MD17G1222400.v1.1

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002169
Physical Location & Seq
Forward (+)
27079686 .. 27085895
6210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1222400.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGCCTGCCGATAGCTCATCAGTTTCCGGCCACGGCCAAACTATTTGTGTCACTGGAGCTGGAGGCTTCATTGCTTCTTGGATGGTGAAGCTATTGCTCGAAAGAGGCTACACTGTTAGAGGAACCTTGAGAAACCCCGATGACCCAAAGAATGCTCATTTGAGAGAGCTTGAAGGAGCTGCAGAGAGACTAACTTTGTGCAGAGCTGATCTCCTTGATTATGACAGCCTCAAAGAAGCCATTAATGGCTGTGATGGAGTTTTTCACACAGCATCACCTGTCACGGATGATCCAGAACAAATGGTGGAGCCCGCGGTGAACGGAACCAAGAATGTGATTCAGGCAGCGGCGGAAGCCAAGGTGAAACGGGTGGTTTTCACGTCGTCAATCGGTGCGGTGTACATGGACCCCACCAGGGGTCCTGATGTGGTTGTTGATGAGTCTTGTTGGAGTGATCTTGAATTTTGCAAGAACACCAAGAACTGGTACTGCTATGGGAAAGCAGTGGCAGAGCAGGCAGCATGGGCTGAGGCCAAAGAGAAAGGGGTGGACGTGGTGGTAGTGAACCCAGTGCTGGTGCTCGGACCACTTCTGCAACCAACTGTCAACGCTAGCACCATTCACATCCTCAAGTACCTGACAGGCTCAACCCAGACATATGCCAATTCAGTTCAGGCCTATGTGCATGTTAGGGACGTCGCACTGGCTCACATTCTGGTCTATGAGACACCCTCTGCCTCCGGCAGATATCTTTGTGCTGAGAGTGTGCTCCACCGGGGCGACGTGGTGGAAATCCTGGCAAAATTCTTCCCGGAATACCCTATTCCCAACAAGTGTAAAGATAATGGGAAACCCAGAGCAGAACCATACAAGTTCACAAACCAGAAGCTACGAGACTTAGGGTTGGAGTTCACCCCAGTCAAACATACCCTGTATGAAACTGTCAAGAGCTTGCAGGAGAAGGGCCACCTTCCAGTCCCTACAAAACAAGAACAAGATTCCATTAAAATTCAATCTTAA

Protein Analysis

340

Amino Acids

37.18

Weight (kDa)

6.02

Isoelectric Point (pI)

32.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 13 - 132 1.1e-14 NmrA-like family
KR PF08659 14 - 137 1.4e-06 KR domain
Epimerase PF01370 15 - 247 9.2e-31 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 15 - 134 4.3e-08 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 17 - 240 5.9e-22 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 17 - 264 1.4e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 17 - 200 3.6e-12 Male sterility protein
NAD_binding_10 PF13460 19 - 135 2.7e-19 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 699
AccB7I CCANNNNNTGG 1 cut(s) 483
AccII CGCG 1 cut(s) 314
AciI CCGC 5 cut(s) 312, 314, 347, 350, 395
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 2 cut(s) 28, 34
AcsI RAATTY 3 cut(s) 461, 803, 1008
AcyI GRCGYC 1 cut(s) 696
AfaI GTAC 3 cut(s) 401, 488, 635
AfiI CCNNNNNNNGG 4 cut(s) 415, 416, 483, 574
AgsI TTSAA 3 cut(s) 173, 461, 1013
AjiI CACGTC 3 cut(s) 381, 553, 784
AjnI CCWGG 2 cut(s) 413, 795
AluBI AGCT 8 cut(s) 15, 59, 91, 169, 179, 206, 889, 951
AluI AGCT 8 cut(s) 15, 59, 91, 169, 179, 206, 889, 951
Alw21I GWGCWC 2 cut(s) 582, 771
Alw26I GTCTC 3 cut(s) 181, 719, 888
AlwI GGATC 1 cut(s) 284
AoxI GGCC 5 cut(s) 28, 34, 531, 675, 964
ApeKI GCWGC 3 cut(s) 179, 344, 518
ApoI RAATTY 3 cut(s) 461, 803, 1008
AseI ATTAAT 1 cut(s) 243
AspS9I GGNCC 4 cut(s) 406, 419, 584, 964
AsuC2I CCSGG 2 cut(s) 776, 812
AsuHPI GGTGA 5 cut(s) 97, 267, 328, 373, 904
AsuNHI GCTAGC 1 cut(s) 611
AvaII GGWCC 3 cut(s) 406, 419, 584
BanII GRGCYC 1 cut(s) 312
Bbv12I GWGCWC 2 cut(s) 582, 771
BbvCI CCTCAGC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 166, 356, 530
BccI CCATC 2 cut(s) 76, 248
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 2 cut(s) 415, 797
BcnI CCSGG 2 cut(s) 776, 812
BcoDI GTCTC 3 cut(s) 181, 719, 888
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 1 cut(s) 180
BisI GCNGC 4 cut(s) 180, 345, 348, 519
BlsI GCNGC 4 cut(s) 181, 346, 349, 520
Bme1390I CCNGG 4 cut(s) 415, 776, 797, 812
Bme18I GGWCC 3 cut(s) 406, 419, 584
BmgBI CACGTC 3 cut(s) 381, 553, 784
BmgT120I GGNCC 4 cut(s) 406, 419, 584, 964
BmiI GGNNCC 5 cut(s) 124, 309, 325, 408, 420
BmrFI CCNGG 4 cut(s) 415, 776, 797, 812
BmrI ACTGGG 2 cut(s) 563, 911
BmsI GCATC 1 cut(s) 281
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 2 cut(s) 563, 911
BplI GAGNNNNNCTC 4 cut(s) 195, 227, 716, 748
BpmI CTGGAG 2 cut(s) 75, 81
Bpu10I CCTNAGC 1 cut(s) 528
BpuEI CTTGAG 2 cut(s) 148, 614
BpuMI CCSGG 2 cut(s) 776, 812
BsaHI GRCGYC 1 cut(s) 696
BsaJI CCNNGG 5 cut(s) 31, 312, 357, 414, 775
Bsc4I CCNNNNNNNGG 4 cut(s) 415, 416, 483, 574
Bse1I ACTGG 6 cut(s) 58, 488, 569, 708, 917, 974
Bse3DI GCAATG 1 cut(s) 69
BseBI CCWGG 2 cut(s) 415, 797
BseDI CCNNGG 5 cut(s) 31, 312, 357, 414, 775
BseGI GGATG 3 cut(s) 87, 292, 624
BseLI CCNNNNNNNGG 4 cut(s) 415, 416, 483, 574
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 2 cut(s) 519, 750
BseNI ACTGG 6 cut(s) 58, 488, 569, 708, 917, 974
BseXI GCAGC 3 cut(s) 166, 356, 530
BsgI GTGCAG 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 314
BshFI GGCC 5 cut(s) 30, 36, 533, 677, 966
BsiHKAI GWGCWC 2 cut(s) 582, 771
BsiSI CCGG 4 cut(s) 27, 741, 775, 812
BslFI GGGAC 2 cut(s) 707, 962
BslI CCNNNNNNNGG 4 cut(s) 415, 416, 483, 574
BsmAI GTCTC 3 cut(s) 181, 719, 888
BsmFI GGGAC 2 cut(s) 707, 962
BsmI GAATGC 1 cut(s) 157
BsnI GGCC 5 cut(s) 30, 36, 533, 677, 966
Bsp1286I GDGCHC 3 cut(s) 312, 582, 771
Bsp1407I TGTACA 1 cut(s) 399
Bsp143I GATC 3 cut(s) 208, 289, 454
BspACI CCGC 5 cut(s) 312, 314, 347, 350, 395
BspANI GGCC 5 cut(s) 30, 36, 533, 677, 966
BspCNI CTCAG 2 cut(s) 520, 751
BspFNI CGCG 1 cut(s) 314
BspLI GGNNCC 5 cut(s) 124, 309, 325, 408, 420
BspMAI CTGCAG 1 cut(s) 184
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 69
BsrGI TGTACA 1 cut(s) 399
BsrI ACTGG 6 cut(s) 58, 488, 569, 708, 917, 974
BssECI CCNNGG 5 cut(s) 31, 312, 357, 414, 775
BssMI GATC 3 cut(s) 208, 289, 454
BssNI GRCGYC 1 cut(s) 696
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 2 cut(s) 415, 797
Bst4CI ACNGT 3 cut(s) 115, 604, 943
BstACI GRCGYC 1 cut(s) 696
BstAUI TGTACA 1 cut(s) 399
BstC8I GCNNGC 5 cut(s) 6, 312, 516, 613, 953
BstDEI CTNAG 3 cut(s) 528, 759, 898
BstDSI CCRYGG 2 cut(s) 31, 312
BstF5I GGATG 3 cut(s) 87, 292, 624
BstFNI CGCG 1 cut(s) 314
BstKTI GATC 3 cut(s) 211, 292, 457
BstMAI GTCTC 3 cut(s) 181, 719, 888
BstMBI GATC 3 cut(s) 208, 289, 454
BstMWI GCNNNNNNNGC 3 cut(s) 353, 515, 524
BstNI CCWGG 2 cut(s) 415, 797
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 4 cut(s) 413, 774, 795, 810
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 166, 356, 530
BsuRI GGCC 5 cut(s) 30, 36, 533, 677, 966
BtgI CCRYGG 2 cut(s) 31, 312
BtrI CACGTC 3 cut(s) 381, 553, 784
BtsCI GGATG 3 cut(s) 87, 292, 624
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 5 cut(s) 51, 111, 510, 576, 701
Cac8I GCNNGC 5 cut(s) 6, 312, 516, 613, 953
Cfr13I GGNCC 4 cut(s) 406, 419, 584, 964
Cfr42I CCGCGG 1 cut(s) 315
Csp6I GTAC 3 cut(s) 400, 487, 634
CviAII CATG 3 cut(s) 403, 522, 686
CviQI GTAC 3 cut(s) 400, 487, 634
DdeI CTNAG 3 cut(s) 528, 759, 898
DpnI GATC 3 cut(s) 210, 291, 456
DpnII GATC 3 cut(s) 208, 289, 454
EaeI YGGCCR 2 cut(s) 28, 34
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 677
Eco24I GRGCYC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 749
Eco47I GGWCC 3 cut(s) 406, 419, 584
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 413, 795
EcoRV GATATC 1 cut(s) 749
EcoT14I CCWWGG 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 3 cut(s) 406, 525, 689
FaqI GGGAC 2 cut(s) 707, 962
FatI CATG 3 cut(s) 402, 521, 685
FauI CCCGC 1 cut(s) 319
FauNDI CATATG 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 180, 345, 348, 519
FokI GGATG 3 cut(s) 94, 299, 611
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 4 cut(s) 180, 345, 348, 519
FspBI CTAG 1 cut(s) 612
GluI GCNGC 4 cut(s) 180, 345, 348, 519
GsuI CTGGAG 2 cut(s) 75, 81
HaeIII GGCC 5 cut(s) 30, 36, 533, 677, 966
HapII CCGG 4 cut(s) 27, 741, 775, 812
Hin1I GRCGYC 1 cut(s) 696
Hin1II CATG 3 cut(s) 406, 525, 689
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HinfI GANTC 3 cut(s) 337, 440, 998
HpaII CCGG 4 cut(s) 27, 741, 775, 812
HphI GGTGA 5 cut(s) 97, 267, 328, 373, 904
Hpy166II GTNNAC 7 cut(s) 319, 400, 550, 565, 607, 876, 912
Hpy188I TCNGA 1 cut(s) 584
Hpy188III TCNNGA 4 cut(s) 293, 422, 458, 946
Hpy8I GTNNAC 7 cut(s) 319, 400, 550, 565, 607, 876, 912
Hpy99I CGWCG 3 cut(s) 385, 701, 785
HpyAV CCTTC 3 cut(s) 167, 955, 980
HpyCH4III ACNGT 3 cut(s) 115, 604, 943
HpyCH4IV ACGT 4 cut(s) 380, 552, 696, 783
HpyCH4V TGCA 6 cut(s) 182, 201, 468, 595, 685, 955
HpyF10VI GCNNNNNNNGC 3 cut(s) 353, 515, 524
HpyF3I CTNAG 3 cut(s) 528, 759, 898
HpySE526I ACGT 4 cut(s) 380, 552, 696, 783
Hsp92I GRCGYC 1 cut(s) 696
Hsp92II CATG 3 cut(s) 406, 525, 689
KspI CCGCGG 1 cut(s) 315
Kzo9I GATC 3 cut(s) 208, 289, 454
LmnI GCTCC 4 cut(s) 56, 176, 307, 774
Lsp1109I GCAGC 3 cut(s) 166, 356, 530
LweI GCATC 1 cut(s) 281
MaeI CTAG 1 cut(s) 612
MaeII ACGT 4 cut(s) 380, 552, 696, 783
MaeIII GTNAC 2 cut(s) 49, 280
MalI GATC 3 cut(s) 210, 291, 456
MboI GATC 3 cut(s) 208, 289, 454
MboII GAAGA 1 cut(s) 799
MhlI GDGCHC 3 cut(s) 312, 582, 771
MluCI AATT 4 cut(s) 461, 664, 803, 1008
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 428, 885
MnlI CCTC 8 cut(s) 56, 98, 113, 239, 523, 638, 742, 748
MseI TTAA 3 cut(s) 243, 1005, 1018
MspA1I CMGCKG 2 cut(s) 314, 347
MspI CCGG 4 cut(s) 27, 741, 775, 812
MspR9I CCNGG 4 cut(s) 415, 776, 797, 812
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 2 cut(s) 415, 797
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 3 cut(s) 353, 515, 524
NciI CCSGG 2 cut(s) 776, 812
NdeI CATATG 1 cut(s) 658
NdeII GATC 3 cut(s) 208, 289, 454
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 3 cut(s) 406, 525, 689
NlaIV GGNNCC 5 cut(s) 124, 309, 325, 408, 420
NmuCI GTSAC 2 cut(s) 49, 280
NspI RCATGY 1 cut(s) 689
PceI AGGCCT 1 cut(s) 677
PctI GAATGC 1 cut(s) 157
PfeI GAWTC 2 cut(s) 337, 998
PflMI CCANNNNNTGG 1 cut(s) 483
PfoI TCCNGGA 1 cut(s) 810
PkrI GCNGC 4 cut(s) 181, 346, 349, 520
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 419
PshBI ATTAAT 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 413, 795
PspGI CCWGG 2 cut(s) 413, 795
PspN4I GGNNCC 5 cut(s) 124, 309, 325, 408, 420
PspPI GGNCC 4 cut(s) 406, 419, 584, 964
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 1 cut(s) 184
RsaI GTAC 3 cut(s) 401, 488, 635
RsaNI GTAC 3 cut(s) 400, 487, 634
SacII CCGCGG 1 cut(s) 315
SaqAI TTAA 3 cut(s) 243, 1005, 1018
SatI GCNGC 4 cut(s) 180, 345, 348, 519
Sau3AI GATC 3 cut(s) 208, 289, 454
Sau96I GGNCC 4 cut(s) 406, 419, 584, 964
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 4 cut(s) 415, 776, 797, 812
SduI GDGCHC 3 cut(s) 312, 582, 771
SfaNI GCATC 1 cut(s) 281
SfcI CTRYAG 1 cut(s) 180
Sfr303I CCGCGG 1 cut(s) 315
SgrBI CCGCGG 1 cut(s) 315
SinI GGWCC 3 cut(s) 406, 419, 584
SmlI CTYRAG 2 cut(s) 127, 629
SmoI CTYRAG 2 cut(s) 127, 629
Sse9I AATT 4 cut(s) 461, 664, 803, 1008
SseBI AGGCCT 1 cut(s) 677
SsiI CCGC 5 cut(s) 312, 314, 347, 350, 395
SspMI CTAG 1 cut(s) 612
StuI AGGCCT 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 413, 774, 795, 810
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 3 cut(s) 115, 604, 943
TaiI ACGT 4 cut(s) 383, 555, 699, 786
TaqI TCGA 1 cut(s) 99
TasI AATT 4 cut(s) 461, 664, 803, 1008
TatI WGTACW 1 cut(s) 399
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 2 cut(s) 337, 998
Tru1I TTAA 3 cut(s) 243, 1005, 1018
Tru9I TTAA 3 cut(s) 243, 1005, 1018
TscAI CASTG 5 cut(s) 58, 118, 510, 576, 708
TseFI GTSAC 2 cut(s) 49, 280
TseI GCWGC 3 cut(s) 179, 344, 518
Tsp45I GTSAC 2 cut(s) 49, 280
TspDTI ATGAA 2 cut(s) 58, 951
TspGWI ACGGA 2 cut(s) 299, 336
TspRI CASTG 5 cut(s) 58, 118, 510, 576, 708
Van91I CCANNNNNTGG 1 cut(s) 483
VpaK11BI GGWCC 3 cut(s) 406, 419, 584
VspI ATTAAT 1 cut(s) 243
XapI RAATTY 3 cut(s) 461, 803, 1008
XceI RCATGY 1 cut(s) 689
XspI CTAG 1 cut(s) 612
ZraI GACGTC 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.