Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002438

Physical Location & Seq

Genomic Coordinates Forward (+)

25856902 .. 25885229

Gene Structure 28328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1164800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5898 bp

ATGGCGAGGGTTTACAACAATTGGGAGCGGCTGGTGCGGGCCGCGCTCAACCGAGAGCAGCTCAGGAGCTCCGGCCAGGGCCACGGGAGGGTTCCTAGTGGAATCGCCGGCGCCGTCCCGCCGTCGCTCGGGAAGACCACCAATATCGATGCTATCTTACAAGCTGCAGATGAGATTCAAGCTGAGGATCCCAACGTCTCCAGAATTCTGTGTGAGCAGGCGTATACCATGGCGCAAAATCTGGACCCGAAAAGTGACGGCAGAGGTGTTCTTCAGTTTAAGACTGGATTGATGTCTGTAATCAAGCAAAAATTTGCAAAAAAAGATGGGGGTCAAATAGATCGGAATCGTGATATTGAGCACCTCTGGGATTTTTACCAGCGCTACAAGCGACGACATAGAGTTGATGATATCCAGAGACAGGAACAAATATGGCGTGAATCTGGAACTTTTGGTGCTGACTTTGGCGATTATTTAGAAATGAAAAAGACAATTGCTACCTTGAGGGCTTTAGTTGAGGTGATGGAGGCACTTAGCAAAGTTGCTGATCCTAATGGAGTTGGAAGACTTATTACAGAGGAGTTGCGCAAAGTTAAAAGCACCGATGCAACATTATCTGGGGAGCTTACAGCCTACAATATTGTCCCTCTAGAAGCACCATCATTGACCAATGCAATTGGAGGTTTCCCAGAAGTTAGAGGTGCAATATCTACACTTAGGTATACTGAGCAATTCCCCAGACTTCCTGCTGACTTTGAGATATCCGGACAACGGAATGCAGACATGTTTGATCTTCTGGAATGTGTTTTTGGTTTTCAGAAAGACAATGTGAGGAACCAGAGAGAGAATGTTGTTCTTACAATTGCAAATGCACAATCTCGGCTTGGTATACCCGTTGAGGCTTATCCGAAAATTGATGAGAAAGCAATAAATGAGGTATTTCTGAAAGTTCTGGATAATTATATGAAATGGTGCCGATACCTGCGGATACGCCTTGCATGGAATAGTTTAGAAGCAATTGATCGGGACAGGAAACTTTTTCTGGTTTCCTTGTACTTTCTGATTTGGGGTGAAGCTGCAAATGTCCGCTTTCTTCCTGAATGTATTTGCTACATATTTCACCATATGGCTAAAGAGTTAGATGCAATACTAGATCATGGAGAAGCTATTCTTGCTGCCAGCTGCAGAACCGAGTCTGGGTCTGTGTCCTTCCTTCAGCAAATCATTTTTCCAATATATGATATATTGGCTGCGGAAGCCGCCAGAAATAATAATGGGAAAGCTGCGCATTCAGCATGGAGGAATTATGATGACTTTAATGAATATTTTTGGTCACCTGCTTGTTTTGAATTGAAGTGGCCTATGAGGAGGGATTCACCATTCTTGTTCAAACCTAAAAAGCGAAAAAGGACCGGAAAAAGCACTTTCGTTGAGCATCGGACCTTTTTTCACTTGTATAGAAGTTTCCATCGCCTGTGGATCTTTTTAGCTTTGATGTTCCAGGCTTTGGCAATTATAGCCTTTAATGGACACATCAACTTGGATACTTTAAAGACAGTCCTCAGCATTGGACCAACTTTTGTTATCATGAACTTCATAGAGAGTTTTCTGGACGTGGTGCTCATGTTTGGGGCTTACACCACAGCCAGAGGCATGGCTGTCTCTAGACTTGTAATTAGATTTTTCTGGTTTGGCTTGAGCTCTGTTGCTGTGACATATATCTATCTGAAAGTTCTGCATGAAAGAAATGACAGAAACTCAGATTCATTCTACTTTCGGATATATGTTATTGTATTGGGTGTTTATGCTGCTCTGCGCATAGTCCTTGCACTATTGCTTAAGTTTCCAGGATGTCATAAAATTTCTGAGATGTCTGATCAATCCTTCTTCCAATTTTTTAAGTGGATTTACCAGGCAAGTCCTACTTTTCTCGGTTTGAACTTTGAAATGGAGCGTTACTTTGTTGGACGCGGTCTTTATGAGAAGATGAGTGATTACTGCAGGTGTGCTGATATATTTGTCGCCACCCTAAGCCATTTCAAGTTCGTTAGGGTTATTGTATTCCTTTTCACTTTATATTTGTCTCTTGGTTTTGGAGTACTCAAATTTGGACTCTGTGATGAAGATTTCTGTTGGAACTCCGGCCAATATATTTTGATGGCCTGGTTCAAAATATTCATTTTTGGCTATGTCTGCATGTCAAATCGAGTGTTGACATATATGCTGATTAAACCTCTTGTTGAACCCACCAAAATCATCGTGGACCTTCCATCTCTGCAATACTCATGGCATGATTTGGTGTCAAAGAATAACAATAATGCATTGACAGTAGCTTGCCTATGGGCTCCTGTTGTGGGTATCTATCTCATGGATTTTTACATATGGTACACAATCCTGTCAGCCATTATTGGTGGTGTGATCGGTGCAAGAGCTCGGTTAGGCGAGATACGTTCGATTGAAATGGTGCATAAACGCTTTGAGAGTTTTCCAGAAGCTTTTGCCAAGAATCTAGTCTCACAAAATAGAAGGTTGCCGTTTAACAGTCAGTCATCCCAGGATTCTCAAGATTCAAACAAGATGTATGCAGCCATGTTTTCTCCATTTTGGAACGAGATAATTAAAAGTTTGCGAGAGGAGGATTTTATTAGTAATAGGGAGATGGATTTGCTCTGTATGCCTAGCAACACAGGAAGTCTAAGATTAGTTCAATGGCCTTTGTTTCTTCTGAGCAGTAAGATCTTGCTGGCAATTGATTTGGCTTTGGACTGCAAAGATACACAGGCAGATCTTTGGAATAGAATATGCAGGGATGAGTACATGGCATATGCAGTTCAGGAAGTCTACTACAGTATTGAAAAGATACTATATTCTCTGGTTGATGGTGAAGGGAGACTTTGGGTTGAAAGGATCTACCGCGAGATCAACAACAGTATTATAGAGGGTTCTCTTCTTGTAACTTTAATTCTTAAGAAGCTTCCCCAGGTGTTACAGAAACTTACAGCTTTAACTGGGCTGCTGATACGCAATGAAACTGATATACTTGCAAAAGGTGCTGCTAAATCTGTATTTGAGCTGTATGATGTTGTTACTCATGACCTTTTGTCATCTGATTTGAGGGAGCAGCTTGACACTTGGAGCCTTTTAGCAAAAGCAAGAAATGAAGGGCGCTTATTTTCCAGGATAAAGTGGCCCAATGATCCAGAAACTAAAGAGCTGGTTAAGCGGTTACACCTTCTTGTCACTGTAAAGGATTCTGCAGCAAATATTCCAAAAAATCTTGAAGCAAGAAGAAGATTAGAGTTTTTTACAAATTCATTGTTTATGGACATGCCTTTAGCAAAGCCAGTTAGTGAAATGGTTCCTTTCTGCGTCTTCACCCCGTACTACAGTGAGACGGTACTTTATAGTTCCTCCGAATTGCGAGTTGAAAATGAGGATGGAATATCAATTCTTTTCTATCTTCAGAAAATTTTTCCAGATGAGTGGGACAACTTTTTGGAACGGATTGGTCGGGGTGAGTCTACTGGGGATGCTGAGCTGCAGGAAAATTCAAGTGATTCTTTGGAGCTTCGGTTTTGGGTATCTTATCGCGGGCAGACTTTAGCCAGGACCGTGCGTGGTATGATGTATTATAGAAGGGCTTTAATGCTACAGAGTTTTATGGAAAGAAGATCGTTTGGAGTGGGCGATTATCCGCAAAATGATGTCTTCACTAGTCAAGGTTATGAGTTGTCACGTGAATCACGTGCACAAGCGGATTTGAAATTTACTTATGTGGTGTCATGCCAAATTTATGGGCAACAAAAGCAGAGAAAGGCACCGGAGGCTGCTGATATATCCCTTTTGTTGCAGAGGAATGAAGCCCTTCGGGTTGCATATATACACGTTGAGGAGAGTGGTGCAAGTGAAGGAAAGACCACAAAGGAATTTTATTCAAAGCTTGTAAAAGCCGATATACATGGAAAGGATCAGGAAATATACTCCATTAAACTTCCTGGTGATCCGAAGCTTGGTGAAGGGAAGCCTGAAAATCAAAACCATGCAATAGTGTTCACTCGTGGGGAAGCTGTTCAGACTATTGACATGAACCAGGACAACTACCTTGAGGAGGCAATGAAAATGAGAAATCTCCTTGAGGAATTTCGCAGAAAACATGGCATTCGACATCCAACTATTCTTGGTGTGCGAGAGCACGTATTTACTGGAAGTGTTTCATCTTTAGCATGGTTCATGTCCAATCAAGAGACTAGCTTTGTGACTTTGGCTCAACGAGTTTTAGCATATCCTCTCAAAGTTCGCATGCACTATGGACACCCTGATGTGTTTGACCGAATTTTCCACACTACCCGAGGTGGGATTAGTAAATCGTCACGTGTTATCAATATTAGTGAAGATATTTTTGGAGGGTTCAACTCCACATTGAGGCAGGGTAACATTACACACCACGAATACATTCAGGTTGGGAAAGGAAGAGACGTCGGGTTAAATCAGATTGCATTATTTGAAGGGAAAGTAGCAGGAGGAAATGGGGAGCAAGTCCTAAGTAGGGATGTGTACAGGATTGGGCAACTTTTTGATTTTTTCAGAATGTTCTCTTTCTTTTACACCACTGTTGGTTTCTACGTCTGCACAATGATGACTGTTCTCATAGTATACATATTCTTATACGGAAGGGCTTATCTGGCTTTCTCTGGAATTGATCGTGCAATTGCGAAGCAAGCAAAATTGTCGGGTAACACAGCATTAGATGCTGTTCTCAATGCACAATTCTTGGTCCAGATTGGAATTTTTACTGCAGTTCCAATGATTATGGGTTTTATACTTGAGTTGGGATTGTTGAAGGCGGTTTTCAGTTTTATCACAATGCAGCTTCAGTTATGTTCTGTGTTCTTCACGTTCTCATTGGGAACTAAAACTCATTATTTTGGGCGCACCATTCTCCATGGAGGTGCAAAATACCGAGCAACTGGTAGAGGCTTTGTTGTCAAGCACATAAAGTTTGCTGAGAATTATAGGCTCTACTCGAGGAGTCATTTTGTCAAAGCGTTTGAAGTGGCTCTACTTCTCATAGTTTACATTGCATATGGGTACACAGATGGTGGTGCTGTTTCTTACATTTTGCTAACTCTCAGCAGTTGGTTCCTTGTCATCTCCTGGTTGTTTGCTCCCTATATCTTTAATCCGTCTGGTTTTGAGTGGCAAAAGACGGTGGAAGATTTTGATGATTGGACCGGTTGGCTTCTTTACCGAGGCGGAGTAGGAGTTAAGGGAGAGCATAGTTGGGAATCTTGGTGGGATGAGGAGCAGATGCATATTCAAACACTGAGAGGTCGTATATTGGAAACAATTTTGAGCTTGAGATTCTTCATGTTTCAATACGGCGTTGTCTACAAACTCCATATCACTGCAAAGGACACATCACTTGCGATTTACGGCTACTCTTGGGTTGTATTGGTGACTGTTGTCCTGATTTTCAAGGTCTTCAGCTTCAGTCCCAAAAAGTCCGCCAACTTTCAGCTCGTGATGAGATTTATACAGGGTGTGGCATCCTTAGGACTTGTCGCGGTTATTGCTCTTCTTGTAATATTTACCGACCTCTCAATAGTTGACCTGTTTGCAAGCCTGCTCGCAATCATTCCTACCGGATGGGCCATATTATGCCTAGCGGTCACTTGGAAGAGCATCGTCAGGCGTCTAGGTTTATGGGACTCGGTGAGGGAGTTTGCCAGGATGTACGATGCTGGTATGGGGATGCTCATTTTCGCTCCTATAGCGTTCTTGTCCTGGTTCCCTTTCATCTCCACCTTCCAGTCTCGACTTCTCTTTAACCAAGCATTTAGCCGTGGGTTGGAAATATCGCTCATCCTCGCCGGAAACAAAGCCAATGTTGACGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005911 GO:0007049 GO:0007275 GO:0008150 GO:0009506 GO:0009555 GO:0009556 GO:0009987 GO:0012505 GO:0016020 GO:0019953 GO:0022402 GO:0022414 GO:0030054 GO:0032501 GO:0032502 GO:0034293 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0048229 GO:0048236 GO:0048589 GO:0048856 GO:0048869 GO:0051321 GO:0051704 GO:0055044 GO:0071944 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase

Protein Analysis

1966

Amino Acids

225.33

Weight (kDa)

8.74

Isoelectric Point (pI)

39.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	345 - 454	1.1e-40	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	905 - 1082	7.5e-46	Callose synthase 1 domain
Glucan_synthase	PF02364	1089 - 1864	3.1e-230	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36850
fragaria_vesca	FvH4_5g07240
malus_domestica	MD06G1158200.v1.1 MD14G1164600.v1.1 MD14G1164700.v1.1 MD14G1164800.v1.1
prunus_persica	Prupe.5G158100_v2.0.a1 Prupe.5G158100_v2.0.a1 Prupe.5G181200_v2.0.a1
pyrus_communis	pycom06g14110 pycom06g14120 pycom06g14130 pycom14g13740
rosa_chinensis	RchiOBHm_Chr3g0493651 RchiOBHm_Chr7g0191411 RchiOBHm_Chr7g0191641
rosa_laevigata	RLG00000004437 RLG00000004438 RLG00000004457
rosa_multiflora	Rmu_sc0001123.1_g000007 Rmu_sc0002661.1_g000007 Rmu_sc0003634.1_g000001 Rmu_sc0003634.1_g000002
rosa_roxburghii	Rroxscaffold_157G00438350 Rroxscaffold_157G00438470 Rroxscaffold_2G00133560 Rroxscaffold_3G00263680 Rroxscaffold_3G00263840
rosa_rugosa	Rorug06G0510400 Rorug06G0510600
rosa_samantha	Rh7AG118000 Rh7AG119400 Rh7AG119500 Rh7BG120100 Rh7BG121600 Rh7BG121700 Rh7CG122900 Rh7CG124400 Rh7DG121000 Rh7DG122500
rosa_wichuraiana	Rw7G010190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1345, 1992
AatII	GACGTC	2 cut(s)	4479, 5895
Acc16I	TGCGCA	3 cut(s)	587, 1287, 1817
Acc36I	ACCTGC	3 cut(s)	990, 1345, 1992
AccB1I	GGYRCC	3 cut(s)	110, 972, 3817
AccB7I	CCANNNNNTGG	1 cut(s)	1507
AccBSI	CCGCTC	1 cut(s)	28
AccI	GTMKAC	7 cut(s)	224, 722, 889, 2841, 3521, 4653, 5388
AccII	CGCG	5 cut(s)	44, 1971, 2916, 3591, 5564
AccIII	TCCGGA	1 cut(s)	764
AclWI	GGATC	8 cut(s)	182, 195, 542, 1487, 2915, 3191, 3975, 3995
AcoI	YGGCCR	2 cut(s)	73, 2143
AcuI	CTGAAG	6 cut(s)	257, 1199, 3446, 4856, 5467, 5473
AcvI	CACGTG	3 cut(s)	3737, 3746, 4373
AcyI	GRCGYC	4 cut(s)	111, 4476, 5692, 5892
AfaI	GTAC	9 cut(s)	1055, 2100, 2387, 2816, 3383, 3399, 4556, 5090, 5735
AfeI	AGCGCT	1 cut(s)	383
AflII	CTTAAG	2 cut(s)	1838, 2966
AflIII	ACRYGT	3 cut(s)	783, 3883, 4372
AgeI	ACCGGT	1 cut(s)	5231
AhdI	GACNNNNNGTC	1 cut(s)	3100
AhlI	ACTAGT	1 cut(s)	3713
AjiI	CACGTC	1 cut(s)	1615
AjuI	GAANNNNNNNTTGG	4 cut(s)	792, 824, 4383, 4415
Alw21I	GWGCWC	7 cut(s)	71, 363, 1623, 1703, 2434, 3751, 4194
Alw44I	GTGCAC	1 cut(s)	3747
AlwI	GGATC	8 cut(s)	182, 195, 542, 1487, 2915, 3191, 3975, 3995
Ama87I	CYCGRG	3 cut(s)	128, 4347, 5023
Aor13HI	TCCGGA	1 cut(s)	764
Aor51HI	AGCGCT	1 cut(s)	383
AoxI	GGCC	9 cut(s)	39, 73, 79, 1358, 2143, 2160, 2711, 3188, 5649
ApaLI	GTGCAC	1 cut(s)	3747
AsiGI	ACCGGT	1 cut(s)	5231
Asp700I	GAANNNNTTC	6 cut(s)	1167, 3357, 3864, 4068, 4210, 4452
AspLEI	GCGC	9 cut(s)	46, 113, 235, 384, 588, 1288, 1818, 3168, 4932
AvaI	CYCGRG	3 cut(s)	128, 4347, 5023
AvaII	GGWCC	8 cut(s)	244, 1410, 1440, 1571, 2263, 3609, 4774, 5229
AxyI	CCTNAGG	1 cut(s)	5551
BaeGI	GKGCMC	1 cut(s)	3751
BamHI	GGATCC	1 cut(s)	187
BanI	GGYRCC	3 cut(s)	110, 972, 3817
BanII	GRGCYC	4 cut(s)	71, 1703, 2347, 2434
BarI	GAAGNNNNNNTAC	4 cut(s)	556, 588, 5043, 5075
BauI	CACGAG	2 cut(s)	4056, 5519
BbrPI	CACGTG	3 cut(s)	3737, 3746, 4373
BbsI	GAAGAC	5 cut(s)	140, 571, 3364, 3700, 5473
Bbv12I	GWGCWC	7 cut(s)	71, 363, 1623, 1703, 2434, 3751, 4194
BbvCI	CCTCAGC	2 cut(s)	183, 1562
BceAI	ACGGC	7 cut(s)	98, 106, 274, 2518, 5395, 5449, 5826
BcgI	CGANNNNNNTGC	2 cut(s)	4710, 4744
BciVI	GTATCC	2 cut(s)	981, 1537
BclI	TGATCA	1 cut(s)	1876
BcuI	ACTAGT	1 cut(s)	3713
BfoI	RGCGCY	3 cut(s)	114, 385, 3169
BfrI	CTTAAG	2 cut(s)	1838, 2966
BfuAI	ACCTGC	3 cut(s)	990, 1345, 1992
BfuI	GTATCC	2 cut(s)	981, 1537
BglII	AGATCT	2 cut(s)	2736, 2785
BlpI	GCTNAGC	1 cut(s)	3534
BmcAI	AGTACT	1 cut(s)	2100
Bme18I	GGWCC	8 cut(s)	244, 1410, 1440, 1571, 2263, 3609, 4774, 5229
BmeRI	GACNNNNNGTC	1 cut(s)	3100
BmeT110I	CYCGRG	3 cut(s)	128, 4347, 5023
BmgBI	CACGTC	1 cut(s)	1615
BmrI	ACTGGG	2 cut(s)	3018, 3534
BmuI	ACTGGG	2 cut(s)	3018, 3534
BoxI	GACNNNNGTC	1 cut(s)	5462
BpiI	GAAGAC	5 cut(s)	140, 571, 3364, 3700, 5473
BplI	GAGNNNNNCTC	2 cut(s)	45, 77
BpmI	CTGGAG	1 cut(s)	184
Bpu10I	CCTNAGC	4 cut(s)	62, 183, 1562, 2030
Bpu1102I	GCTNAGC	1 cut(s)	3534
BpuEI	CTTGAG	7 cut(s)	523, 1717, 2547, 4124, 4154, 4844, 5377
Bsa29I	ATCGAT	1 cut(s)	147
BsaAI	YACGTR	4 cut(s)	3737, 3746, 4195, 4373
BsaBI	GATNNNNATC	1 cut(s)	345
BsaHI	GRCGYC	4 cut(s)	111, 4476, 5692, 5892
BsaJI	CCNNGG	9 cut(s)	76, 82, 228, 2553, 2979, 4348, 4942, 5248, 5842
BsaWI	WCCGGW	5 cut(s)	764, 1412, 3820, 5231, 5642
BsaXI	ACNNNNNCTCC	4 cut(s)	3557, 3587, 5247, 5277
Bse118I	RCCGGY	2 cut(s)	107, 5231
Bse1I	ACTGG	7 cut(s)	289, 3013, 3344, 3529, 4207, 4972, 5809
Bse21I	CCTNAGG	1 cut(s)	5551
Bse3DI	GCAATG	3 cut(s)	3031, 4119, 5076
Bse8I	GATNNNNATC	1 cut(s)	345
BseAI	TCCGGA	1 cut(s)	764
BseCI	ATCGAT	1 cut(s)	147
BseDI	CCNNGG	9 cut(s)	76, 82, 228, 2553, 2979, 4348, 4942, 5248, 5842
BseJI	GATNNNNATC	1 cut(s)	345
BseMI	GCAATG	3 cut(s)	3031, 4119, 5076
BseNI	ACTGG	7 cut(s)	289, 3013, 3344, 3529, 4207, 4972, 5809
BseRI	GAGGAG	7 cut(s)	593, 1381, 2648, 3905, 4121, 5041, 5315
BseSI	GKGCMC	1 cut(s)	3751
BsgI	GTGCAG	1 cut(s)	4612
Bsh1236I	CGCG	5 cut(s)	44, 1971, 2916, 3591, 5564
BshFI	GGCC	9 cut(s)	41, 75, 81, 1360, 2145, 2162, 2713, 3190, 5651
BshNI	GGYRCC	3 cut(s)	110, 972, 3817
BshTI	ACCGGT	1 cut(s)	5231
BshVI	ATCGAT	1 cut(s)	147
BsiHKAI	GWGCWC	7 cut(s)	71, 363, 1623, 1703, 2434, 3751, 4194
BsiHKCI	CYCGRG	3 cut(s)	128, 4347, 5023
BsiSI	CCGG	9 cut(s)	72, 108, 765, 1413, 2142, 3821, 5232, 5643, 5871
BslFI	GGGAC	6 cut(s)	101, 629, 1040, 3500, 5478, 5720
BsmBI	CGTCTC	3 cut(s)	202, 3386, 4467
BsmFI	GGGAC	6 cut(s)	101, 629, 1040, 3500, 5478, 5720
BsmI	GAATGC	3 cut(s)	781, 1288, 4158
BsnI	GGCC	9 cut(s)	41, 75, 81, 1360, 2145, 2162, 2713, 3190, 5651
BsoBI	CYCGRG	3 cut(s)	128, 4347, 5023
Bsp1286I	GDGCHC	8 cut(s)	71, 363, 1623, 1703, 2347, 2434, 3751, 4194
Bsp13I	TCCGGA	1 cut(s)	764
Bsp1407I	TGTACA	1 cut(s)	4554
Bsp1720I	GCTNAGC	1 cut(s)	3534
Bsp19I	CCATGG	2 cut(s)	228, 4942
BspANI	GGCC	9 cut(s)	41, 75, 81, 1360, 2145, 2162, 2713, 3190, 5651
BspDI	ATCGAT	1 cut(s)	147
BspEI	TCCGGA	1 cut(s)	764
BspFNI	CGCG	5 cut(s)	44, 1971, 2916, 3591, 5564
BspHI	TCATGA	2 cut(s)	1587, 3091
BspMAI	CTGCAG	6 cut(s)	169, 1187, 2003, 3259, 3543, 4798
BspMI	ACCTGC	3 cut(s)	990, 1345, 1992
BspPI	GGATC	8 cut(s)	182, 195, 542, 1487, 2915, 3191, 3975, 3995
BspQI	GCTCTTC	2 cut(s)	5580, 5672
BspT107I	GGYRCC	3 cut(s)	110, 972, 3817
BspTI	CTTAAG	2 cut(s)	1838, 2966
BsrBI	CCGCTC	1 cut(s)	28
BsrDI	GCAATG	3 cut(s)	3031, 4119, 5076
BsrFI	RCCGGY	2 cut(s)	107, 5231
BsrGI	TGTACA	1 cut(s)	4554
BsrI	ACTGG	7 cut(s)	289, 3013, 3344, 3529, 4207, 4972, 5809
BssAI	RCCGGY	2 cut(s)	107, 5231
BssECI	CCNNGG	9 cut(s)	76, 82, 228, 2553, 2979, 4348, 4942, 5248, 5842
BssNAI	GTATAC	4 cut(s)	225, 723, 890, 4654
BssNI	GRCGYC	4 cut(s)	111, 4476, 5692, 5892
BssSI	CACGAG	2 cut(s)	4056, 5519
BssT1I	CCWWGG	2 cut(s)	228, 4942
Bst1107I	GTATAC	4 cut(s)	225, 723, 890, 4654
Bst2BI	CACGAG	2 cut(s)	4056, 5519
Bst6I	CTCTTC	4 cut(s)	2952, 4465, 5580, 5672
BstACI	GRCGYC	4 cut(s)	111, 4476, 5692, 5892
BstAFI	CTTAAG	2 cut(s)	1838, 2966
BstAPI	GCANNNNNTGC	2 cut(s)	3050, 4748
BstAUI	TGTACA	1 cut(s)	4554
BstBAI	YACGTR	4 cut(s)	3737, 3746, 4195, 4373
BstDSI	CCRYGG	4 cut(s)	82, 228, 4942, 5842
BstEII	GGTNACC	1 cut(s)	1332
BstENI	CCTNNNNNAGG	1 cut(s)	4106
BstFNI	CGCG	5 cut(s)	44, 1971, 2916, 3591, 5564
BstH2I	RGCGCY	3 cut(s)	114, 385, 3169
BstHHI	GCGC	9 cut(s)	46, 113, 235, 384, 588, 1288, 1818, 3168, 4932
BstNSI	RCATGY	4 cut(s)	787, 2200, 3331, 4303
BstPAI	GACNNNNGTC	1 cut(s)	5462
BstPI	GGTNACC	1 cut(s)	1332
BstSLI	GKGCMC	1 cut(s)	3751
BstUI	CGCG	5 cut(s)	44, 1971, 2916, 3591, 5564
BstV2I	GAAGAC	5 cut(s)	140, 571, 3364, 3700, 5473
BstX2I	RGATCY	5 cut(s)	187, 1479, 2736, 2785, 2907
BstXI	CCANNNNNNTGG	3 cut(s)	1654, 3483, 3794
BstYI	RGATCY	5 cut(s)	187, 1479, 2736, 2785, 2907
BstZ17I	GTATAC	4 cut(s)	225, 723, 890, 4654
Bsu15I	ATCGAT	1 cut(s)	147
Bsu36I	CCTNAGG	1 cut(s)	5551
BsuI	GTATCC	2 cut(s)	981, 1537
BsuRI	GGCC	9 cut(s)	41, 75, 81, 1360, 2145, 2162, 2713, 3190, 5651
BsuTUI	ATCGAT	1 cut(s)	147
BtgI	CCRYGG	4 cut(s)	82, 228, 4942, 5842
BtgZI	GCGATG	1 cut(s)	1454
BtrI	CACGTC	1 cut(s)	1615
BtsI	GCAGTG	1 cut(s)	5403
BtsIMutI	CAGTG	5 cut(s)	3240, 3394, 4608, 5321, 5403
BveI	ACCTGC	3 cut(s)	990, 1345, 1992
CciI	TCATGA	2 cut(s)	1587, 3091
CfoI	GCGC	9 cut(s)	46, 113, 235, 384, 588, 1288, 1818, 3168, 4932
Cfr10I	RCCGGY	2 cut(s)	107, 5231
ClaI	ATCGAT	1 cut(s)	147
CseI	GACGC	3 cut(s)	1977, 3358, 5681
Csp6I	GTAC	9 cut(s)	1054, 2099, 2386, 2815, 3382, 3398, 4555, 5089, 5734
CspAI	ACCGGT	1 cut(s)	5231
CviQI	GTAC	9 cut(s)	1054, 2099, 2386, 2815, 3382, 3398, 4555, 5089, 5734
DinI	GGCGCC	1 cut(s)	112
DraI	TTTAAA	1 cut(s)	1551
DriI	GACNNNNNGTC	1 cut(s)	3100
EaeI	YGGCCR	2 cut(s)	73, 2143
Eam1104I	CTCTTC	4 cut(s)	2952, 4465, 5580, 5672
Eam1105I	GACNNNNNGTC	1 cut(s)	3100
EarI	CTCTTC	4 cut(s)	2952, 4465, 5580, 5672
EciI	GGCGGA	2 cut(s)	5268, 5494
Ecl136II	GAGCTC	3 cut(s)	69, 1701, 2432
Eco130I	CCWWGG	2 cut(s)	228, 4942
Eco24I	GRGCYC	4 cut(s)	71, 1703, 2347, 2434
Eco32I	GATATC	2 cut(s)	412, 762
Eco47I	GGWCC	8 cut(s)	244, 1410, 1440, 1571, 2263, 3609, 4774, 5229
Eco47III	AGCGCT	1 cut(s)	383
Eco53kI	GAGCTC	3 cut(s)	69, 1701, 2432
Eco57I	CTGAAG	6 cut(s)	257, 1199, 3446, 4856, 5467, 5473
Eco72I	CACGTG	3 cut(s)	3737, 3746, 4373
Eco81I	CCTNAGG	1 cut(s)	5551
Eco88I	CYCGRG	3 cut(s)	128, 4347, 5023
Eco91I	GGTNACC	1 cut(s)	1332
EcoICRI	GAGCTC	3 cut(s)	69, 1701, 2432
EcoNI	CCTNNNNNAGG	1 cut(s)	4106
EcoO65I	GGTNACC	1 cut(s)	1332
EcoRI	GAATTC	1 cut(s)	204
EcoRV	GATATC	2 cut(s)	412, 762
EcoT14I	CCWWGG	2 cut(s)	228, 4942
EcoT22I	ATGCAT	2 cut(s)	2323, 5313
EcoT38I	GRGCYC	4 cut(s)	71, 1703, 2347, 2434
EgeI	GGCGCC	1 cut(s)	112
EheI	GGCGCC	1 cut(s)	112
ErhI	CCWWGG	2 cut(s)	228, 4942
Esp3I	CGTCTC	3 cut(s)	202, 3386, 4467
FalI	AAGNNNNNCTT	8 cut(s)	1155, 1187, 1909, 1941, 2877, 2909, 3544, 3576
FaqI	GGGAC	6 cut(s)	101, 629, 1040, 3500, 5478, 5720
FauI	CCCGC	3 cut(s)	30, 126, 3584
FauNDI	CATATG	4 cut(s)	1125, 2381, 2824, 5083
FbaI	TGATCA	1 cut(s)	1876
FblI	GTMKAC	7 cut(s)	224, 722, 889, 2841, 3521, 4653, 5388
FriOI	GRGCYC	4 cut(s)	71, 1703, 2347, 2434
FspI	TGCGCA	3 cut(s)	587, 1287, 1817
GlaI	GCGC	9 cut(s)	45, 112, 234, 383, 587, 1287, 1817, 3167, 4931
GsuI	CTGGAG	1 cut(s)	184
HaeII	RGCGCY	3 cut(s)	114, 385, 3169
HaeIII	GGCC	9 cut(s)	41, 75, 81, 1360, 2145, 2162, 2713, 3190, 5651
HapII	CCGG	9 cut(s)	72, 108, 765, 1413, 2142, 3821, 5232, 5643, 5871
HgaI	GACGC	3 cut(s)	1977, 3358, 5681
HhaI	GCGC	9 cut(s)	46, 113, 235, 384, 588, 1288, 1818, 3168, 4932
Hin1I	GRCGYC	4 cut(s)	111, 4476, 5692, 5892
Hin6I	GCGC	9 cut(s)	44, 111, 233, 382, 586, 1286, 1816, 3166, 4930
HinP1I	GCGC	9 cut(s)	44, 111, 233, 382, 586, 1286, 1816, 3166, 4930
HincII	GTYRAC	3 cut(s)	2214, 5608, 5890
HindII	GTYRAC	3 cut(s)	2214, 5608, 5890
HindIII	AAGCTT	4 cut(s)	2493, 2972, 3938, 4007
HpaII	CCGG	9 cut(s)	72, 108, 765, 1413, 2142, 3821, 5232, 5643, 5871
Hpy99I	CGWCG	3 cut(s)	127, 396, 4481
Hsp92I	GRCGYC	4 cut(s)	111, 4476, 5692, 5892
HspAI	GCGC	9 cut(s)	44, 111, 233, 382, 586, 1286, 1816, 3166, 4930
KasI	GGCGCC	1 cut(s)	110
Kpn2I	TCCGGA	1 cut(s)	764
KroI	GCCGGC	1 cut(s)	107
KroNI	GCCGGC	1 cut(s)	109
Ksp22I	TGATCA	1 cut(s)	1876
LguI	GCTCTTC	2 cut(s)	5580, 5672
MbiI	CCGCTC	1 cut(s)	28
MfeI	CAATTG	7 cut(s)	19, 492, 675, 861, 1017, 2748, 4707
MflI	RGATCY	5 cut(s)	187, 1479, 2736, 2785, 2907
MhlI	GDGCHC	8 cut(s)	71, 363, 1623, 1703, 2347, 2434, 3751, 4194
Mly113I	GGCGCC	1 cut(s)	111
MlyI	GAGTC	5 cut(s)	1202, 2106, 3527, 5038, 5702
MmeI	TCCRAC	5 cut(s)	541, 1945, 2114, 4193, 5829
Mph1103I	ATGCAT	2 cut(s)	2323, 5313
MreI	CGCCGGCG	1 cut(s)	107
MroI	TCCGGA	1 cut(s)	764
MroNI	GCCGGC	1 cut(s)	107
MroXI	GAANNNNTTC	6 cut(s)	1167, 3357, 3864, 4068, 4210, 4452
MslI	CAYNNNNRTG	3 cut(s)	4317, 4634, 4947
MspA1I	CMGCKG	1 cut(s)	1182
MspCI	CTTAAG	2 cut(s)	1838, 2966
MspI	CCGG	9 cut(s)	72, 108, 765, 1413, 2142, 3821, 5232, 5643, 5871
MunI	CAATTG	7 cut(s)	19, 492, 675, 861, 1017, 2748, 4707
Mva1269I	GAATGC	3 cut(s)	781, 1288, 4158
MvnI	CGCG	5 cut(s)	44, 1971, 2916, 3591, 5564
NaeI	GCCGGC	1 cut(s)	109
NarI	GGCGCC	1 cut(s)	111
NcoI	CCATGG	2 cut(s)	228, 4942
NdeI	CATATG	4 cut(s)	1125, 2381, 2824, 5083
NgoMIV	GCCGGC	1 cut(s)	107
NmeAIII	GCCGAG	1 cut(s)	859
NmuCI	GTSAC	9 cut(s)	254, 1332, 1711, 3238, 3732, 4255, 4368, 5455, 5668
NsbI	TGCGCA	3 cut(s)	587, 1287, 1817
NsiI	ATGCAT	2 cut(s)	2323, 5313
NspI	RCATGY	4 cut(s)	787, 2200, 3331, 4303
PaeI	GCATGC	1 cut(s)	4303
PaeR7I	CTCGAG	1 cut(s)	5023
PagI	TCATGA	2 cut(s)	1587, 3091
PaqCI	CACCTGC	2 cut(s)	1345, 1992
PciI	ACATGT	1 cut(s)	783
PciSI	GCTCTTC	2 cut(s)	5580, 5672
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 3742
PctI	GAATGC	3 cut(s)	781, 1288, 4158
PdiI	GCCGGC	1 cut(s)	109
PdmI	GAANNNNTTC	6 cut(s)	1167, 3357, 3864, 4068, 4210, 4452
PflFI	GACNNNGTC	1 cut(s)	1971
PflMI	CCANNNNNTGG	1 cut(s)	1507
PfoI	TCCNGGA	2 cut(s)	1846, 3176
PinAI	ACCGGT	1 cut(s)	5231
PleI	GAGTC	5 cut(s)	1201, 2106, 3526, 5037, 5702
PluTI	GGCGCC	1 cut(s)	114
PmaCI	CACGTG	3 cut(s)	3737, 3746, 4373
PmlI	CACGTG	3 cut(s)	3737, 3746, 4373
PpsI	GAGTC	5 cut(s)	1201, 2106, 3526, 5037, 5702
Ppu21I	YACGTR	4 cut(s)	3737, 3746, 4195, 4373
PscI	ACATGT	1 cut(s)	783
PshAI	GACNNNNGTC	1 cut(s)	5462
Psp124BI	GAGCTC	3 cut(s)	71, 1703, 2434
PspCI	CACGTG	3 cut(s)	3737, 3746, 4373
PspEI	GGTNACC	1 cut(s)	1332
PspXI	VCTCGAGB	1 cut(s)	5023
PstI	CTGCAG	6 cut(s)	169, 1187, 2003, 3259, 3543, 4798
PsuI	RGATCY	5 cut(s)	187, 1479, 2736, 2785, 2907
PsyI	GACNNNGTC	1 cut(s)	1971
PvuII	CAGCTG	1 cut(s)	1182
RsaI	GTAC	9 cut(s)	1055, 2100, 2387, 2816, 3383, 3399, 4556, 5090, 5735
RsaNI	GTAC	9 cut(s)	1054, 2099, 2386, 2815, 3382, 3398, 4555, 5089, 5734
RseI	CAYNNNNRTG	3 cut(s)	4317, 4634, 4947
SacI	GAGCTC	3 cut(s)	71, 1703, 2434
SapI	GCTCTTC	2 cut(s)	5580, 5672
ScaI	AGTACT	1 cut(s)	2100
SchI	GAGTC	5 cut(s)	1202, 2106, 3527, 5038, 5702
SduI	GDGCHC	8 cut(s)	71, 363, 1623, 1703, 2347, 2434, 3751, 4194
SfoI	GGCGCC	1 cut(s)	112
Sfr274I	CTCGAG	1 cut(s)	5023
SgrAI	CRCCGGYG	1 cut(s)	107
SinI	GGWCC	8 cut(s)	244, 1410, 1440, 1571, 2263, 3609, 4774, 5229
SlaI	CTCGAG	1 cut(s)	5023
SmiMI	CAYNNNNRTG	3 cut(s)	4317, 4634, 4947
SpeI	ACTAGT	1 cut(s)	3713
SphI	GCATGC	1 cut(s)	4303
SspDI	GGCGCC	1 cut(s)	110
SspI	AATATT	6 cut(s)	640, 1325, 2175, 3265, 4384, 5586
SstI	GAGCTC	3 cut(s)	71, 1703, 2434
StyI	CCWWGG	2 cut(s)	228, 4942
TaqI	TCGA	6 cut(s)	147, 2206, 2453, 4162, 5024, 5815
TaqII	GACCGA	1 cut(s)	4344
TatI	WGTACW	4 cut(s)	1053, 2098, 2814, 4554
TauI	GCSGC	3 cut(s)	31, 44, 1262
TscAI	CASTG	5 cut(s)	3247, 3394, 4615, 5328, 5410
TseFI	GTSAC	9 cut(s)	254, 1332, 1711, 3238, 3732, 4255, 4368, 5455, 5668
Tsp45I	GTSAC	9 cut(s)	254, 1332, 1711, 3238, 3732, 4255, 4368, 5455, 5668
TspGWI	ACGGA	4 cut(s)	787, 3517, 4683, 5172
TspRI	CASTG	5 cut(s)	3247, 3394, 4615, 5328, 5410
Tth111I	GACNNNGTC	1 cut(s)	1971
Van91I	CCANNNNNTGG	1 cut(s)	1507
Vha464I	CTTAAG	2 cut(s)	1838, 2966
VneI	GTGCAC	1 cut(s)	3747
VpaK11BI	GGWCC	8 cut(s)	244, 1410, 1440, 1571, 2263, 3609, 4774, 5229
XagI	CCTNNNNNAGG	1 cut(s)	4106
XbaI	TCTAGA	2 cut(s)	649, 1664
XceI	RCATGY	4 cut(s)	787, 2200, 3331, 4303
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2257
XhoI	CTCGAG	1 cut(s)	5023
XmiI	GTMKAC	7 cut(s)	224, 722, 889, 2841, 3521, 4653, 5388
XmnI	GAANNNNTTC	6 cut(s)	1167, 3357, 3864, 4068, 4210, 4452
ZraI	GACGTC	2 cut(s)	4477, 5893
ZrmI	AGTACT	1 cut(s)	2100
Zsp2I	ATGCAT	2 cut(s)	2323, 5313

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.