Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003403

Physical Location & Seq

Genomic Coordinates Forward (+)

6808459 .. 6810375

Gene Structure 1917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1096300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 546 bp

ATGGCAGTTTCGTTGAGCGGAGCAAAAATGGGAGAGAGTGTGTTGGATTTGTGCTGTGGAAGTGGGGATTTAGCGTTTCTCTTGTCTGAGAAAGTTGGGTGTAATGGCAAGGTGATTGGTCTCGATTTCTCGAAAGAGCAGTTGTCAGTTGCTTCGTCGCGGCAAAAGTTGAAGTCGAAAGCCTGCTACTTGAACATTGAGTGGGTTGAAGGTGATGCAACTGATTTGCCGTTTTCTGACGTTCACTTTGATGCCATCACTATGGGTTTTGGGCTAAAGAAATGCTCGAGGGTATCGATCCTTGATTTCAATAAAGGCATCAATCCAATTGTTTCATCAGTTCAGGAATTGATGATTGACAATGTTGTTGTCCCGGTGGCTTCTGATTTTGGACTTGAGGAGGATTACAAGTATTTAAAGAGTTCAATCAGGGAATTTCTGACAGGGGACGAGTTGGAGAAGCTGGCTTTGGACGTAGGTTTTTCCAGTGCCACATATTATGAGATTGGGGGAAGCCTCATGGGGAACTTAGTAGCCATACGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0008168 GO:0008169 GO:0008171 GO:0009058 GO:0009108 GO:0009110 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016741 GO:0032259 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051188 GO:0052624 GO:0071704 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

Ubie_methyltran

Protein Analysis

182

Amino Acids

19.54

Weight (kDa)

4.67

Isoelectric Point (pI)

31.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubie_methyltran	PF01209	4 - 95	7.1e-23	ubiE/COQ5 methyltransferase family
PCMT	PF01135	8 - 96	2e-06	Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_31	PF13847	10 - 87	1.8e-15	Methyltransferase domain
Methyltransf_25	PF13649	14 - 93	1.1e-16	Methyltransferase domain
Methyltransf_11	PF08241	15 - 95	8.4e-12	Methyltransferase domain
Methyltransf_12	PF08242	15 - 90	1.6e-08	Methyltransferase domain
Ubie_methyltran	PF01209	92 - 174	3.5e-07	ubiE/COQ5 methyltransferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0003403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23360 AT1G23360 AT1G23360
fragaria_vesca	FvH4_4g26051 FvH4_4g26060 FvH4_4g26080
malus_domestica	MD13G1095900.v1.1 MD13G1096300.v1.1 MD16G1097300.v1.1
prunus_persica	Prupe.1G249500_v2.0.a1
pyrus_communis	pycom13g08360 pycom13g08390 pycom16g08290
rosa_chinensis	RchiOBHm_Chr2g0132031 RchiOBHm_Chr4g0433541 RchiOBHm_Chr6g0273261
rosa_laevigata	RLG00000002005 RLG00000006744 RLG00000033410
rosa_multiflora	Rmu_sc0000312.1_g000010 Rmu_sc0009599.1_g000015
rosa_roxburghii	Rroxscaffold_3G00246010 Rroxscaffold_4G00300010
rosa_rugosa	Rorug04G0269700
rosa_samantha	Rh2BG361000 Rh4AG164200 Rh4AG324400 Rh4BG332300 Rh4CG347600 Rh4DG328600 Rh5AG111900 Rh5AG129600 Rh6AG348700
rosa_wichuraiana	Rw0G020180 Rw4G028090 Rw6G041620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	18
AccII	CGCG	1 cut(s)	160
AciI	CCGC	2 cut(s)	18, 160
AclWI	GGATC	1 cut(s)	292
AcsI	RAATTY	1 cut(s)	434
AgsI	TTSAA	5 cut(s)	172, 193, 209, 310, 426
AjuI	GAANNNNNNNTTGG	2 cut(s)	452, 484
AluBI	AGCT	1 cut(s)	463
AluI	AGCT	1 cut(s)	463
Alw26I	GTCTC	1 cut(s)	125
AlwI	GGATC	1 cut(s)	292
Ama87I	CYCGRG	1 cut(s)	286
ApoI	RAATTY	1 cut(s)	434
ArsI	GACNNNNNNTTYG	2 cut(s)	230, 262
AsuC2I	CCSGG	1 cut(s)	374
AsuHPI	GGTGA	2 cut(s)	124, 224
AvaI	CYCGRG	1 cut(s)	286
BccI	CCATC	1 cut(s)	263
BceAI	ACGGC	1 cut(s)	214
BcnI	CCSGG	1 cut(s)	374
BcoDI	GTCTC	1 cut(s)	125
BisI	GCNGC	1 cut(s)	161
BlsI	GCNGC	1 cut(s)	162
Bme1390I	CCNGG	1 cut(s)	374
BmeT110I	CYCGRG	1 cut(s)	286
BmrFI	CCNGG	1 cut(s)	374
BmsI	GCATC	3 cut(s)	205, 241, 327
BpuEI	CTTGAG	1 cut(s)	416
BpuMI	CCSGG	1 cut(s)	374
Bsa29I	ATCGAT	1 cut(s)	296
BsaI	GGTCTC	1 cut(s)	125
BsaXI	ACNNNNNCTCC	2 cut(s)	24, 54
Bse1I	ACTGG	1 cut(s)	486
BseCI	ATCGAT	1 cut(s)	296
BseMII	CTCAG	1 cut(s)	78
BseNI	ACTGG	1 cut(s)	486
BseRI	GAGGAG	1 cut(s)	413
Bsh1236I	CGCG	1 cut(s)	160
BshVI	ATCGAT	1 cut(s)	296
BsiHKCI	CYCGRG	1 cut(s)	286
BsiSI	CCGG	1 cut(s)	374
BslFI	GGGAC	2 cut(s)	356, 461
BsmAI	GTCTC	1 cut(s)	125
BsmFI	GGGAC	2 cut(s)	356, 461
Bso31I	GGTCTC	1 cut(s)	125
BsoBI	CYCGRG	1 cut(s)	286
Bsp143I	GATC	1 cut(s)	297
BspACI	CCGC	2 cut(s)	18, 160
BspCNI	CTCAG	1 cut(s)	79
BspDI	ATCGAT	1 cut(s)	296
BspFNI	CGCG	1 cut(s)	160
BspPI	GGATC	1 cut(s)	292
BspTNI	GGTCTC	1 cut(s)	125
BsrBI	CCGCTC	1 cut(s)	18
BsrI	ACTGG	1 cut(s)	486
BssMI	GATC	1 cut(s)	297
BstC8I	GCNNGC	2 cut(s)	184, 465
BstDEI	CTNAG	2 cut(s)	87, 529
BstFNI	CGCG	1 cut(s)	160
BstKTI	GATC	1 cut(s)	300
BstMAI	GTCTC	1 cut(s)	125
BstMBI	GATC	1 cut(s)	297
BstSCI	CCNGG	1 cut(s)	372
BstUI	CGCG	1 cut(s)	160
BstXI	CCANNNNNNTGG	1 cut(s)	262
Bsu15I	ATCGAT	1 cut(s)	296
BsuTUI	ATCGAT	1 cut(s)	296
BtsIMutI	CAGTG	1 cut(s)	493
Cac8I	GCNNGC	2 cut(s)	184, 465
ClaI	ATCGAT	1 cut(s)	296
CviAII	CATG	1 cut(s)	520
CviJI	RGCY	7 cut(s)	182, 274, 380, 463, 467, 516, 536
CviKI_1	RGCY	7 cut(s)	182, 274, 380, 463, 467, 516, 536
DdeI	CTNAG	2 cut(s)	87, 529
DpnI	GATC	1 cut(s)	299
DpnII	GATC	1 cut(s)	297
DraI	TTTAAA	1 cut(s)	417
Eco31I	GGTCTC	1 cut(s)	125
Eco88I	CYCGRG	1 cut(s)	286
FaeI	CATG	1 cut(s)	523
FaiI	YATR	5 cut(s)	263, 496, 501, 521, 539
FaqI	GGGAC	2 cut(s)	356, 461
FatI	CATG	1 cut(s)	519
Fnu4HI	GCNGC	1 cut(s)	161
Fsp4HI	GCNGC	1 cut(s)	161
GluI	GCNGC	1 cut(s)	161
HapII	CCGG	1 cut(s)	374
Hin1II	CATG	1 cut(s)	523
HpaII	CCGG	1 cut(s)	374
HphI	GGTGA	2 cut(s)	124, 224
Hpy166II	GTNNAC	1 cut(s)	244
Hpy188I	TCNGA	4 cut(s)	88, 238, 385, 441
Hpy188III	TCNNGA	3 cut(s)	122, 130, 344
Hpy8I	GTNNAC	1 cut(s)	244
Hpy99I	CGWCG	1 cut(s)	160
HpyAV	CCTTC	1 cut(s)	203
HpyCH4IV	ACGT	2 cut(s)	240, 474
HpyCH4V	TGCA	1 cut(s)	218
HpyF3I	CTNAG	2 cut(s)	87, 529
HpySE526I	ACGT	2 cut(s)	240, 474
Hsp92II	CATG	1 cut(s)	523
Kzo9I	GATC	1 cut(s)	297
LmnI	GCTCC	1 cut(s)	20
LpnPI	CCDG	7 cut(s)	196, 329, 387, 415, 429, 449, 499
LweI	GCATC	3 cut(s)	205, 241, 327
MaeII	ACGT	2 cut(s)	240, 474
MalI	GATC	1 cut(s)	299
MbiI	CCGCTC	1 cut(s)	18
MboI	GATC	1 cut(s)	297
MfeI	CAATTG	1 cut(s)	327
MluCI	AATT	3 cut(s)	327, 347, 434
MmeI	TCCRAC	2 cut(s)	24, 435
MnlI	CCTC	4 cut(s)	282, 391, 394, 527
MseI	TTAA	1 cut(s)	416
MslI	CAYNNNNRTG	2 cut(s)	249, 260
MspI	CCGG	1 cut(s)	374
MspR9I	CCNGG	1 cut(s)	374
MunI	CAATTG	1 cut(s)	327
MvnI	CGCG	1 cut(s)	160
NciI	CCSGG	1 cut(s)	374
NdeII	GATC	1 cut(s)	297
NlaIII	CATG	1 cut(s)	523
PaeR7I	CTCGAG	1 cut(s)	286
PcsI	WCGNNNNNNNCGW	1 cut(s)	293
PkrI	GCNGC	1 cut(s)	162
PspXI	VCTCGAGB	1 cut(s)	286
RseI	CAYNNNNRTG	2 cut(s)	249, 260
SaqAI	TTAA	1 cut(s)	416
SatI	GCNGC	1 cut(s)	161
Sau3AI	GATC	1 cut(s)	297
ScrFI	CCNGG	1 cut(s)	374
SetI	ASST	6 cut(s)	114, 214, 243, 465, 477, 481
SfaNI	GCATC	3 cut(s)	205, 241, 327
Sfr274I	CTCGAG	1 cut(s)	286
SlaI	CTCGAG	1 cut(s)	286
SmiMI	CAYNNNNRTG	2 cut(s)	249, 260
SmlI	CTYRAG	2 cut(s)	286, 395
SmoI	CTYRAG	2 cut(s)	286, 395
Sse9I	AATT	3 cut(s)	327, 347, 434
SsiI	CCGC	2 cut(s)	18, 160
StyD4I	CCNGG	1 cut(s)	372
TaiI	ACGT	2 cut(s)	243, 477
TaqI	TCGA	5 cut(s)	123, 131, 176, 287, 296
TasI	AATT	3 cut(s)	327, 347, 434
TauI	GCSGC	1 cut(s)	163
Tru1I	TTAA	1 cut(s)	416
Tru9I	TTAA	1 cut(s)	416
TscAI	CASTG	1 cut(s)	493
TspDTI	ATGAA	1 cut(s)	324
TspRI	CASTG	1 cut(s)	493
XapI	RAATTY	1 cut(s)	434
XhoI	CTCGAG	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.