Rorug04G0279300

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001861
Physical Location & Seq
Reverse (-)
45201463 .. 45216007
14545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0279300.1

Sequence Viewer

Length: 5271 bp
ATGAATTATTCAAGAGGTTTTACAGGAGCTATGGCTCTTCAAAAGTTTCCTTGTGGTGATGCACCTTCTGTTAGTATGAAACCCTTGAGCTTTCTCAGTAAGGTGCATGACAGCACTCTACATGAGGATGTAGAGACAAATCATGCCAGGGAAAAAGATGTGGATGTTGACCTCAGAGAAGTGTATTTCCTCATTATGCATTTTCTTTCAGCTGGACCATGTCATAAAACTTGTGTACAGTTCTGGAACGAACTTCTTGAACATCAACTTTTACCTAGAAGATATCATGCATGGTATTCAAGGAAAGGCATGCATAGTGGGGATGAAAATGATGAGGGGCAATCATTCCCCTTAAATTACAATATGTTGGTGGCAAGGTATCCTCACATTGAGCAGGATCACTTGGTAAAGCTTTTGAAGCAAATGATTTTGAGTACAGCTCCACCATCAAGGGGTATAAGTGGTGGAAATGCCCCTAATGCAGCTGATGTACCAACTCTCTTGGGAACGGGCTCATTTTCACTTCTGAGCTATGATAGGGATCAAGAAAACAACGAAATGAACCGGCCTGTTGCTTACATGCGTTGGCCTCACATCAAAGCTAATCAGGTGCATGGGCTAGGTTTGAGGGAAATTGGGGGAGGTTTCACTAGACATCACCGTGCACCATCTGTTCGTGCTGCATCCTATGCCATTGCGAAACCATCAACCATGTTTCAGAAGATGAAAAATACCAAGAGAATAAGGGGCCATCGAAACGCTGTCTATTGTGCAATATTTGATCGCTCAGGTAGATATGTTATCACTGGTTCAGATGATCGCCTAGTGAAGATCTGGTTAATAGAAACTGCATTTTGCTTGGCCAGCTGCCGTGGACATGAAGGTGACATCACTGATTTAGCTGTGAGCTCCAACAATGCTTTGGTGGCATCCGCTTCAAATGACTGTATCATACGAGTTTGGCGCTTGCCAGATGGGCTTCCAATTTCGGTGTTGCGGGGACACACCGGAGCTGTGACTGCAATTGCATTTAATCCCAGGCCTGGCTCTATATACCACCTTCTATCTTCCTCTGATGATGGAACTTGTAGGATATGGGATGCCAGGAATTCTCAAGTGACTCCGAGGATATACATTCCAAAACCTTCAGATACTATAACTGGGAGAAACAGTGGTCCATCCTCAAGCACTGTACAGAGCCATCAAATCTTCTGTTGTGCATTTAATGCTAATGGAACAGTCTTTGTTACCGGTAGCTCAGACACTCTTGCGAGGGTGTGGAGTGCTTCCAAGCCAGGCTCAGATGAGTCGGACCAACCAAATCATGAAATAGATGTGTTATCTGGCCATGAGAATGATGTCAATTACGTACAATTTAGTGGTTGTGCTGTAGTATCTAGATTTATGGCAGCTGACACCTCAAAGGAAGAGAATGTTCCAAAATTTAAAAATTCATGGTTCAATCATGACAATATAGTTACTTGCTCTCGCGATGGGAGTGCAATTATCTGGATTCCAAAGTCACGAAGATCACATGGAAAAGCTGGTCGTTGGACACGAGCTTATCATCTGAAAGTTCCACCTCCACCAATGCCTCCTCAACCTTCACGTGGAGGCCCCCGCCAGAGAATTCTTCCTACTCCTCGTGGTGTTAATATGATTACCTGGAGTCTTGATAATCGCTTTGTTCTTGCTGCTATCATGGATTGCAGAATATGTGTATGGAATGCATCTGATGGTAGCTTGGTCCATTCTTTAACTGGTCACAGTGATTCTACATATGTTCTGGATGTTCACCCTTTCAACCCCAGGTTGGCAATGAGTGCTGGCTATGATGGAAAAACAATTGTCTGGGATATATGGGAAGGCATGCCTATCCGGATATATCAAACTTCACAGTTCAAATTGGTTGACGGAAAGTTTTCACCGGACGGAACGTCAATTATACTATCTGATGATGTTGGCCAACTCTACATATTGGACAGTGGCCAAGGGGAGTCCCACAATGATGCAAAATATGATCAGTTCTTTCTTGGTGATTATCGCCCAATCATTCAAGATAGCTTCGGAAATGTTCTTGACCAGGAAACTCAGATTCCAGCATACCGACGAAATATGCAGGATCTACTTTGTGATTCAGGCATGATACCATATCCGGAGCCCTACCAGAGTACATACCAGAAGAGACGGCTAGGAGCTCTGGGTTCTGAGTGGTGTCCTTCCTCTTTAAAACTTGCTGTTGGGCCTGACTTCAGTCTAGATCCAGATTTCCAAATGCTACCTTTGGCAGACTTGGATATGTTATTGACCGAGCCACTACCAGAGTTTGTAGATGCCATGGATTGGGAACCAGAAAATGAAATGCGTAGTGATGATGCTGATTCAGAGTACAATATTACTGAAGATTATTCTACTGGAGGGGAGCAAGGAAGTTTAAGTTCAAACCCTTCTGTTGATCCAGACTGCAGTGCAGAAGATAGTGACGAGGATGCTCAACTGGATGGACTACGAAGATCAAGAAGGAAAAAACAAAAGGCTGATATTGAAGTCATGACGTCTTCTGGAAGGTGTGTTAAAAGGAGGAACTTGGATGAGCGTGATGATCACTCTTTCAGAAGTAATAGAACAAGGAAATCAAGGCATGGCCGAAAAGCTTCAAAAAAGAAATGCTCCGCATCAAAGTCACTGAGACCTCGAAGAGTTGCTGCACGCAATGCCCTTACACTATTTTCTAAGATAACCGGACGAGATACTGATGGAGAAGATGAAGATGGATCCGAAGGAGAAATTTCAGGTAGTGAATCCATGCTGCAAGATTCAAATATTGAGAGTGACGGATCCGATAGGGAAAATCACCAAAGCAACTATATGAAAGGAAAACAGGTTTTACTCGATGAATCTGAGGGTCTGGTCAATTCTCATGAACTTCCTGAATTTCAAAGAAACTCTGGGAACAGGAGGAGATTAGTGCTAAAACTGCCAAGACGGCCGTCAAATACAAATACGCTTGTGGATACAGATAAGTGTGGAAATCCGGCTTATTTGGGTGACCAATCTTGTAGTCATAAAGCCAGTGAAGCAAATGAGACTCATATGAGCTCTCAGGATCTGAGGAGCTCTCCTGGTCATAAAAATTGCAGCATGTTTGAAAGAGCAGTGGGAGGGCAATCAAACAAAGTTGAAAATTATTTAGACTTGACTGAGAATTACAAAGTTGGAAGTATAAGATGGGGAGGGTCCAGAGCTCGAACATTGAAGCGGCTGAGAGTGGGAGAAGCCACATCACTGGATGCACTTGCCACTACCAGTATAGCTGTTGTTGGTCACACTGAAAAAAATTACACAGAATCTGAAAAAGATTGTCAAAAAATGTCCCCTCAATTAGAAAGCCAAAGGAATGGGGATATGATGGATGGAGTGGTTATGACGAATGAGAGAACTATTGGGACTAGTACCTTTGAGGGTCTTAATGGTAAAGCCGAGGTTAATGAGCACTCAGGATTTAGTGAATGTAAGGATCATGACCAATCACCTAAATCTGTTCACATGGCTCCTTGGGGTGCAAGCACTGCCTCTTACCTCGACAAGGATGGGAGTGTTTTCTCTTCTGAACAAAACGAGAAGCTCACACCGGTCTCGACAAAATTAAGGTTGAGGAAGATTTCGAGGGGCCCTGAAAGTCCTTGCAAACAGGAAATGAGCCCTGTAGTAGAGAACTTGGAAAGCGGTAGATGTAACACATTGCCTGAAAGCCTTTCAACAATAGATCATGATTCAATTGTGCCGGAGGATAATGGCACCCATAAATTTGATTCAGATCAAAGATGCAGCGGCTCACGAGAATCAGACACTCAAATTGAATCAGTCGAGTCCCATCTAAATAGAAACAAAATGTTTAGTGCTGTTTATAGGAGGGTGAAATCACATAGGGGGCGGACTAATATAGAAGGTGAAAGTAGTGGGAAGGGAGAAGGATCTTCACAAATTTCCAACACTAGTGACCAAAATCTCATTGCTGCAGTGGACTGCCGTGACGATTCAATTGATGGAGCTCGTAGGACACGGTCCATGGGGTTGAAGGCATCTACCTGTGATGCAAGTAGTGTGGACCATGATCTCAAGTTAAGTCAAGGCCATGAGCCTAGGTATACATTCAGAAGTGCCCAGAACAACACTCTGCACAAATGTCATTTTCAGAATGAAGAACAGGGATCAAGTTCAAGGACAGCAGTGGGGTTGAGATCTACTAGAAACAGAAGGAGTTATTATGATCATGACATGCATCCAATAGATAAAAGGAAGTCGCATCAATCAACGAGGAAAGGAACATGGTTGATGTTGTCCGCACATGAGGAGAGCTCACGATATATTCCCCAGTTGGGGGATGAAGTTGTCTATTTGAGACAGGGGCATCAGGAGTATTTTGATCTTGGTAGGTTGAGAGAAGACCCACCTTGGGCATTTATAAAGCAAAGAATAAGAGCTGTGGAATTTTGTAAAGTTCAAGACCTAGAGTATTCTTTACTTCCAGGGTCTGGGGATAGCTGCTGTAAATTGACCCTTGAATTTGTAGATCCTTCATCTGATGTTTACTGCAAATCTTTCAAAATGACTCTGCCTGAAGTTACTGGTTTCCCCGATTTCATTGTTGAAAGAACTCGCTATGTTGCTGCAATTGGGAAAAAATGGTCATGTAGGGATAAGTGCAAAGTCTGGTGGAAAAATGACGGTGATGATGATGGTGGTTGGTGGGATGGCCGGGTTGTGCTTATTCAGCCCAAATCTCCCGAATTTCCAGACAGTCCATGGGAAACGTGTACGGTTCTGTACAGGACTGTTCCTAAAGAGACACAGCTACATAGTCCTTGGGAGCTTTTTGATACCAATACTCAATGGGTTCAGCCTCAGATTGATGATAAGAGCAAAAACAAGCTGCATTCTGCCTTTGCCAAACTAGAGAAATTAGCCAGCAGTCGTCAGGATTCGTTAGGGATTAATAAATTGAAGCAGCTTCAGGAGAAGCCGAAGTTCACAAATTGGTGTGCAGTTCCAATATCTCTTGACCTGATCCAGAGTAGGTTAGAAAACGACTATTATCGAAGTTTGGAAGCACTGAAGCATGACTTTAAAGTAATGTTGTTAGATGCGCAAGCCTATGTAGAAAGCCATGCGGAGTCTTCAGGCAAAACGAAACGGACAGCAGATAAAGAGTTTCTAGAGAAACTCAAATGCCTATCAGGCTGGTTCACAGAGACAATTTCGTCCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

1756

Amino Acids

197.07

Weight (kDa)

6.5

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRWD1_N PF25437 46 - 146 8.3e-35 BRWD1-like, N-terminal domain
Beta-prop_TEP1_2nd PF25047 238 - 374 2.6e-12 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 239 - 326 1e-17 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 240 - 374 5.3e-15 CDC20/Fizzy WD40 domain
WDR55 PF24796 240 - 373 2.2e-07 WDR55
Beta-prop_WDR3_1st PF25173 241 - 372 3.8e-22 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 242 - 455 1.1e-13 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 242 - 323 1.5e-10 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 243 - 323 3.5e-06 MABP1/WDR62 second WD40 domain
WD40 PF00400 244 - 279 7.3e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 246 - 322 8e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 248 - 376 1.4e-26 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 249 - 374 8.7e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 263 - 368 3e-09 SCAP Beta-propeller
WD40 PF00400 286 - 321 1.6e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 286 - 378 2.7e-08 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 291 - 375 3.8e-06 EIF3I
Beta-prop_THOC3 PF25174 293 - 376 4.4e-12 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 296 - 385 2.5e-12 WDHD1 first WD40 domain
WD40 PF00400 328 - 367 9.7e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 549 - 665 4.2e-11 WDR5 beta-propeller domain
WD40_Prp19 PF24814 550 - 662 2.6e-07 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 550 - 627 2.1e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 551 - 674 3.8e-14 THOC3 beta-propeller domain
BRWD_AD PF25313 1454 - 1629 8.2e-55 BRWD/PHIP ancillary domain-like domain
Bromodomain PF00439 1671 - 1722 1.7e-06 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4466
AasI GACNNNNNNGTC 1 cut(s) 5260
AatII GACGTC 1 cut(s) 2558
Acc16I TGCGCA 1 cut(s) 5148
AccB1I GGYRCC 1 cut(s) 3797
AccB7I CCANNNNNTGG 2 cut(s) 2343, 4535
AccI GTMKAC 1 cut(s) 4148
AccII CGCG 1 cut(s) 1491
AccIII TCCGGA 2 cut(s) 1878, 2155
AcoI YGGCCR 7 cut(s) 861, 1345, 1963, 1987, 2644, 2987, 4756
AcuI CTGAAG 7 cut(s) 1131, 2236, 2421, 4641, 4997, 5135, 5163
AcvI CACGTG 1 cut(s) 1610
AcyI GRCGYC 1 cut(s) 2555
AflIII ACRYGT 1 cut(s) 4814
AgeI ACCGGT 2 cut(s) 1250, 3631
AhdI GACNNNNNGTC 1 cut(s) 1936
AhlI ACTAGT 2 cut(s) 3449, 3995
Alw21I GWGCWC 9 cut(s) 667, 911, 2200, 3101, 3119, 3247, 3495, 4054, 4361
Alw26I GTCTC 8 cut(s) 128, 2179, 2683, 3080, 3640, 4396, 4841, 5246
Alw44I GTGCAC 1 cut(s) 663
AlwNI CAGNNNCTG 3 cut(s) 3109, 3350, 4535
Aor13HI TCCGGA 2 cut(s) 1878, 2155
ApaI GGGCCC 1 cut(s) 3674
ApaLI GTGCAC 1 cut(s) 663
AseI ATTAAT 1 cut(s) 4995
AsiGI ACCGGT 2 cut(s) 1250, 3631
Asp700I GAANNNNTTC 2 cut(s) 1921, 2653
AspA2I CCTAGG 1 cut(s) 4142
AspLEI GCGC 2 cut(s) 966, 5149
AsuC2I CCSGG 1 cut(s) 4760
AvaII GGWCC 7 cut(s) 215, 1175, 1312, 1747, 3237, 4065, 4108
AvrII CCTAGG 1 cut(s) 4142
BaeGI GKGCMC 3 cut(s) 667, 3674, 4165
BalI TGGCCA 4 cut(s) 863, 1347, 1965, 1989
BamHI GGATCC 2 cut(s) 2774, 2837
BanI GGYRCC 1 cut(s) 3797
BauI CACGAG 3 cut(s) 1557, 1644, 3837
BbrPI CACGTG 1 cut(s) 1610
BbsI GAAGAC 3 cut(s) 2550, 4452, 5169
Bbv12I GWGCWC 9 cut(s) 667, 911, 2200, 3101, 3119, 3247, 3495, 4054, 4361
BceAI ACGGC 5 cut(s) 855, 2204, 2974, 3002, 4014
BcgI CGANNNNNNTGC 2 cut(s) 1481, 1515
BciVI GTATCC 2 cut(s) 390, 3007
BclI TGATCA 3 cut(s) 2020, 2602, 4270
BcnI CCSGG 1 cut(s) 4760
BcoDI GTCTC 8 cut(s) 128, 2179, 2683, 3080, 3640, 4396, 4841, 5246
BcuI ACTAGT 2 cut(s) 3449, 3995
BfmI CTRYAG 5 cut(s) 1389, 2464, 3705, 4017, 5267
BfoI RGCGCY 1 cut(s) 967
BfuI GTATCC 2 cut(s) 390, 3007
BglI GCCNNNNNGGC 3 cut(s) 976, 2986, 5238
BglII AGATCT 2 cut(s) 831, 4241
BlnI CCTAGG 1 cut(s) 4142
Bme18I GGWCC 7 cut(s) 215, 1175, 1312, 1747, 3237, 4065, 4108
BmeRI GACNNNNNGTC 1 cut(s) 1936
BmrI ACTGGG 2 cut(s) 1170, 4369
BmuI ACTGGG 2 cut(s) 1170, 4369
BoxI GACNNNNGTC 2 cut(s) 2253, 2989
BpiI GAAGAC 3 cut(s) 2550, 4452, 5169
BplI GAGNNNNNCTC 6 cut(s) 424, 456, 3103, 3135, 4343, 4375
BpmI CTGGAG 2 cut(s) 1687, 2436
Bpu10I CCTNAGC 1 cut(s) 787
BpuEI CTTGAG 4 cut(s) 106, 1098, 1168, 4103
BpuMI CCSGG 1 cut(s) 4760
BsaAI YACGTR 2 cut(s) 1369, 1610
BsaBI GATNNNNATC 2 cut(s) 540, 3816
BsaHI GRCGYC 1 cut(s) 2555
BsaI GGTCTC 2 cut(s) 2683, 3640
BsaWI WCCGGW 7 cut(s) 1007, 1250, 1878, 1927, 2155, 2741, 3631
BsaXI ACNNNNNCTCC 2 cut(s) 1156, 1186
Bse118I RCCGGY 3 cut(s) 564, 1250, 3631
Bse3DI GCAATG 5 cut(s) 693, 1824, 2719, 3740, 4011
Bse8I GATNNNNATC 2 cut(s) 540, 3816
BseAI TCCGGA 2 cut(s) 1878, 2155
BseJI GATNNNNATC 2 cut(s) 540, 3816
BseMI GCAATG 5 cut(s) 693, 1824, 2719, 3740, 4011
BseRI GAGGAG 5 cut(s) 1587, 1632, 2974, 3127, 4367
BseSI GKGCMC 3 cut(s) 667, 3674, 4165
BseX3I CGGCCG 1 cut(s) 2987
BsgI GTGCAG 4 cut(s) 2490, 2691, 4163, 5064
Bsh1236I CGCG 1 cut(s) 1491
Bsh1285I CGRYCG 1 cut(s) 2990
BshNI GGYRCC 1 cut(s) 3797
BshTI ACCGGT 2 cut(s) 1250, 3631
BsiEI CGRYCG 1 cut(s) 2990
BsiHKAI GWGCWC 9 cut(s) 667, 911, 2200, 3101, 3119, 3247, 3495, 4054, 4361
BslFI GGGAC 6 cut(s) 1014, 1984, 3358, 3460, 3856, 5248
BsmAI GTCTC 8 cut(s) 128, 2179, 2683, 3080, 3640, 4396, 4841, 5246
BsmBI CGTCTC 1 cut(s) 2179
BsmFI GGGAC 6 cut(s) 1014, 1984, 3358, 3460, 3856, 5248
BsmI GAATGC 2 cut(s) 1732, 4936
Bso31I GGTCTC 2 cut(s) 2683, 3640
Bsp120I GGGCCC 1 cut(s) 3670
Bsp13I TCCGGA 2 cut(s) 1878, 2155
Bsp1407I TGTACA 3 cut(s) 235, 1192, 4827
Bsp19I CCATGG 3 cut(s) 2337, 4068, 4805
Bsp68I TCGCGA 1 cut(s) 1491
BspEI TCCGGA 2 cut(s) 1878, 2155
BspFNI CGCG 1 cut(s) 1491
BspHI TCATGA 7 cut(s) 1324, 1465, 2550, 2920, 3520, 3769, 4273
BspMAI CTGCAG 2 cut(s) 2468, 4021
BspQI GCTCTTC 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 3797
BspTNI GGTCTC 2 cut(s) 2683, 3640
BsrDI GCAATG 5 cut(s) 693, 1824, 2719, 3740, 4011
BsrFI RCCGGY 3 cut(s) 564, 1250, 3631
BsrGI TGTACA 3 cut(s) 235, 1192, 4827
BssAI RCCGGY 3 cut(s) 564, 1250, 3631
BssNAI GTATAC 1 cut(s) 4149
BssNI GRCGYC 1 cut(s) 2555
BssSI CACGAG 3 cut(s) 1557, 1644, 3837
BssT1I CCWWGG 8 cut(s) 1990, 2337, 3554, 4068, 4142, 4454, 4805, 4865
Bst1107I GTATAC 1 cut(s) 4149
Bst2BI CACGAG 3 cut(s) 1557, 1644, 3837
Bst6I CTCTTC 5 cut(s) 42, 1422, 2177, 2692, 3610
BstACI GRCGYC 1 cut(s) 2555
BstAPI GCANNNNNTGC 5 cut(s) 689, 1226, 1823, 2714, 3569
BstAUI TGTACA 3 cut(s) 235, 1192, 4827
BstBAI YACGTR 2 cut(s) 1369, 1610
BstC8I GCNNGC 9 cut(s) 311, 865, 968, 1828, 1871, 2710, 3565, 4969, 5151
BstDSI CCRYGG 4 cut(s) 871, 2337, 4068, 4805
BstEII GGTNACC 1 cut(s) 3047
BstENI CCTNNNNNAGG 1 cut(s) 3584
BstFNI CGCG 1 cut(s) 1491
BstH2I RGCGCY 1 cut(s) 967
BstHHI GCGC 2 cut(s) 966, 5149
BstMAI GTCTC 8 cut(s) 128, 2179, 2683, 3080, 3640, 4396, 4841, 5246
BstMCI CGRYCG 1 cut(s) 2990
BstNSI RCATGY 5 cut(s) 313, 583, 1873, 3145, 4282
BstPAI GACNNNNGTC 2 cut(s) 2253, 2989
BstPI GGTNACC 1 cut(s) 3047
BstSFI CTRYAG 5 cut(s) 1389, 2464, 3705, 4017, 5267
BstSLI GKGCMC 3 cut(s) 667, 3674, 4165
BstSNI TACGTA 1 cut(s) 1369
BstUI CGCG 1 cut(s) 1491
BstV2I GAAGAC 3 cut(s) 2550, 4452, 5169
BstX2I RGATCY 9 cut(s) 831, 2122, 2260, 2774, 2837, 3106, 3974, 4241, 4573
BstXI CCANNNNNNTGG 2 cut(s) 3286, 3398
BstYI RGATCY 9 cut(s) 831, 2122, 2260, 2774, 2837, 3106, 3974, 4241, 4573
BstZ17I GTATAC 1 cut(s) 4149
BstZI CGGCCG 1 cut(s) 2987
BsuI GTATCC 2 cut(s) 390, 3007
BtgI CCRYGG 4 cut(s) 871, 2337, 4068, 4805
BtgZI GCGATG 1 cut(s) 1506
BtsI GCAGTG 5 cut(s) 2473, 3162, 3567, 4026, 4236
BtuMI TCGCGA 1 cut(s) 1491
Cac8I GCNNGC 9 cut(s) 311, 865, 968, 1828, 1871, 2710, 3565, 4969, 5151
CaiI CAGNNNCTG 3 cut(s) 3109, 3350, 4535
CciI TCATGA 7 cut(s) 1324, 1465, 2550, 2920, 3520, 3769, 4273
CfoI GCGC 2 cut(s) 966, 5149
Cfr10I RCCGGY 3 cut(s) 564, 1250, 3631
CspAI ACCGGT 2 cut(s) 1250, 3631
CspCI CAANNNNNGTGG 2 cut(s) 4086, 4121
DraI TTTAAA 3 cut(s) 1447, 2229, 5128
DrdI GACNNNNNNGTC 1 cut(s) 5260
DriI GACNNNNNGTC 1 cut(s) 1936
DseDI GACNNNNNNGTC 1 cut(s) 5260
EaeI YGGCCR 7 cut(s) 861, 1345, 1963, 1987, 2644, 2987, 4756
EagI CGGCCG 1 cut(s) 2987
Eam1104I CTCTTC 5 cut(s) 42, 1422, 2177, 2692, 3610
Eam1105I GACNNNNNGTC 1 cut(s) 1936
EarI CTCTTC 5 cut(s) 42, 1422, 2177, 2692, 3610
EciI GGCGGA 1 cut(s) 3949
Ecl136II GAGCTC 7 cut(s) 909, 2198, 3099, 3117, 3245, 4052, 4359
EclXI CGGCCG 1 cut(s) 2987
Eco105I TACGTA 1 cut(s) 1369
Eco130I CCWWGG 8 cut(s) 1990, 2337, 3554, 4068, 4142, 4454, 4805, 4865
Eco147I AGGCCT 1 cut(s) 1042
Eco31I GGTCTC 2 cut(s) 2683, 3640
Eco32I GATATC 1 cut(s) 284
Eco47I GGWCC 7 cut(s) 215, 1175, 1312, 1747, 3237, 4065, 4108
Eco52I CGGCCG 1 cut(s) 2987
Eco53kI GAGCTC 7 cut(s) 909, 2198, 3099, 3117, 3245, 4052, 4359
Eco57I CTGAAG 7 cut(s) 1131, 2236, 2421, 4641, 4997, 5135, 5163
Eco72I CACGTG 1 cut(s) 1610
Eco91I GGTNACC 1 cut(s) 3047
EcoICRI GAGCTC 7 cut(s) 909, 2198, 3099, 3117, 3245, 4052, 4359
EcoNI CCTNNNNNAGG 1 cut(s) 3584
EcoO109I RGGNCCY 3 cut(s) 1616, 3670, 3671
EcoO65I GGTNACC 1 cut(s) 3047
EcoRI GAATTC 2 cut(s) 1108, 1629
EcoRV GATATC 1 cut(s) 284
EcoT14I CCWWGG 8 cut(s) 1990, 2337, 3554, 4068, 4142, 4454, 4805, 4865
EcoT22I ATGCAT 5 cut(s) 201, 292, 315, 1732, 4284
ErhI CCWWGG 8 cut(s) 1990, 2337, 3554, 4068, 4142, 4454, 4805, 4865
Esp3I CGTCTC 1 cut(s) 2179
FalI AAGNNNNNCTT 2 cut(s) 5108, 5140
FaqI GGGAC 6 cut(s) 1014, 1984, 3358, 3460, 3856, 5248
FauI CCCGC 2 cut(s) 990, 1628
FauNDI CATATG 2 cut(s) 1780, 3093
FbaI TGATCA 3 cut(s) 2020, 2602, 4270
FblI GTMKAC 1 cut(s) 4148
FspI TGCGCA 1 cut(s) 5148
GlaI GCGC 2 cut(s) 965, 5148
GsuI CTGGAG 2 cut(s) 1687, 2436
HaeII RGCGCY 1 cut(s) 967
HhaI GCGC 2 cut(s) 966, 5149
Hin1I GRCGYC 1 cut(s) 2555
Hin6I GCGC 2 cut(s) 964, 5147
HinP1I GCGC 2 cut(s) 964, 5147
HincII GTYRAC 2 cut(s) 169, 1912
HindII GTYRAC 2 cut(s) 169, 1912
HindIII AAGCTT 2 cut(s) 410, 2652
Hpy99I CGWCG 1 cut(s) 2112
HpyCH4IV ACGT 5 cut(s) 1368, 1609, 1937, 2555, 4814
HpySE526I ACGT 5 cut(s) 1368, 1609, 1937, 2555, 4814
Hsp92I GRCGYC 1 cut(s) 2555
HspAI GCGC 2 cut(s) 964, 5147
Kpn2I TCCGGA 2 cut(s) 1878, 2155
Ksp22I TGATCA 3 cut(s) 2020, 2602, 4270
LguI GCTCTTC 1 cut(s) 42
MaeII ACGT 5 cut(s) 1368, 1609, 1937, 2555, 4814
MfeI CAATTG 5 cut(s) 1023, 1845, 3777, 4041, 4674
MflI RGATCY 9 cut(s) 831, 2122, 2260, 2774, 2837, 3106, 3974, 4241, 4573
MlsI TGGCCA 4 cut(s) 863, 1347, 1965, 1989
MluNI TGGCCA 4 cut(s) 863, 1347, 1965, 1989
MlyI GAGTC 8 cut(s) 1114, 1316, 1678, 2006, 3082, 3878, 4606, 5183
MmeI TCCRAC 5 cut(s) 936, 1290, 1532, 3196, 4014
Mox20I TGGCCA 4 cut(s) 863, 1347, 1965, 1989
Mph1103I ATGCAT 5 cut(s) 201, 292, 315, 1732, 4284
MroI TCCGGA 2 cut(s) 1878, 2155
MroXI GAANNNNTTC 2 cut(s) 1921, 2653
MscI TGGCCA 4 cut(s) 863, 1347, 1965, 1989
MslI CAYNNNNRTG 5 cut(s) 126, 660, 882, 1353, 2007
Msp20I TGGCCA 4 cut(s) 863, 1347, 1965, 1989
MspA1I CMGCKG 5 cut(s) 212, 485, 867, 1412, 3831
MunI CAATTG 5 cut(s) 1023, 1845, 3777, 4041, 4674
Mva1269I GAATGC 2 cut(s) 1732, 4936
MvnI CGCG 1 cut(s) 1491
NciI CCSGG 1 cut(s) 4760
NcoI CCATGG 3 cut(s) 2337, 4068, 4805
NdeI CATATG 2 cut(s) 1780, 3093
NmeAIII GCCGAG 1 cut(s) 3505
NruI TCGCGA 1 cut(s) 1491
NsbI TGCGCA 1 cut(s) 5148
NsiI ATGCAT 5 cut(s) 201, 292, 315, 1732, 4284
NspI RCATGY 5 cut(s) 313, 583, 1873, 3145, 4282
PaeI GCATGC 2 cut(s) 313, 1873
PagI TCATGA 7 cut(s) 1324, 1465, 2550, 2920, 3520, 3769, 4273
PceI AGGCCT 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 1555
PctI GAATGC 2 cut(s) 1732, 4936
PdmI GAANNNNTTC 2 cut(s) 1921, 2653
PflFI GACNNNGTC 2 cut(s) 219, 4063
PflMI CCANNNNNTGG 2 cut(s) 2343, 4535
PinAI ACCGGT 2 cut(s) 1250, 3631
PleI GAGTC 8 cut(s) 1114, 1315, 1677, 2005, 3082, 3877, 4606, 5182
PmaCI CACGTG 1 cut(s) 1610
PmlI CACGTG 1 cut(s) 1610
PpsI GAGTC 8 cut(s) 1114, 1315, 1677, 2005, 3082, 3877, 4606, 5182
Ppu21I YACGTR 2 cut(s) 1369, 1610
PshAI GACNNNNGTC 2 cut(s) 2253, 2989
PshBI ATTAAT 1 cut(s) 4995
PsiI TTATAA 1 cut(s) 4466
Psp124BI GAGCTC 7 cut(s) 911, 2200, 3101, 3119, 3247, 4054, 4361
PspCI CACGTG 1 cut(s) 1610
PspEI GGTNACC 1 cut(s) 3047
PspOMI GGGCCC 1 cut(s) 3670
PstI CTGCAG 2 cut(s) 2468, 4021
PstNI CAGNNNCTG 3 cut(s) 3109, 3350, 4535
PsuI RGATCY 9 cut(s) 831, 2122, 2260, 2774, 2837, 3106, 3974, 4241, 4573
PsyI GACNNNGTC 2 cut(s) 219, 4063
PvuII CAGCTG 4 cut(s) 212, 485, 867, 1412
RruI TCGCGA 1 cut(s) 1491
RseI CAYNNNNRTG 5 cut(s) 126, 660, 882, 1353, 2007
SacI GAGCTC 7 cut(s) 911, 2200, 3101, 3119, 3247, 4054, 4361
SapI GCTCTTC 1 cut(s) 42
SchI GAGTC 8 cut(s) 1114, 1316, 1678, 2006, 3082, 3878, 4606, 5183
SfcI CTRYAG 5 cut(s) 1389, 2464, 3705, 4017, 5267
SinI GGWCC 7 cut(s) 215, 1175, 1312, 1747, 3237, 4065, 4108
SmiMI CAYNNNNRTG 5 cut(s) 126, 660, 882, 1353, 2007
SmlI CTYRAG 4 cut(s) 85, 1113, 1183, 4118
SmoI CTYRAG 4 cut(s) 85, 1113, 1183, 4118
SnaBI TACGTA 1 cut(s) 1369
SpeI ACTAGT 2 cut(s) 3449, 3995
SphI GCATGC 2 cut(s) 313, 1873
SseBI AGGCCT 1 cut(s) 1042
SspI AATATT 3 cut(s) 777, 2395, 2824
SstI GAGCTC 7 cut(s) 911, 2200, 3101, 3119, 3247, 4054, 4361
StuI AGGCCT 1 cut(s) 1042
StyI CCWWGG 8 cut(s) 1990, 2337, 3554, 4068, 4142, 4454, 4805, 4865
TaiI ACGT 5 cut(s) 1371, 1612, 1940, 2558, 4817
TaqI TCGA 9 cut(s) 754, 2695, 2892, 3247, 3582, 3638, 3665, 3867, 5098
TaqII GACCGA 1 cut(s) 2324
TatI WGTACW 6 cut(s) 235, 434, 1192, 2171, 2388, 4827
TauI GCSGC 2 cut(s) 3262, 3834
TspGWI ACGGA 4 cut(s) 1929, 1947, 2850, 5209
Tth111I GACNNNGTC 2 cut(s) 219, 4063
Van91I CCANNNNNTGG 2 cut(s) 2343, 4535
VneI GTGCAC 1 cut(s) 663
VpaK11BI GGWCC 7 cut(s) 215, 1175, 1312, 1747, 3237, 4065, 4108
VspI ATTAAT 1 cut(s) 4995
XagI CCTNNNNNAGG 1 cut(s) 3584
XbaI TCTAGA 3 cut(s) 1397, 2257, 5215
XceI RCATGY 5 cut(s) 313, 583, 1873, 3145, 4282
XcmI CCANNNNNNNNNTGG 3 cut(s) 919, 1757, 4803
XmaJI CCTAGG 1 cut(s) 4142
XmiI GTMKAC 1 cut(s) 4148
XmnI GAANNNNTTC 2 cut(s) 1921, 2653
ZraI GACGTC 1 cut(s) 2556
Zsp2I ATGCAT 5 cut(s) 201, 292, 315, 1732, 4284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.