Rw4G028090

Involved in the biosynthesis of phylloquinone (vitamin K1). Methyltransferase required for the conversion of 2-phytyl- 1,4-beta-naphthoquinol to phylloquinol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003403
Physical Location & Seq
Reverse (-)
54767923 .. 54769493
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028090.1

Sequence Viewer

Length: 777 bp
ATGGCTTCGCTCCAGCTTTACCTTCCATTCTCCACCGGTCCATCTCTACCCAATTTCCGGTTCAGACGCACTCCGGTCCGATCCTCCAGCGAGCGGCAGGCTCTCTTCAACCGCATTGCCCCTGTCTACGACAACTTAAATGATTTGCTGAGCTTGGGTCAGCACAGAATCTGGAAAAGAATGGCTGTGTCATGGAGTGGTGCAAAAATGGGTGATAGTGTATTGGATTTGTGCTGTGGAAGTGGGGATATAGCGTTTCTGTTGTCTGAGAAAGTTGAGGTCAATGGCAAGGTGATTGGTTTGGATTTTTCAAAAAAGCAGTTATCGGTTGCGTCTTCCCGGCAGAAGGTGAAGTCCAAAGCCTGCTACATGAACATTGTGTGGGTTGAAGGTGATGCAGTTGATTTGCCATTTTCTAATGGTCAATTTGATGCTATAACTATGGGTTATGGGCTGCGAAATGTAGTAGACAAACATAAGGCAATGCAGGAAATTTTTAGAGTTTTGAAAGCAGGCTCAAGAGTGTCTATCCTTGATTTTAATAAGAGTACCAACCCAGTAACTGCCTCATTTCAGGAAATGATGATTGATAATGTTGTTGTCCCTGTGGCTTCTGGTTTTGGCCTTGAAGAGGATTACAAATACTTAAGGAGATCAATCAGGGAATTTTTGACAGGGGAGGAGTTGGAGATGCTCGCTTTAGATGTAGGTTTTTCTGATGCAAGATATTATGAGATTGGTGGAGGCCTTATGGGGAACTTAGTAGCCACCCGCTAA

Protein Analysis

258

Amino Acids

28.73

Weight (kDa)

8.69

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 33 - 252 1.1e-59 ubiE/COQ5 methyltransferase family
PCMT PF01135 62 - 155 7e-06 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_23 PF13489 69 - 238 1.2e-09 Methyltransferase domain
Methyltransf_31 PF13847 71 - 186 1.8e-21 Methyltransferase domain
Methyltransf_25 PF13649 74 - 172 3e-19 Methyltransferase domain
Methyltransf_11 PF08241 75 - 175 1.1e-17 Methyltransferase domain
Methyltransf_12 PF08242 75 - 174 6.9e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 94
AccI GTMKAC 2 cut(s) 126, 468
AciI CCGC 3 cut(s) 94, 112, 772
AclWI GGATC 1 cut(s) 75
AcsI RAATTY 2 cut(s) 492, 665
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 4 cut(s) 57, 93, 346, 631
AflII CTTAAG 1 cut(s) 646
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 5 cut(s) 109, 312, 389, 508, 629
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 2 cut(s) 16, 153
AluI AGCT 2 cut(s) 16, 153
AlwI GGATC 1 cut(s) 75
AlwNI CAGNNNCTG 2 cut(s) 171, 563
AoxI GGCC 2 cut(s) 622, 745
ApeKI GCWGC 1 cut(s) 454
ApoI RAATTY 2 cut(s) 492, 665
AsiGI ACCGGT 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 38, 76
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 4 cut(s) 224, 304, 361, 404
AvaII GGWCC 2 cut(s) 38, 76
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 441
BccI CCATC 1 cut(s) 49
BcnI CCSGG 1 cut(s) 340
BfrI CTTAAG 1 cut(s) 646
BisI GCNGC 2 cut(s) 95, 455
BlpI GCTNAGC 1 cut(s) 149
BlsI GCNGC 2 cut(s) 96, 456
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 2 cut(s) 38, 76
BmgT120I GGNCC 2 cut(s) 38, 76
BmrFI CCNGG 1 cut(s) 340
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 4 cut(s) 385, 421, 681, 709
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 70
Bpu1102I GCTNAGC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 502
BpuMI CCSGG 1 cut(s) 340
BsaWI WCCGGW 3 cut(s) 35, 57, 73
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 93, 346, 631
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 557
Bse3DI GCAATG 2 cut(s) 114, 489
BseLI CCNNNNNNNGG 4 cut(s) 57, 93, 346, 631
BseMI GCAATG 2 cut(s) 114, 489
BseMII CTCAG 2 cut(s) 140, 258
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 695
BseXI GCAGC 1 cut(s) 441
BshFI GGCC 2 cut(s) 624, 747
BshTI ACCGGT 1 cut(s) 35
BsiSI CCGG 4 cut(s) 36, 58, 74, 340
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 4 cut(s) 57, 93, 346, 631
BsmFI GGGAC 1 cut(s) 587
BsnI GGCC 2 cut(s) 624, 747
Bsp143I GATC 2 cut(s) 80, 653
Bsp1720I GCTNAGC 1 cut(s) 149
BspACI CCGC 3 cut(s) 94, 112, 772
BspANI GGCC 2 cut(s) 624, 747
BspCNI CTCAG 2 cut(s) 141, 259
BspPI GGATC 1 cut(s) 75
BspTI CTTAAG 1 cut(s) 646
BsrBI CCGCTC 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 114, 489
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 35
BssMI GATC 2 cut(s) 80, 653
Bst6I CTCTTC 2 cut(s) 110, 624
BstAFI CTTAAG 1 cut(s) 646
BstC8I GCNNGC 5 cut(s) 92, 99, 364, 514, 696
BstDEI CTNAG 3 cut(s) 149, 267, 760
BstENI CCTNNNNNAGG 1 cut(s) 629
BstKTI GATC 2 cut(s) 83, 656
BstMBI GATC 2 cut(s) 80, 653
BstSCI CCNGG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 441
BstV2I GAAGAC 1 cut(s) 327
BsuRI GGCC 2 cut(s) 624, 747
Cac8I GCNNGC 5 cut(s) 92, 99, 364, 514, 696
CaiI CAGNNNCTG 2 cut(s) 171, 563
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 38, 76
CpoI CGGWCCG 1 cut(s) 76
CseI GACGC 2 cut(s) 75, 321
Csp6I GTAC 1 cut(s) 549
CspAI ACCGGT 1 cut(s) 35
CspI CGGWCCG 1 cut(s) 76
CviAII CATG 2 cut(s) 192, 370
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 3 cut(s) 149, 267, 760
DpnI GATC 2 cut(s) 82, 655
DpnII GATC 2 cut(s) 80, 653
Eam1104I CTCTTC 2 cut(s) 110, 624
EarI CTCTTC 2 cut(s) 110, 624
Eco147I AGGCCT 1 cut(s) 747
Eco47I GGWCC 2 cut(s) 38, 76
EcoNI CCTNNNNNAGG 1 cut(s) 629
FaeI CATG 2 cut(s) 195, 373
FaiI YATR 9 cut(s) 193, 251, 371, 437, 443, 450, 477, 732, 752
FaqI GGGAC 1 cut(s) 587
FatI CATG 2 cut(s) 191, 369
FblI GTMKAC 2 cut(s) 126, 468
Fnu4HI GCNGC 2 cut(s) 95, 455
Fsp4HI GCNGC 2 cut(s) 95, 455
GluI GCNGC 2 cut(s) 95, 455
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 2 cut(s) 624, 747
HapII CCGG 4 cut(s) 36, 58, 74, 340
HgaI GACGC 2 cut(s) 75, 321
Hin1II CATG 2 cut(s) 195, 373
HinfI GANTC 1 cut(s) 168
HpaII CCGG 4 cut(s) 36, 58, 74, 340
HphI GGTGA 4 cut(s) 224, 304, 361, 404
Hpy166II GTNNAC 2 cut(s) 127, 469
Hpy188I TCNGA 4 cut(s) 65, 80, 268, 718
Hpy188III TCNNGA 3 cut(s) 172, 519, 575
Hpy8I GTNNAC 2 cut(s) 127, 469
HpyAV CCTTC 3 cut(s) 32, 340, 383
HpyCH4V TGCA 4 cut(s) 203, 398, 487, 722
HpyF3I CTNAG 3 cut(s) 149, 267, 760
Hsp92II CATG 2 cut(s) 195, 373
Kzo9I GATC 2 cut(s) 80, 653
LmnI GCTCC 1 cut(s) 15
Lsp1109I GCAGC 1 cut(s) 441
LweI GCATC 4 cut(s) 385, 421, 681, 709
MaeIII GTNAC 1 cut(s) 559
MalI GATC 2 cut(s) 82, 655
MbiI CCGCTC 1 cut(s) 94
MboI GATC 2 cut(s) 80, 653
MboII GAAGA 3 cut(s) 97, 327, 641
MluCI AATT 4 cut(s) 52, 425, 492, 665
MmeI TCCRAC 1 cut(s) 666
MnlI CCTC 6 cut(s) 94, 271, 577, 625, 673, 737
MseI TTAA 3 cut(s) 137, 540, 647
MspCI CTTAAG 1 cut(s) 646
MspI CCGG 4 cut(s) 36, 58, 74, 340
MspR9I CCNGG 1 cut(s) 340
NciI CCSGG 1 cut(s) 340
NdeII GATC 2 cut(s) 80, 653
NlaIII CATG 2 cut(s) 195, 373
PceI AGGCCT 1 cut(s) 747
PfeI GAWTC 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 2 cut(s) 96, 456
PspPI GGNCC 2 cut(s) 38, 76
PstNI CAGNNNCTG 2 cut(s) 171, 563
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
Rsr2I CGGWCCG 1 cut(s) 76
RsrII CGGWCCG 1 cut(s) 76
SaqAI TTAA 3 cut(s) 137, 540, 647
SatI GCNGC 2 cut(s) 95, 455
Sau3AI GATC 2 cut(s) 80, 653
Sau96I GGNCC 2 cut(s) 38, 76
ScrFI CCNGG 1 cut(s) 340
SetI ASST 8 cut(s) 18, 24, 155, 282, 294, 351, 394, 712
SfaNI GCATC 4 cut(s) 385, 421, 681, 709
SinI GGWCC 2 cut(s) 38, 76
SmlI CTYRAG 2 cut(s) 517, 646
SmoI CTYRAG 2 cut(s) 517, 646
Sse9I AATT 4 cut(s) 52, 425, 492, 665
SseBI AGGCCT 1 cut(s) 747
SsiI CCGC 3 cut(s) 94, 112, 772
StuI AGGCCT 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 338
TasI AATT 4 cut(s) 52, 425, 492, 665
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 1 cut(s) 168
Tru1I TTAA 3 cut(s) 137, 540, 647
Tru9I TTAA 3 cut(s) 137, 540, 647
TseI GCWGC 1 cut(s) 454
TspDTI ATGAA 1 cut(s) 386
Vha464I CTTAAG 1 cut(s) 646
VpaK11BI GGWCC 2 cut(s) 38, 76
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 2 cut(s) 492, 665
XmiI GTMKAC 2 cut(s) 126, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.