Rroxscaffold_3G00263840

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002438
Physical Location & Seq
Reverse (-)
57653163 .. 57677512
24350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263840.1

Sequence Viewer

Length: 5070 bp
ATGACTAGGGTTTACGACAATTGGGAGCGGCTGGTGCGAGCCACGCTCAAGAGAGAGCAGCTCAGAACTTCCGGCCAAGGCCATGAGAGGGCTCCGAGTGGAATCGCCGGCGCGGTTCCGTCGTCGCTGGGCAAGGTCTCGAATATCGATTTGATCTTGCAGGCTGCCGATGAAATTCTAGCCGAGGATGCAAACATCTCCAGAATTCTGTGTGAGCAGGCGTATAGTATGGCGCAAAATCTTGACCCTACTAGTGATGGTAGAGGAGTTCTTCAGTTTAAGACTGGTTTGATGTCTGTAATTAAGCAAAAACTTGCGAAAAGGGATGGGGGTCAGATAGATCGCAATCGTGATATTGAGCACCTCTGGGAATTTTACCAGCGCTACAAGCGACGACATCACCTGGAGGATATCCAGAGGGAGGAGGAGAGACTGCGTGAATCTGGCACTTTTAGTGCTAACTTTGGAGACTATAAAGAGATGAAAAAGACAATTGCTATCCTGAGGGCATTGATTGAGGTGATGGAGTTGCTCAGCAAAGATGCAGATCCTCATGGAGTTGGAAGGCAAATTACAGAGGAGATTAGAGGTGCAATGTCAGCAATTAGATATACTGAACAATTCCCTAGGCTGCCAGCTAAAGTTGAGATATCTGGACAACGGGATGCGGACATGTTTGATCTTCTGGAATTTGTGTTCGGTTTTCAGAAAGACAATGTAAGGAACCAGAGAGAGAATGTTGTCCTTACAATTGCAAATGCACAATCTCGCCTTGGTATACCTGCTCAGGCTGATCCAATGGCGAAAGAGTTGGATGCAATTTTAGATCATGGAGAAGCTATTCCTGCTGCTAGCTGCACAATAGGAAATGATTCTGTGTCTTTCCTTGATCAGATTGTTTATCCTATATATGAAACATTGGCAGCGGAAGCTGCTAGAAACAATAACGGGAAAGCTGCACATTCAGCATGGCGGAATTATGACGACTTCAATGAATACTTTTGGTTTGTTTGCTTGTCCTTCAATTTATGGATGAATAATTTGATTCCTTCTTTAAGCCATGGTTTGGCAATTATAGCCTTTAATGATGGGAAGATCAATTCGGATACAGTGAAGAGTGTACTCAGCATTGGGCCGGCATTCGCTATCATGAACTTCGTAGAAAGTAGTTTGGATGTGTTGCTCATGTTTGGGGCTTATACCACAGCCAGAGGCATGGCTATCTCAAGACTTGTAATTAGATTTTTCTGGTTTGGCTTGAGTTCTGTCGCTGTGACATATATTTATGTGAAGGTTCTGCAGGAAAGAAATGATAAATCAAATGATTCATTCTACTTTCGGATATACATTCTTGTACTGGGTGTTTATGCAGCTTTACGCCTAGTCCTTGCGCTATTACTCAAATTTCCGGCATGTCATAAGCTTTCTGAAATGTCAGATCAATCATTTTTCCAATTTTTCAAGTGGATATATGAGGAGCGTTATTTTGTTGGACGTGGTCTTTATGAGAGGATGAGCGATTATCTCAGGGTGAGGCTGTACCCCGGAGATGATGGAGTTGTTCTCCAGGCTATTAAGCCTCTTGTTGAACCAACGAGAATTATTGTGGACCTTCCAGCTGTGCAATATGCATGGCATGATTTGGTCTCAAAGAACAACAAAAATGTGTTAACTATAGCCAGCCTATGGGCTCCTGTTGTGGCTATCTATCTCATGGACATTCACATATGGTACACTCTTTTGTCGGCCATAATTGGCGGTGTAATGGGTGCAAGGTCTCGGTTAGGCGAGATACGCTCAATTGAAATGGTGCATAAACGTTTTGAGAGTTTTCCGGAAGCTTTTGTGAAGAATCTAGTTTCGCAATCACAGAAACGAAGGGAGAAGGATTTGCTTTCTATTCCTAGCAACACTGGAAGTCTAAGATTAGTGCAGTGGCCTCTGTTTCTTCTAAGTAGTAAGATCTTGCTTGCAATTGATTTGGCCTTGGACTGCAAAGATACGCAGGCAGATCTTTGGAGCAGAGTATGCAGGGATGAGTACATGGCATATGCTGTTCAGGAATGCTACTTAAGTATTGAAAAAATATTGTATTCTCTGGTTGAGGGTGAAGGGAGACTTTGGGTGGAGAGGATCTATCGAGAGATCAACAACAGTATTATGGAGGGTTCGCTTGTTGTAACTTTAAATCTTGCAAAGCTTCCAGCAGTGCTACGAAAATTTACAGCTTTAACTGGACTGCTGATACGTCCAGAAACCGATGTGCTTGCAAAAGGTGCTGCTAAAGCTGTATTTGATGTATATGATGCCATTACACATGACCTTCTGTCACCTGATTTGAGGGAGCAGCTCGACACATGGAGCCTTTTAGCAAAAGCAAGAAATGAAGGGCGCTTGTTTTCTAGGATAAAGTGGCCCAGTGACCCAGAAACTAAAGACCTGGTGAAGCGACTATACCTTCTTCTTACTGTTAAGGATTCTGCAGCAAATATTCCGAAGAATCTTGAAGCAAGAAGAAGATTAGAATTTTTCACAAATTCGTTATTTATGGACATCCCTTCAGCAAAGCCAGTTAGTGAAATGGTACCTTTCAGTGTATTCACCCCATACTACAGTGAGACTGTACTTTATAGTTCCTCCGAATTGCGGGTTGAAAATGAGGATGGAATTTCTGTTCTTTTTTATCTTCAGAAAATTTTTCCAGATGAGTGGGACAATTTCTTGGAACGAATTGGTCGGGACCAGTCCACTGGGGATGCTGAGCTTCAGGAAAACTCAAGTGATGCTTTGGAGCTTCGGTTTTGGGTATCTTATCGAGGACAGACTTTGGCCAGGACTGTGGATGATTATTCTCAAGTTGATGCATTCTCAAGTCAAGGCTTTGAGTCATCACTTGAATCACGAGCCCAGGCAGATTTGAAGTTTACTTATGTTGTATCCTGCCAAATCTATGGGCAACAGAAGCAGAGAAGGGCACCAGAGGCTGCTGATATATCGCTCTTGTTGCAGAGGAATGAGGCACTGCGTGTTGCATATATACATGTGGAGGAAACTGCTACAGCTGAAGGAAAGATCTTGAAAGAATTTTACTCAAAGCTTGTAAAAGCTGATATACGTGGAACGGATCAGTTAATATCAGCTAACATTCAGCACACTTACTCTGAGACATCTGAGTCAGCTGCTTCTTGGGGTAATCATGGTTCTCATGTACACTCACTAGCAATGTTTCAGAAGCTTTTTTTTAGGTGGGCCAGATTTGCCTCAGAGGGCCCAAAAACACGAGTCTGGAAAGAAATATATTCCATTAAACTTCCTGGTGATCCAAAGCTTGGTGAAGGGAAGCCTGAAAATCAAAACCATGCTATAGTCTTCACTCGCGGGGAAGCTGTTCAAACTATTGACATGAACCAGGACAATTACCTTGAGGAGGCAATGAAAATGAGAAATCTTCTCGAGGAGTTTCACAAAAATCATGGTCTTCGCCCCCCATCTATTCTTGGTGTGAGAGAGCATGTATTTACTGGAAGAGTTCGTATGCATTATGGGCATCCTGATGTGTTTGACCGCATCTTTCACATTACTCGAGGTGGAATTAGTAAAGCTTCACGTGTTATTAACATCAGTGAAGATATTTTTGCAGGGTTCAACTCCACATTAAGGCAGGGTAACATTACCCACCACGAATACATTCAGGTTGGGAAAGGAAGGGATGTTGGCCTGAACCAGATTGCATTGTTTGAAGGGAAAGTTGCAGGTGGAAATGGGGAGCAAGTCCTTAGTAGGGATGTCTACAGGCTTGGGCAACTTCTTGATTTTTTCAGAATGTTCTCTTTCTTCTACACAACAGTTGGTTACTATGTGTGTACAATGGTATTTGCTCATATGACTCCTGAGTATTTATTTTCTTATACACTGGTTTCCACAAAGTTCTATTTTCTGGAGGTTTGTGATAACGATTTGTTCACAATGCAGATGACCGTCCTCATTGTATACATCTTCCTGTATGGAAGGGCGTACCTGGCTTTCTCTGGACTTGATCGTGCAATTGCGAAGGAAGCAAAAATATCGGGTAACACAGCATTAGATGCTGTTCTTAATGCACAGTTCTTGGTGCAGATTGGAATTTTCACAGCAGTCCCAATGATAATGGGTTTTATACTTGAATTGGGATTACTAAAGTACCGAGCAACTGGTAGGGGGTTTGTTGTCCGACACATAAAGTTTGCTGATAATTATAGGCTCTACTCTAGAAGCCATTTCGTCAAAGCGCTTGAAGTGGCTCTTCTCCTCATAGTTTACATTGCATATGGGTATGCGGATGGTGGTGCTGTTTCTTACATTCTGCTAACTCTTAGCAGTTGGTTCCTTGTCATCTCCTGGCTGTTTGCTCCCTACATCTTTAATCCTTCTGGTTTTGAGTGGCAAAAGACAGTGGAAGATTTTGATGATTGGACAAGTTGGCTTCTTTACAAAGGTGGAGTAGGAGTTAAGGGAGAAAATAGCTGGGAATCTTGGTGGGATGAGGAGCAGATGCATATTCAAACATTGAGAGGTCGTATCTTGGAAACAATTTTGAGCTTGAGATTCTTCTTATTTCAATATGGCATTGTTTACAAACTCCATCTCACTGGGAAGGACACATCACTTGCGGTGTTCACCTTGAGTCAAAAAAAGTCGGTCAAATTCCAGCTGTTCATGAGATTTACACAGGGTGTCACGTCCATGGGACTGGTTGCAGCTATTGTTCTACTTGTTATATTCACGAGGCTTTCAATAGCAGACCTGTTTGCAAGCATACTTGCAATCATTCCGACCGGATGGGCCATAATATGTCTTGCCATCACGTGGAAGAGCATTGTCAGGAGTCTTGGTTTATGGGACTCGGTGAGGGAGTTTGCCAGAATGTACGATGCTGGTATGGGTATGCTCATTTTTGCTCCTATAGCATTCTTGTCATGGTTCCCGTTCATCTCCACCTTCCAGTCTCGGCTCCTGTTTAACCAAGCATTTAGTCGCGGGTTGGAGATCTCTCTCATCCTTGCTGGAAACAAAGCTAACGTGGATGTCTGA

Protein Analysis

1689

Amino Acids

192.34

Weight (kDa)

6.73

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 267 - 336 9.8e-16 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 647 - 824 1.1e-45 Callose synthase 1 domain
Glucan_synthase PF02364 831 - 1039 6.4e-37 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1092 - 1177 1.4e-32 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1176 - 1308 6.6e-59 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1388 - 1596 9.9e-31 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3743
AasI GACNNNNNNGTC 1 cut(s) 3172
Acc36I ACCTGC 2 cut(s) 790, 3743
Acc65I GGTACC 1 cut(s) 2584
AccB1I GGYRCC 2 cut(s) 2584, 2974
AccB7I CCANNNNNTGG 4 cut(s) 1066, 1686, 3329, 4845
AccBSI CCGCTC 1 cut(s) 28
AccI GTMKAC 3 cut(s) 778, 3789, 3990
AccII CGCG 3 cut(s) 113, 3378, 5016
AccIII TCCGGA 1 cut(s) 1834
AclI AACGTT 1 cut(s) 1819
AclWI GGATC 5 cut(s) 542, 788, 2141, 3132, 3314
AcoI YGGCCR 3 cut(s) 73, 1746, 2829
AcuI CTGAAG 5 cut(s) 257, 2544, 2672, 2750, 3084
AcvI CACGTG 2 cut(s) 3608, 4845
AdeI CACNNNGTG 2 cut(s) 3026, 4712
AfeI AGCGCT 2 cut(s) 383, 4269
AflII CTTAAG 1 cut(s) 2071
AflIII ACRYGT 3 cut(s) 672, 3040, 3607
AhdI GACNNNNNGTC 1 cut(s) 2326
AhlI ACTAGT 1 cut(s) 251
AjiI CACGTC 2 cut(s) 1496, 4719
AjnI CCWGG 9 cut(s) 402, 1566, 2439, 2831, 2907, 3313, 3408, 4017, 4376
AloI GAACNNNNNNTCC 4 cut(s) 680, 712, 2658, 2690
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 9 cut(s) 142, 424, 462, 1651, 1782, 2110, 2612, 3158, 4989
AlwI GGATC 5 cut(s) 542, 788, 2141, 3132, 3314
AlwNI CAGNNNCTG 1 cut(s) 2984
Ama87I CYCGRG 2 cut(s) 3452, 3582
Aor13HI TCCGGA 1 cut(s) 1834
Aor51HI AGCGCT 2 cut(s) 383, 4269
ApaI GGGCCC 1 cut(s) 3272
Asp700I GAANNNNTTC 4 cut(s) 840, 871, 3387, 3687
Asp718I GGTACC 1 cut(s) 2584
AspA2I CCTAGG 1 cut(s) 626
AspLEI GCGC 6 cut(s) 113, 235, 384, 1393, 2394, 4270
AspS9I GGNCC 8 cut(s) 1133, 1609, 2415, 2740, 3249, 3268, 3269, 4821
AsuC2I CCSGG 1 cut(s) 1545
AsuNHI GCTAGC 1 cut(s) 851
AvaI CYCGRG 2 cut(s) 3452, 3582
AvaII GGWCC 2 cut(s) 1609, 2740
AvrII CCTAGG 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 503
BaeGI GKGCMC 2 cut(s) 2977, 3272
BaeI ACNNNNGTAYC 2 cut(s) 4163, 4196
BalI TGGCCA 1 cut(s) 2831
BanI GGYRCC 2 cut(s) 2584, 2974
BanII GRGCYC 4 cut(s) 94, 1693, 2908, 3272
BarI GAAGNNNNNNTAC 4 cut(s) 556, 588, 2438, 2470
BauI CACGAG 3 cut(s) 2901, 3279, 4762
BbrPI CACGTG 2 cut(s) 3608, 4845
BbsI GAAGAC 2 cut(s) 3361, 3470
Bbv12I GWGCWC 1 cut(s) 363
BcgI CGANNNNNNTGC 4 cut(s) 2249, 2283, 4047, 4081
BciT130I CCWGG 9 cut(s) 404, 1568, 2441, 2833, 2909, 3315, 3410, 4019, 4378
BciVI GTATCC 2 cut(s) 1099, 2947
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 1 cut(s) 1545
BcoDI GTCTC 9 cut(s) 142, 424, 462, 1651, 1782, 2110, 2612, 3158, 4989
BcuI ACTAGT 1 cut(s) 251
BfmI CTRYAG 8 cut(s) 1298, 1674, 2481, 2611, 3057, 3363, 3790, 4941
BfoI RGCGCY 3 cut(s) 385, 2395, 4271
BfrI CTTAAG 1 cut(s) 2071
BfuAI ACCTGC 2 cut(s) 790, 3743
BfuI GTATCC 2 cut(s) 1099, 2947
BglII AGATCT 4 cut(s) 1962, 2011, 3072, 5025
BlnI CCTAGG 1 cut(s) 626
BlpI GCTNAGC 2 cut(s) 533, 2760
Bme18I GGWCC 2 cut(s) 1609, 2740
BmeRI GACNNNNNGTC 1 cut(s) 2326
BmeT110I CYCGRG 2 cut(s) 3452, 3582
BmgBI CACGTC 2 cut(s) 1496, 4719
BmgT120I GGNCC 8 cut(s) 1133, 1609, 2415, 2740, 3249, 3268, 3269, 4821
BmrI ACTGGG 4 cut(s) 1367, 2412, 2760, 4638
BmtI GCTAGC 1 cut(s) 855
BmuI ACTGGG 4 cut(s) 1367, 2412, 2760, 4638
BpiI GAAGAC 2 cut(s) 3361, 3470
BplI GAGNNNNNCTC 8 cut(s) 30, 62, 45, 77, 1548, 1580, 1781, 1813
BpmI CTGGAG 4 cut(s) 184, 425, 1550, 3959
Bpu10I CCTNAGC 1 cut(s) 786
Bpu1102I GCTNAGC 2 cut(s) 533, 2760
BpuEI CTTGAG 9 cut(s) 32, 1210, 1279, 2761, 2838, 2854, 3443, 4600, 4681
BpuMI CCSGG 1 cut(s) 1545
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 3 cut(s) 3116, 3608, 4845
BsaBI GATNNNNATC 2 cut(s) 345, 546
BsaI GGTCTC 3 cut(s) 142, 1651, 1782
BsaJI CCNNGG 9 cut(s) 76, 183, 626, 772, 1060, 1543, 1986, 2907, 4722
BsaWI WCCGGW 2 cut(s) 1834, 4814
BsaXI ACNNNNNCTCC 6 cut(s) 2783, 2813, 4470, 4500, 4855, 4885
Bse118I RCCGGY 2 cut(s) 107, 1135
Bse21I CCTNAGG 1 cut(s) 503
Bse3DI GCAATG 4 cut(s) 600, 3228, 3438, 4299
Bse8I GATNNNNATC 2 cut(s) 345, 546
BseAI TCCGGA 1 cut(s) 1834
BseBI CCWGG 9 cut(s) 404, 1568, 2441, 2833, 2909, 3315, 3410, 4019, 4378
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 9 cut(s) 76, 183, 626, 772, 1060, 1543, 1986, 2907, 4722
BseJI GATNNNNATC 2 cut(s) 345, 546
BseMI GCAATG 4 cut(s) 600, 3228, 3438, 4299
BseRI GAGGAG 9 cut(s) 279, 437, 440, 593, 1490, 3440, 3470, 4277, 4538
BseSI GKGCMC 2 cut(s) 2977, 3272
BseYI CCCAGC 2 cut(s) 127, 4503
BsgI GTGCAG 4 cut(s) 841, 942, 1952, 4133
Bsh1236I CGCG 3 cut(s) 113, 3378, 5016
Bsh1285I CGRYCG 1 cut(s) 4815
BshNI GGYRCC 2 cut(s) 2584, 2974
BshVI ATCGAT 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 4815
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 2 cut(s) 3452, 3582
BsiSI CCGG 7 cut(s) 72, 108, 1136, 1409, 1545, 1835, 4815
BslFI GGGAC 5 cut(s) 2726, 2753, 4121, 4740, 4892
BsmAI GTCTC 9 cut(s) 142, 424, 462, 1651, 1782, 2110, 2612, 3158, 4989
BsmFI GGGAC 5 cut(s) 2726, 2753, 4121, 4740, 4892
BsmI GAATGC 4 cut(s) 1139, 2069, 2864, 4946
Bso31I GGTCTC 3 cut(s) 142, 1651, 1782
BsoBI CYCGRG 2 cut(s) 3452, 3582
Bsp120I GGGCCC 1 cut(s) 3268
Bsp1286I GDGCHC 6 cut(s) 94, 363, 1693, 2908, 2977, 3272
Bsp13I TCCGGA 1 cut(s) 1834
Bsp1407I TGTACA 2 cut(s) 3208, 3863
Bsp1720I GCTNAGC 2 cut(s) 533, 2760
Bsp19I CCATGG 2 cut(s) 1060, 4722
BspDI ATCGAT 1 cut(s) 147
BspEI TCCGGA 1 cut(s) 1834
BspFNI CGCG 3 cut(s) 113, 3378, 5016
BspHI TCATGA 2 cut(s) 1149, 4695
BspMAI CTGCAG 2 cut(s) 1302, 2485
BspMI ACCTGC 2 cut(s) 790, 3743
BspOI GCTAGC 1 cut(s) 855
BspPI GGATC 5 cut(s) 542, 788, 2141, 3132, 3314
BspQI GCTCTTC 2 cut(s) 4287, 4844
BspT107I GGYRCC 2 cut(s) 2584, 2974
BspTI CTTAAG 1 cut(s) 2071
BspTNI GGTCTC 3 cut(s) 142, 1651, 1782
BsrBI CCGCTC 1 cut(s) 28
BsrDI GCAATG 4 cut(s) 600, 3228, 3438, 4299
BsrFI RCCGGY 2 cut(s) 107, 1135
BsrGI TGTACA 2 cut(s) 3208, 3863
BssAI RCCGGY 2 cut(s) 107, 1135
BssECI CCNNGG 9 cut(s) 76, 183, 626, 772, 1060, 1543, 1986, 2907, 4722
BssNAI GTATAC 2 cut(s) 779, 3991
BssSI CACGAG 3 cut(s) 2901, 3279, 4762
BssT1I CCWWGG 6 cut(s) 76, 626, 772, 1060, 1986, 4722
Bst1107I GTATAC 2 cut(s) 779, 3991
Bst2BI CACGAG 3 cut(s) 2901, 3279, 4762
Bst2UI CCWGG 9 cut(s) 404, 1568, 2441, 2833, 2909, 3315, 3410, 4019, 4378
Bst6I CTCTTC 4 cut(s) 1109, 3520, 4287, 4844
BstAFI CTTAAG 1 cut(s) 2071
BstAPI GCANNNNNTGC 3 cut(s) 2028, 2276, 4085
BstAUI TGTACA 2 cut(s) 3208, 3863
BstBAI YACGTR 3 cut(s) 3116, 3608, 4845
BstDSI CCRYGG 2 cut(s) 1060, 4722
BstENI CCTNNNNNAGG 1 cut(s) 3425
BstFNI CGCG 3 cut(s) 113, 3378, 5016
BstH2I RGCGCY 3 cut(s) 385, 2395, 4271
BstHHI GCGC 6 cut(s) 113, 235, 384, 1393, 2394, 4270
BstMAI GTCTC 9 cut(s) 142, 424, 462, 1651, 1782, 2110, 2612, 3158, 4989
BstMCI CGRYCG 1 cut(s) 4815
BstNI CCWGG 9 cut(s) 404, 1568, 2441, 2833, 2909, 3315, 3410, 4019, 4378
BstNSI RCATGY 4 cut(s) 676, 1416, 3044, 3515
BstSFI CTRYAG 8 cut(s) 1298, 1674, 2481, 2611, 3057, 3363, 3790, 4941
BstSLI GKGCMC 2 cut(s) 2977, 3272
BstUI CGCG 3 cut(s) 113, 3378, 5016
BstV2I GAAGAC 2 cut(s) 3361, 3470
BstX2I RGATCY 6 cut(s) 547, 1962, 2011, 2133, 3072, 5025
BstXI CCANNNNNNTGG 7 cut(s) 1216, 2709, 2750, 2839, 2951, 4628, 4729
BstYI RGATCY 6 cut(s) 547, 1962, 2011, 2133, 3072, 5025
BstZ17I GTATAC 2 cut(s) 779, 3991
Bsu15I ATCGAT 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 503
BsuI GTATCC 2 cut(s) 1099, 2947
BsuTUI ATCGAT 1 cut(s) 147
BtgI CCRYGG 2 cut(s) 1060, 4722
BtrI CACGTC 2 cut(s) 1496, 4719
BtsI GCAGTG 3 cut(s) 1940, 2214, 3020
BveI ACCTGC 2 cut(s) 790, 3743
CaiI CAGNNNCTG 1 cut(s) 2984
CciI TCATGA 2 cut(s) 1149, 4695
CfoI GCGC 6 cut(s) 113, 235, 384, 1393, 2394, 4270
Cfr10I RCCGGY 2 cut(s) 107, 1135
Cfr13I GGNCC 8 cut(s) 1133, 1609, 2415, 2740, 3249, 3268, 3269, 4821
ClaI ATCGAT 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 2439
DraI TTTAAA 1 cut(s) 2187
DraIII CACNNNGTG 2 cut(s) 3026, 4712
DrdI GACNNNNNNGTC 1 cut(s) 3172
DriI GACNNNNNGTC 1 cut(s) 2326
DseDI GACNNNNNNGTC 1 cut(s) 3172
EaeI YGGCCR 3 cut(s) 73, 1746, 2829
Eam1104I CTCTTC 4 cut(s) 1109, 3520, 4287, 4844
Eam1105I GACNNNNNGTC 1 cut(s) 2326
EarI CTCTTC 4 cut(s) 1109, 3520, 4287, 4844
EciI GGCGGA 1 cut(s) 988
Eco130I CCWWGG 6 cut(s) 76, 626, 772, 1060, 1986, 4722
Eco24I GRGCYC 4 cut(s) 94, 1693, 2908, 3272
Eco31I GGTCTC 3 cut(s) 142, 1651, 1782
Eco32I GATATC 2 cut(s) 412, 651
Eco47I GGWCC 2 cut(s) 1609, 2740
Eco47III AGCGCT 2 cut(s) 383, 4269
Eco57I CTGAAG 5 cut(s) 257, 2544, 2672, 2750, 3084
Eco72I CACGTG 2 cut(s) 3608, 4845
Eco81I CCTNAGG 1 cut(s) 503
Eco88I CYCGRG 2 cut(s) 3452, 3582
EcoNI CCTNNNNNAGG 1 cut(s) 3425
EcoO109I RGGNCCY 1 cut(s) 3268
EcoRI GAATTC 1 cut(s) 204
EcoRII CCWGG 9 cut(s) 402, 1566, 2439, 2831, 2907, 3313, 3408, 4017, 4376
EcoRV GATATC 2 cut(s) 412, 651
EcoT14I CCWWGG 6 cut(s) 76, 626, 772, 1060, 1986, 4722
EcoT22I ATGCAT 4 cut(s) 1633, 2866, 3540, 4536
EcoT38I GRGCYC 4 cut(s) 94, 1693, 2908, 3272
ErhI CCWWGG 6 cut(s) 76, 626, 772, 1060, 1986, 4722
FalI AAGNNNNNCTT 6 cut(s) 2103, 2135, 2770, 2802, 4266, 4298
FaqI GGGAC 5 cut(s) 2726, 2753, 4121, 4740, 4892
FauI CCCGC 3 cut(s) 2640, 3371, 5009
FauNDI CATATG 4 cut(s) 1727, 2050, 3882, 4306
FbaI TGATCA 1 cut(s) 889
FblI GTMKAC 3 cut(s) 778, 3789, 3990
FriOI GRGCYC 4 cut(s) 94, 1693, 2908, 3272
GlaI GCGC 6 cut(s) 112, 234, 383, 1392, 2393, 4269
GsaI CCCAGC 2 cut(s) 131, 4507
GsuI CTGGAG 4 cut(s) 184, 425, 1550, 3959
HaeII RGCGCY 3 cut(s) 385, 2395, 4271
HapII CCGG 7 cut(s) 72, 108, 1136, 1409, 1545, 1835, 4815
HhaI GCGC 6 cut(s) 113, 235, 384, 1393, 2394, 4270
Hin6I GCGC 6 cut(s) 111, 233, 382, 1391, 2392, 4268
HinP1I GCGC 6 cut(s) 111, 233, 382, 1391, 2392, 4268
HincII GTYRAC 1 cut(s) 1671
HindII GTYRAC 1 cut(s) 1671
HindIII AAGCTT 7 cut(s) 1421, 1839, 2198, 3095, 3233, 3326, 3600
HpaI GTTAAC 1 cut(s) 1671
HpaII CCGG 7 cut(s) 72, 108, 1136, 1409, 1545, 1835, 4815
Hpy99I CGWCG 3 cut(s) 124, 127, 396
HpyCH4IV ACGT 8 cut(s) 1495, 1819, 2248, 3115, 3607, 4718, 4844, 5058
HpySE526I ACGT 8 cut(s) 1495, 1819, 2248, 3115, 3607, 4718, 4844, 5058
HspAI GCGC 6 cut(s) 111, 233, 382, 1391, 2392, 4268
Kpn2I TCCGGA 1 cut(s) 1834
KpnI GGTACC 1 cut(s) 2588
KroI GCCGGC 2 cut(s) 107, 1135
KroNI GCCGGC 2 cut(s) 109, 1137
Ksp22I TGATCA 1 cut(s) 889
KspAI GTTAAC 1 cut(s) 1671
LguI GCTCTTC 2 cut(s) 4287, 4844
MabI ACCWGGT 1 cut(s) 2439
MaeII ACGT 8 cut(s) 1495, 1819, 2248, 3115, 3607, 4718, 4844, 5058
MaeIII GTNAC 8 cut(s) 1273, 2179, 2328, 2420, 3665, 3851, 4070, 4714
MbiI CCGCTC 1 cut(s) 28
MfeI CAATTG 6 cut(s) 19, 492, 750, 1800, 1974, 4044
MflI RGATCY 6 cut(s) 547, 1962, 2011, 2133, 3072, 5025
MhlI GDGCHC 6 cut(s) 94, 363, 1693, 2908, 2977, 3272
MlsI TGGCCA 1 cut(s) 2831
MluNI TGGCCA 1 cut(s) 2831
MlyI GAGTC 7 cut(s) 2894, 3182, 3291, 3880, 4672, 4873, 4874
MmeI TCCRAC 6 cut(s) 541, 792, 1471, 4234, 4835, 5001
Mox20I TGGCCA 1 cut(s) 2831
Mph1103I ATGCAT 4 cut(s) 1633, 2866, 3540, 4536
MreI CGCCGGCG 1 cut(s) 107
MroI TCCGGA 1 cut(s) 1834
MroNI GCCGGC 2 cut(s) 107, 1135
MroXI GAANNNNTTC 4 cut(s) 840, 871, 3387, 3687
MscI TGGCCA 1 cut(s) 2831
MslI CAYNNNNRTG 2 cut(s) 3552, 4721
Msp20I TGGCCA 1 cut(s) 2831
MspA1I CMGCKG 5 cut(s) 926, 1619, 3062, 3179, 4690
MspCI CTTAAG 1 cut(s) 2071
MspI CCGG 7 cut(s) 72, 108, 1136, 1409, 1545, 1835, 4815
MunI CAATTG 6 cut(s) 19, 492, 750, 1800, 1974, 4044
Mva1269I GAATGC 4 cut(s) 1139, 2069, 2864, 4946
MvaI CCWGG 9 cut(s) 404, 1568, 2441, 2833, 2909, 3315, 3410, 4019, 4378
MvnI CGCG 3 cut(s) 113, 3378, 5016
NaeI GCCGGC 2 cut(s) 109, 1137
NciI CCSGG 1 cut(s) 1545
NcoI CCATGG 2 cut(s) 1060, 4722
NdeI CATATG 4 cut(s) 1727, 2050, 3882, 4306
NgoMIV GCCGGC 2 cut(s) 107, 1135
NheI GCTAGC 1 cut(s) 851
NmeAIII GCCGAG 2 cut(s) 208, 4966
NmuCI GTSAC 4 cut(s) 1273, 2328, 2420, 4714
NsiI ATGCAT 4 cut(s) 1633, 2866, 3540, 4536
NspI RCATGY 4 cut(s) 676, 1416, 3044, 3515
PaeR7I CTCGAG 2 cut(s) 3452, 3582
PagI TCATGA 2 cut(s) 1149, 4695
PaqCI CACCTGC 1 cut(s) 3743
PciI ACATGT 2 cut(s) 672, 3040
PciSI GCTCTTC 2 cut(s) 4287, 4844
PctI GAATGC 4 cut(s) 1139, 2069, 2864, 4946
PdiI GCCGGC 2 cut(s) 109, 1137
PdmI GAANNNNTTC 4 cut(s) 840, 871, 3387, 3687
PflFI GACNNNGTC 1 cut(s) 1497
PflMI CCANNNNNTGG 4 cut(s) 1066, 1686, 3329, 4845
PleI GAGTC 7 cut(s) 2893, 3181, 3290, 3880, 4671, 4872, 4874
PmaCI CACGTG 2 cut(s) 3608, 4845
PmlI CACGTG 2 cut(s) 3608, 4845
PpsI GAGTC 7 cut(s) 2893, 3181, 3290, 3880, 4671, 4872, 4874
Ppu21I YACGTR 3 cut(s) 3116, 3608, 4845
PscI ACATGT 2 cut(s) 672, 3040
Psp1406I AACGTT 1 cut(s) 1819
Psp6I CCWGG 9 cut(s) 402, 1566, 2439, 2831, 2907, 3313, 3408, 4017, 4376
PspCI CACGTG 2 cut(s) 3608, 4845
PspFI CCCAGC 2 cut(s) 127, 4503
PspGI CCWGG 9 cut(s) 402, 1566, 2439, 2831, 2907, 3313, 3408, 4017, 4376
PspOMI GGGCCC 1 cut(s) 3268
PspPI GGNCC 8 cut(s) 1133, 1609, 2415, 2740, 3249, 3268, 3269, 4821
PspXI VCTCGAGB 1 cut(s) 3582
PsrI GAACNNNNNNTAC 2 cut(s) 3184, 3216
PstI CTGCAG 2 cut(s) 1302, 2485
PstNI CAGNNNCTG 1 cut(s) 2984
PsuI RGATCY 6 cut(s) 547, 1962, 2011, 2133, 3072, 5025
PsyI GACNNNGTC 1 cut(s) 1497
PvuII CAGCTG 4 cut(s) 1619, 3062, 3179, 4690
RseI CAYNNNNRTG 2 cut(s) 3552, 4721
SapI GCTCTTC 2 cut(s) 4287, 4844
Sau96I GGNCC 8 cut(s) 1133, 1609, 2415, 2740, 3249, 3268, 3269, 4821
SchI GAGTC 7 cut(s) 2894, 3182, 3291, 3880, 4672, 4873, 4874
SduI GDGCHC 6 cut(s) 94, 363, 1693, 2908, 2977, 3272
SexAI ACCWGGT 1 cut(s) 2439
SfcI CTRYAG 8 cut(s) 1298, 1674, 2481, 2611, 3057, 3363, 3790, 4941
Sfr274I CTCGAG 2 cut(s) 3452, 3582
SgrAI CRCCGGYG 1 cut(s) 107
SinI GGWCC 2 cut(s) 1609, 2740
SlaI CTCGAG 2 cut(s) 3452, 3582
SmiMI CAYNNNNRTG 2 cut(s) 3552, 4721
SpeI ACTAGT 1 cut(s) 251
SspI AATATT 2 cut(s) 2088, 2491
StyI CCWWGG 6 cut(s) 76, 626, 772, 1060, 1986, 4722
TaiI ACGT 8 cut(s) 1498, 1822, 2251, 3118, 3610, 4721, 4847, 5061
TaqI TCGA 7 cut(s) 140, 147, 2140, 2352, 2815, 3453, 3583
TaqII GACCGA 1 cut(s) 4666
TatI WGTACW 6 cut(s) 1120, 1354, 2040, 2623, 3208, 3863
TauI GCSGC 1 cut(s) 31
TseFI GTSAC 4 cut(s) 1273, 2328, 2420, 4714
Tsp45I GTSAC 4 cut(s) 1273, 2328, 2420, 4714
TspGWI ACGGA 2 cut(s) 108, 3137
Tth111I GACNNNGTC 1 cut(s) 1497
Van91I CCANNNNNTGG 4 cut(s) 1066, 1686, 3329, 4845
Vha464I CTTAAG 1 cut(s) 2071
VpaK11BI GGWCC 2 cut(s) 1609, 2740
XagI CCTNNNNNAGG 1 cut(s) 3425
XbaI TCTAGA 1 cut(s) 4247
XceI RCATGY 4 cut(s) 676, 1416, 3044, 3515
XhoI CTCGAG 2 cut(s) 3452, 3582
XmaJI CCTAGG 1 cut(s) 626
XmiI GTMKAC 3 cut(s) 778, 3789, 3990
XmnI GAANNNNTTC 4 cut(s) 840, 871, 3387, 3687
Zsp2I ATGCAT 4 cut(s) 1633, 2866, 3540, 4536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.