MD14G1207400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004869
Physical Location & Seq
Reverse (-)
29440073 .. 29441111
1039 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1207400.v1.1.491

Sequence Viewer

Length: 450 bp
ATGCCGGGTGACGTCATAGACAAGCAAAGCGCCAACAGCTCCTCGGTAGTATGCACTTGTAATTGCACGGTACCTTTCTTGGCCAGCGGTATCCCAAATCTGGGCCTTGACGATATTCTCATCAACGTGGATACTCCGAGTGGCGAACTCGACGCCGATCGTCGACTTCGACTCGAGGCTGAACTCGTTGCGCGTGAATCTCGACAAAAGGTTGGATTTTCCGACGCCGGAGTCACCAATCAACACCACCTTGAACAGATAATCATAGTCATCATCTGCCTTGCACGCCATGGCTGCCCTCCTCCCTCACAAATCCAAATTCGACTCGGCGGTTGGGTTTGGTTGGGTTCTGGTTTCCGGGTCGGATCCGAATTAGGTCGCCGGATTTATTCAATTGGAAGAGCGACTCGGACTCGGAGGCATGGAACGAAGGGAGGGAGGATTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.17

Weight (kDa)

9.04

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AatII GACGTC 1 cut(s) 15
Acc65I GGTACC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 163
AccII CGCG 1 cut(s) 193
AciI CCGC 2 cut(s) 87, 330
AclWI GGATC 2 cut(s) 360, 373
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 318
AcyI GRCGYC 3 cut(s) 12, 153, 225
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 2 cut(s) 100, 101
AgsI TTSAA 2 cut(s) 254, 393
AluBI AGCT 1 cut(s) 39
AluI AGCT 1 cut(s) 39
AlwI GGATC 2 cut(s) 360, 373
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 2 cut(s) 81, 103
ApeKI GCWGC 1 cut(s) 294
ApoI RAATTY 1 cut(s) 318
Asp718I GGTACC 1 cut(s) 70
AspLEI GCGC 2 cut(s) 32, 193
AspS9I GGNCC 1 cut(s) 103
AsuC2I CCSGG 2 cut(s) 6, 359
AsuHPI GGTGA 2 cut(s) 20, 226
AvaI CYCGRG 1 cut(s) 173
BalI TGGCCA 1 cut(s) 83
BamHI GGATCC 1 cut(s) 365
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 281
BciVI GTATCC 2 cut(s) 101, 124
BcnI CCSGG 2 cut(s) 6, 359
BfoI RGCGCY 1 cut(s) 33
BfuI GTATCC 2 cut(s) 101, 124
BisI GCNGC 1 cut(s) 295
BlsI GCNGC 1 cut(s) 296
Bme1390I CCNGG 2 cut(s) 6, 359
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 2 cut(s) 72, 367
BmrFI CCNGG 2 cut(s) 6, 359
BpuMI CCSGG 2 cut(s) 6, 359
BsaHI GRCGYC 3 cut(s) 12, 153, 225
BsaJI CCNNGG 2 cut(s) 42, 289
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 101
BseDI CCNNGG 2 cut(s) 42, 289
BseLI CCNNNNNNNGG 2 cut(s) 100, 101
BseRI GAGGAG 2 cut(s) 31, 291
BseXI GCAGC 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 83, 105
BshNI GGYRCC 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 4 cut(s) 5, 228, 358, 382
BslI CCNNNNNNNGG 2 cut(s) 100, 101
BsnI GGCC 2 cut(s) 83, 105
BsoBI CYCGRG 1 cut(s) 173
Bsp143I GATC 2 cut(s) 157, 365
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 2 cut(s) 87, 330
BspANI GGCC 2 cut(s) 83, 105
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 72, 367
BspPI GGATC 2 cut(s) 360, 373
BspQI GCTCTTC 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 42, 289
BssMI GATC 2 cut(s) 157, 365
BssNI GRCGYC 3 cut(s) 12, 153, 225
BssT1I CCWWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 394
BstACI GRCGYC 3 cut(s) 12, 153, 225
BstC8I GCNNGC 2 cut(s) 85, 286
BstDSI CCRYGG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 193
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 2 cut(s) 32, 193
BstKTI GATC 2 cut(s) 160, 368
BstMBI GATC 2 cut(s) 157, 365
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 36, 285, 294
BstSCI CCNGG 2 cut(s) 4, 357
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 281
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BsuI GTATCC 2 cut(s) 101, 124
BsuRI GGCC 2 cut(s) 83, 105
BtgI CCRYGG 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 85, 286
CfoI GCGC 2 cut(s) 32, 193
Cfr13I GGNCC 1 cut(s) 103
CseI GACGC 2 cut(s) 161, 233
Csp6I GTAC 1 cut(s) 71
CviAII CATG 2 cut(s) 290, 422
CviJI RGCY 5 cut(s) 39, 83, 105, 179, 294
CviKI_1 RGCY 5 cut(s) 39, 83, 105, 179, 294
CviQI GTAC 1 cut(s) 71
DpnI GATC 2 cut(s) 159, 367
DpnII GATC 2 cut(s) 157, 365
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 2 cut(s) 293, 425
FaiI YATR 5 cut(s) 17, 52, 266, 291, 423
FatI CATG 2 cut(s) 289, 421
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 1 cut(s) 295
Fsp4HI GCNGC 1 cut(s) 295
GlaI GCGC 2 cut(s) 31, 192
GluI GCNGC 1 cut(s) 295
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 2 cut(s) 83, 105
HapII CCGG 4 cut(s) 5, 228, 358, 382
HgaI GACGC 2 cut(s) 161, 233
HhaI GCGC 2 cut(s) 32, 193
Hin1I GRCGYC 3 cut(s) 12, 153, 225
Hin1II CATG 2 cut(s) 293, 425
Hin6I GCGC 2 cut(s) 30, 191
HinP1I GCGC 2 cut(s) 30, 191
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HinfI GANTC 6 cut(s) 171, 197, 231, 324, 406, 412
HpaII CCGG 4 cut(s) 5, 228, 358, 382
HphI GGTGA 2 cut(s) 20, 226
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 6 cut(s) 138, 223, 365, 370, 411, 417
Hpy188III TCNNGA 1 cut(s) 201
Hpy8I GTNNAC 1 cut(s) 164
Hpy99I CGWCG 3 cut(s) 155, 165, 227
HpyAV CCTTC 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 2 cut(s) 12, 126
HpyCH4V TGCA 3 cut(s) 54, 66, 284
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 285, 294
HpySE526I ACGT 2 cut(s) 12, 126
Hsp92I GRCGYC 3 cut(s) 12, 153, 225
Hsp92II CATG 2 cut(s) 293, 425
HspAI GCGC 2 cut(s) 30, 191
KpnI GGTACC 1 cut(s) 74
Kzo9I GATC 2 cut(s) 157, 365
LguI GCTCTTC 1 cut(s) 394
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 7 cut(s) 18, 86, 97, 241, 336, 371, 395
Lsp1109I GCAGC 1 cut(s) 281
MaeII ACGT 2 cut(s) 12, 126
MaeIII GTNAC 2 cut(s) 8, 232
MalI GATC 2 cut(s) 159, 367
MboI GATC 2 cut(s) 157, 365
MboII GAAGA 1 cut(s) 411
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 1 cut(s) 365
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 4 cut(s) 61, 318, 371, 393
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 5 cut(s) 165, 240, 318, 400, 406
MmeI TCCRAC 3 cut(s) 193, 246, 343
MnlI CCTC 8 cut(s) 52, 169, 309, 312, 316, 411, 428, 432
Mox20I TGGCCA 1 cut(s) 83
MscI TGGCCA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 87
MspI CCGG 4 cut(s) 5, 228, 358, 382
MspR9I CCNGG 2 cut(s) 6, 359
MunI CAATTG 1 cut(s) 393
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 3 cut(s) 36, 285, 294
NciI CCSGG 2 cut(s) 6, 359
NcoI CCATGG 1 cut(s) 289
NdeII GATC 2 cut(s) 157, 365
NlaIII CATG 2 cut(s) 293, 425
NlaIV GGNNCC 2 cut(s) 72, 367
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 2 cut(s) 8, 232
PaeR7I CTCGAG 1 cut(s) 173
PciSI GCTCTTC 1 cut(s) 394
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 1 cut(s) 296
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 5 cut(s) 165, 239, 318, 400, 406
PpsI GAGTC 5 cut(s) 165, 239, 318, 400, 406
PspN4I GGNNCC 2 cut(s) 72, 367
PspPI GGNCC 1 cut(s) 103
PspXI VCTCGAGB 1 cut(s) 173
PsuI RGATCY 1 cut(s) 365
PvuI CGATCG 1 cut(s) 160
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 125
SalI GTCGAC 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 394
SatI GCNGC 1 cut(s) 295
Sau3AI GATC 2 cut(s) 157, 365
Sau96I GGNCC 1 cut(s) 103
SchI GAGTC 5 cut(s) 165, 240, 318, 400, 406
ScrFI CCNGG 2 cut(s) 6, 359
SetI ASST 7 cut(s) 15, 41, 76, 129, 213, 252, 379
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 4 cut(s) 61, 318, 371, 393
SsiI CCGC 2 cut(s) 87, 330
StyD4I CCNGG 2 cut(s) 4, 357
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 2 cut(s) 15, 129
TaqI TCGA 6 cut(s) 150, 163, 169, 174, 202, 322
TasI AATT 4 cut(s) 61, 318, 371, 393
TfiI GAWTC 1 cut(s) 197
TseFI GTSAC 2 cut(s) 8, 232
TseI GCWGC 1 cut(s) 294
Tsp45I GTSAC 2 cut(s) 8, 232
XapI RAATTY 1 cut(s) 318
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 163
ZraI GACGTC 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.