Rmu_sc0008535.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008535.1
OG0004869
Physical Location & Seq
Reverse (-)
1 .. 7000
7000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008535.1_g000001.1.cds

Sequence Viewer

Length: 775 bp
atggagattccggtggccggttcggtaagtttccagccggttctttgggtggtgccgcctgttctggactcctggggtcgagtacttcagggtgtgaggtggaggcagaaaaggcttcaaggttttgttttcggattcaagtttttcggtcatccagaagtgtatattcccattctgcctagatctggactggatggaaattctccactttcagctcctcaaatgaatgaagtcgtcttggttttggatcccagcactattttgatttcttcctcttcctcttgttcttcttcctcttcctcttccccgtgctgctctgctccaattttcaaatgccgaaactactctccaccaccagcaccaccccacccacccaacccaacaccagtcccaccacctcaacaaccacacccactactcgccgccgccgcctcaaccccacagactccgcttcaaccgcctccgcctcctccgcggagtccaagctccgagagcacgtggacctccacagcctccaccgccatgccgccacctccgccgccgccgtcgtccacgtgggatttctgggacccttttgtaccggcttcgtctcagtcggtgatcgaggaggaatgggaggctacgaccaccgcgtcggaggcgatggttacgactgtcaccgtggcggcgagcaccgtggcgccgccttcggttgttagtgggttttcgaaggacagtgaggaagaggaagaagaacaagaggaagaggaagaactgaagaaggagaaccgaaagg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

27.76

Weight (kDa)

4.67

Isoelectric Point (pI)

95.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 631
AccB1I GGYRCC 2 cut(s) 52, 679
AccII CGCG 2 cut(s) 475, 632
AclWI GGATC 2 cut(s) 242, 255
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 71
AcvI CACGTG 2 cut(s) 498, 555
AcyI GRCGYC 1 cut(s) 680
AfaI GTAC 2 cut(s) 84, 579
AfiI CCNNNNNNNGG 2 cut(s) 17, 185
AgsI TTSAA 4 cut(s) 119, 139, 331, 455
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 2 cut(s) 215, 486
AluI AGCT 2 cut(s) 215, 486
Alw21I GWGCWC 2 cut(s) 497, 674
Alw26I GTCTC 1 cut(s) 594
AlwI GGATC 2 cut(s) 242, 255
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 1 cut(s) 199
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 2 cut(s) 501, 568
AsuHPI GGTGA 2 cut(s) 610, 649
AsuII TTCGAA 1 cut(s) 707
AvaII GGWCC 2 cut(s) 501, 568
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 2 cut(s) 52, 679
BbrPI CACGTG 2 cut(s) 498, 555
Bbv12I GWGCWC 2 cut(s) 497, 674
BbvI GCAGC 1 cut(s) 299
BccI CCATC 2 cut(s) 188, 637
BceAI ACGGC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 594
BfaI CTAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 683
BglII AGATCT 1 cut(s) 182
BmcAI AGTACT 1 cut(s) 84
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 2 cut(s) 501, 568
BmgT120I GGNCC 2 cut(s) 501, 568
BmiI GGNNCC 5 cut(s) 54, 249, 569, 570, 681
BmrFI CCNGG 1 cut(s) 73
Bpu14I TTCGAA 1 cut(s) 707
BsaAI YACGTR 2 cut(s) 498, 555
BsaHI GRCGYC 1 cut(s) 680
BsaJI CCNNGG 4 cut(s) 72, 473, 660, 675
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 185
Bse118I RCCGGY 3 cut(s) 17, 37, 580
Bse1I ACTGG 2 cut(s) 195, 386
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 4 cut(s) 72, 473, 660, 675
BseGI GGATG 2 cut(s) 151, 199
BseLI CCNNNNNNNGG 2 cut(s) 17, 185
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 2 cut(s) 195, 386
BseRI GAGGAG 3 cut(s) 207, 459, 620
BseXI GCAGC 1 cut(s) 299
BseYI CCCAGC 1 cut(s) 251
Bsh1236I CGCG 2 cut(s) 475, 632
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 2 cut(s) 52, 679
BsiHKAI GWGCWC 2 cut(s) 497, 674
BsiSI CCGG 4 cut(s) 11, 18, 38, 581
BslFI GGGAC 2 cut(s) 374, 581
BslI CCNNNNNNNGG 2 cut(s) 17, 185
BsmAI GTCTC 1 cut(s) 594
BsmBI CGTCTC 1 cut(s) 594
BsmFI GGGAC 2 cut(s) 374, 581
BsnI GGCC 1 cut(s) 17
Bsp119I TTCGAA 1 cut(s) 707
Bsp1286I GDGCHC 2 cut(s) 497, 674
Bsp143I GATC 3 cut(s) 182, 247, 600
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 1 cut(s) 604
BspFNI CGCG 2 cut(s) 475, 632
BspLI GGNNCC 5 cut(s) 54, 249, 569, 570, 681
BspPI GGATC 2 cut(s) 242, 255
BspT104I TTCGAA 1 cut(s) 707
BspT107I GGYRCC 2 cut(s) 52, 679
BsrFI RCCGGY 3 cut(s) 17, 37, 580
BsrI ACTGG 2 cut(s) 195, 386
BssAI RCCGGY 3 cut(s) 17, 37, 580
BssECI CCNNGG 4 cut(s) 72, 473, 660, 675
BssMI GATC 3 cut(s) 182, 247, 600
BssNI GRCGYC 1 cut(s) 680
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 4 cut(s) 655, 661, 676, 716
Bst6I CTCTTC 5 cut(s) 280, 301, 307, 717, 738
BstACI GRCGYC 1 cut(s) 680
BstBAI YACGTR 2 cut(s) 498, 555
BstBI TTCGAA 1 cut(s) 707
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 1 cut(s) 591
BstDSI CCRYGG 3 cut(s) 473, 660, 675
BstF5I GGATG 2 cut(s) 151, 199
BstFNI CGCG 2 cut(s) 475, 632
BstH2I RGCGCY 1 cut(s) 683
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 3 cut(s) 185, 250, 603
BstMAI GTCTC 1 cut(s) 594
BstMBI GATC 3 cut(s) 182, 247, 600
BstMWI GCNNNNNNNGC 8 cut(s) 112, 428, 457, 472, 492, 518, 535, 638
BstNI CCWGG 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 71
BstUI CGCG 2 cut(s) 475, 632
BstV1I GCAGC 1 cut(s) 299
BstX2I RGATCY 2 cut(s) 182, 247
BstYI RGATCY 2 cut(s) 182, 247
BsuRI GGCC 1 cut(s) 17
BtgI CCRYGG 3 cut(s) 473, 660, 675
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 2 cut(s) 151, 199
BtsIMutI CAGTG 1 cut(s) 721
Cac8I GCNNGC 1 cut(s) 670
CfoI GCGC 1 cut(s) 682
Cfr10I RCCGGY 3 cut(s) 17, 37, 580
Cfr13I GGNCC 2 cut(s) 501, 568
Cfr42I CCGCGG 1 cut(s) 476
CseI GACGC 1 cut(s) 621
Csp6I GTAC 2 cut(s) 83, 578
CviAII CATG 1 cut(s) 523
CviJI RGCY 8 cut(s) 17, 37, 115, 215, 486, 512, 584, 620
CviKI_1 RGCY 8 cut(s) 17, 37, 115, 215, 486, 512, 584, 620
CviQI GTAC 2 cut(s) 83, 578
DdeI CTNAG 1 cut(s) 591
DinI GGCGCC 1 cut(s) 681
DpnI GATC 3 cut(s) 184, 249, 602
DpnII GATC 3 cut(s) 182, 247, 600
DrdI GACNNNNNNGTC 1 cut(s) 631
DseDI GACNNNNNNGTC 1 cut(s) 631
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 5 cut(s) 280, 301, 307, 717, 738
EarI CTCTTC 5 cut(s) 280, 301, 307, 717, 738
EciI GGCGGA 2 cut(s) 453, 525
Eco47I GGWCC 2 cut(s) 501, 568
Eco57I CTGAAG 1 cut(s) 71
Eco72I CACGTG 2 cut(s) 498, 555
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 71
EgeI GGCGCC 1 cut(s) 681
EheI GGCGCC 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 594
FaeI CATG 1 cut(s) 526
FaiI YATR 2 cut(s) 165, 524
FaqI GGGAC 2 cut(s) 374, 581
FatI CATG 1 cut(s) 522
FokI GGATG 2 cut(s) 138, 206
FspBI CTAG 1 cut(s) 180
GlaI GCGC 1 cut(s) 681
GsaI CCCAGC 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 683
HaeIII GGCC 1 cut(s) 17
HapII CCGG 4 cut(s) 11, 18, 38, 581
HgaI GACGC 1 cut(s) 621
HhaI GCGC 1 cut(s) 682
Hin1I GRCGYC 1 cut(s) 680
Hin1II CATG 1 cut(s) 526
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HinfI GANTC 5 cut(s) 7, 68, 135, 445, 478
HpaII CCGG 4 cut(s) 11, 18, 38, 581
HphI GGTGA 2 cut(s) 610, 649
Hpy166II GTNNAC 2 cut(s) 501, 552
Hpy188I TCNGA 3 cut(s) 134, 490, 637
Hpy188III TCNNGA 3 cut(s) 65, 155, 186
Hpy8I GTNNAC 2 cut(s) 501, 552
Hpy99I CGWCG 2 cut(s) 550, 637
HpyAV CCTTC 3 cut(s) 696, 703, 754
HpyCH4III ACNGT 4 cut(s) 655, 661, 676, 716
HpyCH4IV ACGT 2 cut(s) 497, 554
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 428, 457, 472, 492, 518, 535, 638
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 2 cut(s) 497, 554
Hsp92I GRCGYC 1 cut(s) 680
Hsp92II CATG 1 cut(s) 526
HspAI GCGC 1 cut(s) 680
KasI GGCGCC 1 cut(s) 679
KflI GGGWCCC 1 cut(s) 568
KspI CCGCGG 1 cut(s) 476
Kzo9I GATC 3 cut(s) 182, 247, 600
LmnI GCTCC 3 cut(s) 220, 325, 491
Lsp1109I GCAGC 1 cut(s) 299
MaeI CTAG 1 cut(s) 180
MaeII ACGT 2 cut(s) 497, 554
MaeIII GTNAC 2 cut(s) 646, 655
MalI GATC 3 cut(s) 184, 249, 602
MboI GATC 3 cut(s) 182, 247, 600
MflI RGATCY 2 cut(s) 182, 247
MhlI GDGCHC 2 cut(s) 497, 674
MluCI AATT 2 cut(s) 199, 324
Mly113I GGCGCC 1 cut(s) 680
MlyI GAGTC 3 cut(s) 62, 439, 487
MmeI TCCRAC 1 cut(s) 615
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 4 cut(s) 11, 18, 38, 581
MspR9I CCNGG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 73
MvnI CGCG 2 cut(s) 475, 632
MwoI GCNNNNNNNGC 8 cut(s) 112, 428, 457, 472, 492, 518, 535, 638
NarI GGCGCC 1 cut(s) 680
NdeII GATC 3 cut(s) 182, 247, 600
NlaIII CATG 1 cut(s) 526
NlaIV GGNNCC 5 cut(s) 54, 249, 569, 570, 681
NmuCI GTSAC 1 cut(s) 655
NspV TTCGAA 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 629
PfeI GAWTC 2 cut(s) 7, 135
PleI GAGTC 3 cut(s) 62, 439, 486
PluTI GGCGCC 1 cut(s) 683
PmaCI CACGTG 2 cut(s) 498, 555
PmlI CACGTG 2 cut(s) 498, 555
PpsI GAGTC 3 cut(s) 62, 439, 486
Ppu21I YACGTR 2 cut(s) 498, 555
PpuMI RGGWCCY 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 71
PspCI CACGTG 2 cut(s) 498, 555
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 5 cut(s) 54, 249, 569, 570, 681
PspPI GGNCC 2 cut(s) 501, 568
PspPPI RGGWCCY 1 cut(s) 568
PsuI RGATCY 2 cut(s) 182, 247
RsaI GTAC 2 cut(s) 84, 579
RsaNI GTAC 2 cut(s) 83, 578
RseI CAYNNNNRTG 1 cut(s) 521
SacII CCGCGG 1 cut(s) 476
Sau3AI GATC 3 cut(s) 182, 247, 600
Sau96I GGNCC 2 cut(s) 501, 568
ScaI AGTACT 1 cut(s) 84
SchI GAGTC 3 cut(s) 62, 439, 487
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 2 cut(s) 497, 674
SetI ASST 9 cut(s) 101, 124, 217, 400, 488, 500, 506, 535, 557
SfoI GGCGCC 1 cut(s) 681
Sfr303I CCGCGG 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 707
SgrBI CCGCGG 1 cut(s) 476
SinI GGWCC 2 cut(s) 501, 568
SmiMI CAYNNNNRTG 1 cut(s) 521
Sse9I AATT 2 cut(s) 199, 324
SspDI GGCGCC 1 cut(s) 679
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 4 cut(s) 655, 661, 676, 716
TaiI ACGT 2 cut(s) 500, 557
TaqI TCGA 3 cut(s) 79, 603, 707
TaqII GACCGA 1 cut(s) 137
TasI AATT 2 cut(s) 199, 324
TatI WGTACW 1 cut(s) 82
TauI GCSGC 9 cut(s) 58, 425, 428, 431, 529, 541, 544, 668, 685
TfiI GAWTC 2 cut(s) 7, 135
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 1 cut(s) 312
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 2 cut(s) 239, 243
TspRI CASTG 1 cut(s) 721
VpaK11BI GGWCC 2 cut(s) 501, 568
XapI RAATTY 1 cut(s) 199
XspI CTAG 1 cut(s) 180
ZrmI AGTACT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.