MD16G1058500.v1.1

Floral homeotic protein APETALA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002429
Physical Location & Seq
Reverse (-)
4172876 .. 4176644
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1058500.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGGGAGAGGTAGAGTTCAGCTTAAGCGCATAGAGAACAAGATCAACAGGCAGGTGACTTTTTCCAAAAGAAGAACTGGGCTGCTGAAGAAGGCTCATGAGATCTCTGTCTTGTGTGATGCTCAGGTTGCTTTGATTGTCTTCTCCAACAAGGGCAAGCTCTTTGAGTACGCCACAGATTCATGCATGGAGCAAATCCTCGAACGCTATGAAAGATACTCTTACGCCGAAAGACAGCTAGTCGAACCAGATTTTGAGTCACAGGGTAACTGGACCTTTGAATATTCCAGACTAAAGGCTAAGGCTGAGGTTTTGCAAAGAAACCATAGGCACTATTTGGGAGAAGACCTGGATTCCTTGACTCTCAAGGAAATCCAAAATTTGGAGCAACAGCTTGACACCGCTCTTAAACAAATTCGATTAAGAAAAAACCAACTCATGAATGAATCCATCTCTGAGCTTCAGAGAAAGAGAAAGGCGATACAGGAGGAAAATAACTTGCTAGCAAAGAAGATAAAGGAGAAGGAGAAGGCTGCAGCTCAGCCCCAGGTTCAAAACTGGGAGCAGCAGAACCATGGCCTTGACCTGCTTCCACAGCCACTTCCATGTCTAAACAATGGGGGGACGCAGCAAGATGAATTCCTTCAAGTGAGGAGGAACCAGCTGGACCTGACTCTGGAACCATTATATGAATGCCATCTCGGATGCTTTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

240

Amino Acids

27.98

Weight (kDa)

8.72

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 8e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 87 - 172 5e-29 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 2 cut(s) 43, 594
AccB7I CCANNNNNTGG 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 404
AciI CCGC 1 cut(s) 402
AcsI RAATTY 3 cut(s) 379, 414, 638
AcuI CTGAAG 2 cut(s) 107, 446
AfaI GTAC 1 cut(s) 170
AfiI CCNNNNNNNGG 2 cut(s) 382, 676
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 3 cut(s) 281, 554, 647
AhdI GACNNNNNGTC 1 cut(s) 239
AjnI CCWGG 2 cut(s) 348, 546
AluBI AGCT 7 cut(s) 22, 160, 238, 394, 460, 539, 664
AluI AGCT 7 cut(s) 22, 160, 238, 394, 460, 539, 664
AoxI GGCC 1 cut(s) 577
ApeKI GCWGC 6 cut(s) 82, 533, 536, 565, 628, 713
ApoI RAATTY 3 cut(s) 379, 414, 638
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 273, 667
AsuHPI GGTGA 1 cut(s) 67
AsuNHI GCTAGC 1 cut(s) 502
AvaII GGWCC 2 cut(s) 273, 667
BbsI GAAGAC 2 cut(s) 133, 351
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 6 cut(s) 69, 520, 548, 577, 640, 700
BccI CCATC 2 cut(s) 458, 705
BciT130I CCWGG 2 cut(s) 350, 548
BfaI CTAG 2 cut(s) 239, 503
BfmI CTRYAG 1 cut(s) 534
BfrI CTTAAG 1 cut(s) 23
BfuAI ACCTGC 2 cut(s) 43, 594
BglII AGATCT 1 cut(s) 102
BisI GCNGC 6 cut(s) 83, 534, 537, 566, 629, 714
BlpI GCTNAGC 1 cut(s) 540
BlsI GCNGC 6 cut(s) 84, 535, 538, 567, 630, 715
Bme1390I CCNGG 2 cut(s) 350, 548
Bme18I GGWCC 2 cut(s) 273, 667
BmeRI GACNNNNNGTC 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 273, 667
BmiI GGNNCC 2 cut(s) 659, 681
BmrFI CCNGG 2 cut(s) 350, 548
BmrI ACTGGG 2 cut(s) 87, 568
BmsI GCATC 2 cut(s) 109, 695
BmtI GCTAGC 1 cut(s) 506
BmuI ACTGGG 2 cut(s) 87, 568
BpiI GAAGAC 2 cut(s) 133, 351
Bpu10I CCTNAGC 3 cut(s) 123, 300, 306
Bpu1102I GCTNAGC 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 546, 574
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 676
Bse1I ACTGG 3 cut(s) 82, 275, 563
BseBI CCWGG 2 cut(s) 350, 548
BseDI CCNNGG 2 cut(s) 546, 574
BseGI GGATG 1 cut(s) 710
BseLI CCNNNNNNNGG 2 cut(s) 382, 676
BseMII CTCAG 4 cut(s) 137, 297, 447, 554
BseNI ACTGG 3 cut(s) 82, 275, 563
BseRI GAGGAG 1 cut(s) 667
BseXI GCAGC 6 cut(s) 69, 520, 548, 577, 640, 700
BshFI GGCC 1 cut(s) 579
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 2 cut(s) 382, 676
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 1 cut(s) 579
Bsp143I GATC 2 cut(s) 42, 102
Bsp1720I GCTNAGC 1 cut(s) 540
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 1 cut(s) 402
BspANI GGCC 1 cut(s) 579
BspCNI CTCAG 4 cut(s) 136, 298, 448, 553
BspHI TCATGA 2 cut(s) 97, 438
BspLI GGNNCC 2 cut(s) 659, 681
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 2 cut(s) 43, 594
BspOI GCTAGC 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 23
BsrBI CCGCTC 1 cut(s) 404
BsrI ACTGG 3 cut(s) 82, 275, 563
BssECI CCNNGG 2 cut(s) 546, 574
BssMI GATC 2 cut(s) 42, 102
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 350, 548
BstAFI CTTAAG 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 158, 504
BstDEI CTNAG 5 cut(s) 123, 300, 306, 456, 540
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 1 cut(s) 710
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 2 cut(s) 45, 105
BstMBI GATC 2 cut(s) 42, 102
BstMWI GCNNNNNNNGC 2 cut(s) 128, 595
BstNI CCWGG 2 cut(s) 350, 548
BstSCI CCNGG 2 cut(s) 348, 546
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 6 cut(s) 69, 520, 548, 577, 640, 700
BstV2I GAAGAC 2 cut(s) 133, 351
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 1 cut(s) 579
BtgI CCRYGG 1 cut(s) 574
BtsCI GGATG 1 cut(s) 710
BveI ACCTGC 2 cut(s) 43, 594
Cac8I GCNNGC 2 cut(s) 158, 504
CciI TCATGA 2 cut(s) 97, 438
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 2 cut(s) 273, 667
CseI GACGC 1 cut(s) 634
Csp6I GTAC 1 cut(s) 169
CviAII CATG 6 cut(s) 98, 183, 187, 439, 575, 606
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 5 cut(s) 123, 300, 306, 456, 540
DpnI GATC 2 cut(s) 44, 104
DpnII GATC 2 cut(s) 42, 102
DriI GACNNNNNGTC 1 cut(s) 239
Eam1105I GACNNNNNGTC 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 574
Eco47I GGWCC 2 cut(s) 273, 667
Eco57I CTGAAG 2 cut(s) 107, 446
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 348, 546
EcoT14I CCWWGG 1 cut(s) 574
EcoT22I ATGCAT 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 6 cut(s) 101, 186, 190, 442, 578, 609
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FaqI GGGAC 1 cut(s) 637
FatI CATG 6 cut(s) 97, 182, 186, 438, 574, 605
Fnu4HI GCNGC 6 cut(s) 83, 534, 537, 566, 629, 714
Fsp4HI GCNGC 6 cut(s) 83, 534, 537, 566, 629, 714
FspBI CTAG 2 cut(s) 239, 503
GlaI GCGC 1 cut(s) 29
GluI GCNGC 6 cut(s) 83, 534, 537, 566, 629, 714
HaeIII GGCC 1 cut(s) 579
HgaI GACGC 1 cut(s) 634
HhaI GCGC 1 cut(s) 30
Hin1II CATG 6 cut(s) 101, 186, 190, 442, 578, 609
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HinfI GANTC 6 cut(s) 179, 257, 353, 361, 446, 673
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 457, 465, 704
Hpy188III TCNNGA 4 cut(s) 98, 288, 439, 677
HpyAV CCTTC 4 cut(s) 85, 517, 523, 653
HpyCH4V TGCA 3 cut(s) 186, 316, 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 595
HpyF3I CTNAG 5 cut(s) 123, 300, 306, 456, 540
Hsp92II CATG 6 cut(s) 101, 186, 190, 442, 578, 609
HspAI GCGC 1 cut(s) 28
Kzo9I GATC 2 cut(s) 42, 102
LmnI GCTCC 3 cut(s) 190, 385, 562
Lsp1109I GCAGC 6 cut(s) 69, 520, 548, 577, 640, 700
LweI GCATC 2 cut(s) 109, 695
MaeI CTAG 2 cut(s) 239, 503
MaeIII GTNAC 3 cut(s) 55, 258, 266
MalI GATC 2 cut(s) 44, 104
MbiI CCGCTC 1 cut(s) 404
MboI GATC 2 cut(s) 42, 102
MboII GAAGA 5 cut(s) 84, 100, 133, 356, 523
MflI RGATCY 1 cut(s) 102
MluCI AATT 3 cut(s) 379, 414, 638
MlyI GAGTC 3 cut(s) 266, 355, 667
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 5 cut(s) 209, 301, 481, 645, 648
Mph1103I ATGCAT 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 3 cut(s) 24, 408, 422
MslI CAYNNNNRTG 1 cut(s) 604
MspA1I CMGCKG 1 cut(s) 664
MspCI CTTAAG 1 cut(s) 23
MspR9I CCNGG 2 cut(s) 350, 548
Mva1269I GAATGC 1 cut(s) 698
MvaI CCWGG 2 cut(s) 350, 548
MwoI GCNNNNNNNGC 2 cut(s) 128, 595
NcoI CCATGG 1 cut(s) 574
NdeII GATC 2 cut(s) 42, 102
NheI GCTAGC 1 cut(s) 502
NlaIII CATG 6 cut(s) 101, 186, 190, 442, 578, 609
NlaIV GGNNCC 2 cut(s) 659, 681
NmuCI GTSAC 2 cut(s) 55, 258
NsiI ATGCAT 1 cut(s) 188
PagI TCATGA 2 cut(s) 97, 438
PaqCI CACCTGC 1 cut(s) 43
PctI GAATGC 1 cut(s) 698
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 3 cut(s) 179, 353, 446
PflMI CCANNNNNTGG 1 cut(s) 382
PkrI GCNGC 6 cut(s) 84, 535, 538, 567, 630, 715
PleI GAGTC 3 cut(s) 265, 355, 667
PpsI GAGTC 3 cut(s) 265, 355, 667
Psp6I CCWGG 2 cut(s) 348, 546
PspGI CCWGG 2 cut(s) 348, 546
PspN4I GGNNCC 2 cut(s) 659, 681
PspPI GGNCC 2 cut(s) 273, 667
PstI CTGCAG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 664
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 1 cut(s) 604
SaqAI TTAA 3 cut(s) 24, 408, 422
SatI GCNGC 6 cut(s) 83, 534, 537, 566, 629, 714
Sau3AI GATC 2 cut(s) 42, 102
Sau96I GGNCC 2 cut(s) 273, 667
SchI GAGTC 3 cut(s) 266, 355, 667
ScrFI CCNGG 2 cut(s) 350, 548
SfaNI GCATC 2 cut(s) 109, 695
SfcI CTRYAG 1 cut(s) 534
SinI GGWCC 2 cut(s) 273, 667
SmiMI CAYNNNNRTG 1 cut(s) 604
SmlI CTYRAG 2 cut(s) 23, 365
SmoI CTYRAG 2 cut(s) 23, 365
Sse9I AATT 3 cut(s) 379, 414, 638
SsiI CCGC 1 cut(s) 402
SspI AATATT 1 cut(s) 284
SspMI CTAG 2 cut(s) 239, 503
StyD4I CCNGG 2 cut(s) 348, 546
StyI CCWWGG 1 cut(s) 574
TaqI TCGA 3 cut(s) 201, 243, 418
TasI AATT 3 cut(s) 379, 414, 638
TfiI GAWTC 3 cut(s) 179, 353, 446
Tru1I TTAA 3 cut(s) 24, 408, 422
Tru9I TTAA 3 cut(s) 24, 408, 422
TseFI GTSAC 2 cut(s) 55, 258
TseI GCWGC 6 cut(s) 82, 533, 536, 565, 628, 713
Tsp45I GTSAC 2 cut(s) 55, 258
TspDTI ATGAA 6 cut(s) 171, 225, 455, 459, 651, 705
Van91I CCANNNNNTGG 1 cut(s) 382
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 2 cut(s) 273, 667
XapI RAATTY 3 cut(s) 379, 414, 638
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 2 cut(s) 239, 503
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.