RLG00000029212

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002169
Physical Location & Seq
Reverse (-)
34594894 .. 34596499
1606 bp
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UTR
Exon/CDS
Intron
RLM00000029212

Sequence Viewer

Length: 705 bp
ATGAACCCCAACCCCAACAGAGGTCCTGATGTGGTTGTCGATGATTGGAGTGACTTGGAGTTTTGCAAGAACACCAAGAACTGGTACTGCTATAGGAAAACTGTGGCAGAGCAAGTAGCGTGGGATGAAGCTAAAGAGAGAGGGGTGGACTTGGTTGTGGTGAACCCAGTTTTGGTGGTTGGACCACTGCTCCAACCAACCACCAACGCTAGCACTGTCCACATCCTCAAGTACTTGACTGGCTCAGCTAAGACTTATGCCAATTCAATTCAGACCTATGTGCATGTTAAGGATGTGGCATTAGCACACATACTAGTGTATGAAACTCCCTCTGCCTCCGGTCGTTATCTTTGCGGCGAGAGCATCCTTCACCGTGGAGATGTGGTTGAAATCCTTGCCAAGTTCTTCCCTGAATACCCAATACCCACCAAGTTGAAAGTTGGTGGGAAACCCAGAGCAAAACCATACAAGTTCTCAAACCAGAAGCTACAAGACTTGGGTTTGGAGTTCACCCCCGTGAAACAGAGCCTATATGACACTGTCAAGAGCTTGCAGGACAAGGGGCACCTTAAAGTTCCTACAAAACAAGAAGAAGACTCCATTAAGATCCAATACCTGAGTATTTATTTTCTAGCTCTTGCACACCTTATCGAAATACCAAATCTCGTGCCTTGGTTCATTGCTTTTCTATATGTACTATCCTAA

Protein Analysis

235

Amino Acids

26.45

Weight (kDa)

8.27

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 21 - 113 1.1e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 564
AccB7I CCANNNNNTGG 1 cut(s) 81
AciI CCGC 1 cut(s) 354
AclWI GGATC 1 cut(s) 601
AfaI GTAC 3 cut(s) 86, 233, 696
AfiI CCNNNNNNNGG 3 cut(s) 20, 81, 172
AgsI TTSAA 3 cut(s) 267, 389, 436
AhlI ACTAGT 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AleI CACNNNNGTG 1 cut(s) 515
AluBI AGCT 5 cut(s) 131, 248, 487, 549, 635
AluI AGCT 5 cut(s) 131, 248, 487, 549, 635
AlwI GGATC 1 cut(s) 601
AspS9I GGNCC 2 cut(s) 23, 182
AsuHPI GGTGA 3 cut(s) 172, 362, 502
AsuNHI GCTAGC 1 cut(s) 209
AvaII GGWCC 2 cut(s) 23, 182
BaeGI GKGCMC 1 cut(s) 567
BanI GGYRCC 1 cut(s) 564
BauI CACGAG 1 cut(s) 665
BbsI GAAGAC 1 cut(s) 600
BcuI ACTAGT 1 cut(s) 313
BfaI CTAG 3 cut(s) 210, 314, 632
BfmI CTRYAG 1 cut(s) 91
BisI GCNGC 1 cut(s) 355
BlpI GCTNAGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 356
BmcAI AGTACT 1 cut(s) 233
Bme18I GGWCC 2 cut(s) 23, 182
BmgT120I GGNCC 2 cut(s) 23, 182
BmiI GGNNCC 1 cut(s) 566
BmrI ACTGGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 372
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 600
Bpu1102I GCTNAGC 1 cut(s) 244
BpuEI CTTGAG 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 373, 671
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 369, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 81, 172
Bse1I ACTGG 3 cut(s) 86, 167, 244
Bse3DI GCAATG 1 cut(s) 678
BseDI CCNNGG 2 cut(s) 373, 671
BseGI GGATG 4 cut(s) 130, 222, 298, 363
BseLI CCNNNNNNNGG 3 cut(s) 20, 81, 172
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 258, 608
BseNI ACTGG 3 cut(s) 86, 167, 244
BseSI GKGCMC 1 cut(s) 567
Bsh1285I CGRYCG 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 564
BsiEI CGRYCG 1 cut(s) 343
BsiSI CCGG 1 cut(s) 339
BslI CCNNNNNNNGG 3 cut(s) 20, 81, 172
Bsp1286I GDGCHC 1 cut(s) 567
Bsp143I GATC 1 cut(s) 606
Bsp1720I GCTNAGC 1 cut(s) 244
BspACI CCGC 1 cut(s) 354
BspCNI CTCAG 2 cut(s) 257, 609
BspLI GGNNCC 1 cut(s) 566
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 564
BsrDI GCAATG 1 cut(s) 678
BsrI ACTGG 3 cut(s) 86, 167, 244
BssECI CCNNGG 2 cut(s) 373, 671
BssMI GATC 1 cut(s) 606
BssSI CACGAG 1 cut(s) 665
BssT1I CCWWGG 1 cut(s) 671
Bst2BI CACGAG 1 cut(s) 665
Bst4CI ACNGT 4 cut(s) 103, 217, 374, 541
BstC8I GCNNGC 2 cut(s) 211, 551
BstDEI CTNAG 3 cut(s) 244, 249, 617
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 4 cut(s) 130, 222, 298, 363
BstKTI GATC 1 cut(s) 609
BstMBI GATC 1 cut(s) 606
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 360
BstNSI RCATGY 1 cut(s) 287
BstSFI CTRYAG 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 567
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 1 cut(s) 606
BstYI RGATCY 1 cut(s) 606
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 4 cut(s) 130, 222, 298, 363
BtsI GCAGTG 1 cut(s) 185
BtsIMutI CAGTG 3 cut(s) 185, 213, 537
Cac8I GCNNGC 2 cut(s) 211, 551
Cfr13I GGNCC 2 cut(s) 23, 182
Csp6I GTAC 3 cut(s) 85, 232, 695
CspCI CAANNNNNGTGG 2 cut(s) 101, 136
CviAII CATG 1 cut(s) 284
CviJI RGCY 7 cut(s) 131, 243, 248, 487, 528, 549, 635
CviKI_1 RGCY 7 cut(s) 131, 243, 248, 487, 528, 549, 635
CviQI GTAC 3 cut(s) 85, 232, 695
DdeI CTNAG 3 cut(s) 244, 249, 617
DpnI GATC 1 cut(s) 608
DpnII GATC 1 cut(s) 606
Eco130I CCWWGG 1 cut(s) 671
Eco47I GGWCC 2 cut(s) 23, 182
EcoO109I RGGNCCY 1 cut(s) 23
EcoT14I CCWWGG 1 cut(s) 671
ErhI CCWWGG 1 cut(s) 671
FaeI CATG 1 cut(s) 287
FatI CATG 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 355
FokI GGATG 4 cut(s) 137, 209, 305, 350
Fsp4HI GCNGC 1 cut(s) 355
FspBI CTAG 3 cut(s) 210, 314, 632
GluI GCNGC 1 cut(s) 355
HapII CCGG 1 cut(s) 339
Hin1II CATG 1 cut(s) 287
HinfI GANTC 1 cut(s) 596
HpaII CCGG 1 cut(s) 339
HphI GGTGA 3 cut(s) 172, 362, 502
Hpy166II GTNNAC 4 cut(s) 148, 163, 220, 510
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 2 cut(s) 26, 544
Hpy8I GTNNAC 4 cut(s) 148, 163, 220, 510
HpyAV CCTTC 1 cut(s) 377
HpyCH4III ACNGT 4 cut(s) 103, 217, 374, 541
HpyCH4V TGCA 4 cut(s) 66, 283, 553, 641
HpyF10VI GCNNNNNNNGC 1 cut(s) 360
HpyF3I CTNAG 3 cut(s) 244, 249, 617
Hsp92II CATG 1 cut(s) 287
Kzo9I GATC 1 cut(s) 606
LmnI GCTCC 1 cut(s) 195
LweI GCATC 1 cut(s) 372
MaeI CTAG 3 cut(s) 210, 314, 632
MaeIII GTNAC 1 cut(s) 50
MalI GATC 1 cut(s) 608
MboI GATC 1 cut(s) 606
MboII GAAGA 3 cut(s) 397, 602, 605
MflI RGATCY 1 cut(s) 606
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 2 cut(s) 262, 267
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 2 cut(s) 160, 217
MnlI CCTC 5 cut(s) 14, 134, 236, 340, 346
MseI TTAA 3 cut(s) 288, 570, 603
MslI CAYNNNNRTG 2 cut(s) 314, 515
MspI CCGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 360
NdeII GATC 1 cut(s) 606
NheI GCTAGC 1 cut(s) 209
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 50
NspI RCATGY 1 cut(s) 287
OliI CACNNNNGTG 1 cut(s) 515
PflFI GACNNNGTC 1 cut(s) 539
PflMI CCANNNNNTGG 1 cut(s) 81
PkrI GCNGC 1 cut(s) 356
PleI GAGTC 1 cut(s) 590
PpsI GAGTC 1 cut(s) 590
PpuMI RGGWCCY 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 23
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 2 cut(s) 23, 182
PspPPI RGGWCCY 1 cut(s) 23
PsuI RGATCY 1 cut(s) 606
PsyI GACNNNGTC 1 cut(s) 539
RsaI GTAC 3 cut(s) 86, 233, 696
RsaNI GTAC 3 cut(s) 85, 232, 695
RseI CAYNNNNRTG 2 cut(s) 314, 515
SaqAI TTAA 3 cut(s) 288, 570, 603
SatI GCNGC 1 cut(s) 355
Sau3AI GATC 1 cut(s) 606
Sau96I GGNCC 2 cut(s) 23, 182
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 1 cut(s) 590
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 1 cut(s) 372
SfcI CTRYAG 1 cut(s) 91
SinI GGWCC 2 cut(s) 23, 182
SmiMI CAYNNNNRTG 2 cut(s) 314, 515
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SpeI ACTAGT 1 cut(s) 313
Sse9I AATT 2 cut(s) 262, 267
SsiI CCGC 1 cut(s) 354
SspMI CTAG 3 cut(s) 210, 314, 632
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 4 cut(s) 103, 217, 374, 541
TaqI TCGA 2 cut(s) 39, 651
TasI AATT 2 cut(s) 262, 267
TatI WGTACW 2 cut(s) 231, 694
TauI GCSGC 1 cut(s) 357
Tru1I TTAA 3 cut(s) 288, 570, 603
Tru9I TTAA 3 cut(s) 288, 570, 603
TscAI CASTG 3 cut(s) 192, 220, 544
TseFI GTSAC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 4 cut(s) 17, 141, 336, 667
TspRI CASTG 3 cut(s) 192, 220, 544
Tth111I GACNNNGTC 1 cut(s) 539
Van91I CCANNNNNTGG 1 cut(s) 81
VpaK11BI GGWCC 2 cut(s) 23, 182
XceI RCATGY 1 cut(s) 287
XspI CTAG 3 cut(s) 210, 314, 632
ZrmI AGTACT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.