Rroxscaffold_157G00438350

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000157
OG0002438
Physical Location & Seq
Forward (+)
34775 .. 61576
26802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_157G00438350.1

Sequence Viewer

Length: 6042 bp
ATGACTAGGGTTTACGACAATTGGGAGCGGCTGGTGCGAGCCACTCTCAAGAGAGAGCAGCTCAGAACTTCCGGCCAAGGCCATGAGAGGGCTCCGAGTGGAATCGCCGGCGCGGTTCCGTCGTCGCTGGGCAAGGTCTCGAATATCGATTTGATCTTGCAGGCTGCCGATGAAATTCTAGCCGAGGATGCAAACATCTCCAGAATTCTGTGTGAGCAGGCGTATAGTATGGCGCAAAATCTTGACCCTACTAGTGATGGTAGAGGAGTTCTTCAGTTTAAGACTGGTTTGATGTCTGTAATTAAGCAAAAACTTGCGAAAAGGGATGGGGGTCAGATAGATCGCAATCGTGATATTGAGCACCTCTGGGAATTTTACCAGCGCTACAAGCGACGACATCACCTGGAGGATATCCAGAGGGAGGAGGAGAGACTGCGTGAATCTGGCACTTTTAGTGCTAACTTTGGAGACTATAAAGAGATGAAAAAGACAATTGCTATCCTGAGGGCATTGATTGAGGTGATGGAGTTGCTCAGCAAAGATGCAGATCCTCATGGAGTTGGAAGGCAAATTACAGAGGAGATTAGAGGTGCAATGTCAGCAATTAGATATACTGAACAATTCCCTAGGCTGCCAGCTAAAGTTGAGATATCTGGACAACGGGATGCGGACATGTTTGATCTTCTGGAATTTGTGTTCGGTTTTCAGAAAGACAATGTAAGGAACCAGAGAGAGAATGTTGTCCTTACAATTGCAAATGCACAATCTCGTCTTGGTATACCTGCTCAGGCTGATCCAAAAATAGACGAGAAAGCAATCAATGAAGTATTTCTGAAAGTCCTGGATAATTATAGCAAATGGTGCAAATACCTGCGGATCCGGCTTGTATGGAATAGTTTACAAGCAATTAATCGGGACAGAAAACTTTTTCTGGTGTCATTGTACTTTCTGATATGGGGTGAGGCTGCAAATGTCCGCTTTCTTCCTGAATGCATTTGCTACATATTTCACCATATGGCGAAAGAGTTGGATGCAATTTTAGATCATGGAGAAGCTATTCCTGCTGCTAGCTGCACAATAGGAAATGATTCTGTGTCTTTCCTTGATCAGATTGTTTATCCTATATATGAAATATTGGCAGCGGAAGCTGCTAGAAACAATAACGGGAAAGCTGCACATTCAGCATGGCGGAATTATGACGACTTCAATGAATACTTTTGGTCACCTGCTTGTTTTGACTTGAGCTGGCCTATGAAGAGGGATTCCACATTTTTGTTGAAGCCTAAAAAGTGGAAAAGGACAGGAAAGAGCACTTTCGTTGAGCATCGAACGTTCATTTTGTGCTTCTGCATATCCTACCGAAGTACCGAGTTACTTTGGATTAAACTCGATGTTGACATATGTTGTGTTTTCTCTTCGAAGGGTTTGGCAATTATAGCCTTTAATGATGGGAAGATCAATTCGGATACAGTGAAGAGTGTACTCAGCATTGGGCCGGCATTCGCTATCATGAACTTCGTAGAAAGTAGTTTGGATGTGTTGCTCATGTTTGGGGCTTATACCACAGCCAGAGGCATGGCTATCTCAAGACTTGTAATTAGATTTTTCTGGTTTGGCTTGAGTTCTGTCGCTGTGACATATATTTATGTGAAGGTTCTGCAGGAAAGAAATGATAAATCAAATGATTCATTCTACTTTCGGATATACATTCTTGTACTGGGTGTTTATGCAGCTTTACGCATAGTCCTTGCGCTATTACTCAAATTTCCGGCATGTCATAAGCTTTCTGAAATGTCGGATCAATCATTTTTCCAATTTTTCAAGTGGATATATGAGGAGCGTTATTTTGTTGGACGTGGTCTTTATGAGAGGATGAGCGATTATCTCAGATCTGTGCTCTTTTGGGTGATTAAGCCTCTTGTTGAACCAACGAGAATTATTGTGAACCTTCCAGCTGTGCAATATGCATGGCATGATTTGGTCTCAAAGAACAACAAAAATGTGTTAACTATAGCCAGCCTATGGGCTCCTGTTGTGGCTATCTATCTCATGGACATTCACATATGGTACACTCTTTTGTCGGCCATAATTGGCGGTGTAATGGGTGCAAGGTCTCGGTTAGGCGAGATACGCTCAATTGAAATGGTGCATAAACGTTTTGAGAGTTTTCCGGAAGCTTTTGTGAAGAATCTAGTTTCGCAATCACAGAAACGAAGGTTTCCGTCTAACAGTGAACCATCTCAGGATTCTCAAGTCTTAAACAAGACAAATGCAACAATATTTTCTCCATTTTGGAATGAGATAATAAAAAGCTTACGAGAGGAGGATTTTATCAGTAATAGGGAGAAGGATTTGCTTTCTATTCCTAGCAACACTGGAAGTCTAAGATTAGTGCAGTGGCCTCTGTTTCTTCTAAGTAGTAAGATCTTGCTTGCAATTGATTTGGCCTTGGACTGCAAAGATACGCAGGCAGATCTTTGGAGCAGAGTATGCAGGGATGAGTACATGGCATATGCTGTTCAGGAATGCTATTTAAGTATTGAAAAAATATTGTATTCTCTGGTTGAGGGTGAAGGGAGACTTTGGGTGGAGAGGATCTATCGAGAGATCAACAACAGTATTATGGAGGGTTCGCTTGTTGTAACTTTAAATCTTGCAAAGCTTCCAGCAGTGCTACGAAAATTTACAGCTTTAACTGGACTGCTGATACGTCCAGAAACCGATGTGCTTGCAAAAGGTGCTGCTAAAGCTGTATTTGATGTATATGATGCCGTTACACATGACCTTCTGTCACCTGATTTGAGGGAGCAGCTCGACACATGGAGCCTTTTAGCAAAAGCAAGAAATGAAGGGCGCTTGTTTTCTAGGATAAAGTGGCCCAGTGACCCAGAAACTAAAGACCTGGTGAAGCGACTATACCTTCTTCTTACTGTTAAGGATTCTGCAGCAAATATTCCGAAGAATCTTGAAGCAAGAAGAAGATTAGAATTTTTCACAAATTCGTTATTTATGGACATCCCTTCAGCAAAGCCAGTTAGTGAAATGGTACCTTTCAGTGTATTCACCCCATACTACAGTGAGACTGTACTTTATAGTTCCTCCGAATTGCGGGTTGAAAATGAGGATGGAATTTCTGTTCTTTTTTATCTTCAGAAAATTTTTCCAGATGAGTGGGACAATTTCTTGGAACGAATTGGTCGGGACCAGTCCACTGGGGATGCTGAGCTTCAGGAAAACTCAAGTGATGCTTTGGAGCTTCGGTTTTGGGTATCTTATCGAGGACAGACTTTGGCCAGGACTGTGCGGGGTATGATGTATTATAGAAAGGCTGTAATGTTACAGAGTTATCTTGAAAGAAGGTCATTGGGAGTGGATGATTATTCTCAAGTTGATGCATTCTCAAGTCAAGGCTTTGAGTCATCACTTGAATCACGAGCCCAGGCAGATTTGAAGTTTACTTATGTTGTATCCTGCCAAATCTATGGGCAACAGAAGCAGAGAAGGGCACCAGAGGCTGCTGATATATCGCTCTTGTTGCAGAGGAATGAGGCACTGCGTGTTGCATATATACATGTGGAGGAAACTGCTACAGCTGAAGGAAAGATCTTGAAAGAATTTTACTCAAAGCTTGTAAAAGCTGATATACGTGGAACGGATCAGGAAATATATTCCATTAAACTTCCTGGTGATCCAAAGCTTGGTGAAGGGAAGCCTGAAAATCAAAACCATGCTATAGTCTTCACTCGCGGGGAAGCTGTTCAAACTATTGACATGAACCAGGACAATTACCTTGAGGAGGCAATGAAAATGAGAAATCTTCTCGAGGAGTTTCACAAAAATCATGGTCTTCGCCCCCCATCTATTCTTGGTGTGAGAGAGCATGTATTTACTGGAAGTGTCTCATCTTTAGCATGGTTCATGTCCAATCAAGAGACTAGCTTTGTAACTTTAGCTCAACGTGTTTTAGCATTCCCTCTCAAAGTTCGTATGCATTATGGGCATCCTGATGTGTTTGACCGCATCTTTCACATTACTCGAGGTGGAATTAGTAAAGCTTCACGTGTTATTAACATCAGTGAAGATATTTTTGCAGGGTTCAACTCCACATTAAGGCAGGGTAACATTACCCACCACGAATACATTCAGGTTGGGAAAGGAAGGGATGTTGGCCTGAACCAGATTGCATTGTTTGAAGGGAAAGTTGCAGGTGGAAATGGGGAGCAAGTCCTTAGTAGGGATGTCTACAGGCTTGGGCAACTTCTTGATTTTTTCAGAATGTTCTCTTTCTTCTACACAACAGTTGGTTACTATGTGTGTACAATGATGACCGTCCTCATTGTATACATCTTCCTGTATGGAAGGGCGTACCTGGCTTTCTCTGGACTTGATCGTGCAATTGCGAAGGAAGCAAAAATATCGGGTAACACAGCATTAGATGCTGTTCTTAATGCACAGTTCTTGGTGCAGATTGGAATTTTCACAGCAGTCCCAATGATAATGGGTTTTATACTTGAATTGGGATTACTAAAGGCGGTTTTCAGTTTTATCACTATGCAGCTGCAGTTGTGTTCTGTGTTCTTCACGTTCTCTTTGGGAACTAGAACTCATTATTTTGGACGTACCATCCTCCATGGAGGTGCAAAATACCGAGCAACTGGTAGGGGGTTTGTTGTCCGACACATAAAGTTTGCTGATAATTATAGGCTCTACTCTAGAAGCCATTTCGTCAAAGCGCTTGAAGTGGCTCTTCTCCTCATAGTTTACATTGCATATGGGTATGCGGATGGTGGTGCTGTTTCTTACATTCTGCTAACTCTTAGCAGTTGGTTCCTTGTCATCTCCTGGCTGTTTGCTCCCTACATCTTTAATCCTTCTGGTTTTGAGTGGCAAAAGACAGTGGAAGATTTTGATGATTGGACAAGTTGGCTTCTTTACAAAGGTGGAGTAGGAGTTAAGGGAGAAAATAGCTGGGAATCTTGGTGGGATGAGGAGCAGATGCATATTCAAACATTGAGAGGTCGTATCTTGGAAACAATTTTGAGCTTGAGATTCTTCTTATTTCAATATGGCATTGTTTACAAACTCCATCTCACTGGGAAGGACACATCACTTGCGATATACGGCTTCTCTTGGGTTGTGTTGATAACTATTGTCATGATATTCAAGGTGTTCACCTTGAGTCAAAAAAAGTCGGTCAAATTCCAGCTGTTCATGAGATTTACACAGGGTGTCACGTCCATGGGACTGGTTGCAGCTATTGTTCTACTTGTTATATTCACGAGGCTTTCAATAGCAGACCTGTTTGCAAGCATACTTGCAATCATTCCAACCGGATGGGCCATAATATGTCTTGCCATCACGTGGAAGAGCATTGTCAGGAGTCTTGGTTTATGGGACTCGGTGAGGGAGTTTGCCAGAATGTACGATGCTGGTATGGGTATGCTCATTTTTGCTCCTATAGCATTCTTGTCATGGTTCCCGTTCATCTCCACCTTCCAGTCTCGGCTCCTGTTTAACCAAGCATTTAGTCGCGGGTTGGAGATCTCTCTCATCCTTGCTGGAAACAAAGCTAACCTAGCTCTTATGATTGCTGCCAATGCTATTCCTGGTTCCAAACAGATAATTCTAGTTCAGACTAATTGGACATGCATTGATGCCTTGCATGGTAAGACATCTAATGCTTCTACCACTGCGTTGGATAACCTGGAACTATGTCGGCTGCGAGCTCCTCCATCCATGGCTTCGCAGGATAAAGGTCGGCCCTTACCAAAATTTGGTGAGTGGGATGTAAATAACCCAGCATCAGCTGAAGGATTTACTGTCATATTCAACAAGGCCCGGGATGATAAGAAGACCAACGCGTCTCATCCTAATATTGTGACCCCACGAAAAGGCGATAGCAGCGTTCGAAGAGAAGAAGTGGTTTTGCTGTGGTTGAATCCACACACTGCTTTGGAGCCTGGTGATAATGCAAGAGGAGCTTACAAGGACACTGCTTTCTTGTGCCTGCGACACATGTGGAGCAGTCTATCTCATATATGA

Protein Analysis

2013

Amino Acids

229.5

Weight (kDa)

8.72

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 308 - 416 2.3e-40 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 801 - 978 1.2e-45 Callose synthase 1 domain
Glucan_synthase PF02364 985 - 1762 1.2e-229 1,3-beta-glucan synthase component
AvrRpt-cleavage PF05627 1918 - 1951 1.5e-17 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1234, 4196
AasI GACNNNNNNGTC 1 cut(s) 5860
Acc36I ACCTGC 4 cut(s) 790, 879, 1234, 4196
Acc65I GGTACC 1 cut(s) 3046
AccB1I GGYRCC 2 cut(s) 3046, 3505
AccB7I CCANNNNNTGG 4 cut(s) 2022, 3698, 5361, 5774
AccBSI CCGCTC 1 cut(s) 28
AccI GTMKAC 3 cut(s) 778, 4242, 4341
AccII CGCG 4 cut(s) 113, 3747, 5532, 5861
AccIII TCCGGA 1 cut(s) 2170
AclI AACGTT 2 cut(s) 1331, 2155
AclWI GGATC 8 cut(s) 542, 788, 871, 884, 1806, 2603, 3663, 3683
AcoI YGGCCR 3 cut(s) 73, 2082, 3291
AcuI CTGAAG 6 cut(s) 257, 3006, 3134, 3212, 3615, 5829
AcvI CACGTG 2 cut(s) 4061, 5361
AdeI CACNNNGTG 2 cut(s) 3557, 5228
AfeI AGCGCT 2 cut(s) 383, 4734
AflIII ACRYGT 6 cut(s) 672, 3571, 3958, 4060, 5859, 6015
AhdI GACNNNNNGTC 1 cut(s) 2788
AhlI ACTAGT 1 cut(s) 251
AjiI CACGTC 2 cut(s) 1856, 5235
AloI GAACNNNNNNTCC 4 cut(s) 680, 712, 3120, 3152
Alw21I GWGCWC 4 cut(s) 363, 1313, 1899, 5728
AlwI GGATC 8 cut(s) 542, 788, 871, 884, 1806, 2603, 3663, 3683
AlwNI CAGNNNCTG 1 cut(s) 3515
Ama87I CYCGRG 3 cut(s) 3821, 4035, 5838
Aor13HI TCCGGA 1 cut(s) 2170
Aor51HI AGCGCT 2 cut(s) 383, 4734
AseI ATTAAT 1 cut(s) 909
Asp700I GAANNNNTTC 5 cut(s) 828, 1056, 1087, 3756, 4140
Asp718I GGTACC 1 cut(s) 3046
AspA2I CCTAGG 1 cut(s) 626
AspLEI GCGC 6 cut(s) 113, 235, 384, 1753, 2856, 4735
AspS9I GGNCC 6 cut(s) 1493, 2877, 3202, 5337, 5760, 5836
AsuC2I CCSGG 2 cut(s) 5839, 5840
AsuII TTCGAA 2 cut(s) 1418, 5908
AsuNHI GCTAGC 1 cut(s) 1067
AvaI CYCGRG 3 cut(s) 3821, 4035, 5838
AvaII GGWCC 1 cut(s) 3202
AvrII CCTAGG 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 3508
BaeI ACNNNNGTAYC 2 cut(s) 1348, 1381
BalI TGGCCA 1 cut(s) 3293
BamHI GGATCC 1 cut(s) 876
BanI GGYRCC 2 cut(s) 3046, 3505
BanII GRGCYC 4 cut(s) 94, 2029, 3439, 5728
BarI GAAGNNNNNNTAC 4 cut(s) 556, 588, 2900, 2932
BauI CACGAG 2 cut(s) 3432, 5278
BbrPI CACGTG 2 cut(s) 4061, 5361
BbsI GAAGAC 3 cut(s) 3730, 3839, 5858
Bbv12I GWGCWC 4 cut(s) 363, 1313, 1899, 5728
BceAI ACGGC 2 cut(s) 2756, 5137
BcgI CGANNNNNNTGC 4 cut(s) 2711, 2745, 4398, 4432
BciVI GTATCC 2 cut(s) 1459, 3478
BclI TGATCA 1 cut(s) 1105
BcnI CCSGG 2 cut(s) 5839, 5840
BcuI ACTAGT 1 cut(s) 251
BfmI CTRYAG 9 cut(s) 1658, 2010, 2943, 3073, 3588, 3732, 4243, 4559, 5457
BfoI RGCGCY 3 cut(s) 385, 2857, 4736
BfuAI ACCTGC 4 cut(s) 790, 879, 1234, 4196
BfuI GTATCC 2 cut(s) 1459, 3478
BglII AGATCT 5 cut(s) 1889, 2424, 2473, 3603, 5541
BlnI CCTAGG 1 cut(s) 626
BlpI GCTNAGC 2 cut(s) 533, 3222
Bme18I GGWCC 1 cut(s) 3202
BmeRI GACNNNNNGTC 1 cut(s) 2788
BmeT110I CYCGRG 3 cut(s) 3821, 4035, 5838
BmgBI CACGTC 2 cut(s) 1856, 5235
BmgT120I GGNCC 6 cut(s) 1493, 2877, 3202, 5337, 5760, 5836
BmrI ACTGGG 4 cut(s) 1727, 2874, 3222, 5103
BmtI GCTAGC 1 cut(s) 1071
BmuI ACTGGG 4 cut(s) 1727, 2874, 3222, 5103
BpiI GAAGAC 3 cut(s) 3730, 3839, 5858
BplI GAGNNNNNCTC 6 cut(s) 30, 62, 45, 77, 2117, 2149
BpmI CTGGAG 2 cut(s) 184, 425
Bpu10I CCTNAGC 1 cut(s) 786
Bpu1102I GCTNAGC 2 cut(s) 533, 3222
Bpu14I TTCGAA 2 cut(s) 1418, 5908
BpuMI CCSGG 2 cut(s) 5839, 5840
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 3 cut(s) 3647, 4061, 5361
BsaBI GATNNNNATC 2 cut(s) 345, 546
BsaI GGTCTC 3 cut(s) 142, 1987, 2118
BsaJI CCNNGG 9 cut(s) 76, 183, 626, 2448, 3438, 4630, 5238, 5736, 5838
BsaWI WCCGGW 2 cut(s) 2170, 5330
BsaXI ACNNNNNCTCC 6 cut(s) 3245, 3275, 4935, 4965, 5371, 5401
Bse118I RCCGGY 2 cut(s) 107, 1495
Bse21I CCTNAGG 1 cut(s) 503
Bse3DI GCAATG 3 cut(s) 600, 3807, 4764
Bse8I GATNNNNATC 2 cut(s) 345, 546
BseAI TCCGGA 1 cut(s) 2170
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 9 cut(s) 76, 183, 626, 2448, 3438, 4630, 5238, 5736, 5838
BseJI GATNNNNATC 2 cut(s) 345, 546
BseMI GCAATG 3 cut(s) 600, 3807, 4764
BseMII CTCAG 8 cut(s) 76, 494, 547, 800, 1498, 1900, 2255, 3213
BseSI GKGCMC 1 cut(s) 3508
BseYI CCCAGC 3 cut(s) 127, 4968, 5797
BsgI GTGCAG 4 cut(s) 1057, 1158, 2414, 4484
Bsh1236I CGCG 4 cut(s) 113, 3747, 5532, 5861
BshNI GGYRCC 2 cut(s) 3046, 3505
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 4 cut(s) 363, 1313, 1899, 5728
BsiHKCI CYCGRG 3 cut(s) 3821, 4035, 5838
BsiSI CCGG 8 cut(s) 72, 108, 880, 1496, 1769, 2171, 5331, 5839
BslFI GGGAC 6 cut(s) 929, 3188, 3215, 4472, 5256, 5408
BsmBI CGTCTC 1 cut(s) 5868
BsmFI GGGAC 6 cut(s) 929, 3188, 3215, 4472, 5256, 5408
BsmI GAATGC 6 cut(s) 995, 1499, 2531, 3395, 3968, 5462
Bso31I GGTCTC 3 cut(s) 142, 1987, 2118
BsoBI CYCGRG 3 cut(s) 3821, 4035, 5838
Bsp119I TTCGAA 2 cut(s) 1418, 5908
Bsp1286I GDGCHC 8 cut(s) 94, 363, 1313, 1899, 2029, 3439, 3508, 5728
Bsp13I TCCGGA 1 cut(s) 2170
Bsp1407I TGTACA 1 cut(s) 4316
Bsp1720I GCTNAGC 2 cut(s) 533, 3222
Bsp19I CCATGG 3 cut(s) 4630, 5238, 5736
BspCNI CTCAG 8 cut(s) 75, 495, 546, 799, 1497, 1899, 2254, 3214
BspDI ATCGAT 1 cut(s) 147
BspEI TCCGGA 1 cut(s) 2170
BspFNI CGCG 4 cut(s) 113, 3747, 5532, 5861
BspHI TCATGA 3 cut(s) 1509, 5154, 5211
BspMAI CTGCAG 3 cut(s) 1662, 2947, 4563
BspMI ACCTGC 4 cut(s) 790, 879, 1234, 4196
BspOI GCTAGC 1 cut(s) 1071
BspPI GGATC 8 cut(s) 542, 788, 871, 884, 1806, 2603, 3663, 3683
BspQI GCTCTTC 2 cut(s) 4752, 5360
BspT104I TTCGAA 2 cut(s) 1418, 5908
BspT107I GGYRCC 2 cut(s) 3046, 3505
BspTNI GGTCTC 3 cut(s) 142, 1987, 2118
BsrBI CCGCTC 1 cut(s) 28
BsrDI GCAATG 3 cut(s) 600, 3807, 4764
BsrFI RCCGGY 2 cut(s) 107, 1495
BsrGI TGTACA 1 cut(s) 4316
BssAI RCCGGY 2 cut(s) 107, 1495
BssECI CCNNGG 9 cut(s) 76, 183, 626, 2448, 3438, 4630, 5238, 5736, 5838
BssNAI GTATAC 2 cut(s) 779, 4342
BssSI CACGAG 2 cut(s) 3432, 5278
BssT1I CCWWGG 6 cut(s) 76, 626, 2448, 4630, 5238, 5736
Bst1107I GTATAC 2 cut(s) 779, 4342
Bst2BI CACGAG 2 cut(s) 3432, 5278
Bst6I CTCTTC 6 cut(s) 1250, 1420, 1469, 4752, 5360, 5905
BstAPI GCANNNNNTGC 4 cut(s) 861, 2490, 2738, 4436
BstAUI TGTACA 1 cut(s) 4316
BstBAI YACGTR 3 cut(s) 3647, 4061, 5361
BstBI TTCGAA 2 cut(s) 1418, 5908
BstDSI CCRYGG 3 cut(s) 4630, 5238, 5736
BstEII GGTNACC 1 cut(s) 1221
BstENI CCTNNNNNAGG 1 cut(s) 3794
BstFNI CGCG 4 cut(s) 113, 3747, 5532, 5861
BstH2I RGCGCY 3 cut(s) 385, 2857, 4736
BstHHI GCGC 6 cut(s) 113, 235, 384, 1753, 2856, 4735
BstNSI RCATGY 6 cut(s) 676, 1776, 3575, 3884, 5649, 6019
BstPI GGTNACC 1 cut(s) 1221
BstSFI CTRYAG 9 cut(s) 1658, 2010, 2943, 3073, 3588, 3732, 4243, 4559, 5457
BstSLI GKGCMC 1 cut(s) 3508
BstUI CGCG 4 cut(s) 113, 3747, 5532, 5861
BstV2I GAAGAC 3 cut(s) 3730, 3839, 5858
BstX2I RGATCY 8 cut(s) 547, 876, 1889, 2424, 2473, 2595, 3603, 5541
BstXI CCANNNNNNTGG 8 cut(s) 1576, 3171, 3212, 3482, 5093, 5245, 5334, 5695
BstYI RGATCY 8 cut(s) 547, 876, 1889, 2424, 2473, 2595, 3603, 5541
BstZ17I GTATAC 2 cut(s) 779, 4342
Bsu15I ATCGAT 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 503
BsuI GTATCC 2 cut(s) 1459, 3478
BsuTUI ATCGAT 1 cut(s) 147
BtgI CCRYGG 3 cut(s) 4630, 5238, 5736
BtrI CACGTC 2 cut(s) 1856, 5235
BtsI GCAGTG 6 cut(s) 2402, 2676, 3551, 5688, 5946, 5991
BveI ACCTGC 4 cut(s) 790, 879, 1234, 4196
CaiI CAGNNNCTG 1 cut(s) 3515
CciI TCATGA 3 cut(s) 1509, 5154, 5211
CfoI GCGC 6 cut(s) 113, 235, 384, 1753, 2856, 4735
Cfr10I RCCGGY 2 cut(s) 107, 1495
Cfr13I GGNCC 6 cut(s) 1493, 2877, 3202, 5337, 5760, 5836
Cfr9I CCCGGG 1 cut(s) 5838
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 1 cut(s) 5850
CsiI ACCWGGT 1 cut(s) 2901
DraI TTTAAA 1 cut(s) 2649
DraIII CACNNNGTG 2 cut(s) 3557, 5228
DrdI GACNNNNNNGTC 1 cut(s) 5860
DriI GACNNNNNGTC 1 cut(s) 2788
DseDI GACNNNNNNGTC 1 cut(s) 5860
EaeI YGGCCR 3 cut(s) 73, 2082, 3291
Eam1104I CTCTTC 6 cut(s) 1250, 1420, 1469, 4752, 5360, 5905
Eam1105I GACNNNNNGTC 1 cut(s) 2788
EarI CTCTTC 6 cut(s) 1250, 1420, 1469, 4752, 5360, 5905
EciI GGCGGA 1 cut(s) 1204
Ecl136II GAGCTC 1 cut(s) 5726
Eco130I CCWWGG 6 cut(s) 76, 626, 2448, 4630, 5238, 5736
Eco24I GRGCYC 4 cut(s) 94, 2029, 3439, 5728
Eco31I GGTCTC 3 cut(s) 142, 1987, 2118
Eco32I GATATC 2 cut(s) 412, 651
Eco47I GGWCC 1 cut(s) 3202
Eco47III AGCGCT 2 cut(s) 383, 4734
Eco53kI GAGCTC 1 cut(s) 5726
Eco57I CTGAAG 6 cut(s) 257, 3006, 3134, 3212, 3615, 5829
Eco72I CACGTG 2 cut(s) 4061, 5361
Eco81I CCTNAGG 1 cut(s) 503
Eco88I CYCGRG 3 cut(s) 3821, 4035, 5838
Eco91I GGTNACC 1 cut(s) 1221
EcoICRI GAGCTC 1 cut(s) 5726
EcoNI CCTNNNNNAGG 1 cut(s) 3794
EcoO65I GGTNACC 1 cut(s) 1221
EcoRI GAATTC 1 cut(s) 204
EcoRV GATATC 2 cut(s) 412, 651
EcoT14I CCWWGG 6 cut(s) 76, 626, 2448, 4630, 5238, 5736
EcoT22I ATGCAT 6 cut(s) 995, 1969, 3397, 3993, 5001, 5651
EcoT38I GRGCYC 4 cut(s) 94, 2029, 3439, 5728
ErhI CCWWGG 6 cut(s) 76, 626, 2448, 4630, 5238, 5736
Esp3I CGTCTC 1 cut(s) 5868
FalI AAGNNNNNCTT 8 cut(s) 2565, 2597, 3232, 3264, 4731, 4763, 5965, 5997
FaqI GGGAC 6 cut(s) 929, 3188, 3215, 4472, 5256, 5408
FauI CCCGC 4 cut(s) 3102, 3297, 3740, 5525
FauNDI CATATG 5 cut(s) 1014, 1400, 2063, 2512, 4771
FbaI TGATCA 1 cut(s) 1105
FblI GTMKAC 3 cut(s) 778, 4242, 4341
FriOI GRGCYC 4 cut(s) 94, 2029, 3439, 5728
GlaI GCGC 6 cut(s) 112, 234, 383, 1752, 2855, 4734
GsaI CCCAGC 3 cut(s) 131, 4972, 5801
GsuI CTGGAG 2 cut(s) 184, 425
HaeII RGCGCY 3 cut(s) 385, 2857, 4736
HapII CCGG 8 cut(s) 72, 108, 880, 1496, 1769, 2171, 5331, 5839
HgaI GACGC 1 cut(s) 5850
HhaI GCGC 6 cut(s) 113, 235, 384, 1753, 2856, 4735
Hin6I GCGC 6 cut(s) 111, 233, 382, 1751, 2854, 4733
HinP1I GCGC 6 cut(s) 111, 233, 382, 1751, 2854, 4733
HincII GTYRAC 2 cut(s) 1396, 2007
HindII GTYRAC 2 cut(s) 1396, 2007
HindIII AAGCTT 7 cut(s) 1781, 2175, 2311, 2660, 3626, 3695, 4053
HpaI GTTAAC 1 cut(s) 2007
HpaII CCGG 8 cut(s) 72, 108, 880, 1496, 1769, 2171, 5331, 5839
Hpy99I CGWCG 3 cut(s) 124, 127, 396
HspAI GCGC 6 cut(s) 111, 233, 382, 1751, 2854, 4733
Kpn2I TCCGGA 1 cut(s) 2170
KpnI GGTACC 1 cut(s) 3050
KroI GCCGGC 2 cut(s) 107, 1495
KroNI GCCGGC 2 cut(s) 109, 1497
Ksp22I TGATCA 1 cut(s) 1105
KspAI GTTAAC 1 cut(s) 2007
LguI GCTCTTC 2 cut(s) 4752, 5360
MabI ACCWGGT 1 cut(s) 2901
MbiI CCGCTC 1 cut(s) 28
MfeI CAATTG 6 cut(s) 19, 492, 750, 2136, 2436, 4395
MflI RGATCY 8 cut(s) 547, 876, 1889, 2424, 2473, 2595, 3603, 5541
MhlI GDGCHC 8 cut(s) 94, 363, 1313, 1899, 2029, 3439, 3508, 5728
MlsI TGGCCA 1 cut(s) 3293
MluI ACGCGT 1 cut(s) 5859
MluNI TGGCCA 1 cut(s) 3293
MlyI GAGTC 4 cut(s) 3425, 5188, 5389, 5390
MmeI TCCRAC 8 cut(s) 541, 1008, 1776, 1831, 4699, 5351, 5517, 5676
Mox20I TGGCCA 1 cut(s) 3293
Mph1103I ATGCAT 6 cut(s) 995, 1969, 3397, 3993, 5001, 5651
MreI CGCCGGCG 1 cut(s) 107
MroI TCCGGA 1 cut(s) 2170
MroNI GCCGGC 2 cut(s) 107, 1495
MroXI GAANNNNTTC 5 cut(s) 828, 1056, 1087, 3756, 4140
MscI TGGCCA 1 cut(s) 3293
MslI CAYNNNNRTG 3 cut(s) 4005, 4635, 5237
Msp20I TGGCCA 1 cut(s) 3293
MspA1I CMGCKG 6 cut(s) 1142, 1955, 3593, 4558, 5206, 5807
MspI CCGG 8 cut(s) 72, 108, 880, 1496, 1769, 2171, 5331, 5839
MunI CAATTG 6 cut(s) 19, 492, 750, 2136, 2436, 4395
Mva1269I GAATGC 6 cut(s) 995, 1499, 2531, 3395, 3968, 5462
MvnI CGCG 4 cut(s) 113, 3747, 5532, 5861
NaeI GCCGGC 2 cut(s) 109, 1497
NciI CCSGG 2 cut(s) 5839, 5840
NcoI CCATGG 3 cut(s) 4630, 5238, 5736
NdeI CATATG 5 cut(s) 1014, 1400, 2063, 2512, 4771
NgoMIV GCCGGC 2 cut(s) 107, 1495
NheI GCTAGC 1 cut(s) 1067
NmeAIII GCCGAG 2 cut(s) 208, 5482
NmuCI GTSAC 6 cut(s) 1221, 1633, 2790, 2882, 5230, 5878
NsiI ATGCAT 6 cut(s) 995, 1969, 3397, 3993, 5001, 5651
NspI RCATGY 6 cut(s) 676, 1776, 3575, 3884, 5649, 6019
NspV TTCGAA 2 cut(s) 1418, 5908
PaeR7I CTCGAG 2 cut(s) 3821, 4035
PagI TCATGA 3 cut(s) 1509, 5154, 5211
PaqCI CACCTGC 2 cut(s) 1234, 4196
PciI ACATGT 3 cut(s) 672, 3571, 6015
PciSI GCTCTTC 2 cut(s) 4752, 5360
PctI GAATGC 6 cut(s) 995, 1499, 2531, 3395, 3968, 5462
PdiI GCCGGC 2 cut(s) 109, 1497
PdmI GAANNNNTTC 5 cut(s) 828, 1056, 1087, 3756, 4140
PflFI GACNNNGTC 1 cut(s) 1857
PflMI CCANNNNNTGG 4 cut(s) 2022, 3698, 5361, 5774
PfoI TCCNGGA 1 cut(s) 840
PleI GAGTC 4 cut(s) 3424, 5187, 5388, 5390
PmaCI CACGTG 2 cut(s) 4061, 5361
PmlI CACGTG 2 cut(s) 4061, 5361
PpsI GAGTC 4 cut(s) 3424, 5187, 5388, 5390
Ppu21I YACGTR 3 cut(s) 3647, 4061, 5361
PscI ACATGT 3 cut(s) 672, 3571, 6015
PshBI ATTAAT 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 5728
Psp1406I AACGTT 2 cut(s) 1331, 2155
PspCI CACGTG 2 cut(s) 4061, 5361
PspEI GGTNACC 1 cut(s) 1221
PspFI CCCAGC 3 cut(s) 127, 4968, 5797
PspPI GGNCC 6 cut(s) 1493, 2877, 3202, 5337, 5760, 5836
PspXI VCTCGAGB 1 cut(s) 4035
PstI CTGCAG 3 cut(s) 1662, 2947, 4563
PstNI CAGNNNCTG 1 cut(s) 3515
PsuI RGATCY 8 cut(s) 547, 876, 1889, 2424, 2473, 2595, 3603, 5541
PsyI GACNNNGTC 1 cut(s) 1857
PvuII CAGCTG 5 cut(s) 1955, 3593, 4558, 5206, 5807
RseI CAYNNNNRTG 3 cut(s) 4005, 4635, 5237
SacI GAGCTC 1 cut(s) 5728
SapI GCTCTTC 2 cut(s) 4752, 5360
Sau96I GGNCC 6 cut(s) 1493, 2877, 3202, 5337, 5760, 5836
SchI GAGTC 4 cut(s) 3425, 5188, 5389, 5390
SduI GDGCHC 8 cut(s) 94, 363, 1313, 1899, 2029, 3439, 3508, 5728
SexAI ACCWGGT 1 cut(s) 2901
SfcI CTRYAG 9 cut(s) 1658, 2010, 2943, 3073, 3588, 3732, 4243, 4559, 5457
Sfr274I CTCGAG 2 cut(s) 3821, 4035
SfuI TTCGAA 2 cut(s) 1418, 5908
SgrAI CRCCGGYG 1 cut(s) 107
SinI GGWCC 1 cut(s) 3202
SlaI CTCGAG 2 cut(s) 3821, 4035
SmaI CCCGGG 1 cut(s) 5840
SmiMI CAYNNNNRTG 3 cut(s) 4005, 4635, 5237
SpeI ACTAGT 1 cut(s) 251
SspI AATATT 5 cut(s) 1134, 2280, 2550, 2953, 5875
SstI GAGCTC 1 cut(s) 5728
StyI CCWWGG 6 cut(s) 76, 626, 2448, 4630, 5238, 5736
TaqII GACCGA 1 cut(s) 5182
TatI WGTACW 6 cut(s) 942, 1480, 1714, 2502, 3085, 4316
TauI GCSGC 1 cut(s) 31
TseFI GTSAC 6 cut(s) 1221, 1633, 2790, 2882, 5230, 5878
Tsp45I GTSAC 6 cut(s) 1221, 1633, 2790, 2882, 5230, 5878
TspGWI ACGGA 3 cut(s) 108, 2211, 3668
TspMI CCCGGG 1 cut(s) 5838
Tth111I GACNNNGTC 1 cut(s) 1857
Van91I CCANNNNNTGG 4 cut(s) 2022, 3698, 5361, 5774
VpaK11BI GGWCC 1 cut(s) 3202
VspI ATTAAT 1 cut(s) 909
XagI CCTNNNNNAGG 1 cut(s) 3794
XbaI TCTAGA 1 cut(s) 4712
XceI RCATGY 6 cut(s) 676, 1776, 3575, 3884, 5649, 6019
XhoI CTCGAG 2 cut(s) 3821, 4035
XmaI CCCGGG 1 cut(s) 5838
XmaJI CCTAGG 1 cut(s) 626
XmiI GTMKAC 3 cut(s) 778, 4242, 4341
XmnI GAANNNNTTC 5 cut(s) 828, 1056, 1087, 3756, 4140
Zsp2I ATGCAT 6 cut(s) 995, 1969, 3397, 3993, 5001, 5651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.