Rorug03G0313500

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005193
Physical Location & Seq
Forward (+)
33572035 .. 33572397
363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0313500.1

Sequence Viewer

Length: 363 bp
ATGATGAGTACCAAACTACCAATGTGGAATCTCCTTACTACCTTAATTTGTCAACTTGCACATCCTCTCTTAAAGGTCTCAAGCAACGGAAGCATTGTATTCATCGCCTCCATCGCTGGTATGAAAGCTCTGCCTAGACTATCTTCCTACGCAACAACAAAAAGCACTGTTATCCAATTTCGAAGAATTTGGCATGTGAATGGGCACAGGACAATATTCGAGCATATACTGGAGCACCATGGGCTCAACACCGGGGTTAAACCTGATAATGGGAATATCTCCTCCAAATCCTACAACTTTTTTGAAATTGACCTTGTGTTTGAGTATATTACCAGTATGATTGAGAGCAACAATCTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

120

Amino Acids

13.55

Weight (kDa)

9.25

Isoelectric Point (pI)

29.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 15 - 65 2.3e-08 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 1 cut(s) 305
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 82
ApoI RAATTY 1 cut(s) 186
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AsuC2I CCSGG 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 207
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 237
BccI CCATC 1 cut(s) 119
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 135
Bme1390I CCNGG 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 251
Bpu14I TTCGAA 1 cut(s) 181
BpuEI CTTGAG 1 cut(s) 64
BpuMI CCSGG 1 cut(s) 253
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 238, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 234, 333
BseDI CCNNGG 2 cut(s) 238, 252
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 1 cut(s) 269
BseNI ACTGG 2 cut(s) 234, 333
BseRI GAGGAG 1 cut(s) 271
BseSI GKGCMC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 82
Bsp119I TTCGAA 1 cut(s) 181
Bsp1286I GDGCHC 3 cut(s) 207, 237, 246
Bsp19I CCATGG 1 cut(s) 238
BspT104I TTCGAA 1 cut(s) 181
BspTNI GGTCTC 1 cut(s) 82
BsrI ACTGG 2 cut(s) 234, 333
BssECI CCNNGG 2 cut(s) 238, 252
BssT1I CCWWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 169
BstBI TTCGAA 1 cut(s) 181
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 61
BstMAI GTCTC 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 90, 113, 241
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 238
BtgZI GCGATG 2 cut(s) 88, 97
BtsCI GGATG 1 cut(s) 61
BtsIMutI CAGTG 1 cut(s) 165
Csp6I GTAC 1 cut(s) 9
CviAII CATG 3 cut(s) 194, 239, 360
CviJI RGCY 2 cut(s) 128, 244
CviKI_1 RGCY 2 cut(s) 128, 244
CviQI GTAC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 3 cut(s) 197, 242, 363
FaiI YATR 8 cut(s) 122, 195, 225, 227, 240, 327, 338, 361
FatI CATG 3 cut(s) 193, 238, 359
FokI GGATG 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 246
FspBI CTAG 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 251
HapII CCGG 1 cut(s) 252
Hin1II CATG 3 cut(s) 197, 242, 363
HincII GTYRAC 1 cut(s) 53
HindII GTYRAC 1 cut(s) 53
HinfI GANTC 1 cut(s) 28
HpaII CCGG 1 cut(s) 252
Hpy166II GTNNAC 1 cut(s) 53
Hpy8I GTNNAC 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 113, 241
Hsp92II CATG 3 cut(s) 197, 242, 363
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 6 cut(s) 102, 193, 215, 265, 276, 346
MaeI CTAG 1 cut(s) 135
MboII GAAGA 2 cut(s) 135, 195
MhlI GDGCHC 3 cut(s) 207, 237, 246
MluCI AATT 4 cut(s) 45, 176, 186, 306
MnlI CCTC 3 cut(s) 75, 118, 292
MseI TTAA 3 cut(s) 44, 71, 258
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 253
MwoI GCNNNNNNNGC 3 cut(s) 90, 113, 241
NciI CCSGG 1 cut(s) 253
NcoI CCATGG 1 cut(s) 238
NlaIII CATG 3 cut(s) 197, 242, 363
NspI RCATGY 1 cut(s) 197
NspV TTCGAA 1 cut(s) 181
PfeI GAWTC 1 cut(s) 28
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 3 cut(s) 44, 71, 258
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 3 cut(s) 207, 237, 246
SetI ASST 5 cut(s) 44, 78, 130, 265, 315
SfuI TTCGAA 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
Sse9I AATT 4 cut(s) 45, 176, 186, 306
SspI AATATT 1 cut(s) 216
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 169
TaqI TCGA 2 cut(s) 181, 219
TasI AATT 4 cut(s) 45, 176, 186, 306
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 3 cut(s) 44, 71, 258
Tru9I TTAA 3 cut(s) 44, 71, 258
TscAI CASTG 1 cut(s) 172
TspDTI ATGAA 2 cut(s) 91, 137
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 1 cut(s) 172
XapI RAATTY 1 cut(s) 186
XceI RCATGY 1 cut(s) 197
XspI CTAG 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.