Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001861

Physical Location & Seq

Genomic Coordinates Reverse (-)

2676110 .. 2680313

Gene Structure 4204 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1039300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1683 bp

ATGGCCGCCGGCAAGGAGTTCATGGTTTACAACTCGATGACCCGCCAGAAGGAGACGTTCAGGCCGATAGAACCCGGCAAGGTCAGCATGTACGTCTGTGGAATTACCGCCTATGATCTCAGCCATCTCGGCCACGCTCGCGCCGCCGTCAATTTCGATGTCCTCTTCAGATACTTAAAGCATTTGGGTTACGAGGTTACGTATGTCCGGAATTTCACTGATGTTGATGACAAGATAATAATGCGAGCAAATAAGAATGGTGAAGACCCATTTAGTTTAAGTAGTCGTTTTTGCAAAGAATATCTCAATGACATGGGCGATCTCCAGTGCCTTCTTCCCACCCATCAGCCATATGTTTCTGAGCACATAGAACATATCAAGGATTTGATAACACAGATTATTAACAAAGAATATGCATATCTGGTTGGCGGGGATGTTTTCTTTGCTGTTGATAAATTTCCAAATTATGGCCAGTTATCTGGACAAAAATTGGAACACAATCGGGCTGGTGAGCGAGTTGCTGTTGATTCAAGGAAGCGTAATCCTGCAGACTTTGCATTGTGGAAGTCTGCAAAGCCTGGTGAGCCAAGTTGGGAAAGCCCTTGGGGACCTGGTAGACCTGGATGGCACATTGAATGCAGTGCAATGAGTGCACATTATCTGACATTCAAGTTTGATATTCATGGCGGCGGCATTGACTTGATTTTTCCACATCATGAGAATGAGGTTGCCCAGAGTTGTGCTGCATGCCAAGAGAGCAGTGTGAGTTACTGGATGCATAATGGACATGTTACCAATAACAACGAGAAAATGTCAAAATCCCTCAACAACTTTTTCACTATTAGGGAGATTACTGAAAAGTACCATCCACTGGCTTTGAGACACTTCTTGATAAGCGCTCACTATAGGTCTCCCCTCAACTACACAATCTCCCAGCTGGAAAGTTCATCAGATGCTATTTATTACGTATATCAGACTTTGCAAGATTGTGAGGATGCTTTAGCTCCACTTCTAGAAGAGGGTCTGAAGGAAGGTGCAGATCAAAATGGCAAAATAGTTAAAATTACTCTGACTGCCCAAGAATGCATCAGTAAGCTAAAGAATGAGTTTGAGTCCAAAATGTGCGATGACTTAAACACTGCCCAGATATTGACTGGTGCTTTCCAAGATGCGTTGAAGTTTATAAACAGTTCTTTGAATGGACTCAAGAAGAAGCAGCAAAAGCAAAAACAGTTATCAATGGTCCAGTCACTAATCGATGTAAAGAAAGCAGTAACTGATGATGTTCTGGACGTTTTGGGTTTAAAGTCGTCCCATACATACTCGGAGGTTTTGCAGCAATTGAAAGCGAAAGCATTGGAGAGAGCAGGGATGGTGGAAAACGATGTACTGAAGAAGATTGAAGAGAGAAAACGAGCAAGGGAAAACAAAGACTTTGCAATGAGTGATCAGATCAGAGCTGATTTGACTAGAAAAGGCATTGCACTCATGGACGTGGGCAAGGAGACAATCTGGAGACCTTGTGTACGAGAGGAGCCCATCAAGGAAGAGGAAAAACAGCTGCCGACTGAAGAGGAACAAAAGGTATTGCCGGTTGAACAAAAGGAAGAGCAAAAGCAGCTGTCAAGTGAAGAAGAACACAACGCATTACCCGTCGAAAAGGAACTGGCTGTGTCGACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019538 GO:0019752 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DALR_2 tRNA-synt_1e

Protein Analysis

561

Amino Acids

63.8

Weight (kDa)

5.93

Isoelectric Point (pI)

52.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1e	PF01406	20 - 316	3.6e-127	tRNA synthetases class I (C) catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001861)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56300 AT3G56300 AT3G56300
fragaria_vesca	FvH4_2g17241 FvH4_3g19570 FvH4_3g19580 FvH4_3g19590 FvH4_4g27030 FvH4_4g27030 FvH4_4g27030
malus_domestica	MD04G1098100.v1.1 MD13G1039300.v1.1 MD16G1040200.v1.1 MD16G1076600.v1.1 MD17G1183300.v1.1
prunus_persica	Prupe.1G313900_v2.0.a1
pyrus_communis	pycom04g11630 pycom13g03380 pycom16g03490
rosa_chinensis	RchiOBHm_Chr1g0323701 RchiOBHm_Chr1g0362331 RchiOBHm_Chr2g0088831 RchiOBHm_Chr4g0434831 RchiOBHm_Chr5g0002801 RchiOBHm_Chr5g0002811
rosa_laevigata	RLG00000006644 RLG00000031061
rosa_multiflora	Rmu_sc0000633.1_g000010 Rmu_sc0013603.1_g000016
rosa_roxburghii	Rroxscaffold_1G00073040 Rroxscaffold_5G00376040
rosa_rugosa	Rorug04G0279300 Rorug04G0279300 Rorug04G0399800
rosa_samantha	Rh1DG061300 Rh3CG112600 Rh4AG335300 Rh4BG343800 Rh4CG358200 Rh4DG337700 Rh5AG022600 Rh5BG025300 Rh5CG025100 Rh5DG023700
rosa_wichuraiana	Rw4G029240 Rw5G002100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1184
AccB7I	CCANNNNNTGG	2 cut(s)	467, 871
AccI	GTMKAC	2 cut(s)	616, 1676
AccII	CGCG	1 cut(s)	141
AccIII	TCCGGA	1 cut(s)	207
AciI	CCGC	7 cut(s)	6, 43, 108, 144, 429, 687, 690
AcoI	YGGCCR	3 cut(s)	3, 130, 469
AcsI	RAATTY	2 cut(s)	211, 455
AcuI	CTGAAG	4 cut(s)	151, 1046, 1412, 1590
AfaI	GTAC	4 cut(s)	92, 863, 1389, 1527
AfeI	AGCGCT	1 cut(s)	898
AfiI	CCNNNNNNNGG	3 cut(s)	49, 467, 871
AflIII	ACRYGT	1 cut(s)	787
AgsI	TTSAA	8 cut(s)	531, 635, 670, 1177, 1198, 1345, 1403, 1598
AjiI	CACGTC	2 cut(s)	1495, 1680
AjnI	CCWGG	3 cut(s)	577, 610, 619
AluBI	AGCT	6 cut(s)	937, 1004, 1096, 1460, 1561, 1621
AluI	AGCT	6 cut(s)	937, 1004, 1096, 1460, 1561, 1621
Alw21I	GWGCWC	2 cut(s)	366, 655
Alw26I	GTCTC	5 cut(s)	47, 874, 915, 1499, 1510
Alw44I	GTGCAC	1 cut(s)	651
AlwNI	CAGNNNCTG	1 cut(s)	1277
Aor13HI	TCCGGA	1 cut(s)	207
Aor51HI	AGCGCT	1 cut(s)	898
AoxI	GGCC	4 cut(s)	3, 62, 130, 469
ApaLI	GTGCAC	1 cut(s)	651
ApeKI	GCWGC	5 cut(s)	743, 1216, 1336, 1561, 1618
ApoI	RAATTY	2 cut(s)	211, 455
AspLEI	GCGC	2 cut(s)	143, 899
AspS9I	GGNCC	2 cut(s)	608, 1243
AsuC2I	CCSGG	1 cut(s)	75
AsuHPI	GGTGA	3 cut(s)	272, 521, 593
AvaII	GGWCC	2 cut(s)	608, 1243
BaeGI	GKGCMC	1 cut(s)	655
BalI	TGGCCA	1 cut(s)	471
BanII	GRGCYC	1 cut(s)	1539
BbsI	GAAGAC	1 cut(s)	270
Bbv12I	GWGCWC	2 cut(s)	366, 655
BbvI	GCAGC	5 cut(s)	730, 1228, 1348, 1548, 1630
BccI	CCATC	6 cut(s)	132, 351, 618, 873, 1366, 1547
BceAI	ACGGC	1 cut(s)	131
BciT130I	CCWGG	3 cut(s)	579, 612, 621
BclI	TGATCA	1 cut(s)	1447
BcnI	CCSGG	1 cut(s)	75
BcoDI	GTCTC	5 cut(s)	47, 874, 915, 1499, 1510
BfaI	CTAG	2 cut(s)	1013, 1470
BfmI	CTRYAG	2 cut(s)	546, 904
BfoI	RGCGCY	1 cut(s)	900
BglI	GCCNNNNNGGC	1 cut(s)	129
BisI	GCNGC	9 cut(s)	6, 144, 688, 691, 744, 1217, 1337, 1562, 1619
BlsI	GCNGC	9 cut(s)	7, 145, 689, 692, 745, 1218, 1338, 1563, 1620
Bme1390I	CCNGG	4 cut(s)	75, 579, 612, 621
Bme18I	GGWCC	2 cut(s)	608, 1243
BmgBI	CACGTC	2 cut(s)	1495, 1680
BmgT120I	GGNCC	2 cut(s)	608, 1243
BmiI	GGNNCC	2 cut(s)	609, 1536
BmrFI	CCNGG	4 cut(s)	75, 579, 612, 621
BmsI	GCATC	5 cut(s)	765, 943, 985, 1095, 1159
BpiI	GAAGAC	1 cut(s)	270
BpmI	CTGGAG	2 cut(s)	308, 1534
BpuEI	CTTGAG	1 cut(s)	1190
BpuMI	CCSGG	1 cut(s)	75
Bsa29I	ATCGAT	1 cut(s)	1257
BsaAI	YACGTR	2 cut(s)	201, 967
BsaI	GGTCTC	2 cut(s)	915, 1510
BsaJI	CCNNGG	1 cut(s)	602
BsaWI	WCCGGW	1 cut(s)	207
BsaXI	ACNNNNNCTCC	4 cut(s)	914, 944, 1507, 1537
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 467, 871
Bse118I	RCCGGY	2 cut(s)	8, 1591
Bse1I	ACTGG	7 cut(s)	325, 472, 776, 876, 1159, 1246, 1671
Bse3DI	GCAATG	3 cut(s)	651, 1446, 1479
BseAI	TCCGGA	1 cut(s)	207
BseBI	CCWGG	3 cut(s)	579, 612, 621
BseCI	ATCGAT	1 cut(s)	1257
BseDI	CCNNGG	1 cut(s)	602
BseGI	GGATG	6 cut(s)	439, 629, 780, 865, 1000, 1377
BseLI	CCNNNNNNNGG	3 cut(s)	49, 467, 871
BseMI	GCAATG	3 cut(s)	651, 1446, 1479
BseMII	CTCAG	2 cut(s)	133, 351
BseNI	ACTGG	7 cut(s)	325, 472, 776, 876, 1159, 1246, 1671
BseRI	GAGGAG	1 cut(s)	1547
BseSI	GKGCMC	1 cut(s)	655
BseXI	GCAGC	5 cut(s)	730, 1228, 1348, 1548, 1630
BseYI	CCCAGC	1 cut(s)	933
BsgI	GTGCAG	1 cut(s)	1056
Bsh1236I	CGCG	1 cut(s)	141
BshFI	GGCC	4 cut(s)	5, 64, 132, 471
BshVI	ATCGAT	1 cut(s)	1257
BsiHKAI	GWGCWC	2 cut(s)	366, 655
BsiSI	CCGG	4 cut(s)	9, 75, 208, 1592
BslFI	GGGAC	2 cut(s)	621, 1297
BslI	CCNNNNNNNGG	3 cut(s)	49, 467, 871
BsmAI	GTCTC	5 cut(s)	47, 874, 915, 1499, 1510
BsmBI	CGTCTC	1 cut(s)	47
BsmFI	GGGAC	2 cut(s)	621, 1297
BsmI	GAATGC	2 cut(s)	641, 1088
BsnI	GGCC	4 cut(s)	5, 64, 132, 471
Bso31I	GGTCTC	2 cut(s)	915, 1510
Bsp1286I	GDGCHC	3 cut(s)	366, 655, 1539
Bsp13I	TCCGGA	1 cut(s)	207
Bsp143I	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
BspACI	CCGC	7 cut(s)	6, 43, 108, 144, 429, 687, 690
BspANI	GGCC	4 cut(s)	5, 64, 132, 471
BspCNI	CTCAG	2 cut(s)	132, 352
BspDI	ATCGAT	1 cut(s)	1257
BspEI	TCCGGA	1 cut(s)	207
BspFNI	CGCG	1 cut(s)	141
BspHI	TCATGA	1 cut(s)	715
BspLI	GGNNCC	2 cut(s)	609, 1536
BspMAI	CTGCAG	1 cut(s)	550
BspQI	GCTCTTC	1 cut(s)	1602
BspTNI	GGTCTC	2 cut(s)	915, 1510
BsrDI	GCAATG	3 cut(s)	651, 1446, 1479
BsrFI	RCCGGY	2 cut(s)	8, 1591
BsrI	ACTGG	7 cut(s)	325, 472, 776, 876, 1159, 1246, 1671
BssAI	RCCGGY	2 cut(s)	8, 1591
BssECI	CCNNGG	1 cut(s)	602
BssMI	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
BssT1I	CCWWGG	1 cut(s)	602
Bst2UI	CCWGG	3 cut(s)	579, 612, 621
Bst4CI	ACNGT	2 cut(s)	1190, 1233
Bst6I	CTCTTC	6 cut(s)	170, 1011, 1398, 1542, 1566, 1602
BstAPI	GCANNNNNTGC	2 cut(s)	554, 650
BstBAI	YACGTR	2 cut(s)	201, 967
BstC8I	GCNNGC	4 cut(s)	10, 139, 246, 748
BstDEI	CTNAG	2 cut(s)	119, 360
BstF5I	GGATG	6 cut(s)	439, 629, 780, 865, 1000, 1377
BstFNI	CGCG	1 cut(s)	141
BstH2I	RGCGCY	1 cut(s)	900
BstHHI	GCGC	2 cut(s)	143, 899
BstKTI	GATC	5 cut(s)	118, 322, 1042, 1450, 1455
BstMAI	GTCTC	5 cut(s)	47, 874, 915, 1499, 1510
BstMBI	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
BstNI	CCWGG	3 cut(s)	579, 612, 621
BstNSI	RCATGY	3 cut(s)	91, 750, 791
BstSCI	CCNGG	4 cut(s)	73, 577, 610, 619
BstSFI	CTRYAG	2 cut(s)	546, 904
BstSLI	GKGCMC	1 cut(s)	655
BstSNI	TACGTA	2 cut(s)	201, 967
BstUI	CGCG	1 cut(s)	141
BstV1I	GCAGC	5 cut(s)	730, 1228, 1348, 1548, 1630
BstV2I	GAAGAC	1 cut(s)	270
BstXI	CCANNNNNNTGG	1 cut(s)	479
Bsu15I	ATCGAT	1 cut(s)	1257
BsuRI	GGCC	4 cut(s)	5, 64, 132, 471
BsuTUI	ATCGAT	1 cut(s)	1257
BtgZI	GCGATG	1 cut(s)	1140
BtrI	CACGTC	2 cut(s)	1495, 1680
BtsCI	GGATG	6 cut(s)	439, 629, 780, 865, 1000, 1377
BtsI	GCAGTG	3 cut(s)	646, 766, 1137
BtsIMutI	CAGTG	6 cut(s)	216, 332, 646, 766, 869, 1137
Cac8I	GCNNGC	4 cut(s)	10, 139, 246, 748
CaiI	CAGNNNCTG	1 cut(s)	1277
CciI	TCATGA	1 cut(s)	715
CfoI	GCGC	2 cut(s)	143, 899
Cfr10I	RCCGGY	2 cut(s)	8, 1591
Cfr13I	GGNCC	2 cut(s)	608, 1243
ClaI	ATCGAT	1 cut(s)	1257
CsiI	ACCWGGT	1 cut(s)	610
Csp6I	GTAC	4 cut(s)	91, 862, 1388, 1526
CspCI	CAANNNNNGTGG	2 cut(s)	858, 893
CviAII	CATG	8 cut(s)	22, 88, 313, 683, 716, 747, 788, 1489
CviQI	GTAC	4 cut(s)	91, 862, 1388, 1526
DdeI	CTNAG	2 cut(s)	119, 360
DpnI	GATC	5 cut(s)	117, 321, 1041, 1449, 1454
DpnII	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
DraI	TTTAAA	1 cut(s)	1305
EaeI	YGGCCR	3 cut(s)	3, 130, 469
Eam1104I	CTCTTC	6 cut(s)	170, 1011, 1398, 1542, 1566, 1602
EarI	CTCTTC	6 cut(s)	170, 1011, 1398, 1542, 1566, 1602
Eco105I	TACGTA	2 cut(s)	201, 967
Eco130I	CCWWGG	1 cut(s)	602
Eco24I	GRGCYC	1 cut(s)	1539
Eco31I	GGTCTC	2 cut(s)	915, 1510
Eco47I	GGWCC	2 cut(s)	608, 1243
Eco47III	AGCGCT	1 cut(s)	898
Eco57I	CTGAAG	4 cut(s)	151, 1046, 1412, 1590
EcoO109I	RGGNCCY	1 cut(s)	608
EcoRII	CCWGG	3 cut(s)	577, 610, 619
EcoT14I	CCWWGG	1 cut(s)	602
EcoT22I	ATGCAT	3 cut(s)	418, 780, 1088
EcoT38I	GRGCYC	1 cut(s)	1539
ErhI	CCWWGG	1 cut(s)	602
Esp3I	CGTCTC	1 cut(s)	47
FaeI	CATG	8 cut(s)	25, 91, 316, 686, 719, 750, 791, 1492
FaqI	GGGAC	2 cut(s)	621, 1297
FatI	CATG	8 cut(s)	21, 87, 312, 682, 715, 746, 787, 1488
FauI	CCCGC	2 cut(s)	50, 422
FauNDI	CATATG	1 cut(s)	352
FbaI	TGATCA	1 cut(s)	1447
FblI	GTMKAC	2 cut(s)	616, 1676
Fnu4HI	GCNGC	9 cut(s)	6, 144, 688, 691, 744, 1217, 1337, 1562, 1619
FokI	GGATG	6 cut(s)	446, 636, 787, 852, 1007, 1384
FriOI	GRGCYC	1 cut(s)	1539
Fsp4HI	GCNGC	9 cut(s)	6, 144, 688, 691, 744, 1217, 1337, 1562, 1619
FspBI	CTAG	2 cut(s)	1013, 1470
GlaI	GCGC	2 cut(s)	142, 898
GluI	GCNGC	9 cut(s)	6, 144, 688, 691, 744, 1217, 1337, 1562, 1619
GsaI	CCCAGC	1 cut(s)	937
GsuI	CTGGAG	2 cut(s)	308, 1534
HaeII	RGCGCY	1 cut(s)	900
HaeIII	GGCC	4 cut(s)	5, 64, 132, 471
HapII	CCGG	4 cut(s)	9, 75, 208, 1592
HhaI	GCGC	2 cut(s)	143, 899
Hin1II	CATG	8 cut(s)	25, 91, 316, 686, 719, 750, 791, 1492
Hin6I	GCGC	2 cut(s)	141, 897
HinP1I	GCGC	2 cut(s)	141, 897
HincII	GTYRAC	1 cut(s)	1677
HindII	GTYRAC	1 cut(s)	1677
HinfI	GANTC	3 cut(s)	527, 1112, 1203
HpaII	CCGG	4 cut(s)	9, 75, 208, 1592
HphI	GGTGA	3 cut(s)	272, 521, 593
Hpy166II	GTNNAC	5 cut(s)	28, 617, 653, 1526, 1677
Hpy188III	TCNNGA	8 cut(s)	208, 480, 716, 889, 1013, 1207, 1289, 1513
Hpy8I	GTNNAC	5 cut(s)	28, 617, 653, 1526, 1677
Hpy99I	CGWCG	2 cut(s)	1658, 1681
HpyAV	CCTTC	4 cut(s)	43, 341, 1021, 1025
HpyCH4III	ACNGT	2 cut(s)	1190, 1233
HpyCH4IV	ACGT	7 cut(s)	56, 93, 200, 966, 1293, 1494, 1679
HpyF3I	CTNAG	2 cut(s)	119, 360
HpySE526I	ACGT	7 cut(s)	56, 93, 200, 966, 1293, 1494, 1679
Hsp92II	CATG	8 cut(s)	25, 91, 316, 686, 719, 750, 791, 1492
HspAI	GCGC	2 cut(s)	141, 897
Kpn2I	TCCGGA	1 cut(s)	207
KroI	GCCGGC	1 cut(s)	8
KroNI	GCCGGC	1 cut(s)	10
Ksp22I	TGATCA	1 cut(s)	1447
Kzo9I	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
LguI	GCTCTTC	1 cut(s)	1602
LmnI	GCTCC	2 cut(s)	1009, 1534
Lsp1109I	GCAGC	5 cut(s)	730, 1228, 1348, 1548, 1630
LweI	GCATC	5 cut(s)	765, 943, 985, 1095, 1159
MabI	ACCWGGT	1 cut(s)	610
MaeI	CTAG	2 cut(s)	1013, 1470
MaeII	ACGT	7 cut(s)	56, 93, 200, 966, 1293, 1494, 1679
MaeIII	GTNAC	6 cut(s)	188, 196, 767, 790, 1248, 1273
MalI	GATC	5 cut(s)	117, 321, 1041, 1449, 1454
MboI	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
MfeI	CAATTG	1 cut(s)	1340
MhlI	GDGCHC	3 cut(s)	366, 655, 1539
MlsI	TGGCCA	1 cut(s)	471
MluCI	AATT	8 cut(s)	102, 151, 211, 455, 463, 488, 1062, 1340
MluNI	TGGCCA	1 cut(s)	471
MlyI	GAGTC	2 cut(s)	1121, 1197
Mox20I	TGGCCA	1 cut(s)	471
Mph1103I	ATGCAT	3 cut(s)	418, 780, 1088
MroI	TCCGGA	1 cut(s)	207
MroNI	GCCGGC	1 cut(s)	8
MscI	TGGCCA	1 cut(s)	471
MseI	TTAA	6 cut(s)	176, 278, 402, 1059, 1133, 1304
MslI	CAYNNNNRTG	2 cut(s)	720, 1493
Msp20I	TGGCCA	1 cut(s)	471
MspA1I	CMGCKG	3 cut(s)	937, 1561, 1621
MspI	CCGG	4 cut(s)	9, 75, 208, 1592
MspR9I	CCNGG	4 cut(s)	75, 579, 612, 621
MunI	CAATTG	1 cut(s)	1340
Mva1269I	GAATGC	2 cut(s)	641, 1088
MvaI	CCWGG	3 cut(s)	579, 612, 621
MvnI	CGCG	1 cut(s)	141
NaeI	GCCGGC	1 cut(s)	10
NciI	CCSGG	1 cut(s)	75
NdeI	CATATG	1 cut(s)	352
NdeII	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
NgoMIV	GCCGGC	1 cut(s)	8
NlaIII	CATG	8 cut(s)	25, 91, 316, 686, 719, 750, 791, 1492
NlaIV	GGNNCC	2 cut(s)	609, 1536
NmeAIII	GCCGAG	1 cut(s)	108
NmuCI	GTSAC	1 cut(s)	1248
NsiI	ATGCAT	3 cut(s)	418, 780, 1088
NspI	RCATGY	3 cut(s)	91, 750, 791
PaeI	GCATGC	1 cut(s)	750
PagI	TCATGA	1 cut(s)	715
PciI	ACATGT	1 cut(s)	787
PciSI	GCTCTTC	1 cut(s)	1602
PcsI	WCGNNNNNNNCGW	2 cut(s)	62, 1650
PctI	GAATGC	2 cut(s)	641, 1088
PdiI	GCCGGC	1 cut(s)	10
PfeI	GAWTC	1 cut(s)	527
PflMI	CCANNNNNTGG	2 cut(s)	467, 871
PkrI	GCNGC	9 cut(s)	7, 145, 689, 692, 745, 1218, 1338, 1563, 1620
PleI	GAGTC	2 cut(s)	1120, 1197
PpsI	GAGTC	2 cut(s)	1120, 1197
Ppu21I	YACGTR	2 cut(s)	201, 967
PpuMI	RGGWCCY	1 cut(s)	608
PscI	ACATGT	1 cut(s)	787
PsiI	TTATAA	1 cut(s)	1184
Psp5II	RGGWCCY	1 cut(s)	608
Psp6I	CCWGG	3 cut(s)	577, 610, 619
PspFI	CCCAGC	1 cut(s)	933
PspGI	CCWGG	3 cut(s)	577, 610, 619
PspN4I	GGNNCC	2 cut(s)	609, 1536
PspPI	GGNCC	2 cut(s)	608, 1243
PspPPI	RGGWCCY	1 cut(s)	608
PstI	CTGCAG	1 cut(s)	550
PstNI	CAGNNNCTG	1 cut(s)	1277
PvuII	CAGCTG	3 cut(s)	937, 1561, 1621
RsaI	GTAC	4 cut(s)	92, 863, 1389, 1527
RsaNI	GTAC	4 cut(s)	91, 862, 1388, 1526
RseI	CAYNNNNRTG	2 cut(s)	720, 1493
SalI	GTCGAC	1 cut(s)	1675
SapI	GCTCTTC	1 cut(s)	1602
SaqAI	TTAA	6 cut(s)	176, 278, 402, 1059, 1133, 1304
SatI	GCNGC	9 cut(s)	6, 144, 688, 691, 744, 1217, 1337, 1562, 1619
Sau3AI	GATC	5 cut(s)	115, 319, 1039, 1447, 1452
Sau96I	GGNCC	2 cut(s)	608, 1243
SchI	GAGTC	2 cut(s)	1121, 1197
ScrFI	CCNGG	4 cut(s)	75, 579, 612, 621
SduI	GDGCHC	3 cut(s)	366, 655, 1539
SexAI	ACCWGGT	1 cut(s)	610
SfaNI	GCATC	5 cut(s)	765, 943, 985, 1095, 1159
SfcI	CTRYAG	2 cut(s)	546, 904
SinI	GGWCC	2 cut(s)	608, 1243
SmiMI	CAYNNNNRTG	2 cut(s)	720, 1493
SmlI	CTYRAG	1 cut(s)	1205
SmoI	CTYRAG	1 cut(s)	1205
SnaBI	TACGTA	2 cut(s)	201, 967
SphI	GCATGC	1 cut(s)	750
Sse9I	AATT	8 cut(s)	102, 151, 211, 455, 463, 488, 1062, 1340
SsiI	CCGC	7 cut(s)	6, 43, 108, 144, 429, 687, 690
SspMI	CTAG	2 cut(s)	1013, 1470
StyD4I	CCNGG	4 cut(s)	73, 577, 610, 619
StyI	CCWWGG	1 cut(s)	602
TaaI	ACNGT	2 cut(s)	1190, 1233
TaiI	ACGT	7 cut(s)	59, 96, 203, 969, 1296, 1497, 1682
TaqI	TCGA	5 cut(s)	35, 156, 1257, 1656, 1676
TasI	AATT	8 cut(s)	102, 151, 211, 455, 463, 488, 1062, 1340
TatI	WGTACW	1 cut(s)	1387
TauI	GCSGC	4 cut(s)	8, 146, 690, 693
TfiI	GAWTC	1 cut(s)	527
Tru1I	TTAA	6 cut(s)	176, 278, 402, 1059, 1133, 1304
Tru9I	TTAA	6 cut(s)	176, 278, 402, 1059, 1133, 1304
TscAI	CASTG	6 cut(s)	223, 332, 646, 766, 876, 1144
TseFI	GTSAC	1 cut(s)	1248
TseI	GCWGC	5 cut(s)	743, 1216, 1336, 1561, 1618
Tsp45I	GTSAC	1 cut(s)	1248
TspDTI	ATGAA	3 cut(s)	10, 671, 936
TspRI	CASTG	6 cut(s)	223, 332, 646, 766, 876, 1144
Van91I	CCANNNNNTGG	2 cut(s)	467, 871
VneI	GTGCAC	1 cut(s)	651
VpaK11BI	GGWCC	2 cut(s)	608, 1243
XapI	RAATTY	2 cut(s)	211, 455
XbaI	TCTAGA	1 cut(s)	1012
XceI	RCATGY	3 cut(s)	91, 750, 791
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1151
XmiI	GTMKAC	2 cut(s)	616, 1676
XspI	CTAG	2 cut(s)	1013, 1470
Zsp2I	ATGCAT	3 cut(s)	418, 780, 1088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.