Rw1G013480

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002169
Physical Location & Seq
Reverse (-)
30112591 .. 30116538
3948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G013480.1

Sequence Viewer

Length: 1014 bp
ATGTCTGATGATCAGAGATCCTCACTTTCTGGCAACGGCCAAACTGTGTGTGTTACTGGGGCCGGAGGCTACATCGCTTCTTGGATTGTCAAGCTCCTTCTGGAGAGAGGCTATCATGTGAGAGGAACTTCGAGAAACCCAGATGACCCAAAGAATGTTCATTTGAGAGAGCTGGAAGGAGCCAGAGAGAGGCTGAGCTTGAGAAAAGTCGATCTTCTCGATTTAGAGAGCCTTAAAGATGCCATTGCCGGCTGCGATGGCATTTTCCACACAGCGTCCCCTTTAACTAATGATCCGGAAGAAATGGTGTCGCCGGCTGTGAATGGAATAAAGAATGTGATCGTTGCCGCTTCTGAAGCTAAGGTTAAACGCGTGGTCTTCACATCTTCAAACGGTGCCGTCTACATGAACCCCACCAGAGGTCCCGATGTGGTTGTCGATGAGTCCTGTTGGAGCGACCTGGAGTTTTGCAAGAACACCAAGAACTGGTACTGCTATGGCAAAACTGTGGCAGAGCTAGCAGCTTGGGATGAAGCCAAAGAGAGAGGGGTGGACTTGGTTGTGGTGAACCCAGTTTATGTGCTTGGACCACTGCTCCAACCCACCATCAACATTAGCACTGTCCACATCCTCAAGTACTTGACTGGCTCAGCCAAGACTTATGCCAATTCAATTCAGGCCTATGTGCATGTTAAGGATGTGGCATTAGCACACATACTAGTGTATGAAACTCCCTCTGCCTCCGGTCGTTATCTTTGCGGCGAGAGCGTCCTTCACCGTGGAGATGTGGTTGAAATCCTTGCCAAGTACTTCCCTGAATACCCAATACCCACCAAGATGAAAGAAGATGGGAAACCTAGAGCAAAACCATACAAGTTCTCAAACCAGAAGCTTAAAGACTTGGGTTTGGAGTTCACCCCAGTGAAGCAGAGCCTATATGACACTGTCAAGAGCTTGCAGGACAAGGGGCACCTTAAAGTTCCTACAAAACAAAAACACTCCGCCAAATTATGA

Protein Analysis

337

Amino Acids

37.19

Weight (kDa)

8.55

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 14 - 133 9.1e-12 NmrA-like family
Epimerase PF01370 16 - 248 3e-25 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 16 - 139 2e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 18 - 241 1.1e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 18 - 266 4.9e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 20 - 135 1.7e-15 NAD(P)H-binding
NAD_binding_4 PF07993 75 - 200 4.2e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 395, 969
AccB7I CCANNNNNTGG 1 cut(s) 486
AccI GTMKAC 1 cut(s) 402
AccII CGCG 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 3 cut(s) 348, 759, 1002
AclWI GGATC 2 cut(s) 12, 287
AcoI YGGCCR 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 3 cut(s) 491, 638, 809
AfiI CCNNNNNNNGG 3 cut(s) 189, 419, 486
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 390, 672, 794
AhlI ACTAGT 1 cut(s) 718
AjnI CCWGG 1 cut(s) 459
AleI CACNNNNGTG 1 cut(s) 920
AluBI AGCT 8 cut(s) 94, 172, 198, 359, 517, 524, 892, 954
AluI AGCT 8 cut(s) 94, 172, 198, 359, 517, 524, 892, 954
AlwI GGATC 2 cut(s) 12, 287
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 3 cut(s) 37, 60, 678
ApeKI GCWGC 2 cut(s) 252, 521
AspS9I GGNCC 3 cut(s) 60, 422, 587
AsuHPI GGTGA 3 cut(s) 577, 767, 907
AsuNHI GCTAGC 1 cut(s) 517
AvaII GGWCC 2 cut(s) 422, 587
BaeGI GKGCMC 1 cut(s) 972
BanI GGYRCC 2 cut(s) 395, 969
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 239, 533
BccI CCATC 3 cut(s) 251, 614, 842
BceAI ACGGC 2 cut(s) 52, 383
BciT130I CCWGG 1 cut(s) 461
BclI TGATCA 1 cut(s) 10
BcuI ACTAGT 1 cut(s) 718
BfaI CTAG 3 cut(s) 518, 719, 858
BisI GCNGC 4 cut(s) 253, 348, 522, 760
BlpI GCTNAGC 2 cut(s) 194, 649
BlsI GCNGC 4 cut(s) 254, 349, 523, 761
BmcAI AGTACT 2 cut(s) 638, 809
Bme1390I CCNGG 1 cut(s) 461
Bme18I GGWCC 2 cut(s) 422, 587
BmgT120I GGNCC 3 cut(s) 60, 422, 587
BmiI GGNNCC 5 cut(s) 61, 181, 397, 424, 971
BmrFI CCNGG 1 cut(s) 461
BmrI ACTGGG 3 cut(s) 66, 566, 914
BmsI GCATC 1 cut(s) 229
BmtI GCTAGC 1 cut(s) 521
BmuI ACTGGG 3 cut(s) 66, 566, 914
BpiI GAAGAC 1 cut(s) 370
BpmI CTGGAG 2 cut(s) 122, 482
Bpu10I CCTNAGC 1 cut(s) 360
Bpu1102I GCTNAGC 2 cut(s) 194, 649
BpuEI CTTGAG 2 cut(s) 220, 617
BsaJI CCNNGG 1 cut(s) 778
BsaWI WCCGGW 2 cut(s) 295, 743
BsaXI ACNNNNNCTCC 4 cut(s) 579, 609, 774, 804
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 419, 486
Bse118I RCCGGY 2 cut(s) 248, 313
Bse1I ACTGG 5 cut(s) 61, 491, 572, 649, 920
Bse3DI GCAATG 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 1 cut(s) 778
BseGI GGATG 3 cut(s) 535, 627, 703
BseLI CCNNNNNNNGG 3 cut(s) 189, 419, 486
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 2 cut(s) 185, 663
BseNI ACTGG 5 cut(s) 61, 491, 572, 649, 920
BseSI GKGCMC 1 cut(s) 972
BseXI GCAGC 2 cut(s) 239, 533
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 748
BshFI GGCC 3 cut(s) 39, 62, 680
BshNI GGYRCC 2 cut(s) 395, 969
BsiEI CGRYCG 1 cut(s) 748
BsiSI CCGG 5 cut(s) 63, 249, 296, 314, 744
BslFI GGGAC 2 cut(s) 262, 408
BslI CCNNNNNNNGG 3 cut(s) 189, 419, 486
BsmFI GGGAC 2 cut(s) 262, 408
BsnI GGCC 3 cut(s) 39, 62, 680
Bsp1286I GDGCHC 1 cut(s) 972
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 5 cut(s) 10, 17, 211, 292, 339
Bsp1720I GCTNAGC 2 cut(s) 194, 649
BspACI CCGC 3 cut(s) 348, 759, 1002
BspANI GGCC 3 cut(s) 39, 62, 680
BspCNI CTCAG 2 cut(s) 186, 662
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 5 cut(s) 61, 181, 397, 424, 971
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 2 cut(s) 12, 287
BspT107I GGYRCC 2 cut(s) 395, 969
BsrDI GCAATG 1 cut(s) 243
BsrFI RCCGGY 2 cut(s) 248, 313
BsrI ACTGG 5 cut(s) 61, 491, 572, 649, 920
BssAI RCCGGY 2 cut(s) 248, 313
BssECI CCNNGG 1 cut(s) 778
BssMI GATC 5 cut(s) 10, 17, 211, 292, 339
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 6 cut(s) 46, 395, 508, 622, 779, 946
BstC8I GCNNGC 4 cut(s) 250, 315, 519, 956
BstDEI CTNAG 3 cut(s) 194, 360, 649
BstDSI CCRYGG 1 cut(s) 778
BstF5I GGATG 3 cut(s) 535, 627, 703
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 5 cut(s) 13, 20, 214, 295, 342
BstMBI GATC 5 cut(s) 10, 17, 211, 292, 339
BstMCI CGRYCG 1 cut(s) 748
BstMWI GCNNNNNNNGC 4 cut(s) 258, 356, 518, 765
BstNI CCWGG 1 cut(s) 461
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 972
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 239, 533
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 3 cut(s) 39, 62, 680
BtgI CCRYGG 1 cut(s) 778
BtgZI GCGATG 2 cut(s) 58, 270
BtsCI GGATG 3 cut(s) 535, 627, 703
BtsI GCAGTG 1 cut(s) 590
BtsIMutI CAGTG 4 cut(s) 590, 618, 927, 942
Cac8I GCNNGC 4 cut(s) 250, 315, 519, 956
Cfr10I RCCGGY 2 cut(s) 248, 313
Cfr13I GGNCC 3 cut(s) 60, 422, 587
CseI GACGC 2 cut(s) 264, 757
Csp6I GTAC 3 cut(s) 490, 637, 808
CviAII CATG 3 cut(s) 116, 406, 689
CviQI GTAC 3 cut(s) 490, 637, 808
DdeI CTNAG 3 cut(s) 194, 360, 649
DpnI GATC 5 cut(s) 12, 19, 213, 294, 341
DpnII GATC 5 cut(s) 10, 17, 211, 292, 339
EaeI YGGCCR 1 cut(s) 37
EciI GGCGGA 1 cut(s) 991
Eco147I AGGCCT 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 422, 587
Eco57I CTGAAG 1 cut(s) 375
EcoO109I RGGNCCY 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 459
FaeI CATG 3 cut(s) 119, 409, 692
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 2 cut(s) 262, 408
FatI CATG 3 cut(s) 115, 405, 688
FbaI TGATCA 1 cut(s) 10
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 253, 348, 522, 760
FokI GGATG 3 cut(s) 542, 614, 710
Fsp4HI GCNGC 4 cut(s) 253, 348, 522, 760
FspBI CTAG 3 cut(s) 518, 719, 858
GluI GCNGC 4 cut(s) 253, 348, 522, 760
GsuI CTGGAG 2 cut(s) 122, 482
HaeIII GGCC 3 cut(s) 39, 62, 680
HapII CCGG 5 cut(s) 63, 249, 296, 314, 744
HgaI GACGC 2 cut(s) 264, 757
Hin1II CATG 3 cut(s) 119, 409, 692
HindIII AAGCTT 1 cut(s) 890
HinfI GANTC 1 cut(s) 443
HpaII CCGG 5 cut(s) 63, 249, 296, 314, 744
HphI GGTGA 3 cut(s) 577, 767, 907
Hpy166II GTNNAC 5 cut(s) 403, 553, 568, 625, 915
Hpy188I TCNGA 3 cut(s) 7, 15, 355
Hpy188III TCNNGA 6 cut(s) 101, 132, 218, 296, 425, 949
Hpy8I GTNNAC 5 cut(s) 403, 553, 568, 625, 915
HpyAV CCTTC 3 cut(s) 107, 170, 782
HpyCH4III ACNGT 6 cut(s) 46, 395, 508, 622, 779, 946
HpyCH4V TGCA 3 cut(s) 471, 688, 958
HpyF10VI GCNNNNNNNGC 4 cut(s) 258, 356, 518, 765
HpyF3I CTNAG 3 cut(s) 194, 360, 649
Hsp92II CATG 3 cut(s) 119, 409, 692
Kpn2I TCCGGA 1 cut(s) 295
KroI GCCGGC 2 cut(s) 248, 313
KroNI GCCGGC 2 cut(s) 250, 315
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 5 cut(s) 10, 17, 211, 292, 339
LmnI GCTCC 4 cut(s) 99, 179, 453, 600
Lsp1109I GCAGC 2 cut(s) 239, 533
LweI GCATC 1 cut(s) 229
MaeI CTAG 3 cut(s) 518, 719, 858
MaeIII GTNAC 1 cut(s) 52
MalI GATC 5 cut(s) 12, 19, 213, 294, 341
MboI GATC 5 cut(s) 10, 17, 211, 292, 339
MboII GAAGA 5 cut(s) 206, 311, 370, 378, 857
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 972
MluCI AATT 3 cut(s) 667, 672, 1007
MluI ACGCGT 1 cut(s) 370
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 2 cut(s) 431, 622
MroI TCCGGA 1 cut(s) 295
MroNI GCCGGC 2 cut(s) 248, 313
MseI TTAA 6 cut(s) 234, 284, 366, 693, 894, 975
MslI CAYNNNNRTG 3 cut(s) 719, 836, 920
MspI CCGG 5 cut(s) 63, 249, 296, 314, 744
MspR9I CCNGG 1 cut(s) 461
MvaI CCWGG 1 cut(s) 461
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 4 cut(s) 258, 356, 518, 765
NaeI GCCGGC 2 cut(s) 250, 315
NdeII GATC 5 cut(s) 10, 17, 211, 292, 339
NgoMIV GCCGGC 2 cut(s) 248, 313
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 3 cut(s) 119, 409, 692
NlaIV GGNNCC 5 cut(s) 61, 181, 397, 424, 971
NspI RCATGY 1 cut(s) 692
OliI CACNNNNGTG 1 cut(s) 920
PceI AGGCCT 1 cut(s) 680
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdiI GCCGGC 2 cut(s) 250, 315
PflFI GACNNNGTC 1 cut(s) 944
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 4 cut(s) 254, 349, 523, 761
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PpuMI RGGWCCY 1 cut(s) 422
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 459
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 5 cut(s) 61, 181, 397, 424, 971
PspPI GGNCC 3 cut(s) 60, 422, 587
PspPPI RGGWCCY 1 cut(s) 422
PsuI RGATCY 1 cut(s) 17
PsyI GACNNNGTC 1 cut(s) 944
RsaI GTAC 3 cut(s) 491, 638, 809
RsaNI GTAC 3 cut(s) 490, 637, 808
RseI CAYNNNNRTG 3 cut(s) 719, 836, 920
SaqAI TTAA 6 cut(s) 234, 284, 366, 693, 894, 975
SatI GCNGC 4 cut(s) 253, 348, 522, 760
Sau3AI GATC 5 cut(s) 10, 17, 211, 292, 339
Sau96I GGNCC 3 cut(s) 60, 422, 587
ScaI AGTACT 2 cut(s) 638, 809
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 1 cut(s) 461
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 1 cut(s) 229
SinI GGWCC 2 cut(s) 422, 587
SmiMI CAYNNNNRTG 3 cut(s) 719, 836, 920
SmlI CTYRAG 2 cut(s) 199, 632
SmoI CTYRAG 2 cut(s) 199, 632
SpeI ACTAGT 1 cut(s) 718
Sse9I AATT 3 cut(s) 667, 672, 1007
SseBI AGGCCT 1 cut(s) 680
SsiI CCGC 3 cut(s) 348, 759, 1002
SspMI CTAG 3 cut(s) 518, 719, 858
StuI AGGCCT 1 cut(s) 680
StyD4I CCNGG 1 cut(s) 459
TaaI ACNGT 6 cut(s) 46, 395, 508, 622, 779, 946
TaqI TCGA 4 cut(s) 131, 210, 219, 438
TasI AATT 3 cut(s) 667, 672, 1007
TatI WGTACW 2 cut(s) 636, 807
TauI GCSGC 2 cut(s) 350, 762
Tru1I TTAA 6 cut(s) 234, 284, 366, 693, 894, 975
Tru9I TTAA 6 cut(s) 234, 284, 366, 693, 894, 975
TscAI CASTG 4 cut(s) 597, 625, 927, 949
TseI GCWGC 2 cut(s) 252, 521
TspDTI ATGAA 5 cut(s) 149, 422, 546, 741, 854
TspRI CASTG 4 cut(s) 597, 625, 927, 949
Tth111I GACNNNGTC 1 cut(s) 944
Van91I CCANNNNNTGG 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 422, 587
XceI RCATGY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 402
XspI CTAG 3 cut(s) 518, 719, 858
ZrmI AGTACT 2 cut(s) 638, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.