Rroxscaffold_5G00357350

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001790
Physical Location & Seq
Forward (+)
36964002 .. 36968788
4787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00357350.1

Sequence Viewer

Length: 2451 bp
ATGGCTTCAGATGAGGAAATAGATAGCTTGTTCGAAAACGCGATGAAAGGACAATGGGAAAAGGTCATGGAGGCCTACAGAAATGGTATTACAGCTCAAGTAGCCAAGATAACCAGATCCAAGGAGACTGCTCTGCACATAGCTATAGCAGATGGCCGAACCGAAATCGTCCTAGAGTTGGTGAACATTATTCTCAATACTGAAGACGACAGTGCATCAACCGCAGTACTCAGCATAGCAAATGATAGAGGCAACACGCCTCTACATCTAGCTGCTTGGCTCGGGAATGTACAAGTGTGCCACAGCATTGCAGCCAAGGCGGCGAGCCTCGTCTCCGTTCGAAATGTGGAGGGCGAGACACCTCTCTTCTTGGCAGCCCTTCATGGTAACACAAAGGCTTTTCTTTGCCTTAATTTTCATTGCCAAGAAAAACCCCATTCCTCGAACAGAGACAACAATGGTGATACCATTCTTCATGCTGCAATCTCCGGTGAATACTTCAGTTTGGCATTTCAGATAATTTGGATGTACCCAGAACTTGTGAACTCTATCAATCAAAATGGTTCGTCTCCACTGCATATTCTAGCCAGTTTGATGGTTGAAGAACTCAAAGTGGAAGAATATAACCATGAAGCTTGTCTTTCTAAGGCAGGAGACAGAAATAGTTCTTCATACCCAGAAAACTATGAAACATGCATGAATTTCTTCCAAGTTCTCAGAAGTTTGTTTCAAGTGTTAAGTAAAAAGAGGGGAGATAGGTGGCACGTATACTGGCATAAGACGCACAGCTCACTCCTTCAAGCTAATGGAGGAAATGCGAAGGGTACTAATGGTCCTGTTGATGATGAAGAGAATCCCAAACAAGGAGCATCAACTTCTGCAGTGAGGTCACAATCAATAGATGTTACAAACGAAATATCAGAGGCAAAGTGTTCAAGATCAACCAATGGGGCAAATCATCCAGAGGGAAAGGGGAGAAAGAATCACCTGTTTCCTCCCAATTATGTCTCATTTGTTCTGTTCTTCAAGTTTCTGATGAAGACCTTGCTAATCATTCTGGGATTTGGAATTTGGGGGATAAGAAAAATCCAAGAAAAGAAAGAGAGACATATATGGGCAAATCAGGTCATGAATGCATTGGTTGACCATACTTCTTTATACAAATATCGAAATACTGGGCAAAATCCCCAAGAAACTCAATCCAGCAAAGATAAAGAAGAATATGAAGTGCCTAATCCTGCAATGCTAGAGCAAGCTTCACCCTTGCCATCTGATCATGCGGCACTTAACAGTAGAGATACAAGTGCTGAGACGACTATCTTGTCCTCAAACCAGTACAATTACAAGATAGGGAGTGATCAGAACAAACAAGGTGAGTTAACTTCTCCGCCCTTTCTTGTTTACACTGCATTCTTCATTGACATCACAAGACTTAGAGATTTCCACACTCATCATGCTACTCATTGCACAGAGAGGAAGAACAATAATCCCTTGGGAGGTAAGAAGAATGTAGTTCCTGGAAAGAATCAGTCACCTGTTTTAATTGCAGCAAAGATGGGAGTAACTGAAATGGTGGAGAAAATCCTAGACAAATTTCCAGTGGCCATCCAGGATGTTGACTCTGATAACAAGAATGTTATACTCTTAGCAGTTGAGAACAGGCAACCGCATGTGTACAATCTCCTTCAGAAGAGAAAGATACTAAAAGAAAGCCTGTTTCGTCAGTTGGACAACGAAGGTAATAGCGCATTACATCTTGCTGCTACATGTGGACAGTACCGGCCTTGGCTTATTCCAGGAGCGGCATTGCAAATGCAATGGGAAATCAAGTGGTATAAGTTTGTCAAAAAATCCATGCCGCATCGTTACTTTGTTCGCTACAACAATAGAGGCCAGACACCAAAGGAGATATTTAAAGATACACACAAACATCTTATAAAAGAAGGAAGCAAATGGCTAATTAAAACCTCGGAATCATGCTCCGTGGTTGCAGCCCTCATTGCAACGGTTGCATTTGCTACATCAGCAACTGTACCAGGAGGACTCGATCAGCTTACAGGCGAGCCAATTCTCAAAGACAAGACAGCATTCAGTGCCTTCACCATTTCATCACTAGTTGCTCTCTGCTTCTCAATAACTTCCATGGTCTTCTTTCTTTCAATCCTCACTTCTAGATATGAAGAAAGTGATTTTTCCATGGACTTGCCTCGGAAACTCTTGATCGGTTTGACATCACTCTTTGCATCTATAGCTTCTATGTTAGTCTCGTTTTGCACAGGTCATATCTTTCTCCTAAAACATCAACTAAGATATGTGGCATATTCATTGTATGCAGCAACTTGCTTGCCAGTTACATTTTTCGCACTTGCACAGCTGCCGCTCTACTTTGATCTATTGAAGGCCATCATCAGAAAGGTACCTCAACGGAGCTACGAGGTGTATCCACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001508 GO:0002027 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009893 GO:0009987 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042383 GO:0042391 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 PGG

Protein Analysis

816

Amino Acids

91.52

Weight (kDa)

8.48

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 39 - 107 1.3e-07 Ankyrin repeats (3 copies)
PGG PF13962 651 - 762 3.3e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1937
AasI GACNNNNNNGTC 1 cut(s) 1321
Acc65I GGTACC 1 cut(s) 2416
AccB1I GGYRCC 1 cut(s) 2416
AccBSI CCGCTC 2 cut(s) 1802, 2380
AccI GTMKAC 1 cut(s) 768
AccII CGCG 1 cut(s) 41
AciI CCGC 8 cut(s) 222, 320, 1280, 1388, 1667, 1802, 1859, 2378
AclWI GGATC 1 cut(s) 111
AcoI YGGCCR 2 cut(s) 154, 1602
AcsI RAATTY 3 cut(s) 700, 1068, 1592
AcuI CTGAAG 3 cut(s) 222, 484, 1670
AfaI GTAC 9 cut(s) 228, 291, 530, 826, 1337, 1676, 1778, 2034, 2418
AfiI CCNNNNNNNGG 3 cut(s) 178, 863, 1496
AflIII ACRYGT 1 cut(s) 1766
AgsI TTSAA 7 cut(s) 602, 731, 800, 936, 1027, 2160, 2398
AhlI ACTAGT 1 cut(s) 2113
AjnI CCWGG 4 cut(s) 1516, 1608, 1795, 2035
AlwI GGATC 1 cut(s) 111
AlwNI CAGNNNCTG 1 cut(s) 2030
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 6 cut(s) 72, 154, 1602, 1781, 1891, 2400
ApeKI GCWGC 9 cut(s) 272, 311, 374, 479, 1547, 1760, 1991, 2333, 2374
ApoI RAATTY 3 cut(s) 700, 1068, 1592
Asp700I GAANNNNTTC 2 cut(s) 664, 704
Asp718I GGTACC 1 cut(s) 2416
AspLEI GCGC 1 cut(s) 1748
AspS9I GGNCC 1 cut(s) 833
AsuHPI GGTGA 8 cut(s) 193, 473, 503, 977, 1251, 1385, 1524, 2092
AsuII TTCGAA 2 cut(s) 33, 340
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 833
BalI TGGCCA 1 cut(s) 1604
BanI GGYRCC 1 cut(s) 2416
BbsI GAAGAC 3 cut(s) 210, 1046, 2140
BbvI GCAGC 9 cut(s) 259, 323, 386, 466, 1559, 1747, 2003, 2345, 2361
BccI CCATC 6 cut(s) 146, 589, 1276, 1549, 1613, 2411
BciT130I CCWGG 4 cut(s) 1518, 1610, 1797, 2037
BciVI GTATCC 1 cut(s) 2451
BclI TGATCA 2 cut(s) 1273, 1357
BcuI ACTAGT 1 cut(s) 2113
BfaI CTAG 7 cut(s) 173, 269, 584, 1247, 1586, 2114, 2172
BfmI CTRYAG 4 cut(s) 76, 144, 879, 2247
BfuI GTATCC 1 cut(s) 2451
BglI GCCNNNNNGGC 1 cut(s) 320
BmcAI AGTACT 1 cut(s) 228
Bme1390I CCNGG 4 cut(s) 1518, 1610, 1797, 2037
Bme18I GGWCC 1 cut(s) 833
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 833
BmiI GGNNCC 1 cut(s) 2418
BmrFI CCNGG 4 cut(s) 1518, 1610, 1797, 2037
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 4 cut(s) 224, 878, 1870, 2252
BmuI ACTGGG 1 cut(s) 1185
BpiI GAAGAC 3 cut(s) 210, 1046, 2140
Bpu14I TTCGAA 2 cut(s) 33, 340
BpuEI CTTGAG 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 766
BsaJI CCNNGG 9 cut(s) 120, 315, 1491, 1784, 1968, 1983, 2142, 2196, 2207
BsaWI WCCGGW 1 cut(s) 488
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 863, 1496
Bse118I RCCGGY 1 cut(s) 1779
Bse1I ACTGG 6 cut(s) 588, 776, 1180, 1333, 1598, 2348
Bse3DI GCAATG 7 cut(s) 306, 418, 1248, 1462, 1805, 1823, 1998
BseBI CCWGG 4 cut(s) 1518, 1610, 1797, 2037
BseDI CCNNGG 9 cut(s) 120, 315, 1491, 1784, 1968, 1983, 2142, 2196, 2207
BseGI GGATG 4 cut(s) 531, 958, 1605, 1618
BseLI CCNNNNNNNGG 3 cut(s) 178, 863, 1496
BseMI GCAATG 7 cut(s) 306, 418, 1248, 1462, 1805, 1823, 1998
BseMII CTCAG 3 cut(s) 244, 730, 1299
BseNI ACTGG 6 cut(s) 588, 776, 1180, 1333, 1598, 2348
BseXI GCAGC 9 cut(s) 259, 323, 386, 466, 1559, 1747, 2003, 2345, 2361
BsgI GTGCAG 1 cut(s) 119
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 6 cut(s) 74, 156, 1604, 1783, 1893, 2402
BshNI GGYRCC 1 cut(s) 2416
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 489, 1780
BslI CCNNNNNNNGG 3 cut(s) 178, 863, 1496
BsmBI CGTCTC 3 cut(s) 337, 573, 1304
BsmI GAATGC 3 cut(s) 1138, 1409, 2087
BsnI GGCC 6 cut(s) 74, 156, 1604, 1783, 1893, 2402
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 2 cut(s) 33, 340
Bsp1407I TGTACA 2 cut(s) 289, 1674
Bsp143I GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
Bsp19I CCATGG 2 cut(s) 2142, 2196
BspACI CCGC 8 cut(s) 222, 320, 1280, 1388, 1667, 1802, 1859, 2378
BspANI GGCC 6 cut(s) 74, 156, 1604, 1783, 1893, 2402
BspCNI CTCAG 3 cut(s) 243, 729, 1300
BspFNI CGCG 1 cut(s) 41
BspHI TCATGA 1 cut(s) 1128
BspLI GGNNCC 1 cut(s) 2418
BspMAI CTGCAG 1 cut(s) 883
BspPI GGATC 1 cut(s) 111
BspT104I TTCGAA 2 cut(s) 33, 340
BspT107I GGYRCC 1 cut(s) 2416
BsrBI CCGCTC 2 cut(s) 1802, 2380
BsrDI GCAATG 7 cut(s) 306, 418, 1248, 1462, 1805, 1823, 1998
BsrFI RCCGGY 1 cut(s) 1779
BsrGI TGTACA 2 cut(s) 289, 1674
BsrI ACTGG 6 cut(s) 588, 776, 1180, 1333, 1598, 2348
BssAI RCCGGY 1 cut(s) 1779
BssECI CCNNGG 9 cut(s) 120, 315, 1491, 1784, 1968, 1983, 2142, 2196, 2207
BssMI GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
BssNAI GTATAC 1 cut(s) 769
BssT1I CCWWGG 6 cut(s) 120, 315, 1491, 1784, 2142, 2196
Bst1107I GTATAC 1 cut(s) 769
Bst2UI CCWGG 4 cut(s) 1518, 1610, 1797, 2037
Bst4CI ACNGT 5 cut(s) 212, 1292, 1776, 2008, 2032
Bst6I CTCTTC 3 cut(s) 371, 843, 1685
BstAPI GCANNNNNTGC 2 cut(s) 2009, 2093
BstAUI TGTACA 2 cut(s) 289, 1674
BstBAI YACGTR 1 cut(s) 766
BstBI TTCGAA 2 cut(s) 33, 340
BstC8I GCNNGC 4 cut(s) 325, 1254, 2063, 2345
BstDEI CTNAG 7 cut(s) 230, 645, 716, 1308, 1433, 1645, 2306
BstDSI CCRYGG 3 cut(s) 1983, 2142, 2196
BstF5I GGATG 4 cut(s) 531, 958, 1605, 1618
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 1748
BstKTI GATC 7 cut(s) 119, 941, 1276, 1360, 2050, 2223, 2392
BstMBI GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
BstNI CCWGG 4 cut(s) 1518, 1610, 1797, 2037
BstNSI RCATGY 3 cut(s) 696, 1673, 1770
BstSCI CCNGG 4 cut(s) 1516, 1608, 1795, 2035
BstSFI CTRYAG 4 cut(s) 76, 144, 879, 2247
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 9 cut(s) 259, 323, 386, 466, 1559, 1747, 2003, 2345, 2361
BstV2I GAAGAC 3 cut(s) 210, 1046, 2140
BstX2I RGATCY 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 595
BstYI RGATCY 1 cut(s) 116
BstZ17I GTATAC 1 cut(s) 769
BsuI GTATCC 1 cut(s) 2451
BsuRI GGCC 6 cut(s) 74, 156, 1604, 1783, 1893, 2402
BtgI CCRYGG 3 cut(s) 1983, 2142, 2196
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 4 cut(s) 531, 958, 1605, 1618
BtsI GCAGTG 3 cut(s) 572, 888, 1404
BtsIMutI CAGTG 6 cut(s) 217, 572, 888, 1404, 1605, 2098
Cac8I GCNNGC 4 cut(s) 325, 1254, 2063, 2345
CaiI CAGNNNCTG 1 cut(s) 2030
CciI TCATGA 1 cut(s) 1128
CfoI GCGC 1 cut(s) 1748
Cfr10I RCCGGY 1 cut(s) 1779
Cfr13I GGNCC 1 cut(s) 833
CseI GACGC 1 cut(s) 790
Csp6I GTAC 9 cut(s) 227, 290, 529, 825, 1336, 1675, 1777, 2033, 2417
CviQI GTAC 9 cut(s) 227, 290, 529, 825, 1336, 1675, 1777, 2033, 2417
DdeI CTNAG 7 cut(s) 230, 645, 716, 1308, 1433, 1645, 2306
DpnI GATC 7 cut(s) 118, 940, 1275, 1359, 2049, 2222, 2391
DpnII GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
DraI TTTAAA 1 cut(s) 1915
DrdI GACNNNNNNGTC 1 cut(s) 1321
DseDI GACNNNNNNGTC 1 cut(s) 1321
EaeI YGGCCR 2 cut(s) 154, 1602
Eam1104I CTCTTC 3 cut(s) 371, 843, 1685
EarI CTCTTC 3 cut(s) 371, 843, 1685
EciI GGCGGA 1 cut(s) 1377
Eco130I CCWWGG 6 cut(s) 120, 315, 1491, 1784, 2142, 2196
Eco147I AGGCCT 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 833
Eco57I CTGAAG 3 cut(s) 222, 484, 1670
Eco88I CYCGRG 1 cut(s) 281
EcoRII CCWGG 4 cut(s) 1516, 1608, 1795, 2035
EcoT14I CCWWGG 6 cut(s) 120, 315, 1491, 1784, 2142, 2196
EcoT22I ATGCAT 2 cut(s) 698, 1138
ErhI CCWWGG 6 cut(s) 120, 315, 1491, 1784, 2142, 2196
Esp3I CGTCTC 3 cut(s) 337, 573, 1304
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FbaI TGATCA 2 cut(s) 1273, 1357
FblI GTMKAC 1 cut(s) 768
FokI GGATG 4 cut(s) 538, 945, 1592, 1625
FspBI CTAG 7 cut(s) 173, 269, 584, 1247, 1586, 2114, 2172
GlaI GCGC 1 cut(s) 1747
HaeIII GGCC 6 cut(s) 74, 156, 1604, 1783, 1893, 2402
HapII CCGG 2 cut(s) 489, 1780
HgaI GACGC 1 cut(s) 790
HhaI GCGC 1 cut(s) 1748
Hin6I GCGC 1 cut(s) 1746
HinP1I GCGC 1 cut(s) 1746
HincII GTYRAC 3 cut(s) 1144, 1380, 1618
HindII GTYRAC 3 cut(s) 1144, 1380, 1618
HindIII AAGCTT 2 cut(s) 633, 1254
HinfI GANTC 6 cut(s) 853, 982, 1525, 1619, 1973, 2043
HpaI GTTAAC 1 cut(s) 1380
HpaII CCGG 2 cut(s) 489, 1780
HphI GGTGA 8 cut(s) 193, 473, 503, 977, 1251, 1385, 1524, 2092
Hpy166II GTNNAC 9 cut(s) 184, 544, 769, 1144, 1380, 1402, 1618, 1675, 1772
Hpy188III TCNNGA 6 cut(s) 283, 936, 962, 1129, 2172, 2218
Hpy8I GTNNAC 9 cut(s) 184, 544, 769, 1144, 1380, 1402, 1618, 1675, 1772
HpyAV CCTTC 8 cut(s) 389, 806, 814, 1694, 1730, 1937, 2107, 2392
HpyCH4III ACNGT 5 cut(s) 212, 1292, 1776, 2008, 2032
HpyCH4IV ACGT 1 cut(s) 765
HpyF3I CTNAG 7 cut(s) 230, 645, 716, 1308, 1433, 1645, 2306
HpySE526I ACGT 1 cut(s) 765
HspAI GCGC 1 cut(s) 1746
KpnI GGTACC 1 cut(s) 2420
Ksp22I TGATCA 2 cut(s) 1273, 1357
KspAI GTTAAC 1 cut(s) 1380
Kzo9I GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
LmnI GCTCC 4 cut(s) 866, 1799, 1985, 2427
Lsp1109I GCAGC 9 cut(s) 259, 323, 386, 466, 1559, 1747, 2003, 2345, 2361
LweI GCATC 4 cut(s) 224, 878, 1870, 2252
MaeI CTAG 7 cut(s) 173, 269, 584, 1247, 1586, 2114, 2172
MaeII ACGT 1 cut(s) 765
MaeIII GTNAC 7 cut(s) 386, 888, 904, 1530, 1561, 1865, 2350
MalI GATC 7 cut(s) 118, 940, 1275, 1359, 2049, 2222, 2391
MbiI CCGCTC 2 cut(s) 1802, 2380
MboI GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
MflI RGATCY 1 cut(s) 116
MlsI TGGCCA 1 cut(s) 1604
MluNI TGGCCA 1 cut(s) 1604
MlyI GAGTC 2 cut(s) 1613, 2037
MmeI TCCRAC 1 cut(s) 1707
Mox20I TGGCCA 1 cut(s) 1604
Mph1103I ATGCAT 2 cut(s) 698, 1138
MroXI GAANNNNTTC 2 cut(s) 664, 704
MscI TGGCCA 1 cut(s) 1604
MseI TTAA 7 cut(s) 411, 737, 1287, 1379, 1541, 1914, 1962
Msp20I TGGCCA 1 cut(s) 1604
MspA1I CMGCKG 1 cut(s) 2374
MspI CCGG 2 cut(s) 489, 1780
MspR9I CCNGG 4 cut(s) 1518, 1610, 1797, 2037
Mva1269I GAATGC 3 cut(s) 1138, 1409, 2087
MvaI CCWGG 4 cut(s) 1518, 1610, 1797, 2037
MvnI CGCG 1 cut(s) 41
NcoI CCATGG 2 cut(s) 2142, 2196
NdeII GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
NlaIV GGNNCC 1 cut(s) 2418
NmuCI GTSAC 2 cut(s) 888, 1530
NsiI ATGCAT 2 cut(s) 698, 1138
NspI RCATGY 3 cut(s) 696, 1673, 1770
NspV TTCGAA 2 cut(s) 33, 340
PagI TCATGA 1 cut(s) 1128
PceI AGGCCT 1 cut(s) 74
PciI ACATGT 1 cut(s) 1766
PctI GAATGC 3 cut(s) 1138, 1409, 2087
PdmI GAANNNNTTC 2 cut(s) 664, 704
PfeI GAWTC 4 cut(s) 853, 982, 1525, 1973
PfoI TCCNGGA 3 cut(s) 1516, 1608, 1795
PleI GAGTC 2 cut(s) 1613, 2037
PpsI GAGTC 2 cut(s) 1613, 2037
Ppu21I YACGTR 1 cut(s) 766
PscI ACATGT 1 cut(s) 1766
PsiI TTATAA 1 cut(s) 1937
Psp6I CCWGG 4 cut(s) 1516, 1608, 1795, 2035
PspGI CCWGG 4 cut(s) 1516, 1608, 1795, 2035
PspN4I GGNNCC 1 cut(s) 2418
PspPI GGNCC 1 cut(s) 833
PstI CTGCAG 1 cut(s) 883
PstNI CAGNNNCTG 1 cut(s) 2030
PsuI RGATCY 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 2374
RsaI GTAC 9 cut(s) 228, 291, 530, 826, 1337, 1676, 1778, 2034, 2418
RsaNI GTAC 9 cut(s) 227, 290, 529, 825, 1336, 1675, 1777, 2033, 2417
SaqAI TTAA 7 cut(s) 411, 737, 1287, 1379, 1541, 1914, 1962
Sau3AI GATC 7 cut(s) 116, 938, 1273, 1357, 2047, 2220, 2389
Sau96I GGNCC 1 cut(s) 833
ScaI AGTACT 1 cut(s) 228
SchI GAGTC 2 cut(s) 1613, 2037
ScrFI CCNGG 4 cut(s) 1518, 1610, 1797, 2037
SfaNI GCATC 4 cut(s) 224, 878, 1870, 2252
SfcI CTRYAG 4 cut(s) 76, 144, 879, 2247
SfuI TTCGAA 2 cut(s) 33, 340
SinI GGWCC 1 cut(s) 833
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
SpeI ACTAGT 1 cut(s) 2113
SseBI AGGCCT 1 cut(s) 74
SsiI CCGC 8 cut(s) 222, 320, 1280, 1388, 1667, 1802, 1859, 2378
SspMI CTAG 7 cut(s) 173, 269, 584, 1247, 1586, 2114, 2172
StuI AGGCCT 1 cut(s) 74
StyD4I CCNGG 4 cut(s) 1516, 1608, 1795, 2035
StyI CCWWGG 6 cut(s) 120, 315, 1491, 1784, 2142, 2196
TaaI ACNGT 5 cut(s) 212, 1292, 1776, 2008, 2032
TaiI ACGT 1 cut(s) 768
TaqI TCGA 5 cut(s) 33, 340, 443, 1168, 2046
TatI WGTACW 4 cut(s) 226, 289, 1335, 1674
TauI GCSGC 5 cut(s) 323, 1283, 1805, 1861, 2380
TfiI GAWTC 4 cut(s) 853, 982, 1525, 1973
Tru1I TTAA 7 cut(s) 411, 737, 1287, 1379, 1541, 1914, 1962
Tru9I TTAA 7 cut(s) 411, 737, 1287, 1379, 1541, 1914, 1962
TscAI CASTG 6 cut(s) 217, 579, 888, 1411, 1605, 2098
TseFI GTSAC 2 cut(s) 888, 1530
TseI GCWGC 9 cut(s) 272, 311, 374, 479, 1547, 1760, 1991, 2333, 2374
Tsp45I GTSAC 2 cut(s) 888, 1530
TspGWI ACGGA 3 cut(s) 325, 1972, 2440
TspRI CASTG 6 cut(s) 217, 579, 888, 1411, 1605, 2098
VpaK11BI GGWCC 1 cut(s) 833
XapI RAATTY 3 cut(s) 700, 1068, 1592
XbaI TCTAGA 1 cut(s) 2171
XceI RCATGY 3 cut(s) 696, 1673, 1770
XmiI GTMKAC 1 cut(s) 768
XmnI GAANNNNTTC 2 cut(s) 664, 704
XspI CTAG 7 cut(s) 173, 269, 584, 1247, 1586, 2114, 2172
ZrmI AGTACT 1 cut(s) 228
Zsp2I ATGCAT 2 cut(s) 698, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.