RLG00000020604

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004869
Physical Location & Seq
Reverse (-)
66675594 .. 66676933
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020604

Sequence Viewer

Length: 438 bp
ATGGAATTGAAAAGCTTATCACATTTCTTGGCCGGCGGTGTCCCAAATCTGGGCCTTGACGATCTAATCATCGATGTGGATGCTCCGAGTGGCAAACTTGACGCCGATGGTGGACTTAGACTCAAGGTTGAACTTGTTGTGCATGAACCTGGACAACAAATACAATTTGCCGATGCCGGAATCGTCGATCAAGACGACCTTGAAGTGGTAGTCATTGTCCAACTTGGTTTCGATGCATGTGAGGTGGAGGAAGAGGATATGGTGGTGGTGAGGAAGGGAGCTGCCAGAGTCGAGGCGCGTGAGGCATCTCGAGTAGAAATTGGAGATCTGACTCAGACTATCAACTTGAAGGAGCAGAATCCTGACACTGTCGCCGCCACCGTGGTCGATGGTTTGCTGGCTGCACAATCCGTAATTTCAGGAAACTTCTTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.41

Weight (kDa)

4.14

Isoelectric Point (pI)

21.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 298
AciI CCGC 2 cut(s) 36, 375
AcoI YGGCCR 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 102
AfiI CCNNNNNNNGG 3 cut(s) 49, 50, 205
AgsI TTSAA 4 cut(s) 10, 131, 203, 349
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 2 cut(s) 15, 281
AluI AGCT 2 cut(s) 15, 281
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 2 cut(s) 30, 52
ApeKI GCWGC 2 cut(s) 281, 401
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 309
BbvI GCAGC 2 cut(s) 268, 388
BccI CCATC 2 cut(s) 101, 383
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 150
BglII AGATCT 1 cut(s) 325
BisI GCNGC 3 cut(s) 282, 375, 402
BlsI GCNGC 3 cut(s) 283, 376, 403
Bme1390I CCNGG 1 cut(s) 150
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 4 cut(s) 70, 163, 223, 314
BpuEI CTTGAG 1 cut(s) 107
Bsa29I ATCGAT 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 381
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 50, 205
Bse118I RCCGGY 1 cut(s) 32
BseBI CCWGG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 3 cut(s) 49, 50, 205
BseMII CTCAG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 268, 388
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 2 cut(s) 32, 54
BshVI ATCGAT 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 2 cut(s) 33, 177
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 3 cut(s) 49, 50, 205
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 2 cut(s) 32, 54
BsoBI CYCGRG 1 cut(s) 309
Bsp143I GATC 3 cut(s) 61, 187, 325
BspACI CCGC 2 cut(s) 36, 375
BspANI GGCC 2 cut(s) 32, 54
BspCNI CTCAG 1 cut(s) 346
BspDI ATCGAT 1 cut(s) 72
BspFNI CGCG 1 cut(s) 298
BspHI TCATGA 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 3 cut(s) 61, 187, 325
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 370, 382
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 34, 399
BstDEI CTNAG 2 cut(s) 116, 333
BstDSI CCRYGG 1 cut(s) 381
BstF5I GGATG 1 cut(s) 85
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 3 cut(s) 64, 190, 328
BstMBI GATC 3 cut(s) 61, 187, 325
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 148
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 2 cut(s) 268, 388
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
Bsu15I ATCGAT 1 cut(s) 72
BsuRI GGCC 2 cut(s) 32, 54
BsuTUI ATCGAT 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 34, 399
CciI TCATGA 1 cut(s) 434
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 72
CseI GACGC 1 cut(s) 110
CviAII CATG 3 cut(s) 143, 237, 435
CviJI RGCY 5 cut(s) 15, 32, 54, 281, 401
CviKI_1 RGCY 5 cut(s) 15, 32, 54, 281, 401
DdeI CTNAG 2 cut(s) 116, 333
DpnI GATC 3 cut(s) 63, 189, 327
DpnII GATC 3 cut(s) 61, 187, 325
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 3 cut(s) 146, 240, 438
FaiI YATR 4 cut(s) 144, 238, 260, 436
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FaqI GGGAC 1 cut(s) 26
FatI CATG 3 cut(s) 142, 236, 434
Fnu4HI GCNGC 3 cut(s) 282, 375, 402
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 3 cut(s) 282, 375, 402
GlaI GCGC 1 cut(s) 297
GluI GCNGC 3 cut(s) 282, 375, 402
HaeIII GGCC 2 cut(s) 32, 54
HapII CCGG 2 cut(s) 33, 177
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 146, 240, 438
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 5 cut(s) 120, 180, 288, 331, 358
HpaII CCGG 2 cut(s) 33, 177
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 3 cut(s) 87, 330, 336
Hpy188III TCNNGA 5 cut(s) 191, 309, 362, 420, 435
Hpy8I GTNNAC 1 cut(s) 113
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 2 cut(s) 268, 343
HpyCH4III ACNGT 2 cut(s) 370, 382
HpyCH4V TGCA 3 cut(s) 142, 236, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 2 cut(s) 116, 333
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 146, 240, 438
HspAI GCGC 1 cut(s) 296
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 3 cut(s) 61, 187, 325
LmnI GCTCC 3 cut(s) 88, 278, 352
LpnPI CCDG 9 cut(s) 35, 46, 135, 162, 190, 298, 375, 383, 405
Lsp1109I GCAGC 2 cut(s) 268, 388
LweI GCATC 4 cut(s) 70, 163, 223, 314
MalI GATC 3 cut(s) 63, 189, 327
MboI GATC 3 cut(s) 61, 187, 325
MboII GAAGA 1 cut(s) 263
MflI RGATCY 1 cut(s) 325
MluCI AATT 4 cut(s) 5, 164, 318, 414
MlyI GAGTC 3 cut(s) 114, 297, 325
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 6 cut(s) 235, 241, 247, 264, 286, 295
Mph1103I ATGCAT 1 cut(s) 238
MroNI GCCGGC 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 74
MspI CCGG 2 cut(s) 33, 177
MspR9I CCNGG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 1 cut(s) 302
NaeI GCCGGC 1 cut(s) 34
NdeII GATC 3 cut(s) 61, 187, 325
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 3 cut(s) 146, 240, 438
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 240
PaeR7I CTCGAG 1 cut(s) 309
PagI TCATGA 1 cut(s) 434
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 378
PdiI GCCGGC 1 cut(s) 34
PfeI GAWTC 2 cut(s) 180, 358
PflFI GACNNNGTC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 283, 376, 403
PleI GAGTC 3 cut(s) 114, 296, 325
PpsI GAGTC 3 cut(s) 114, 296, 325
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspPI GGNCC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 325
PsyI GACNNNGTC 1 cut(s) 368
RseI CAYNNNNRTG 1 cut(s) 74
SatI GCNGC 3 cut(s) 282, 375, 402
Sau3AI GATC 3 cut(s) 61, 187, 325
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 3 cut(s) 114, 297, 325
ScrFI CCNGG 1 cut(s) 150
SetI ASST 6 cut(s) 17, 129, 151, 201, 246, 283
SfaNI GCATC 4 cut(s) 70, 163, 223, 314
Sfr274I CTCGAG 1 cut(s) 309
SlaI CTCGAG 1 cut(s) 309
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 122, 309
SmoI CTYRAG 2 cut(s) 122, 309
Sse9I AATT 4 cut(s) 5, 164, 318, 414
SsiI CCGC 2 cut(s) 36, 375
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 370, 382
TaqI TCGA 6 cut(s) 72, 186, 231, 291, 310, 387
TasI AATT 4 cut(s) 5, 164, 318, 414
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 2 cut(s) 180, 358
TscAI CASTG 1 cut(s) 373
TseI GCWGC 2 cut(s) 281, 401
TspDTI ATGAA 2 cut(s) 159, 423
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 1 cut(s) 373
Tth111I GACNNNGTC 1 cut(s) 368
XceI RCATGY 1 cut(s) 240
XhoI CTCGAG 1 cut(s) 309
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.