Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001908

Physical Location & Seq

Genomic Coordinates Reverse (-)

4598854 .. 4604465

Gene Structure 5612 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1035600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1416 bp

ATGGCTAAAGCACAAAGCTTCTACGCTGTGTGCTTAGCATTTTCTTTAGTGTTTAATCTATTGCTGAGCTTCAGACTCTATGTGGATGTGGGTGGAGATTGGGAGCTGAGTTGGAGCAAAAGAGCAGCAGAAGAAGCTGAGCACGTGGCAACCATCTCTTGCTCAGGGCATGGAAGAGCCTACTTAGATGGCCTAATTCTTGATGGGAAGGAACCTGTTTGTGAGTGCAATTCCTGCTATGGAGGCCATGACTGCTCTTTGTTTTTAACTGGATGTGCAGCAAACGCCGACAGTGGGGACCCGTATTTCTTGGAGCCTTTTTGGACGAAACATGCATCAAAAAGTGCAGTTTTAGTAGCAGGATGGCATCGAATGAGCTATGTCTTCCCTGATCAATCTCGCATCTCAGCAGAGCTTGAGAGGCACATCCGCGAAATTCATACCATTGTCGGAAATGCGGTCACTGAAGGGAGATACATTGTTTTTGGTGCTGGCTCAACCCAACTTCTCAATGCTGCAGTTAACGCACTGTCGACTTCCAATAACTTCAACTCTTCACCTTCAAGTGTTGTAGCTTCAATTCCTTATTACAGTCTTTACCAAATGCAAACGGAGTTCTTCCGATCAATGGACTACATGTTTCAGGGAGATGCTGCCTTGCTGCAGAACACTTCGGATGCTACTAAAAATGTTGTTGAGTTTGTAACCTCACCAAACAATCCAGATGGGCAGCTGAAGAAGGCAATTTTTGAAGGCCCGAATGCTAAAGCAATCTATGATCGTGTATACTACTGGCCACATTTTACAGCAATTCCTGCTCCAGCAGATGACGATCTCATGATATTTACAATTTCTAAGCTCACTGGCCATGCCGGTACTAGATTCGGGTGGGCATTGGTAAAGGATGAGTCTGTGTACCAAAGGATGACCCAGCATATACTCATGAGTAGCTTAGGTATTTCTCGAGATGCTCAGCTAAGAGCTTTGAAGCTTCTAAATGTCGTCCTTGAAACCGGAGGCAGGGAAATCTTTGAGTTCGGATACCACACTTTGAAGAAACGCTGGGAAAATTTGACAAACATCCTGTCTCTGTCAAACCGCTTTACTCTTCAAAAGTTTGAACCCAAATACTGCAACTTTTTCAAGAATATTAGAGACCCTTCACCAGCTTATGCATGGGTGAAGTGCGAGAGGGAAGAGGATAAAGATTGCTATGAAGTTCTGAAACAAGAAGCGAATGTTTATGGTCGTCCAGGTGCTGAATTTGGAGCTGAAGATCGATATGTTCGTCTCACCCTCTTAAGGAACCAAGATGATTTTGATTTATTGCTTCAGCGACTCAACCAATTAGTCTTGGATGAAGGGAAGAGCAAAATAATTATTTCTTCTCAAGTAGGGGGTCAATACGTGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alliinase_C EGF_alliinase

Protein Analysis

472

Amino Acids

52.99

Weight (kDa)

5.82

Isoelectric Point (pI)

39.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EGF_alliinase	PF04863	37 - 92	1.7e-28	Alliinase EGF-like domain
Alliinase_C	PF04864	94 - 451	1.5e-151	Allinase
Aminotran_1_2	PF00155	134 - 447	2.1e-08	Aminotransferase class I and II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34040 AT1G34040 AT1G34060 AT1G34060
fragaria_vesca	FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010
malus_domestica	MD05G1033800.v1.1 MD10G1035100.v1.1 MD10G1035600.v1.1 MD10G1035700.v1.1 MD10G1035800.v1.1
prunus_persica	Prupe.8G041100_v2.0.a1 Prupe.8G041200_v2.0.a1 Prupe.8G041300_v2.0.a1 Prupe.8G041600_v2.0.a1 Prupe.8G041600_v2.0.a1
pyrus_communis	pycom05g02440 pycom10g02410 pycom10g02420
rosa_chinensis	RchiOBHm_Chr6g0274171 RchiOBHm_Chr6g0274191
rosa_laevigata	RLG00000013573 RLG00000013574 RLG00000013575
rosa_multiflora	Rmu_co8325345.1_g000001 Rmu_co8467135.1_g000001 Rmu_sc0001939.1_g000013 Rmu_sc0001939.1_g000014 Rmu_sc0001939.1_g000015
rosa_roxburghii	Rroxscaffold_7G00194440 Rroxscaffold_7G00194490
rosa_rugosa	Rorug06G0079700
rosa_samantha	Rh6AG194800 Rh6AG194900 Rh6BG199000 Rh6BG199200 Rh6CG199400 Rh6DG190700 Rh6DG190900
rosa_wichuraiana	Rw6G017030 Rw6G017040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	533, 786
AccII	CGCG	1 cut(s)	432
AciI	CCGC	3 cut(s)	430, 458, 1099
AcoI	YGGCCR	2 cut(s)	794, 865
AcsI	RAATTY	3 cut(s)	435, 1069, 1262
AcuI	CTGAAG	5 cut(s)	55, 486, 755, 1293, 1316
AcvI	CACGTG	1 cut(s)	145
AfaI	GTAC	2 cut(s)	877, 917
AfiI	CCNNNNNNNGG	4 cut(s)	294, 628, 1020, 1302
AflII	CTTAAG	1 cut(s)	1300
AflIII	ACRYGT	1 cut(s)	636
AjnI	CCWGG	1 cut(s)	1252
Alw21I	GWGCWC	1 cut(s)	144
Alw26I	GTCTC	3 cut(s)	1092, 1149, 1295
AlwNI	CAGNNNCTG	1 cut(s)	1259
Ama87I	CYCGRG	1 cut(s)	963
AoxI	GGCC	5 cut(s)	190, 244, 754, 794, 865
ApeKI	GCWGC	6 cut(s)	125, 278, 515, 653, 661, 730
ApoI	RAATTY	3 cut(s)	435, 1069, 1262
ArsI	GACNNNNNNTTYG	2 cut(s)	1070, 1102
AspS9I	GGNCC	2 cut(s)	298, 755
AsuHPI	GGTGA	5 cut(s)	549, 702, 1155, 1192, 1285
AvaI	CYCGRG	1 cut(s)	963
AvaII	GGWCC	1 cut(s)	298
BalI	TGGCCA	2 cut(s)	796, 867
BbrPI	CACGTG	1 cut(s)	145
BbsI	GAAGAC	1 cut(s)	376
Bbv12I	GWGCWC	1 cut(s)	144
BbvI	GCAGC	6 cut(s)	137, 290, 502, 640, 648, 742
BccI	CCATC	5 cut(s)	161, 182, 197, 357, 719
BciT130I	CCWGG	1 cut(s)	1254
BciVI	GTATCC	1 cut(s)	1034
BclI	TGATCA	1 cut(s)	391
BcoDI	GTCTC	3 cut(s)	1092, 1149, 1295
BfaI	CTAG	1 cut(s)	879
BfmI	CTRYAG	2 cut(s)	516, 662
BfrI	CTTAAG	1 cut(s)	1300
BfuI	GTATCC	1 cut(s)	1034
BisI	GCNGC	6 cut(s)	126, 279, 516, 654, 662, 731
BlpI	GCTNAGC	4 cut(s)	34, 65, 138, 972
BlsI	GCNGC	6 cut(s)	127, 280, 517, 655, 663, 732
Bme1390I	CCNGG	1 cut(s)	1254
Bme18I	GGWCC	1 cut(s)	298
BmeT110I	CYCGRG	1 cut(s)	963
BmgT120I	GGNCC	2 cut(s)	298, 755
BmiI	GGNNCC	5 cut(s)	213, 299, 300, 315, 1307
BmrFI	CCNGG	1 cut(s)	1254
BmsI	GCATC	6 cut(s)	344, 376, 411, 640, 667, 958
BpiI	GAAGAC	1 cut(s)	376
BpmI	CTGGAG	1 cut(s)	804
Bpu10I	CCTNAGC	2 cut(s)	163, 952
Bpu1102I	GCTNAGC	4 cut(s)	34, 65, 138, 972
BpuEI	CTTGAG	2 cut(s)	437, 1374
Bsa29I	ATCGAT	1 cut(s)	1279
BsaAI	YACGTR	2 cut(s)	145, 1408
BsaBI	GATNNNNATC	1 cut(s)	831
BsaI	GGTCTC	1 cut(s)	1149
BsaWI	WCCGGW	1 cut(s)	1013
Bsc4I	CCNNNNNNNGG	4 cut(s)	294, 628, 1020, 1302
Bse118I	RCCGGY	1 cut(s)	872
Bse1I	ACTGG	3 cut(s)	274, 797, 868
Bse8I	GATNNNNATC	1 cut(s)	831
BseBI	CCWGG	1 cut(s)	1254
BseCI	ATCGAT	1 cut(s)	1279
BseGI	GGATG	9 cut(s)	91, 278, 368, 426, 682, 910, 930, 1080, 1363
BseJI	GATNNNNATC	1 cut(s)	831
BseLI	CCNNNNNNNGG	4 cut(s)	294, 628, 1020, 1302
BseMII	CTCAG	6 cut(s)	56, 98, 129, 177, 420, 986
BseNI	ACTGG	3 cut(s)	274, 797, 868
BseXI	GCAGC	6 cut(s)	137, 290, 502, 640, 648, 742
BseYI	CCCAGC	2 cut(s)	930, 1062
BsgI	GTGCAG	2 cut(s)	297, 366
Bsh1236I	CGCG	1 cut(s)	432
BshFI	GGCC	5 cut(s)	192, 246, 756, 796, 867
BshVI	ATCGAT	1 cut(s)	1279
BsiHKAI	GWGCWC	1 cut(s)	144
BsiHKCI	CYCGRG	1 cut(s)	963
BsiSI	CCGG	2 cut(s)	873, 1014
BslFI	GGGAC	1 cut(s)	311
BslI	CCNNNNNNNGG	4 cut(s)	294, 628, 1020, 1302
BsmAI	GTCTC	3 cut(s)	1092, 1149, 1295
BsmBI	CGTCTC	1 cut(s)	1295
BsmFI	GGGAC	1 cut(s)	311
BsmI	GAATGC	1 cut(s)	766
BsnI	GGCC	5 cut(s)	192, 246, 756, 796, 867
Bso31I	GGTCTC	1 cut(s)	1149
BsoBI	CYCGRG	1 cut(s)	963
Bsp1286I	GDGCHC	1 cut(s)	144
Bsp143I	GATC	5 cut(s)	391, 623, 778, 832, 1276
Bsp1720I	GCTNAGC	4 cut(s)	34, 65, 138, 972
BspACI	CCGC	3 cut(s)	430, 458, 1099
BspANI	GGCC	5 cut(s)	192, 246, 756, 796, 867
BspCNI	CTCAG	6 cut(s)	57, 99, 130, 176, 419, 985
BspDI	ATCGAT	1 cut(s)	1279
BspFNI	CGCG	1 cut(s)	432
BspHI	TCATGA	2 cut(s)	837, 942
BspLI	GGNNCC	5 cut(s)	213, 299, 300, 315, 1307
BspMAI	CTGCAG	2 cut(s)	520, 666
BspQI	GCTCTTC	2 cut(s)	169, 1361
BspTI	CTTAAG	1 cut(s)	1300
BspTNI	GGTCTC	1 cut(s)	1149
BsrFI	RCCGGY	1 cut(s)	872
BsrI	ACTGG	3 cut(s)	274, 797, 868
BssAI	RCCGGY	1 cut(s)	872
BssMI	GATC	5 cut(s)	391, 623, 778, 832, 1276
BssNAI	GTATAC	1 cut(s)	787
Bst1107I	GTATAC	1 cut(s)	787
Bst2UI	CCWGG	1 cut(s)	1254
Bst4CI	ACNGT	3 cut(s)	293, 531, 593
Bst6I	CTCTTC	5 cut(s)	169, 559, 1113, 1191, 1361
BstAFI	CTTAAG	1 cut(s)	1300
BstAPI	GCANNNNNTGC	2 cut(s)	234, 815
BstBAI	YACGTR	2 cut(s)	145, 1408
BstC8I	GCNNGC	1 cut(s)	493
BstF5I	GGATG	9 cut(s)	91, 278, 368, 426, 682, 910, 930, 1080, 1363
BstFNI	CGCG	1 cut(s)	432
BstKTI	GATC	5 cut(s)	394, 626, 781, 835, 1279
BstMAI	GTCTC	3 cut(s)	1092, 1149, 1295
BstMBI	GATC	5 cut(s)	391, 623, 778, 832, 1276
BstMWI	GCNNNNNNNGC	8 cut(s)	134, 234, 243, 252, 284, 421, 524, 815
BstNI	CCWGG	1 cut(s)	1254
BstNSI	RCATGY	2 cut(s)	335, 640
BstSCI	CCNGG	1 cut(s)	1252
BstSFI	CTRYAG	2 cut(s)	516, 662
BstUI	CGCG	1 cut(s)	432
BstV1I	GCAGC	6 cut(s)	137, 290, 502, 640, 648, 742
BstV2I	GAAGAC	1 cut(s)	376
BstZ17I	GTATAC	1 cut(s)	787
Bsu15I	ATCGAT	1 cut(s)	1279
BsuI	GTATCC	1 cut(s)	1034
BsuRI	GGCC	5 cut(s)	192, 246, 756, 796, 867
BsuTUI	ATCGAT	1 cut(s)	1279
BtsCI	GGATG	9 cut(s)	91, 278, 368, 426, 682, 910, 930, 1080, 1363
BtsIMutI	CAGTG	4 cut(s)	298, 462, 527, 861
Cac8I	GCNNGC	1 cut(s)	493
CaiI	CAGNNNCTG	1 cut(s)	1259
CciI	TCATGA	2 cut(s)	837, 942
Cfr10I	RCCGGY	1 cut(s)	872
Cfr13I	GGNCC	2 cut(s)	298, 755
ClaI	ATCGAT	1 cut(s)	1279
Csp6I	GTAC	2 cut(s)	876, 916
CviAII	CATG	8 cut(s)	170, 248, 332, 637, 838, 869, 943, 1176
CviQI	GTAC	2 cut(s)	876, 916
DpnI	GATC	5 cut(s)	393, 625, 780, 834, 1278
DpnII	GATC	5 cut(s)	391, 623, 778, 832, 1276
EaeI	YGGCCR	2 cut(s)	794, 865
Eam1104I	CTCTTC	5 cut(s)	169, 559, 1113, 1191, 1361
EarI	CTCTTC	5 cut(s)	169, 559, 1113, 1191, 1361
Eco31I	GGTCTC	1 cut(s)	1149
Eco47I	GGWCC	1 cut(s)	298
Eco57I	CTGAAG	5 cut(s)	55, 486, 755, 1293, 1316
Eco72I	CACGTG	1 cut(s)	145
Eco88I	CYCGRG	1 cut(s)	963
EcoO109I	RGGNCCY	1 cut(s)	298
EcoRII	CCWGG	1 cut(s)	1252
EcoT22I	ATGCAT	2 cut(s)	337, 1177
Esp3I	CGTCTC	1 cut(s)	1295
FaeI	CATG	8 cut(s)	173, 251, 335, 640, 841, 872, 946, 1179
FaqI	GGGAC	1 cut(s)	311
FatI	CATG	8 cut(s)	169, 247, 331, 636, 837, 868, 942, 1175
FbaI	TGATCA	1 cut(s)	391
FblI	GTMKAC	2 cut(s)	533, 786
Fnu4HI	GCNGC	6 cut(s)	126, 279, 516, 654, 662, 731
FokI	GGATG	9 cut(s)	98, 285, 375, 413, 689, 917, 937, 1067, 1370
Fsp4HI	GCNGC	6 cut(s)	126, 279, 516, 654, 662, 731
FspBI	CTAG	1 cut(s)	879
GluI	GCNGC	6 cut(s)	126, 279, 516, 654, 662, 731
GsaI	CCCAGC	2 cut(s)	934, 1066
GsuI	CTGGAG	1 cut(s)	804
HaeIII	GGCC	5 cut(s)	192, 246, 756, 796, 867
HapII	CCGG	2 cut(s)	873, 1014
Hin1II	CATG	8 cut(s)	173, 251, 335, 640, 841, 872, 946, 1179
HincII	GTYRAC	2 cut(s)	523, 534
HindII	GTYRAC	2 cut(s)	523, 534
HindIII	AAGCTT	2 cut(s)	16, 989
HinfI	GANTC	4 cut(s)	75, 882, 908, 1338
HpaI	GTTAAC	1 cut(s)	523
HpaII	CCGG	2 cut(s)	873, 1014
HphI	GGTGA	5 cut(s)	549, 702, 1155, 1192, 1285
Hpy166II	GTNNAC	4 cut(s)	523, 534, 787, 916
Hpy188I	TCNGA	6 cut(s)	74, 452, 623, 676, 1040, 1224
Hpy188III	TCNNGA	7 cut(s)	200, 722, 838, 943, 963, 965, 1144
Hpy8I	GTNNAC	4 cut(s)	523, 534, 787, 916
HpyAV	CCTTC	7 cut(s)	202, 461, 570, 733, 746, 1170, 1355
HpyCH4III	ACNGT	3 cut(s)	293, 531, 593
HpyCH4IV	ACGT	2 cut(s)	144, 1407
HpyCH4V	TGCA	9 cut(s)	228, 278, 335, 347, 518, 607, 664, 1134, 1175
HpyF10VI	GCNNNNNNNGC	8 cut(s)	134, 234, 243, 252, 284, 421, 524, 815
HpySE526I	ACGT	2 cut(s)	144, 1407
Hsp92II	CATG	8 cut(s)	173, 251, 335, 640, 841, 872, 946, 1179
KflI	GGGWCCC	1 cut(s)	298
Ksp22I	TGATCA	1 cut(s)	391
KspAI	GTTAAC	1 cut(s)	523
Kzo9I	GATC	5 cut(s)	391, 623, 778, 832, 1276
LguI	GCTCTTC	2 cut(s)	169, 1361
LmnI	GCTCC	5 cut(s)	103, 114, 313, 823, 1268
Lsp1109I	GCAGC	6 cut(s)	137, 290, 502, 640, 648, 742
LweI	GCATC	6 cut(s)	344, 376, 411, 640, 667, 958
MaeI	CTAG	1 cut(s)	879
MaeII	ACGT	2 cut(s)	144, 1407
MaeIII	GTNAC	3 cut(s)	460, 703, 1408
MalI	GATC	5 cut(s)	393, 625, 780, 834, 1278
MboI	GATC	5 cut(s)	391, 623, 778, 832, 1276
MhlI	GDGCHC	1 cut(s)	144
MlsI	TGGCCA	2 cut(s)	796, 867
MluNI	TGGCCA	2 cut(s)	796, 867
MlyI	GAGTC	3 cut(s)	69, 917, 1332
MmeI	TCCRAC	2 cut(s)	92, 430
MnlI	CCTC	7 cut(s)	236, 414, 718, 1010, 1185, 1192, 1307
Mox20I	TGGCCA	2 cut(s)	796, 867
Mph1103I	ATGCAT	2 cut(s)	337, 1177
MscI	TGGCCA	2 cut(s)	796, 867
MseI	TTAA	5 cut(s)	54, 266, 522, 1301, 1414
Msp20I	TGGCCA	2 cut(s)	796, 867
MspA1I	CMGCKG	1 cut(s)	733
MspCI	CTTAAG	1 cut(s)	1300
MspI	CCGG	2 cut(s)	873, 1014
MspR9I	CCNGG	1 cut(s)	1254
Mva1269I	GAATGC	1 cut(s)	766
MvaI	CCWGG	1 cut(s)	1254
MvnI	CGCG	1 cut(s)	432
MwoI	GCNNNNNNNGC	8 cut(s)	134, 234, 243, 252, 284, 421, 524, 815
NdeII	GATC	5 cut(s)	391, 623, 778, 832, 1276
NlaIII	CATG	8 cut(s)	173, 251, 335, 640, 841, 872, 946, 1179
NlaIV	GGNNCC	5 cut(s)	213, 299, 300, 315, 1307
NmuCI	GTSAC	2 cut(s)	460, 1408
NsiI	ATGCAT	2 cut(s)	337, 1177
NspI	RCATGY	2 cut(s)	335, 640
PaeR7I	CTCGAG	1 cut(s)	963
PagI	TCATGA	2 cut(s)	837, 942
PciI	ACATGT	1 cut(s)	636
PciSI	GCTCTTC	2 cut(s)	169, 1361
PcsI	WCGNNNNNNNCGW	1 cut(s)	1285
PctI	GAATGC	1 cut(s)	766
PfeI	GAWTC	1 cut(s)	882
PkrI	GCNGC	6 cut(s)	127, 280, 517, 655, 663, 732
PleI	GAGTC	3 cut(s)	69, 916, 1332
PmaCI	CACGTG	1 cut(s)	145
PmlI	CACGTG	1 cut(s)	145
PpsI	GAGTC	3 cut(s)	69, 916, 1332
Ppu21I	YACGTR	2 cut(s)	145, 1408
PpuMI	RGGWCCY	1 cut(s)	298
PscI	ACATGT	1 cut(s)	636
Psp5II	RGGWCCY	1 cut(s)	298
Psp6I	CCWGG	1 cut(s)	1252
PspCI	CACGTG	1 cut(s)	145
PspFI	CCCAGC	2 cut(s)	930, 1062
PspGI	CCWGG	1 cut(s)	1252
PspN4I	GGNNCC	5 cut(s)	213, 299, 300, 315, 1307
PspPI	GGNCC	2 cut(s)	298, 755
PspPPI	RGGWCCY	1 cut(s)	298
PstI	CTGCAG	2 cut(s)	520, 666
PstNI	CAGNNNCTG	1 cut(s)	1259
PvuII	CAGCTG	1 cut(s)	733
RsaI	GTAC	2 cut(s)	877, 917
RsaNI	GTAC	2 cut(s)	876, 916
SalI	GTCGAC	1 cut(s)	532
SapI	GCTCTTC	2 cut(s)	169, 1361
SaqAI	TTAA	5 cut(s)	54, 266, 522, 1301, 1414
SatI	GCNGC	6 cut(s)	126, 279, 516, 654, 662, 731
Sau3AI	GATC	5 cut(s)	391, 623, 778, 832, 1276
Sau96I	GGNCC	2 cut(s)	298, 755
SchI	GAGTC	3 cut(s)	69, 917, 1332
ScrFI	CCNGG	1 cut(s)	1254
SduI	GDGCHC	1 cut(s)	144
SfaNI	GCATC	6 cut(s)	344, 376, 411, 640, 667, 958
SfcI	CTRYAG	2 cut(s)	516, 662
Sfr274I	CTCGAG	1 cut(s)	963
SinI	GGWCC	1 cut(s)	298
SlaI	CTCGAG	1 cut(s)	963
SmlI	CTYRAG	4 cut(s)	416, 963, 1300, 1389
SmoI	CTYRAG	4 cut(s)	416, 963, 1300, 1389
SsiI	CCGC	3 cut(s)	430, 458, 1099
SspI	AATATT	1 cut(s)	1150
SspMI	CTAG	1 cut(s)	879
StyD4I	CCNGG	1 cut(s)	1252
TaaI	ACNGT	3 cut(s)	293, 531, 593
TaiI	ACGT	2 cut(s)	147, 1410
TaqI	TCGA	4 cut(s)	370, 533, 964, 1279
TfiI	GAWTC	1 cut(s)	882
Tru1I	TTAA	5 cut(s)	54, 266, 522, 1301, 1414
Tru9I	TTAA	5 cut(s)	54, 266, 522, 1301, 1414
TscAI	CASTG	4 cut(s)	298, 469, 534, 868
TseFI	GTSAC	2 cut(s)	460, 1408
TseI	GCWGC	6 cut(s)	125, 278, 515, 653, 661, 730
Tsp45I	GTSAC	2 cut(s)	460, 1408
TspDTI	ATGAA	3 cut(s)	428, 1230, 1374
TspGWI	ACGGA	1 cut(s)	626
TspRI	CASTG	4 cut(s)	298, 469, 534, 868
Vha464I	CTTAAG	1 cut(s)	1300
VpaK11BI	GGWCC	1 cut(s)	298
XapI	RAATTY	3 cut(s)	435, 1069, 1262
XceI	RCATGY	2 cut(s)	335, 640
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1173
XhoI	CTCGAG	1 cut(s)	963
XmiI	GTMKAC	2 cut(s)	533, 786
XspI	CTAG	1 cut(s)	879
Zsp2I	ATGCAT	2 cut(s)	337, 1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.