Rh2BG367700

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002169
Physical Location & Seq
Reverse (-)
51303041 .. 51305387
2347 bp
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UTR
Exon/CDS
Intron
Rh2BG367700.1

Sequence Viewer

Length: 618 bp
ATGGACCCGACGAGAGGTCCCGATGTGGTTGTTGATGAGTCATGTTGGAGTGATCTGGAGTTTTGCAAGAACACCAAGAACTGGTACTGCTATGGGAAAGCTGTGGCGGAGCAAGCAGCGTGGGATGAGGCCAAAGAGAAAGGAGTAGACTTGGTGGTGGTGAACCCAGTTTTGGTGCTTGGACCACTGCTCCAACCAACCATCAACGCCAGCATTATCCACGTCCTCAAGTACTTGACTGGCTCGGCCAAGACTTATGCCAATTCTGTTCAGGCCTATGTGCATGTTAAGGACGTGGCATTAGCACACATACTGGTGTATGAAACTCCCTCGGCATCTGGCCGTTATCTTTGCGCCGAGAGCGTCCTTCACCGTGGAGATGTAGTTGAAATCCTCGCCAAGTTCTTCCCTGAATACCCGATACCCACCAAGTTGAAAGATGATGGGAAACCCAGAGCAATACCCTACAAGTTCTCAAATCAGAAGCTACAAGACTTGGGTTTGGAGTTCACTCCAGTGAAACAGAGCCTATATGATACTGTCAAGAGCTTGCAGGAGAAGGGTCACCTTAAAGTTCCTACAAAACAAGAACAAGACTCCATTAAGATCCAATCTTAA

Protein Analysis

205

Amino Acids

22.88

Weight (kDa)

6.52

Isoelectric Point (pI)

33.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 21 - 113 3.1e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 81
AccI GTMKAC 1 cut(s) 147
AciI CCGC 1 cut(s) 107
AclWI GGATC 1 cut(s) 601
AcoI YGGCCR 2 cut(s) 246, 340
AfaI GTAC 2 cut(s) 86, 233
AfiI CCNNNNNNNGG 3 cut(s) 14, 81, 172
AgsI TTSAA 2 cut(s) 389, 436
AhdI GACNNNNNGTC 1 cut(s) 15
AjiI CACGTC 2 cut(s) 223, 295
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AleI CACNNNNGTG 1 cut(s) 515
AluBI AGCT 3 cut(s) 101, 487, 549
AluI AGCT 3 cut(s) 101, 487, 549
AlwI GGATC 1 cut(s) 601
AoxI GGCC 4 cut(s) 129, 246, 273, 340
ApeKI GCWGC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 3 cut(s) 4, 17, 182
AsuHPI GGTGA 3 cut(s) 172, 362, 557
AvaII GGWCC 3 cut(s) 4, 17, 182
BbvI GCAGC 1 cut(s) 128
BccI CCATC 2 cut(s) 209, 437
BceAI ACGGC 1 cut(s) 327
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
BmcAI AGTACT 1 cut(s) 233
Bme18I GGWCC 3 cut(s) 4, 17, 182
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgBI CACGTC 2 cut(s) 223, 295
BmgT120I GGNCC 3 cut(s) 4, 17, 182
BmiI GGNNCC 2 cut(s) 6, 19
BmrI ACTGGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 344
BmuI ACTGGG 1 cut(s) 161
BpmI CTGGAG 2 cut(s) 77, 498
BpuEI CTTGAG 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 330, 373
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 369, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 81, 172
Bse1I ACTGG 5 cut(s) 86, 167, 244, 318, 515
BseDI CCNNGG 2 cut(s) 330, 373
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 3 cut(s) 14, 81, 172
BseNI ACTGG 5 cut(s) 86, 167, 244, 318, 515
BseXI GCAGC 1 cut(s) 128
BshFI GGCC 4 cut(s) 131, 248, 275, 342
BslFI GGGAC 1 cut(s) 3
BslI CCNNNNNNNGG 3 cut(s) 14, 81, 172
BsmFI GGGAC 1 cut(s) 3
BsnI GGCC 4 cut(s) 131, 248, 275, 342
Bsp143I GATC 2 cut(s) 52, 606
BspACI CCGC 1 cut(s) 107
BspANI GGCC 4 cut(s) 131, 248, 275, 342
BspLI GGNNCC 2 cut(s) 6, 19
BspPI GGATC 1 cut(s) 601
BsrI ACTGG 5 cut(s) 86, 167, 244, 318, 515
BssECI CCNNGG 2 cut(s) 330, 373
BssMI GATC 2 cut(s) 52, 606
Bst4CI ACNGT 2 cut(s) 374, 541
BstC8I GCNNGC 3 cut(s) 114, 211, 551
BstDSI CCRYGG 1 cut(s) 373
BstEII GGTNACC 1 cut(s) 563
BstF5I GGATG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 2 cut(s) 55, 609
BstMBI GATC 2 cut(s) 52, 606
BstMWI GCNNNNNNNGC 2 cut(s) 113, 360
BstNSI RCATGY 1 cut(s) 287
BstPI GGTNACC 1 cut(s) 563
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 606
BstYI RGATCY 1 cut(s) 606
BsuRI GGCC 4 cut(s) 131, 248, 275, 342
BtgI CCRYGG 1 cut(s) 373
BtrI CACGTC 2 cut(s) 223, 295
BtsCI GGATG 1 cut(s) 130
BtsI GCAGTG 1 cut(s) 185
BtsIMutI CAGTG 2 cut(s) 185, 522
Cac8I GCNNGC 3 cut(s) 114, 211, 551
CfoI GCGC 1 cut(s) 356
Cfr13I GGNCC 3 cut(s) 4, 17, 182
CseI GACGC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 85, 232
CspCI CAANNNNNGTGG 2 cut(s) 101, 136
CviAII CATG 2 cut(s) 42, 284
CviJI RGCY 9 cut(s) 101, 131, 243, 248, 275, 342, 487, 528, 549
CviKI_1 RGCY 9 cut(s) 101, 131, 243, 248, 275, 342, 487, 528, 549
CviQI GTAC 2 cut(s) 85, 232
DpnI GATC 2 cut(s) 54, 608
DpnII GATC 2 cut(s) 52, 606
DriI GACNNNNNGTC 1 cut(s) 15
EaeI YGGCCR 2 cut(s) 246, 340
Eam1105I GACNNNNNGTC 1 cut(s) 15
EciI GGCGGA 1 cut(s) 122
Eco147I AGGCCT 1 cut(s) 275
Eco47I GGWCC 3 cut(s) 4, 17, 182
Eco91I GGTNACC 1 cut(s) 563
EcoO109I RGGNCCY 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 563
FaeI CATG 2 cut(s) 45, 287
FaiI YATR 9 cut(s) 43, 93, 258, 279, 285, 311, 321, 532, 534
FaqI GGGAC 1 cut(s) 3
FatI CATG 2 cut(s) 41, 283
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 1 cut(s) 117
GlaI GCGC 1 cut(s) 355
GluI GCNGC 1 cut(s) 117
GsuI CTGGAG 2 cut(s) 77, 498
HaeIII GGCC 4 cut(s) 131, 248, 275, 342
HgaI GACGC 1 cut(s) 352
HhaI GCGC 1 cut(s) 356
Hin1II CATG 2 cut(s) 45, 287
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 2 cut(s) 38, 596
HphI GGTGA 3 cut(s) 172, 362, 557
Hpy166II GTNNAC 3 cut(s) 148, 163, 510
Hpy188I TCNGA 1 cut(s) 483
Hpy188III TCNNGA 3 cut(s) 20, 56, 544
Hpy8I GTNNAC 3 cut(s) 148, 163, 510
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 377, 553
HpyCH4III ACNGT 2 cut(s) 374, 541
HpyCH4IV ACGT 2 cut(s) 222, 294
HpyCH4V TGCA 3 cut(s) 66, 283, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 360
HpySE526I ACGT 2 cut(s) 222, 294
Hsp92II CATG 2 cut(s) 45, 287
HspAI GCGC 1 cut(s) 354
Kzo9I GATC 2 cut(s) 52, 606
LmnI GCTCC 2 cut(s) 109, 195
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 344
MaeII ACGT 2 cut(s) 222, 294
MaeIII GTNAC 1 cut(s) 563
MalI GATC 2 cut(s) 54, 608
MboI GATC 2 cut(s) 52, 606
MboII GAAGA 1 cut(s) 397
MflI RGATCY 1 cut(s) 606
MluCI AATT 1 cut(s) 262
MlyI GAGTC 2 cut(s) 47, 590
MmeI TCCRAC 2 cut(s) 26, 217
MnlI CCTC 5 cut(s) 8, 121, 236, 340, 404
MseI TTAA 4 cut(s) 288, 570, 603, 616
MslI CAYNNNNRTG 2 cut(s) 314, 515
MwoI GCNNNNNNNGC 2 cut(s) 113, 360
NdeII GATC 2 cut(s) 52, 606
NlaIII CATG 2 cut(s) 45, 287
NlaIV GGNNCC 2 cut(s) 6, 19
NmeAIII GCCGAG 3 cut(s) 224, 311, 382
NmuCI GTSAC 1 cut(s) 563
NspI RCATGY 1 cut(s) 287
OliI CACNNNNGTG 1 cut(s) 515
PceI AGGCCT 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 81
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 46, 590
PpsI GAGTC 2 cut(s) 46, 590
PpuMI RGGWCCY 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 17
PspEI GGTNACC 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 6, 19
PspPI GGNCC 3 cut(s) 4, 17, 182
PspPPI RGGWCCY 1 cut(s) 17
PsuI RGATCY 1 cut(s) 606
RsaI GTAC 2 cut(s) 86, 233
RsaNI GTAC 2 cut(s) 85, 232
RseI CAYNNNNRTG 2 cut(s) 314, 515
SaqAI TTAA 4 cut(s) 288, 570, 603, 616
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 2 cut(s) 52, 606
Sau96I GGNCC 3 cut(s) 4, 17, 182
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 2 cut(s) 47, 590
SetI ASST 7 cut(s) 19, 103, 225, 297, 489, 551, 570
SfaNI GCATC 1 cut(s) 344
SinI GGWCC 3 cut(s) 4, 17, 182
SmiMI CAYNNNNRTG 2 cut(s) 314, 515
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 1 cut(s) 262
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 1 cut(s) 107
StuI AGGCCT 1 cut(s) 275
TaaI ACNGT 2 cut(s) 374, 541
TaiI ACGT 2 cut(s) 225, 297
TasI AATT 1 cut(s) 262
TatI WGTACW 1 cut(s) 231
Tru1I TTAA 4 cut(s) 288, 570, 603, 616
Tru9I TTAA 4 cut(s) 288, 570, 603, 616
TscAI CASTG 2 cut(s) 192, 522
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 563
TspDTI ATGAA 1 cut(s) 336
TspRI CASTG 2 cut(s) 192, 522
Van91I CCANNNNNTGG 1 cut(s) 81
VpaK11BI GGWCC 3 cut(s) 4, 17, 182
XceI RCATGY 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 147
ZrmI AGTACT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.