pycom14g00500

Exosome component 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004155
Physical Location & Seq
Forward (+)
340792 .. 345967
5176 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00500.1

Sequence Viewer

Length: 1674 bp
ATGAGCCAAGACGCCGTGACCATGGACCAATCTCCGCCGCCGCAGACGAACTCCGACGCCCTCCAAACCCTAACCAAGGGTGCTCTCTCCTCGTCGATTTCCAAGCTCTCCGGCTCCTCGCGATTCTTGCCTAACAGCAAGGACTTCTATTTCTACCGCAACTTCGACGAATTCAGGGTCCCGATCGAGCAAATCACCAAGGAATCGCAGACGATGCTTGGCTCGATTGGCTCGTCGGCGCCGGTTTGGAGGAAACCGGTGGCTTTCCCCGATGACTTGGACGACGCGTACGATTGGCTCGTTAATGTGAACGACGAGGCTCTGGAGAGGTTTGACTCGTCGGTGGACGAGTTCAAGATTGTGAGGAAGGAGGTGGAGGAGGCCAAGCGGCCGACGAGTGCGGCTATGGACACAGACGATGGGTTTCAATTGGTGTGTGGGAAAAAGAAGAAGGGGCCTACTGGGTCAGCAGCGGCCAGTGGGAACGATGATTCGAGTCAGGTATCCTCTGCGGTGAAGGTGGCTTCCAAGGACAAGAAAACTGTGGTCGCGACGAAGCCGAAAGTGCCGTTTCATATACCGAGCATTCGGAGGCCTCAGGAGGAGTTTAACATTTTGGTTAATAATGCAAACCAGCCGTTTGAGCATGTTTGGTTGCAGAGAAGTGAGGACGGTCAACAGTTTCTTCATCCCCTAGAGAAGCTTTCTGTTCTGAATTTTGTTGACAACAACATTGGGGATGTTGAGTCTGTAAAACCGGCTTCATTAGAGTCCACGCCATTCAAGCTTGTGGAGGAGGTCAAAGATCTGAAGGAGCTCGCAGCTAAGCTCCGTGCTGTTAATGAATTTGCGGTTGATTTGGAACACAATCAATATCGATCGATTCAAGGATTGACTTGCTTGATGCAAATTTCAACTAGAACCGAAGATTTTATTGTAGATACTCTGAAGCTTAGAATTCATGTTGGGCCATATCTTAGGGAAGTTTTCAAAGACCCTGCCAAGAGAAAGGTGATGCATGGAGCAGATCGTGATATCATGTGGCTTCAACGTGATTTTGGCATTTATATCTGCAATTTGTTTGATACTGGCCAGGCTTCGAGGGTGTTGAAATTGGAGAGAAATAGTTTGGAGTATCTTCTACAGAACTTATGTGGTGTTACTGCCAACAAAGAATACCAGAATGCAGACTGGAGATTACGCCCTCTGCCTGAGGAGATGATCAGATATGCCAGAGAAGATACACACTATTTATTGCATATGTATGATTTAATGAGAACACAGTTATGTCTGATGCCCAAGGAATCAGAAAACTCTGACACTCCTTTAGTGGAGGTTTACAAACGCAGTTATGATCTATGCATGCATCTATATGAGAAAGAGCTTTTGACAGAAAATTCTTATCTCCACATATACGGGTTGCAAGGTGCTGGTTTCAATTCTCAGCAGCTTGCCATTGTTTCCGGTCTTTGTGAGTGGAGGGATGTTGTTGCCCGTGCAGAGGATGAAAGTACTGGCTATATATTACCAAACAAAACTCTTCTTGAAATTGTTGTTAGTATCATTAGACATTCTATGCAAAATGCTGCTTTTTTTGAGCCTGCTGTCGAACATCTGAAGGTGGCACGTGCAGGAATGCTGAAGAGAATATATTGGTCAATGAGATGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016458 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031047 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032259 GO:0032268 GO:0032270 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0045934 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051641 GO:0060255 GO:0061085 GO:0061087 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902275 GO:1902464 GO:1902466 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905269 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC PMC2NT

Protein Analysis

558

Amino Acids

63.54

Weight (kDa)

5.69

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMC2NT PF08066 39 - 119 2.7e-07 PMC2NT (NUC016) domain
DNA_pol_A_exo1 PF01612 264 - 429 4.4e-47 3'-5' exonuclease
HRDC PF00570 483 - 519 2.5e-07 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccII CGCG 3 cut(s) 121, 287, 551
AciI CCGC 9 cut(s) 35, 38, 41, 157, 388, 401, 473, 512, 851
AcoI YGGCCR 3 cut(s) 389, 474, 1090
AcsI RAATTY 6 cut(s) 170, 715, 845, 909, 957, 1396
AcuI CTGAAG 4 cut(s) 830, 968, 1637, 1661
AcvI CACGTG 1 cut(s) 1628
AcyI GRCGYC 3 cut(s) 12, 57, 239
AfaI GTAC 2 cut(s) 290, 1513
AfiI CCNNNNNNNGG 2 cut(s) 76, 1501
AflIII ACRYGT 1 cut(s) 285
AgeI ACCGGT 1 cut(s) 256
AjnI CCWGG 1 cut(s) 1092
AluBI AGCT 9 cut(s) 106, 703, 787, 817, 824, 829, 952, 1384, 1450
AluI AGCT 9 cut(s) 106, 703, 787, 817, 824, 829, 952, 1384, 1450
Alw21I GWGCWC 2 cut(s) 85, 819
AoxI GGCC 7 cut(s) 381, 389, 455, 474, 593, 968, 1090
ApeKI GCWGC 4 cut(s) 470, 821, 1447, 1586
ApoI RAATTY 6 cut(s) 170, 715, 845, 909, 957, 1396
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AsiGI ACCGGT 1 cut(s) 256
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 4 cut(s) 25, 178, 455, 968
AsuHPI GGTGA 3 cut(s) 187, 526, 1024
AvaII GGWCC 2 cut(s) 25, 178
AxyI CCTNAGG 2 cut(s) 597, 1212
BalI TGGCCA 1 cut(s) 1092
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 819
BbrPI CACGTG 1 cut(s) 1628
Bbv12I GWGCWC 2 cut(s) 85, 819
BbvI GCAGC 4 cut(s) 482, 833, 1459, 1573
BccI CCATC 2 cut(s) 413, 1659
BceAI ACGGC 2 cut(s) 553, 622
BciT130I CCWGG 1 cut(s) 1094
BciVI GTATCC 1 cut(s) 514
BclI TGATCA 1 cut(s) 1221
BfaI CTAG 2 cut(s) 695, 918
BfmI CTRYAG 1 cut(s) 1142
BfoI RGCGCY 1 cut(s) 242
BfuI GTATCC 1 cut(s) 514
BglII AGATCT 1 cut(s) 805
BisI GCNGC 9 cut(s) 38, 41, 389, 402, 471, 474, 822, 1448, 1587
BlpI GCTNAGC 1 cut(s) 825
BlsI GCNGC 9 cut(s) 39, 42, 390, 403, 472, 475, 823, 1449, 1588
BmcAI AGTACT 1 cut(s) 1513
Bme1390I CCNGG 1 cut(s) 1094
Bme18I GGWCC 2 cut(s) 25, 178
BmgT120I GGNCC 4 cut(s) 25, 178, 455, 968
BmiI GGNNCC 5 cut(s) 115, 179, 180, 240, 456
BmrFI CCNGG 1 cut(s) 1094
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 5 cut(s) 204, 894, 1005, 1284, 1375
BmuI ACTGGG 1 cut(s) 471
BpmI CTGGAG 2 cut(s) 344, 1213
Bpu1102I GCTNAGC 1 cut(s) 825
Bsa29I ATCGAT 2 cut(s) 877, 881
BsaAI YACGTR 1 cut(s) 1628
BsaHI GRCGYC 3 cut(s) 12, 57, 239
BsaJI CCNNGG 5 cut(s) 21, 75, 198, 528, 1299
BsaWI WCCGGW 2 cut(s) 256, 1463
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 1501
Bse118I RCCGGY 3 cut(s) 241, 256, 757
Bse1I ACTGG 5 cut(s) 466, 477, 1093, 1196, 1519
Bse21I CCTNAGG 2 cut(s) 597, 1212
BseBI CCWGG 1 cut(s) 1094
BseCI ATCGAT 2 cut(s) 877, 881
BseDI CCNNGG 5 cut(s) 21, 75, 198, 528, 1299
BseGI GGATG 4 cut(s) 688, 745, 1489, 1510
BseLI CCNNNNNNNGG 2 cut(s) 76, 1501
BseMII CTCAG 3 cut(s) 611, 1203, 1457
BseNI ACTGG 5 cut(s) 466, 477, 1093, 1196, 1519
BseRI GAGGAG 6 cut(s) 79, 106, 392, 617, 809, 1229
BseX3I CGGCCG 1 cut(s) 389
BseXI GCAGC 4 cut(s) 482, 833, 1459, 1573
BsgI GTGCAG 2 cut(s) 1518, 1650
Bsh1236I CGCG 3 cut(s) 121, 287, 551
Bsh1285I CGRYCG 3 cut(s) 186, 392, 881
BshFI GGCC 7 cut(s) 383, 391, 457, 476, 595, 970, 1092
BshNI GGYRCC 1 cut(s) 238
BshTI ACCGGT 1 cut(s) 256
BshVI ATCGAT 2 cut(s) 877, 881
BsiEI CGRYCG 3 cut(s) 186, 392, 881
BsiHKAI GWGCWC 2 cut(s) 85, 819
BsiSI CCGG 5 cut(s) 111, 242, 257, 758, 1464
BsiWI CGTACG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 2 cut(s) 76, 1501
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 3 cut(s) 585, 1189, 1641
BsnI GGCC 7 cut(s) 383, 391, 457, 476, 595, 970, 1092
Bsp1286I GDGCHC 2 cut(s) 85, 819
Bsp143I GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
Bsp1720I GCTNAGC 1 cut(s) 825
Bsp19I CCATGG 1 cut(s) 21
Bsp68I TCGCGA 2 cut(s) 121, 551
BspACI CCGC 9 cut(s) 35, 38, 41, 157, 388, 401, 473, 512, 851
BspANI GGCC 7 cut(s) 383, 391, 457, 476, 595, 970, 1092
BspCNI CTCAG 3 cut(s) 610, 1204, 1456
BspDI ATCGAT 2 cut(s) 877, 881
BspFNI CGCG 3 cut(s) 121, 287, 551
BspLI GGNNCC 5 cut(s) 115, 179, 180, 240, 456
BspT107I GGYRCC 1 cut(s) 238
BsrFI RCCGGY 3 cut(s) 241, 256, 757
BsrI ACTGG 5 cut(s) 466, 477, 1093, 1196, 1519
BssAI RCCGGY 3 cut(s) 241, 256, 757
BssECI CCNNGG 5 cut(s) 21, 75, 198, 528, 1299
BssMI GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
BssNI GRCGYC 3 cut(s) 12, 57, 239
BssT1I CCWWGG 5 cut(s) 21, 75, 198, 528, 1299
Bst2UI CCWGG 1 cut(s) 1094
Bst4CI ACNGT 4 cut(s) 544, 674, 681, 1284
Bst6I CTCTTC 2 cut(s) 1545, 1637
BstACI GRCGYC 3 cut(s) 12, 57, 239
BstAPI GCANNNNNTGC 1 cut(s) 214
BstBAI YACGTR 1 cut(s) 1628
BstC8I GCNNGC 4 cut(s) 819, 1364, 1452, 1602
BstDEI CTNAG 6 cut(s) 597, 825, 953, 977, 1212, 1443
BstDSI CCRYGG 1 cut(s) 21
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 4 cut(s) 688, 745, 1489, 1510
BstFNI CGCG 3 cut(s) 121, 287, 551
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 7 cut(s) 186, 808, 881, 1030, 1224, 1357, 1671
BstMBI GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
BstMCI CGRYCG 3 cut(s) 186, 392, 881
BstMWI GCNNNNNNNGC 6 cut(s) 127, 214, 228, 565, 643, 784
BstNI CCWGG 1 cut(s) 1094
BstNSI RCATGY 2 cut(s) 650, 1366
BstSCI CCNGG 1 cut(s) 1092
BstSFI CTRYAG 1 cut(s) 1142
BstUI CGCG 3 cut(s) 121, 287, 551
BstV1I GCAGC 4 cut(s) 482, 833, 1459, 1573
BstX2I RGATCY 2 cut(s) 805, 1668
BstYI RGATCY 2 cut(s) 805, 1668
BstZI CGGCCG 1 cut(s) 389
Bsu15I ATCGAT 2 cut(s) 877, 881
Bsu36I CCTNAGG 2 cut(s) 597, 1212
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 7 cut(s) 383, 391, 457, 476, 595, 970, 1092
BsuTUI ATCGAT 2 cut(s) 877, 881
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 4 cut(s) 688, 745, 1489, 1510
BtsIMutI CAGTG 1 cut(s) 484
BtuMI TCGCGA 2 cut(s) 121, 551
Cac8I GCNNGC 4 cut(s) 819, 1364, 1452, 1602
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 3 cut(s) 241, 256, 757
Cfr13I GGNCC 4 cut(s) 25, 178, 455, 968
ClaI ATCGAT 2 cut(s) 877, 881
CseI GACGC 3 cut(s) 20, 65, 293
Csp6I GTAC 2 cut(s) 289, 1512
CspAI ACCGGT 1 cut(s) 256
CviAII CATG 6 cut(s) 22, 647, 962, 1019, 1039, 1363
CviQI GTAC 2 cut(s) 289, 1512
DdeI CTNAG 6 cut(s) 597, 825, 953, 977, 1212, 1443
DinI GGCGCC 1 cut(s) 240
DpnI GATC 7 cut(s) 185, 807, 880, 1029, 1223, 1356, 1670
DpnII GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
EaeI YGGCCR 3 cut(s) 389, 474, 1090
EagI CGGCCG 1 cut(s) 389
Eam1104I CTCTTC 2 cut(s) 1545, 1637
EarI CTCTTC 2 cut(s) 1545, 1637
EciI GGCGGA 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 817
EclXI CGGCCG 1 cut(s) 389
Eco130I CCWWGG 5 cut(s) 21, 75, 198, 528, 1299
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 819
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 2 cut(s) 25, 178
Eco52I CGGCCG 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 817
Eco57I CTGAAG 4 cut(s) 830, 968, 1637, 1661
Eco72I CACGTG 1 cut(s) 1628
Eco81I CCTNAGG 2 cut(s) 597, 1212
EcoICRI GAGCTC 1 cut(s) 817
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 2 cut(s) 178, 455
EcoRI GAATTC 2 cut(s) 170, 957
EcoRII CCWGG 1 cut(s) 1092
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 5 cut(s) 21, 75, 198, 528, 1299
EcoT22I ATGCAT 3 cut(s) 1020, 1364, 1368
EcoT38I GRGCYC 1 cut(s) 819
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
ErhI CCWWGG 5 cut(s) 21, 75, 198, 528, 1299
FaeI CATG 6 cut(s) 25, 650, 965, 1022, 1042, 1366
FaqI GGGAC 1 cut(s) 164
FatI CATG 6 cut(s) 21, 646, 961, 1018, 1038, 1362
FauNDI CATATG 1 cut(s) 1260
FbaI TGATCA 1 cut(s) 1221
Fnu4HI GCNGC 9 cut(s) 38, 41, 389, 402, 471, 474, 822, 1448, 1587
FokI GGATG 4 cut(s) 675, 752, 1496, 1517
FriOI GRGCYC 1 cut(s) 819
Fsp4HI GCNGC 9 cut(s) 38, 41, 389, 402, 471, 474, 822, 1448, 1587
FspBI CTAG 2 cut(s) 695, 918
GlaI GCGC 1 cut(s) 240
GluI GCNGC 9 cut(s) 38, 41, 389, 402, 471, 474, 822, 1448, 1587
GsuI CTGGAG 2 cut(s) 344, 1213
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 7 cut(s) 383, 391, 457, 476, 595, 970, 1092
HapII CCGG 5 cut(s) 111, 242, 257, 758, 1464
HgaI GACGC 3 cut(s) 20, 65, 293
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 3 cut(s) 12, 57, 239
Hin1II CATG 6 cut(s) 25, 650, 965, 1022, 1042, 1366
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 2 cut(s) 677, 724
HindII GTYRAC 2 cut(s) 677, 724
HindIII AAGCTT 3 cut(s) 701, 785, 950
HinfI GANTC 9 cut(s) 123, 203, 335, 491, 496, 746, 770, 883, 1304
HpaII CCGG 5 cut(s) 111, 242, 257, 758, 1464
HphI GGTGA 3 cut(s) 187, 526, 1024
Hpy166II GTNNAC 6 cut(s) 310, 346, 677, 724, 774, 1339
Hpy188III TCNNGA 8 cut(s) 120, 181, 323, 355, 550, 599, 1031, 1544
Hpy8I GTNNAC 6 cut(s) 310, 346, 677, 724, 774, 1339
Hpy99I CGWCG 9 cut(s) 59, 97, 170, 238, 287, 317, 343, 397, 556
HpyAV CCTTC 5 cut(s) 361, 445, 511, 805, 1612
HpyCH4III ACNGT 4 cut(s) 544, 674, 681, 1284
HpyCH4IV ACGT 2 cut(s) 1051, 1627
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 214, 228, 565, 643, 784
HpyF3I CTNAG 6 cut(s) 597, 825, 953, 977, 1212, 1443
HpySE526I ACGT 2 cut(s) 1051, 1627
Hsp92I GRCGYC 3 cut(s) 12, 57, 239
Hsp92II CATG 6 cut(s) 25, 650, 965, 1022, 1042, 1366
HspAI GCGC 1 cut(s) 239
KasI GGCGCC 1 cut(s) 238
KflI GGGWCCC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 1221
Kzo9I GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
LmnI GCTCC 4 cut(s) 119, 814, 834, 1022
Lsp1109I GCAGC 4 cut(s) 482, 833, 1459, 1573
LweI GCATC 5 cut(s) 204, 894, 1005, 1284, 1375
MaeI CTAG 2 cut(s) 695, 918
MaeII ACGT 2 cut(s) 1051, 1627
MaeIII GTNAC 2 cut(s) 16, 1159
MalI GATC 7 cut(s) 185, 807, 880, 1029, 1223, 1356, 1670
MboI GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
MboII GAAGA 7 cut(s) 460, 677, 938, 1130, 1250, 1532, 1654
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 2 cut(s) 805, 1668
MhlI GDGCHC 2 cut(s) 85, 819
MlsI TGGCCA 1 cut(s) 1092
MluI ACGCGT 1 cut(s) 285
MluNI TGGCCA 1 cut(s) 1092
Mly113I GGCGCC 1 cut(s) 239
MlyI GAGTC 4 cut(s) 329, 505, 755, 779
MmeI TCCRAC 1 cut(s) 78
Mox20I TGGCCA 1 cut(s) 1092
Mph1103I ATGCAT 3 cut(s) 1020, 1364, 1368
MscI TGGCCA 1 cut(s) 1092
MseI TTAA 5 cut(s) 303, 609, 621, 840, 1271
MslI CAYNNNNRTG 3 cut(s) 1263, 1285, 1371
Msp20I TGGCCA 1 cut(s) 1092
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 5 cut(s) 111, 242, 257, 758, 1464
MspR9I CCNGG 1 cut(s) 1094
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 3 cut(s) 585, 1189, 1641
MvaI CCWGG 1 cut(s) 1094
MvnI CGCG 3 cut(s) 121, 287, 551
MwoI GCNNNNNNNGC 6 cut(s) 127, 214, 228, 565, 643, 784
NarI GGCGCC 1 cut(s) 239
NcoI CCATGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 1260
NdeII GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
NlaIII CATG 6 cut(s) 25, 650, 965, 1022, 1042, 1366
NlaIV GGNNCC 5 cut(s) 115, 179, 180, 240, 456
NmuCI GTSAC 1 cut(s) 16
NruI TCGCGA 2 cut(s) 121, 551
NsiI ATGCAT 3 cut(s) 1020, 1364, 1368
NspI RCATGY 2 cut(s) 650, 1366
PaeI GCATGC 1 cut(s) 1366
PceI AGGCCT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 3 cut(s) 230, 288, 297
PctI GAATGC 3 cut(s) 585, 1189, 1641
PfeI GAWTC 5 cut(s) 123, 203, 491, 883, 1304
Pfl23II CGTACG 1 cut(s) 288
PinAI ACCGGT 1 cut(s) 256
PkrI GCNGC 9 cut(s) 39, 42, 390, 403, 472, 475, 823, 1449, 1588
Ple19I CGATCG 2 cut(s) 186, 881
PleI GAGTC 4 cut(s) 329, 504, 754, 778
PluTI GGCGCC 1 cut(s) 242
PmaCI CACGTG 1 cut(s) 1628
PmlI CACGTG 1 cut(s) 1628
PpsI GAGTC 4 cut(s) 329, 504, 754, 778
Ppu21I YACGTR 1 cut(s) 1628
PpuMI RGGWCCY 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 819
Psp5II RGGWCCY 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 1092
PspCI CACGTG 1 cut(s) 1628
PspGI CCWGG 1 cut(s) 1092
PspLI CGTACG 1 cut(s) 288
PspN4I GGNNCC 5 cut(s) 115, 179, 180, 240, 456
PspPI GGNCC 4 cut(s) 25, 178, 455, 968
PspPPI RGGWCCY 1 cut(s) 178
PsuI RGATCY 2 cut(s) 805, 1668
PvuI CGATCG 2 cut(s) 186, 881
RruI TCGCGA 2 cut(s) 121, 551
RsaI GTAC 2 cut(s) 290, 1513
RsaNI GTAC 2 cut(s) 289, 1512
RseI CAYNNNNRTG 3 cut(s) 1263, 1285, 1371
SacI GAGCTC 1 cut(s) 819
SaqAI TTAA 5 cut(s) 303, 609, 621, 840, 1271
SatI GCNGC 9 cut(s) 38, 41, 389, 402, 471, 474, 822, 1448, 1587
Sau3AI GATC 7 cut(s) 183, 805, 878, 1027, 1221, 1354, 1668
Sau96I GGNCC 4 cut(s) 25, 178, 455, 968
ScaI AGTACT 1 cut(s) 1513
SchI GAGTC 4 cut(s) 329, 505, 755, 779
ScrFI CCNGG 1 cut(s) 1094
SduI GDGCHC 2 cut(s) 85, 819
SfaNI GCATC 5 cut(s) 204, 894, 1005, 1284, 1375
SfcI CTRYAG 1 cut(s) 1142
SfoI GGCGCC 1 cut(s) 240
SinI GGWCC 2 cut(s) 25, 178
SmiMI CAYNNNNRTG 3 cut(s) 1263, 1285, 1371
SphI GCATGC 1 cut(s) 1366
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 9 cut(s) 35, 38, 41, 157, 388, 401, 473, 512, 851
SspDI GGCGCC 1 cut(s) 238
SspMI CTAG 2 cut(s) 695, 918
SstI GAGCTC 1 cut(s) 819
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 1 cut(s) 1092
StyI CCWWGG 5 cut(s) 21, 75, 198, 528, 1299
TaaI ACNGT 4 cut(s) 544, 674, 681, 1284
TaiI ACGT 2 cut(s) 1054, 1630
TaqI TCGA 9 cut(s) 95, 165, 186, 224, 494, 877, 881, 1100, 1608
TatI WGTACW 1 cut(s) 1511
TauI GCSGC 5 cut(s) 40, 43, 391, 404, 476
TfiI GAWTC 5 cut(s) 123, 203, 491, 883, 1304
Tru1I TTAA 5 cut(s) 303, 609, 621, 840, 1271
Tru9I TTAA 5 cut(s) 303, 609, 621, 840, 1271
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 4 cut(s) 470, 821, 1447, 1586
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 6 cut(s) 563, 677, 753, 858, 950, 1521
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 25, 178
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 6 cut(s) 170, 715, 845, 909, 957, 1396
XceI RCATGY 2 cut(s) 650, 1366
XspI CTAG 2 cut(s) 695, 918
ZrmI AGTACT 1 cut(s) 1513
Zsp2I ATGCAT 3 cut(s) 1020, 1364, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.