MD17G1065500.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002429
Physical Location & Seq
Forward (+)
5300195 .. 5307702
7508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1065500.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGGAAGAGGTAAGGTTCAGCTGAAACGAATCGAGAACACGATAAGCAGGCAAGTGACATTCTCAAAGAGGAGGACCGGATTGCTCAAAAAAGCTCATGAGATCTCTGTTCTGTGTGATGCTGATGTGGCACTTATTGTCTTCTCCACCAAAGGGAAGCTCTTTGAGTATTCTACTGATTCCAGCATGGAGAGGATTCTGGATCGATACGAACAATATACCGTTGCAGAACGGCAACTAAACGGAACTAATTCTGAATCACAGGAAAACTGGTGTGTGGAATACCCCAAACTTGCGGCAAGGATTGAAGTCATACAAAGGAAGCTGAGGAATTTTACGGGAGAAGATTTAGGACCCTTAAGCTTGAGAGAGCTTCAAAATTTGGAGCAACAGCTTGATACAGCTCTTAAGCGCATAAGAACAAGAAAGAACCAACTCATGCATGAATCCATTTCAGAGATGCACAAGAAGCAAAAGGCACTACGGGAACTAAACAACTCGCTAGCAAAGCAGGTCAAGGAGAATGGAAAGATGCTTGAGGAAGAGCATGATCAGGTGCAGGTAGTAGGGCGGCAGCAGCAAACTAACCAAGGCCGCCACAACTCATCCACCCTCATGCTAATGCCGCCACCCCAACCCCCATCGACACCATCACTACCTACTTCTCGAAGCACCAGTGGGGGATTCCAGGCAAGAGGAGCAACGGACGGTGATTACGAGGGAAGACCTCGGCCACCTGCTGCTAAAAACACACACATGCCACTGTGGATGCTTTCGCCATTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

263

Amino Acids

29.84

Weight (kDa)

9.66

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 4.9e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 85 - 172 2.8e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 745
Acc36I ACCTGC 3 cut(s) 502, 550, 745
AciI CCGC 4 cut(s) 296, 571, 595, 626
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 731
AcsI RAATTY 2 cut(s) 331, 379
AfiI CCNNNNNNNGG 1 cut(s) 153
AflII CTTAAG 2 cut(s) 358, 407
AgsI TTSAA 2 cut(s) 308, 377
AjnI CCWGG 1 cut(s) 687
AluBI AGCT 8 cut(s) 22, 95, 160, 325, 363, 373, 394, 404
AluI AGCT 8 cut(s) 22, 95, 160, 325, 363, 373, 394, 404
AlwI GGATC 1 cut(s) 210
AoxI GGCC 2 cut(s) 592, 731
ApeKI GCWGC 3 cut(s) 574, 577, 740
ApoI RAATTY 2 cut(s) 331, 379
Asp700I GAANNNNTTC 1 cut(s) 250
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 75, 353
AsuHPI GGTGA 1 cut(s) 722
AsuNHI GCTAGC 1 cut(s) 502
AvaII GGWCC 2 cut(s) 75, 353
BbsI GAAGAC 2 cut(s) 133, 730
BbvCI CCTCAGC 1 cut(s) 326
BbvI GCAGC 3 cut(s) 586, 589, 727
BccI CCATC 2 cut(s) 649, 658
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 689
BclI TGATCA 1 cut(s) 550
BfaI CTAG 1 cut(s) 503
BfrI CTTAAG 2 cut(s) 358, 407
BfuAI ACCTGC 3 cut(s) 502, 550, 745
BglII AGATCT 1 cut(s) 102
BisI GCNGC 7 cut(s) 297, 572, 575, 578, 595, 626, 741
BlsI GCNGC 7 cut(s) 298, 573, 576, 579, 596, 627, 742
Bme1390I CCNGG 1 cut(s) 689
Bme18I GGWCC 2 cut(s) 75, 353
BmgT120I GGNCC 2 cut(s) 75, 353
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 689
BmsI GCATC 4 cut(s) 109, 450, 522, 759
BmtI GCTAGC 1 cut(s) 506
BpiI GAAGAC 2 cut(s) 133, 730
Bpu10I CCTNAGC 1 cut(s) 326
BpuEI CTTGAG 2 cut(s) 385, 557
Bsa29I ATCGAT 1 cut(s) 205
BsaJI CCNNGG 2 cut(s) 589, 728
BsaWI WCCGGW 1 cut(s) 77
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse1I ACTGG 2 cut(s) 275, 675
BseBI CCWGG 1 cut(s) 689
BseCI ATCGAT 1 cut(s) 205
BseDI CCNNGG 2 cut(s) 589, 728
BseGI GGATG 2 cut(s) 605, 774
BseLI CCNNNNNNNGG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 275, 675
BseRI GAGGAG 2 cut(s) 85, 711
BseXI GCAGC 3 cut(s) 586, 589, 727
BsgI GTGCAG 1 cut(s) 578
BshFI GGCC 2 cut(s) 594, 733
BshVI ATCGAT 1 cut(s) 205
BsiSI CCGG 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 153
BsnI GGCC 2 cut(s) 594, 733
Bsp143I GATC 3 cut(s) 102, 202, 550
BspACI CCGC 4 cut(s) 296, 571, 595, 626
BspANI GGCC 2 cut(s) 594, 733
BspCNI CTCAG 1 cut(s) 318
BspDI ATCGAT 1 cut(s) 205
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 355
BspMI ACCTGC 3 cut(s) 502, 550, 745
BspOI GCTAGC 1 cut(s) 506
BspPI GGATC 1 cut(s) 210
BspQI GCTCTTC 1 cut(s) 537
BspTI CTTAAG 2 cut(s) 358, 407
BsrI ACTGG 2 cut(s) 275, 675
BssECI CCNNGG 2 cut(s) 589, 728
BssMI GATC 3 cut(s) 102, 202, 550
BssT1I CCWWGG 1 cut(s) 589
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 3 cut(s) 223, 710, 765
Bst6I CTCTTC 1 cut(s) 537
BstAFI CTTAAG 2 cut(s) 358, 407
BstC8I GCNNGC 2 cut(s) 50, 504
BstDEI CTNAG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 605, 774
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 3 cut(s) 105, 205, 553
BstMBI GATC 3 cut(s) 102, 202, 550
BstMWI GCNNNNNNNGC 6 cut(s) 128, 469, 508, 577, 625, 698
BstNI CCWGG 1 cut(s) 689
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 1 cut(s) 687
BstV1I GCAGC 3 cut(s) 586, 589, 727
BstV2I GAAGAC 2 cut(s) 133, 730
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 205
BsuRI GGCC 2 cut(s) 594, 733
BsuTUI ATCGAT 1 cut(s) 205
BtsCI GGATG 2 cut(s) 605, 774
BtsIMutI CAGTG 2 cut(s) 682, 761
BveI ACCTGC 3 cut(s) 502, 550, 745
Cac8I GCNNGC 2 cut(s) 50, 504
CciI TCATGA 1 cut(s) 97
CfoI GCGC 1 cut(s) 414
Cfr13I GGNCC 2 cut(s) 75, 353
ClaI ATCGAT 1 cut(s) 205
CviAII CATG 7 cut(s) 98, 187, 439, 443, 548, 616, 757
DdeI CTNAG 1 cut(s) 326
DpnI GATC 3 cut(s) 104, 204, 552
DpnII GATC 3 cut(s) 102, 202, 550
EaeI YGGCCR 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 589
Eco47I GGWCC 2 cut(s) 75, 353
EcoO109I RGGNCCY 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 687
EcoT14I CCWWGG 1 cut(s) 589
EcoT22I ATGCAT 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 589
FaeI CATG 7 cut(s) 101, 190, 442, 446, 551, 619, 760
FatI CATG 7 cut(s) 97, 186, 438, 442, 547, 615, 756
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 7 cut(s) 297, 572, 575, 578, 595, 626, 741
FokI GGATG 2 cut(s) 592, 781
Fsp4HI GCNGC 7 cut(s) 297, 572, 575, 578, 595, 626, 741
FspBI CTAG 1 cut(s) 503
GlaI GCGC 1 cut(s) 413
GluI GCNGC 7 cut(s) 297, 572, 575, 578, 595, 626, 741
HaeIII GGCC 2 cut(s) 594, 733
HapII CCGG 1 cut(s) 78
HhaI GCGC 1 cut(s) 414
Hin1II CATG 7 cut(s) 101, 190, 442, 446, 551, 619, 760
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 6 cut(s) 30, 179, 196, 257, 446, 684
HpaII CCGG 1 cut(s) 78
HphI GGTGA 1 cut(s) 722
Hpy188I TCNGA 2 cut(s) 256, 457
Hpy188III TCNNGA 4 cut(s) 34, 98, 200, 666
HpyCH4III ACNGT 3 cut(s) 223, 710, 765
HpyCH4V TGCA 4 cut(s) 227, 442, 463, 559
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 469, 508, 577, 625, 698
HpyF3I CTNAG 1 cut(s) 326
Hsp92II CATG 7 cut(s) 101, 190, 442, 446, 551, 619, 760
HspAI GCGC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 3 cut(s) 102, 202, 550
LguI GCTCTTC 1 cut(s) 537
LmnI GCTCC 2 cut(s) 385, 698
Lsp1109I GCAGC 3 cut(s) 586, 589, 727
LweI GCATC 4 cut(s) 109, 450, 522, 759
MaeI CTAG 1 cut(s) 503
MaeIII GTNAC 1 cut(s) 55
MalI GATC 3 cut(s) 104, 204, 552
MboI GATC 3 cut(s) 102, 202, 550
MboII GAAGA 5 cut(s) 18, 133, 356, 554, 735
MflI RGATCY 1 cut(s) 102
MluCI AATT 3 cut(s) 250, 331, 379
MnlI CCTC 9 cut(s) 63, 66, 186, 321, 532, 623, 689, 712, 738
Mph1103I ATGCAT 1 cut(s) 444
MroXI GAANNNNTTC 1 cut(s) 250
MseI TTAA 2 cut(s) 359, 408
MslI CAYNNNNRTG 4 cut(s) 614, 620, 755, 784
MspA1I CMGCKG 1 cut(s) 22
MspCI CTTAAG 2 cut(s) 358, 407
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 1 cut(s) 689
MvaI CCWGG 1 cut(s) 689
MwoI GCNNNNNNNGC 6 cut(s) 128, 469, 508, 577, 625, 698
NdeII GATC 3 cut(s) 102, 202, 550
NheI GCTAGC 1 cut(s) 502
NlaIII CATG 7 cut(s) 101, 190, 442, 446, 551, 619, 760
NlaIV GGNNCC 1 cut(s) 355
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 444
NspI RCATGY 1 cut(s) 760
PagI TCATGA 1 cut(s) 97
PaqCI CACCTGC 1 cut(s) 745
PciSI GCTCTTC 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 714
PdmI GAANNNNTTC 1 cut(s) 250
PfeI GAWTC 6 cut(s) 30, 179, 196, 257, 446, 684
PkrI GCNGC 7 cut(s) 298, 573, 576, 579, 596, 627, 742
PpuMI RGGWCCY 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 687
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 1 cut(s) 355
PspPI GGNCC 2 cut(s) 75, 353
PspPPI RGGWCCY 1 cut(s) 353
PsuI RGATCY 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 22
RseI CAYNNNNRTG 4 cut(s) 614, 620, 755, 784
SapI GCTCTTC 1 cut(s) 537
SaqAI TTAA 2 cut(s) 359, 408
SatI GCNGC 7 cut(s) 297, 572, 575, 578, 595, 626, 741
Sau3AI GATC 3 cut(s) 102, 202, 550
Sau96I GGNCC 2 cut(s) 75, 353
ScrFI CCNGG 1 cut(s) 689
SfaNI GCATC 4 cut(s) 109, 450, 522, 759
SinI GGWCC 2 cut(s) 75, 353
SmiMI CAYNNNNRTG 4 cut(s) 614, 620, 755, 784
SmlI CTYRAG 4 cut(s) 358, 364, 407, 536
SmoI CTYRAG 4 cut(s) 358, 364, 407, 536
Sse9I AATT 3 cut(s) 250, 331, 379
SsiI CCGC 4 cut(s) 296, 571, 595, 626
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 1 cut(s) 687
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 3 cut(s) 223, 710, 765
TaqI TCGA 4 cut(s) 33, 205, 644, 667
TasI AATT 3 cut(s) 250, 331, 379
TauI GCSGC 4 cut(s) 299, 574, 597, 628
TfiI GAWTC 6 cut(s) 30, 179, 196, 257, 446, 684
Tru1I TTAA 2 cut(s) 359, 408
Tru9I TTAA 2 cut(s) 359, 408
TscAI CASTG 2 cut(s) 682, 768
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 3 cut(s) 574, 577, 740
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 1 cut(s) 459
TspGWI ACGGA 2 cut(s) 258, 719
TspRI CASTG 2 cut(s) 682, 768
Vha464I CTTAAG 2 cut(s) 358, 407
VpaK11BI GGWCC 2 cut(s) 75, 353
XapI RAATTY 2 cut(s) 331, 379
XceI RCATGY 1 cut(s) 760
XmnI GAANNNNTTC 1 cut(s) 250
XspI CTAG 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.