Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001908

Physical Location & Seq

Genomic Coordinates Reverse (-)

4643726 .. 4646786

Gene Structure 3061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1035800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCTAAGGCACAAAGCTCCTACGCTGTTATCCTGGCATTTTCTTTACTTGTTAATCTATTGGTATATACGGATGGAGAGCGGGAACTGAGTTGGAGCAAAAGAGCAGCACAAGAAGCTGAGCAGGTGGCCGCCATCTCTTGCTCAGGCCATGGAAGAGCCTACTTGGACGGGTTGGTTCTTTATGGAATCAAACCTGTTTGTGAGTGCAATCCTTGCTATGGAGGTCCTGACTGCTCTACGTTTTCACGTAATTGTGCAGCAAATGCCGAAAATGGGGATCCATATTTCTTGGAGCCATTTTGGATGCAACGTGCAGCTAAAAGTGCAATTCTAGTAGCAGGGTGGCATAGAATGGGGTATAGCTTTCCTGATGGTTCTTACATATCAACACAGCTCGAGGCGAGCATCCATAAAGTACATGCCGTAGTTGGAAACGCGGTCACTAAAGGAAAATATATTATTTTTGGTGCTGGCTCAACCCACCTTCTCAACGCTGCAGTTCACGCACTTGCTCTTGAGAATAATTCCTCCTCACCTGCAACTGTTGTTGCTTCAATTCCTTACTACAGGCTTTACAAGACACAAACGGAATTTTTCAGATCAGTCGACTATCAGTTTGAAGGAGATGCAACATCATTGCAGAACATTTCAGATGCAGGCAACGTTATTGAGTTTGTAACCTCACCAAACAATCCAGATGGGAAGCTGAATAAGGCAGTACTTCAGGGCCCCAATTCCTCTGCGATCTACGATCGTGTCTATTATTGGCCACATTTTACAGCGATTCCAACTGCAGCAAATGATGATCTAATGATATTTTCACTTTCCAAGCTCACTGGCCATGCTGGTACAAGATTTGGATGGGCAGTGGTAAAGAATGAGTCTGTTTATCAAAGAATGACGGAATATATTAGTGAAAGCAGCAATGGCATTTCTCGGGATGCTCAGCTAAGGGCTTTGAAACTTCTGAATGTAGTTCTTGAAACTGGAGGCAGGGAAATCTTTGAATTCGGATACGACACTATGAAGAACCGCTGGGAAAAATTGACCAACACCCTGTCTGTTTCCAATCGCTTTTCTCTACAGAAAATTGAACCCCAATATTGCACTTATTTTCAGAAAATTAGAGAACCCTCACCAGCTTATGCGTGGGTGAAGTGCGAGAGGGAAGAAGATAAAGATTGCTACAAAATACTGGAAGAAGCAAAAGTTAATGGGCGTAGAGGTAGCGTGTATGCAGCTGAAGAGCGCTATGTCCGTCTCAGCCTAATAAGGAGCCAAGATGAGTTTGACATACTAATTCAGCGACTAAGCCAGTTGGTCAAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alliinase_C EGF_alliinase

Protein Analysis

445

Amino Acids

49.65

Weight (kDa)

6.48

Isoelectric Point (pI)

38.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EGF_alliinase	PF04863	31 - 86	6.3e-29	Alliinase EGF-like domain
Alliinase_C	PF04864	88 - 442	6.3e-150	Allinase
Aminotran_1_2	PF00155	137 - 438	1.5e-08	Aminotransferase class I and II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34040 AT1G34040 AT1G34060 AT1G34060
fragaria_vesca	FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010 FvH4_2g13010
malus_domestica	MD05G1033800.v1.1 MD10G1035100.v1.1 MD10G1035600.v1.1 MD10G1035700.v1.1 MD10G1035800.v1.1
prunus_persica	Prupe.8G041100_v2.0.a1 Prupe.8G041200_v2.0.a1 Prupe.8G041300_v2.0.a1 Prupe.8G041600_v2.0.a1 Prupe.8G041600_v2.0.a1
pyrus_communis	pycom05g02440 pycom10g02410 pycom10g02420
rosa_chinensis	RchiOBHm_Chr6g0274171 RchiOBHm_Chr6g0274191
rosa_laevigata	RLG00000013573 RLG00000013574 RLG00000013575
rosa_multiflora	Rmu_co8325345.1_g000001 Rmu_co8467135.1_g000001 Rmu_sc0001939.1_g000013 Rmu_sc0001939.1_g000014 Rmu_sc0001939.1_g000015
rosa_roxburghii	Rroxscaffold_7G00194440 Rroxscaffold_7G00194490
rosa_rugosa	Rorug06G0079700
rosa_samantha	Rh6AG194800 Rh6AG194900 Rh6BG199000 Rh6BG199200 Rh6CG199400 Rh6DG190700 Rh6DG190900
rosa_wichuraiana	Rw6G017030 Rw6G017040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	115, 547
Acc36I	ACCTGC	2 cut(s)	115, 547
AccBSI	CCGCTC	1 cut(s)	82
AccI	GTMKAC	1 cut(s)	609
AccII	CGCG	1 cut(s)	440
AciI	CCGC	4 cut(s)	82, 132, 440, 1036
AclI	AACGTT	1 cut(s)	666
AclWI	GGATC	2 cut(s)	275, 288
AcoI	YGGCCR	3 cut(s)	129, 770, 841
AcsI	RAATTY	2 cut(s)	593, 1010
AcuI	CTGAAG	2 cut(s)	710, 1266
AfaI	GTAC	3 cut(s)	420, 723, 853
AfeI	AGCGCT	1 cut(s)	1253
AfiI	CCNNNNNNNGG	2 cut(s)	221, 276
AgsI	TTSAA	6 cut(s)	558, 623, 964, 986, 1010, 1097
AjnI	CCWGG	1 cut(s)	33
Alw26I	GTCTC	1 cut(s)	1268
AlwI	GGATC	2 cut(s)	275, 288
Ama87I	CYCGRG	2 cut(s)	398, 939
Aor51HI	AGCGCT	1 cut(s)	1253
AoxI	GGCC	5 cut(s)	129, 148, 730, 770, 841
ApaI	GGGCCC	1 cut(s)	734
ApeKI	GCWGC	7 cut(s)	107, 260, 317, 497, 797, 924, 1241
ApoI	RAATTY	2 cut(s)	593, 1010
ArsI	GACNNNNNNTTYG	2 cut(s)	602, 634
AspLEI	GCGC	1 cut(s)	1254
AspS9I	GGNCC	3 cut(s)	227, 730, 731
AsuHPI	GGTGA	4 cut(s)	528, 678, 1131, 1168
AvaI	CYCGRG	2 cut(s)	398, 939
AvaII	GGWCC	1 cut(s)	227
BaeGI	GKGCMC	1 cut(s)	734
BalI	TGGCCA	2 cut(s)	772, 843
BamHI	GGATCC	1 cut(s)	280
BanII	GRGCYC	1 cut(s)	734
BbvI	GCAGC	7 cut(s)	119, 272, 329, 484, 809, 936, 1253
BccI	CCATC	5 cut(s)	68, 143, 368, 695, 858
BceAI	ACGGC	1 cut(s)	410
BciT130I	CCWGG	1 cut(s)	35
BciVI	GTATCC	1 cut(s)	1010
BcoDI	GTCTC	1 cut(s)	1268
BfaI	CTAG	1 cut(s)	335
BfmI	CTRYAG	4 cut(s)	498, 568, 795, 1085
BfoI	RGCGCY	1 cut(s)	1255
BfuAI	ACCTGC	2 cut(s)	115, 547
BfuI	GTATCC	1 cut(s)	1010
BisI	GCNGC	8 cut(s)	108, 132, 261, 318, 498, 798, 925, 1242
BlpI	GCTNAGC	2 cut(s)	120, 948
BlsI	GCNGC	8 cut(s)	109, 133, 262, 319, 499, 799, 926, 1243
BmcAI	AGTACT	1 cut(s)	723
Bme1390I	CCNGG	1 cut(s)	35
Bme18I	GGWCC	1 cut(s)	227
BmeT110I	CYCGRG	2 cut(s)	398, 939
BmgT120I	GGNCC	3 cut(s)	227, 730, 731
BmiI	GGNNCC	5 cut(s)	282, 297, 732, 733, 1280
BmrFI	CCNGG	1 cut(s)	35
BmsI	GCATC	5 cut(s)	297, 417, 619, 646, 934
BpmI	CTGGAG	1 cut(s)	1011
Bpu10I	CCTNAGC	3 cut(s)	6, 145, 953
Bpu1102I	GCTNAGC	2 cut(s)	120, 948
BpuEI	CTTGAG	1 cut(s)	539
BsaAI	YACGTR	1 cut(s)	251
BsaJI	CCNNGG	1 cut(s)	151
Bsc4I	CCNNNNNNNGG	2 cut(s)	221, 276
Bse1I	ACTGG	4 cut(s)	844, 994, 1203, 1318
Bse3DI	GCAATG	2 cut(s)	638, 934
BseBI	CCWGG	1 cut(s)	35
BseDI	CCNNGG	1 cut(s)	151
BseGI	GGATG	5 cut(s)	79, 312, 408, 869, 949
BseLI	CCNNNNNNNGG	2 cut(s)	221, 276
BseMI	GCAATG	2 cut(s)	638, 934
BseMII	CTCAG	5 cut(s)	80, 111, 159, 962, 1279
BseNI	ACTGG	4 cut(s)	844, 994, 1203, 1318
BseRI	GAGGAG	1 cut(s)	523
BseSI	GKGCMC	1 cut(s)	734
BseXI	GCAGC	7 cut(s)	119, 272, 329, 484, 809, 936, 1253
BseYI	CCCAGC	1 cut(s)	1038
BsgI	GTGCAG	2 cut(s)	279, 336
Bsh1236I	CGCG	1 cut(s)	440
Bsh1285I	CGRYCG	1 cut(s)	757
BshFI	GGCC	5 cut(s)	131, 150, 732, 772, 843
BsiEI	CGRYCG	1 cut(s)	757
BsiHKCI	CYCGRG	2 cut(s)	398, 939
BslI	CCNNNNNNNGG	2 cut(s)	221, 276
BsmAI	GTCTC	1 cut(s)	1268
BsmBI	CGTCTC	1 cut(s)	1268
BsnI	GGCC	5 cut(s)	131, 150, 732, 772, 843
BsoBI	CYCGRG	2 cut(s)	398, 939
Bsp120I	GGGCCC	1 cut(s)	730
Bsp1286I	GDGCHC	1 cut(s)	734
Bsp143I	GATC	5 cut(s)	280, 602, 747, 754, 808
Bsp1720I	GCTNAGC	2 cut(s)	120, 948
Bsp19I	CCATGG	1 cut(s)	151
BspACI	CCGC	4 cut(s)	82, 132, 440, 1036
BspANI	GGCC	5 cut(s)	131, 150, 732, 772, 843
BspCNI	CTCAG	5 cut(s)	81, 112, 158, 961, 1278
BspFNI	CGCG	1 cut(s)	440
BspLI	GGNNCC	5 cut(s)	282, 297, 732, 733, 1280
BspMAI	CTGCAG	2 cut(s)	502, 799
BspMI	ACCTGC	2 cut(s)	115, 547
BspPI	GGATC	2 cut(s)	275, 288
BspQI	GCTCTTC	2 cut(s)	151, 1242
BsrBI	CCGCTC	1 cut(s)	82
BsrDI	GCAATG	2 cut(s)	638, 934
BsrI	ACTGG	4 cut(s)	844, 994, 1203, 1318
BssECI	CCNNGG	1 cut(s)	151
BssMI	GATC	5 cut(s)	280, 602, 747, 754, 808
BssT1I	CCWWGG	1 cut(s)	151
Bst2UI	CCWGG	1 cut(s)	35
Bst4CI	ACNGT	1 cut(s)	547
Bst6I	CTCTTC	2 cut(s)	151, 1242
BstAPI	GCANNNNNTGC	2 cut(s)	216, 266
BstBAI	YACGTR	1 cut(s)	251
BstC8I	GCNNGC	3 cut(s)	406, 475, 661
BstDEI	CTNAG	8 cut(s)	6, 89, 120, 145, 948, 953, 1265, 1313
BstDSI	CCRYGG	1 cut(s)	151
BstF5I	GGATG	5 cut(s)	79, 312, 408, 869, 949
BstFNI	CGCG	1 cut(s)	440
BstH2I	RGCGCY	1 cut(s)	1255
BstHHI	GCGC	1 cut(s)	1254
BstKTI	GATC	5 cut(s)	283, 605, 750, 757, 811
BstMAI	GTCTC	1 cut(s)	1268
BstMBI	GATC	5 cut(s)	280, 602, 747, 754, 808
BstMCI	CGRYCG	1 cut(s)	757
BstMWI	GCNNNNNNNGC	6 cut(s)	116, 216, 266, 326, 506, 930
BstNI	CCWGG	1 cut(s)	35
BstNSI	RCATGY	1 cut(s)	425
BstSCI	CCNGG	1 cut(s)	33
BstSFI	CTRYAG	4 cut(s)	498, 568, 795, 1085
BstSLI	GKGCMC	1 cut(s)	734
BstUI	CGCG	1 cut(s)	440
BstV1I	GCAGC	7 cut(s)	119, 272, 329, 484, 809, 936, 1253
BstX2I	RGATCY	1 cut(s)	280
BstYI	RGATCY	1 cut(s)	280
BsuI	GTATCC	1 cut(s)	1010
BsuRI	GGCC	5 cut(s)	131, 150, 732, 772, 843
BtgI	CCRYGG	1 cut(s)	151
BtsCI	GGATG	5 cut(s)	79, 312, 408, 869, 949
BtsI	GCAGTG	1 cut(s)	876
BtsIMutI	CAGTG	2 cut(s)	837, 876
BveI	ACCTGC	2 cut(s)	115, 547
Cac8I	GCNNGC	3 cut(s)	406, 475, 661
CfoI	GCGC	1 cut(s)	1254
Cfr13I	GGNCC	3 cut(s)	227, 730, 731
Csp6I	GTAC	3 cut(s)	419, 722, 852
CviAII	CATG	3 cut(s)	152, 422, 845
CviQI	GTAC	3 cut(s)	419, 722, 852
DdeI	CTNAG	8 cut(s)	6, 89, 120, 145, 948, 953, 1265, 1313
DpnI	GATC	5 cut(s)	282, 604, 749, 756, 810
DpnII	GATC	5 cut(s)	280, 602, 747, 754, 808
EaeI	YGGCCR	3 cut(s)	129, 770, 841
Eam1104I	CTCTTC	2 cut(s)	151, 1242
EarI	CTCTTC	2 cut(s)	151, 1242
Eco130I	CCWWGG	1 cut(s)	151
Eco24I	GRGCYC	1 cut(s)	734
Eco47I	GGWCC	1 cut(s)	227
Eco47III	AGCGCT	1 cut(s)	1253
Eco57I	CTGAAG	2 cut(s)	710, 1266
Eco88I	CYCGRG	2 cut(s)	398, 939
EcoO109I	RGGNCCY	3 cut(s)	227, 730, 731
EcoRI	GAATTC	1 cut(s)	1010
EcoRII	CCWGG	1 cut(s)	33
EcoT14I	CCWWGG	1 cut(s)	151
EcoT38I	GRGCYC	1 cut(s)	734
ErhI	CCWWGG	1 cut(s)	151
Esp3I	CGTCTC	1 cut(s)	1268
FaeI	CATG	3 cut(s)	155, 425, 848
FatI	CATG	3 cut(s)	151, 421, 844
FauI	CCCGC	1 cut(s)	75
FblI	GTMKAC	1 cut(s)	609
Fnu4HI	GCNGC	8 cut(s)	108, 132, 261, 318, 498, 798, 925, 1242
FokI	GGATG	5 cut(s)	86, 319, 395, 876, 956
FriOI	GRGCYC	1 cut(s)	734
Fsp4HI	GCNGC	8 cut(s)	108, 132, 261, 318, 498, 798, 925, 1242
FspBI	CTAG	1 cut(s)	335
GlaI	GCGC	1 cut(s)	1253
GluI	GCNGC	8 cut(s)	108, 132, 261, 318, 498, 798, 925, 1242
GsaI	CCCAGC	1 cut(s)	1042
GsuI	CTGGAG	1 cut(s)	1011
HaeII	RGCGCY	1 cut(s)	1255
HaeIII	GGCC	5 cut(s)	131, 150, 732, 772, 843
HhaI	GCGC	1 cut(s)	1254
Hin1II	CATG	3 cut(s)	155, 425, 848
Hin6I	GCGC	1 cut(s)	1252
HinP1I	GCGC	1 cut(s)	1252
HincII	GTYRAC	1 cut(s)	610
HindII	GTYRAC	1 cut(s)	610
HinfI	GANTC	3 cut(s)	189, 787, 884
HphI	GGTGA	4 cut(s)	528, 678, 1131, 1168
Hpy166II	GTNNAC	2 cut(s)	505, 610
Hpy188I	TCNGA	5 cut(s)	602, 655, 972, 1016, 1122
Hpy188III	TCNNGA	6 cut(s)	230, 371, 518, 698, 941, 983
Hpy8I	GTNNAC	2 cut(s)	505, 610
HpyAV	CCTTC	2 cut(s)	497, 617
HpyCH4III	ACNGT	1 cut(s)	547
HpyCH4IV	ACGT	4 cut(s)	242, 250, 313, 666
HpyF10VI	GCNNNNNNNGC	6 cut(s)	116, 216, 266, 326, 506, 930
HpyF3I	CTNAG	8 cut(s)	6, 89, 120, 145, 948, 953, 1265, 1313
HpySE526I	ACGT	4 cut(s)	242, 250, 313, 666
Hsp92II	CATG	3 cut(s)	155, 425, 848
HspAI	GCGC	1 cut(s)	1252
Kzo9I	GATC	5 cut(s)	280, 602, 747, 754, 808
LguI	GCTCTTC	2 cut(s)	151, 1242
LmnI	GCTCC	4 cut(s)	23, 96, 295, 1278
Lsp1109I	GCAGC	7 cut(s)	119, 272, 329, 484, 809, 936, 1253
LweI	GCATC	5 cut(s)	297, 417, 619, 646, 934
MaeI	CTAG	1 cut(s)	335
MaeII	ACGT	4 cut(s)	242, 250, 313, 666
MaeIII	GTNAC	2 cut(s)	442, 679
MalI	GATC	5 cut(s)	282, 604, 749, 756, 810
MbiI	CCGCTC	1 cut(s)	82
MboI	GATC	5 cut(s)	280, 602, 747, 754, 808
MboII	GAAGA	6 cut(s)	168, 1042, 1184, 1187, 1214, 1259
MflI	RGATCY	1 cut(s)	280
MhlI	GDGCHC	1 cut(s)	734
MlsI	TGGCCA	2 cut(s)	772, 843
MluNI	TGGCCA	2 cut(s)	772, 843
MlyI	GAGTC	1 cut(s)	893
MmeI	TCCRAC	3 cut(s)	74, 412, 815
Mox20I	TGGCCA	2 cut(s)	772, 843
MscI	TGGCCA	2 cut(s)	772, 843
MseI	TTAA	2 cut(s)	54, 1215
Msp20I	TGGCCA	2 cut(s)	772, 843
MspA1I	CMGCKG	2 cut(s)	1038, 1244
MspR9I	CCNGG	1 cut(s)	35
MvaI	CCWGG	1 cut(s)	35
MvnI	CGCG	1 cut(s)	440
MwoI	GCNNNNNNNGC	6 cut(s)	116, 216, 266, 326, 506, 930
NcoI	CCATGG	1 cut(s)	151
NdeII	GATC	5 cut(s)	280, 602, 747, 754, 808
NlaIII	CATG	3 cut(s)	155, 425, 848
NlaIV	GGNNCC	5 cut(s)	282, 297, 732, 733, 1280
NmuCI	GTSAC	1 cut(s)	442
NspI	RCATGY	1 cut(s)	425
PaeR7I	CTCGAG	1 cut(s)	398
PaqCI	CACCTGC	2 cut(s)	115, 547
PciSI	GCTCTTC	2 cut(s)	151, 1242
PfeI	GAWTC	2 cut(s)	189, 787
PkrI	GCNGC	8 cut(s)	109, 133, 262, 319, 499, 799, 926, 1243
Ple19I	CGATCG	1 cut(s)	757
PleI	GAGTC	1 cut(s)	892
PpsI	GAGTC	1 cut(s)	892
Ppu21I	YACGTR	1 cut(s)	251
PpuMI	RGGWCCY	1 cut(s)	227
Psp1406I	AACGTT	1 cut(s)	666
Psp5II	RGGWCCY	1 cut(s)	227
Psp6I	CCWGG	1 cut(s)	33
PspFI	CCCAGC	1 cut(s)	1038
PspGI	CCWGG	1 cut(s)	33
PspN4I	GGNNCC	5 cut(s)	282, 297, 732, 733, 1280
PspOMI	GGGCCC	1 cut(s)	730
PspPI	GGNCC	3 cut(s)	227, 730, 731
PspPPI	RGGWCCY	1 cut(s)	227
PspXI	VCTCGAGB	1 cut(s)	398
PstI	CTGCAG	2 cut(s)	502, 799
PsuI	RGATCY	1 cut(s)	280
PvuI	CGATCG	1 cut(s)	757
PvuII	CAGCTG	1 cut(s)	1244
RsaI	GTAC	3 cut(s)	420, 723, 853
RsaNI	GTAC	3 cut(s)	419, 722, 852
SalI	GTCGAC	1 cut(s)	608
SapI	GCTCTTC	2 cut(s)	151, 1242
SaqAI	TTAA	2 cut(s)	54, 1215
SatI	GCNGC	8 cut(s)	108, 132, 261, 318, 498, 798, 925, 1242
Sau3AI	GATC	5 cut(s)	280, 602, 747, 754, 808
Sau96I	GGNCC	3 cut(s)	227, 730, 731
ScaI	AGTACT	1 cut(s)	723
SchI	GAGTC	1 cut(s)	893
ScrFI	CCNGG	1 cut(s)	35
SduI	GDGCHC	1 cut(s)	734
SfaNI	GCATC	5 cut(s)	297, 417, 619, 646, 934
SfcI	CTRYAG	4 cut(s)	498, 568, 795, 1085
Sfr274I	CTCGAG	1 cut(s)	398
SinI	GGWCC	1 cut(s)	227
SlaI	CTCGAG	1 cut(s)	398
SmlI	CTYRAG	2 cut(s)	398, 518
SmoI	CTYRAG	2 cut(s)	398, 518
SsiI	CCGC	4 cut(s)	82, 132, 440, 1036
SspI	AATATT	1 cut(s)	1106
SspMI	CTAG	1 cut(s)	335
StyD4I	CCNGG	1 cut(s)	33
StyI	CCWWGG	1 cut(s)	151
TaaI	ACNGT	1 cut(s)	547
TaiI	ACGT	4 cut(s)	245, 253, 316, 669
TaqI	TCGA	2 cut(s)	399, 609
TatI	WGTACW	2 cut(s)	418, 721
TauI	GCSGC	1 cut(s)	134
TfiI	GAWTC	2 cut(s)	189, 787
Tru1I	TTAA	2 cut(s)	54, 1215
Tru9I	TTAA	2 cut(s)	54, 1215
TscAI	CASTG	2 cut(s)	844, 876
TseFI	GTSAC	1 cut(s)	442
TseI	GCWGC	7 cut(s)	107, 260, 317, 497, 797, 924, 1241
Tsp45I	GTSAC	1 cut(s)	442
TspDTI	ATGAA	1 cut(s)	1043
TspGWI	ACGGA	4 cut(s)	86, 605, 920, 1250
TspRI	CASTG	2 cut(s)	844, 876
VpaK11BI	GGWCC	1 cut(s)	227
XapI	RAATTY	2 cut(s)	593, 1010
XceI	RCATGY	1 cut(s)	425
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1149
XhoI	CTCGAG	1 cut(s)	398
XmiI	GTMKAC	1 cut(s)	609
XspI	CTAG	1 cut(s)	335
ZrmI	AGTACT	1 cut(s)	723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.