RLG00000029221

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002169
Physical Location & Seq
Reverse (-)
34717396 .. 34721309
3914 bp
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UTR
Exon/CDS
Intron
RLM00000029221

Sequence Viewer

Length: 1014 bp
ATGTCTGATGATCAGAGATCCTCACTTTCCGGCAACGGTCAAATTGTGTGTGTTACTGGGGCCGGAGGCTACATCGCTTCTTGGATTGTCAAGCTCCTTCTGGAGAGAGGCTATCATGTGAGAGGAACTTTGAGAAACCCAGATGACCCAAAGAATGTTCATTTGAGAGAGCTGGAAGGAGCTAGAGAGAGGCTGAGCTTGAGAAAAGTCGATCTTCTCAATTTAGAGAGCCTTAAAGATGCCATTGCCGGCTGCGATGGCATTTTCCACACAGCATCCCCTACGACTGATGATCCGGAAGAAATGGTGTTGCCGGCTGTGAATGGAACAAAGAATGTGATTGTTGTCGCTTCTGAAACTAAGGTTAAACGCGTGGTCTTCACATCTTCAAATGGTGCCATCTACATGAACCCCACCAGAGGTCCCGATGTGGTTGTTGATGAGTCCTGTTGGAGTGACTTGGAGTTTTGCAAGAACACCAAGAACTGGTACTGCTATGGGAAAACTGTGGCAGAGCTGGCAGCTTGGGATGAAGCCAAAGAGAGAGGGGTGGACTTGGTTGTGGTGAACCCAGTTTATGTGCTTGGACCACTGCTCCAACCCACCATTAACACTAGCACTGTCCACATCCTCAAGTACTTGACTGGCTCAGCCAAGACTTATGCCAATTCAATTCAAGCCTATGTGCATGTTAAGGATGTGGCATTAGCACACATACTAGTGTATGAAACTCCCTCTGCCTCCGGTCGTTATCTTTGCGGCGAGAGCATTCTTCACCGTGGAGATGTGGTTGAAATCCTTGCCAAGTACTTCCCTGAATACCCAATACCTACCAGGTTGAAAGAAGATGGGAAACCTAGAGCAAAACCATACAAGTTCTCAAACCAGAAGCTTAAAGACCTGGGTTTGGAGTTCACCCCAGTGGAGCAGAGCCTATATGACACTGTTAAGAGCTTGCAGGACAAGGGGCACCTTAAAGTTCCTTCAAAACAAAAACACTCCTCCAAATTATGA

Protein Analysis

338

Amino Acids

37.32

Weight (kDa)

8.08

Isoelectric Point (pI)

28.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 15 - 130 4.9e-09 NmrA-like family
Epimerase PF01370 16 - 248 1.3e-24 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 16 - 138 5.7e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 18 - 241 4.1e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 18 - 253 1.1e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 20 - 133 2.2e-15 NAD(P)H-binding
NAD_binding_4 PF07993 76 - 206 7e-08 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 395, 969
AccB7I CCANNNNNTGG 1 cut(s) 486
AccII CGCG 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 1 cut(s) 759
AclWI GGATC 2 cut(s) 12, 287
AfaI GTAC 3 cut(s) 491, 638, 809
AfiI CCNNNNNNNGG 3 cut(s) 419, 486, 907
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 6 cut(s) 390, 672, 677, 794, 841, 987
AhlI ACTAGT 1 cut(s) 718
AjnI CCWGG 2 cut(s) 833, 900
AleI CACNNNNGTG 1 cut(s) 920
AluBI AGCT 8 cut(s) 94, 172, 182, 198, 517, 524, 892, 954
AluI AGCT 8 cut(s) 94, 172, 182, 198, 517, 524, 892, 954
AlwI GGATC 2 cut(s) 12, 287
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 2 cut(s) 252, 521
AspS9I GGNCC 3 cut(s) 60, 422, 587
AsuHPI GGTGA 3 cut(s) 577, 767, 907
AvaII GGWCC 2 cut(s) 422, 587
BaeGI GKGCMC 1 cut(s) 972
BanI GGYRCC 2 cut(s) 395, 969
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 239, 533
BccI CCATC 3 cut(s) 251, 407, 842
BciT130I CCWGG 2 cut(s) 835, 902
BclI TGATCA 1 cut(s) 10
BcuI ACTAGT 1 cut(s) 718
BfaI CTAG 4 cut(s) 183, 615, 719, 858
BisI GCNGC 3 cut(s) 253, 522, 760
BlpI GCTNAGC 2 cut(s) 194, 649
BlsI GCNGC 3 cut(s) 254, 523, 761
BmcAI AGTACT 2 cut(s) 638, 809
Bme1390I CCNGG 2 cut(s) 835, 902
Bme18I GGWCC 2 cut(s) 422, 587
BmgT120I GGNCC 3 cut(s) 60, 422, 587
BmiI GGNNCC 4 cut(s) 61, 397, 424, 971
BmrFI CCNGG 2 cut(s) 835, 902
BmrI ACTGGG 3 cut(s) 66, 566, 914
BmsI GCATC 2 cut(s) 229, 284
BmuI ACTGGG 3 cut(s) 66, 566, 914
BpiI GAAGAC 1 cut(s) 370
BpmI CTGGAG 1 cut(s) 122
Bpu1102I GCTNAGC 2 cut(s) 194, 649
BpuEI CTTGAG 2 cut(s) 220, 617
BsaJI CCNNGG 2 cut(s) 778, 901
BsaWI WCCGGW 2 cut(s) 295, 743
BsaXI ACNNNNNCTCC 4 cut(s) 579, 609, 774, 804
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 486, 907
Bse118I RCCGGY 2 cut(s) 248, 313
Bse1I ACTGG 5 cut(s) 61, 491, 572, 649, 920
Bse3DI GCAATG 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 2 cut(s) 835, 902
BseDI CCNNGG 2 cut(s) 778, 901
BseGI GGATG 4 cut(s) 275, 535, 627, 703
BseLI CCNNNNNNNGG 3 cut(s) 419, 486, 907
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 2 cut(s) 185, 663
BseNI ACTGG 5 cut(s) 61, 491, 572, 649, 920
BseRI GAGGAG 1 cut(s) 991
BseSI GKGCMC 1 cut(s) 972
BseXI GCAGC 2 cut(s) 239, 533
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 748
BshFI GGCC 1 cut(s) 62
BshNI GGYRCC 2 cut(s) 395, 969
BsiEI CGRYCG 1 cut(s) 748
BsiSI CCGG 6 cut(s) 30, 63, 249, 296, 314, 744
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 3 cut(s) 419, 486, 907
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 768
BsnI GGCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 972
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 4 cut(s) 10, 17, 211, 292
Bsp1720I GCTNAGC 2 cut(s) 194, 649
BspACI CCGC 1 cut(s) 759
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 2 cut(s) 186, 662
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 4 cut(s) 61, 397, 424, 971
BspPI GGATC 2 cut(s) 12, 287
BspT107I GGYRCC 2 cut(s) 395, 969
BsrDI GCAATG 1 cut(s) 243
BsrFI RCCGGY 2 cut(s) 248, 313
BsrI ACTGG 5 cut(s) 61, 491, 572, 649, 920
BssAI RCCGGY 2 cut(s) 248, 313
BssECI CCNNGG 2 cut(s) 778, 901
BssMI GATC 4 cut(s) 10, 17, 211, 292
Bst2UI CCWGG 2 cut(s) 835, 902
Bst4CI ACNGT 5 cut(s) 38, 508, 622, 779, 946
BstC8I GCNNGC 4 cut(s) 250, 315, 519, 956
BstDEI CTNAG 3 cut(s) 194, 360, 649
BstDSI CCRYGG 1 cut(s) 778
BstF5I GGATG 4 cut(s) 275, 535, 627, 703
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 4 cut(s) 13, 20, 214, 295
BstMBI GATC 4 cut(s) 10, 17, 211, 292
BstMCI CGRYCG 1 cut(s) 748
BstMWI GCNNNNNNNGC 3 cut(s) 258, 518, 765
BstNI CCWGG 2 cut(s) 835, 902
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 2 cut(s) 833, 900
BstSLI GKGCMC 1 cut(s) 972
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 239, 533
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 778
BtgZI GCGATG 2 cut(s) 58, 270
BtsCI GGATG 4 cut(s) 275, 535, 627, 703
BtsI GCAGTG 1 cut(s) 590
BtsIMutI CAGTG 4 cut(s) 590, 618, 927, 942
Cac8I GCNNGC 4 cut(s) 250, 315, 519, 956
Cfr10I RCCGGY 2 cut(s) 248, 313
Cfr13I GGNCC 3 cut(s) 60, 422, 587
CsiI ACCWGGT 1 cut(s) 833
Csp6I GTAC 3 cut(s) 490, 637, 808
CviAII CATG 3 cut(s) 116, 406, 689
CviQI GTAC 3 cut(s) 490, 637, 808
DdeI CTNAG 3 cut(s) 194, 360, 649
DpnI GATC 4 cut(s) 12, 19, 213, 294
DpnII GATC 4 cut(s) 10, 17, 211, 292
Eco47I GGWCC 2 cut(s) 422, 587
EcoO109I RGGNCCY 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 833, 900
FaeI CATG 3 cut(s) 119, 409, 692
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 408
FatI CATG 3 cut(s) 115, 405, 688
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 3 cut(s) 253, 522, 760
FokI GGATG 4 cut(s) 262, 542, 614, 710
Fsp4HI GCNGC 3 cut(s) 253, 522, 760
FspBI CTAG 4 cut(s) 183, 615, 719, 858
GluI GCNGC 3 cut(s) 253, 522, 760
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 1 cut(s) 62
HapII CCGG 6 cut(s) 30, 63, 249, 296, 314, 744
Hin1II CATG 3 cut(s) 119, 409, 692
HindIII AAGCTT 1 cut(s) 890
HinfI GANTC 1 cut(s) 443
HpaII CCGG 6 cut(s) 30, 63, 249, 296, 314, 744
HphI GGTGA 3 cut(s) 577, 767, 907
Hpy166II GTNNAC 4 cut(s) 553, 568, 625, 915
Hpy188I TCNGA 3 cut(s) 7, 15, 355
Hpy188III TCNNGA 3 cut(s) 101, 296, 425
Hpy8I GTNNAC 4 cut(s) 553, 568, 625, 915
HpyAV CCTTC 3 cut(s) 107, 170, 993
HpyCH4III ACNGT 5 cut(s) 38, 508, 622, 779, 946
HpyCH4V TGCA 3 cut(s) 471, 688, 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 518, 765
HpyF3I CTNAG 3 cut(s) 194, 360, 649
Hsp92II CATG 3 cut(s) 119, 409, 692
Kpn2I TCCGGA 1 cut(s) 295
KroI GCCGGC 2 cut(s) 248, 313
KroNI GCCGGC 2 cut(s) 250, 315
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 4 cut(s) 10, 17, 211, 292
LmnI GCTCC 4 cut(s) 99, 179, 600, 925
Lsp1109I GCAGC 2 cut(s) 239, 533
LweI GCATC 2 cut(s) 229, 284
MabI ACCWGGT 1 cut(s) 833
MaeI CTAG 4 cut(s) 183, 615, 719, 858
MaeIII GTNAC 2 cut(s) 52, 455
MalI GATC 4 cut(s) 12, 19, 213, 294
MboI GATC 4 cut(s) 10, 17, 211, 292
MboII GAAGA 6 cut(s) 206, 311, 370, 378, 764, 857
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 972
MluCI AATT 5 cut(s) 42, 220, 667, 672, 1007
MluI ACGCGT 1 cut(s) 370
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 2 cut(s) 431, 622
MroI TCCGGA 1 cut(s) 295
MroNI GCCGGC 2 cut(s) 248, 313
MseI TTAA 7 cut(s) 234, 366, 609, 693, 894, 948, 975
MslI CAYNNNNRTG 3 cut(s) 404, 719, 920
MspI CCGG 6 cut(s) 30, 63, 249, 296, 314, 744
MspR9I CCNGG 2 cut(s) 835, 902
Mva1269I GAATGC 1 cut(s) 768
MvaI CCWGG 2 cut(s) 835, 902
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 258, 518, 765
NaeI GCCGGC 2 cut(s) 250, 315
NdeII GATC 4 cut(s) 10, 17, 211, 292
NgoMIV GCCGGC 2 cut(s) 248, 313
NlaIII CATG 3 cut(s) 119, 409, 692
NlaIV GGNNCC 4 cut(s) 61, 397, 424, 971
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 1 cut(s) 692
OliI CACNNNNGTG 1 cut(s) 920
PctI GAATGC 1 cut(s) 768
PdiI GCCGGC 2 cut(s) 250, 315
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 3 cut(s) 254, 523, 761
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PpuMI RGGWCCY 1 cut(s) 422
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 2 cut(s) 833, 900
PspGI CCWGG 2 cut(s) 833, 900
PspN4I GGNNCC 4 cut(s) 61, 397, 424, 971
PspPI GGNCC 3 cut(s) 60, 422, 587
PspPPI RGGWCCY 1 cut(s) 422
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 3 cut(s) 491, 638, 809
RsaNI GTAC 3 cut(s) 490, 637, 808
RseI CAYNNNNRTG 3 cut(s) 404, 719, 920
SaqAI TTAA 7 cut(s) 234, 366, 609, 693, 894, 948, 975
SatI GCNGC 3 cut(s) 253, 522, 760
Sau3AI GATC 4 cut(s) 10, 17, 211, 292
Sau96I GGNCC 3 cut(s) 60, 422, 587
ScaI AGTACT 2 cut(s) 638, 809
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 835, 902
SduI GDGCHC 1 cut(s) 972
SexAI ACCWGGT 1 cut(s) 833
SfaNI GCATC 2 cut(s) 229, 284
SinI GGWCC 2 cut(s) 422, 587
SmiMI CAYNNNNRTG 3 cut(s) 404, 719, 920
SmlI CTYRAG 2 cut(s) 199, 632
SmoI CTYRAG 2 cut(s) 199, 632
SpeI ACTAGT 1 cut(s) 718
Sse9I AATT 5 cut(s) 42, 220, 667, 672, 1007
SsiI CCGC 1 cut(s) 759
SspMI CTAG 4 cut(s) 183, 615, 719, 858
StyD4I CCNGG 2 cut(s) 833, 900
TaaI ACNGT 5 cut(s) 38, 508, 622, 779, 946
TaqI TCGA 1 cut(s) 210
TasI AATT 5 cut(s) 42, 220, 667, 672, 1007
TatI WGTACW 2 cut(s) 636, 807
TauI GCSGC 1 cut(s) 762
Tru1I TTAA 7 cut(s) 234, 366, 609, 693, 894, 948, 975
Tru9I TTAA 7 cut(s) 234, 366, 609, 693, 894, 948, 975
TscAI CASTG 4 cut(s) 597, 625, 927, 949
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 2 cut(s) 252, 521
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 4 cut(s) 149, 422, 546, 741
TspRI CASTG 4 cut(s) 597, 625, 927, 949
Van91I CCANNNNNTGG 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 422, 587
XceI RCATGY 1 cut(s) 692
XspI CTAG 4 cut(s) 183, 615, 719, 858
ZrmI AGTACT 2 cut(s) 638, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.