MD13G1095900.v1.1

Involved in the biosynthesis of phylloquinone (vitamin K1). Methyltransferase required for the conversion of 2-phytyl- 1,4-beta-naphthoquinol to phylloquinol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003403
Physical Location & Seq
Forward (+)
6769503 .. 6771726
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1095900.v1.1.491

Sequence Viewer

Length: 891 bp
ATGAGTACTGATGTGATTTTGATTGTTGACCTTCTAAGTGTTTATGAACACATAGAAATTAAACAAAGTAACGCGCGTCTACCCGTTGTTGTTTCAGTAACTGACGCTAACTGTCATGGCTTCGCTCTTCCGTTCTCAGCCGGTCAGCGCCTACCCAGTTTTCGGTTCAGACGCAGTCCGGTCCGGTCCTCCAGGACCTCCACCGCACGCCAGGCGCTCTTCAACCGCATTGCCCCTGTCTATGATAACCTAAATGATTTGTTGAGCTTGGGTCAGCATCGAATATGGAAAAGAATGGCAGTTTTGTGGAGCGGAGCAAAAATGGGAGCGTTTGTGTTGGATTTTTGCAGTGGAAGTGGGGATTTAGCGTTTCTCTTATCTGAGAAAGTTGGCTCCAATGGCAAGGTGATTGGTCTCGATTTCTTGAAAGAGCAGTTGTCAATTGCTTCGTCGCGGCAAAAGTTGAAGTCGAAAGCCTGCTGCTTGAACATTGAGTGGGTTGAAGGTGATGCAACTGATTTGCCGTTTTCTGACCGTCACTTTGATGCCATCACTATGGGTTATGGGCTAAGAAATGTAGTAGACAAATATAAGGCCATGGAGGAAATGTTTAGAGTTTTGAAAGCAGGCTCGAGGGTATCAATCCTTGATTTCAATAAAAGCACCAATCCAGTTGTGTCTGCAGTTCAGGAATTGATGATTGACAATCTTGTTGTCCCAGTGGCTTCTGGTTTTGGACTTGAGGAGGATTACAAGTATTTAAAGAGCTCAGTCATGGCATTCCTGCCAGGGGACGAGTTGGAGAACCTGGCTTTAGACGTAGGTTTTTCCAATGCCAGATATTATGAGATTGGCAGAAGCCTCATTGGGAACTTAGTAGCCACACGATAG

Protein Analysis

297

Amino Acids

32.8

Weight (kDa)

8.28

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 70 - 289 4.8e-55 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 109 - 278 2.3e-07 Methyltransferase domain
Methyltransf_31 PF13847 110 - 223 1.4e-19 Methyltransferase domain
Methyltransf_25 PF13649 112 - 210 9.9e-18 Methyltransferase domain
Methyltransf_11 PF08241 113 - 213 7.2e-16 Methyltransferase domain
Methyltransf_12 PF08242 113 - 212 7e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 2 cut(s) 79, 582
AccII CGCG 3 cut(s) 74, 76, 454
AciI CCGC 4 cut(s) 204, 226, 312, 454
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 162, 790
AgsI TTSAA 7 cut(s) 223, 427, 466, 487, 503, 622, 655
AjnI CCWGG 4 cut(s) 191, 210, 787, 807
AluBI AGCT 2 cut(s) 267, 768
AluI AGCT 2 cut(s) 267, 768
Alw21I GWGCWC 1 cut(s) 770
Alw26I GTCTC 1 cut(s) 419
AlwNI CAGNNNCTG 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 1 cut(s) 480
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
AspLEI GCGC 3 cut(s) 76, 150, 217
AspS9I GGNCC 3 cut(s) 181, 186, 195
AsuHPI GGTGA 2 cut(s) 418, 518
AvaI CYCGRG 1 cut(s) 631
AvaII GGWCC 3 cut(s) 181, 186, 195
BanII GRGCYC 1 cut(s) 770
Bbv12I GWGCWC 1 cut(s) 770
BbvI GCAGC 1 cut(s) 467
BccI CCATC 1 cut(s) 557
BceAI ACGGC 1 cut(s) 508
BciT130I CCWGG 4 cut(s) 193, 212, 789, 809
BcoDI GTCTC 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 681
BfoI RGCGCY 2 cut(s) 151, 218
BisI GCNGC 2 cut(s) 455, 481
BlsI GCNGC 2 cut(s) 456, 482
BmcAI AGTACT 1 cut(s) 7
Bme1390I CCNGG 4 cut(s) 193, 212, 789, 809
Bme18I GGWCC 3 cut(s) 181, 186, 195
BmeT110I CYCGRG 1 cut(s) 631
BmgT120I GGNCC 3 cut(s) 181, 186, 195
BmiI GGNNCC 1 cut(s) 394
BmrFI CCNGG 4 cut(s) 193, 212, 789, 809
BmrI ACTGGG 2 cut(s) 150, 713
BmsI GCATC 3 cut(s) 286, 499, 535
BmuI ACTGGG 2 cut(s) 150, 713
BpmI CTGGAG 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 761
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 597, 788
BsaWI WCCGGW 2 cut(s) 178, 183
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 593, 623
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 790
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 3 cut(s) 156, 671, 719
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 4 cut(s) 193, 212, 789, 809
BseDI CCNNGG 2 cut(s) 597, 788
BseLI CCNNNNNNNGG 2 cut(s) 162, 790
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 3 cut(s) 150, 372, 783
BseNI ACTGG 3 cut(s) 156, 671, 719
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 1 cut(s) 467
Bsh1236I CGCG 3 cut(s) 74, 76, 454
BshFI GGCC 1 cut(s) 596
BsiHKAI GWGCWC 1 cut(s) 770
BsiHKCI CYCGRG 1 cut(s) 631
BsiSI CCGG 3 cut(s) 141, 179, 184
BslFI GGGAC 2 cut(s) 701, 806
BslI CCNNNNNNNGG 2 cut(s) 162, 790
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 2 cut(s) 701, 806
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 1 cut(s) 596
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 770
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 4 cut(s) 204, 226, 312, 454
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 3 cut(s) 149, 373, 782
BspFNI CGCG 3 cut(s) 74, 76, 454
BspLI GGNNCC 1 cut(s) 394
BspMAI CTGCAG 1 cut(s) 685
BspQI GCTCTTC 2 cut(s) 132, 224
BspTNI GGTCTC 1 cut(s) 419
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 3 cut(s) 156, 671, 719
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 597, 788
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 4 cut(s) 193, 212, 789, 809
Bst4CI ACNGT 2 cut(s) 113, 536
Bst6I CTCTTC 2 cut(s) 132, 224
BstC8I GCNNGC 3 cut(s) 208, 478, 628
BstDEI CTNAG 6 cut(s) 35, 136, 381, 569, 769, 874
BstDSI CCRYGG 1 cut(s) 597
BstFNI CGCG 3 cut(s) 74, 76, 454
BstH2I RGCGCY 2 cut(s) 151, 218
BstHHI GCGC 3 cut(s) 76, 150, 217
BstMAI GTCTC 1 cut(s) 419
BstMWI GCNNNNNNNGC 2 cut(s) 212, 399
BstNI CCWGG 4 cut(s) 193, 212, 789, 809
BstSCI CCNGG 4 cut(s) 191, 210, 787, 807
BstSFI CTRYAG 1 cut(s) 681
BstUI CGCG 3 cut(s) 74, 76, 454
BstV1I GCAGC 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 556
BsuRI GGCC 1 cut(s) 596
BtgI CCRYGG 1 cut(s) 597
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 2 cut(s) 355, 726
Cac8I GCNNGC 3 cut(s) 208, 478, 628
CaiI CAGNNNCTG 1 cut(s) 101
CfoI GCGC 3 cut(s) 76, 150, 217
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 181, 186, 195
CpoI CGGWCCG 1 cut(s) 181
CseI GACGC 3 cut(s) 65, 113, 180
Csp6I GTAC 1 cut(s) 6
CspI CGGWCCG 1 cut(s) 181
CviAII CATG 3 cut(s) 116, 598, 775
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 6 cut(s) 35, 136, 381, 569, 769, 874
DraI TTTAAA 1 cut(s) 762
Eam1104I CTCTTC 2 cut(s) 132, 224
EarI CTCTTC 2 cut(s) 132, 224
Ecl136II GAGCTC 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 770
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 3 cut(s) 181, 186, 195
Eco53kI GAGCTC 1 cut(s) 768
Eco88I CYCGRG 1 cut(s) 631
EcoICRI GAGCTC 1 cut(s) 768
EcoO109I RGGNCCY 1 cut(s) 195
EcoRII CCWGG 4 cut(s) 191, 210, 787, 807
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 770
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 3 cut(s) 119, 601, 778
FaqI GGGAC 2 cut(s) 701, 806
FatI CATG 3 cut(s) 115, 597, 774
FblI GTMKAC 2 cut(s) 79, 582
Fnu4HI GCNGC 2 cut(s) 455, 481
FriOI GRGCYC 1 cut(s) 770
Fsp4HI GCNGC 2 cut(s) 455, 481
GlaI GCGC 3 cut(s) 75, 149, 216
GluI GCNGC 2 cut(s) 455, 481
GsuI CTGGAG 1 cut(s) 175
HaeII RGCGCY 2 cut(s) 151, 218
HaeIII GGCC 1 cut(s) 596
HapII CCGG 3 cut(s) 141, 179, 184
HgaI GACGC 3 cut(s) 65, 113, 180
HhaI GCGC 3 cut(s) 76, 150, 217
Hin1II CATG 3 cut(s) 119, 601, 778
Hin6I GCGC 3 cut(s) 74, 148, 215
HinP1I GCGC 3 cut(s) 74, 148, 215
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HpaII CCGG 3 cut(s) 141, 179, 184
HphI GGTGA 2 cut(s) 418, 518
Hpy166II GTNNAC 3 cut(s) 28, 80, 583
Hpy188I TCNGA 3 cut(s) 170, 382, 532
Hpy188III TCNNGA 3 cut(s) 416, 424, 689
Hpy8I GTNNAC 3 cut(s) 28, 80, 583
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 2 cut(s) 41, 497
HpyCH4III ACNGT 2 cut(s) 113, 536
HpyCH4IV ACGT 1 cut(s) 819
HpyCH4V TGCA 3 cut(s) 348, 512, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 399
HpyF3I CTNAG 6 cut(s) 35, 136, 381, 569, 769, 874
HpySE526I ACGT 1 cut(s) 819
Hsp92II CATG 3 cut(s) 119, 601, 778
HspAI GCGC 3 cut(s) 74, 148, 215
LguI GCTCTTC 2 cut(s) 132, 224
LmnI GCTCC 4 cut(s) 309, 314, 326, 398
Lsp1109I GCAGC 1 cut(s) 467
LweI GCATC 3 cut(s) 286, 499, 535
MaeII ACGT 1 cut(s) 819
MaeIII GTNAC 3 cut(s) 68, 97, 536
MbiI CCGCTC 1 cut(s) 312
MboII GAAGA 2 cut(s) 119, 211
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 1 cut(s) 770
MluCI AATT 3 cut(s) 57, 441, 692
MmeI TCCRAC 2 cut(s) 318, 780
MnlI CCTC 7 cut(s) 199, 208, 595, 627, 736, 739, 872
MseI TTAA 2 cut(s) 60, 761
MslI CAYNNNNRTG 2 cut(s) 543, 554
MspI CCGG 3 cut(s) 141, 179, 184
MspR9I CCNGG 4 cut(s) 193, 212, 789, 809
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 779
MvaI CCWGG 4 cut(s) 193, 212, 789, 809
MvnI CGCG 3 cut(s) 74, 76, 454
MwoI GCNNNNNNNGC 2 cut(s) 212, 399
NcoI CCATGG 1 cut(s) 597
NlaIII CATG 3 cut(s) 119, 601, 778
NlaIV GGNNCC 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 536
PaeR7I CTCGAG 1 cut(s) 631
PciSI GCTCTTC 2 cut(s) 132, 224
PctI GAATGC 1 cut(s) 779
PflFI GACNNNGTC 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 2 cut(s) 456, 482
PpuMI RGGWCCY 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 770
Psp5II RGGWCCY 1 cut(s) 195
Psp6I CCWGG 4 cut(s) 191, 210, 787, 807
PspGI CCWGG 4 cut(s) 191, 210, 787, 807
PspN4I GGNNCC 1 cut(s) 394
PspPI GGNCC 3 cut(s) 181, 186, 195
PspPPI RGGWCCY 1 cut(s) 195
PspXI VCTCGAGB 1 cut(s) 631
PstI CTGCAG 1 cut(s) 685
PstNI CAGNNNCTG 1 cut(s) 101
PsyI GACNNNGTC 1 cut(s) 174
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 2 cut(s) 543, 554
Rsr2I CGGWCCG 1 cut(s) 181
RsrII CGGWCCG 1 cut(s) 181
SacI GAGCTC 1 cut(s) 770
SapI GCTCTTC 2 cut(s) 132, 224
SaqAI TTAA 2 cut(s) 60, 761
SatI GCNGC 2 cut(s) 455, 481
Sau96I GGNCC 3 cut(s) 181, 186, 195
ScaI AGTACT 1 cut(s) 7
ScrFI CCNGG 4 cut(s) 193, 212, 789, 809
SduI GDGCHC 1 cut(s) 770
SfaNI GCATC 3 cut(s) 286, 499, 535
SfcI CTRYAG 1 cut(s) 681
Sfr274I CTCGAG 1 cut(s) 631
SinI GGWCC 3 cut(s) 181, 186, 195
SlaI CTCGAG 1 cut(s) 631
SmiMI CAYNNNNRTG 2 cut(s) 543, 554
SmlI CTYRAG 2 cut(s) 631, 740
SmoI CTYRAG 2 cut(s) 631, 740
Sse9I AATT 3 cut(s) 57, 441, 692
SsiI CCGC 4 cut(s) 204, 226, 312, 454
SstI GAGCTC 1 cut(s) 770
StyD4I CCNGG 4 cut(s) 191, 210, 787, 807
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 2 cut(s) 113, 536
TaiI ACGT 1 cut(s) 822
TaqI TCGA 4 cut(s) 280, 417, 470, 632
TasI AATT 3 cut(s) 57, 441, 692
TatI WGTACW 1 cut(s) 5
TauI GCSGC 1 cut(s) 457
Tru1I TTAA 2 cut(s) 60, 761
Tru9I TTAA 2 cut(s) 60, 761
TscAI CASTG 2 cut(s) 355, 726
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 1 cut(s) 480
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 1 cut(s) 60
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 2 cut(s) 355, 726
Tth111I GACNNNGTC 1 cut(s) 174
VpaK11BI GGWCC 3 cut(s) 181, 186, 195
XhoI CTCGAG 1 cut(s) 631
XmiI GTMKAC 2 cut(s) 79, 582
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.