GCTAGC 1 cut(s) 316
BauI CACGAG 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 553
BbvI GCAGC 4 cut(s) 397, 400, 553, 556
BccI CCATC 2 cut(s) 268, 463
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 2 cut(s) 368, 503
BfaI CTAG 1 cut(s) 317
BfrI CTTAAG 2 cut(s) 172, 221
BisI GCNGC 9 cut(s) 111, 386, 389, 406, 437, 440, 443, 567, 570
BlsI GCNGC 9 cut(s) 112, 387, 390, 407, 438, 441, 444, 568, 571
Bme1390I CCNGG 2 cut(s) 368, 503
BmrFI CCNGG 2 cut(s) 368, 503
BmsI GCATC 2 cut(s) 336, 588
BmtI GCTAGC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 553
Bpu14I TTCGAA 1 cut(s) 481
BpuEI CTTGAG 2 cut(s) 199, 371
Bsa29I ATCGAT 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 400
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse118I RCCGGY 2 cut(s) 88, 562
Bse1I ACTGG 2 cut(s) 89, 489
BseBI CCWGG 2 cut(s) 368, 503
BseCI ATCGAT 1 cut(s) 19
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 3 cut(s) 416, 526, 603
BseLI CCNNNNNNNGG 1 cut(s) 492
BseNI ACTGG 2 cut(s) 89, 489
BseRI GAGGAG 1 cut(s) 525
BseXI GCAGC 4 cut(s) 397, 400, 553, 556
BsgI GTGCAG 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 563
BshFI GGCC 2 cut(s) 88, 405
BshVI ATCGAT 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 563
BsiSI CCGG 3 cut(s) 57, 89, 563
BslI CCNNNNNNNGG 1 cut(s) 492
BsnI GGCC 2 cut(s) 88, 405
Bsp119I TTCGAA 1 cut(s) 481
Bsp143I GATC 1 cut(s) 16
BspACI CCGC 6 cut(s) 110, 406, 437, 440, 443, 543
BspANI GGCC 2 cut(s) 88, 405
BspDI ATCGAT 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 24
BspT104I TTCGAA 1 cut(s) 481
BspTI CTTAAG 2 cut(s) 172, 221
BsrFI RCCGGY 2 cut(s) 88, 562
BsrI ACTGG 2 cut(s) 89, 489
BssAI RCCGGY 2 cut(s) 88, 562
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 1 cut(s) 16
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 400
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 2 cut(s) 368, 503
Bst4CI ACNGT 1 cut(s) 594
BstAFI CTTAAG 2 cut(s) 172, 221
BstBI TTCGAA 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 108, 318, 587
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 416, 526, 603
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstMCI CGRYCG 1 cut(s) 563
BstMWI GCNNNNNNNGC 3 cut(s) 436, 442, 512
BstNI CCWGG 2 cut(s) 368, 503
BstNSI RCATGY 1 cut(s) 589
BstSCI CCNGG 2 cut(s) 366, 501
BstV1I GCAGC 4 cut(s) 397, 400, 553, 556
BstV2I GAAGAC 1 cut(s) 553
Bsu15I ATCGAT 1 cut(s) 19
BsuRI GGCC 2 cut(s) 88, 405
BsuTUI ATCGAT 1 cut(s) 19
BtsCI GGATG 3 cut(s) 416, 526, 603
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 3 cut(s) 496, 522, 590
Cac8I GCNNGC 3 cut(s) 108, 318, 587
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 2 cut(s) 88, 562
ClaI ATCGAT 1 cut(s) 19
CsiI ACCWGGT 1 cut(s) 366
CviAII CATG 5 cut(s) 257, 362, 427, 433, 586
DdeI CTNAG 1 cut(s) 140
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 86
EciI GGCGGA 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 400
EcoRII CCWGG 2 cut(s) 366, 501
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 5 cut(s) 260, 365, 430, 436, 589
FaiI YATR 7 cut(s) 39, 230, 258, 363, 428, 434, 587
FatI CATG 5 cut(s) 256, 361, 426, 432, 585
Fnu4HI GCNGC 9 cut(s) 111, 386, 389, 406, 437, 440, 443, 567, 570
FokI GGATG 3 cut(s) 403, 533, 610
Fsp4HI GCNGC 9 cut(s) 111, 386, 389, 406, 437, 440, 443, 567, 570
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 227
GluI GCNGC 9 cut(s) 111, 386, 389, 406, 437, 440, 443, 567, 570
HaeIII GGCC 2 cut(s) 88, 405
HapII CCGG 3 cut(s) 57, 89, 563
HhaI GCGC 1 cut(s) 228
Hin1II CATG 5 cut(s) 260, 365, 430, 436, 589
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HindIII AAGCTT 2 cut(s) 104, 175
HinfI GANTC 6 cut(s) 10, 65, 71, 260, 498, 530
HpaII CCGG 3 cut(s) 57, 89, 563
HphI GGTGA 2 cut(s) 16, 340
Hpy188I TCNGA 3 cut(s) 70, 271, 535
Hpy188III TCNNGA 2 cut(s) 14, 191
HpyAV CCTTC 2 cut(s) 319, 325
HpyCH4III ACNGT 1 cut(s) 594
HpyCH4V TGCA 3 cut(s) 41, 256, 373
HpyF10VI GCNNNNNNNGC 3 cut(s) 436, 442, 512
HpyF3I CTNAG 1 cut(s) 140
Hsp92II CATG 5 cut(s) 260, 365, 430, 436, 589
HspAI GCGC 1 cut(s) 226
Kzo9I GATC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 59, 199, 512
Lsp1109I GCAGC 4 cut(s) 397, 400, 553, 556
LweI GCATC 2 cut(s) 336, 588
MabI ACCWGGT 1 cut(s) 366
MaeI CTAG 1 cut(s) 317
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 3 cut(s) 170, 368, 558
MluCI AATT 1 cut(s) 145
MlyI GAGTC 1 cut(s) 507
MnlI CCTC 5 cut(s) 346, 434, 503, 561, 567
MseI TTAA 2 cut(s) 173, 222
MslI CAYNNNNRTG 4 cut(s) 425, 431, 584, 613
MspCI CTTAAG 2 cut(s) 172, 221
MspI CCGG 3 cut(s) 57, 89, 563
MspR9I CCNGG 2 cut(s) 368, 503
MvaI CCWGG 2 cut(s) 368, 503
MwoI GCNNNNNNNGC 3 cut(s) 436, 442, 512
NdeII GATC 1 cut(s) 16
NheI GCTAGC 1 cut(s) 316
NlaIII CATG 5 cut(s) 260, 365, 430, 436, 589
NspI RCATGY 1 cut(s) 589
NspV TTCGAA 1 cut(s) 481
PaeI GCATGC 1 cut(s) 589
PfeI GAWTC 5 cut(s) 10, 65, 71, 260, 530
PkrI GCNGC 9 cut(s) 112, 387, 390, 407, 438, 441, 444, 568, 571
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Psp6I CCWGG 2 cut(s) 366, 501
PspGI CCWGG 2 cut(s) 366, 501
RseI CAYNNNNRTG 4 cut(s) 425, 431, 584, 613
SaqAI TTAA 2 cut(s) 173, 222
SatI GCNGC 9 cut(s) 111, 386, 389, 406, 437, 440, 443, 567, 570
Sau3AI GATC 1 cut(s) 16
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 2 cut(s) 368, 503
SexAI ACCWGGT 1 cut(s) 366
SfaNI GCATC 2 cut(s) 336, 588
SfuI TTCGAA 1 cut(s) 481
SmiMI CAYNNNNRTG 4 cut(s) 425, 431, 584, 613
SmlI CTYRAG 4 cut(s) 172, 178, 221, 350
SmoI CTYRAG 4 cut(s) 172, 178, 221, 350
SphI GCATGC 1 cut(s) 589
Sse9I AATT 1 cut(s) 145
SsiI CCGC 6 cut(s) 110, 406, 437, 440, 443, 543
SspI AATATT 1 cut(s) 32
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 2 cut(s) 366, 501
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 1 cut(s) 594
TaqI TCGA 3 cut(s) 19, 481, 559
TasI AATT 1 cut(s) 145
TauI GCSGC 5 cut(s) 113, 408, 439, 442, 445
TfiI GAWTC 5 cut(s) 10, 65, 71, 260, 530
Tru1I TTAA 2 cut(s) 173, 222
Tru9I TTAA 2 cut(s) 173, 222
TscAI CASTG 3 cut(s) 496, 522, 597
TseI GCWGC 4 cut(s) 385, 388, 566, 569
TspDTI ATGAA 1 cut(s) 273
TspRI CASTG 3 cut(s) 496, 522, 597
Vha464I CTTAAG 2 cut(s) 172, 221
XapI RAATTY 1 cut(s) 145
XceI RCATGY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.