Rorug04G0304400

Floral homeotic protein APETALA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002429
Physical Location & Seq
Forward (+)
47966676 .. 48001114
34439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0304400.1

Sequence Viewer

Length: 3906 bp
ATGGGTGATCAGGAGAATCCTTGGGATGGAGATCATGGAATCAGAGAGGAAGCTGCCGCGTCGAAAAGTCAATCAGCAGTGCCGGAATCCCAGAACGGACCGGAAAATAGTAGTAACAAGGAAGATGCAAGCAAGAGCAAGGAGGGTGGCACCAAGACAGTACCTTTTTTCAAGCTCTTCTCATTTGCTGATTCCATGGATTACTTGTTAATGTCAGTTGGTACGATTAGCGCAATTGGAAATGGAATGTGTTTGCCTCTAATGACCGTAATCATGGGAGATGTGATTAATTCTTTTGGAGAATCTGGGAATTCCAATGAAGTGGTTGATACAGTGTCTAAGGTGGCTTTAAAGTTCGTCTACTTGGCTTTGGGAGCTGCAGCTGCTTCATTTCTACAGATGTCTTGCTGGATGATCACCGGAGAGAGACAGGCTGCGAGAATAAGATTTTTATACTTGAAAACAATATTGAGGCAAGACGTAGGCTTTTTTGATAAAGAAACTAGCACTGGAGAAGTTATTGGGAGGATGTCAGGTGATACTGTGCTCATTCAGGAGGCCATGGGAGAGAAGGTAGGAACTTGTATCCAGTTATTTGCAACATTCTTTGGAGGCTTTATCATAGCCTTTGTTAAGGGATGGCTTCTTACCCTGGTCATGCTTTCTTCTATCCCCGCTCTTGTCATCTCTGGTGCTGTCATGAGCATCATCGTATCAAAGTTGGCATCTCGTGGACAAGCTGCTTATTCAGCAGCAGCATCTGTGGTTGAGCAGACAATTGGTTCAATAAGAACTGTTGCATCATTTACAGGGGAGAAACAAGCTATAACTAAGTACAATAGCTCTATAAATAAAGCTTACAAGTCTGGTGTGCAAGAGGGTTTGGCATCTGGGTTGGGTATCGGTTCAGTTACGCTTATTATATTCTGTAGTTATGCTTTGGCTATATGGTATGGCGGAAAAATGATACTTGAAAAGGGATATAAAGGAGGAGATGTCATAAATGTAGTTTTTGCTGTGTTGACTGGCTCCTTCTCTCTTGGGCAGACATCTCCATGCTTGGCCGCATTTGCTTCTGGGAAAGCTGCAGCTTATAAGATGTTTGAGACAATTAGCAGAAACCCAGAGATAGATGCTTCTGATACTAAAGGGAAGCAATTACAAGATATTAGAGGAGACATAGAACTGAGGGATGTTCATTTTAGTTATCCTGCAAGACCAGATGAACATATATTCCGTGGATTTTCTCTCACAATACCTAGTGGTGCAACTGCTGCTTTGGTAGGACAGAGCGGAAGTGGTAAATCGACTGTAATAAGTTTGATTGAGAGATTTTATGACCCACAGGCCGGTGAAGTTCTTATTGACGGTGTCAATCTCAAAGAGTTTCAGCTGAAATGGATCAGGAAGAAAATAGGTATTGTCAGCCAGGAACCTGTTTTGTTTACTTGCAGCATTAGAGAAAATATTGGCTATGGGAAGGATGGTGCAACTACTGAAGAGATAAGGGCTGCTGCTGAGCTTGCCAATGCTGCTAAATTCATAGACAAACTTCCTCAGGGACTAGATACAATGGTGGGTGAGCATGGAACTCAACTATCTGGGGGCCAAAAGCAGAGAGTTGCTATAGCTCGAGCAATTCTAAAAAACCCAAGAATTCTACTTCTAGATGAAGCCACAAGTGCTCTTGACGCAGAATCTGAGAGAGTTGTGCAGGAGGCACTGGATAGAATTATGGTTAACCGGACTACTGTCATAGTAGCCCATCGCTTGAGCACAGTAAGGAATGCTGATACCATTGCTGTCATACATCGAGGAACAATTGTTGAACAAGGACCACATTCCGAGCTAGTTAAGGATCCTGAGGGAGCATATAGCCAGCTTATAAGATTACAAGAAACCAGAACTGTGTCAGAACAGACTGGTGTAAATGATCAGGATAGGCCAGATATTTCTTCCGATTCTCGGAGACAGTCAAGTCAAAGATTTTCTTTATTAAGATCCATAAGCCGTGGATCATCTGGAAGAGCAAATAGTATTCGTCATTCATTCTCAATCTCATATGGTGGTGCACCTACTGCAATCGGCATCCTTGAAACAAATCCTGCTGAACCTGATACTCATGCTCCAGCATCATCAAAAGGGCATCCAGAAGTCTCACTTAGCCGCCTAGCTGCCCTGAACAAGCCAGAGATCCCAGTGCTGCTTCTTGGTACTTTAGCTGCAGCAGCCAATGGGGTGATCTTACCTGTTTTTGGGATACTAATATCCAGTGTAATCAAGACCTTCTTTGAGCCACCTGATCAACTGAGTAAGGATTCAAAGTTTTGGGCATTAATCTTTGTTGTTCTTGGAGTTGCATCATTCTTGGCACAATCATCAAGAGCATATCTTTTTGGTGTGGCTGGATGTAAGTTGATAAGAAGAGTTCGATCAAAGTGCTTTGAGAAGGTGGTTTACATGGACATAGGTTGGTTTGATGAGTCTGATCATTCAAGTGGTGCAATTGGGGCAAGGCTTTCTACAGATGCGGCTTCTCTAAGAGGGTTGGTTGGAGATGCTCTAGGTTTATTGGTTCAGAATTTAGCAACTGCAGTTGCCGGCCTAGTTATTGCTTTCGTAGCAAATTGGCAATTGGCTCTTATAATTCTTGTCCTGCTGCCTTTGTTAGGACTAAGTGGATATTTTCAAGTCAAGTTAATGAAAGGGTTCAGTGCAGATGCAAAGAAAATGTACGAGGACGCAAGCCAAGTAGCCAATGATGCTGTGGGGAGTATTAGAACAATTGCTTCCTTTTGTGCTGAAGAGAAGGTGATGGAACTGTACCAGAAAAAATGTGAAGGTCCTATTAAAAATGGGATAAGACAAGGCTTAGTTAGCGGAATTGGTTTTGGGCTATCGTTCTTTTTCCTATTTTCTGTGTATGCCTGCAGTTTCTACGCTGGAGCCCGACTTGTTGCAGCAGGCAAGACAGAATTTTCTGACGTGTTTCGGGTTTTCTTTGCTCTCACTATGACAGCTGTTGGAATATCTCAGTCGAGCTCCCTAGCCCCTGATATAAGTAAAGGAAAGAGTTCTGCTTCTTCCATATTTGCAATTCTTGACGGCAAGTCAAAAATAGACTCTAGTGACGACTCTGGAACTACAAGAGAAAATGTGAAGGGAGATATTGAACTACGCCATGTCAGCTTCAAGTATCCAACTAGACCCAACGTGCCAATCTTCCAGGATCTTTGCTTGACCATTCGTCATGGCAAGACGGTTGCTTTGGTTGGAGAAAGTGGTAGTGGGAAGTCGACAGTGATCTCATTGTTGCAGAGATTTTATGACCCTGATTCAGGTCACATTACATTAGATGGAATCGAAATCCAAAAGCTACAATTGAAGTGGTTGAGACAGCAAATGGGGCTGGTGAGCCAAGAGCCTCTGCTGTTTAATGACACTATCCGAGCCAACATTGCATATGGAAAGGATGGGAATGCAACCGAAGCTGAAATTATAGCTGCAGCAGAATTGGCAAATGCCCACAAGTTCATCAGTAGCTTACAACGGGGTTACGATACAGTAGTAGGAGAGCGGGGGGTCCAAATGTCAGGTGGACAGAAACAGAGGGTGGCAATTGCACGAGCTATTATGAAGGCGCCAAAGATATTACTCCTAGATGAGGCCACCAGTGCACTTGATGCTGAGTCTGAACGAGTCGTTCAAGACGCATTGGACAGAGTAATGGTGGATCGAACAACAGTGGTGGTTGCTCATCGGTTATCGACAATCAGAAGTGCAGATTTGATAGCAGTGGTAAAGAATGGGGTCATTGCAGAGAAAGGAAAGCATGAGACTTTGATTAATATCAAGGATGGCACTTATGCTTCTCTGGTAGCATTACATGCAAGTGCCTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

1301

Amino Acids

139.55

Weight (kDa)

8.04

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 73 - 345 6e-60 ABC transporter transmembrane region
ABC_tran PF00005 413 - 561 2.3e-34 ABC transporter
ABC_membrane PF00664 735 - 1008 4.8e-63 ABC transporter transmembrane region
ABC_tran PF00005 1076 - 1225 1.4e-34 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1095, 1886, 2645
AasI GACNNNNNNGTC 1 cut(s) 1977
AccB1I GGYRCC 2 cut(s) 149, 3640
AccBSI CCGCTC 3 cut(s) 677, 1293, 3577
AccI GTMKAC 2 cut(s) 360, 3296
AccII CGCG 1 cut(s) 59
AciI CCGC 9 cut(s) 57, 675, 957, 1065, 1293, 2167, 2531, 2880, 3577
AclWI GGATC 8 cut(s) 1409, 1853, 1866, 1995, 2023, 2188, 3237, 3741
AcoI YGGCCR 1 cut(s) 1062
AcsI RAATTY 5 cut(s) 310, 1538, 1656, 2581, 2975
AcuI CTGAAG 2 cut(s) 1518, 2823
AcyI GRCGYC 1 cut(s) 3641
AfaI GTAC 6 cut(s) 162, 223, 836, 2215, 2735, 2825
AfiI CCNNNNNNNGG 7 cut(s) 26, 1349, 1965, 2255, 2669, 3338, 3664
AflIII ACRYGT 1 cut(s) 2985
AhdI GACNNNNNGTC 2 cut(s) 3109, 3246
AjiI CACGTC 1 cut(s) 2986
AjnI CCWGG 3 cut(s) 651, 1428, 3225
AloI GAACNNNNNNTCC 2 cut(s) 1218, 1250
Alw21I GWGCWC 6 cut(s) 549, 1687, 1778, 2074, 3044, 3679
Alw26I GTCTC 7 cut(s) 421, 1100, 1170, 1963, 2161, 3388, 3830
Alw44I GTGCAC 2 cut(s) 2070, 3675
AlwI GGATC 8 cut(s) 1409, 1853, 1866, 1995, 2023, 2188, 3237, 3741
AlwNI CAGNNNCTG 2 cut(s) 761, 1700
Ama87I CYCGRG 1 cut(s) 1632
AoxI GGCC 7 cut(s) 558, 1062, 1347, 1606, 1943, 2602, 3666
ApaLI GTGCAC 2 cut(s) 2070, 3675
ApoI RAATTY 5 cut(s) 310, 1538, 1656, 2581, 2975
ArsI GACNNNNNNTTYG 2 cut(s) 3250, 3282
AseI ATTAAT 3 cut(s) 288, 2336, 3846
Asp700I GAANNNNTTC 2 cut(s) 2708, 3073
AspLEI GCGC 2 cut(s) 233, 3643
AspS9I GGNCC 5 cut(s) 98, 1606, 1835, 2843, 3583
AsuHPI GGTGA 8 cut(s) 17, 409, 548, 1364, 1592, 2251, 2824, 3424
AvaI CYCGRG 1 cut(s) 1632
AvaII GGWCC 4 cut(s) 98, 1835, 2843, 3583
AxyI CCTNAGG 2 cut(s) 1557, 1863
BaeGI GKGCMC 2 cut(s) 2074, 3679
BaeI ACNNNNGTAYC 4 cut(s) 1560, 1593, 3552, 3585
BamHI GGATCC 1 cut(s) 1858
BanI GGYRCC 2 cut(s) 149, 3640
BanII GRGCYC 2 cut(s) 2950, 3044
BarI GAAGNNNNNNTAC 2 cut(s) 2441, 2473
BauI CACGAG 2 cut(s) 729, 3624
Bbv12I GWGCWC 6 cut(s) 549, 1687, 1778, 2074, 3044, 3679
BccI CCATC 8 cut(s) 20, 633, 1478, 1773, 2809, 3350, 3467, 3851
BceAI ACGGC 2 cut(s) 1995, 3121
BcgI CGANNNNNNTGC 2 cut(s) 2421, 2455
BciT130I CCWGG 3 cut(s) 653, 1430, 3227
BciVI GTATCC 3 cut(s) 596, 2253, 3207
BclI TGATCA 5 cut(s) 7, 414, 1933, 2302, 2488
BcoDI GTCTC 7 cut(s) 421, 1100, 1170, 1963, 2161, 3388, 3830
BfoI RGCGCY 1 cut(s) 3644
BfuI GTATCC 3 cut(s) 596, 2253, 3207
BlpI GCTNAGC 1 cut(s) 1518
Bme1390I CCNGG 3 cut(s) 653, 1430, 3227
Bme18I GGWCC 4 cut(s) 98, 1835, 2843, 3583
BmeRI GACNNNNNGTC 2 cut(s) 3109, 3246
BmeT110I CYCGRG 1 cut(s) 1632
BmgBI CACGTC 1 cut(s) 2986
BmgT120I GGNCC 5 cut(s) 98, 1606, 1835, 2843, 3583
BmiI GGNNCC 8 cut(s) 151, 1030, 1434, 1607, 1860, 2947, 3584, 3642
BmrFI CCNGG 3 cut(s) 653, 1430, 3227
BmrI ACTGGG 1 cut(s) 2192
BmuI ACTGGG 1 cut(s) 2192
BoxI GACNNNNGTC 1 cut(s) 1751
BpmI CTGGAG 3 cut(s) 531, 2112, 2964
Bpu1102I GCTNAGC 1 cut(s) 1518
BpuEI CTTGAG 1 cut(s) 1792
BsaBI GATNNNNATC 2 cut(s) 30, 3848
BsaHI GRCGYC 1 cut(s) 3641
BsaJI CCNNGG 6 cut(s) 20, 195, 561, 651, 1237, 2011
BsaWI WCCGGW 3 cut(s) 100, 419, 1743
BsaXI ACNNNNNCTCC 6 cut(s) 558, 588, 981, 1011, 2110, 2140
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 1349, 1965, 2255, 2669, 3338, 3664
Bse118I RCCGGY 2 cut(s) 1349, 2600
Bse1I ACTGG 8 cut(s) 514, 589, 1030, 1728, 1927, 2198, 2271, 3672
Bse21I CCTNAGG 2 cut(s) 1557, 1863
Bse3DI GCAATG 3 cut(s) 1797, 3456, 3813
Bse8I GATNNNNATC 2 cut(s) 30, 3848
BseBI CCWGG 3 cut(s) 653, 1430, 3227
BseDI CCNNGG 6 cut(s) 20, 195, 561, 651, 1237, 2011
BseJI GATNNNNATC 2 cut(s) 30, 3848
BseLI CCNNNNNNNGG 7 cut(s) 26, 1349, 1965, 2255, 2669, 3338, 3664
BseMI GCAATG 3 cut(s) 1797, 3456, 3813
BseMII CTCAG 8 cut(s) 1178, 1509, 1571, 1692, 1854, 2300, 3047, 3678
BseNI ACTGG 8 cut(s) 514, 589, 1030, 1728, 1927, 2198, 2271, 3672
BseRI GAGGAG 2 cut(s) 1005, 1188
BseSI GKGCMC 2 cut(s) 2074, 3679
BsgI GTGCAG 3 cut(s) 1733, 2736, 3801
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 7 cut(s) 560, 1064, 1349, 1608, 1945, 2604, 3668
BshNI GGYRCC 2 cut(s) 149, 3640
BsiHKAI GWGCWC 6 cut(s) 549, 1687, 1778, 2074, 3044, 3679
BsiHKCI CYCGRG 1 cut(s) 1632
BsiSI CCGG 6 cut(s) 83, 101, 420, 1350, 1744, 2601
BslFI GGGAC 1 cut(s) 1575
BslI CCNNNNNNNGG 7 cut(s) 26, 1349, 1965, 2255, 2669, 3338, 3664
BsmAI GTCTC 7 cut(s) 421, 1100, 1170, 1963, 2161, 3388, 3830
BsmFI GGGAC 1 cut(s) 1575
BsmI GAATGC 2 cut(s) 1792, 3484
BsnI GGCC 7 cut(s) 560, 1064, 1349, 1608, 1945, 2604, 3668
BsoBI CYCGRG 1 cut(s) 1632
Bsp1286I GDGCHC 7 cut(s) 549, 1687, 1778, 2074, 2950, 3044, 3679
Bsp1720I GCTNAGC 1 cut(s) 1518
Bsp19I CCATGG 2 cut(s) 195, 561
BspACI CCGC 9 cut(s) 57, 675, 957, 1065, 1293, 2167, 2531, 2880, 3577
BspANI GGCC 7 cut(s) 560, 1064, 1349, 1608, 1945, 2604, 3668
BspCNI CTCAG 8 cut(s) 1179, 1510, 1570, 1693, 1855, 2301, 3046, 3679
BspFNI CGCG 1 cut(s) 59
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 8 cut(s) 151, 1030, 1434, 1607, 1860, 2947, 3584, 3642
BspMAI CTGCAG 6 cut(s) 382, 1090, 2227, 2596, 2933, 3508
BspPI GGATC 8 cut(s) 1409, 1853, 1866, 1995, 2023, 2188, 3237, 3741
BspQI GCTCTTC 2 cut(s) 182, 2020
BspT107I GGYRCC 2 cut(s) 149, 3640
BsrBI CCGCTC 3 cut(s) 677, 1293, 3577
BsrDI GCAATG 3 cut(s) 1797, 3456, 3813
BsrFI RCCGGY 2 cut(s) 1349, 2600
BsrI ACTGG 8 cut(s) 514, 589, 1030, 1728, 1927, 2198, 2271, 3672
BssAI RCCGGY 2 cut(s) 1349, 2600
BssECI CCNNGG 6 cut(s) 20, 195, 561, 651, 1237, 2011
BssNI GRCGYC 1 cut(s) 3641
BssSI CACGAG 2 cut(s) 729, 3624
BssT1I CCWWGG 3 cut(s) 20, 195, 561
Bst2BI CACGAG 2 cut(s) 729, 3624
Bst2UI CCWGG 3 cut(s) 653, 1430, 3227
Bst6I CTCTTC 5 cut(s) 182, 1494, 2020, 2419, 2799
BstACI GRCGYC 1 cut(s) 3641
BstAPI GCANNNNNTGC 4 cut(s) 1274, 2078, 3683, 3887
BstC8I GCNNGC 7 cut(s) 130, 1524, 1880, 2602, 2746, 2929, 2965
BstDSI CCRYGG 4 cut(s) 195, 561, 1237, 2011
BstENI CCTNNNNNAGG 3 cut(s) 2667, 3336, 3662
BstFNI CGCG 1 cut(s) 59
BstH2I RGCGCY 1 cut(s) 3644
BstHHI GCGC 2 cut(s) 233, 3643
BstMAI GTCTC 7 cut(s) 421, 1100, 1170, 1963, 2161, 3388, 3830
BstNI CCWGG 3 cut(s) 653, 1430, 3227
BstNSI RCATGY 1 cut(s) 3890
BstPAI GACNNNNGTC 1 cut(s) 1751
BstSCI CCNGG 3 cut(s) 651, 1428, 3225
BstSLI GKGCMC 2 cut(s) 2074, 3679
BstUI CGCG 1 cut(s) 59
BstX2I RGATCY 4 cut(s) 1858, 2000, 2193, 3229
BstXI CCANNNNNNTGG 1 cut(s) 322
BstYI RGATCY 4 cut(s) 1858, 2000, 2193, 3229
Bsu36I CCTNAGG 2 cut(s) 1557, 1863
BsuI GTATCC 3 cut(s) 596, 2253, 3207
BsuRI GGCC 7 cut(s) 560, 1064, 1349, 1608, 1945, 2604, 3668
BtgI CCRYGG 4 cut(s) 195, 561, 1237, 2011
BtgZI GCGATG 1 cut(s) 1751
BtrI CACGTC 1 cut(s) 2986
BtsI GCAGTG 2 cut(s) 84, 3801
Cac8I GCNNGC 7 cut(s) 130, 1524, 1880, 2602, 2746, 2929, 2965
CaiI CAGNNNCTG 2 cut(s) 761, 1700
CciI TCATGA 1 cut(s) 699
CfoI GCGC 2 cut(s) 233, 3643
Cfr10I RCCGGY 2 cut(s) 1349, 2600
Cfr13I GGNCC 5 cut(s) 98, 1606, 1835, 2843, 3583
CpoI CGGWCCG 1 cut(s) 98
CseI GACGC 4 cut(s) 48, 1700, 2750, 3719
Csp6I GTAC 6 cut(s) 161, 222, 835, 2214, 2734, 2824
CspCI CAANNNNNGTGG 6 cut(s) 127, 162, 3368, 3403, 3729, 3764
CspI CGGWCCG 1 cut(s) 98
CviQI GTAC 6 cut(s) 161, 222, 835, 2214, 2734, 2824
DinI GGCGCC 1 cut(s) 3642
DraI TTTAAA 1 cut(s) 351
DrdI GACNNNNNNGTC 1 cut(s) 1977
DriI GACNNNNNGTC 2 cut(s) 3109, 3246
DseDI GACNNNNNNGTC 1 cut(s) 1977
EaeI YGGCCR 1 cut(s) 1062
Eam1104I CTCTTC 5 cut(s) 182, 1494, 2020, 2419, 2799
Eam1105I GACNNNNNGTC 2 cut(s) 3109, 3246
EarI CTCTTC 5 cut(s) 182, 1494, 2020, 2419, 2799
EciI GGCGGA 1 cut(s) 972
Ecl136II GAGCTC 1 cut(s) 3042
Eco130I CCWWGG 3 cut(s) 20, 195, 561
Eco24I GRGCYC 2 cut(s) 2950, 3044
Eco47I GGWCC 4 cut(s) 98, 1835, 2843, 3583
Eco53kI GAGCTC 1 cut(s) 3042
Eco57I CTGAAG 2 cut(s) 1518, 2823
Eco81I CCTNAGG 2 cut(s) 1557, 1863
Eco88I CYCGRG 1 cut(s) 1632
EcoICRI GAGCTC 1 cut(s) 3042
EcoNI CCTNNNNNAGG 3 cut(s) 2667, 3336, 3662
EcoO109I RGGNCCY 1 cut(s) 2843
EcoRI GAATTC 2 cut(s) 310, 1656
EcoRII CCWGG 3 cut(s) 651, 1428, 3225
EcoT14I CCWWGG 3 cut(s) 20, 195, 561
EcoT38I GRGCYC 2 cut(s) 2950, 3044
EgeI GGCGCC 1 cut(s) 3642
EheI GGCGCC 1 cut(s) 3642
ErhI CCWWGG 3 cut(s) 20, 195, 561
FalI AAGNNNNNCTT 6 cut(s) 1975, 2007, 2145, 2177, 2273, 2305
FaqI GGGAC 1 cut(s) 1575
FauI CCCGC 2 cut(s) 682, 3570
FauNDI CATATG 2 cut(s) 2062, 3463
FbaI TGATCA 5 cut(s) 7, 414, 1933, 2302, 2488
FblI GTMKAC 2 cut(s) 360, 3296
FriOI GRGCYC 2 cut(s) 2950, 3044
GlaI GCGC 2 cut(s) 232, 3642
GsuI CTGGAG 3 cut(s) 531, 2112, 2964
HaeII RGCGCY 1 cut(s) 3644
HaeIII GGCC 7 cut(s) 560, 1064, 1349, 1608, 1945, 2604, 3668
HapII CCGG 6 cut(s) 83, 101, 420, 1350, 1744, 2601
HgaI GACGC 4 cut(s) 48, 1700, 2750, 3719
HhaI GCGC 2 cut(s) 233, 3643
Hin1I GRCGYC 1 cut(s) 3641
Hin6I GCGC 2 cut(s) 231, 3641
HinP1I GCGC 2 cut(s) 231, 3641
HincII GTYRAC 3 cut(s) 1023, 1741, 3297
HindII GTYRAC 3 cut(s) 1023, 1741, 3297
HindIII AAGCTT 1 cut(s) 855
HpaI GTTAAC 1 cut(s) 1741
HpaII CCGG 6 cut(s) 83, 101, 420, 1350, 1744, 2601
HphI GGTGA 8 cut(s) 17, 409, 548, 1364, 1592, 2251, 2824, 3424
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 9 cut(s) 565, 1042, 1474, 2296, 2443, 2803, 2834, 3154, 3631
HpyCH4IV ACGT 3 cut(s) 480, 2985, 3213
HpySE526I ACGT 3 cut(s) 480, 2985, 3213
Hsp92I GRCGYC 1 cut(s) 3641
HspAI GCGC 2 cut(s) 231, 3641
KasI GGCGCC 1 cut(s) 3640
KroI GCCGGC 1 cut(s) 2600
KroNI GCCGGC 1 cut(s) 2602
Ksp22I TGATCA 5 cut(s) 7, 414, 1933, 2302, 2488
KspAI GTTAAC 1 cut(s) 1741
LguI GCTCTTC 2 cut(s) 182, 2020
LmnI GCTCC 6 cut(s) 374, 1034, 1868, 2131, 2945, 3047
MaeII ACGT 3 cut(s) 480, 2985, 3213
MaeIII GTNAC 5 cut(s) 113, 910, 3128, 3341, 3554
MbiI CCGCTC 3 cut(s) 677, 1293, 3577
MfeI CAATTG 8 cut(s) 234, 777, 1821, 2505, 2633, 2784, 3380, 3618
MflI RGATCY 4 cut(s) 1858, 2000, 2193, 3229
MhlI GDGCHC 7 cut(s) 549, 1687, 1778, 2074, 2950, 3044, 3679
Mly113I GGCGCC 1 cut(s) 3641
MlyI GAGTC 5 cut(s) 2492, 3116, 3128, 3698, 3708
MmeI TCCRAC 4 cut(s) 2533, 3004, 3224, 3253
MroNI GCCGGC 1 cut(s) 2600
MroXI GAANNNNTTC 2 cut(s) 2708, 3073
MslI CAYNNNNRTG 3 cut(s) 1054, 2496, 3891
MspA1I CMGCKG 3 cut(s) 383, 1393, 3020
MspI CCGG 6 cut(s) 83, 101, 420, 1350, 1744, 2601
MspR9I CCNGG 3 cut(s) 653, 1430, 3227
MunI CAATTG 8 cut(s) 234, 777, 1821, 2505, 2633, 2784, 3380, 3618
Mva1269I GAATGC 2 cut(s) 1792, 3484
MvaI CCWGG 3 cut(s) 653, 1430, 3227
MvnI CGCG 1 cut(s) 59
NaeI GCCGGC 1 cut(s) 2602
NarI GGCGCC 1 cut(s) 3641
NcoI CCATGG 2 cut(s) 195, 561
NdeI CATATG 2 cut(s) 2062, 3463
NgoMIV GCCGGC 1 cut(s) 2600
NlaIV GGNNCC 8 cut(s) 151, 1030, 1434, 1607, 1860, 2947, 3584, 3642
NmuCI GTSAC 2 cut(s) 3128, 3341
NspI RCATGY 1 cut(s) 3890
PaeR7I CTCGAG 1 cut(s) 1632
PagI TCATGA 1 cut(s) 699
PciSI GCTCTTC 2 cut(s) 182, 2020
PctI GAATGC 2 cut(s) 1792, 3484
PdiI GCCGGC 1 cut(s) 2602
PdmI GAANNNNTTC 2 cut(s) 2708, 3073
PflFI GACNNNGTC 1 cut(s) 1370
PfoI TCCNGGA 1 cut(s) 3225
PleI GAGTC 5 cut(s) 2491, 3116, 3128, 3697, 3707
PluTI GGCGCC 1 cut(s) 3644
PpsI GAGTC 5 cut(s) 2491, 3116, 3128, 3697, 3707
PpuMI RGGWCCY 1 cut(s) 2843
PshAI GACNNNNGTC 1 cut(s) 1751
PshBI ATTAAT 3 cut(s) 288, 2336, 3846
PsiI TTATAA 3 cut(s) 1095, 1886, 2645
Psp124BI GAGCTC 1 cut(s) 3044
Psp5II RGGWCCY 1 cut(s) 2843
Psp6I CCWGG 3 cut(s) 651, 1428, 3225
PspGI CCWGG 3 cut(s) 651, 1428, 3225
PspN4I GGNNCC 8 cut(s) 151, 1030, 1434, 1607, 1860, 2947, 3584, 3642
PspPI GGNCC 5 cut(s) 98, 1606, 1835, 2843, 3583
PspPPI RGGWCCY 1 cut(s) 2843
PspXI VCTCGAGB 1 cut(s) 1632
PstI CTGCAG 6 cut(s) 382, 1090, 2227, 2596, 2933, 3508
PstNI CAGNNNCTG 2 cut(s) 761, 1700
PsuI RGATCY 4 cut(s) 1858, 2000, 2193, 3229
PsyI GACNNNGTC 1 cut(s) 1370
PvuII CAGCTG 3 cut(s) 383, 1393, 3020
RsaI GTAC 6 cut(s) 162, 223, 836, 2215, 2735, 2825
RsaNI GTAC 6 cut(s) 161, 222, 835, 2214, 2734, 2824
RseI CAYNNNNRTG 3 cut(s) 1054, 2496, 3891
Rsr2I CGGWCCG 1 cut(s) 98
RsrII CGGWCCG 1 cut(s) 98
SacI GAGCTC 1 cut(s) 3044
SalI GTCGAC 1 cut(s) 3295
SapI GCTCTTC 2 cut(s) 182, 2020
Sau96I GGNCC 5 cut(s) 98, 1606, 1835, 2843, 3583
SchI GAGTC 5 cut(s) 2492, 3116, 3128, 3698, 3708
ScrFI CCNGG 3 cut(s) 653, 1430, 3227
SduI GDGCHC 7 cut(s) 549, 1687, 1778, 2074, 2950, 3044, 3679
SfoI GGCGCC 1 cut(s) 3642
Sfr274I CTCGAG 1 cut(s) 1632
SinI GGWCC 4 cut(s) 98, 1835, 2843, 3583
SlaI CTCGAG 1 cut(s) 1632
SmiMI CAYNNNNRTG 3 cut(s) 1054, 2496, 3891
SmlI CTYRAG 2 cut(s) 1632, 1771
SmoI CTYRAG 2 cut(s) 1632, 1771
SsiI CCGC 9 cut(s) 57, 675, 957, 1065, 1293, 2167, 2531, 2880, 3577
SspDI GGCGCC 1 cut(s) 3640
SspI AATATT 2 cut(s) 468, 1468
SstI GAGCTC 1 cut(s) 3044
StyD4I CCNGG 3 cut(s) 651, 1428, 3225
StyI CCWWGG 3 cut(s) 20, 195, 561
TaiI ACGT 3 cut(s) 483, 2988, 3216
TatI WGTACW 1 cut(s) 834
TauI GCSGC 4 cut(s) 59, 1067, 2169, 2534
TseFI GTSAC 2 cut(s) 3128, 3341
Tsp45I GTSAC 2 cut(s) 3128, 3341
TspGWI ACGGA 2 cut(s) 111, 1226
Tth111I GACNNNGTC 1 cut(s) 1370
VneI GTGCAC 2 cut(s) 2070, 3675
VpaK11BI GGWCC 4 cut(s) 98, 1835, 2843, 3583
VspI ATTAAT 3 cut(s) 288, 2336, 3846
XagI CCTNNNNNAGG 3 cut(s) 2667, 3336, 3662
XapI RAATTY 5 cut(s) 310, 1538, 1656, 2581, 2975
XbaI TCTAGA 1 cut(s) 1666
XceI RCATGY 1 cut(s) 3890
XcmI CCANNNNNNNNNTGG 2 cut(s) 2764, 3593
XhoI CTCGAG 1 cut(s) 1632
XmiI GTMKAC 2 cut(s) 360, 3296
XmnI GAANNNNTTC 2 cut(s) 2708, 3073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.