RLG00000029205

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002169
Physical Location & Seq
Forward (+)
34452143 .. 34456076
3934 bp
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UTR
Exon/CDS
Intron
RLM00000029205

Sequence Viewer

Length: 1014 bp
ATGTTTGATGATCAGAGATCCTCAGTTTCTAGCAACAACCAAACTGTGTGTGTTACTGGGGCTGGAGGCTTCATTGCTTCTTGGATTGTCAAGCTCCTTCTGGAAAGAGGCTATCATGTGAGAGGAACTTTGAGAAACCCAGATGACCCAAAGAATGTTCATTTGAGAGAGCTGGAAGGAGCCAGAGAGAGGCTAAGCTTGAGAAAAGTCGATCTTCTCGATTTAGAGAGCCTTAAAGATGCCATTGCTGGCTGCGATGGCATTTTCCACACAGCATCCCCTCTAACTGATGATCCGGAAGAAATGGTGTTACCGGCTGTGAATGGAACAAAGAATGTGATCGTTGCCGCTTCTGAAGCTAAGGTTAAACGCGTGGTCTTCACATCTTCAATCGGTGCCGTCCACATGAACCCCACCAGAGGTCCCGATGTGGTTGTCGATGAGTCCTGGTGGAGTGACCTGGAGTTTTGCAAGAACACCAAGAACTGGTACTGCTATGGGAAAACTGCAGCAGAGCTAGCAGCGTGGGATGAAGCCAAAGAGAGAGGGGTGGACTTGGTTGTGGTGATCCCAGTTTTTGTGCTTGGACCGCTACTCCAACCCACCATCAACACTAGCACTGTCCATATCCTCAAGTACTTGACTGGCTCAGCCAAGACTTATGCCAATTCAATTCAAGCCTATGTGCATGTTAAGGATGTGGCATTAGCACACATAATGGTGTATGAACCTCCCTCTGCCTCCGGTCGTTATCTTTGCGGCGAGACTGTCCTTCACCGTGGAGATGTGGTTGAAATCCTTGCCAAGTACTTCCCTGAATACCCAATACCCACCAAGTTGAAAGAAGATGGGAAACCTAGAGCAAAACCATACAAGTTCTCAAACCAGAAGCTTAAAGACTTGGGTTTGGAGTTCACCCCAGTGGAGCAGAGCCTATATGACACTGTCAAGAGCTTGCAGGACAAGGGGCACCTTAAAGTTCCTACAAAACAAAAACACTCCGCCAAATTATGA

Protein Analysis

338

Amino Acids

37.36

Weight (kDa)

7.66

Isoelectric Point (pI)

24.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 13 - 143 4.9e-12 NmrA-like family
Epimerase PF01370 16 - 247 2.1e-24 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 16 - 135 3.5e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 18 - 240 1.5e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 18 - 130 6.2e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 20 - 140 1.8e-17 NAD(P)H-binding
NAD_binding_4 PF07993 75 - 205 6.1e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 395, 969
AccB7I CCANNNNNTGG 1 cut(s) 486
AccII CGCG 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 4 cut(s) 348, 590, 759, 1002
AclWI GGATC 3 cut(s) 12, 287, 562
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 3 cut(s) 491, 638, 809
AfiI CCNNNNNNNGG 3 cut(s) 189, 419, 486
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 5 cut(s) 390, 672, 677, 794, 841
AjnI CCWGG 2 cut(s) 446, 459
AleI CACNNNNGTG 1 cut(s) 920
AluBI AGCT 7 cut(s) 94, 172, 198, 359, 517, 892, 954
AluI AGCT 7 cut(s) 94, 172, 198, 359, 517, 892, 954
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 3 cut(s) 12, 287, 562
Aor13HI TCCGGA 1 cut(s) 295
ApeKI GCWGC 3 cut(s) 252, 509, 521
AspS9I GGNCC 2 cut(s) 422, 587
AsuHPI GGTGA 3 cut(s) 577, 767, 907
AsuNHI GCTAGC 1 cut(s) 517
AvaII GGWCC 2 cut(s) 422, 587
BaeGI GKGCMC 1 cut(s) 972
BanI GGYRCC 2 cut(s) 395, 969
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 3 cut(s) 239, 521, 533
BccI CCATC 3 cut(s) 251, 614, 842
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 2 cut(s) 448, 461
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 4 cut(s) 30, 518, 615, 858
BfmI CTRYAG 1 cut(s) 507
BisI GCNGC 5 cut(s) 253, 348, 510, 522, 760
BlpI GCTNAGC 2 cut(s) 194, 649
BlsI GCNGC 5 cut(s) 254, 349, 511, 523, 761
BmcAI AGTACT 2 cut(s) 638, 809
Bme1390I CCNGG 2 cut(s) 448, 461
Bme18I GGWCC 2 cut(s) 422, 587
BmgT120I GGNCC 2 cut(s) 422, 587
BmiI GGNNCC 4 cut(s) 181, 397, 424, 971
BmrFI CCNGG 2 cut(s) 448, 461
BmrI ACTGGG 3 cut(s) 66, 566, 914
BmsI GCATC 2 cut(s) 229, 284
BmtI GCTAGC 1 cut(s) 521
BmuI ACTGGG 3 cut(s) 66, 566, 914
BpiI GAAGAC 1 cut(s) 370
BpmI CTGGAG 2 cut(s) 84, 482
Bpu10I CCTNAGC 1 cut(s) 360
Bpu1102I GCTNAGC 2 cut(s) 194, 649
BpuEI CTTGAG 2 cut(s) 220, 617
BsaJI CCNNGG 1 cut(s) 778
BsaWI WCCGGW 2 cut(s) 295, 743
BsaXI ACNNNNNCTCC 4 cut(s) 579, 609, 774, 804
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 419, 486
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 5 cut(s) 61, 491, 572, 649, 920
Bse3DI GCAATG 2 cut(s) 72, 243
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 2 cut(s) 448, 461
BseDI CCNNGG 1 cut(s) 778
BseGI GGATG 3 cut(s) 275, 535, 703
BseLI CCNNNNNNNGG 3 cut(s) 189, 419, 486
BseMI GCAATG 2 cut(s) 72, 243
BseMII CTCAG 2 cut(s) 36, 663
BseNI ACTGG 5 cut(s) 61, 491, 572, 649, 920
BseSI GKGCMC 1 cut(s) 972
BseXI GCAGC 3 cut(s) 239, 521, 533
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 748
BshNI GGYRCC 2 cut(s) 395, 969
BsiEI CGRYCG 1 cut(s) 748
BsiSI CCGG 3 cut(s) 296, 314, 744
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 3 cut(s) 189, 419, 486
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 972
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 6 cut(s) 10, 17, 211, 292, 339, 567
Bsp1720I GCTNAGC 2 cut(s) 194, 649
BspACI CCGC 4 cut(s) 348, 590, 759, 1002
BspCNI CTCAG 2 cut(s) 35, 662
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 4 cut(s) 181, 397, 424, 971
BspMAI CTGCAG 1 cut(s) 511
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 3 cut(s) 12, 287, 562
BspT107I GGYRCC 2 cut(s) 395, 969
BsrDI GCAATG 2 cut(s) 72, 243
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 5 cut(s) 61, 491, 572, 649, 920
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 778
BssMI GATC 6 cut(s) 10, 17, 211, 292, 339, 567
Bst2UI CCWGG 2 cut(s) 448, 461
Bst4CI ACNGT 5 cut(s) 46, 622, 769, 779, 946
BstC8I GCNNGC 3 cut(s) 250, 519, 956
BstDEI CTNAG 4 cut(s) 22, 194, 360, 649
BstDSI CCRYGG 1 cut(s) 778
BstF5I GGATG 3 cut(s) 275, 535, 703
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 6 cut(s) 13, 20, 214, 295, 342, 570
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 6 cut(s) 10, 17, 211, 292, 339, 567
BstMCI CGRYCG 1 cut(s) 748
BstMWI GCNNNNNNNGC 4 cut(s) 258, 356, 518, 589
BstNI CCWGG 2 cut(s) 448, 461
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 2 cut(s) 446, 459
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 972
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 3 cut(s) 239, 521, 533
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BtgI CCRYGG 1 cut(s) 778
BtgZI GCGATG 1 cut(s) 270
BtsCI GGATG 3 cut(s) 275, 535, 703
BtsIMutI CAGTG 3 cut(s) 618, 927, 942
Cac8I GCNNGC 3 cut(s) 250, 519, 956
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 422, 587
Csp6I GTAC 3 cut(s) 490, 637, 808
CviAII CATG 3 cut(s) 116, 406, 689
CviQI GTAC 3 cut(s) 490, 637, 808
DdeI CTNAG 4 cut(s) 22, 194, 360, 649
DpnI GATC 6 cut(s) 12, 19, 213, 294, 341, 569
DpnII GATC 6 cut(s) 10, 17, 211, 292, 339, 567
EciI GGCGGA 1 cut(s) 991
Eco47I GGWCC 2 cut(s) 422, 587
Eco57I CTGAAG 1 cut(s) 375
EcoO109I RGGNCCY 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 446, 459
FaeI CATG 3 cut(s) 119, 409, 692
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 408
FatI CATG 3 cut(s) 115, 405, 688
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 5 cut(s) 253, 348, 510, 522, 760
FokI GGATG 3 cut(s) 262, 542, 710
Fsp4HI GCNGC 5 cut(s) 253, 348, 510, 522, 760
FspBI CTAG 4 cut(s) 30, 518, 615, 858
GluI GCNGC 5 cut(s) 253, 348, 510, 522, 760
GsuI CTGGAG 2 cut(s) 84, 482
HapII CCGG 3 cut(s) 296, 314, 744
Hin1II CATG 3 cut(s) 119, 409, 692
HindIII AAGCTT 2 cut(s) 196, 890
HinfI GANTC 1 cut(s) 443
HpaII CCGG 3 cut(s) 296, 314, 744
HphI GGTGA 3 cut(s) 577, 767, 907
Hpy166II GTNNAC 3 cut(s) 403, 553, 915
Hpy188I TCNGA 2 cut(s) 15, 355
Hpy188III TCNNGA 5 cut(s) 101, 218, 296, 425, 949
Hpy8I GTNNAC 3 cut(s) 403, 553, 915
HpyAV CCTTC 3 cut(s) 107, 170, 782
HpyCH4III ACNGT 5 cut(s) 46, 622, 769, 779, 946
HpyCH4V TGCA 4 cut(s) 471, 509, 688, 958
HpyF10VI GCNNNNNNNGC 4 cut(s) 258, 356, 518, 589
HpyF3I CTNAG 4 cut(s) 22, 194, 360, 649
Hsp92II CATG 3 cut(s) 119, 409, 692
Kpn2I TCCGGA 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 6 cut(s) 10, 17, 211, 292, 339, 567
LmnI GCTCC 3 cut(s) 99, 179, 925
Lsp1109I GCAGC 3 cut(s) 239, 521, 533
LweI GCATC 2 cut(s) 229, 284
MaeI CTAG 4 cut(s) 30, 518, 615, 858
MaeIII GTNAC 3 cut(s) 52, 309, 455
MalI GATC 6 cut(s) 12, 19, 213, 294, 341, 569
MboI GATC 6 cut(s) 10, 17, 211, 292, 339, 567
MboII GAAGA 5 cut(s) 206, 311, 370, 378, 857
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 972
MluCI AATT 3 cut(s) 667, 672, 1007
MluI ACGCGT 1 cut(s) 370
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 622
MroI TCCGGA 1 cut(s) 295
MseI TTAA 5 cut(s) 234, 366, 693, 894, 975
MslI CAYNNNNRTG 2 cut(s) 719, 920
MspI CCGG 3 cut(s) 296, 314, 744
MspR9I CCNGG 2 cut(s) 448, 461
MvaI CCWGG 2 cut(s) 448, 461
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 4 cut(s) 258, 356, 518, 589
NdeII GATC 6 cut(s) 10, 17, 211, 292, 339, 567
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 3 cut(s) 119, 409, 692
NlaIV GGNNCC 4 cut(s) 181, 397, 424, 971
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 1 cut(s) 692
OliI CACNNNNGTG 1 cut(s) 920
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PflFI GACNNNGTC 1 cut(s) 944
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 5 cut(s) 254, 349, 511, 523, 761
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PpuMI RGGWCCY 1 cut(s) 422
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 2 cut(s) 446, 459
PspGI CCWGG 2 cut(s) 446, 459
PspN4I GGNNCC 4 cut(s) 181, 397, 424, 971
PspPI GGNCC 2 cut(s) 422, 587
PspPPI RGGWCCY 1 cut(s) 422
PstI CTGCAG 1 cut(s) 511
PsuI RGATCY 1 cut(s) 17
PsyI GACNNNGTC 1 cut(s) 944
RsaI GTAC 3 cut(s) 491, 638, 809
RsaNI GTAC 3 cut(s) 490, 637, 808
RseI CAYNNNNRTG 2 cut(s) 719, 920
SaqAI TTAA 5 cut(s) 234, 366, 693, 894, 975
SatI GCNGC 5 cut(s) 253, 348, 510, 522, 760
Sau3AI GATC 6 cut(s) 10, 17, 211, 292, 339, 567
Sau96I GGNCC 2 cut(s) 422, 587
ScaI AGTACT 2 cut(s) 638, 809
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 448, 461
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 2 cut(s) 229, 284
SfcI CTRYAG 1 cut(s) 507
SinI GGWCC 2 cut(s) 422, 587
SmiMI CAYNNNNRTG 2 cut(s) 719, 920
SmlI CTYRAG 2 cut(s) 199, 632
SmoI CTYRAG 2 cut(s) 199, 632
Sse9I AATT 3 cut(s) 667, 672, 1007
SsiI CCGC 4 cut(s) 348, 590, 759, 1002
SspMI CTAG 4 cut(s) 30, 518, 615, 858
StyD4I CCNGG 2 cut(s) 446, 459
TaaI ACNGT 5 cut(s) 46, 622, 769, 779, 946
TaqI TCGA 3 cut(s) 210, 219, 438
TasI AATT 3 cut(s) 667, 672, 1007
TatI WGTACW 2 cut(s) 636, 807
TauI GCSGC 2 cut(s) 350, 762
Tru1I TTAA 5 cut(s) 234, 366, 693, 894, 975
Tru9I TTAA 5 cut(s) 234, 366, 693, 894, 975
TscAI CASTG 3 cut(s) 625, 927, 949
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 3 cut(s) 252, 509, 521
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 5 cut(s) 61, 149, 422, 546, 741
TspRI CASTG 3 cut(s) 625, 927, 949
Tth111I GACNNNGTC 1 cut(s) 944
Van91I CCANNNNNTGG 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 422, 587
XceI RCATGY 1 cut(s) 692
XspI CTAG 4 cut(s) 30, 518, 615, 858
ZrmI AGTACT 2 cut(s) 638, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.