Rh7BG121600

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0002438
Physical Location & Seq
Forward (+)
9053148 .. 9056798
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG121600.1

Sequence Viewer

Length: 930 bp
ATGGATTTGTCGACTTGCTTCTGGAATCTGCTGAGGATTCAGAATTGGGCATCTTGGATGCTTCAAATGAGTGTGCTCTCCGCTGAAGTTGGAAGAAATTGGCAGCAGGTTGCCACCGAATTGCAGTGCAACTGTGACTGTGCGAATGGCTCAGTCACTGGTTTCTGCGCGTCATGCTGGTTACAGGCTTTTGAATTGTTGGGGAGTTGGGACTGGCTGGGCTGTGGAGGATACGAAGGACAAGATTACAAAGCTCTTAGATTAGATCAGTATGAAAGTGGGGGTGTCGTGGCTGAAGCCTGCCAGAGCATTCGTGATATTGGAATGCCTTTATTCAATCACGAGGTGGTGAAGAAGGCACTGGTTTTGGCCATGGAGAAGAATGGCACTATGCTGGATCTGCTTCAGGAGTGCTTCAGCGAAGGACTGATAACAGTCAACCAGATGACAAAAGGCTTTAGCCGGATTAAGGACAAGCTAGATGACTTGGCTCTCGACATTCCAACTGCAAGTGAGAAATTCAGCTTCTATGTGGAGAGGGTTGGGTCTTGCCTACTTTTGGTTCATCTGCTGCGGATTGTTCTCACTGACCATAGGCTGCAAATCTATATAGAGATGAAAAAGACAATTGCTATCTTGAGGGCATTAGTTGAGGAGTTGGAGTCGCTTAGCAAAGATGCAGATCCTTATGGAGTTGGAAGGCAAATTACAGAGTTGCGCAGAATTAAAAGCACTGATGCAACATTATCTGGGGAGCTCACAGCCTACAATATCGTCCCACTGGAAGCACCATTACTAACCAATGCAATTGGAGTATTTCCGGAAGTTAGAGGTGCGATATTGGCAATTAGATATACTGAACAATTCCCTAGGCTGCCAGCTAAGTTTGAGATATCTGGACAATGGGATGCAGATAAAAAGACAATGTAA

Protein Analysis

309

Amino Acids

34.69

Weight (kDa)

5.02

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MA3 PF02847 88 - 181 7e-24 MA3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 719
Acc36I ACCTGC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 11
AccII CGCG 1 cut(s) 170
AccIII TCCGGA 1 cut(s) 820
AciI CCGC 2 cut(s) 81, 574
AclWI GGATC 2 cut(s) 405, 677
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 4 cut(s) 105, 315, 389, 400
AdeI CACNNNGTG 1 cut(s) 346
AfiI CCNNNNNNNGG 2 cut(s) 469, 559
AgsI TTSAA 3 cut(s) 65, 194, 337
AluBI AGCT 5 cut(s) 254, 478, 525, 757, 881
AluI AGCT 5 cut(s) 254, 478, 525, 757, 881
Alw21I GWGCWC 2 cut(s) 78, 759
AlwI GGATC 2 cut(s) 405, 677
AlwNI CAGNNNCTG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 820
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 4 cut(s) 103, 571, 598, 874
ApoI RAATTY 1 cut(s) 518
AspA2I CCTAGG 1 cut(s) 869
AspLEI GCGC 2 cut(s) 170, 720
AsuHPI GGTGA 1 cut(s) 361
AvrII CCTAGG 1 cut(s) 869
BalI TGGCCA 1 cut(s) 371
BanII GRGCYC 1 cut(s) 759
BarI GAAGNNNNNNTAC 4 cut(s) 691, 723, 777, 809
BauI CACGAG 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 78, 759
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 4 cut(s) 115, 558, 585, 861
BciVI GTATCC 1 cut(s) 224
BfaI CTAG 2 cut(s) 479, 870
BfuAI ACCTGC 1 cut(s) 97
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 4 cut(s) 104, 572, 599, 875
BlnI CCTAGG 1 cut(s) 869
BlpI GCTNAGC 1 cut(s) 668
BlsI GCNGC 4 cut(s) 105, 573, 600, 876
BmsI GCATC 5 cut(s) 48, 59, 667, 727, 898
Bpu10I CCTNAGC 1 cut(s) 32
Bpu1102I GCTNAGC 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 658
BsaBI GATNNNNATC 1 cut(s) 681
BsaJI CCNNGG 2 cut(s) 372, 869
BsaWI WCCGGW 1 cut(s) 820
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bsc4I CCNNNNNNNGG 2 cut(s) 469, 559
Bse1I ACTGG 4 cut(s) 163, 218, 366, 786
Bse8I GATNNNNATC 1 cut(s) 681
BseAI TCCGGA 1 cut(s) 820
BseDI CCNNGG 2 cut(s) 372, 869
BseGI GGATG 2 cut(s) 63, 913
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 2 cut(s) 469, 559
BseMII CTCAG 2 cut(s) 23, 165
BseNI ACTGG 4 cut(s) 163, 218, 366, 786
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 4 cut(s) 115, 558, 585, 861
BseYI CCCAGC 1 cut(s) 217
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 1 cut(s) 371
BsiHKAI GWGCWC 2 cut(s) 78, 759
BsiSI CCGG 2 cut(s) 463, 821
BslFI GGGAC 2 cut(s) 224, 761
BslI CCNNNNNNNGG 2 cut(s) 469, 559
BsmFI GGGAC 2 cut(s) 224, 761
BsmI GAATGC 2 cut(s) 309, 330
BsnI GGCC 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 78, 759
Bsp13I TCCGGA 1 cut(s) 820
Bsp143I GATC 3 cut(s) 265, 397, 682
Bsp1720I GCTNAGC 1 cut(s) 668
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 2 cut(s) 81, 574
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 2 cut(s) 24, 164
BspEI TCCGGA 1 cut(s) 820
BspFNI CGCG 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 2 cut(s) 405, 677
BsrI ACTGG 4 cut(s) 163, 218, 366, 786
BssECI CCNNGG 2 cut(s) 372, 869
BssMI GATC 3 cut(s) 265, 397, 682
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 2 cut(s) 372, 869
Bst2BI CACGAG 1 cut(s) 341
Bst4CI ACNGT 3 cut(s) 134, 140, 436
BstC8I GCNNGC 2 cut(s) 301, 879
BstDEI CTNAG 5 cut(s) 32, 151, 257, 668, 882
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 63, 913
BstFNI CGCG 1 cut(s) 170
BstHHI GCGC 2 cut(s) 170, 720
BstKTI GATC 3 cut(s) 268, 400, 685
BstMBI GATC 3 cut(s) 265, 397, 682
BstMWI GCNNNNNNNGC 3 cut(s) 174, 400, 842
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 4 cut(s) 115, 558, 585, 861
BstX2I RGATCY 2 cut(s) 397, 682
BstYI RGATCY 2 cut(s) 397, 682
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 1 cut(s) 371
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 63, 913
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 6 cut(s) 131, 156, 359, 585, 732, 779
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 301, 879
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 2 cut(s) 170, 720
CseI GACGC 1 cut(s) 159
CviAII CATG 2 cut(s) 174, 373
DdeI CTNAG 5 cut(s) 32, 151, 257, 668, 882
DpnI GATC 3 cut(s) 267, 399, 684
DpnII GATC 3 cut(s) 265, 397, 682
DraIII CACNNNGTG 1 cut(s) 346
EaeI YGGCCR 1 cut(s) 369
Ecl136II GAGCTC 1 cut(s) 757
Eco130I CCWWGG 2 cut(s) 372, 869
Eco24I GRGCYC 1 cut(s) 759
Eco32I GATATC 1 cut(s) 894
Eco53kI GAGCTC 1 cut(s) 757
Eco57I CTGAAG 4 cut(s) 105, 315, 389, 400
EcoICRI GAGCTC 1 cut(s) 757
EcoRV GATATC 1 cut(s) 894
EcoT14I CCWWGG 2 cut(s) 372, 869
EcoT38I GRGCYC 1 cut(s) 759
ErhI CCWWGG 2 cut(s) 372, 869
FaeI CATG 2 cut(s) 177, 376
FaqI GGGAC 2 cut(s) 224, 761
FatI CATG 2 cut(s) 173, 372
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 4 cut(s) 104, 572, 599, 875
FokI GGATG 2 cut(s) 70, 920
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 4 cut(s) 104, 572, 599, 875
FspBI CTAG 2 cut(s) 479, 870
FspI TGCGCA 1 cut(s) 719
GlaI GCGC 2 cut(s) 169, 719
GluI GCNGC 4 cut(s) 104, 572, 599, 875
GsaI CCCAGC 1 cut(s) 221
HaeIII GGCC 1 cut(s) 371
HapII CCGG 2 cut(s) 463, 821
HgaI GACGC 1 cut(s) 159
HhaI GCGC 2 cut(s) 170, 720
Hin1II CATG 2 cut(s) 177, 376
Hin6I GCGC 2 cut(s) 168, 718
HinP1I GCGC 2 cut(s) 168, 718
HincII GTYRAC 2 cut(s) 12, 439
HindII GTYRAC 2 cut(s) 12, 439
HinfI GANTC 3 cut(s) 25, 37, 662
HpaII CCGG 2 cut(s) 463, 821
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 12, 439
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 8 cut(s) 22, 314, 341, 407, 494, 637, 821, 897
Hpy8I GTNNAC 2 cut(s) 12, 439
HpyAV CCTTC 4 cut(s) 230, 349, 416, 693
HpyCH4III ACNGT 3 cut(s) 134, 140, 436
HpyCH4V TGCA 8 cut(s) 124, 129, 509, 601, 680, 740, 806, 911
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 400, 842
HpyF3I CTNAG 5 cut(s) 32, 151, 257, 668, 882
Hsp92II CATG 2 cut(s) 177, 376
HspAI GCGC 2 cut(s) 168, 718
Kpn2I TCCGGA 1 cut(s) 820
Kzo9I GATC 3 cut(s) 265, 397, 682
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 4 cut(s) 115, 558, 585, 861
LweI GCATC 5 cut(s) 48, 59, 667, 727, 898
MaeI CTAG 2 cut(s) 479, 870
MaeIII GTNAC 3 cut(s) 134, 154, 180
MalI GATC 3 cut(s) 267, 399, 684
MboI GATC 3 cut(s) 265, 397, 682
MboII GAAGA 3 cut(s) 105, 364, 391
MfeI CAATTG 2 cut(s) 627, 807
MflI RGATCY 2 cut(s) 397, 682
MhlI GDGCHC 2 cut(s) 78, 759
MlsI TGGCCA 1 cut(s) 371
MluNI TGGCCA 1 cut(s) 371
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 4 cut(s) 70, 527, 639, 676
MnlI CCTC 7 cut(s) 27, 221, 337, 531, 633, 646, 824
Mox20I TGGCCA 1 cut(s) 371
MroI TCCGGA 1 cut(s) 820
MscI TGGCCA 1 cut(s) 371
MseI TTAA 2 cut(s) 468, 726
Msp20I TGGCCA 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 2 cut(s) 463, 821
MunI CAATTG 2 cut(s) 627, 807
Mva1269I GAATGC 2 cut(s) 309, 330
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 174, 400, 842
NcoI CCATGG 1 cut(s) 372
NdeII GATC 3 cut(s) 265, 397, 682
NlaIII CATG 2 cut(s) 177, 376
NmuCI GTSAC 2 cut(s) 134, 154
NsbI TGCGCA 1 cut(s) 719
PctI GAATGC 2 cut(s) 309, 330
PfeI GAWTC 2 cut(s) 25, 37
PkrI GCNGC 4 cut(s) 105, 573, 600, 876
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
Psp124BI GAGCTC 1 cut(s) 759
PspFI CCCAGC 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 2 cut(s) 397, 682
SacI GAGCTC 1 cut(s) 759
SalI GTCGAC 1 cut(s) 10
SaqAI TTAA 2 cut(s) 468, 726
SatI GCNGC 4 cut(s) 104, 572, 599, 875
Sau3AI GATC 3 cut(s) 265, 397, 682
SchI GAGTC 1 cut(s) 671
SduI GDGCHC 2 cut(s) 78, 759
SetI ASST 8 cut(s) 111, 256, 348, 480, 527, 759, 835, 883
SfaNI GCATC 5 cut(s) 48, 59, 667, 727, 898
SmlI CTYRAG 1 cut(s) 637
SmoI CTYRAG 1 cut(s) 637
SsiI CCGC 2 cut(s) 81, 574
SspMI CTAG 2 cut(s) 479, 870
SstI GAGCTC 1 cut(s) 759
StyI CCWWGG 2 cut(s) 372, 869
TaaI ACNGT 3 cut(s) 134, 140, 436
TaqI TCGA 2 cut(s) 11, 495
TfiI GAWTC 2 cut(s) 25, 37
Tru1I TTAA 2 cut(s) 468, 726
Tru9I TTAA 2 cut(s) 468, 726
TscAI CASTG 6 cut(s) 131, 163, 366, 592, 739, 786
TseFI GTSAC 2 cut(s) 134, 154
TseI GCWGC 4 cut(s) 103, 571, 598, 874
Tsp45I GTSAC 2 cut(s) 134, 154
TspDTI ATGAA 3 cut(s) 288, 554, 632
TspRI CASTG 6 cut(s) 131, 163, 366, 592, 739, 786
XapI RAATTY 1 cut(s) 518
XmaJI CCTAGG 1 cut(s) 869
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 2 cut(s) 479, 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.