RchiOBHm_Chr4g0394611

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004155
Physical Location & Seq
Reverse (-)
10267694 .. 10268482
789 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36709

Sequence Viewer

Length: 480 bp
ATGGAAAGTCCGCCGATTGAGCTGAAATGTGTGACGGAGAAGAATTTGGATTCGGAAATGAGGGAGGAGTATGTTTGGGTTGAGAATGAGATTCAGTTGAAGGAAATGGTTGAGGTTTTGAGTAAAGAGAAGATCTTTGGGGTTGATACTGAGCAGCATAGCTTAAGGTCATTTTTGGGATTTACTGCTTTGATACAGATTTCGACTGAGAAGGAAGATTATTTAGTGGACACTATTGCTTTACATGACAGTATGAGCCTTTTGCAGCCGGTTTTCGCCGATGCTGGTATCTGCAAGGCGTTCCATGGGGCAGATAGTGATGTTCTCTGGCTGCAAAGGGACTTTCATATTTATGTTGTCAATATGTTTGATACTGCAAAGGCTTGTGAAGTGTTATCAAAACCGCAGAAATCACTGGCTTATTTACTTGAATCTTACTGTGGAGTTGTCACTAACAAATTGTTTCAGGTTAGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045934 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051179 GO:0051641 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC

Protein Analysis

159

Amino Acids

18.2

Weight (kDa)

4.74

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 24 - 154 3.6e-36 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 11, 404
AcsI RAATTY 1 cut(s) 43
AflII CTTAAG 1 cut(s) 163
AgsI TTSAA 2 cut(s) 100, 431
AluBI AGCT 2 cut(s) 22, 162
AluI AGCT 2 cut(s) 22, 162
ApeKI GCWGC 3 cut(s) 154, 265, 331
ApoI RAATTY 1 cut(s) 43
BbvI GCAGC 3 cut(s) 166, 277, 318
BfrI CTTAAG 1 cut(s) 163
BglII AGATCT 1 cut(s) 132
BisI GCNGC 3 cut(s) 155, 266, 332
BlsI GCNGC 3 cut(s) 156, 267, 333
BmsI GCATC 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 304
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 304
BseMII CTCAG 2 cut(s) 141, 198
BseNI ACTGG 1 cut(s) 420
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 3 cut(s) 166, 277, 318
BsiSI CCGG 1 cut(s) 269
BslFI GGGAC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 353
Bsp143I GATC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 2 cut(s) 11, 404
BspCNI CTCAG 2 cut(s) 142, 199
BspTI CTTAAG 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 1 cut(s) 420
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 251, 440
BstAFI CTTAAG 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 150, 207
BstDSI CCRYGG 1 cut(s) 304
BstKTI GATC 1 cut(s) 135
BstMBI GATC 1 cut(s) 132
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstV1I GCAGC 3 cut(s) 166, 277, 318
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BtgI CCRYGG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 413
Cfr10I RCCGGY 1 cut(s) 268
CviAII CATG 2 cut(s) 245, 305
CviJI RGCY 7 cut(s) 22, 162, 258, 268, 331, 383, 419
CviKI_1 RGCY 7 cut(s) 22, 162, 258, 268, 331, 383, 419
DdeI CTNAG 2 cut(s) 150, 207
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 2 cut(s) 248, 308
FaiI YATR 8 cut(s) 72, 159, 246, 254, 306, 348, 354, 365
FaqI GGGAC 1 cut(s) 353
FatI CATG 2 cut(s) 244, 304
Fnu4HI GCNGC 3 cut(s) 155, 266, 332
Fsp4HI GCNGC 3 cut(s) 155, 266, 332
GluI GCNGC 3 cut(s) 155, 266, 332
HapII CCGG 1 cut(s) 269
Hin1II CATG 2 cut(s) 248, 308
HinfI GANTC 3 cut(s) 50, 91, 431
HpaII CCGG 1 cut(s) 269
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 55
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 2 cut(s) 94, 205
HpyCH4III ACNGT 2 cut(s) 251, 440
HpyCH4V TGCA 4 cut(s) 265, 294, 334, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 2 cut(s) 150, 207
Hsp92II CATG 2 cut(s) 248, 308
Kzo9I GATC 1 cut(s) 132
LpnPI CCDG 5 cut(s) 270, 282, 313, 401, 452
Lsp1109I GCAGC 3 cut(s) 166, 277, 318
LweI GCATC 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 31, 448
MalI GATC 1 cut(s) 134
MboI GATC 1 cut(s) 132
MboII GAAGA 3 cut(s) 52, 142, 227
MflI RGATCY 1 cut(s) 132
MluCI AATT 2 cut(s) 43, 458
MnlI CCTC 3 cut(s) 54, 58, 106
MseI TTAA 1 cut(s) 164
MslI CAYNNNNRTG 1 cut(s) 351
MspCI CTTAAG 1 cut(s) 163
MspI CCGG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 19
NcoI CCATGG 1 cut(s) 304
NdeII GATC 1 cut(s) 132
NlaIII CATG 2 cut(s) 248, 308
NmuCI GTSAC 2 cut(s) 31, 448
PfeI GAWTC 3 cut(s) 50, 91, 431
PkrI GCNGC 3 cut(s) 156, 267, 333
PsuI RGATCY 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 3 cut(s) 155, 266, 332
Sau3AI GATC 1 cut(s) 132
SetI ASST 6 cut(s) 24, 117, 164, 170, 471, 478
SfaNI GCATC 1 cut(s) 271
SgeI CNNG 9 cut(s) 257, 281, 297, 307, 317, 340, 396, 428, 440
SmiMI CAYNNNNRTG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
Sse9I AATT 2 cut(s) 43, 458
SsiI CCGC 2 cut(s) 11, 404
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 251, 440
TaqI TCGA 1 cut(s) 203
TasI AATT 2 cut(s) 43, 458
TfiI GAWTC 3 cut(s) 50, 91, 431
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 2 cut(s) 31, 448
TseI GCWGC 3 cut(s) 154, 265, 331
Tsp45I GTSAC 2 cut(s) 31, 448
TspDTI ATGAA 1 cut(s) 335
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 1 cut(s) 420
Vha464I CTTAAG 1 cut(s) 163
XapI RAATTY 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.