RLG00000004832

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004869
Physical Location & Seq
Reverse (-)
62868858 .. 62869314
457 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004832

Sequence Viewer

Length: 363 bp
ATGCACAATATTTCAAGATCATACAATGAGGCTGAGACTTGCTTATCACCTTTCTTGGCTGACGGTGTCCCAAATCTGGACCTTGACGATCTTATCATCAACGTGGATGCTTCGGGTGGTGAACTCGACCCCGATGGTAGACTTAAACTCGAGGTTGAACTAGTTTTGCATGAACCTGGACAACAGGTTGGATCTGCCGATGCCGGAATCGCCGATCAAAAGGACCTTGAATCGGTAGTCGTAGTCGTTCGACCTGTAGGCCATGTCGAAGAGCCGGAGAGACTTTCTGGTTGGGGATTTTCGATTGGAGATGAAGAGGCCGCTAGCCTTGTCGTCGTCACCGGAGAAGAAGTCCTCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

12.77

Weight (kDa)

4.05

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 139
AciI CCGC 1 cut(s) 321
AclWI GGATC 1 cut(s) 199
AfiI CCNNNNNNNGG 2 cut(s) 76, 232
AgsI TTSAA 3 cut(s) 15, 158, 230
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 1 cut(s) 175
Alw26I GTCTC 2 cut(s) 29, 274
AlwI GGATC 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 259, 318
AspS9I GGNCC 2 cut(s) 79, 223
AsuHPI GGTGA 3 cut(s) 39, 131, 331
AsuNHI GCTAGC 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 2 cut(s) 79, 223
BccI CCATC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 177
BcoDI GTCTC 2 cut(s) 29, 274
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 2 cut(s) 161, 324
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 2 cut(s) 79, 223
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 2 cut(s) 79, 223
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 2 cut(s) 97, 190
BmtI GCTAGC 1 cut(s) 327
BsaWI WCCGGW 1 cut(s) 341
BsaXI ACNNNNNCTCC 1 cut(s) 336
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 232
BseBI CCWGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 2 cut(s) 76, 232
BseMII CTCAG 1 cut(s) 24
BshFI GGCC 2 cut(s) 261, 320
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 3 cut(s) 204, 275, 342
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 2 cut(s) 76, 232
BsmAI GTCTC 2 cut(s) 29, 274
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 2 cut(s) 261, 320
BsoBI CYCGRG 1 cut(s) 149
Bsp143I GATC 4 cut(s) 17, 88, 191, 214
BspACI CCGC 1 cut(s) 321
BspANI GGCC 2 cut(s) 261, 320
BspCNI CTCAG 1 cut(s) 25
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 1 cut(s) 199
BspQI GCTCTTC 1 cut(s) 264
BssMI GATC 4 cut(s) 17, 88, 191, 214
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 65
Bst6I CTCTTC 2 cut(s) 264, 309
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 112
BstKTI GATC 4 cut(s) 20, 91, 194, 217
BstMAI GTCTC 2 cut(s) 29, 274
BstMBI GATC 4 cut(s) 17, 88, 191, 214
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 191
BstYI RGATCY 1 cut(s) 191
BsuRI GGCC 2 cut(s) 261, 320
BtsCI GGATG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 79, 223
CviAII CATG 2 cut(s) 170, 263
CviJI RGCY 6 cut(s) 32, 59, 261, 274, 320, 327
CviKI_1 RGCY 6 cut(s) 32, 59, 261, 274, 320, 327
DdeI CTNAG 1 cut(s) 33
DpnI GATC 4 cut(s) 19, 90, 193, 216
DpnII GATC 4 cut(s) 17, 88, 191, 214
Eam1104I CTCTTC 2 cut(s) 264, 309
EarI CTCTTC 2 cut(s) 264, 309
Eco47I GGWCC 2 cut(s) 79, 223
Eco88I CYCGRG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 175
FaeI CATG 2 cut(s) 173, 266
FaiI YATR 3 cut(s) 22, 171, 264
FaqI GGGAC 1 cut(s) 53
FatI CATG 2 cut(s) 169, 262
FblI GTMKAC 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 321
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 2 cut(s) 161, 324
GluI GCNGC 1 cut(s) 321
HaeIII GGCC 2 cut(s) 261, 320
HapII CCGG 3 cut(s) 204, 275, 342
Hin1II CATG 2 cut(s) 173, 266
HinfI GANTC 2 cut(s) 207, 230
HpaII CCGG 3 cut(s) 204, 275, 342
HphI GGTGA 3 cut(s) 39, 131, 331
Hpy166II GTNNAC 2 cut(s) 122, 140
Hpy188III TCNNGA 2 cut(s) 15, 77
Hpy8I GTNNAC 2 cut(s) 122, 140
Hpy99I CGWCG 1 cut(s) 338
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 2 cut(s) 4, 169
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 2 cut(s) 173, 266
Kzo9I GATC 4 cut(s) 17, 88, 191, 214
LguI GCTCTTC 1 cut(s) 264
LpnPI CCDG 9 cut(s) 62, 162, 170, 189, 217, 267, 273, 288, 355
LweI GCATC 2 cut(s) 97, 190
MaeI CTAG 2 cut(s) 161, 324
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 337
MalI GATC 4 cut(s) 19, 90, 193, 216
MboI GATC 4 cut(s) 17, 88, 191, 214
MboII GAAGA 3 cut(s) 281, 326, 359
MflI RGATCY 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 3 cut(s) 22, 145, 310
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 101
MspI CCGG 3 cut(s) 204, 275, 342
MspR9I CCNGG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeII GATC 4 cut(s) 17, 88, 191, 214
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 2 cut(s) 173, 266
NmuCI GTSAC 1 cut(s) 337
PaeR7I CTCGAG 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 207, 230
PflFI GACNNNGTC 1 cut(s) 65
PkrI GCNGC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspPI GGNCC 2 cut(s) 79, 223
PspPPI RGGWCCY 1 cut(s) 223
PspXI VCTCGAGB 1 cut(s) 149
PsuI RGATCY 1 cut(s) 191
PsyI GACNNNGTC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 4 cut(s) 17, 88, 191, 214
Sau96I GGNCC 2 cut(s) 79, 223
ScrFI CCNGG 1 cut(s) 177
SetI ASST 8 cut(s) 52, 84, 105, 156, 178, 189, 228, 256
SfaNI GCATC 2 cut(s) 97, 190
SfcI CTRYAG 1 cut(s) 255
Sfr274I CTCGAG 1 cut(s) 149
SinI GGWCC 2 cut(s) 79, 223
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SpeI ACTAGT 1 cut(s) 160
SsiI CCGC 1 cut(s) 321
SspI AATATT 1 cut(s) 10
SspMI CTAG 2 cut(s) 161, 324
StyD4I CCNGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 1 cut(s) 105
TaqI TCGA 5 cut(s) 126, 150, 250, 267, 302
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 2 cut(s) 207, 230
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 1 cut(s) 337
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 2 cut(s) 186, 327
Tth111I GACNNNGTC 1 cut(s) 65
VpaK11BI GGWCC 2 cut(s) 79, 223
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 139
XspI CTAG 2 cut(s) 161, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.