RLG00000024152

Cactus-binding C-terminus of cactin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005193
Physical Location & Seq
Forward (+)
32536033 .. 32538602
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024152

Sequence Viewer

Length: 498 bp
ATGGCTTACCGCTTAGCCCGCCTGGAAGACTGGGGCGAGACTAGCAGTGGAGGAAGTAATGATGAAGGACTCGAGCTCGAGTTGATGAAACTAAAGCAAGAAAAGGATCTAACAGCTGATAGAATTGATTTCGAGAATCAAAAAGGGGTTAAGAAGAGAAGGGAGGAAAGAGCACTTGAAAAAGCACAACATGAGGAAGAAATGGCGCTGCTTGCCAGAGAACGCACTCGAGCTGAGTTCCAAGACTGGGAGAAAAAAGAAGAAGAGTTTCACTTTGATCAAAGCAAAATGAGGTCAGAGATCAGATTGCGTGAAGGGCATATTAAGCCAATTGATGTACTTTCCAAGCATCTCAATGGCTCAGCTGATTTAGATATTGAGATAAATGAGCCATACATGGTTTTCAAGGGCTTGACAGTAAAGGAAATGGAAGAGCTTCGAGATGACATCAAAATGCATCTTGGTTTGGACAGGGCTACACCTACACATATAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CactinC_cactus Cactin_mid

Protein Analysis

166

Amino Acids

19.38

Weight (kDa)

5.19

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cactin_mid PF10312 73 - 159 3.4e-27 Conserved mid region of cactin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 10, 19
AclWI GGATC 1 cut(s) 114
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 1 cut(s) 247
AgsI TTSAA 2 cut(s) 179, 406
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 5 cut(s) 76, 116, 233, 365, 436
AluI AGCT 5 cut(s) 76, 116, 233, 365, 436
Alw21I GWGCWC 2 cut(s) 78, 175
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 1 cut(s) 114
Ama87I CYCGRG 3 cut(s) 71, 77, 228
ApeKI GCWGC 1 cut(s) 208
Asp700I GAANNNNTTC 2 cut(s) 267, 435
AspLEI GCGC 1 cut(s) 208
AvaI CYCGRG 3 cut(s) 71, 77, 228
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 78, 175
BbvI GCAGC 1 cut(s) 195
BciT130I CCWGG 1 cut(s) 23
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 209
BisI GCNGC 1 cut(s) 209
BlpI GCTNAGC 2 cut(s) 13, 361
BlsI GCNGC 1 cut(s) 210
Bme1390I CCNGG 1 cut(s) 23
BmeT110I CYCGRG 3 cut(s) 71, 77, 228
BmrFI CCNGG 1 cut(s) 23
BmrI ACTGGG 2 cut(s) 40, 256
BmsI GCATC 2 cut(s) 358, 466
BmuI ACTGGG 2 cut(s) 40, 256
BpiI GAAGAC 1 cut(s) 33
Bpu1102I GCTNAGC 2 cut(s) 13, 361
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse1I ACTGG 2 cut(s) 35, 251
BseBI CCWGG 1 cut(s) 23
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMII CTCAG 2 cut(s) 225, 375
BseNI ACTGG 2 cut(s) 35, 251
BseXI GCAGC 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 78, 175
BsiHKCI CYCGRG 3 cut(s) 71, 77, 228
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 1 cut(s) 32
BsoBI CYCGRG 3 cut(s) 71, 77, 228
Bsp1286I GDGCHC 2 cut(s) 78, 175
Bsp143I GATC 3 cut(s) 106, 277, 300
Bsp1720I GCTNAGC 2 cut(s) 13, 361
BspACI CCGC 2 cut(s) 10, 19
BspCNI CTCAG 2 cut(s) 226, 374
BspPI GGATC 1 cut(s) 114
BspQI GCTCTTC 1 cut(s) 426
BsrI ACTGG 2 cut(s) 35, 251
BssMI GATC 3 cut(s) 106, 277, 300
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 3 cut(s) 149, 258, 426
BstC8I GCNNGC 2 cut(s) 19, 213
BstDEI CTNAG 3 cut(s) 13, 234, 361
BstH2I RGCGCY 1 cut(s) 209
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 3 cut(s) 109, 280, 303
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 3 cut(s) 106, 277, 300
BstMWI GCNNNNNNNGC 5 cut(s) 18, 42, 212, 316, 325
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 195
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 19, 213
CfoI GCGC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 338
CviAII CATG 2 cut(s) 191, 397
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 3 cut(s) 13, 234, 361
DpnI GATC 3 cut(s) 108, 279, 302
DpnII GATC 3 cut(s) 106, 277, 300
Eam1104I CTCTTC 3 cut(s) 149, 258, 426
EarI CTCTTC 3 cut(s) 149, 258, 426
Ecl136II GAGCTC 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 76
Eco88I CYCGRG 3 cut(s) 71, 77, 228
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 78
FaeI CATG 2 cut(s) 194, 400
FaiI YATR 6 cut(s) 192, 321, 394, 398, 489, 491
FatI CATG 2 cut(s) 190, 396
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 1 cut(s) 209
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 209
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 207
GluI GCNGC 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 209
HhaI GCGC 1 cut(s) 208
Hin1II CATG 2 cut(s) 194, 400
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HinfI GANTC 2 cut(s) 69, 136
Hpy188I TCNGA 2 cut(s) 298, 305
Hpy188III TCNNGA 2 cut(s) 133, 440
HpyAV CCTTC 3 cut(s) 59, 153, 308
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4V TGCA 1 cut(s) 457
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 42, 212, 316, 325
HpyF3I CTNAG 3 cut(s) 13, 234, 361
Hsp92II CATG 2 cut(s) 194, 400
HspAI GCGC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 106, 277, 300
LguI GCTCTTC 1 cut(s) 426
LpnPI CCDG 6 cut(s) 8, 16, 35, 229, 232, 457
Lsp1109I GCAGC 1 cut(s) 195
LweI GCATC 2 cut(s) 358, 466
MaeI CTAG 1 cut(s) 42
MalI GATC 3 cut(s) 108, 279, 302
MboI GATC 3 cut(s) 106, 277, 300
MboII GAAGA 6 cut(s) 38, 166, 209, 272, 275, 443
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 2 cut(s) 78, 175
MluCI AATT 2 cut(s) 123, 330
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 4 cut(s) 44, 157, 187, 285
Mph1103I ATGCAT 1 cut(s) 459
MroXI GAANNNNTTC 2 cut(s) 267, 435
MseI TTAA 2 cut(s) 150, 324
MslI CAYNNNNRTG 2 cut(s) 354, 452
MspA1I CMGCKG 2 cut(s) 116, 365
MspR9I CCNGG 1 cut(s) 23
MunI CAATTG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 23
MwoI GCNNNNNNNGC 5 cut(s) 18, 42, 212, 316, 325
NdeII GATC 3 cut(s) 106, 277, 300
NlaIII CATG 2 cut(s) 194, 400
NsiI ATGCAT 1 cut(s) 459
PaeR7I CTCGAG 3 cut(s) 71, 77, 228
PciSI GCTCTTC 1 cut(s) 426
PdmI GAANNNNTTC 2 cut(s) 267, 435
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 1 cut(s) 210
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspXI VCTCGAGB 3 cut(s) 71, 77, 228
PsuI RGATCY 1 cut(s) 106
PvuII CAGCTG 2 cut(s) 116, 365
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
RseI CAYNNNNRTG 2 cut(s) 354, 452
SacI GAGCTC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 426
SaqAI TTAA 2 cut(s) 150, 324
SatI GCNGC 1 cut(s) 209
Sau3AI GATC 3 cut(s) 106, 277, 300
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 2 cut(s) 78, 175
SetI ASST 7 cut(s) 78, 118, 235, 296, 367, 438, 484
SfaNI GCATC 2 cut(s) 358, 466
Sfr274I CTCGAG 3 cut(s) 71, 77, 228
SlaI CTCGAG 3 cut(s) 71, 77, 228
SmiMI CAYNNNNRTG 2 cut(s) 354, 452
SmlI CTYRAG 3 cut(s) 71, 77, 228
SmoI CTYRAG 3 cut(s) 71, 77, 228
Sse9I AATT 2 cut(s) 123, 330
SsiI CCGC 2 cut(s) 10, 19
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 418
TaqI TCGA 5 cut(s) 72, 78, 132, 229, 439
TasI AATT 2 cut(s) 123, 330
TatI WGTACW 1 cut(s) 337
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 2 cut(s) 150, 324
Tru9I TTAA 2 cut(s) 150, 324
TscAI CASTG 1 cut(s) 52
TseI GCWGC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 78, 101
TspRI CASTG 1 cut(s) 52
XhoI CTCGAG 3 cut(s) 71, 77, 228
XmnI GAANNNNTTC 2 cut(s) 267, 435
XspI CTAG 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.