MD13G1283400.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001790
Physical Location & Seq
Reverse (-)
41765336 .. 41766543
1208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1283400.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGCCTTAGAGGAGGAAGTAGATAGCTTGTTCGAAAGCGCCATGAAAGGGCAGTGGGGAAAGGTGGTGGAAGCCTACAAAAACAGTTCCAAGGCTCAAGAGGCCAAGATAACCAAATCAAACGAGACTGCTCTACACATTGCAATTGCAGATGGCCAAACAGAAATCGCAATCGAGTTGCTCAGCATCATTTCCCTCGGAGGAAATGCATCCAAAATATTCAACTTGGGGAATGAGAAAGGCAACACAGCTCTCCATCTGGCTGCCAGGCTTAATTACGTGCAACTTTGCGAAATCATGGCTACGAAGGATCAAAACCTCGTCTCCGTACGCAACCTGGACGGTGAGACCCCTCTCTTCTTGGCAGCTCATAATGGCAACATAAAGTCTTTCCTTTGTCTTCAAATTCACTGCCAAGAAAAGTTCCATTCCCTTAGAGACAACAATGGTGATACCATTCTCCATGCTGCTATCTCTGGTGAATACTTCTGTTTGGCGTTTCAGATAATTCGATTGTACCCGGAACTCGCTAACTCTATGAATGAAAACGGTTTATCTCCGCTGCATATTCTGGCCGGAAAGCCTAATGCATTCAAAAGCAGTACTCGCCTTGGAATAGCTGATGAGATTATATACCGCTGTTTGATCGTTGATAATCTCAAAATTGAAGCATATAATCATGAAGCTTGTCTGCACAAGGCAGGAGAACAAAACAGTTTGAAGTATCCGGCAAACTACGAAACTTGCATGAACTTCTTCCGGCATCTGAGAAGTCTGTTCAAAGTGTTAAGTGGCGAAATCACGGGGAAAAAAGAATTGTACCCAAGCTTTGCGCGTATACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001508 GO:0002027 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009893 GO:0009987 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042383 GO:0042391 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 PGG

Protein Analysis

283

Amino Acids

31.36

Weight (kDa)

6.22

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 33 - 105 4.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 110 - 162 4.2e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 838
AccII CGCG 1 cut(s) 835
AciI CCGC 2 cut(s) 560, 637
AclWI GGATC 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 154, 573
AcsI RAATTY 1 cut(s) 405
AfaI GTAC 4 cut(s) 330, 518, 604, 821
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 6 cut(s) 223, 404, 595, 668, 721, 781
AjnI CCWGG 2 cut(s) 266, 336
AluBI AGCT 6 cut(s) 27, 251, 368, 620, 686, 828
AluI AGCT 6 cut(s) 27, 251, 368, 620, 686, 828
Alw26I GTCTC 4 cut(s) 119, 328, 341, 432
AlwI GGATC 1 cut(s) 318
AoxI GGCC 4 cut(s) 3, 102, 154, 573
ApeKI GCWGC 4 cut(s) 263, 365, 467, 562
ApoI RAATTY 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 755
AspLEI GCGC 2 cut(s) 41, 835
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 3 cut(s) 356, 461, 491
AsuII TTCGAA 1 cut(s) 33
BalI TGGCCA 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 392
BbvI GCAGC 4 cut(s) 250, 377, 454, 549
BccI CCATC 2 cut(s) 146, 264
BciT130I CCWGG 2 cut(s) 268, 338
BciVI GTATCC 1 cut(s) 735
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 4 cut(s) 119, 328, 341, 432
BfoI RGCGCY 1 cut(s) 42
BfuI GTATCC 1 cut(s) 735
BisI GCNGC 4 cut(s) 264, 366, 468, 563
BlpI GCTNAGC 1 cut(s) 182
BlsI GCNGC 4 cut(s) 265, 367, 469, 564
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 3 cut(s) 268, 338, 521
BmrFI CCNGG 3 cut(s) 268, 338, 521
BmsI GCATC 3 cut(s) 195, 218, 772
BpiI GAAGAC 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 521
BsaAI YACGTR 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 3 cut(s) 90, 196, 610
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 1 cut(s) 846
Bse3DI GCAATG 1 cut(s) 138
BseBI CCWGG 2 cut(s) 268, 338
BseDI CCNNGG 3 cut(s) 90, 196, 610
BseGI GGATG 1 cut(s) 209
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 2 cut(s) 196, 758
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 4 cut(s) 250, 377, 454, 549
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 1 cut(s) 835
BshFI GGCC 4 cut(s) 5, 104, 156, 575
BsiSI CCGG 4 cut(s) 521, 576, 728, 760
BsiWI CGTACG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 4 cut(s) 119, 328, 341, 432
BsmBI CGTCTC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 4 cut(s) 5, 104, 156, 575
Bso31I GGTCTC 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 33
Bsp143I GATC 2 cut(s) 310, 645
Bsp1720I GCTNAGC 1 cut(s) 182
BspACI CCGC 2 cut(s) 560, 637
BspANI GGCC 4 cut(s) 5, 104, 156, 575
BspCNI CTCAG 2 cut(s) 195, 759
BspFNI CGCG 1 cut(s) 835
BspHI TCATGA 1 cut(s) 679
BspPI GGATC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 33
BspTNI GGTCTC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 138
BsrI ACTGG 1 cut(s) 846
BssECI CCNNGG 3 cut(s) 90, 196, 610
BssMI GATC 2 cut(s) 310, 645
BssNAI GTATAC 1 cut(s) 839
BssT1I CCWWGG 2 cut(s) 90, 610
Bst1107I GTATAC 1 cut(s) 839
Bst2UI CCWGG 2 cut(s) 268, 338
Bst4CI ACNGT 4 cut(s) 86, 344, 551, 716
Bst6I CTCTTC 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 33
BstDEI CTNAG 4 cut(s) 7, 182, 434, 767
BstF5I GGATG 1 cut(s) 209
BstFNI CGCG 1 cut(s) 835
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 2 cut(s) 41, 835
BstKTI GATC 2 cut(s) 313, 648
BstMAI GTCTC 4 cut(s) 119, 328, 341, 432
BstMBI GATC 2 cut(s) 310, 645
BstMWI GCNNNNNNNGC 2 cut(s) 101, 606
BstNI CCWGG 2 cut(s) 268, 338
BstSCI CCNGG 3 cut(s) 266, 336, 519
BstUI CGCG 1 cut(s) 835
BstV1I GCAGC 4 cut(s) 250, 377, 454, 549
BstV2I GAAGAC 1 cut(s) 392
BstZ17I GTATAC 1 cut(s) 839
BsuI GTATCC 1 cut(s) 735
BsuRI GGCC 4 cut(s) 5, 104, 156, 575
BtsCI GGATG 1 cut(s) 209
BtsI GCAGTG 2 cut(s) 59, 409
BtsIMutI CAGTG 2 cut(s) 59, 409
CciI TCATGA 1 cut(s) 679
CfoI GCGC 2 cut(s) 41, 835
Csp6I GTAC 4 cut(s) 329, 517, 603, 820
CviAII CATG 5 cut(s) 43, 298, 464, 680, 748
CviQI GTAC 4 cut(s) 329, 517, 603, 820
DdeI CTNAG 4 cut(s) 7, 182, 434, 767
DpnI GATC 2 cut(s) 312, 647
DpnII GATC 2 cut(s) 310, 645
EaeI YGGCCR 2 cut(s) 154, 573
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 90, 610
Eco31I GGTCTC 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 266, 336
EcoT14I CCWWGG 2 cut(s) 90, 610
EcoT22I ATGCAT 2 cut(s) 211, 592
ErhI CCWWGG 2 cut(s) 90, 610
Esp3I CGTCTC 1 cut(s) 328
FaeI CATG 5 cut(s) 46, 301, 467, 683, 751
FatI CATG 5 cut(s) 42, 297, 463, 679, 747
FblI GTMKAC 1 cut(s) 838
Fnu4HI GCNGC 4 cut(s) 264, 366, 468, 563
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 4 cut(s) 264, 366, 468, 563
GlaI GCGC 2 cut(s) 40, 834
GluI GCNGC 4 cut(s) 264, 366, 468, 563
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 4 cut(s) 5, 104, 156, 575
HapII CCGG 4 cut(s) 521, 576, 728, 760
HhaI GCGC 2 cut(s) 41, 835
Hin1II CATG 5 cut(s) 46, 301, 467, 683, 751
Hin6I GCGC 2 cut(s) 39, 833
HinP1I GCGC 2 cut(s) 39, 833
HindIII AAGCTT 2 cut(s) 684, 826
HpaII CCGG 4 cut(s) 521, 576, 728, 760
HphI GGTGA 3 cut(s) 356, 461, 491
Hpy166II GTNNAC 1 cut(s) 839
Hpy188I TCNGA 3 cut(s) 200, 504, 768
Hpy188III TCNNGA 2 cut(s) 98, 680
Hpy8I GTNNAC 1 cut(s) 839
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 4 cut(s) 86, 344, 551, 716
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 8 cut(s) 143, 149, 209, 283, 565, 590, 694, 747
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 606
HpyF3I CTNAG 4 cut(s) 7, 182, 434, 767
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 5 cut(s) 46, 301, 467, 683, 751
HspAI GCGC 2 cut(s) 39, 833
Kzo9I GATC 2 cut(s) 310, 645
Lsp1109I GCAGC 4 cut(s) 250, 377, 454, 549
LweI GCATC 3 cut(s) 195, 218, 772
MaeII ACGT 1 cut(s) 279
MalI GATC 2 cut(s) 312, 647
MboI GATC 2 cut(s) 310, 645
MboII GAAGA 3 cut(s) 349, 392, 748
MfeI CAATTG 1 cut(s) 144
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 6 cut(s) 144, 274, 405, 507, 663, 815
MluNI TGGCCA 1 cut(s) 156
MnlI CCTC 7 cut(s) 4, 7, 94, 194, 206, 329, 363
Mox20I TGGCCA 1 cut(s) 156
Mph1103I ATGCAT 2 cut(s) 211, 592
MroXI GAANNNNTTC 1 cut(s) 755
MscI TGGCCA 1 cut(s) 156
MseI TTAA 2 cut(s) 273, 788
Msp20I TGGCCA 1 cut(s) 156
MspA1I CMGCKG 2 cut(s) 562, 639
MspI CCGG 4 cut(s) 521, 576, 728, 760
MspR9I CCNGG 3 cut(s) 268, 338, 521
MunI CAATTG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 590
MvaI CCWGG 2 cut(s) 268, 338
MvnI CGCG 1 cut(s) 835
MwoI GCNNNNNNNGC 2 cut(s) 101, 606
NciI CCSGG 1 cut(s) 521
NdeII GATC 2 cut(s) 310, 645
NlaIII CATG 5 cut(s) 46, 301, 467, 683, 751
NsiI ATGCAT 2 cut(s) 211, 592
NspV TTCGAA 1 cut(s) 33
PagI TCATGA 1 cut(s) 679
PctI GAATGC 1 cut(s) 590
PdmI GAANNNNTTC 1 cut(s) 755
Pfl23II CGTACG 1 cut(s) 328
PkrI GCNGC 4 cut(s) 265, 367, 469, 564
Ppu21I YACGTR 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 266, 336
PspGI CCWGG 2 cut(s) 266, 336
PspLI CGTACG 1 cut(s) 328
RsaI GTAC 4 cut(s) 330, 518, 604, 821
RsaNI GTAC 4 cut(s) 329, 517, 603, 820
SaqAI TTAA 2 cut(s) 273, 788
SatI GCNGC 4 cut(s) 264, 366, 468, 563
Sau3AI GATC 2 cut(s) 310, 645
ScaI AGTACT 1 cut(s) 604
ScrFI CCNGG 3 cut(s) 268, 338, 521
SfaNI GCATC 3 cut(s) 195, 218, 772
SfuI TTCGAA 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
Sse9I AATT 6 cut(s) 144, 274, 405, 507, 663, 815
SsiI CCGC 2 cut(s) 560, 637
SspI AATATT 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 266, 336, 519
StyI CCWWGG 2 cut(s) 90, 610
TaaI ACNGT 4 cut(s) 86, 344, 551, 716
TaiI ACGT 1 cut(s) 282
TaqI TCGA 3 cut(s) 33, 174, 511
TasI AATT 6 cut(s) 144, 274, 405, 507, 663, 815
TatI WGTACW 1 cut(s) 602
Tru1I TTAA 2 cut(s) 273, 788
Tru9I TTAA 2 cut(s) 273, 788
TscAI CASTG 2 cut(s) 59, 416
TseI GCWGC 4 cut(s) 263, 365, 467, 562
TspDTI ATGAA 5 cut(s) 59, 554, 558, 696, 764
TspGWI ACGGA 1 cut(s) 316
TspRI CASTG 2 cut(s) 59, 416
XapI RAATTY 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 838
XmnI GAANNNNTTC 1 cut(s) 755
ZrmI AGTACT 1 cut(s) 604
Zsp2I ATGCAT 2 cut(s) 211, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.