Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003403

Physical Location & Seq

Genomic Coordinates Forward (+)

6791242 .. 6793394

Gene Structure 2153 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1097300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGCTTCGCTTCAGCTTCAACTTCCATTCTCTGCCGCTCCGTGCCTACCCAATTCCCGGTTCAGACGCAGTCCGGTCCGGTCCTCCACCGAACGCCAGGCGCTCTTTAACCGCATTGCTCCTGTCTACGATAACTTAAATGATTTGTTGAGCTTGGGTCAGCATCGAATATGGAAAAGAATGGCAGTTTCGTGGAGCGGAGCAAAAATGGGGGAGAGTGTGTTGGATTTGTGCTGTGGGAGTGGGGATTTAGCGTTTCTCTTGTCTGAGAAAGTTGGGTCCAATGGCAAGGTGATTGGTCTTGATTTCTCGAAGAAGCAGTTGTCAGTTGCTTCTTCGCGGCAGAAGTTGAAGTCGAAAGCCTGCTACTTGAACATTGAGTGGGTTGAAGGTGATGCAACTGAATTGCCGTTTTCTGACGGTCACTTTGATGCCATCACTATGGCTTATGGGCTAAGAAATGTAGTAGACAAACACAAGGCCATGGAGGAAATTTTTAGAGTTTTGAAAGCAGGCTCAAGGGTATCGATCCTTGACTTCAATAAAAGCACCAATCCAATTGTTTCATCAGTTCAGGACTTGATGATTGACAATGTTGTTGTCCCCGTGGCATCTGGTTTTGGACTTGAGGAGGATTACAAGTATTTAAAAATTTCAATCACAGAATTCCTGACAGGGGACGAGTTGGAGAAGCTGGCTTTAGATGTAGGTTTTTCCAATGCCAGATATTATGAGATTGGCGGAAGCCTCATGGGGAACTTAGTAGCCACCCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0008168 GO:0008169 GO:0008171 GO:0009058 GO:0009108 GO:0009110 GO:0009507 GO:0009536 GO:0009987 GO:0016740 GO:0016741 GO:0032259 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051188 GO:0052624 GO:0071704 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

Ubie_methyltran

Protein Analysis

259

Amino Acids

28.47

Weight (kDa)

7.64

Isoelectric Point (pI)

49.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubie_methyltran	PF01209	32 - 251	3.1e-60	ubiE/COQ5 methyltransferase family
PCMT	PF01135	62 - 151	2.2e-06	Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_23	PF13489	69 - 240	1.2e-10	Methyltransferase domain
Methyltransf_31	PF13847	70 - 186	2.2e-24	Methyltransferase domain
Methyltransf_25	PF13649	74 - 172	1.1e-21	Methyltransferase domain
Methyltransf_11	PF08241	75 - 175	2e-19	Methyltransferase domain
Methyltransf_12	PF08242	75 - 174	1.7e-12	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23360 AT1G23360 AT1G23360
fragaria_vesca	FvH4_4g26051 FvH4_4g26060 FvH4_4g26080
malus_domestica	MD13G1095900.v1.1 MD13G1096300.v1.1 MD16G1097300.v1.1
prunus_persica	Prupe.1G249500_v2.0.a1
pyrus_communis	pycom13g08360 pycom13g08390 pycom16g08290
rosa_chinensis	RchiOBHm_Chr2g0132031 RchiOBHm_Chr4g0433541 RchiOBHm_Chr6g0273261
rosa_laevigata	RLG00000002005 RLG00000006744 RLG00000033410
rosa_multiflora	Rmu_sc0000312.1_g000010 Rmu_sc0009599.1_g000015
rosa_roxburghii	Rroxscaffold_3G00246010 Rroxscaffold_4G00300010
rosa_rugosa	Rorug04G0269700
rosa_samantha	Rh2BG361000 Rh4AG164200 Rh4AG324400 Rh4BG332300 Rh4CG347600 Rh4DG328600 Rh5AG111900 Rh5AG129600 Rh6AG348700
rosa_wichuraiana	Rw0G020180 Rw4G028090 Rw6G041620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	38, 198
AccI	GTMKAC	2 cut(s)	126, 468
AccII	CGCG	1 cut(s)	340
AciI	CCGC	5 cut(s)	36, 112, 198, 340, 741
AclWI	GGATC	1 cut(s)	523
AcsI	RAATTY	3 cut(s)	492, 651, 665
AfiI	CCNNNNNNNGG	2 cut(s)	57, 676
AgsI	TTSAA	7 cut(s)	20, 352, 373, 389, 508, 541, 657
AjnI	CCWGG	1 cut(s)	96
AjuI	GAANNNNNNNTTGG	2 cut(s)	44, 76
AluBI	AGCT	3 cut(s)	16, 153, 694
AluI	AGCT	3 cut(s)	16, 153, 694
AlwI	GGATC	1 cut(s)	523
AoxI	GGCC	1 cut(s)	480
ApoI	RAATTY	3 cut(s)	492, 651, 665
ArsI	GACNNNNNNTTYG	2 cut(s)	410, 442
AspLEI	GCGC	1 cut(s)	103
AspS9I	GGNCC	3 cut(s)	76, 81, 279
AsuC2I	CCSGG	1 cut(s)	58
AsuHPI	GGTGA	2 cut(s)	304, 404
AvaII	GGWCC	3 cut(s)	76, 81, 279
BccI	CCATC	1 cut(s)	443
BceAI	ACGGC	1 cut(s)	394
BciT130I	CCWGG	1 cut(s)	98
BcnI	CCSGG	1 cut(s)	58
BfoI	RGCGCY	1 cut(s)	104
BisI	GCNGC	2 cut(s)	36, 341
BlsI	GCNGC	2 cut(s)	37, 342
Bme1390I	CCNGG	2 cut(s)	58, 98
Bme18I	GGWCC	3 cut(s)	76, 81, 279
BmgT120I	GGNCC	3 cut(s)	76, 81, 279
BmiI	GGNNCC	1 cut(s)	280
BmrFI	CCNGG	2 cut(s)	58, 98
BmsI	GCATC	4 cut(s)	172, 385, 421, 620
BpuEI	CTTGAG	2 cut(s)	502, 647
BpuMI	CCSGG	1 cut(s)	58
Bsa29I	ATCGAT	1 cut(s)	527
BsaJI	CCNNGG	2 cut(s)	483, 606
BsaWI	WCCGGW	2 cut(s)	73, 78
BsaXI	ACNNNNNCTCC	2 cut(s)	206, 236
Bsc4I	CCNNNNNNNGG	2 cut(s)	57, 676
Bse3DI	GCAATG	1 cut(s)	114
BseBI	CCWGG	1 cut(s)	98
BseCI	ATCGAT	1 cut(s)	527
BseDI	CCNNGG	2 cut(s)	483, 606
BseLI	CCNNNNNNNGG	2 cut(s)	57, 676
BseMI	GCAATG	1 cut(s)	114
BseMII	CTCAG	1 cut(s)	258
BseRI	GAGGAG	1 cut(s)	644
Bsh1236I	CGCG	1 cut(s)	340
BshFI	GGCC	1 cut(s)	482
BshVI	ATCGAT	1 cut(s)	527
BsiSI	CCGG	3 cut(s)	58, 74, 79
BslFI	GGGAC	2 cut(s)	587, 692
BslI	CCNNNNNNNGG	2 cut(s)	57, 676
BsmFI	GGGAC	2 cut(s)	587, 692
BsnI	GGCC	1 cut(s)	482
Bsp143I	GATC	1 cut(s)	528
Bsp19I	CCATGG	1 cut(s)	483
BspACI	CCGC	5 cut(s)	36, 112, 198, 340, 741
BspANI	GGCC	1 cut(s)	482
BspCNI	CTCAG	1 cut(s)	259
BspDI	ATCGAT	1 cut(s)	527
BspFNI	CGCG	1 cut(s)	340
BspLI	GGNNCC	1 cut(s)	280
BspPI	GGATC	1 cut(s)	523
BsrBI	CCGCTC	2 cut(s)	38, 198
BsrDI	GCAATG	1 cut(s)	114
BssECI	CCNNGG	2 cut(s)	483, 606
BssMI	GATC	1 cut(s)	528
BssT1I	CCWWGG	1 cut(s)	483
Bst2UI	CCWGG	1 cut(s)	98
Bst4CI	ACNGT	1 cut(s)	422
BstC8I	GCNNGC	3 cut(s)	364, 514, 696
BstDEI	CTNAG	3 cut(s)	267, 455, 760
BstDSI	CCRYGG	2 cut(s)	483, 606
BstFNI	CGCG	1 cut(s)	340
BstH2I	RGCGCY	1 cut(s)	104
BstHHI	GCGC	1 cut(s)	103
BstKTI	GATC	1 cut(s)	531
BstMBI	GATC	1 cut(s)	528
BstNI	CCWGG	1 cut(s)	98
BstSCI	CCNGG	2 cut(s)	56, 96
BstUI	CGCG	1 cut(s)	340
BstXI	CCANNNNNNTGG	1 cut(s)	442
Bsu15I	ATCGAT	1 cut(s)	527
BsuRI	GGCC	1 cut(s)	482
BsuTUI	ATCGAT	1 cut(s)	527
BtgI	CCRYGG	2 cut(s)	483, 606
Cac8I	GCNNGC	3 cut(s)	364, 514, 696
CfoI	GCGC	1 cut(s)	103
Cfr13I	GGNCC	3 cut(s)	76, 81, 279
ClaI	ATCGAT	1 cut(s)	527
CpoI	CGGWCCG	1 cut(s)	76
CseI	GACGC	1 cut(s)	75
CspI	CGGWCCG	1 cut(s)	76
CviAII	CATG	2 cut(s)	484, 751
DdeI	CTNAG	3 cut(s)	267, 455, 760
DpnI	GATC	1 cut(s)	530
DpnII	GATC	1 cut(s)	528
DraI	TTTAAA	1 cut(s)	648
EciI	GGCGGA	1 cut(s)	756
Eco130I	CCWWGG	1 cut(s)	483
Eco47I	GGWCC	3 cut(s)	76, 81, 279
EcoRI	GAATTC	1 cut(s)	665
EcoRII	CCWGG	1 cut(s)	96
EcoT14I	CCWWGG	1 cut(s)	483
ErhI	CCWWGG	1 cut(s)	483
FaeI	CATG	2 cut(s)	487, 754
FaiI	YATR	6 cut(s)	172, 443, 450, 485, 732, 752
FaqI	GGGAC	2 cut(s)	587, 692
FatI	CATG	2 cut(s)	483, 750
FblI	GTMKAC	2 cut(s)	126, 468
Fnu4HI	GCNGC	2 cut(s)	36, 341
Fsp4HI	GCNGC	2 cut(s)	36, 341
GlaI	GCGC	1 cut(s)	102
GluI	GCNGC	2 cut(s)	36, 341
HaeII	RGCGCY	1 cut(s)	104
HaeIII	GGCC	1 cut(s)	482
HapII	CCGG	3 cut(s)	58, 74, 79
HgaI	GACGC	1 cut(s)	75
HhaI	GCGC	1 cut(s)	103
Hin1II	CATG	2 cut(s)	487, 754
Hin6I	GCGC	1 cut(s)	101
HinP1I	GCGC	1 cut(s)	101
HpaII	CCGG	3 cut(s)	58, 74, 79
HphI	GGTGA	2 cut(s)	304, 404
Hpy166II	GTNNAC	2 cut(s)	127, 469
Hpy188I	TCNGA	3 cut(s)	65, 268, 418
Hpy188III	TCNNGA	4 cut(s)	302, 310, 575, 670
Hpy8I	GTNNAC	2 cut(s)	127, 469
HpyAV	CCTTC	1 cut(s)	383
HpyCH4III	ACNGT	1 cut(s)	422
HpyCH4V	TGCA	1 cut(s)	398
HpyF3I	CTNAG	3 cut(s)	267, 455, 760
Hsp92II	CATG	2 cut(s)	487, 754
HspAI	GCGC	1 cut(s)	101
Kzo9I	GATC	1 cut(s)	528
LmnI	GCTCC	4 cut(s)	43, 124, 195, 200
LweI	GCATC	4 cut(s)	172, 385, 421, 620
MaeIII	GTNAC	1 cut(s)	422
MalI	GATC	1 cut(s)	530
MbiI	CCGCTC	2 cut(s)	38, 198
MboI	GATC	1 cut(s)	528
MboII	GAAGA	2 cut(s)	325, 327
MfeI	CAATTG	1 cut(s)	558
MluCI	AATT	6 cut(s)	52, 404, 492, 558, 651, 665
MmeI	TCCRAC	2 cut(s)	204, 666
MnlI	CCTC	5 cut(s)	94, 481, 622, 625, 758
MseI	TTAA	3 cut(s)	108, 137, 647
MslI	CAYNNNNRTG	2 cut(s)	429, 440
MspI	CCGG	3 cut(s)	58, 74, 79
MspR9I	CCNGG	2 cut(s)	58, 98
MunI	CAATTG	1 cut(s)	558
MvaI	CCWGG	1 cut(s)	98
MvnI	CGCG	1 cut(s)	340
NciI	CCSGG	1 cut(s)	58
NcoI	CCATGG	1 cut(s)	483
NdeII	GATC	1 cut(s)	528
NlaIII	CATG	2 cut(s)	487, 754
NlaIV	GGNNCC	1 cut(s)	280
NmuCI	GTSAC	1 cut(s)	422
PflFI	GACNNNGTC	1 cut(s)	69
PkrI	GCNGC	2 cut(s)	37, 342
Psp6I	CCWGG	1 cut(s)	96
PspGI	CCWGG	1 cut(s)	96
PspN4I	GGNNCC	1 cut(s)	280
PspPI	GGNCC	3 cut(s)	76, 81, 279
PsyI	GACNNNGTC	1 cut(s)	69
RseI	CAYNNNNRTG	2 cut(s)	429, 440
Rsr2I	CGGWCCG	1 cut(s)	76
RsrII	CGGWCCG	1 cut(s)	76
SaqAI	TTAA	3 cut(s)	108, 137, 647
SatI	GCNGC	2 cut(s)	36, 341
Sau3AI	GATC	1 cut(s)	528
Sau96I	GGNCC	3 cut(s)	76, 81, 279
ScrFI	CCNGG	2 cut(s)	58, 98
SetI	ASST	6 cut(s)	18, 155, 294, 394, 696, 712
SfaNI	GCATC	4 cut(s)	172, 385, 421, 620
SinI	GGWCC	3 cut(s)	76, 81, 279
SmiMI	CAYNNNNRTG	2 cut(s)	429, 440
SmlI	CTYRAG	2 cut(s)	517, 626
SmoI	CTYRAG	2 cut(s)	517, 626
Sse9I	AATT	6 cut(s)	52, 404, 492, 558, 651, 665
SsiI	CCGC	5 cut(s)	36, 112, 198, 340, 741
StyD4I	CCNGG	2 cut(s)	56, 96
StyI	CCWWGG	1 cut(s)	483
TaaI	ACNGT	1 cut(s)	422
TaqI	TCGA	4 cut(s)	166, 311, 356, 527
TasI	AATT	6 cut(s)	52, 404, 492, 558, 651, 665
TauI	GCSGC	2 cut(s)	38, 343
Tru1I	TTAA	3 cut(s)	108, 137, 647
Tru9I	TTAA	3 cut(s)	108, 137, 647
TseFI	GTSAC	1 cut(s)	422
Tsp45I	GTSAC	1 cut(s)	422
TspDTI	ATGAA	1 cut(s)	555
TspGWI	ACGGA	1 cut(s)	30
Tth111I	GACNNNGTC	1 cut(s)	69
VpaK11BI	GGWCC	3 cut(s)	76, 81, 279
XapI	RAATTY	3 cut(s)	492, 651, 665
XmiI	GTMKAC	2 cut(s)	126, 468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.