MD14G1006500.v1.1

Exosome component 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004155
Physical Location & Seq
Forward (+)
720146 .. 727197
7052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1006500.v1.1.491

Sequence Viewer

Length: 2745 bp
ATGAGCCATGACGCCGTGACCGTGGACCAATCTCCGCCGCCGCAGACGAAGTCCGACGCCCTCCAAACCCTGACCAAAGGCCCTCTCTCCTCCTCGATTTCCAAGCTCTCCGGCTCCTCGCGATTCTTGCCTAACAGCAAGGACTTCTATTTCTACCGCAACTTCGACGAATTCAGAGTCCCGATCGAGCAAATCACCAAGGAATCGCAGACGATGCTCGGCTCGATTGGCTCGTCGGCGACTGTTTGGGGGAAACCGATGGCTTTCCCCGACGACTTGGACGACGCGTACGATTGGCTCGTTAATGTGAACGACGAGGCTCTGGAGAGGTTTGACTCGTCGGTGGACGAGTTCAAGATTGTGAGGAAGGAGGCGGAGGAGGCCAAGCGGCCGACGAGTGCGGCTATGGACACAGACGATGGGTTTCAATTGGTGTGTGGGAAGAAGAAGAAGGGGTCTACTGGATCGGCAGCCGCCAGTGGAAACGATGATTCAAGTCAGGTCTCCTCTGCGGTGAAGGTGGCTGCCAAGGACAAGAAAACTGCAGTCGCGACGAAGCCGAAAGTGCCATTTCATATACCGAGCATTCGGAGGCCTCAGGAGGAGTTTAACATTTTGGTTAATAATGCAAACCAGCCGTTTGAGCATGTTTGGTTGCAGAGAAGTGAGGACGGTCAACAGTTTCTTCATCCCCTAGAGAAGCTTTCTGTTCTGGATTTTGTTGACAACAACATTGGGGATGTTGAGTCTGTAAAACCGGCTTCATTAGAGTCCACGCCATTCAAGCTTGTGGAGGAGGTCAAAGATCTGAAAGAGCTCGCAGCTAAGCTCCGTGCTGTTAATGAATTTGCGGTCGATTTGGAACACAATCAATATCGATCTTTTCAAGGATTGACTTGCTTGATGCAAATTTCAACTCGAACCGAAGATTTTATAGTAGATACTCTGAAGCTTAGAATTCATGTTGGGCCATATCTTAGGGAAGTTTTCAAAGACCCTGCCAAGAGAAAGGTGATGCATGGAGCAGATCGTGATATCATGTGGCTTCAACGTGATTTTGGCATTTATATCTGCAATTTGTTTGATACTGGCCAGGCTTCGAGGGTGTTGAAATTGGAGAGAAATAGTTTGGAGTATCTTCTACAGAACTTATGTGGTGTTACTGCTAACAAAGAATACCAGAATGCAGACTGGAGATTACGCCCTCTGCCTGAGGAGATGGTCAGATATGCCAGAGAAGATACACACTATTTGTTGCATATGTATGATTTAATGAGAACACAGTTATGTCTGCTGCCCAAGGAATCAGAAAACTCTGACACTCCTTTAGTGGAGGTTTACAAACGCAGTTATGATATATGTATGCATCTGTATGAGAAAGAGCTTTTGACAGAAAATTCTTATCTCCACATATACGGGTTGCATGGTGCGGGTTTCAATTCTCAGCAGCTTGCCATTGTTTCCGGTCTTTGTGAGTGGAGGGATGTTGTTGCTCGTGCAGAGGATGAAAGTACTGGCTATATATTACCAAACAAAACTCTTCTTGAAATTGCTAAACAGATGCCTGTCACCACTAGCAAGTTAAAGCGATTGGTGAAATCAAAGCACCCGTACATTGAGCGTAATCTTGGTTCAGTTGTTAGTATCATTAGACATTCTATGCAAAATGCTGCTTTTTTTGAGCCTGCTGTCGAACATCTGAAGGTGGCACATGCAGGAATGGCATCTGAAGAGAATATATTGGTCAATGATGGATCTGAAGCCTTGTTACCGGATCAGTCTGTCAGCAATTCGACTAATGGTGATGTATCTGTAGTTTCACCCCCGTCTCAGCAGCATGGAATGGAGTATAAGGGGATTGCATTTGGTGCCTCTGAACTTGTAAGAAATGGACAAGGAAACTCTCCTGAAACTGGCAGTCCCATTTCAGTGAATCTTAGGCAGAACAGTGTTCTGGGGCAAAGCAGAGAAGGAAGCAGCAATGCCTGTTTATTAGATTCAGCGAAGGTTACTGGAGTGAGTGTTCAGGTGCAAAAGAAGCCCAGCCGTGCCTTTAGTTCACTGTTGGGGAGTGCAGTTCCAAAAAGGAAGTTCGATGTCGATATAAAGGGCAAGGAAGATAATAAGTTGGAACAAATTAGATCTTCAGTGAACTTCCCATTCCACTCATTTTCGGGCGGGAGTGAGAAATCCAAACCAACCTTGGAAGCACGAGATAAAAGTTCAGAAACTCCCCATTCTGAAGGTCCGCTTACTGCATCACCCTCTGGTTCTGGTTTAGGTGACATCATAACATTGGAAAATGATTCAGATGGAGGTGAACCGGTAGATGGTTCCTCAGAAACCAGAAATGAGCCGGAGGAGAATGATTCGGTACCATCTGCTTGGGGGAGGGATGGGGAAGACGAACCCGTGTCTCTCTCTGATTTGTCGTCGAGCTTCCAAAAATGTTTTCAGTCGCTTAACCAAAACAGGAAGACAAGAGAAGTGGAGAAGTCCCAGGAATCTGGTGGTTTGCAGGTGAAGCCTTTCGACTATGAGGCAGCAAAGAGTCAGGTTATATTCGGAGCTAAGCCGGTCAAAGAGGCTGGAGCTGCGGATGGTGTGAAGAGCTTAAACTCAGCGGGAAAGAAAAAATCTTTAGCTGCTCTAGTTTCAAACGACGATGGGTCAAAAGAACTAGGACAGGGTAGGCGACGCCAAGCTTTTCCAGCAACCGGAAATAGAAGTGCGACATTTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016458 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031047 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032259 GO:0032268 GO:0032270 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0045934 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051641 GO:0060255 GO:0061085 GO:0061087 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902275 GO:1902464 GO:1902466 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905269 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC PMC2NT

Protein Analysis

915

Amino Acids

100.92

Weight (kDa)

5.72

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMC2NT PF08066 39 - 119 2.3e-07 PMC2NT (NUC016) domain
DNA_pol_A_exo1 PF01612 264 - 429 4.4e-47 3'-5' exonuclease
HRDC PF00570 483 - 549 1.8e-13 HRDC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2509
Acc36I ACCTGC 1 cut(s) 2509
Acc65I GGTACC 1 cut(s) 2374
AccB1I GGYRCC 2 cut(s) 1868, 2374
AccI GTMKAC 1 cut(s) 458
AccII CGCG 3 cut(s) 121, 287, 551
AclWI GGATC 3 cut(s) 472, 1762, 1782
AcoI YGGCCR 2 cut(s) 389, 1090
AcsI RAATTY 5 cut(s) 170, 845, 909, 957, 1396
AcuI CTGAAG 6 cut(s) 968, 1721, 1749, 1779, 2130, 2262
AcyI GRCGYC 3 cut(s) 12, 57, 2698
AfaI GTAC 4 cut(s) 290, 1513, 1613, 2376
AfiI CCNNNNNNNGG 3 cut(s) 1913, 2330, 2717
AflIII ACRYGT 1 cut(s) 285
AgeI ACCGGT 1 cut(s) 2323
AhdI GACNNNNNGTC 1 cut(s) 2668
AjnI CCWGG 2 cut(s) 1092, 2499
AjuI GAANNNNNNNTTGG 2 cut(s) 2436, 2468
AloI GAACNNNNNNTCC 2 cut(s) 2007, 2039
Alw21I GWGCWC 1 cut(s) 819
Alw26I GTCTC 3 cut(s) 508, 1833, 2421
AlwI GGATC 3 cut(s) 472, 1762, 1782
AoxI GGCC 6 cut(s) 79, 381, 389, 593, 968, 1090
ApoI RAATTY 5 cut(s) 170, 845, 909, 957, 1396
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AsiGI ACCGGT 1 cut(s) 2323
Asp700I GAANNNNTTC 1 cut(s) 2528
Asp718I GGTACC 1 cut(s) 2374
AspS9I GGNCC 4 cut(s) 25, 80, 968, 2246
AvaII GGWCC 2 cut(s) 25, 2246
AxyI CCTNAGG 2 cut(s) 597, 1212
BalI TGGCCA 1 cut(s) 1092
BanI GGYRCC 2 cut(s) 1868, 2374
BanII GRGCYC 1 cut(s) 819
BarI GAAGNNNNNNTAC 2 cut(s) 1752, 1784
BauI CACGAG 2 cut(s) 1494, 2211
BbsI GAAGAC 2 cut(s) 2409, 2483
Bbv12I GWGCWC 1 cut(s) 819
BceAI ACGGC 2 cut(s) 622, 2031
BciT130I CCWGG 2 cut(s) 1094, 2501
BcoDI GTCTC 3 cut(s) 508, 1833, 2421
BfaI CTAG 4 cut(s) 695, 1575, 2651, 2681
BfmI CTRYAG 3 cut(s) 543, 1142, 1812
BfuAI ACCTGC 1 cut(s) 2509
BglII AGATCT 2 cut(s) 805, 2141
BlpI GCTNAGC 2 cut(s) 825, 2571
BmcAI AGTACT 1 cut(s) 1513
Bme1390I CCNGG 2 cut(s) 1094, 2501
Bme18I GGWCC 2 cut(s) 25, 2246
BmeRI GACNNNNNGTC 1 cut(s) 2668
BmgT120I GGNCC 4 cut(s) 25, 80, 968, 2246
BmiI GGNNCC 4 cut(s) 115, 1870, 2335, 2376
BmrFI CCNGG 2 cut(s) 1094, 2501
BmsI GCATC 7 cut(s) 204, 894, 1005, 1375, 1551, 1733, 2267
BpiI GAAGAC 2 cut(s) 2409, 2483
BpmI CTGGAG 4 cut(s) 344, 1213, 2034, 2610
Bpu1102I GCTNAGC 2 cut(s) 825, 2571
Bsa29I ATCGAT 1 cut(s) 877
BsaHI GRCGYC 3 cut(s) 12, 57, 2698
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 6 cut(s) 21, 198, 528, 1299, 2202, 2499
BsaWI WCCGGW 4 cut(s) 1463, 1771, 2323, 2717
BsaXI ACNNNNNCTCC 2 cut(s) 2007, 2037
Bsc4I CCNNNNNNNGG 3 cut(s) 1913, 2330, 2717
Bse118I RCCGGY 3 cut(s) 757, 2323, 2575
Bse1I ACTGG 7 cut(s) 466, 477, 1093, 1196, 1519, 1918, 2017
Bse21I CCTNAGG 2 cut(s) 597, 1212
Bse3DI GCAATG 1 cut(s) 1987
BseBI CCWGG 2 cut(s) 1094, 2501
BseCI ATCGAT 1 cut(s) 877
BseDI CCNNGG 6 cut(s) 21, 198, 528, 1299, 2202, 2499
BseGI GGATG 6 cut(s) 688, 745, 1489, 1510, 2401, 2605
BseLI CCNNNNNNNGG 3 cut(s) 1913, 2330, 2717
BseMI GCAATG 1 cut(s) 1987
BseMII CTCAG 6 cut(s) 611, 1203, 1457, 1844, 2352, 2634
BseNI ACTGG 7 cut(s) 466, 477, 1093, 1196, 1519, 1918, 2017
BseRI GAGGAG 9 cut(s) 79, 82, 106, 392, 496, 617, 809, 1229, 2375
BseX3I CGGCCG 1 cut(s) 389
BseYI CCCAGC 1 cut(s) 2042
BsgI GTGCAG 2 cut(s) 1518, 2094
Bsh1236I CGCG 3 cut(s) 121, 287, 551
Bsh1285I CGRYCG 3 cut(s) 186, 392, 855
BshFI GGCC 6 cut(s) 81, 383, 391, 595, 970, 1092
BshNI GGYRCC 2 cut(s) 1868, 2374
BshTI ACCGGT 1 cut(s) 2323
BshVI ATCGAT 1 cut(s) 877
BsiEI CGRYCG 3 cut(s) 186, 392, 855
BsiHKAI GWGCWC 1 cut(s) 819
BsiSI CCGG 8 cut(s) 111, 758, 1464, 1772, 2324, 2357, 2576, 2718
BsiWI CGTACG 1 cut(s) 288
BslFI GGGAC 3 cut(s) 164, 1905, 2482
BslI CCNNNNNNNGG 3 cut(s) 1913, 2330, 2717
BsmAI GTCTC 3 cut(s) 508, 1833, 2421
BsmBI CGTCTC 1 cut(s) 1833
BsmFI GGGAC 3 cut(s) 164, 1905, 2482
BsmI GAATGC 2 cut(s) 585, 1189
BsnI GGCC 6 cut(s) 81, 383, 391, 595, 970, 1092
Bso31I GGTCTC 1 cut(s) 508
Bsp1286I GDGCHC 1 cut(s) 819
Bsp143I GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
Bsp1720I GCTNAGC 2 cut(s) 825, 2571
Bsp68I TCGCGA 2 cut(s) 121, 551
BspANI GGCC 6 cut(s) 81, 383, 391, 595, 970, 1092
BspCNI CTCAG 6 cut(s) 610, 1204, 1456, 1843, 2351, 2633
BspDI ATCGAT 1 cut(s) 877
BspFNI CGCG 3 cut(s) 121, 287, 551
BspLI GGNNCC 4 cut(s) 115, 1870, 2335, 2376
BspMAI CTGCAG 1 cut(s) 547
BspMI ACCTGC 1 cut(s) 2509
BspPI GGATC 3 cut(s) 472, 1762, 1782
BspQI GCTCTTC 1 cut(s) 2603
BspT107I GGYRCC 2 cut(s) 1868, 2374
BspTNI GGTCTC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 1987
BsrFI RCCGGY 3 cut(s) 757, 2323, 2575
BsrI ACTGG 7 cut(s) 466, 477, 1093, 1196, 1519, 1918, 2017
BssAI RCCGGY 3 cut(s) 757, 2323, 2575
BssECI CCNNGG 6 cut(s) 21, 198, 528, 1299, 2202, 2499
BssMI GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
BssNI GRCGYC 3 cut(s) 12, 57, 2698
BssSI CACGAG 2 cut(s) 1494, 2211
BssT1I CCWWGG 4 cut(s) 198, 528, 1299, 2202
Bst2BI CACGAG 2 cut(s) 1494, 2211
Bst2UI CCWGG 2 cut(s) 1094, 2501
Bst4CI ACNGT 7 cut(s) 22, 244, 674, 681, 1284, 1949, 2064
Bst6I CTCTTC 3 cut(s) 1545, 1725, 2603
BstACI GRCGYC 3 cut(s) 12, 57, 2698
BstAPI GCANNNNNTGC 2 cut(s) 214, 1868
BstC8I GCNNGC 3 cut(s) 819, 1452, 1686
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 6 cut(s) 688, 745, 1489, 1510, 2401, 2605
BstFNI CGCG 3 cut(s) 121, 287, 551
BstKTI GATC 8 cut(s) 186, 467, 808, 881, 1030, 1757, 1777, 2144
BstMAI GTCTC 3 cut(s) 508, 1833, 2421
BstMBI GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
BstMCI CGRYCG 3 cut(s) 186, 392, 855
BstNI CCWGG 2 cut(s) 1094, 2501
BstNSI RCATGY 2 cut(s) 650, 1715
BstSCI CCNGG 2 cut(s) 1092, 2499
BstSFI CTRYAG 3 cut(s) 543, 1142, 1812
BstUI CGCG 3 cut(s) 121, 287, 551
BstV2I GAAGAC 2 cut(s) 2409, 2483
BstX2I RGATCY 3 cut(s) 805, 1754, 2141
BstXI CCANNNNNNTGG 2 cut(s) 2385, 2507
BstYI RGATCY 3 cut(s) 805, 1754, 2141
BstZI CGGCCG 1 cut(s) 389
Bsu15I ATCGAT 1 cut(s) 877
Bsu36I CCTNAGG 2 cut(s) 597, 1212
BsuRI GGCC 6 cut(s) 81, 383, 391, 595, 970, 1092
BsuTUI ATCGAT 1 cut(s) 877
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 6 cut(s) 688, 745, 1489, 1510, 2401, 2605
BtsIMutI CAGTG 5 cut(s) 484, 1935, 1954, 2060, 2154
BtuMI TCGCGA 2 cut(s) 121, 551
BveI ACCTGC 1 cut(s) 2509
Cac8I GCNNGC 3 cut(s) 819, 1452, 1686
Cfr10I RCCGGY 3 cut(s) 757, 2323, 2575
Cfr13I GGNCC 4 cut(s) 25, 80, 968, 2246
ClaI ATCGAT 1 cut(s) 877
CseI GACGC 4 cut(s) 20, 65, 293, 2706
Csp6I GTAC 4 cut(s) 289, 1512, 1612, 2375
CspAI ACCGGT 1 cut(s) 2323
CspCI CAANNNNNGTGG 2 cut(s) 2469, 2504
CviAII CATG 8 cut(s) 8, 647, 962, 1019, 1039, 1424, 1712, 1838
CviQI GTAC 4 cut(s) 289, 1512, 1612, 2375
DpnI GATC 8 cut(s) 185, 466, 807, 880, 1029, 1756, 1776, 2143
DpnII GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
DriI GACNNNNNGTC 1 cut(s) 2668
EaeI YGGCCR 2 cut(s) 389, 1090
EagI CGGCCG 1 cut(s) 389
Eam1104I CTCTTC 3 cut(s) 1545, 1725, 2603
Eam1105I GACNNNNNGTC 1 cut(s) 2668
EarI CTCTTC 3 cut(s) 1545, 1725, 2603
EciI GGCGGA 2 cut(s) 24, 389
Ecl136II GAGCTC 1 cut(s) 817
EclXI CGGCCG 1 cut(s) 389
Eco130I CCWWGG 4 cut(s) 198, 528, 1299, 2202
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 819
Eco31I GGTCTC 1 cut(s) 508
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 2 cut(s) 25, 2246
Eco52I CGGCCG 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 817
Eco57I CTGAAG 6 cut(s) 968, 1721, 1749, 1779, 2130, 2262
Eco81I CCTNAGG 2 cut(s) 597, 1212
EcoICRI GAGCTC 1 cut(s) 817
EcoO109I RGGNCCY 1 cut(s) 80
EcoRI GAATTC 2 cut(s) 170, 957
EcoRII CCWGG 2 cut(s) 1092, 2499
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 4 cut(s) 198, 528, 1299, 2202
EcoT22I ATGCAT 2 cut(s) 1020, 1368
EcoT38I GRGCYC 1 cut(s) 819
ErhI CCWWGG 4 cut(s) 198, 528, 1299, 2202
Esp3I CGTCTC 1 cut(s) 1833
FaeI CATG 8 cut(s) 11, 650, 965, 1022, 1042, 1427, 1715, 1841
FalI AAGNNNNNCTT 2 cut(s) 2235, 2267
FaqI GGGAC 3 cut(s) 164, 1905, 2482
FatI CATG 8 cut(s) 7, 646, 961, 1018, 1038, 1423, 1711, 1837
FauI CCCGC 3 cut(s) 1423, 2171, 2617
FauNDI CATATG 1 cut(s) 1260
FblI GTMKAC 1 cut(s) 458
FokI GGATG 6 cut(s) 675, 752, 1496, 1517, 2408, 2612
FriOI GRGCYC 1 cut(s) 819
FspBI CTAG 4 cut(s) 695, 1575, 2651, 2681
GsaI CCCAGC 1 cut(s) 2046
GsuI CTGGAG 4 cut(s) 344, 1213, 2034, 2610
HaeIII GGCC 6 cut(s) 81, 383, 391, 595, 970, 1092
HapII CCGG 8 cut(s) 111, 758, 1464, 1772, 2324, 2357, 2576, 2718
HgaI GACGC 4 cut(s) 20, 65, 293, 2706
Hin1I GRCGYC 3 cut(s) 12, 57, 2698
Hin1II CATG 8 cut(s) 11, 650, 965, 1022, 1042, 1427, 1715, 1841
HincII GTYRAC 2 cut(s) 677, 724
HindII GTYRAC 2 cut(s) 677, 724
HindIII AAGCTT 4 cut(s) 701, 785, 950, 2703
HpaII CCGG 8 cut(s) 111, 758, 1464, 1772, 2324, 2357, 2576, 2718
HpyAV CCTTC 7 cut(s) 361, 445, 511, 1696, 1964, 1999, 2237
HpyCH4III ACNGT 7 cut(s) 22, 244, 674, 681, 1284, 1949, 2064
HpyCH4IV ACGT 1 cut(s) 1051
HpySE526I ACGT 1 cut(s) 1051
Hsp92I GRCGYC 3 cut(s) 12, 57, 2698
Hsp92II CATG 8 cut(s) 11, 650, 965, 1022, 1042, 1427, 1715, 1841
KpnI GGTACC 1 cut(s) 2378
Kzo9I GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
LguI GCTCTTC 1 cut(s) 2603
LmnI GCTCC 5 cut(s) 119, 834, 1022, 2567, 2591
LweI GCATC 7 cut(s) 204, 894, 1005, 1375, 1551, 1733, 2267
MaeI CTAG 4 cut(s) 695, 1575, 2651, 2681
MaeII ACGT 1 cut(s) 1051
MaeIII GTNAC 6 cut(s) 16, 1159, 1567, 1767, 2008, 2282
MalI GATC 8 cut(s) 185, 466, 807, 880, 1029, 1756, 1776, 2143
MboI GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 3 cut(s) 805, 1754, 2141
MhlI GDGCHC 1 cut(s) 819
MlsI TGGCCA 1 cut(s) 1092
MluI ACGCGT 1 cut(s) 285
MluNI TGGCCA 1 cut(s) 1092
MlyI GAGTC 5 cut(s) 186, 329, 755, 779, 2560
MmeI TCCRAC 2 cut(s) 78, 2109
Mox20I TGGCCA 1 cut(s) 1092
Mph1103I ATGCAT 2 cut(s) 1020, 1368
MroXI GAANNNNTTC 1 cut(s) 2528
MscI TGGCCA 1 cut(s) 1092
MseI TTAA 8 cut(s) 303, 609, 621, 840, 1271, 1583, 2463, 2615
MslI CAYNNNNRTG 4 cut(s) 1263, 1285, 1371, 1928
Msp20I TGGCCA 1 cut(s) 1092
MspA1I CMGCKG 1 cut(s) 2624
MspI CCGG 8 cut(s) 111, 758, 1464, 1772, 2324, 2357, 2576, 2718
MspR9I CCNGG 2 cut(s) 1094, 2501
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 2 cut(s) 585, 1189
MvaI CCWGG 2 cut(s) 1094, 2501
MvnI CGCG 3 cut(s) 121, 287, 551
NdeI CATATG 1 cut(s) 1260
NdeII GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
NlaIII CATG 8 cut(s) 11, 650, 965, 1022, 1042, 1427, 1715, 1841
NlaIV GGNNCC 4 cut(s) 115, 1870, 2335, 2376
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 3 cut(s) 16, 1567, 2282
NruI TCGCGA 2 cut(s) 121, 551
NsiI ATGCAT 2 cut(s) 1020, 1368
NspI RCATGY 2 cut(s) 650, 1715
PaqCI CACCTGC 1 cut(s) 2509
PceI AGGCCT 1 cut(s) 595
PciSI GCTCTTC 1 cut(s) 2603
PcsI WCGNNNNNNNCGW 5 cut(s) 18, 230, 279, 288, 297
PctI GAATGC 2 cut(s) 585, 1189
PdmI GAANNNNTTC 1 cut(s) 2528
PfeI GAWTC 9 cut(s) 123, 203, 491, 1304, 1933, 1997, 2306, 2369, 2504
Pfl23II CGTACG 1 cut(s) 288
PflFI GACNNNGTC 1 cut(s) 49
PinAI ACCGGT 1 cut(s) 2323
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 5 cut(s) 185, 329, 754, 778, 2559
PpsI GAGTC 5 cut(s) 185, 329, 754, 778, 2559
Psp124BI GAGCTC 1 cut(s) 819
Psp6I CCWGG 2 cut(s) 1092, 2499
PspFI CCCAGC 1 cut(s) 2042
PspGI CCWGG 2 cut(s) 1092, 2499
PspLI CGTACG 1 cut(s) 288
PspN4I GGNNCC 4 cut(s) 115, 1870, 2335, 2376
PspPI GGNCC 4 cut(s) 25, 80, 968, 2246
PsrI GAACNNNNNNTAC 2 cut(s) 1615, 1647
PstI CTGCAG 1 cut(s) 547
PsuI RGATCY 3 cut(s) 805, 1754, 2141
PsyI GACNNNGTC 1 cut(s) 49
PvuI CGATCG 1 cut(s) 186
RruI TCGCGA 2 cut(s) 121, 551
RsaI GTAC 4 cut(s) 290, 1513, 1613, 2376
RsaNI GTAC 4 cut(s) 289, 1512, 1612, 2375
RseI CAYNNNNRTG 4 cut(s) 1263, 1285, 1371, 1928
SacI GAGCTC 1 cut(s) 819
SapI GCTCTTC 1 cut(s) 2603
SaqAI TTAA 8 cut(s) 303, 609, 621, 840, 1271, 1583, 2463, 2615
Sau3AI GATC 8 cut(s) 183, 464, 805, 878, 1027, 1754, 1774, 2141
Sau96I GGNCC 4 cut(s) 25, 80, 968, 2246
ScaI AGTACT 1 cut(s) 1513
SchI GAGTC 5 cut(s) 186, 329, 755, 779, 2560
ScrFI CCNGG 2 cut(s) 1094, 2501
SduI GDGCHC 1 cut(s) 819
SfaNI GCATC 7 cut(s) 204, 894, 1005, 1375, 1551, 1733, 2267
SfcI CTRYAG 3 cut(s) 543, 1142, 1812
SinI GGWCC 2 cut(s) 25, 2246
SmiMI CAYNNNNRTG 4 cut(s) 1263, 1285, 1371, 1928
SseBI AGGCCT 1 cut(s) 595
SspMI CTAG 4 cut(s) 695, 1575, 2651, 2681
SstI GAGCTC 1 cut(s) 819
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 2 cut(s) 1092, 2499
StyI CCWWGG 4 cut(s) 198, 528, 1299, 2202
TaaI ACNGT 7 cut(s) 22, 244, 674, 681, 1284, 1949, 2064
TaiI ACGT 1 cut(s) 1054
TatI WGTACW 1 cut(s) 1511
TauI GCSGC 5 cut(s) 40, 43, 391, 404, 476
TfiI GAWTC 9 cut(s) 123, 203, 491, 1304, 1933, 1997, 2306, 2369, 2504
Tru1I TTAA 8 cut(s) 303, 609, 621, 840, 1271, 1583, 2463, 2615
Tru9I TTAA 8 cut(s) 303, 609, 621, 840, 1271, 1583, 2463, 2615
TscAI CASTG 5 cut(s) 484, 1935, 1954, 2067, 2154
TseFI GTSAC 3 cut(s) 16, 1567, 2282
Tsp45I GTSAC 3 cut(s) 16, 1567, 2282
TspDTI ATGAA 6 cut(s) 563, 677, 753, 858, 950, 1521
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 5 cut(s) 484, 1935, 1954, 2067, 2154
Tth111I GACNNNGTC 1 cut(s) 49
VpaK11BI GGWCC 2 cut(s) 25, 2246
XapI RAATTY 5 cut(s) 170, 845, 909, 957, 1396
XceI RCATGY 2 cut(s) 650, 1715
XcmI CCANNNNNNNNNTGG 2 cut(s) 2200, 2507
XmiI GTMKAC 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 2528
XspI CTAG 4 cut(s) 695, 1575, 2651, 2681
ZrmI AGTACT 1 cut(s) 1513
Zsp2I ATGCAT 2 cut(s) 1020, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.