MD09G1224200.v1.1

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002169
Physical Location & Seq
Reverse (-)
27188298 .. 27194408
6111 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1224200.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGCCAGACGATAGCTCATCAGTTTCCGACCACGGCCAAACTATTTGTGTCACCGGAGCTGGAGGCTTCATTGCTTCATGGATGGTGAAGCTATTGCTAGAAAGAGGCTACACTGTTAAAGGAACCTTGAGAAACCCGGATGACCCCAAAAATGCTCATTTGAGGGAGCTTGAAGGAGCTACAGAGAGACTAAGTTTGTGCAAAGCTGATCTCCTTGATTATGAGAGCCTCAAAGAAGCCATTAACGGCTGTGATGGAGTTTTTCACACAGCATCACCTGTCACGGATGATCCAGAACAAATGGTGGAGCCGGCGGTGAACGGAACCAAGAATGTGATTCAAGCAGCGGCGGAAGCCAAGGTGAAACGGGTGGTCTTCACATCGTCAATTGGTGCGGTGTACATGGACCCCACCAGGGGTCCTGAAGTGGTGGTCGACGAGTCTTGTTGGAGTGATCTTGAATTTTGCAAGAACACCAAGAACTGGTACTGCTATGGGAAAGCAGTGGCAGAGCAGGCAGCATGGGAGGAGGCCAGAGAGAAAGGGGTGGACTTGGCGGTGGTGAACCCAGTGCTGGTGCTCGGACCACTTCTGCAACCAACTGTCAATGCCAGCATCATTCACATCCTCAAGTACCTGACTGGCTCGACCAAGACCTATGCCAATTCAGTTCAGGCCTATGTGCATGTTAGGGACGTCGCACTGGCTCACATTTTGGTCTATGAGACACCCTCTGCAGGAGGCAGATACCTTTGTGCTGAGAGTGTGCTCCACCGTGGCGACGTCGTGGAAATCCTGGCCAAATTCTTCCCGGAATACCCTATTCCCAACAAGTGTAAAGATAATGGGAAACCCAGAGCAGAACCATACAAGTTCACAAACCAGAAGCTACGAGACTTAGGGTTGGAGTTCACGCCAGTCAAACAAGCCCTATATGAAACTGTCAAGAGCTTGCAGGAGAAGGGTCACCTTCCAGTCCATACAAAACAAGAACAAGATTCCATTAAAATTCAATCTTAA

Protein Analysis

340

Amino Acids

37.32

Weight (kDa)

5.77

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 13 - 132 2.7e-14 NmrA-like family
KR PF08659 14 - 137 2.1e-06 KR domain
Epimerase PF01370 15 - 248 1.9e-29 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 15 - 134 2.5e-08 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 17 - 240 1.9e-22 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 17 - 263 6.4e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 17 - 199 2.8e-12 Male sterility protein
NAD_binding_10 PF13460 19 - 135 1.3e-18 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 699, 786
AccB7I CCANNNNNTGG 1 cut(s) 483
AccI GTMKAC 1 cut(s) 435
AciI CCGC 5 cut(s) 314, 347, 350, 395, 557
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 2 cut(s) 34, 798
AcsI RAATTY 3 cut(s) 461, 803, 1008
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 2 cut(s) 696, 783
AfaI GTAC 3 cut(s) 401, 488, 635
AfiI CCNNNNNNNGG 5 cut(s) 415, 416, 483, 574, 737
AgsI TTSAA 4 cut(s) 173, 341, 461, 1013
AjnI CCWGG 2 cut(s) 413, 795
AluBI AGCT 8 cut(s) 15, 59, 91, 169, 179, 206, 889, 951
AluI AGCT 8 cut(s) 15, 59, 91, 169, 179, 206, 889, 951
Alw21I GWGCWC 2 cut(s) 582, 771
Alw26I GTCTC 3 cut(s) 181, 719, 888
AlwI GGATC 1 cut(s) 284
AoxI GGCC 4 cut(s) 34, 531, 675, 798
ApeKI GCWGC 2 cut(s) 344, 518
ApoI RAATTY 3 cut(s) 461, 803, 1008
AspS9I GGNCC 3 cut(s) 406, 419, 584
AsuC2I CCSGG 2 cut(s) 137, 812
AsuHPI GGTGA 7 cut(s) 43, 97, 267, 328, 373, 574, 959
AvaII GGWCC 3 cut(s) 406, 419, 584
BaeI ACNNNNGTAYC 2 cut(s) 739, 772
BalI TGGCCA 1 cut(s) 800
BbsI GAAGAC 1 cut(s) 367
Bbv12I GWGCWC 2 cut(s) 582, 771
BbvI GCAGC 2 cut(s) 356, 530
BccI CCATC 2 cut(s) 76, 248
BceAI ACGGC 2 cut(s) 49, 262
BciT130I CCWGG 2 cut(s) 415, 797
BcnI CCSGG 2 cut(s) 137, 812
BcoDI GTCTC 3 cut(s) 181, 719, 888
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 2 cut(s) 180, 735
BisI GCNGC 3 cut(s) 345, 348, 519
BlsI GCNGC 3 cut(s) 346, 349, 520
Bme1390I CCNGG 4 cut(s) 137, 415, 797, 812
Bme18I GGWCC 3 cut(s) 406, 419, 584
BmgT120I GGNCC 3 cut(s) 406, 419, 584
BmiI GGNNCC 5 cut(s) 124, 309, 325, 408, 420
BmrFI CCNGG 4 cut(s) 137, 415, 797, 812
BmrI ACTGGG 1 cut(s) 563
BmsI GCATC 2 cut(s) 281, 624
BmuI ACTGGG 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 367
BplI GAGNNNNNCTC 2 cut(s) 716, 748
BpmI CTGGAG 1 cut(s) 81
BpuEI CTTGAG 2 cut(s) 148, 614
BpuMI CCSGG 2 cut(s) 137, 812
BsaHI GRCGYC 2 cut(s) 696, 783
BsaJI CCNNGG 4 cut(s) 31, 357, 414, 775
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 5 cut(s) 415, 416, 483, 574, 737
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 6 cut(s) 488, 569, 646, 708, 917, 974
Bse3DI GCAATG 1 cut(s) 69
BseBI CCWGG 2 cut(s) 415, 797
BseDI CCNNGG 4 cut(s) 31, 357, 414, 775
BseGI GGATG 4 cut(s) 87, 145, 292, 624
BseLI CCNNNNNNNGG 5 cut(s) 415, 416, 483, 574, 737
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 6 cut(s) 488, 569, 646, 708, 917, 974
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 2 cut(s) 356, 530
BshFI GGCC 4 cut(s) 36, 533, 677, 800
BsiHKAI GWGCWC 2 cut(s) 582, 771
BsiSI CCGG 4 cut(s) 54, 137, 311, 812
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 5 cut(s) 415, 416, 483, 574, 737
BsmAI GTCTC 3 cut(s) 181, 719, 888
BsmFI GGGAC 1 cut(s) 707
BsnI GGCC 4 cut(s) 36, 533, 677, 800
Bsp1286I GDGCHC 2 cut(s) 582, 771
Bsp1407I TGTACA 1 cut(s) 399
Bsp143I GATC 3 cut(s) 208, 289, 454
BspACI CCGC 5 cut(s) 314, 347, 350, 395, 557
BspANI GGCC 4 cut(s) 36, 533, 677, 800
BspCNI CTCAG 1 cut(s) 751
BspLI GGNNCC 5 cut(s) 124, 309, 325, 408, 420
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 310
BsrGI TGTACA 1 cut(s) 399
BsrI ACTGG 6 cut(s) 488, 569, 646, 708, 917, 974
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 4 cut(s) 31, 357, 414, 775
BssMI GATC 3 cut(s) 208, 289, 454
BssNI GRCGYC 2 cut(s) 696, 783
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 2 cut(s) 415, 797
Bst4CI ACNGT 4 cut(s) 115, 604, 776, 943
BstACI GRCGYC 2 cut(s) 696, 783
BstAUI TGTACA 1 cut(s) 399
BstC8I GCNNGC 4 cut(s) 312, 516, 613, 953
BstDEI CTNAG 3 cut(s) 191, 759, 898
BstDSI CCRYGG 2 cut(s) 31, 775
BstEII GGTNACC 1 cut(s) 965
BstF5I GGATG 4 cut(s) 87, 145, 292, 624
BstKTI GATC 3 cut(s) 211, 292, 457
BstMAI GTCTC 3 cut(s) 181, 719, 888
BstMBI GATC 3 cut(s) 208, 289, 454
BstMWI GCNNNNNNNGC 2 cut(s) 353, 515
BstNI CCWGG 2 cut(s) 415, 797
BstNSI RCATGY 1 cut(s) 689
BstPI GGTNACC 1 cut(s) 965
BstSCI CCNGG 4 cut(s) 135, 413, 795, 810
BstSFI CTRYAG 2 cut(s) 180, 735
BstV1I GCAGC 2 cut(s) 356, 530
BstV2I GAAGAC 1 cut(s) 367
BsuRI GGCC 4 cut(s) 36, 533, 677, 800
BtgI CCRYGG 2 cut(s) 31, 775
BtsCI GGATG 4 cut(s) 87, 145, 292, 624
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 4 cut(s) 111, 510, 576, 701
Cac8I GCNNGC 4 cut(s) 312, 516, 613, 953
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 3 cut(s) 406, 419, 584
Csp6I GTAC 3 cut(s) 400, 487, 634
CviAII CATG 4 cut(s) 78, 403, 522, 686
CviQI GTAC 3 cut(s) 400, 487, 634
DdeI CTNAG 3 cut(s) 191, 759, 898
DpnI GATC 3 cut(s) 210, 291, 456
DpnII GATC 3 cut(s) 208, 289, 454
EaeI YGGCCR 2 cut(s) 34, 798
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 677
Eco47I GGWCC 3 cut(s) 406, 419, 584
Eco57I CTGAAG 1 cut(s) 444
Eco91I GGTNACC 1 cut(s) 965
EcoO109I RGGNCCY 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 965
EcoRII CCWGG 2 cut(s) 413, 795
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 4 cut(s) 81, 406, 525, 689
FaqI GGGAC 1 cut(s) 707
FatI CATG 4 cut(s) 77, 402, 521, 685
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 3 cut(s) 345, 348, 519
FokI GGATG 4 cut(s) 94, 152, 299, 611
Fsp4HI GCNGC 3 cut(s) 345, 348, 519
FspBI CTAG 1 cut(s) 98
GluI GCNGC 3 cut(s) 345, 348, 519
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 4 cut(s) 36, 533, 677, 800
HapII CCGG 4 cut(s) 54, 137, 311, 812
Hin1I GRCGYC 2 cut(s) 696, 783
Hin1II CATG 4 cut(s) 81, 406, 525, 689
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 3 cut(s) 337, 440, 998
HpaII CCGG 4 cut(s) 54, 137, 311, 812
HphI GGTGA 7 cut(s) 43, 97, 267, 328, 373, 574, 959
Hpy166II GTNNAC 7 cut(s) 319, 400, 436, 550, 565, 876, 912
Hpy188I TCNGA 2 cut(s) 28, 584
Hpy188III TCNNGA 4 cut(s) 293, 422, 458, 946
Hpy8I GTNNAC 7 cut(s) 319, 400, 436, 550, 565, 876, 912
Hpy99I CGWCG 4 cut(s) 440, 701, 785, 788
HpyAV CCTTC 3 cut(s) 167, 955, 980
HpyCH4III ACNGT 4 cut(s) 115, 604, 776, 943
HpyCH4IV ACGT 2 cut(s) 696, 783
HpyCH4V TGCA 6 cut(s) 201, 468, 595, 685, 737, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 515
HpyF3I CTNAG 3 cut(s) 191, 759, 898
HpySE526I ACGT 2 cut(s) 696, 783
Hsp92I GRCGYC 2 cut(s) 696, 783
Hsp92II CATG 4 cut(s) 81, 406, 525, 689
KroI GCCGGC 1 cut(s) 310
KroNI GCCGGC 1 cut(s) 312
Kzo9I GATC 3 cut(s) 208, 289, 454
LmnI GCTCC 5 cut(s) 56, 166, 176, 307, 774
Lsp1109I GCAGC 2 cut(s) 356, 530
LweI GCATC 2 cut(s) 281, 624
MaeI CTAG 1 cut(s) 98
MaeII ACGT 2 cut(s) 696, 783
MaeIII GTNAC 3 cut(s) 49, 280, 965
MalI GATC 3 cut(s) 210, 291, 456
MboI GATC 3 cut(s) 208, 289, 454
MboII GAAGA 2 cut(s) 367, 799
MfeI CAATTG 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 582, 771
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 5 cut(s) 387, 461, 664, 803, 1008
MluNI TGGCCA 1 cut(s) 800
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 3 cut(s) 51, 428, 885
MnlI CCTC 9 cut(s) 56, 98, 156, 239, 520, 523, 638, 734, 742
Mox20I TGGCCA 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 310
MscI TGGCCA 1 cut(s) 800
MseI TTAA 4 cut(s) 117, 243, 1005, 1018
Msp20I TGGCCA 1 cut(s) 800
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 4 cut(s) 54, 137, 311, 812
MspR9I CCNGG 4 cut(s) 137, 415, 797, 812
MunI CAATTG 1 cut(s) 387
MvaI CCWGG 2 cut(s) 415, 797
MwoI GCNNNNNNNGC 2 cut(s) 353, 515
NaeI GCCGGC 1 cut(s) 312
NciI CCSGG 2 cut(s) 137, 812
NdeII GATC 3 cut(s) 208, 289, 454
NgoMIV GCCGGC 1 cut(s) 310
NlaIII CATG 4 cut(s) 81, 406, 525, 689
NlaIV GGNNCC 5 cut(s) 124, 309, 325, 408, 420
NmuCI GTSAC 3 cut(s) 49, 280, 965
NspI RCATGY 1 cut(s) 689
PceI AGGCCT 1 cut(s) 677
PdiI GCCGGC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 337, 998
PflMI CCANNNNNTGG 1 cut(s) 483
PfoI TCCNGGA 1 cut(s) 810
PkrI GCNGC 3 cut(s) 346, 349, 520
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 413, 795
PspEI GGTNACC 1 cut(s) 965
PspGI CCWGG 2 cut(s) 413, 795
PspN4I GGNNCC 5 cut(s) 124, 309, 325, 408, 420
PspPI GGNCC 3 cut(s) 406, 419, 584
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 1 cut(s) 739
RsaI GTAC 3 cut(s) 401, 488, 635
RsaNI GTAC 3 cut(s) 400, 487, 634
SalI GTCGAC 1 cut(s) 434
SaqAI TTAA 4 cut(s) 117, 243, 1005, 1018
SatI GCNGC 3 cut(s) 345, 348, 519
Sau3AI GATC 3 cut(s) 208, 289, 454
Sau96I GGNCC 3 cut(s) 406, 419, 584
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 4 cut(s) 137, 415, 797, 812
SduI GDGCHC 2 cut(s) 582, 771
SfaNI GCATC 2 cut(s) 281, 624
SfcI CTRYAG 2 cut(s) 180, 735
SinI GGWCC 3 cut(s) 406, 419, 584
SmlI CTYRAG 2 cut(s) 127, 629
SmoI CTYRAG 2 cut(s) 127, 629
Sse9I AATT 5 cut(s) 387, 461, 664, 803, 1008
SseBI AGGCCT 1 cut(s) 677
SsiI CCGC 5 cut(s) 314, 347, 350, 395, 557
SspMI CTAG 1 cut(s) 98
StuI AGGCCT 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 135, 413, 795, 810
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 4 cut(s) 115, 604, 776, 943
TaiI ACGT 2 cut(s) 699, 786
TaqI TCGA 2 cut(s) 435, 647
TasI AATT 5 cut(s) 387, 461, 664, 803, 1008
TatI WGTACW 1 cut(s) 399
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 2 cut(s) 337, 998
Tru1I TTAA 4 cut(s) 117, 243, 1005, 1018
Tru9I TTAA 4 cut(s) 117, 243, 1005, 1018
TscAI CASTG 4 cut(s) 118, 510, 576, 708
TseFI GTSAC 3 cut(s) 49, 280, 965
TseI GCWGC 2 cut(s) 344, 518
Tsp45I GTSAC 3 cut(s) 49, 280, 965
TspDTI ATGAA 3 cut(s) 58, 66, 951
TspGWI ACGGA 2 cut(s) 299, 336
TspRI CASTG 4 cut(s) 118, 510, 576, 708
Van91I CCANNNNNTGG 1 cut(s) 483
VpaK11BI GGWCC 3 cut(s) 406, 419, 584
XapI RAATTY 3 cut(s) 461, 803, 1008
XceI RCATGY 1 cut(s) 689
XmiI GTMKAC 1 cut(s) 435
XspI CTAG 1 cut(s) 98
ZraI GACGTC 2 cut(s) 697, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.