MD12G1102200.v1.1

Cactus-binding C-terminus of cactin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005193
Physical Location & Seq
Reverse (-)
16014048 .. 16015092
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1102200.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGGATTTTTGGCTTCCGCGGAGATTGAAAAGGTCAAGAAAAGAAGGGAAGAAAGGGCACTTGAGAAAGCACAACATGAGGAGGAAATGGCACTGCTAGCCAGAGAATGTGCTCGGGCTGAGTTCCAAGATTGGGAGAAAAAAGAAGAAGAGTTCCACTTCCATCAATGCAAGGTCAGGTCAGAGATCAGATTGCGAGAAGGGCGTATTAAGCCAATTGATGTACTGTCTAAACATCTCAGTGGCTCAGATGACTTTGAGATTGAGATAGATGAGCCATTTATGGTTTTCATGGCACCTGTTCTGTGCAATGAGTGGAGATTGAAAGAGAGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CactinC_cactus Cactin_mid

Protein Analysis

113

Amino Acids

13.38

Weight (kDa)

5.32

Isoelectric Point (pI)

65.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cactin_mid PF10312 35 - 98 9e-18 Conserved mid region of cactin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AccII CGCG 1 cut(s) 20
AciI CCGC 2 cut(s) 18, 20
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 2 cut(s) 29, 325
Alw21I GWGCWC 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 114
AsuNHI GCTAGC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 114
BaeGI GKGCMC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 1 cut(s) 171
BfaI CTAG 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 297
BmtI GCTAGC 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 18
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 316
BseDI CCNNGG 1 cut(s) 18
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 3 cut(s) 111, 253, 261
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 61, 115
Bsp143I GATC 1 cut(s) 186
BspACI CCGC 2 cut(s) 18, 20
BspCNI CTCAG 3 cut(s) 112, 252, 260
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 297
BspOI GCTAGC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 228
Bst6I CTCTTC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 3 cut(s) 120, 239, 247
BstDSI CCRYGG 1 cut(s) 18
BstFNI CGCG 1 cut(s) 20
BstKTI GATC 1 cut(s) 189
BstMBI GATC 1 cut(s) 186
BstMWI GCNNNNNNNGC 3 cut(s) 98, 202, 211
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 18
BtsI GCAGTG 1 cut(s) 92
BtsIMutI CAGTG 2 cut(s) 92, 247
Cac8I GCNNGC 1 cut(s) 99
Cfr42I CCGCGG 1 cut(s) 21
Csp6I GTAC 1 cut(s) 224
CviAII CATG 2 cut(s) 77, 292
CviJI RGCY 6 cut(s) 14, 101, 119, 214, 246, 277
CviKI_1 RGCY 6 cut(s) 14, 101, 119, 214, 246, 277
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 3 cut(s) 120, 239, 247
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 114
FaeI CATG 2 cut(s) 80, 295
FaiI YATR 3 cut(s) 78, 284, 293
FatI CATG 2 cut(s) 76, 291
FspBI CTAG 1 cut(s) 98
Hin1II CATG 2 cut(s) 80, 295
HinfI GANTC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 184, 191, 250
Hpy188III TCNNGA 1 cut(s) 37
HpyAV CCTTC 2 cut(s) 39, 194
HpyCH4III ACNGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 171, 309
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 202, 211
HpyF3I CTNAG 3 cut(s) 120, 239, 247
Hsp92II CATG 2 cut(s) 80, 295
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 1 cut(s) 186
LpnPI CCDG 3 cut(s) 115, 163, 312
MaeI CTAG 1 cut(s) 98
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MboII GAAGA 3 cut(s) 62, 158, 161
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 61, 115
MluCI AATT 1 cut(s) 216
MnlI CCTC 2 cut(s) 73, 76
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 20
MunI CAATTG 1 cut(s) 216
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 3 cut(s) 98, 202, 211
NdeII GATC 1 cut(s) 186
NheI GCTAGC 1 cut(s) 97
NlaIII CATG 2 cut(s) 80, 295
NlaIV GGNNCC 1 cut(s) 297
PspN4I GGNNCC 1 cut(s) 297
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 240
SacII CCGCGG 1 cut(s) 21
SaqAI TTAA 1 cut(s) 210
Sau3AI GATC 1 cut(s) 186
SduI GDGCHC 2 cut(s) 61, 115
SetI ASST 4 cut(s) 36, 177, 182, 301
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 1 cut(s) 216
SsiI CCGC 2 cut(s) 18, 20
SspMI CTAG 1 cut(s) 98
TaaI ACNGT 1 cut(s) 228
TasI AATT 1 cut(s) 216
TatI WGTACW 1 cut(s) 223
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 2 cut(s) 99, 247
TspDTI ATGAA 1 cut(s) 280
TspRI CASTG 2 cut(s) 99, 247
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.