Rh4DG361700

Floral homeotic protein APETALA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002429
Physical Location & Seq
Reverse (-)
58355245 .. 58358695
3451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG361700.1

Sequence Viewer

Length: 558 bp
ATGGAGAATATACTTGAACGCTATGAGCGGTACTCGTACGCAGAGAGACAGCTAGTCGAACCTGATTTGGAATCACAGGGTAACTGGACCTTCGAACATGCTAGACTGAAGGTGAAAGTTGAGCTTCTGCAAAGAAACCTTAGGCACTATTTGGGAGAAGATCTGGATTCATTGAGTATCAAAGAGATCCAAAGTTTGGAGCAACAGCTTGACAATTCTCTTAAGCAAATTCGATCAAGAAAGAACCAACTCATGCATGAGTCTATGTCCGAACTTCAGAGAAAGGAGAAGGCAATGCAGGAGCAAAATAACTTGCTTTCAAAGAAGATTAAGGAGAAGGAGAAGAATGTAGCGGAAGCTCAGGAGGTTCATAATTGGGAGCAGCAGCAACAGCAGCAAAACCATGGCCTTAACCTGGTAGCACAGGCTCCACTTCCATGTTTGAACATGGGGGGCACTCAGCAAAATGATCAGTTCCTTCAAGTGAGGAGGAACCAGCTCGACCTTACTCTCGAGCCATCGTTGTATTCATGCCACCTTGGATGCTTTGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

185

Amino Acids

21.82

Weight (kDa)

5.9

Isoelectric Point (pI)

62.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 25 - 111 1e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 28
AciI CCGC 2 cut(s) 28, 353
AclWI GGATC 1 cut(s) 181
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 2 cut(s) 128, 260
AfaI GTAC 2 cut(s) 32, 38
AfiI CCNNNNNNNGG 2 cut(s) 196, 415
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 4 cut(s) 17, 321, 445, 482
AhdI GACNNNNNGTC 1 cut(s) 53
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 5 cut(s) 52, 124, 208, 359, 499
AluI AGCT 5 cut(s) 52, 124, 208, 359, 499
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 181
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 3 cut(s) 382, 385, 394
ApoI RAATTY 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 93
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 458
BbvI GCAGC 3 cut(s) 394, 397, 406
BccI CCATC 1 cut(s) 526
BciT130I CCWGG 1 cut(s) 416
BclI TGATCA 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 2 cut(s) 53, 102
BfrI CTTAAG 1 cut(s) 221
BglII AGATCT 1 cut(s) 160
BisI GCNGC 3 cut(s) 383, 386, 395
BlsI GCNGC 3 cut(s) 384, 387, 396
Bme1390I CCNGG 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 87
BmeRI GACNNNNNGTC 1 cut(s) 53
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 429, 494
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 1 cut(s) 533
BplI GAGNNNNNCTC 2 cut(s) 17, 49
Bpu10I CCTNAGC 1 cut(s) 360
Bpu14I TTCGAA 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 403, 538
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 415
Bse1I ACTGG 1 cut(s) 89
Bse21I CCTNAGG 1 cut(s) 140
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 2 cut(s) 403, 538
BseGI GGATG 1 cut(s) 548
BseLI CCNNNNNNNGG 2 cut(s) 196, 415
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 2 cut(s) 374, 473
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 502
BseSI GKGCMC 1 cut(s) 458
BseXI GCAGC 3 cut(s) 394, 397, 406
BshFI GGCC 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 512
BsiWI CGTACG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 196, 415
BsmAI GTCTC 1 cut(s) 40
BsnI GGCC 1 cut(s) 408
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 458
Bsp143I GATC 4 cut(s) 160, 186, 233, 469
Bsp19I CCATGG 1 cut(s) 403
BspACI CCGC 2 cut(s) 28, 353
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 2 cut(s) 373, 472
BspHI TCATGA 1 cut(s) 554
BspLI GGNNCC 2 cut(s) 429, 494
BspPI GGATC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 93
BspTI CTTAAG 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 300
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 403, 538
BssMI GATC 4 cut(s) 160, 186, 233, 469
BssT1I CCWWGG 2 cut(s) 403, 538
Bst2UI CCWGG 1 cut(s) 416
BstAFI CTTAAG 1 cut(s) 221
BstBI TTCGAA 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 140, 360, 459
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 1 cut(s) 548
BstKTI GATC 4 cut(s) 163, 189, 236, 472
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 4 cut(s) 160, 186, 233, 469
BstMWI GCNNNNNNNGC 2 cut(s) 391, 394
BstNI CCWGG 1 cut(s) 416
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 458
BstV1I GCAGC 3 cut(s) 394, 397, 406
BstX2I RGATCY 2 cut(s) 160, 186
BstYI RGATCY 2 cut(s) 160, 186
Bsu36I CCTNAGG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 403
BtsCI GGATG 1 cut(s) 548
CciI TCATGA 1 cut(s) 554
Cfr13I GGNCC 1 cut(s) 87
CsiI ACCWGGT 1 cut(s) 414
Csp6I GTAC 2 cut(s) 31, 37
CviAII CATG 8 cut(s) 98, 253, 257, 404, 438, 448, 531, 555
CviJI RGCY 8 cut(s) 52, 124, 208, 359, 408, 428, 499, 517
CviKI_1 RGCY 8 cut(s) 52, 124, 208, 359, 408, 428, 499, 517
CviQI GTAC 2 cut(s) 31, 37
DdeI CTNAG 3 cut(s) 140, 360, 459
DpnI GATC 4 cut(s) 162, 188, 235, 471
DpnII GATC 4 cut(s) 160, 186, 233, 469
DriI GACNNNNNGTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 403, 538
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 128, 260
Eco81I CCTNAGG 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 414
EcoT14I CCWWGG 2 cut(s) 403, 538
EcoT22I ATGCAT 1 cut(s) 258
ErhI CCWWGG 2 cut(s) 403, 538
FaeI CATG 8 cut(s) 101, 256, 260, 407, 441, 451, 534, 558
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FatI CATG 8 cut(s) 97, 252, 256, 403, 437, 447, 530, 554
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 3 cut(s) 383, 386, 395
Fsp4HI GCNGC 3 cut(s) 383, 386, 395
FspBI CTAG 2 cut(s) 53, 102
GluI GCNGC 3 cut(s) 383, 386, 395
HaeIII GGCC 1 cut(s) 408
Hin1II CATG 8 cut(s) 101, 256, 260, 407, 441, 451, 534, 558
HinfI GANTC 3 cut(s) 71, 167, 260
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 271, 279
Hpy188III TCNNGA 5 cut(s) 164, 237, 362, 512, 555
HpyAV CCTTC 5 cut(s) 100, 103, 283, 331, 488
HpyCH4V TGCA 3 cut(s) 130, 256, 298
HpyF10VI GCNNNNNNNGC 2 cut(s) 391, 394
HpyF3I CTNAG 3 cut(s) 140, 360, 459
Hsp92II CATG 8 cut(s) 101, 256, 260, 407, 441, 451, 534, 558
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 4 cut(s) 160, 186, 233, 469
LmnI GCTCC 4 cut(s) 199, 301, 379, 433
Lsp1109I GCAGC 3 cut(s) 394, 397, 406
LweI GCATC 1 cut(s) 533
MabI ACCWGGT 1 cut(s) 414
MaeI CTAG 2 cut(s) 53, 102
MaeIII GTNAC 1 cut(s) 80
MalI GATC 4 cut(s) 162, 188, 235, 471
MbiI CCGCTC 1 cut(s) 28
MboI GATC 4 cut(s) 160, 186, 233, 469
MboII GAAGA 3 cut(s) 170, 337, 355
MflI RGATCY 2 cut(s) 160, 186
MhlI GDGCHC 1 cut(s) 458
MluCI AATT 3 cut(s) 214, 228, 373
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 3 cut(s) 358, 480, 483
Mph1103I ATGCAT 1 cut(s) 258
MseI TTAA 3 cut(s) 222, 330, 411
MslI CAYNNNNRTG 1 cut(s) 436
MspCI CTTAAG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 2 cut(s) 391, 394
NcoI CCATGG 1 cut(s) 403
NdeII GATC 4 cut(s) 160, 186, 233, 469
NlaIII CATG 8 cut(s) 101, 256, 260, 407, 441, 451, 534, 558
NlaIV GGNNCC 2 cut(s) 429, 494
NsiI ATGCAT 1 cut(s) 258
NspI RCATGY 1 cut(s) 101
NspV TTCGAA 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 512
PagI TCATGA 1 cut(s) 554
PfeI GAWTC 2 cut(s) 71, 167
Pfl23II CGTACG 1 cut(s) 36
PflMI CCANNNNNTGG 1 cut(s) 196
PkrI GCNGC 3 cut(s) 384, 387, 396
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspLI CGTACG 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 429, 494
PspPI GGNCC 1 cut(s) 87
PsuI RGATCY 2 cut(s) 160, 186
RsaI GTAC 2 cut(s) 32, 38
RsaNI GTAC 2 cut(s) 31, 37
RseI CAYNNNNRTG 1 cut(s) 436
SaqAI TTAA 3 cut(s) 222, 330, 411
SatI GCNGC 3 cut(s) 383, 386, 395
Sau3AI GATC 4 cut(s) 160, 186, 233, 469
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 458
SexAI ACCWGGT 1 cut(s) 414
SfaNI GCATC 1 cut(s) 533
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 1 cut(s) 87
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 436
SmlI CTYRAG 2 cut(s) 221, 512
SmoI CTYRAG 2 cut(s) 221, 512
Sse9I AATT 3 cut(s) 214, 228, 373
SsiI CCGC 2 cut(s) 28, 353
SspMI CTAG 2 cut(s) 53, 102
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 2 cut(s) 403, 538
TaqI TCGA 5 cut(s) 57, 93, 232, 501, 513
TasI AATT 3 cut(s) 214, 228, 373
TfiI GAWTC 2 cut(s) 71, 167
Tru1I TTAA 3 cut(s) 222, 330, 411
Tru9I TTAA 3 cut(s) 222, 330, 411
TseI GCWGC 3 cut(s) 382, 385, 394
TspDTI ATGAA 4 cut(s) 159, 359, 519, 543
Van91I CCANNNNNTGG 1 cut(s) 196
Vha464I CTTAAG 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 1 cut(s) 228
XceI RCATGY 1 cut(s) 101
XhoI CTCGAG 1 cut(s) 512
XspI CTAG 2 cut(s) 53, 102
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.