Rh2CG561400

K-box region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002429
Physical Location & Seq
Reverse (-)
73971631 .. 73975057
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG561400.1

Sequence Viewer

Length: 600 bp
ATGGGATGCTTCATGGAGGGGATCCTGGAGCGATATGAACAATACTCGTATGCAGAAAGGCAGAGCATGGGCGTTCCTGCTTCTGAATCACAGGGAAACTGGTCCATGGAATTTCCCAAGCTTACGGCAAGGATTGAAATCTTACAAAGGAAAATAAGGAATTACACGGGAGAGGATTTAGACCCCTTAAGCTTGAGGGAGCTTCAAAGTTTGGAGCAACAGATTGATACAGCTCTTAAGCGCGTGCGAGCAAGAAAGAACCAAGTAATGCATGAATCCATTTCAGAGATGCAGAAAAAGCACAGGACACTGCAAGAGCAAAACAACACGCTAGCAAAGAAGCTGAAGGAGAATGAAAAGCTTCTGCAGGAAGAACCTAATAATAATCAGCAGCCAAACCCCTCAACCCTCGTCTTAATGCCACCACTGCAAGCTACATCACCACCAGCCCTACTCTCTTCTCTAACTATTGGTGGGGCTTTCCAGGGAAGAGGAGACGCAATGGATGAAGATGCTGAGGATCATCAGGGAAGCGCTCAAACTCGGCCTGCCAGTAACACACTCATGCCACCATGGATGGTTCGCCATTTGAATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

199

Amino Acids

22.67

Weight (kDa)

6.13

Isoelectric Point (pI)

64.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 29 - 115 3.5e-29 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 243
AclWI GGATC 3 cut(s) 16, 29, 528
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 365
AfeI AGCGCT 1 cut(s) 535
AflII CTTAAG 2 cut(s) 187, 236
AgsI TTSAA 3 cut(s) 137, 206, 592
AjnI CCWGG 2 cut(s) 24, 483
AluBI AGCT 7 cut(s) 121, 192, 202, 233, 343, 361, 434
AluI AGCT 7 cut(s) 121, 192, 202, 233, 343, 361, 434
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 3 cut(s) 16, 29, 528
Aor51HI AGCGCT 1 cut(s) 535
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 1 cut(s) 391
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 2 cut(s) 243, 536
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 432
AsuNHI GCTAGC 1 cut(s) 331
AvaII GGWCC 1 cut(s) 102
BamHI GGATCC 1 cut(s) 21
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 1 cut(s) 403
BccI CCATC 1 cut(s) 571
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 2 cut(s) 26, 485
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 365
BfoI RGCGCY 1 cut(s) 537
BfrI CTTAAG 2 cut(s) 187, 236
BisI GCNGC 1 cut(s) 392
BlsI GCNGC 1 cut(s) 393
Bme1390I CCNGG 2 cut(s) 26, 485
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 2 cut(s) 26, 485
BmsI GCATC 2 cut(s) 279, 502
BmtI GCTAGC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 47
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 137
BsaJI CCNNGG 3 cut(s) 105, 484, 572
Bse1I ACTGG 2 cut(s) 104, 552
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 26, 485
BseDI CCNNGG 3 cut(s) 105, 484, 572
BseGI GGATG 3 cut(s) 11, 511, 582
BseJI GATNNNNATC 1 cut(s) 137
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 2 cut(s) 104, 552
BseRI GAGGAG 1 cut(s) 507
BseXI GCAGC 1 cut(s) 403
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 1 cut(s) 547
BsmAI GTCTC 1 cut(s) 489
BsmBI CGTCTC 1 cut(s) 489
BsnI GGCC 1 cut(s) 547
Bsp143I GATC 2 cut(s) 21, 520
Bsp19I CCATGG 2 cut(s) 105, 572
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 23
BspMAI CTGCAG 1 cut(s) 369
BspOI GCTAGC 1 cut(s) 335
BspPI GGATC 3 cut(s) 16, 29, 528
BspTI CTTAAG 2 cut(s) 187, 236
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 104, 552
BssECI CCNNGG 3 cut(s) 105, 484, 572
BssMI GATC 2 cut(s) 21, 520
BssT1I CCWWGG 2 cut(s) 105, 572
Bst2UI CCWGG 2 cut(s) 26, 485
Bst6I CTCTTC 2 cut(s) 463, 484
BstAFI CTTAAG 2 cut(s) 187, 236
BstC8I GCNNGC 5 cut(s) 245, 249, 333, 432, 549
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 2 cut(s) 105, 572
BstF5I GGATG 3 cut(s) 11, 511, 582
BstFNI CGCG 1 cut(s) 243
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 2 cut(s) 243, 536
BstKTI GATC 2 cut(s) 24, 523
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 2 cut(s) 21, 520
BstMWI GCNNNNNNNGC 2 cut(s) 298, 427
BstNI CCWGG 2 cut(s) 26, 485
BstSCI CCNGG 2 cut(s) 24, 483
BstSFI CTRYAG 1 cut(s) 365
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 403
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BsuRI GGCC 1 cut(s) 547
BtgI CCRYGG 2 cut(s) 105, 572
BtsCI GGATG 3 cut(s) 11, 511, 582
BtsI GCAGTG 2 cut(s) 308, 425
BtsIMutI CAGTG 2 cut(s) 308, 425
Cac8I GCNNGC 5 cut(s) 245, 249, 333, 432, 549
CfoI GCGC 2 cut(s) 243, 536
Cfr13I GGNCC 1 cut(s) 102
CseI GACGC 1 cut(s) 506
CviAII CATG 6 cut(s) 13, 67, 106, 272, 565, 573
DdeI CTNAG 1 cut(s) 516
DpnI GATC 2 cut(s) 23, 522
DpnII GATC 2 cut(s) 21, 520
Eam1104I CTCTTC 2 cut(s) 463, 484
EarI CTCTTC 2 cut(s) 463, 484
Eco130I CCWWGG 2 cut(s) 105, 572
Eco47I GGWCC 1 cut(s) 102
Eco47III AGCGCT 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 365
EcoRII CCWGG 2 cut(s) 24, 483
EcoT14I CCWWGG 2 cut(s) 105, 572
EcoT22I ATGCAT 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 105, 572
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 6 cut(s) 16, 70, 109, 275, 568, 576
FaiI YATR 8 cut(s) 14, 36, 51, 68, 107, 273, 566, 574
FatI CATG 6 cut(s) 12, 66, 105, 271, 564, 572
Fnu4HI GCNGC 1 cut(s) 392
FokI GGATG 3 cut(s) 18, 518, 589
Fsp4HI GCNGC 1 cut(s) 392
FspBI CTAG 1 cut(s) 332
GlaI GCGC 2 cut(s) 242, 535
GluI GCNGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 1 cut(s) 547
HgaI GACGC 1 cut(s) 506
HhaI GCGC 2 cut(s) 243, 536
Hin1II CATG 6 cut(s) 16, 70, 109, 275, 568, 576
Hin6I GCGC 2 cut(s) 241, 534
HinP1I GCGC 2 cut(s) 241, 534
HindIII AAGCTT 3 cut(s) 119, 190, 359
HinfI GANTC 2 cut(s) 86, 275
HphI GGTGA 1 cut(s) 432
Hpy188I TCNGA 2 cut(s) 85, 286
HpyAV CCTTC 1 cut(s) 340
HpyCH4V TGCA 6 cut(s) 53, 271, 292, 313, 367, 430
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 427
HpyF3I CTNAG 1 cut(s) 516
Hsp92II CATG 6 cut(s) 16, 70, 109, 275, 568, 576
HspAI GCGC 2 cut(s) 241, 534
Kzo9I GATC 2 cut(s) 21, 520
LmnI GCTCC 3 cut(s) 28, 199, 214
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 2 cut(s) 279, 502
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 554
MalI GATC 2 cut(s) 23, 522
MboI GATC 2 cut(s) 21, 520
MboII GAAGA 4 cut(s) 383, 450, 501, 521
MflI RGATCY 1 cut(s) 21
MluCI AATT 2 cut(s) 110, 160
MnlI CCTC 7 cut(s) 10, 166, 189, 412, 419, 485, 511
Mph1103I ATGCAT 1 cut(s) 273
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 188, 237, 416
MslI CAYNNNNRTG 1 cut(s) 563
MspCI CTTAAG 2 cut(s) 187, 236
MspR9I CCNGG 2 cut(s) 26, 485
MvaI CCWGG 2 cut(s) 26, 485
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 2 cut(s) 298, 427
NcoI CCATGG 2 cut(s) 105, 572
NdeII GATC 2 cut(s) 21, 520
NheI GCTAGC 1 cut(s) 331
NlaIII CATG 6 cut(s) 16, 70, 109, 275, 568, 576
NlaIV GGNNCC 1 cut(s) 23
NmeAIII GCCGAG 1 cut(s) 523
NsiI ATGCAT 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 86, 275
PfoI TCCNGGA 1 cut(s) 24
PkrI GCNGC 1 cut(s) 393
Psp6I CCWGG 2 cut(s) 24, 483
PspGI CCWGG 2 cut(s) 24, 483
PspN4I GGNNCC 1 cut(s) 23
PspPI GGNCC 1 cut(s) 102
PstI CTGCAG 1 cut(s) 369
PsuI RGATCY 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 563
SaqAI TTAA 3 cut(s) 188, 237, 416
SatI GCNGC 1 cut(s) 392
Sau3AI GATC 2 cut(s) 21, 520
Sau96I GGNCC 1 cut(s) 102
ScrFI CCNGG 2 cut(s) 26, 485
SetI ASST 8 cut(s) 123, 194, 204, 235, 345, 363, 379, 436
SfaNI GCATC 2 cut(s) 279, 502
SfcI CTRYAG 1 cut(s) 365
SinI GGWCC 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 3 cut(s) 187, 193, 236
SmoI CTYRAG 3 cut(s) 187, 193, 236
Sse9I AATT 2 cut(s) 110, 160
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 24, 483
StyI CCWWGG 2 cut(s) 105, 572
TasI AATT 2 cut(s) 110, 160
TfiI GAWTC 2 cut(s) 86, 275
Tru1I TTAA 3 cut(s) 188, 237, 416
Tru9I TTAA 3 cut(s) 188, 237, 416
TscAI CASTG 2 cut(s) 315, 432
TseI GCWGC 1 cut(s) 391
TspDTI ATGAA 4 cut(s) 51, 288, 369, 522
TspRI CASTG 2 cut(s) 315, 432
Vha464I CTTAAG 2 cut(s) 187, 236
VpaK11BI GGWCC 1 cut(s) 102
XapI RAATTY 1 cut(s) 110
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 332
Zsp2I ATGCAT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.