Rorug04G0126200

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001790
Physical Location & Seq
Forward (+)
20051656 .. 20083737
32082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0126200.1

Sequence Viewer

Length: 2697 bp
ATGGTACCTACATATGAGAATGCAAAGACTTCAACAATTCCAGGTAGCTTGACCATGGCAAACCAGAAGCACTTCCTCTGGTTCATCTTCATTGTTTTGTTCATCCCTGCGATCGAGCCTTCAACAACGAAAGAGGTGATACGAGTACCAGTAGGAGTTGTTCTTAACTTAAATTCCTCAGTGGGAGCTATGGCAGAGAACTGCATGGCCATGGCACTTGATGATTTTTATGCTAAGCATGCTCATTATCAAACAAGGCTTTCCCTTCTCACCAGCGATTCAAAGAATGATGTTGTTTCGGCAGCATTTGAAGCACAGCACTTGATAAACATCGAAAGAGTGAGTGCCATTATCGGACCTCAAAGTTCAGCAGAAGCAAAGTTTGTGATAGAGCTGGGAAGAAGGAATCATGTTCCCATTATTTCGTTTTCTGCCACTAGTCCATCTCTTTCTCCATCCCATAGCTCATTTTTCGTTCGGACGGCTTTTAGTGACTCTGCTCAAGTAGAAGCCATAGCTGCCATTGTCGGAGCTTATAGTTGGTTGGAAGTTGTTCTCATCTATGAAGACACGGAGTATGGAAATAGCTTAATTCCATATTTGGTAGACGCTTTCCAGAAAGTTGGAGCTCGAGTACCTCATAGAAGTGTCATTCCTCCTCATTATAATGACAGAGAAATTTTAAAGGAGCTTAACCACTTGAAATCAACTCGAGCAAGAATATTTTTGGTCCATATGACTGCTTTCCTCGGGTCCAAATTGTTTTTATTAGCAAATAAGGCAGGGATGATGACCGAAGGGTATGGATGGATTGTTACGGAAGGGTTATCGACTTTATTAGATCCTGTGGGTTCAAGAACCATGGACTCAATGCAAGGTGTAGTGGGAGTAAGGCCACATATACCAATGTCAAAAGAACTTGAAGACTTCTTGTTGCGAATGAAAAGATTCAGGTTCAGAAAATCAAGCAAGATTACTGGTGTAAACCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGGAAGCAGAGTTGATCTTGCAAGTTTGGGAACTGGTGACAGGGGTCAGAATCTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTCGGAATTTCCGGTTGGTTAAGGGACAGTTGGAAGCTTCAACCTTTGAGGTATTTAATGTGATTGGCAGAAAAGAAAGAATAATTGGGTATTGGAACCATTCCCGAAAGCTGAATGGAGCAGCAAAGTTGGATGTAAGGAAGACATTCGAGAAACCAATCTGGCCAGGGTACACTACAGACATCCCTCCAACAAAGAACTTGAAGATTGGAGTTCCCTTGAAACAGGGTTTTAATGAATTCCTAAAATTCGAAAACAACCACCAGATATCAGGATTCTCTGCTGATATATTCTTTGCTGCAGTAAAGCAACTGCCATTTTCCCTTTCATATGAGTTTTTTTCCTTCCCGGGGACTTATGATGATCTTGTTTATCAAATTTATCGCCAGAAATATGACGCTGTGGTGGGAGACACAACAATTGTAGCTAATCGCTCAATATATGTAGATTTTACGTTGCCTTACTCTGAATCAGGAGTTTCCATGATAGTTTTGACAGAAGACAGTGAGAGGAATAGTCTTTGGATTTTCTTGAAGCCACTAAGCTTGGGTCTTTGGTTGACAACCGGCGTAGCCTTCATACTTACAGGTTTTGTAATATGGCTTCTTGAGCACCGGGAAAACACCGAGTTCAGAGGTCCTCCACAGCAACAACTTGGCATGATATTCTGGTTCTCCTTTTCAACCCTCGTCTTTGCACACAGAGAGAAAGTGGTGAACAATTGGTCAAGGTTTGTGCTGATTATATGGGTTTTTGTGGTACTGATCCTAACACAGAGTTACACTGCAAGCTTAGCCTCGATGTTAACAGTACAGAAGTTGCAGCCTGCATTCACTGATATAAAAGAGATACAAAGAAATGGTTACAAAGTAGGATATCAAAATAGTTCCTTTATTAAAGGGTTTCTCATGGAGCATTTAAATTTTGATGAGACCCAGTTGGTGCCATTGGTAACCATTCAGGATTATCATAACGCACTCTCTAGAGGAAATAAGAATAGAGTTGCTGCCATTTTAGATGAAGTCCCTTACCTCAAGTTGATCCTTAAAAAAAACTGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGATTTGGCTTTGCTTTCCAATCAGGGTCCTCGCTGGTATCCTACATGTCAAGCGCAATCTTGAACGTGACTCAAGATAAAAATCAGATGGATAAACTTCAGTCGACTTACTTTGATTACTTTGATAACCAACCTGCCTGTGAGGATCAAGGTGCCAAAATTTCTTCAGAGGGTCCAAGTCTTGGTGTCTACAGCTTTGGAGGCCTCTTCATCATCGCAGGTGTGGCCTCCATGGTTGCTCTTTTGATGTACATGTATCACTTCCTTTGCTCTCATTGGCCAGCTTTGCGAACAATTAGTTCTTCTGAGAGCAGATTTTGGGAGAAATTGGTTGAAGTGGCAAAGCATTTTGACAAGAAAGATCTCACTTCACATCAGTTTAAGCGAAGAGAATCAAGAGTACATGCTTTAGACACTCCTGATGAAGGTCTAGCAGCTGCACCGGGTGCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001508 GO:0002027 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009893 GO:0009987 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042383 GO:0042391 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 PGG

Protein Analysis

898

Amino Acids

101.05

Weight (kDa)

8.58

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 59 - 361 6.1e-21 Periplasmic binding protein
ANF_receptor PF01094 66 - 404 1.1e-75 Receptor family ligand binding region
SBP_bac_3 PF00497 465 - 786 2.9e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd PF10613 465 - 545 4.4e-06 Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan PF00060 564 - 823 2.4e-36 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AarI CACCTGC 1 cut(s) 2450
Acc36I ACCTGC 2 cut(s) 2383, 2450
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 3 cut(s) 4, 2086, 2393
AccB7I CCANNNNNTGG 1 cut(s) 2423
AccI GTMKAC 3 cut(s) 606, 2345, 2430
AclWI GGATC 4 cut(s) 836, 1903, 2179, 2394
AcoI YGGCCR 3 cut(s) 207, 1307, 2519
AcsI RAATTY 9 cut(s) 172, 678, 1130, 1150, 1382, 1391, 1521, 2065, 2400
AcuI CTGAAG 2 cut(s) 2324, 2391
AdeI CACNNNGTG 1 cut(s) 2687
AfaI GTAC 9 cut(s) 6, 147, 636, 1316, 1905, 1956, 2210, 2492, 2643
AfiI CCNNNNNNNGG 3 cut(s) 1494, 2423, 2666
AflIII ACRYGT 2 cut(s) 2286, 2493
AhlI ACTAGT 1 cut(s) 437
AjnI CCWGG 2 cut(s) 40, 1309
AjuI GAANNNNNNNTTGG 4 cut(s) 1142, 1174, 1212, 1244
Alw21I GWGCWC 2 cut(s) 631, 1758
Alw26I GTCTC 2 cut(s) 1548, 2069
AlwI GGATC 4 cut(s) 836, 1903, 2179, 2394
Ama87I CYCGRG 4 cut(s) 630, 711, 749, 1492
AoxI GGCC 6 cut(s) 207, 893, 1307, 2443, 2466, 2519
ApeKI GCWGC 8 cut(s) 302, 518, 1265, 1442, 1966, 2150, 2675, 2678
ApoI RAATTY 9 cut(s) 172, 678, 1130, 1150, 1382, 1391, 1521, 2065, 2400
Asp700I GAANNNNTTC 4 cut(s) 71, 552, 947, 1289
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 2297
AspS9I GGNCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
AsuC2I CCSGG 4 cut(s) 1493, 1494, 1760, 2685
AsuHPI GGTGA 4 cut(s) 148, 262, 1100, 1870
AsuII TTCGAA 1 cut(s) 1395
AvaI CYCGRG 4 cut(s) 630, 711, 749, 1492
AvaII GGWCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
BalI TGGCCA 3 cut(s) 209, 1309, 2521
BanI GGYRCC 3 cut(s) 4, 2086, 2393
BanII GRGCYC 1 cut(s) 631
BarI GAAGNNNNNNTAC 2 cut(s) 1731, 1763
BbsI GAAGAC 4 cut(s) 573, 930, 1292, 1650
Bbv12I GWGCWC 2 cut(s) 631, 1758
BbvI GCAGC 8 cut(s) 314, 505, 1277, 1429, 1978, 2137, 2665, 2687
BccI CCATC 5 cut(s) 451, 463, 801, 2234, 2323
BceAI ACGGC 1 cut(s) 498
BciT130I CCWGG 2 cut(s) 42, 1311
BciVI GTATCC 1 cut(s) 2290
BcnI CCSGG 4 cut(s) 1493, 1494, 1760, 2685
BcoDI GTCTC 2 cut(s) 1548, 2069
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 4 cut(s) 438, 1121, 2127, 2672
BfmI CTRYAG 3 cut(s) 1320, 1443, 2431
BfuAI ACCTGC 2 cut(s) 2383, 2450
BfuI GTATCC 1 cut(s) 2290
BglII AGATCT 1 cut(s) 2602
BisI GCNGC 8 cut(s) 303, 519, 1266, 1443, 1967, 2151, 2676, 2679
BlpI GCTNAGC 2 cut(s) 234, 1936
BlsI GCNGC 8 cut(s) 304, 520, 1267, 1444, 1968, 2152, 2677, 2680
Bme1390I CCNGG 6 cut(s) 42, 1311, 1493, 1494, 1760, 2685
Bme18I GGWCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
BmeT110I CYCGRG 4 cut(s) 630, 711, 749, 1492
BmgT120I GGNCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
BmiI GGNNCC 7 cut(s) 6, 754, 1241, 2088, 2269, 2395, 2415
BmrFI CCNGG 6 cut(s) 42, 1311, 1493, 1494, 1760, 2685
BmrI ACTGGG 1 cut(s) 2074
BmuI ACTGGG 1 cut(s) 2074
BoxI GACNNNNGTC 1 cut(s) 1095
BpiI GAAGAC 4 cut(s) 573, 930, 1292, 1650
Bpu1102I GCTNAGC 2 cut(s) 234, 1936
Bpu14I TTCGAA 1 cut(s) 1395
BpuEI CTTGAG 4 cut(s) 486, 1772, 2162, 2298
BpuMI CCSGG 4 cut(s) 1493, 1494, 1760, 2685
BsaBI GATNNNNATC 2 cut(s) 329, 2322
BsaI GGTCTC 1 cut(s) 2069
BsaJI CCNNGG 9 cut(s) 54, 210, 748, 861, 1310, 1492, 1493, 2214, 2472
BsaWI WCCGGW 1 cut(s) 1155
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 3 cut(s) 1494, 2423, 2666
Bse118I RCCGGY 1 cut(s) 1709
Bse1I ACTGG 4 cut(s) 149, 982, 1090, 2080
Bse3DI GCAATG 1 cut(s) 1035
Bse8I GATNNNNATC 2 cut(s) 329, 2322
BseBI CCWGG 2 cut(s) 42, 1311
BseDI CCNNGG 9 cut(s) 54, 210, 748, 861, 1310, 1492, 1493, 2214, 2472
BseGI GGATG 6 cut(s) 102, 455, 792, 812, 1282, 1326
BseJI GATNNNNATC 2 cut(s) 329, 2322
BseLI CCNNNNNNNGG 3 cut(s) 1494, 2423, 2666
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 3 cut(s) 192, 1156, 2538
BseNI ACTGG 4 cut(s) 149, 982, 1090, 2080
BseRI GAGGAG 1 cut(s) 648
BseXI GCAGC 8 cut(s) 314, 505, 1277, 1429, 1978, 2137, 2665, 2687
BseYI CCCAGC 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 2664
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 6 cut(s) 209, 895, 1309, 2445, 2468, 2521
BshNI GGYRCC 3 cut(s) 4, 2086, 2393
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 2 cut(s) 631, 1758
BsiHKCI CYCGRG 4 cut(s) 630, 711, 749, 1492
BsiSI CCGG 5 cut(s) 1156, 1493, 1710, 1759, 2684
BslFI GGGAC 3 cut(s) 1182, 1510, 2153
BslI CCNNNNNNNGG 3 cut(s) 1494, 2423, 2666
BsmAI GTCTC 2 cut(s) 1548, 2069
BsmFI GGGAC 3 cut(s) 1182, 1510, 2153
BsmI GAATGC 2 cut(s) 25, 1973
BsnI GGCC 6 cut(s) 209, 895, 1309, 2445, 2468, 2521
Bso31I GGTCTC 1 cut(s) 2069
BsoBI CYCGRG 4 cut(s) 630, 711, 749, 1492
Bsp119I TTCGAA 1 cut(s) 1395
Bsp1286I GDGCHC 2 cut(s) 631, 1758
Bsp1407I TGTACA 1 cut(s) 2490
Bsp143I GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
Bsp1720I GCTNAGC 2 cut(s) 234, 1936
Bsp19I CCATGG 5 cut(s) 54, 210, 861, 2214, 2472
BspANI GGCC 6 cut(s) 209, 895, 1309, 2445, 2468, 2521
BspCNI CTCAG 3 cut(s) 191, 1155, 2539
BspLI GGNNCC 7 cut(s) 6, 754, 1241, 2088, 2269, 2395, 2415
BspMAI CTGCAG 1 cut(s) 1447
BspMI ACCTGC 2 cut(s) 2383, 2450
BspPI GGATC 4 cut(s) 836, 1903, 2179, 2394
BspT104I TTCGAA 1 cut(s) 1395
BspT107I GGYRCC 3 cut(s) 4, 2086, 2393
BspTNI GGTCTC 1 cut(s) 2069
BsrDI GCAATG 1 cut(s) 1035
BsrFI RCCGGY 1 cut(s) 1709
BsrGI TGTACA 1 cut(s) 2490
BsrI ACTGG 4 cut(s) 149, 982, 1090, 2080
BssAI RCCGGY 1 cut(s) 1709
BssECI CCNNGG 9 cut(s) 54, 210, 748, 861, 1310, 1492, 1493, 2214, 2472
BssMI GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
BssT1I CCWWGG 5 cut(s) 54, 210, 861, 2214, 2472
Bst2UI CCWGG 2 cut(s) 42, 1311
Bst4CI ACNGT 5 cut(s) 1015, 1173, 1649, 1954, 2201
Bst6I CTCTTC 2 cut(s) 2453, 2623
BstAPI GCANNNNNTGC 1 cut(s) 2687
BstAUI TGTACA 1 cut(s) 2490
BstBI TTCGAA 1 cut(s) 1395
BstC8I GCNNGC 4 cut(s) 240, 1933, 1971, 2523
BstDEI CTNAG 6 cut(s) 178, 234, 1142, 1685, 1936, 2547
BstDSI CCRYGG 5 cut(s) 54, 210, 861, 2214, 2472
BstEII GGTNACC 1 cut(s) 2095
BstENI CCTNNNNNAGG 1 cut(s) 2664
BstF5I GGATG 6 cut(s) 102, 455, 792, 812, 1282, 1326
BstHHI GCGC 1 cut(s) 2297
BstKTI GATC 8 cut(s) 114, 844, 1069, 1510, 1911, 2187, 2389, 2605
BstMAI GTCTC 2 cut(s) 1548, 2069
BstMBI GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
BstMCI CGRYCG 1 cut(s) 114
BstNI CCWGG 2 cut(s) 42, 1311
BstNSI RCATGY 4 cut(s) 242, 2290, 2497, 2648
BstPAI GACNNNNGTC 1 cut(s) 1095
BstPI GGTNACC 1 cut(s) 2095
BstSCI CCNGG 6 cut(s) 40, 1309, 1491, 1492, 1758, 2683
BstSFI CTRYAG 3 cut(s) 1320, 1443, 2431
BstV1I GCAGC 8 cut(s) 314, 505, 1277, 1429, 1978, 2137, 2665, 2687
BstV2I GAAGAC 4 cut(s) 573, 930, 1292, 1650
BstX2I RGATCY 2 cut(s) 841, 2602
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 2 cut(s) 841, 2602
BsuI GTATCC 1 cut(s) 2290
BsuRI GGCC 6 cut(s) 209, 895, 1309, 2445, 2468, 2521
BtgI CCRYGG 5 cut(s) 54, 210, 861, 2214, 2472
BtgZI GCGATG 1 cut(s) 2440
BtsCI GGATG 6 cut(s) 102, 455, 792, 812, 1282, 1326
BtsI GCAGTG 2 cut(s) 1041, 1926
BtsIMutI CAGTG 5 cut(s) 186, 1041, 1654, 1926, 1977
BveI ACCTGC 2 cut(s) 2383, 2450
Cac8I GCNNGC 4 cut(s) 240, 1933, 1971, 2523
CfoI GCGC 1 cut(s) 2297
Cfr10I RCCGGY 1 cut(s) 1709
Cfr13I GGNCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
Cfr9I CCCGGG 1 cut(s) 1492
CseI GACGC 2 cut(s) 617, 1550
Csp6I GTAC 9 cut(s) 5, 146, 635, 1315, 1904, 1955, 2209, 2491, 2642
CspCI CAANNNNNGTGG 4 cut(s) 1017, 1052, 1671, 1706
CviQI GTAC 9 cut(s) 5, 146, 635, 1315, 1904, 1955, 2209, 2491, 2642
DdeI CTNAG 6 cut(s) 178, 234, 1142, 1685, 1936, 2547
DpnI GATC 8 cut(s) 113, 843, 1068, 1509, 1910, 2186, 2388, 2604
DpnII GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
DraI TTTAAA 3 cut(s) 684, 1128, 2064
DraIII CACNNNGTG 1 cut(s) 2687
EaeI YGGCCR 3 cut(s) 207, 1307, 2519
Eam1104I CTCTTC 2 cut(s) 2453, 2623
EarI CTCTTC 2 cut(s) 2453, 2623
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 5 cut(s) 54, 210, 861, 2214, 2472
Eco147I AGGCCT 1 cut(s) 2445
Eco24I GRGCYC 1 cut(s) 631
Eco31I GGTCTC 1 cut(s) 2069
Eco32I GATATC 2 cut(s) 1413, 2021
Eco47I GGWCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 2324, 2391
Eco88I CYCGRG 4 cut(s) 630, 711, 749, 1492
Eco91I GGTNACC 1 cut(s) 2095
EcoICRI GAGCTC 1 cut(s) 629
EcoNI CCTNNNNNAGG 1 cut(s) 2664
EcoO109I RGGNCCY 2 cut(s) 1781, 2268
EcoO65I GGTNACC 1 cut(s) 2095
EcoRI GAATTC 1 cut(s) 1382
EcoRII CCWGG 2 cut(s) 40, 1309
EcoRV GATATC 2 cut(s) 1413, 2021
EcoT14I CCWWGG 5 cut(s) 54, 210, 861, 2214, 2472
EcoT38I GRGCYC 1 cut(s) 631
ErhI CCWWGG 5 cut(s) 54, 210, 861, 2214, 2472
FaqI GGGAC 3 cut(s) 1182, 1510, 2153
FauNDI CATATG 4 cut(s) 13, 735, 1006, 1474
FblI GTMKAC 3 cut(s) 606, 2345, 2430
Fnu4HI GCNGC 8 cut(s) 303, 519, 1266, 1443, 1967, 2151, 2676, 2679
FokI GGATG 6 cut(s) 89, 442, 799, 819, 1289, 1313
FriOI GRGCYC 1 cut(s) 631
Fsp4HI GCNGC 8 cut(s) 303, 519, 1266, 1443, 1967, 2151, 2676, 2679
FspBI CTAG 4 cut(s) 438, 1121, 2127, 2672
GlaI GCGC 1 cut(s) 2296
GluI GCNGC 8 cut(s) 303, 519, 1266, 1443, 1967, 2151, 2676, 2679
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 6 cut(s) 209, 895, 1309, 2445, 2468, 2521
HapII CCGG 5 cut(s) 1156, 1493, 1710, 1759, 2684
HgaI GACGC 2 cut(s) 617, 1550
HhaI GCGC 1 cut(s) 2297
Hin6I GCGC 1 cut(s) 2295
HinP1I GCGC 1 cut(s) 2295
HincII GTYRAC 3 cut(s) 1704, 1950, 2346
HindII GTYRAC 3 cut(s) 1704, 1950, 2346
HindIII AAGCTT 3 cut(s) 1179, 1687, 1933
HpaI GTTAAC 1 cut(s) 1950
HpaII CCGG 5 cut(s) 1156, 1493, 1710, 1759, 2684
HphI GGTGA 4 cut(s) 148, 262, 1100, 1870
Hpy166II GTNNAC 9 cut(s) 607, 985, 1317, 1704, 1861, 1950, 2211, 2346, 2431
Hpy8I GTNNAC 9 cut(s) 607, 985, 1317, 1704, 1861, 1950, 2211, 2346, 2431
HpyAV CCTTC 9 cut(s) 129, 275, 396, 791, 815, 1036, 1498, 1729, 2660
HpyCH4III ACNGT 5 cut(s) 1015, 1173, 1649, 1954, 2201
HpyCH4IV ACGT 2 cut(s) 1598, 2307
HpyF3I CTNAG 6 cut(s) 178, 234, 1142, 1685, 1936, 2547
HpySE526I ACGT 2 cut(s) 1598, 2307
HspAI GCGC 1 cut(s) 2295
KpnI GGTACC 1 cut(s) 8
KspAI GTTAAC 1 cut(s) 1950
Kzo9I GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
LmnI GCTCC 6 cut(s) 185, 530, 626, 688, 1262, 2056
Lsp1109I GCAGC 8 cut(s) 314, 505, 1277, 1429, 1978, 2137, 2665, 2687
MaeI CTAG 4 cut(s) 438, 1121, 2127, 2672
MaeII ACGT 2 cut(s) 1598, 2307
MaeIII GTNAC 7 cut(s) 491, 814, 1088, 1922, 2006, 2095, 2308
MalI GATC 8 cut(s) 113, 843, 1068, 1509, 1910, 2186, 2388, 2604
MboI GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
MfeI CAATTG 2 cut(s) 1563, 1864
MflI RGATCY 2 cut(s) 841, 2602
MhlI GDGCHC 2 cut(s) 631, 1758
MlsI TGGCCA 3 cut(s) 209, 1309, 2521
MluNI TGGCCA 3 cut(s) 209, 1309, 2521
MlyI GAGTC 3 cut(s) 488, 860, 2305
MmeI TCCRAC 9 cut(s) 508, 525, 604, 1027, 1127, 1155, 1254, 1358, 2200
Mox20I TGGCCA 3 cut(s) 209, 1309, 2521
MroXI GAANNNNTTC 4 cut(s) 71, 552, 947, 1289
MscI TGGCCA 3 cut(s) 209, 1309, 2521
MslI CAYNNNNRTG 3 cut(s) 209, 645, 666
Msp20I TGGCCA 3 cut(s) 209, 1309, 2521
MspA1I CMGCKG 1 cut(s) 2678
MspI CCGG 5 cut(s) 1156, 1493, 1710, 1759, 2684
MspR9I CCNGG 6 cut(s) 42, 1311, 1493, 1494, 1760, 2685
MunI CAATTG 2 cut(s) 1563, 1864
Mva1269I GAATGC 2 cut(s) 25, 1973
MvaI CCWGG 2 cut(s) 42, 1311
NciI CCSGG 4 cut(s) 1493, 1494, 1760, 2685
NcoI CCATGG 5 cut(s) 54, 210, 861, 2214, 2472
NdeI CATATG 4 cut(s) 13, 735, 1006, 1474
NdeII GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
NlaIV GGNNCC 7 cut(s) 6, 754, 1241, 2088, 2269, 2395, 2415
NmuCI GTSAC 3 cut(s) 491, 1088, 2308
NspI RCATGY 4 cut(s) 242, 2290, 2497, 2648
NspV TTCGAA 1 cut(s) 1395
PaeI GCATGC 1 cut(s) 242
PaeR7I CTCGAG 2 cut(s) 630, 711
PaqCI CACCTGC 1 cut(s) 2450
PceI AGGCCT 1 cut(s) 2445
PciI ACATGT 2 cut(s) 2286, 2493
PctI GAATGC 2 cut(s) 25, 1973
PdmI GAANNNNTTC 4 cut(s) 71, 552, 947, 1289
PfeI GAWTC 7 cut(s) 278, 406, 948, 1102, 1419, 1613, 2633
PflMI CCANNNNNTGG 1 cut(s) 2423
PkrI GCNGC 8 cut(s) 304, 520, 1267, 1444, 1968, 2152, 2677, 2680
Ple19I CGATCG 1 cut(s) 114
PleI GAGTC 3 cut(s) 488, 860, 2305
PpsI GAGTC 3 cut(s) 488, 860, 2305
PpuMI RGGWCCY 2 cut(s) 1781, 2268
PscI ACATGT 2 cut(s) 2286, 2493
PshAI GACNNNNGTC 1 cut(s) 1095
PsiI TTATAA 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 631
Psp5II RGGWCCY 2 cut(s) 1781, 2268
Psp6I CCWGG 2 cut(s) 40, 1309
PspEI GGTNACC 1 cut(s) 2095
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 40, 1309
PspN4I GGNNCC 7 cut(s) 6, 754, 1241, 2088, 2269, 2395, 2415
PspPI GGNCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
PspPPI RGGWCCY 2 cut(s) 1781, 2268
PspXI VCTCGAGB 2 cut(s) 630, 711
PsrI GAACNNNNNNTAC 2 cut(s) 144, 176
PstI CTGCAG 1 cut(s) 1447
PsuI RGATCY 2 cut(s) 841, 2602
PvuI CGATCG 1 cut(s) 114
PvuII CAGCTG 1 cut(s) 2678
RsaI GTAC 9 cut(s) 6, 147, 636, 1316, 1905, 1956, 2210, 2492, 2643
RsaNI GTAC 9 cut(s) 5, 146, 635, 1315, 1904, 1955, 2209, 2491, 2642
RseI CAYNNNNRTG 3 cut(s) 209, 645, 666
SacI GAGCTC 1 cut(s) 631
SalI GTCGAC 1 cut(s) 2344
SatI GCNGC 8 cut(s) 303, 519, 1266, 1443, 1967, 2151, 2676, 2679
Sau3AI GATC 8 cut(s) 111, 841, 1066, 1507, 1908, 2184, 2386, 2602
Sau96I GGNCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
SchI GAGTC 3 cut(s) 488, 860, 2305
ScrFI CCNGG 6 cut(s) 42, 1311, 1493, 1494, 1760, 2685
SduI GDGCHC 2 cut(s) 631, 1758
SfcI CTRYAG 3 cut(s) 1320, 1443, 2431
Sfr274I CTCGAG 2 cut(s) 630, 711
SfuI TTCGAA 1 cut(s) 1395
SinI GGWCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
SlaI CTCGAG 2 cut(s) 630, 711
SmaI CCCGGG 1 cut(s) 1494
SmiI ATTTAAAT 1 cut(s) 2064
SmiMI CAYNNNNRTG 3 cut(s) 209, 645, 666
SmlI CTYRAG 6 cut(s) 501, 630, 711, 1751, 2177, 2313
SmoI CTYRAG 6 cut(s) 501, 630, 711, 1751, 2177, 2313
SpeI ACTAGT 1 cut(s) 437
SphI GCATGC 1 cut(s) 242
SseBI AGGCCT 1 cut(s) 2445
SspI AATATT 1 cut(s) 723
SspMI CTAG 4 cut(s) 438, 1121, 2127, 2672
SstI GAGCTC 1 cut(s) 631
StuI AGGCCT 1 cut(s) 2445
StyD4I CCNGG 6 cut(s) 40, 1309, 1491, 1492, 1758, 2683
StyI CCWWGG 5 cut(s) 54, 210, 861, 2214, 2472
SwaI ATTTAAAT 1 cut(s) 2064
TaaI ACNGT 5 cut(s) 1015, 1173, 1649, 1954, 2201
TaiI ACGT 2 cut(s) 1601, 2310
TaqI TCGA 9 cut(s) 114, 333, 631, 712, 830, 1293, 1395, 1943, 2345
TaqII GACCGA 1 cut(s) 809
TatI WGTACW 4 cut(s) 1954, 2208, 2490, 2641
TfiI GAWTC 7 cut(s) 278, 406, 948, 1102, 1419, 1613, 2633
TscAI CASTG 5 cut(s) 186, 1041, 1654, 1933, 1984
TseFI GTSAC 3 cut(s) 491, 1088, 2308
TseI GCWGC 8 cut(s) 302, 518, 1265, 1442, 1966, 2150, 2675, 2678
Tsp45I GTSAC 3 cut(s) 491, 1088, 2308
TspGWI ACGGA 2 cut(s) 587, 833
TspMI CCCGGG 1 cut(s) 1492
TspRI CASTG 5 cut(s) 186, 1041, 1654, 1933, 1984
Van91I CCANNNNNTGG 1 cut(s) 2423
VpaK11BI GGWCC 7 cut(s) 356, 730, 753, 1781, 2222, 2268, 2414
XagI CCTNNNNNAGG 1 cut(s) 2664
XapI RAATTY 9 cut(s) 172, 678, 1130, 1150, 1382, 1391, 1521, 2065, 2400
XbaI TCTAGA 1 cut(s) 2126
XceI RCATGY 4 cut(s) 242, 2290, 2497, 2648
XcmI CCANNNNNNNNNTGG 1 cut(s) 2212
XhoI CTCGAG 2 cut(s) 630, 711
XmaI CCCGGG 1 cut(s) 1492
XmiI GTMKAC 3 cut(s) 606, 2345, 2430
XmnI GAANNNNTTC 4 cut(s) 71, 552, 947, 1289
XspI CTAG 4 cut(s) 438, 1121, 2127, 2672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.