Rroxscaffold_2G00086190

K-box region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002429
Physical Location & Seq
Forward (+)
8441198 .. 8446522
5325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086190.1

Sequence Viewer

Length: 774 bp
ATGGGAAGAGGCAAGGTTCAGCTGAAGCGGATCGAGAACAAGATCAGCAGACAAGTAACATTCTCGAAGCGAAGGACAGGATTGCTCAAGAAAGCTCATGAGATCTCAGTTCTGTGTGATGCTGAGGTAGCTTTGATTGTCTTCTCCACCAAAGGCAAGCTCTTTGAGTACGCTACCGATTCCAGCATGGAGGGGATCCTGGAGCGATATGAACAATACTCGTATGCAGAAAGGCAGAGCATGGGTGTTCCTGCTTCTGAATCACAGGGAAACTGGTCCATGGAATTTCCCAAGCTTGCGGCAAGGATTGAAATCTTACAAAGGAAAATAAGGAATTACACGGGAGAGGATTTAGACCCCTTAAGCTTGAGGGAGCTTCAGAGTTTGGAGCAACAGATTGATACAGCTCTTAAGCGCGTGCGAGCAAGAAAGAACCAAGTAATGCATGAATCCATTTCAGAGATGCAGAAAAAGCACAGGACACTGCAAGAGCAAAACAACTCGCTAGCAAAGAAGCTGAAGGAGAATGAAAAGCTGCTGCAGGAAGAACCTAATAATAATCAGCAGCCAAACCCCTCAACCCTCGTCTTAATGCCGCCACCGCAAGCTACATCACCACCAGCCCTACTCTCTTCTCTAACTATTGGTGGGGCTTTCCAGGGAAGAGGAGACGCAATGGATGAAGATGCTGAGGATCATCAGGGAAGAGCTCAAACTCGGCCTGCCAGTAACACACTCATGCCACCATGGATGGTTCGCCATTTGAATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

257

Amino Acids

29.21

Weight (kDa)

9.34

Isoelectric Point (pI)

61.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.7e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 87 - 173 8.2e-29 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 417
AciI CCGC 4 cut(s) 28, 299, 596, 602
AclWI GGATC 4 cut(s) 38, 190, 203, 702
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 3 cut(s) 44, 362, 539
AfaI GTAC 1 cut(s) 170
AflII CTTAAG 2 cut(s) 361, 410
AgsI TTSAA 2 cut(s) 311, 766
AjnI CCWGG 2 cut(s) 198, 657
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 4 cut(s) 38, 190, 203, 702
AoxI GGCC 1 cut(s) 719
ApeKI GCWGC 3 cut(s) 535, 538, 565
ApoI RAATTY 1 cut(s) 284
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 606
AsuNHI GCTAGC 1 cut(s) 505
AvaII GGWCC 1 cut(s) 276
BamHI GGATCC 1 cut(s) 195
BanII GRGCYC 1 cut(s) 712
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 712
BbvCI CCTCAGC 2 cut(s) 123, 690
BbvI GCAGC 3 cut(s) 522, 525, 577
BccI CCATC 1 cut(s) 745
BciT130I CCWGG 2 cut(s) 200, 659
BcoDI GTCTC 1 cut(s) 663
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 539
BfrI CTTAAG 2 cut(s) 361, 410
BglII AGATCT 1 cut(s) 102
BisI GCNGC 5 cut(s) 300, 536, 539, 566, 596
BlsI GCNGC 5 cut(s) 301, 537, 540, 567, 597
Bme1390I CCNGG 2 cut(s) 200, 659
Bme18I GGWCC 1 cut(s) 276
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 2 cut(s) 200, 659
BmsI GCATC 3 cut(s) 109, 453, 676
BmtI GCTAGC 1 cut(s) 509
BpiI GAAGAC 1 cut(s) 133
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 123, 690
BpuEI CTTGAG 2 cut(s) 71, 388
BsaBI GATNNNNATC 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 279, 658, 746
Bse1I ACTGG 2 cut(s) 278, 726
Bse3DI GCAATG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 311
BseBI CCWGG 2 cut(s) 200, 659
BseDI CCNNGG 3 cut(s) 279, 658, 746
BseGI GGATG 2 cut(s) 685, 756
BseJI GATNNNNATC 1 cut(s) 311
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 3 cut(s) 114, 120, 681
BseNI ACTGG 2 cut(s) 278, 726
BseRI GAGGAG 1 cut(s) 681
BseXI GCAGC 3 cut(s) 522, 525, 577
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 721
BsiHKAI GWGCWC 1 cut(s) 712
BsmAI GTCTC 1 cut(s) 663
BsmBI CGTCTC 1 cut(s) 663
BsnI GGCC 1 cut(s) 721
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 5 cut(s) 30, 42, 102, 195, 694
Bsp19I CCATGG 2 cut(s) 279, 746
BspACI CCGC 4 cut(s) 28, 299, 596, 602
BspANI GGCC 1 cut(s) 721
BspCNI CTCAG 3 cut(s) 115, 119, 682
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 197
BspMAI CTGCAG 1 cut(s) 543
BspOI GCTAGC 1 cut(s) 509
BspPI GGATC 4 cut(s) 38, 190, 203, 702
BspQI GCTCTTC 1 cut(s) 700
BspTI CTTAAG 2 cut(s) 361, 410
BsrDI GCAATG 1 cut(s) 681
BsrI ACTGG 2 cut(s) 278, 726
BssECI CCNNGG 3 cut(s) 279, 658, 746
BssMI GATC 5 cut(s) 30, 42, 102, 195, 694
BssT1I CCWWGG 2 cut(s) 279, 746
Bst2UI CCWGG 2 cut(s) 200, 659
Bst6I CTCTTC 3 cut(s) 637, 658, 700
BstAFI CTTAAG 2 cut(s) 361, 410
BstC8I GCNNGC 7 cut(s) 158, 297, 419, 423, 507, 606, 723
BstDEI CTNAG 3 cut(s) 106, 123, 690
BstDSI CCRYGG 2 cut(s) 279, 746
BstF5I GGATG 2 cut(s) 685, 756
BstFNI CGCG 1 cut(s) 417
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 5 cut(s) 33, 45, 105, 198, 697
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 5 cut(s) 30, 42, 102, 195, 694
BstMWI GCNNNNNNNGC 3 cut(s) 128, 472, 601
BstNI CCWGG 2 cut(s) 200, 659
BstSCI CCNGG 2 cut(s) 198, 657
BstSFI CTRYAG 1 cut(s) 539
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 522, 525, 577
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 2 cut(s) 102, 195
BstYI RGATCY 2 cut(s) 102, 195
BsuRI GGCC 1 cut(s) 721
BtgI CCRYGG 2 cut(s) 279, 746
BtsCI GGATG 2 cut(s) 685, 756
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 7 cut(s) 158, 297, 419, 423, 507, 606, 723
CciI TCATGA 1 cut(s) 97
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 1 cut(s) 276
CseI GACGC 1 cut(s) 680
Csp6I GTAC 1 cut(s) 169
CviAII CATG 7 cut(s) 98, 187, 241, 280, 446, 739, 747
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 3 cut(s) 106, 123, 690
DpnI GATC 5 cut(s) 32, 44, 104, 197, 696
DpnII GATC 5 cut(s) 30, 42, 102, 195, 694
Eam1104I CTCTTC 3 cut(s) 637, 658, 700
EarI CTCTTC 3 cut(s) 637, 658, 700
Ecl136II GAGCTC 1 cut(s) 710
Eco130I CCWWGG 2 cut(s) 279, 746
Eco24I GRGCYC 1 cut(s) 712
Eco47I GGWCC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 710
Eco57I CTGAAG 3 cut(s) 44, 362, 539
EcoICRI GAGCTC 1 cut(s) 710
EcoRII CCWGG 2 cut(s) 198, 657
EcoT14I CCWWGG 2 cut(s) 279, 746
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 2 cut(s) 279, 746
Esp3I CGTCTC 1 cut(s) 663
FaeI CATG 7 cut(s) 101, 190, 244, 283, 449, 742, 750
FaiI YATR 9 cut(s) 99, 188, 210, 225, 242, 281, 447, 740, 748
FatI CATG 7 cut(s) 97, 186, 240, 279, 445, 738, 746
Fnu4HI GCNGC 5 cut(s) 300, 536, 539, 566, 596
FokI GGATG 2 cut(s) 692, 763
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 5 cut(s) 300, 536, 539, 566, 596
FspBI CTAG 1 cut(s) 506
GlaI GCGC 1 cut(s) 416
GluI GCNGC 5 cut(s) 300, 536, 539, 566, 596
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 721
HgaI GACGC 1 cut(s) 680
HhaI GCGC 1 cut(s) 417
Hin1II CATG 7 cut(s) 101, 190, 244, 283, 449, 742, 750
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HindIII AAGCTT 2 cut(s) 293, 364
HinfI GANTC 3 cut(s) 179, 260, 449
HphI GGTGA 1 cut(s) 606
Hpy188I TCNGA 3 cut(s) 259, 381, 460
Hpy188III TCNNGA 4 cut(s) 34, 64, 88, 98
HpyAV CCTTC 2 cut(s) 66, 514
HpyCH4V TGCA 5 cut(s) 227, 445, 466, 487, 541
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 472, 601
HpyF3I CTNAG 3 cut(s) 106, 123, 690
Hsp92II CATG 7 cut(s) 101, 190, 244, 283, 449, 742, 750
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 5 cut(s) 30, 42, 102, 195, 694
LguI GCTCTTC 1 cut(s) 700
LmnI GCTCC 3 cut(s) 202, 373, 388
Lsp1109I GCAGC 3 cut(s) 522, 525, 577
LweI GCATC 3 cut(s) 109, 453, 676
MaeI CTAG 1 cut(s) 506
MaeIII GTNAC 2 cut(s) 55, 728
MalI GATC 5 cut(s) 32, 44, 104, 197, 696
MboI GATC 5 cut(s) 30, 42, 102, 195, 694
MboII GAAGA 7 cut(s) 18, 133, 557, 624, 675, 695, 717
MflI RGATCY 2 cut(s) 102, 195
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 2 cut(s) 284, 334
MnlI CCTC 8 cut(s) 118, 184, 340, 363, 586, 593, 659, 685
Mph1103I ATGCAT 1 cut(s) 447
MseI TTAA 3 cut(s) 362, 411, 590
MslI CAYNNNNRTG 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 22
MspCI CTTAAG 2 cut(s) 361, 410
MspR9I CCNGG 2 cut(s) 200, 659
MvaI CCWGG 2 cut(s) 200, 659
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 3 cut(s) 128, 472, 601
NcoI CCATGG 2 cut(s) 279, 746
NdeII GATC 5 cut(s) 30, 42, 102, 195, 694
NheI GCTAGC 1 cut(s) 505
NlaIII CATG 7 cut(s) 101, 190, 244, 283, 449, 742, 750
NlaIV GGNNCC 1 cut(s) 197
NmeAIII GCCGAG 1 cut(s) 697
NsiI ATGCAT 1 cut(s) 447
PagI TCATGA 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 700
PfeI GAWTC 3 cut(s) 179, 260, 449
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 5 cut(s) 301, 537, 540, 567, 597
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 2 cut(s) 198, 657
PspGI CCWGG 2 cut(s) 198, 657
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 1 cut(s) 276
PstI CTGCAG 1 cut(s) 543
PsuI RGATCY 2 cut(s) 102, 195
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 712
SapI GCTCTTC 1 cut(s) 700
SaqAI TTAA 3 cut(s) 362, 411, 590
SatI GCNGC 5 cut(s) 300, 536, 539, 566, 596
Sau3AI GATC 5 cut(s) 30, 42, 102, 195, 694
Sau96I GGNCC 1 cut(s) 276
ScrFI CCNGG 2 cut(s) 200, 659
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 3 cut(s) 109, 453, 676
SfcI CTRYAG 1 cut(s) 539
SinI GGWCC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 4 cut(s) 86, 361, 367, 410
SmoI CTYRAG 4 cut(s) 86, 361, 367, 410
Sse9I AATT 2 cut(s) 284, 334
SsiI CCGC 4 cut(s) 28, 299, 596, 602
SspMI CTAG 1 cut(s) 506
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 2 cut(s) 198, 657
StyI CCWWGG 2 cut(s) 279, 746
TaqI TCGA 2 cut(s) 33, 65
TasI AATT 2 cut(s) 284, 334
TauI GCSGC 2 cut(s) 302, 598
TfiI GAWTC 3 cut(s) 179, 260, 449
Tru1I TTAA 3 cut(s) 362, 411, 590
Tru9I TTAA 3 cut(s) 362, 411, 590
TscAI CASTG 1 cut(s) 489
TseI GCWGC 3 cut(s) 535, 538, 565
TspDTI ATGAA 4 cut(s) 225, 462, 543, 696
TspRI CASTG 1 cut(s) 489
Vha464I CTTAAG 2 cut(s) 361, 410
VpaK11BI GGWCC 1 cut(s) 276
XapI RAATTY 1 cut(s) 284
XspI CTAG 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.