RLG00000021499

K-box region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002429
Physical Location & Seq
Reverse (-)
75919693 .. 75924134
4442 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021499

Sequence Viewer

Length: 792 bp
ATGGGAAGAGGCAAGGTTCAGCTGAAGCGGATCGAGAACAAGATCAGCAGACAAGTAACATTCTCGAAGCGAAGGACAGGATTGCTCAAGAAAGCTCATGAGATCTCAGTTCTGTGTGATGCTGAGGTAGCTTTGATTGTCTTCTCCACCAAAGGCAAGCTCTTTGAGTACGCTACCGATTCCAGCATGGAGGGGATCCTGGAGCGATATGAACAATACTCGTATGCAGAAAGGCAGAGCATGGGTGTTCCTGCTTCTGAATCACAGGGAAACTGGTCCATGGAATTTCCCAAGCTTGCGGCAAGGATTGAAATCTTACAAAGGAAAATAAGGAATTACACGGGAGAGGATTTAGACCCCTTAAGCTTGAGGGAGCTTCAAAGTTTGGAGCAACAGATTGATACAGCTCTTAAGCGTGTGCGAGCAAGAAAGAACCAAGTAATGCATGAATCCATTTCAGAGATGCAGAAAAAGCACAGGACACTGCAAGAGCAAAACAACTCGCTAGCAAAGAAGCTGAAGGAGAATGAAAAGCTGCTGCAGGAAGAACCTAATAATAATCAGCAGCCAAACCCCTCAACCCTGGTCTTAATGCCGCCACCGCAAGCTACATCACCACCAGCCACATCACCACCAGCCCTACTGTCTTCTCTAACTATTGGTGGGGCTTTCCAGGGAAGAGGAGACGCAATGGATGAAGATGCTGAGGATCATCAGGGAAGAGCTCATCAAACTCGGCCTGCCAGTAACACACTCATGCCACCATGGATGGTTCGCCATTTGAATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

264

Amino Acids

29.8

Weight (kDa)

9.34

Isoelectric Point (pI)

63.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.7e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 87 - 173 8.7e-29 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 28, 299, 596, 602
AclWI GGATC 4 cut(s) 38, 190, 203, 717
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 2 cut(s) 44, 539
AfaI GTAC 1 cut(s) 170
AflII CTTAAG 2 cut(s) 361, 410
AgsI TTSAA 3 cut(s) 311, 380, 784
AjnI CCWGG 3 cut(s) 198, 582, 672
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 1 cut(s) 678
AlwI GGATC 4 cut(s) 38, 190, 203, 717
AoxI GGCC 1 cut(s) 737
ApeKI GCWGC 3 cut(s) 535, 538, 565
ApoI RAATTY 1 cut(s) 284
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 2 cut(s) 606, 621
AsuNHI GCTAGC 1 cut(s) 505
AvaII GGWCC 1 cut(s) 276
BamHI GGATCC 1 cut(s) 195
BanII GRGCYC 1 cut(s) 727
BbsI GAAGAC 2 cut(s) 133, 639
Bbv12I GWGCWC 1 cut(s) 727
BbvCI CCTCAGC 2 cut(s) 123, 705
BbvI GCAGC 3 cut(s) 522, 525, 577
BccI CCATC 1 cut(s) 763
BciT130I CCWGG 3 cut(s) 200, 584, 674
BcoDI GTCTC 1 cut(s) 678
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 539
BfrI CTTAAG 2 cut(s) 361, 410
BglII AGATCT 1 cut(s) 102
BisI GCNGC 5 cut(s) 300, 536, 539, 566, 596
BlsI GCNGC 5 cut(s) 301, 537, 540, 567, 597
Bme1390I CCNGG 3 cut(s) 200, 584, 674
Bme18I GGWCC 1 cut(s) 276
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 3 cut(s) 200, 584, 674
BmsI GCATC 3 cut(s) 109, 453, 691
BmtI GCTAGC 1 cut(s) 509
BpiI GAAGAC 2 cut(s) 133, 639
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 123, 705
BpuEI CTTGAG 2 cut(s) 71, 388
BsaBI GATNNNNATC 1 cut(s) 311
BsaJI CCNNGG 4 cut(s) 279, 582, 673, 764
Bse1I ACTGG 2 cut(s) 278, 744
Bse3DI GCAATG 1 cut(s) 696
Bse8I GATNNNNATC 1 cut(s) 311
BseBI CCWGG 3 cut(s) 200, 584, 674
BseDI CCNNGG 4 cut(s) 279, 582, 673, 764
BseGI GGATG 2 cut(s) 700, 774
BseJI GATNNNNATC 1 cut(s) 311
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 3 cut(s) 114, 120, 696
BseNI ACTGG 2 cut(s) 278, 744
BseRI GAGGAG 1 cut(s) 696
BseXI GCAGC 3 cut(s) 522, 525, 577
BshFI GGCC 1 cut(s) 739
BsiHKAI GWGCWC 1 cut(s) 727
BsmAI GTCTC 1 cut(s) 678
BsmBI CGTCTC 1 cut(s) 678
BsnI GGCC 1 cut(s) 739
Bsp1286I GDGCHC 1 cut(s) 727
Bsp143I GATC 5 cut(s) 30, 42, 102, 195, 709
Bsp19I CCATGG 2 cut(s) 279, 764
BspACI CCGC 4 cut(s) 28, 299, 596, 602
BspANI GGCC 1 cut(s) 739
BspCNI CTCAG 3 cut(s) 115, 119, 697
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 197
BspMAI CTGCAG 1 cut(s) 543
BspOI GCTAGC 1 cut(s) 509
BspPI GGATC 4 cut(s) 38, 190, 203, 717
BspQI GCTCTTC 1 cut(s) 715
BspTI CTTAAG 2 cut(s) 361, 410
BsrDI GCAATG 1 cut(s) 696
BsrI ACTGG 2 cut(s) 278, 744
BssECI CCNNGG 4 cut(s) 279, 582, 673, 764
BssMI GATC 5 cut(s) 30, 42, 102, 195, 709
BssT1I CCWWGG 2 cut(s) 279, 764
Bst2UI CCWGG 3 cut(s) 200, 584, 674
Bst4CI ACNGT 1 cut(s) 645
Bst6I CTCTTC 2 cut(s) 673, 715
BstAFI CTTAAG 2 cut(s) 361, 410
BstC8I GCNNGC 6 cut(s) 158, 297, 423, 507, 606, 741
BstDEI CTNAG 3 cut(s) 106, 123, 705
BstDSI CCRYGG 2 cut(s) 279, 764
BstF5I GGATG 2 cut(s) 700, 774
BstKTI GATC 5 cut(s) 33, 45, 105, 198, 712
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 5 cut(s) 30, 42, 102, 195, 709
BstMWI GCNNNNNNNGC 3 cut(s) 128, 472, 601
BstNI CCWGG 3 cut(s) 200, 584, 674
BstSCI CCNGG 3 cut(s) 198, 582, 672
BstSFI CTRYAG 1 cut(s) 539
BstV1I GCAGC 3 cut(s) 522, 525, 577
BstV2I GAAGAC 2 cut(s) 133, 639
BstX2I RGATCY 2 cut(s) 102, 195
BstYI RGATCY 2 cut(s) 102, 195
BsuRI GGCC 1 cut(s) 739
BtgI CCRYGG 2 cut(s) 279, 764
BtsCI GGATG 2 cut(s) 700, 774
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 6 cut(s) 158, 297, 423, 507, 606, 741
CciI TCATGA 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 276
CseI GACGC 1 cut(s) 695
Csp6I GTAC 1 cut(s) 169
CviAII CATG 7 cut(s) 98, 187, 241, 280, 446, 757, 765
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 3 cut(s) 106, 123, 705
DpnI GATC 5 cut(s) 32, 44, 104, 197, 711
DpnII GATC 5 cut(s) 30, 42, 102, 195, 709
Eam1104I CTCTTC 2 cut(s) 673, 715
EarI CTCTTC 2 cut(s) 673, 715
Ecl136II GAGCTC 1 cut(s) 725
Eco130I CCWWGG 2 cut(s) 279, 764
Eco24I GRGCYC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 725
Eco57I CTGAAG 2 cut(s) 44, 539
EcoICRI GAGCTC 1 cut(s) 725
EcoRII CCWGG 3 cut(s) 198, 582, 672
EcoT14I CCWWGG 2 cut(s) 279, 764
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 727
ErhI CCWWGG 2 cut(s) 279, 764
Esp3I CGTCTC 1 cut(s) 678
FaeI CATG 7 cut(s) 101, 190, 244, 283, 449, 760, 768
FaiI YATR 9 cut(s) 99, 188, 210, 225, 242, 281, 447, 758, 766
FatI CATG 7 cut(s) 97, 186, 240, 279, 445, 756, 764
Fnu4HI GCNGC 5 cut(s) 300, 536, 539, 566, 596
FokI GGATG 2 cut(s) 707, 781
FriOI GRGCYC 1 cut(s) 727
Fsp4HI GCNGC 5 cut(s) 300, 536, 539, 566, 596
FspBI CTAG 1 cut(s) 506
GluI GCNGC 5 cut(s) 300, 536, 539, 566, 596
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 739
HgaI GACGC 1 cut(s) 695
Hin1II CATG 7 cut(s) 101, 190, 244, 283, 449, 760, 768
HindIII AAGCTT 2 cut(s) 293, 364
HinfI GANTC 3 cut(s) 179, 260, 449
HphI GGTGA 2 cut(s) 606, 621
Hpy188I TCNGA 2 cut(s) 259, 460
Hpy188III TCNNGA 4 cut(s) 34, 64, 88, 98
HpyAV CCTTC 2 cut(s) 66, 514
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4V TGCA 5 cut(s) 227, 445, 466, 487, 541
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 472, 601
HpyF3I CTNAG 3 cut(s) 106, 123, 705
Hsp92II CATG 7 cut(s) 101, 190, 244, 283, 449, 760, 768
Kzo9I GATC 5 cut(s) 30, 42, 102, 195, 709
LguI GCTCTTC 1 cut(s) 715
LmnI GCTCC 3 cut(s) 202, 373, 388
Lsp1109I GCAGC 3 cut(s) 522, 525, 577
LweI GCATC 3 cut(s) 109, 453, 691
MaeI CTAG 1 cut(s) 506
MaeIII GTNAC 2 cut(s) 55, 746
MalI GATC 5 cut(s) 32, 44, 104, 197, 711
MboI GATC 5 cut(s) 30, 42, 102, 195, 709
MboII GAAGA 7 cut(s) 18, 133, 557, 639, 690, 710, 732
MflI RGATCY 2 cut(s) 102, 195
MhlI GDGCHC 1 cut(s) 727
MluCI AATT 2 cut(s) 284, 334
MnlI CCTC 7 cut(s) 118, 184, 340, 363, 586, 674, 700
Mph1103I ATGCAT 1 cut(s) 447
MseI TTAA 3 cut(s) 362, 411, 590
MslI CAYNNNNRTG 1 cut(s) 755
MspA1I CMGCKG 1 cut(s) 22
MspCI CTTAAG 2 cut(s) 361, 410
MspR9I CCNGG 3 cut(s) 200, 584, 674
MvaI CCWGG 3 cut(s) 200, 584, 674
MwoI GCNNNNNNNGC 3 cut(s) 128, 472, 601
NcoI CCATGG 2 cut(s) 279, 764
NdeII GATC 5 cut(s) 30, 42, 102, 195, 709
NheI GCTAGC 1 cut(s) 505
NlaIII CATG 7 cut(s) 101, 190, 244, 283, 449, 760, 768
NlaIV GGNNCC 1 cut(s) 197
NmeAIII GCCGAG 1 cut(s) 715
NsiI ATGCAT 1 cut(s) 447
PagI TCATGA 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 715
PfeI GAWTC 3 cut(s) 179, 260, 449
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 5 cut(s) 301, 537, 540, 567, 597
Psp124BI GAGCTC 1 cut(s) 727
Psp6I CCWGG 3 cut(s) 198, 582, 672
PspGI CCWGG 3 cut(s) 198, 582, 672
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 1 cut(s) 276
PstI CTGCAG 1 cut(s) 543
PsuI RGATCY 2 cut(s) 102, 195
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 1 cut(s) 755
SacI GAGCTC 1 cut(s) 727
SapI GCTCTTC 1 cut(s) 715
SaqAI TTAA 3 cut(s) 362, 411, 590
SatI GCNGC 5 cut(s) 300, 536, 539, 566, 596
Sau3AI GATC 5 cut(s) 30, 42, 102, 195, 709
Sau96I GGNCC 1 cut(s) 276
ScrFI CCNGG 3 cut(s) 200, 584, 674
SduI GDGCHC 1 cut(s) 727
SfaNI GCATC 3 cut(s) 109, 453, 691
SfcI CTRYAG 1 cut(s) 539
SinI GGWCC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 755
SmlI CTYRAG 4 cut(s) 86, 361, 367, 410
SmoI CTYRAG 4 cut(s) 86, 361, 367, 410
Sse9I AATT 2 cut(s) 284, 334
SsiI CCGC 4 cut(s) 28, 299, 596, 602
SspMI CTAG 1 cut(s) 506
SstI GAGCTC 1 cut(s) 727
StyD4I CCNGG 3 cut(s) 198, 582, 672
StyI CCWWGG 2 cut(s) 279, 764
TaaI ACNGT 1 cut(s) 645
TaqI TCGA 2 cut(s) 33, 65
TasI AATT 2 cut(s) 284, 334
TauI GCSGC 2 cut(s) 302, 598
TfiI GAWTC 3 cut(s) 179, 260, 449
Tru1I TTAA 3 cut(s) 362, 411, 590
Tru9I TTAA 3 cut(s) 362, 411, 590
TscAI CASTG 1 cut(s) 489
TseI GCWGC 3 cut(s) 535, 538, 565
TspDTI ATGAA 4 cut(s) 225, 462, 543, 711
TspRI CASTG 1 cut(s) 489
Vha464I CTTAAG 2 cut(s) 361, 410
VpaK11BI GGWCC 1 cut(s) 276
XapI RAATTY 1 cut(s) 284
XspI CTAG 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.