Rw4G031440

Floral homeotic protein APETALA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002429
Physical Location & Seq
Reverse (-)
58485380 .. 58487584
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031440.1

Sequence Viewer

Length: 606 bp
ATGGTAAACTTAAAATTAAACCAAAAGATATGTAACAACTCAGTATTCATGGAGAATATACTTGAACGCTATGAGCGGTACTCATACGCAGAGAGACAGCTAGTCGAACCTGATTTGGAATCACAGGGTAACTGGACCTTCGAACATGCTAGACTGAAGGTGAAAGTTGAGCTTCTGCAAAGAAACCTTAGGCACTATTTGGGAGAAGATCTGGATTCATTGAGTATCAAAGAGATCCAAAGTTTGGAGCAACAGCTTGACAATTCTCTTAAGCAAATTCGATCAAGAAAGAACCAACTCATGCATGAGTCTATGACCGAGCTTCAGAGAAAGGAGAAGGCAATGCAGGAGCAAAATAACTTGCTTTCAAAGAAGATTAAGGAGAAAGAGAAGAATGTAGCGGAAGCTCAGGAGGTTCATAATTGGGAGCAGCAGCAACTGCAGCAAAACCATGGCCTTAACCTGGTAGCACAGGCTCCACTTCCATGTTTGAACATGGGGGGCACTCAGCAAAATGATCAGTTCCTTCAAGTGAGGAGGAACCAGCTCGACCTTACTCTTGAGCCATCGTTGTATTCATGCCACCTTGGATGCTTTCCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

201

Amino Acids

23.69

Weight (kDa)

6.32

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 41 - 127 2.2e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 76
AciI CCGC 2 cut(s) 76, 401
AclWI GGATC 1 cut(s) 229
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 2 cut(s) 176, 308
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 244, 463
AflII CTTAAG 1 cut(s) 269
AgsI TTSAA 4 cut(s) 65, 369, 493, 530
AhdI GACNNNNNGTC 1 cut(s) 101
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 6 cut(s) 100, 172, 256, 322, 407, 547
AluI AGCT 6 cut(s) 100, 172, 256, 322, 407, 547
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 1 cut(s) 229
AlwNI CAGNNNCTG 1 cut(s) 439
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 3 cut(s) 430, 433, 442
ApoI RAATTY 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 135
AsuHPI GGTGA 1 cut(s) 172
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 1 cut(s) 135
AxyI CCTNAGG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 506
BbvI GCAGC 3 cut(s) 442, 445, 454
BccI CCATC 1 cut(s) 574
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 2 cut(s) 101, 150
BfmI CTRYAG 1 cut(s) 440
BfrI CTTAAG 1 cut(s) 269
BglII AGATCT 1 cut(s) 208
BisI GCNGC 3 cut(s) 431, 434, 443
BlsI GCNGC 3 cut(s) 432, 435, 444
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 135
BmeRI GACNNNNNGTC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 2 cut(s) 477, 542
BmrFI CCNGG 1 cut(s) 464
BmsI GCATC 1 cut(s) 581
BplI GAGNNNNNCTC 2 cut(s) 65, 97
Bpu10I CCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 581
BsaJI CCNNGG 2 cut(s) 451, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 463
Bse1I ACTGG 1 cut(s) 137
Bse21I CCTNAGG 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 348
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 2 cut(s) 451, 586
BseGI GGATG 1 cut(s) 596
BseLI CCNNNNNNNGG 2 cut(s) 244, 463
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 3 cut(s) 54, 422, 521
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 1 cut(s) 550
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 3 cut(s) 442, 445, 454
BshFI GGCC 1 cut(s) 456
BslI CCNNNNNNNGG 2 cut(s) 244, 463
BsmAI GTCTC 1 cut(s) 88
BsnI GGCC 1 cut(s) 456
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 4 cut(s) 208, 234, 281, 517
Bsp19I CCATGG 1 cut(s) 451
BspACI CCGC 2 cut(s) 76, 401
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 3 cut(s) 53, 421, 520
BspHI TCATGA 1 cut(s) 602
BspLI GGNNCC 2 cut(s) 477, 542
BspMAI CTGCAG 1 cut(s) 444
BspPI GGATC 1 cut(s) 229
BspT104I TTCGAA 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 269
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 348
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 451, 586
BssMI GATC 4 cut(s) 208, 234, 281, 517
BssT1I CCWWGG 2 cut(s) 451, 586
Bst2UI CCWGG 1 cut(s) 464
BstAFI CTTAAG 1 cut(s) 269
BstAPI GCANNNNNTGC 1 cut(s) 439
BstBI TTCGAA 1 cut(s) 141
BstDEI CTNAG 4 cut(s) 40, 188, 408, 507
BstDSI CCRYGG 1 cut(s) 451
BstF5I GGATG 1 cut(s) 596
BstKTI GATC 4 cut(s) 211, 237, 284, 520
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 4 cut(s) 208, 234, 281, 517
BstMWI GCNNNNNNNGC 2 cut(s) 439, 442
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 462
BstSFI CTRYAG 1 cut(s) 440
BstSLI GKGCMC 1 cut(s) 506
BstV1I GCAGC 3 cut(s) 442, 445, 454
BstX2I RGATCY 2 cut(s) 208, 234
BstYI RGATCY 2 cut(s) 208, 234
Bsu36I CCTNAGG 1 cut(s) 188
BsuRI GGCC 1 cut(s) 456
BtgI CCRYGG 1 cut(s) 451
BtsCI GGATG 1 cut(s) 596
CaiI CAGNNNCTG 1 cut(s) 439
CciI TCATGA 1 cut(s) 602
Cfr13I GGNCC 1 cut(s) 135
CsiI ACCWGGT 1 cut(s) 462
Csp6I GTAC 1 cut(s) 79
CviAII CATG 9 cut(s) 49, 146, 301, 305, 452, 486, 496, 579, 603
CviJI RGCY 9 cut(s) 100, 172, 256, 322, 407, 456, 476, 547, 565
CviKI_1 RGCY 9 cut(s) 100, 172, 256, 322, 407, 456, 476, 547, 565
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 4 cut(s) 40, 188, 408, 507
DpnI GATC 4 cut(s) 210, 236, 283, 519
DpnII GATC 4 cut(s) 208, 234, 281, 517
DriI GACNNNNNGTC 1 cut(s) 101
Eam1105I GACNNNNNGTC 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 451, 586
Eco47I GGWCC 1 cut(s) 135
Eco57I CTGAAG 2 cut(s) 176, 308
Eco81I CCTNAGG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 2 cut(s) 451, 586
EcoT22I ATGCAT 1 cut(s) 306
ErhI CCWWGG 2 cut(s) 451, 586
FaeI CATG 9 cut(s) 52, 149, 304, 308, 455, 489, 499, 582, 606
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 9 cut(s) 48, 145, 300, 304, 451, 485, 495, 578, 602
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 3 cut(s) 431, 434, 443
Fsp4HI GCNGC 3 cut(s) 431, 434, 443
FspBI CTAG 2 cut(s) 101, 150
GluI GCNGC 3 cut(s) 431, 434, 443
HaeIII GGCC 1 cut(s) 456
Hin1II CATG 9 cut(s) 52, 149, 304, 308, 455, 489, 499, 582, 606
HinfI GANTC 3 cut(s) 119, 215, 308
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 5 cut(s) 212, 285, 410, 560, 603
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 4 cut(s) 148, 151, 331, 536
HpyCH4V TGCA 4 cut(s) 178, 304, 346, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 439, 442
HpyF3I CTNAG 4 cut(s) 40, 188, 408, 507
Hsp92II CATG 9 cut(s) 52, 149, 304, 308, 455, 489, 499, 582, 606
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 4 cut(s) 208, 234, 281, 517
LmnI GCTCC 4 cut(s) 247, 349, 427, 481
Lsp1109I GCAGC 3 cut(s) 442, 445, 454
LweI GCATC 1 cut(s) 581
MabI ACCWGGT 1 cut(s) 462
MaeI CTAG 2 cut(s) 101, 150
MaeIII GTNAC 2 cut(s) 32, 128
MalI GATC 4 cut(s) 210, 236, 283, 519
MbiI CCGCTC 1 cut(s) 76
MboI GATC 4 cut(s) 208, 234, 281, 517
MboII GAAGA 3 cut(s) 218, 385, 403
MflI RGATCY 2 cut(s) 208, 234
MhlI GDGCHC 1 cut(s) 506
MluCI AATT 4 cut(s) 14, 262, 276, 421
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 3 cut(s) 406, 528, 531
Mph1103I ATGCAT 1 cut(s) 306
MseI TTAA 5 cut(s) 11, 17, 270, 378, 459
MslI CAYNNNNRTG 1 cut(s) 484
MspCI CTTAAG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 2 cut(s) 439, 442
NcoI CCATGG 1 cut(s) 451
NdeII GATC 4 cut(s) 208, 234, 281, 517
NlaIII CATG 9 cut(s) 52, 149, 304, 308, 455, 489, 499, 582, 606
NlaIV GGNNCC 2 cut(s) 477, 542
NsiI ATGCAT 1 cut(s) 306
NspI RCATGY 1 cut(s) 149
NspV TTCGAA 1 cut(s) 141
PagI TCATGA 1 cut(s) 602
PfeI GAWTC 2 cut(s) 119, 215
PflMI CCANNNNNTGG 1 cut(s) 244
PkrI GCNGC 3 cut(s) 432, 435, 444
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 2 cut(s) 477, 542
PspPI GGNCC 1 cut(s) 135
PstI CTGCAG 1 cut(s) 444
PstNI CAGNNNCTG 1 cut(s) 439
PsuI RGATCY 2 cut(s) 208, 234
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 484
SaqAI TTAA 5 cut(s) 11, 17, 270, 378, 459
SatI GCNGC 3 cut(s) 431, 434, 443
Sau3AI GATC 4 cut(s) 208, 234, 281, 517
Sau96I GGNCC 1 cut(s) 135
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 1 cut(s) 506
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 1 cut(s) 581
SfcI CTRYAG 1 cut(s) 440
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 1 cut(s) 135
SmiMI CAYNNNNRTG 1 cut(s) 484
SmlI CTYRAG 2 cut(s) 269, 560
SmoI CTYRAG 2 cut(s) 269, 560
Sse9I AATT 4 cut(s) 14, 262, 276, 421
SsiI CCGC 2 cut(s) 76, 401
SspMI CTAG 2 cut(s) 101, 150
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 2 cut(s) 451, 586
TaqI TCGA 4 cut(s) 105, 141, 280, 549
TaqII GACCGA 1 cut(s) 332
TasI AATT 4 cut(s) 14, 262, 276, 421
TfiI GAWTC 2 cut(s) 119, 215
Tru1I TTAA 5 cut(s) 11, 17, 270, 378, 459
Tru9I TTAA 5 cut(s) 11, 17, 270, 378, 459
TseI GCWGC 3 cut(s) 430, 433, 442
TspDTI ATGAA 5 cut(s) 37, 207, 407, 567, 591
Van91I CCANNNNNTGG 1 cut(s) 244
Vha464I CTTAAG 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 135
XapI RAATTY 1 cut(s) 276
XceI RCATGY 1 cut(s) 149
XspI CTAG 2 cut(s) 101, 150
Zsp2I ATGCAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.