MD11G1162100.v1.1

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002803
Physical Location & Seq
Forward (+)
16388823 .. 16392470
3648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1162100.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGCCTTATCCTCTTCAATCCCCCCAATTGCTTCGCTTCCACCGCTGCCGTTACACTAAAATGGCAACTTTACATTTACACAGCGCCCTAGCTAACCCGACGCCGTTCAAATCCACAAACACCCAGATTTCAACTCGGCGAGAGCCACCCAGGCGCCGAGCCAACTCGGTCAGGTGCGGCGAGGCAACTCAGGTCACCGGCGAGACCCACCGAGTCACGGTGAAGAACGGGAACGATTCTCTGGACATATGCCGAGTACTGAACGGAATGTGGCAGACGAGCGGTGGCTGGGGCAGAATCGACCGAGACGACGCCGTCGAGGCCATGCTCCGCTACGCAGACGCTGGCCTCTCCACCTTCGACATGGCAGACCACTATGGACCGGCTGAAGATCTATATGGCATCTTTATCAATCGAGTTCGTCAGGAGCGTCCACCAGAGTACTTGGAAAAGGTCAGAGGTCTTACGAAATGGGTGCCACCGCCTGTTAAGATGACAAGTAGTTTTGTAAGGGACAGTATTAATGTTTCACGAAAGAGAATGGACGTGGCTTCCTTGGACATGCTTCAGTTTCATTGGTGGGATTATGCAAATACTGGTTACCTTGATGCACTAAAACACCTTACAGATTTGAAAGAAGAAGGTAAAATTAAGACAGTCGCCTTGACAAATTTCGATACAGAGAGGCTACAGATAATCCTAGAAAATGAGATTCCTGTTGTAAGCAATCAGGTCCAGCATTCCATTGTCGATATGCGTCCTCAACAGAAAATGGCAGAGCTTTGTCAGCTCACTGGAGTCAAACTTATAACGTATGGGACAGTAATGGGCGGTCTATTATCTGAAAAGTTCCTCGATACCAACTTGAACATTCCTTTTGCCGGGCCTCCATTAAACACTCCCTCCCTCCAAAAGTACAAACGAATGGTTGATGCCTGGGGAGGTTGGAGTCTTTTCCAAGCTCTTCTACAGACACTCAAAGGCATCGCTACTAAACACGGAGTCTCCATTGCTAATGTTGCGGTGAAATACGTTCTAGATCAGCAAGCAGTTGCAGGATCAATGGTAGGTGTTAGACTCGGGCTGTCGGAACACATCCAAGACTGCAATGCTGTGTTCTCACTTGCTCTCGATGAGGAAGATGTAAACAGCATAAAAGAAGTCACAAAGAAAGGGAAGAATCTCCTTAAAGTGATTGGGGACTGTGGAGATGAATACCGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

409

Amino Acids

45.93

Weight (kDa)

8.87

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 87 - 388 1.4e-71 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 809
AasI GACNNNNNNGTC 1 cut(s) 314
AccB1I GGYRCC 2 cut(s) 153, 475
AccBSI CCGCTC 1 cut(s) 282
AciI CCGC 7 cut(s) 43, 177, 282, 331, 482, 831, 1022
AclWI GGATC 1 cut(s) 1066
AcsI RAATTY 1 cut(s) 670
AcuI CTGAAG 2 cut(s) 408, 551
AcyI GRCGYC 3 cut(s) 101, 154, 312
AfaI GTAC 3 cut(s) 258, 443, 917
AfiI CCNNNNNNNGG 2 cut(s) 217, 881
AgsI TTSAA 5 cut(s) 17, 109, 132, 634, 868
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 2 cut(s) 149, 935
AluBI AGCT 4 cut(s) 92, 781, 790, 962
AluI AGCT 4 cut(s) 92, 781, 790, 962
Alw26I GTCTC 3 cut(s) 197, 300, 1009
AlwI GGATC 1 cut(s) 1066
AlwNI CAGNNNCTG 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 1079
AoxI GGCC 3 cut(s) 321, 346, 884
ApeKI GCWGC 1 cut(s) 45
ApoI RAATTY 1 cut(s) 670
AseI ATTAAT 1 cut(s) 522
AspLEI GCGC 2 cut(s) 86, 156
AspS9I GGNCC 3 cut(s) 380, 733, 884
AsuC2I CCSGG 1 cut(s) 883
AsuHPI GGTGA 3 cut(s) 187, 232, 1036
AvaI CYCGRG 1 cut(s) 1079
AvaII GGWCC 2 cut(s) 380, 733
BanI GGYRCC 2 cut(s) 153, 475
BbvI GCAGC 1 cut(s) 32
BceAI ACGGC 3 cut(s) 33, 88, 299
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 2 cut(s) 151, 937
BcnI CCSGG 1 cut(s) 883
BcoDI GTCTC 3 cut(s) 197, 300, 1009
BfaI CTAG 3 cut(s) 89, 701, 1037
BfmI CTRYAG 2 cut(s) 689, 968
BfoI RGCGCY 2 cut(s) 87, 157
BglI GCCNNNNNGGC 2 cut(s) 151, 320
BglII AGATCT 1 cut(s) 391
BisI GCNGC 2 cut(s) 46, 178
BlsI GCNGC 2 cut(s) 47, 179
BmcAI AGTACT 2 cut(s) 258, 443
Bme1390I CCNGG 3 cut(s) 151, 883, 937
Bme18I GGWCC 2 cut(s) 380, 733
BmeT110I CYCGRG 1 cut(s) 1079
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 3 cut(s) 380, 733, 884
BmiI GGNNCC 2 cut(s) 155, 477
BmrFI CCNGG 3 cut(s) 151, 883, 937
BmsI GCATC 4 cut(s) 411, 598, 922, 993
BpmI CTGGAG 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 883
BsaHI GRCGYC 3 cut(s) 101, 154, 312
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 3 cut(s) 149, 555, 936
BsaXI ACNNNNNCTCC 4 cut(s) 887, 917, 989, 1019
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 881
Bse118I RCCGGY 3 cut(s) 197, 382, 1218
Bse1I ACTGG 2 cut(s) 601, 799
Bse3DI GCAATG 2 cut(s) 1008, 1114
BseBI CCWGG 2 cut(s) 151, 937
BseDI CCNNGG 3 cut(s) 149, 555, 936
BseGI GGATG 1 cut(s) 1095
BseLI CCNNNNNNNGG 2 cut(s) 217, 881
BseMI GCAATG 2 cut(s) 1008, 1114
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 2 cut(s) 601, 799
BseXI GCAGC 1 cut(s) 32
BseYI CCCAGC 1 cut(s) 288
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 3 cut(s) 323, 348, 886
BshNI GGYRCC 2 cut(s) 153, 475
BsiEI CGRYCG 1 cut(s) 304
BsiHKCI CYCGRG 1 cut(s) 1079
BsiSI CCGG 4 cut(s) 198, 383, 882, 1219
BslFI GGGAC 3 cut(s) 527, 832, 1214
BslI CCNNNNNNNGG 2 cut(s) 217, 881
BsmAI GTCTC 3 cut(s) 197, 300, 1009
BsmBI CGTCTC 1 cut(s) 300
BsmFI GGGAC 3 cut(s) 527, 832, 1214
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 3 cut(s) 323, 348, 886
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 1079
Bsp143I GATC 3 cut(s) 391, 1039, 1058
BspACI CCGC 7 cut(s) 43, 177, 282, 331, 482, 831, 1022
BspANI GGCC 3 cut(s) 323, 348, 886
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 155, 477
BspPI GGATC 1 cut(s) 1066
BspQI GCTCTTC 1 cut(s) 969
BspT107I GGYRCC 2 cut(s) 153, 475
BspTNI GGTCTC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 282
BsrDI GCAATG 2 cut(s) 1008, 1114
BsrFI RCCGGY 3 cut(s) 197, 382, 1218
BsrI ACTGG 2 cut(s) 601, 799
BssAI RCCGGY 3 cut(s) 197, 382, 1218
BssECI CCNNGG 3 cut(s) 149, 555, 936
BssMI GATC 3 cut(s) 391, 1039, 1058
BssNI GRCGYC 3 cut(s) 101, 154, 312
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 151, 937
Bst4CI ACNGT 5 cut(s) 220, 518, 658, 823, 1205
Bst6I CTCTTC 2 cut(s) 18, 969
BstACI GRCGYC 3 cut(s) 101, 154, 312
BstC8I GCNNGC 2 cut(s) 346, 1047
BstDEI CTNAG 1 cut(s) 189
BstEII GGTNACC 2 cut(s) 193, 599
BstF5I GGATG 1 cut(s) 1095
BstH2I RGCGCY 2 cut(s) 87, 157
BstHHI GCGC 2 cut(s) 86, 156
BstKTI GATC 3 cut(s) 394, 1042, 1061
BstMAI GTCTC 3 cut(s) 197, 300, 1009
BstMBI GATC 3 cut(s) 391, 1039, 1058
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 5 cut(s) 42, 151, 320, 787, 1019
BstNI CCWGG 2 cut(s) 151, 937
BstNSI RCATGY 1 cut(s) 565
BstPI GGTNACC 2 cut(s) 193, 599
BstSCI CCNGG 3 cut(s) 149, 881, 935
BstSFI CTRYAG 2 cut(s) 689, 968
BstV1I GCAGC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 3 cut(s) 323, 348, 886
BtgZI GCGATG 1 cut(s) 970
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 1 cut(s) 1095
BtsIMutI CAGTG 1 cut(s) 792
Cac8I GCNNGC 2 cut(s) 346, 1047
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 2 cut(s) 86, 156
Cfr10I RCCGGY 3 cut(s) 197, 382, 1218
Cfr13I GGNCC 3 cut(s) 380, 733, 884
CseI GACGC 5 cut(s) 109, 320, 350, 419, 746
Csp6I GTAC 3 cut(s) 257, 442, 916
CviAII CATG 3 cut(s) 325, 364, 562
CviQI GTAC 3 cut(s) 257, 442, 916
DdeI CTNAG 1 cut(s) 189
DinI GGCGCC 1 cut(s) 155
DpnI GATC 3 cut(s) 393, 1041, 1060
DpnII GATC 3 cut(s) 391, 1039, 1058
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
Eam1104I CTCTTC 2 cut(s) 18, 969
EarI CTCTTC 2 cut(s) 18, 969
Eco130I CCWWGG 1 cut(s) 555
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 2 cut(s) 380, 733
Eco57I CTGAAG 2 cut(s) 408, 551
Eco88I CYCGRG 1 cut(s) 1079
Eco91I GGTNACC 2 cut(s) 193, 599
EcoO65I GGTNACC 2 cut(s) 193, 599
EcoRII CCWGG 2 cut(s) 149, 935
EcoT14I CCWWGG 1 cut(s) 555
EgeI GGCGCC 1 cut(s) 155
EheI GGCGCC 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 300
FaeI CATG 3 cut(s) 328, 367, 565
FaqI GGGAC 3 cut(s) 527, 832, 1214
FatI CATG 3 cut(s) 324, 363, 561
FauNDI CATATG 1 cut(s) 248
Fnu4HI GCNGC 2 cut(s) 46, 178
FokI GGATG 1 cut(s) 1082
Fsp4HI GCNGC 2 cut(s) 46, 178
FspBI CTAG 3 cut(s) 89, 701, 1037
GlaI GCGC 2 cut(s) 85, 155
GluI GCNGC 2 cut(s) 46, 178
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 816
HaeII RGCGCY 2 cut(s) 87, 157
HaeIII GGCC 3 cut(s) 323, 348, 886
HapII CCGG 4 cut(s) 198, 383, 882, 1219
HgaI GACGC 5 cut(s) 109, 320, 350, 419, 746
HhaI GCGC 2 cut(s) 86, 156
Hin1I GRCGYC 3 cut(s) 101, 154, 312
Hin1II CATG 3 cut(s) 328, 367, 565
Hin6I GCGC 2 cut(s) 84, 154
HinP1I GCGC 2 cut(s) 84, 154
HinfI GANTC 9 cut(s) 213, 236, 297, 712, 798, 949, 1002, 1077, 1180
HpaII CCGG 4 cut(s) 198, 383, 882, 1219
HphI GGTGA 3 cut(s) 187, 232, 1036
Hpy166II GTNNAC 2 cut(s) 434, 1147
Hpy188I TCNGA 3 cut(s) 458, 844, 1090
Hpy188III TCNNGA 5 cut(s) 242, 425, 531, 1037, 1130
Hpy8I GTNNAC 2 cut(s) 434, 1147
Hpy99I CGWCG 3 cut(s) 103, 314, 320
HpyAV CCTTC 2 cut(s) 367, 635
HpyCH4III ACNGT 5 cut(s) 220, 518, 658, 823, 1205
HpyCH4IV ACGT 3 cut(s) 546, 812, 1032
HpyCH4V TGCA 4 cut(s) 590, 611, 1055, 1107
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 151, 320, 787, 1019
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 3 cut(s) 546, 812, 1032
Hsp92I GRCGYC 3 cut(s) 101, 154, 312
Hsp92II CATG 3 cut(s) 328, 367, 565
HspAI GCGC 2 cut(s) 84, 154
KasI GGCGCC 1 cut(s) 153
Kzo9I GATC 3 cut(s) 391, 1039, 1058
LguI GCTCTTC 1 cut(s) 969
LmnI GCTCC 2 cut(s) 333, 427
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 4 cut(s) 411, 598, 922, 993
MaeI CTAG 3 cut(s) 89, 701, 1037
MaeII ACGT 3 cut(s) 546, 812, 1032
MaeIII GTNAC 5 cut(s) 50, 193, 214, 599, 1162
MalI GATC 3 cut(s) 393, 1041, 1060
MbiI CCGCTC 1 cut(s) 282
MboI GATC 3 cut(s) 391, 1039, 1058
MboII GAAGA 7 cut(s) 5, 235, 401, 650, 956, 1151, 1189
MfeI CAATTG 1 cut(s) 26
MflI RGATCY 1 cut(s) 391
MluCI AATT 3 cut(s) 26, 648, 670
Mly113I GGCGCC 1 cut(s) 154
MlyI GAGTC 5 cut(s) 222, 807, 958, 1011, 1071
MmeI TCCRAC 2 cut(s) 926, 1068
MseI TTAA 5 cut(s) 489, 522, 651, 893, 1188
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 4 cut(s) 198, 383, 882, 1219
MspR9I CCNGG 3 cut(s) 151, 883, 937
MunI CAATTG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 2 cut(s) 151, 937
MwoI GCNNNNNNNGC 5 cut(s) 42, 151, 320, 787, 1019
NarI GGCGCC 1 cut(s) 154
NciI CCSGG 1 cut(s) 883
NdeI CATATG 1 cut(s) 248
NdeII GATC 3 cut(s) 391, 1039, 1058
NlaIII CATG 3 cut(s) 328, 367, 565
NlaIV GGNNCC 2 cut(s) 155, 477
NmeAIII GCCGAG 3 cut(s) 115, 182, 278
NmuCI GTSAC 3 cut(s) 193, 214, 1162
NspI RCATGY 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 969
PcsI WCGNNNNNNNCGW 3 cut(s) 306, 315, 427
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 4 cut(s) 236, 297, 712, 1180
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 2 cut(s) 47, 179
PleI GAGTC 5 cut(s) 221, 806, 957, 1010, 1071
PluTI GGCGCC 1 cut(s) 157
PpsI GAGTC 5 cut(s) 221, 806, 957, 1010, 1071
PshBI ATTAAT 1 cut(s) 522
PsiI TTATAA 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 149, 935
PspEI GGTNACC 2 cut(s) 193, 599
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 2 cut(s) 149, 935
PspN4I GGNNCC 2 cut(s) 155, 477
PspPI GGNCC 3 cut(s) 380, 733, 884
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 391
PsyI GACNNNGTC 1 cut(s) 314
RsaI GTAC 3 cut(s) 258, 443, 917
RsaNI GTAC 3 cut(s) 257, 442, 916
RseI CAYNNNNRTG 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 969
SaqAI TTAA 5 cut(s) 489, 522, 651, 893, 1188
SatI GCNGC 2 cut(s) 46, 178
Sau3AI GATC 3 cut(s) 391, 1039, 1058
Sau96I GGNCC 3 cut(s) 380, 733, 884
ScaI AGTACT 2 cut(s) 258, 443
SchI GAGTC 5 cut(s) 222, 807, 958, 1011, 1071
ScrFI CCNGG 3 cut(s) 151, 883, 937
SfaNI GCATC 4 cut(s) 411, 598, 922, 993
SfcI CTRYAG 2 cut(s) 689, 968
SfoI GGCGCC 1 cut(s) 155
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 2 cut(s) 380, 733
SmiMI CAYNNNNRTG 1 cut(s) 59
Sse9I AATT 3 cut(s) 26, 648, 670
SsiI CCGC 7 cut(s) 43, 177, 282, 331, 482, 831, 1022
SspDI GGCGCC 1 cut(s) 153
SspMI CTAG 3 cut(s) 89, 701, 1037
StyD4I CCNGG 3 cut(s) 149, 881, 935
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 5 cut(s) 220, 518, 658, 823, 1205
TaiI ACGT 3 cut(s) 549, 815, 1035
TaqI TCGA 8 cut(s) 300, 318, 360, 415, 675, 750, 855, 1131
TaqII GACCGA 2 cut(s) 157, 318
TasI AATT 3 cut(s) 26, 648, 670
TatI WGTACW 3 cut(s) 256, 441, 915
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 4 cut(s) 236, 297, 712, 1180
Tru1I TTAA 5 cut(s) 489, 522, 651, 893, 1188
Tru9I TTAA 5 cut(s) 489, 522, 651, 893, 1188
TscAI CASTG 1 cut(s) 799
TseFI GTSAC 3 cut(s) 193, 214, 1162
TseI GCWGC 1 cut(s) 45
Tsp45I GTSAC 3 cut(s) 193, 214, 1162
TspDTI ATGAA 2 cut(s) 563, 1227
TspGWI ACGGA 2 cut(s) 279, 1014
TspRI CASTG 1 cut(s) 799
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 380, 733
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 1 cut(s) 670
XbaI TCTAGA 1 cut(s) 1036
XceI RCATGY 1 cut(s) 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XspI CTAG 3 cut(s) 89, 701, 1037
ZrmI AGTACT 2 cut(s) 258, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.