MD12G1009000.v1.1

Exosome component 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004155
Physical Location & Seq
Forward (+)
951922 .. 958736
6815 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009000.v1.1.491

Sequence Viewer

Length: 2787 bp
ATGAGCCAAGACGCGATGACAGTGAACCAATCTCCGCCGCTGCAGTCGAGGTCCGACGCCCTAGAAAACCTAACCAAAGCCCCTCTCTCCTCGTCGATTTCCAAGCTCTCCGGCTCCTCGCGATTCTTGCCGAACAGCAAGGACTTCTATTTCTACCGCAACTTCGACGAATTCAAAGCCCCGATCGAGAAAATCACCAAGGAGACGCAGACGATGCTCGGCTCGATTGGCTCGTCGACGCCCTTTTGGGGGAAACCGATGGCTTTCCCCCAAGACTTGGACGACGCGTACGATTGGCTCGTTAATGTGAACGACGAGGTGCTGGAGAGGTTTGACTCGTCGGTGGACGAGTTCAAGAGGGTGAGGAAGGAGGCGGAGGAGGCCAAGCGGCCGACGAGTGCGGCATTGGACACAGACGATGGGTTTCAGTTGGTGTGTGGGAAGAAGAAGAAAAAGGGGCCCACTGGGTTGGCTTCGGCCAGTGCGAACAATGATTCGAGTCAGGTTTCCACCGCCGTGAAGGTGGCTGCCAAGGACAAGAAAACTGTGGCGGCGACGAAGCCCAAGGTTCCGTTTCATATACCAAGCATTCGGAGGTCTCAGGAGGAGTTTAACATTTTGGTTAACAATGAAAACCAGCCGTTTGCGCATGTTTGGTTGCAGAGAAGTGAGGATGGTCAGCGGTTCCTTCATCCCCTAAAGCTTTCTGTTCTGGATTTTGTTGACAACAACATCGGGGATGTTGAGTCTGTAAAACCGCCTTCATTAAAGTCTACGCCATTCAAGCTTGTGGAGGAGGTCAAAGATCTGAAAAAGCTAGCAGCTAAGCTCCGTGCTGTTAATGAATTTGCGGTTGATTTGGAACACAATCAATATCGATCGTTTCAAGGATTGACTTGCTTGATGCAAATTTCAACTAGAACCGAAGATTTTATAGTAGATACTCTGAAGCTTAGAACTCATGTTGGGCCATATCTTAGGGAAGTTTTCAAGGACCCTGCCAAGAGAAAGGTGATGCATGGAGCAGATCGTGATATCAGTTGGCTCCAACGTGATTTTGGCATTTATATCTGCAATTTGTTTGATACGGGCCAGGCTTCGAGGGTGTTGAAATTGGAAAGAAATAGTTTGGAATATCTTCTACAGCACTTTTGTGGTGTTACTGCTAACAAAGAATACCAGAATGCAGACTGGAGATTACGCCCTCTGCCTGAGGAGATGGTCAGATATGCCAGAGAAGATACACACTATTTGCTGCATATGTATGATTTAATGAGAACACAGTTATGTCTAATGCCCAAGGAATCAGAAAACTCTGACACTCCTTTAGTAGAGATGGATTATTGTCTGAATTGGTGTTTTACTGTTGAATATGTCTTGATGGTTTACAAACGCAGTTATGATATATGCATGCATCTGTATGAGAAAGATCTTTTGACAGAAGATTCTTATCTCCACATATACGGGTTGCAAGGTGCTGGTTTCAATTCTCAGCAGCTTGCCATTGTTTCCGGTCTTTGTGAGTGGAGGGACGTTGTTGCCCGTGCAGAGGATGAAAGCACTGGCTATATATTACCAAATAAAACTCTTCTTGAAATTGCTAAACAGATGCCTGTCACCACTAGCAAGTTAAGGCGGTTGGTGAAATCAAAGCATCCATACATTGAGCGTAATCTTGGGTCAGTTGTTAGTATCATTAGACATTCTATGCAAAATGCTGCTTCTTTTGAGCCTGCTGTTGAACATCTGGCGGCACGCGCAAGAATGGCATCTGAAGAGAGAATATTGGTCAATGATGGGTCTGAAGCCTTGTTACCGGATCAATCTGTCAGCAATTCGACTAATGGTGATGTATCTGCAGTTTCACCCCCGTCTAAGCAGCATAAAATGGAGTATAAGGAGATTGCATTTGGTGCCTCTGAACTTGTAAGAAATGGACAAGGAAACTCTCTTGAAGGCAGCCCCATTTCAGTAAATCTTAGGCAAAACAGTGTTCCGGGGCAAAACAGAGAAGCAAGCAGCATTGCCTGTTTATTAGATTCAGCAAAGGTTACTGGAGTTAGTGTTCAGGTGCAAAAGAAGCCTAGCCGTGCCTTTAGTTCGCTGTTGGGGAGTTCAGTTCCAAAAAGAAAGTTTGACGCTGATAAAAAGGACAAGGAAGGTTATAAGTTGGAGCAAATTAGATCTTCAGTGAACTTCCCATTCCGCTCATTTTCGGGCGGGAGTGAGAAAACCAAACCAATCGTGGAAGCACAAGATAAAAGTTCTGATACTCCCCATTCTGAAGGGCCGCTTACTGCATCACCCCCTGGGTCTGGTTTAGATGACATCATAACGTTGGAAAATGACTCAGATGGAGGTGAGCCAGTAGATGGTTCCTCAGAAACCAGAAATGAGCCCGAGGAAAATGATTCAGTACCATCAGCTTTGGAAGGGGATAGGGAAGACGAACCTGTGTCTCTCTCTGGTTTGTCCTCAAGCTTCCAAAATTGCTTTGAGTCGCTTAACCAAAACAGGAAGACAAGAGAAGTTGAGAAGTCCCTGGAATCTGGTGGTTTGCAGGTGAAGCCTTTCGACTATGAGGCAGCAAAGGGTCAGGTCATATTTGGAGCTAAACCGATCAGGGAAGCTGGAGCTGCGGAAGGTGTAAAGAGCTTAAATTCAGCGGGAAAGAAAAAAGCTTTAGCCGGTCGAGTTTCAAACGATGATGGGTCAAAAGAACTAGGACAGGGTAGACGACGCCAAGCTTTTCCGGCGACTGGAAATAGAAGTGCGACATTTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016458 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031047 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032259 GO:0032268 GO:0032270 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0045934 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051641 GO:0060255 GO:0061085 GO:0061087 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902275 GO:1902464 GO:1902466 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905269 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC PMC2NT

Protein Analysis

929

Amino Acids

103.32

Weight (kDa)

6.24

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMC2NT PF08066 39 - 119 2.6e-07 PMC2NT (NUC016) domain
DNA_pol_A_exo1 PF01612 264 - 429 1.2e-47 3'-5' exonuclease
HRDC PF00570 499 - 565 1.8e-13 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2166
AarI CACCTGC 1 cut(s) 2551
Acc16I TGCGCA 1 cut(s) 648
Acc36I ACCTGC 1 cut(s) 2551
AccB1I GGYRCC 1 cut(s) 1913
AccB7I CCANNNNNTGG 2 cut(s) 277, 2372
AccBSI CCGCTC 1 cut(s) 2208
AccI GTMKAC 3 cut(s) 236, 773, 2734
AccII CGCG 4 cut(s) 14, 121, 287, 1758
AclI AACGTT 1 cut(s) 2336
AclWI GGATC 1 cut(s) 1827
AcoI YGGCCR 2 cut(s) 389, 477
AcsI RAATTY 4 cut(s) 170, 845, 909, 2659
AcuI CTGAAG 5 cut(s) 968, 1794, 1824, 2172, 2304
AcyI GRCGYC 3 cut(s) 57, 239, 2740
AfaI GTAC 2 cut(s) 290, 2418
AfiI CCNNNNNNNGG 7 cut(s) 248, 249, 277, 1549, 2315, 2372, 2759
AflIII ACRYGT 1 cut(s) 285
AjnI CCWGG 3 cut(s) 1092, 2308, 2541
AleI CACNNNNGTG 2 cut(s) 515, 1152
AloI GAACNNNNNNTCC 2 cut(s) 2049, 2081
Alw26I GTCTC 3 cut(s) 197, 603, 2463
AlwI GGATC 1 cut(s) 1827
Ama87I CYCGRG 1 cut(s) 2399
AoxI GGCC 7 cut(s) 381, 389, 458, 477, 968, 1090, 2288
ApaI GGGCCC 1 cut(s) 462
ApoI RAATTY 4 cut(s) 170, 845, 909, 2659
Asp700I GAANNNNTTC 2 cut(s) 1137, 2570
AspLEI GCGC 2 cut(s) 649, 1760
AspS9I GGNCC 7 cut(s) 51, 458, 459, 968, 994, 1090, 2288
AsuC2I CCSGG 1 cut(s) 1998
AsuNHI GCTAGC 1 cut(s) 817
AvaI CYCGRG 1 cut(s) 2399
AvaII GGWCC 2 cut(s) 51, 994
AxyI CCTNAGG 1 cut(s) 1212
BaeGI GKGCMC 1 cut(s) 462
BanI GGYRCC 1 cut(s) 1913
BanII GRGCYC 2 cut(s) 462, 2400
BarI GAAGNNNNNNTAC 2 cut(s) 1797, 1829
BbsI GAAGAC 2 cut(s) 2451, 2525
BceAI ACGGC 3 cut(s) 500, 625, 2073
BciT130I CCWGG 3 cut(s) 1094, 2310, 2543
BcnI CCSGG 1 cut(s) 1998
BcoDI GTCTC 3 cut(s) 197, 603, 2463
BfaI CTAG 6 cut(s) 62, 818, 918, 1623, 2085, 2723
BfmI CTRYAG 3 cut(s) 41, 1142, 1857
BfuAI ACCTGC 1 cut(s) 2551
BglII AGATCT 3 cut(s) 805, 1429, 2183
BlpI GCTNAGC 1 cut(s) 825
Bme1390I CCNGG 4 cut(s) 1094, 1998, 2310, 2543
Bme18I GGWCC 2 cut(s) 51, 994
BmeT110I CYCGRG 1 cut(s) 2399
BmgT120I GGNCC 7 cut(s) 51, 458, 459, 968, 994, 1090, 2288
BmiI GGNNCC 9 cut(s) 115, 459, 460, 570, 686, 996, 1046, 1915, 2377
BmrFI CCNGG 4 cut(s) 1094, 1998, 2310, 2543
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 8 cut(s) 204, 894, 1005, 1423, 1599, 1663, 1778, 2309
BmtI GCTAGC 1 cut(s) 821
BmuI ACTGGG 1 cut(s) 474
BpiI GAAGAC 2 cut(s) 2451, 2525
BpmI CTGGAG 4 cut(s) 344, 1213, 2076, 2652
Bpu1102I GCTNAGC 1 cut(s) 825
BpuEI CTTGAG 1 cut(s) 2461
BpuMI CCSGG 1 cut(s) 1998
Bsa29I ATCGAT 1 cut(s) 877
BsaBI GATNNNNATC 1 cut(s) 1449
BsaHI GRCGYC 3 cut(s) 57, 239, 2740
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 9 cut(s) 198, 531, 564, 1299, 1997, 2308, 2309, 2400, 2541
BsaWI WCCGGW 2 cut(s) 1511, 1816
BsaXI ACNNNNNCTCC 2 cut(s) 2049, 2079
Bsc4I CCNNNNNNNGG 7 cut(s) 248, 249, 277, 1549, 2315, 2372, 2759
Bse118I RCCGGY 1 cut(s) 2687
Bse1I ACTGG 7 cut(s) 469, 480, 1196, 1567, 2059, 2366, 2764
Bse21I CCTNAGG 1 cut(s) 1212
Bse3DI GCAATG 1 cut(s) 2022
Bse8I GATNNNNATC 1 cut(s) 1449
BseBI CCWGG 3 cut(s) 1094, 2310, 2543
BseCI ATCGAT 1 cut(s) 877
BseDI CCNNGG 9 cut(s) 198, 531, 564, 1299, 1997, 2308, 2309, 2400, 2541
BseGI GGATG 5 cut(s) 679, 691, 745, 1558, 1654
BseJI GATNNNNATC 1 cut(s) 1449
BseLI CCNNNNNNNGG 7 cut(s) 248, 249, 277, 1549, 2315, 2372, 2759
BseMI GCAATG 1 cut(s) 2022
BseMII CTCAG 5 cut(s) 614, 1203, 1505, 2364, 2394
BseNI ACTGG 7 cut(s) 469, 480, 1196, 1567, 2059, 2366, 2764
BseRI GAGGAG 6 cut(s) 79, 106, 392, 620, 809, 1229
BseSI GKGCMC 1 cut(s) 462
BseX3I CGGCCG 1 cut(s) 389
BsgI GTGCAG 1 cut(s) 1566
Bsh1236I CGCG 4 cut(s) 14, 121, 287, 1758
Bsh1285I CGRYCG 4 cut(s) 186, 392, 881, 2692
BshFI GGCC 7 cut(s) 383, 391, 460, 479, 970, 1092, 2290
BshNI GGYRCC 1 cut(s) 1913
BshVI ATCGAT 1 cut(s) 877
BsiEI CGRYCG 4 cut(s) 186, 392, 881, 2692
BsiHKCI CYCGRG 1 cut(s) 2399
BsiSI CCGG 6 cut(s) 111, 1512, 1817, 1997, 2688, 2753
BsiWI CGTACG 1 cut(s) 288
BslFI GGGAC 2 cut(s) 1544, 2524
BslI CCNNNNNNNGG 7 cut(s) 248, 249, 277, 1549, 2315, 2372, 2759
BsmAI GTCTC 3 cut(s) 197, 603, 2463
BsmBI CGTCTC 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 1544, 2524
BsmI GAATGC 2 cut(s) 588, 1189
BsnI GGCC 7 cut(s) 383, 391, 460, 479, 970, 1092, 2290
Bso31I GGTCTC 1 cut(s) 603
BsoBI CYCGRG 1 cut(s) 2399
Bsp120I GGGCCC 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 462, 2400
Bsp143I GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
Bsp1720I GCTNAGC 1 cut(s) 825
Bsp68I TCGCGA 1 cut(s) 121
BspANI GGCC 7 cut(s) 383, 391, 460, 479, 970, 1092, 2290
BspCNI CTCAG 5 cut(s) 613, 1204, 1504, 2363, 2393
BspDI ATCGAT 1 cut(s) 877
BspFNI CGCG 4 cut(s) 14, 121, 287, 1758
BspLI GGNNCC 9 cut(s) 115, 459, 460, 570, 686, 996, 1046, 1915, 2377
BspMAI CTGCAG 2 cut(s) 45, 1861
BspMI ACCTGC 1 cut(s) 2551
BspOI GCTAGC 1 cut(s) 821
BspPI GGATC 1 cut(s) 1827
BspT107I GGYRCC 1 cut(s) 1913
BspTNI GGTCTC 1 cut(s) 603
BsrBI CCGCTC 1 cut(s) 2208
BsrDI GCAATG 1 cut(s) 2022
BsrFI RCCGGY 1 cut(s) 2687
BsrI ACTGG 7 cut(s) 469, 480, 1196, 1567, 2059, 2366, 2764
BssAI RCCGGY 1 cut(s) 2687
BssECI CCNNGG 9 cut(s) 198, 531, 564, 1299, 1997, 2308, 2309, 2400, 2541
BssMI GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
BssNI GRCGYC 3 cut(s) 57, 239, 2740
BssT1I CCWWGG 4 cut(s) 198, 531, 564, 1299
Bst2UI CCWGG 3 cut(s) 1094, 2310, 2543
Bst4CI ACNGT 5 cut(s) 22, 547, 1284, 1366, 1991
Bst6I CTCTTC 2 cut(s) 1593, 1770
BstACI GRCGYC 3 cut(s) 57, 239, 2740
BstAPI GCANNNNNTGC 2 cut(s) 214, 1913
BstC8I GCNNGC 6 cut(s) 819, 1412, 1500, 1734, 1756, 2017
BstF5I GGATG 5 cut(s) 679, 691, 745, 1558, 1654
BstFNI CGCG 4 cut(s) 14, 121, 287, 1758
BstHHI GCGC 2 cut(s) 649, 1760
BstKTI GATC 8 cut(s) 186, 808, 881, 1030, 1432, 1822, 2186, 2622
BstMAI GTCTC 3 cut(s) 197, 603, 2463
BstMBI GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
BstMCI CGRYCG 4 cut(s) 186, 392, 881, 2692
BstNI CCWGG 3 cut(s) 1094, 2310, 2543
BstNSI RCATGY 2 cut(s) 653, 1414
BstSCI CCNGG 4 cut(s) 1092, 1996, 2308, 2541
BstSFI CTRYAG 3 cut(s) 41, 1142, 1857
BstSLI GKGCMC 1 cut(s) 462
BstUI CGCG 4 cut(s) 14, 121, 287, 1758
BstV2I GAAGAC 2 cut(s) 2451, 2525
BstX2I RGATCY 3 cut(s) 805, 1429, 2183
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 3 cut(s) 805, 1429, 2183
BstZI CGGCCG 1 cut(s) 389
Bsu15I ATCGAT 1 cut(s) 877
Bsu36I CCTNAGG 1 cut(s) 1212
BsuRI GGCC 7 cut(s) 383, 391, 460, 479, 970, 1092, 2290
BsuTUI ATCGAT 1 cut(s) 877
BtgZI GCGATG 1 cut(s) 29
BtsCI GGATG 5 cut(s) 679, 691, 745, 1558, 1654
BtsIMutI CAGTG 6 cut(s) 27, 462, 487, 1560, 1996, 2196
BtuMI TCGCGA 1 cut(s) 121
BveI ACCTGC 1 cut(s) 2551
Cac8I GCNNGC 6 cut(s) 819, 1412, 1500, 1734, 1756, 2017
CfoI GCGC 2 cut(s) 649, 1760
Cfr10I RCCGGY 1 cut(s) 2687
Cfr13I GGNCC 7 cut(s) 51, 458, 459, 968, 994, 1090, 2288
ClaI ATCGAT 1 cut(s) 877
CseI GACGC 7 cut(s) 20, 65, 214, 247, 293, 2147, 2748
Csp6I GTAC 2 cut(s) 289, 2417
CviAII CATG 4 cut(s) 650, 962, 1019, 1411
CviQI GTAC 2 cut(s) 289, 2417
DpnI GATC 8 cut(s) 185, 807, 880, 1029, 1431, 1821, 2185, 2621
DpnII GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
EaeI YGGCCR 2 cut(s) 389, 477
EagI CGGCCG 1 cut(s) 389
Eam1104I CTCTTC 2 cut(s) 1593, 1770
EarI CTCTTC 2 cut(s) 1593, 1770
EciI GGCGGA 2 cut(s) 24, 389
EclXI CGGCCG 1 cut(s) 389
Eco130I CCWWGG 4 cut(s) 198, 531, 564, 1299
Eco24I GRGCYC 2 cut(s) 462, 2400
Eco31I GGTCTC 1 cut(s) 603
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 2 cut(s) 51, 994
Eco52I CGGCCG 1 cut(s) 389
Eco57I CTGAAG 5 cut(s) 968, 1794, 1824, 2172, 2304
Eco81I CCTNAGG 1 cut(s) 1212
Eco88I CYCGRG 1 cut(s) 2399
EcoO109I RGGNCCY 2 cut(s) 458, 994
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 3 cut(s) 1092, 2308, 2541
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 4 cut(s) 198, 531, 564, 1299
EcoT22I ATGCAT 3 cut(s) 1020, 1412, 1416
EcoT38I GRGCYC 2 cut(s) 462, 2400
ErhI CCWWGG 4 cut(s) 198, 531, 564, 1299
Esp3I CGTCTC 1 cut(s) 197
FaeI CATG 4 cut(s) 653, 965, 1022, 1414
FalI AAGNNNNNCTT 2 cut(s) 2277, 2309
FaqI GGGAC 2 cut(s) 1544, 2524
FatI CATG 4 cut(s) 649, 961, 1018, 1410
FauI CCCGC 2 cut(s) 2213, 2659
FauNDI CATATG 1 cut(s) 1260
FblI GTMKAC 3 cut(s) 236, 773, 2734
FokI GGATG 5 cut(s) 678, 686, 752, 1565, 1641
FriOI GRGCYC 2 cut(s) 462, 2400
FspBI CTAG 6 cut(s) 62, 818, 918, 1623, 2085, 2723
FspI TGCGCA 1 cut(s) 648
GlaI GCGC 2 cut(s) 648, 1759
GsuI CTGGAG 4 cut(s) 344, 1213, 2076, 2652
HaeIII GGCC 7 cut(s) 383, 391, 460, 479, 970, 1092, 2290
HapII CCGG 6 cut(s) 111, 1512, 1817, 1997, 2688, 2753
HgaI GACGC 7 cut(s) 20, 65, 214, 247, 293, 2147, 2748
HhaI GCGC 2 cut(s) 649, 1760
Hin1I GRCGYC 3 cut(s) 57, 239, 2740
Hin1II CATG 4 cut(s) 653, 965, 1022, 1414
Hin6I GCGC 2 cut(s) 647, 1758
HinP1I GCGC 2 cut(s) 647, 1758
HincII GTYRAC 3 cut(s) 237, 625, 724
HindII GTYRAC 3 cut(s) 237, 625, 724
HindIII AAGCTT 6 cut(s) 701, 785, 950, 2479, 2679, 2745
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 6 cut(s) 111, 1512, 1817, 1997, 2688, 2753
Hpy188III TCNNGA 9 cut(s) 120, 187, 355, 602, 713, 1031, 1378, 1592, 1952
HpyAV CCTTC 9 cut(s) 361, 514, 698, 771, 1949, 2153, 2279, 2426, 2636
HpyCH4III ACNGT 5 cut(s) 22, 547, 1284, 1366, 1991
HpyCH4IV ACGT 3 cut(s) 1051, 1533, 2336
HpySE526I ACGT 3 cut(s) 1051, 1533, 2336
Hsp92I GRCGYC 3 cut(s) 57, 239, 2740
Hsp92II CATG 4 cut(s) 653, 965, 1022, 1414
HspAI GCGC 2 cut(s) 647, 1758
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
LmnI GCTCC 7 cut(s) 119, 834, 1022, 1050, 2173, 2609, 2633
LweI GCATC 8 cut(s) 204, 894, 1005, 1423, 1599, 1663, 1778, 2309
MaeI CTAG 6 cut(s) 62, 818, 918, 1623, 2085, 2723
MaeII ACGT 3 cut(s) 1051, 1533, 2336
MaeIII GTNAC 4 cut(s) 1159, 1615, 1812, 2050
MalI GATC 8 cut(s) 185, 807, 880, 1029, 1431, 1821, 2185, 2621
MbiI CCGCTC 1 cut(s) 2208
MboI GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
MflI RGATCY 3 cut(s) 805, 1429, 2183
MhlI GDGCHC 2 cut(s) 462, 2400
MluI ACGCGT 1 cut(s) 285
MlyI GAGTC 5 cut(s) 329, 508, 755, 2342, 2507
MmeI TCCRAC 4 cut(s) 78, 1072, 2151, 2319
Mph1103I ATGCAT 3 cut(s) 1020, 1412, 1416
MroXI GAANNNNTTC 2 cut(s) 1137, 2570
MslI CAYNNNNRTG 5 cut(s) 515, 1152, 1263, 1285, 1419
MspA1I CMGCKG 3 cut(s) 40, 682, 2666
MspI CCGG 6 cut(s) 111, 1512, 1817, 1997, 2688, 2753
MspR9I CCNGG 4 cut(s) 1094, 1998, 2310, 2543
Mva1269I GAATGC 2 cut(s) 588, 1189
MvaI CCWGG 3 cut(s) 1094, 2310, 2543
MvnI CGCG 4 cut(s) 14, 121, 287, 1758
NciI CCSGG 1 cut(s) 1998
NdeI CATATG 1 cut(s) 1260
NdeII GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
NheI GCTAGC 1 cut(s) 817
NlaIII CATG 4 cut(s) 653, 965, 1022, 1414
NlaIV GGNNCC 9 cut(s) 115, 459, 460, 570, 686, 996, 1046, 1915, 2377
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 1615
NruI TCGCGA 1 cut(s) 121
NsbI TGCGCA 1 cut(s) 648
NsiI ATGCAT 3 cut(s) 1020, 1412, 1416
NspI RCATGY 2 cut(s) 653, 1414
OliI CACNNNNGTG 2 cut(s) 515, 1152
PaeI GCATGC 1 cut(s) 1414
PaqCI CACCTGC 1 cut(s) 2551
PasI CCCWGGG 1 cut(s) 2309
PcsI WCGNNNNNNNCGW 4 cut(s) 230, 288, 297, 482
PctI GAATGC 2 cut(s) 588, 1189
PdmI GAANNNNTTC 2 cut(s) 1137, 2570
PfeI GAWTC 7 cut(s) 123, 494, 1304, 1445, 2039, 2411, 2546
Pfl23II CGTACG 1 cut(s) 288
PflMI CCANNNNNTGG 2 cut(s) 277, 2372
Ple19I CGATCG 2 cut(s) 186, 881
PleI GAGTC 5 cut(s) 329, 507, 754, 2342, 2506
PpsI GAGTC 5 cut(s) 329, 507, 754, 2342, 2506
PpuMI RGGWCCY 1 cut(s) 994
PsiI TTATAA 1 cut(s) 2166
Psp1406I AACGTT 1 cut(s) 2336
Psp5II RGGWCCY 1 cut(s) 994
Psp6I CCWGG 3 cut(s) 1092, 2308, 2541
PspGI CCWGG 3 cut(s) 1092, 2308, 2541
PspLI CGTACG 1 cut(s) 288
PspN4I GGNNCC 9 cut(s) 115, 459, 460, 570, 686, 996, 1046, 1915, 2377
PspOMI GGGCCC 1 cut(s) 458
PspPI GGNCC 7 cut(s) 51, 458, 459, 968, 994, 1090, 2288
PspPPI RGGWCCY 1 cut(s) 994
PstI CTGCAG 2 cut(s) 45, 1861
PsuI RGATCY 3 cut(s) 805, 1429, 2183
PvuI CGATCG 2 cut(s) 186, 881
RruI TCGCGA 1 cut(s) 121
RsaI GTAC 2 cut(s) 290, 2418
RsaNI GTAC 2 cut(s) 289, 2417
RseI CAYNNNNRTG 5 cut(s) 515, 1152, 1263, 1285, 1419
SalI GTCGAC 1 cut(s) 235
Sau3AI GATC 8 cut(s) 183, 805, 878, 1027, 1429, 1819, 2183, 2619
Sau96I GGNCC 7 cut(s) 51, 458, 459, 968, 994, 1090, 2288
SchI GAGTC 5 cut(s) 329, 508, 755, 2342, 2507
ScrFI CCNGG 4 cut(s) 1094, 1998, 2310, 2543
SduI GDGCHC 2 cut(s) 462, 2400
SfaNI GCATC 8 cut(s) 204, 894, 1005, 1423, 1599, 1663, 1778, 2309
SfcI CTRYAG 3 cut(s) 41, 1142, 1857
SgrDI CGTCGACG 1 cut(s) 235
SinI GGWCC 2 cut(s) 51, 994
SmiMI CAYNNNNRTG 5 cut(s) 515, 1152, 1263, 1285, 1419
SmlI CTYRAG 1 cut(s) 2476
SmoI CTYRAG 1 cut(s) 2476
SphI GCATGC 1 cut(s) 1414
SspI AATATT 1 cut(s) 1785
SspMI CTAG 6 cut(s) 62, 818, 918, 1623, 2085, 2723
StyD4I CCNGG 4 cut(s) 1092, 1996, 2308, 2541
StyI CCWWGG 4 cut(s) 198, 531, 564, 1299
TaaI ACNGT 5 cut(s) 22, 547, 1284, 1366, 1991
TaiI ACGT 3 cut(s) 1054, 1536, 2339
TauI GCSGC 6 cut(s) 40, 391, 404, 554, 1754, 2293
TfiI GAWTC 7 cut(s) 123, 494, 1304, 1445, 2039, 2411, 2546
TscAI CASTG 6 cut(s) 27, 469, 487, 1567, 1996, 2196
TseFI GTSAC 1 cut(s) 1615
Tsp45I GTSAC 1 cut(s) 1615
TspDTI ATGAA 6 cut(s) 566, 645, 680, 753, 858, 1569
TspGWI ACGGA 2 cut(s) 561, 821
TspRI CASTG 6 cut(s) 27, 469, 487, 1567, 1996, 2196
Van91I CCANNNNNTGG 2 cut(s) 277, 2372
VpaK11BI GGWCC 2 cut(s) 51, 994
XapI RAATTY 4 cut(s) 170, 845, 909, 2659
XceI RCATGY 2 cut(s) 653, 1414
XcmI CCANNNNNNNNNTGG 2 cut(s) 1055, 2242
XmiI GTMKAC 3 cut(s) 236, 773, 2734
XmnI GAANNNNTTC 2 cut(s) 1137, 2570
XspI CTAG 6 cut(s) 62, 818, 918, 1623, 2085, 2723
Zsp2I ATGCAT 3 cut(s) 1020, 1412, 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.