Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002245

Physical Location & Seq

Genomic Coordinates Forward (+)

8752675 .. 8755095

Gene Structure 2421 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1121900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGGAGATTGGAACTTCATTCATTTGAACCATTTCACGGGCTTCATATTTTTGTCTGTTATTCTTCTCGCTTCCGAAACTTGCCTGGCTCTTGTTCGAAATTTTGGCACAATTAATCCGCCGTTTCAAGGGGCCCAGATAAATTGGATCGATAATGGCGGGTTGTTTCTCTTGTCTAACCGGTCCGAATTCGGTTTTGGCTTCAAAAGATTCGAAACTAGTCCCCAAGATCGATTATTTCTGCTTGTTATTGTTCACATGGACAGTTCGAGGGTAGTCTGGACCGGGAATAGGGGTTCCCCGGTTTTAAATTCTGATAAATTTGTGTTTGATGCTAAGAGTGGTGTGTTCTTGCAAAGCGACGGAGGTGTGGTTTGGTCTGCAGATACTGGTGGCAAAAGAGTATCTGCAATAGAGTTGCAGGACTCTGGAAATTTGGTTTTGCATGGTGATGACGGTGGAGTAGTTTGGCAGAGTTTTAGCCATCCAACTGATACCCTGTTATGGAACCAGGAATATACGGAAGGAATGAAGCTGGTAAGCAATCCTAGCTCCAACAACTTGAGTTGTATTCTTCACATCAAGTCTGGAGACTTAGTTCTTTCAGCAGGTTACCAAACACCACAGCCGTATTGGTCTATGACGAAAGAAAACCGTAAAACAATCAACAAAGACGGTGGTGTAGTCACTTCAGCTACTATCAGTGAAAATTCATGGAAGTTCTTTGATCGATCCAAAGCGTTGCTATGGCAATTTATTTTCTCAAGCAATATTGATTCAAATGCCACATGGATTGCAGTTTTAGGAAGTGATGGATTTATCTCTTTCTACAATCTTAGAGGGTCAATTGATCCTTCAGGAACAAAAATACCAAGCGATTCGTGCAGCACACCAGAACCTTGTGATTCATACTTCGAGTGTTTTAGTAACAAGAACTGCCAGTGCCCTTCAGGTCTCAGCTCGCGTCGTAATTGCAAGGCGGGGGTTGTCTCCTCATGTGATCCTTCAAGGGGTTCTACAGAGCTTGTAAATGCTGGGGACGGGCTCTATTATTTTGCGTTGGGATATATTCCAGCTTCTTCAAAAACTGATTTGAACGGTTGCCAAACGTCTTGCCTTGGCAACTGTTCGTGTGTCGCTCTGTTCTTCCAAAACAGTACAAGAAATTGTTTTCTGTTTGACAAATTAGGTAGCTTCCAAAATTCTGACAAGGGCTCTGGCTTTGTTTCTTATATTAAAGTCTTGAGGGATGGAAGCAGTGGTACTGGGGCGAGCAGAAGCAGCAAGAAGCGCTTCCCTTATGTTGTGATCATAGCCATCTCGACAGTACTTGCAATTATTTGTGGCTTACTTTATATGGGATGCCGATACTACTGGAAGAAGAGAAAATCGCCAGAGTCTCCTACGGAGACATCAGAAGAAGGAAATTTCTTTGAAAATTTGACTGGGAAGCCCATCCGTTTCAGTTACAAAGATCTTCAAACTGCAACTAATAACTTCTCGGTGAAGCTAGGGCAAGGAGGTTTTGGCTCGGTTTACCACGGGATTCTCCCAGATGGGACTCGACTTGCTGTGAAGAAGTTGGAAGGCATCGGTCAGGGACAGAAAGAGTTTCAAGCTGAAGTTACCATCATTGGCAGTATCCATCATCTGCACTTGGTTCGGCTCAGGGGCTTCTGCGCAGAAGGAAGCCATCGACTTCTTGCTTACGATTACATGGCAAATGGTTCTCTTGATAAATGGATTTTCAAAAAATACAACAAAGAATCCCTGTTGGATTGGGAGACAAGGTTCAATATAGCATTGTGTACAGCTAAAGGACTAGCTTATCTCCACGAAGATTGCGATTCAAAGATCGTTCACTGTGACATAAAACCTGAAAATGTTCTCCTAGACGATAATTACCTTGCCAAAGTCGCTGACTTTGGTTTGGCAAAGCTAATGACTAGGGGGCAGAGCCATGTTTTCACAACACTAAGAGGAACCCGGGGTTACCTTGCACCAGAGTGGATCAACAACTCCCCTATATCAGAGAAGATCGATGTGTATAGCTATGGAATGCTGTTGCTAGAGGTCATTGGTGGGAGAAAGAACTATGACCGGACAGAAACTTCTGAAAAGTCAAATTTTCCATCCTATGCCTTCAAGATGTTGGGAGAAGGAAAACTGAAGGATATCGTTGATACAAAGTTAGGGATAGATAAAGGCGACAAGAGGGTTGAGACAGCCATTATGGTTGCACTATGGTGCATACAGGAAGATATGTCCTTGAGACCATCGATGACTAAGGTTGTCCAGATGCTTGAAGGCCTCCTTCCTGTTCCTCAGCCTCCAACCTCCGCCGCAAGGGGATCTCAACTTTATACAGACGTCTTTAAATCAGACAACAATTCTGCAGTGTGCCTTTCAGGCCCAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0031625 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044464 GO:0046777 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr

Protein Analysis

807

Amino Acids

89.08

Weight (kDa)

8.07

Isoelectric Point (pI)

39.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	92 - 172	6.3e-14	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	501 - 767	9.6e-39	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	502 - 766	4.7e-49	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002245)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32300
fragaria_vesca	FvH4_2g29050 FvH4_2g29070
malus_domestica	MD00G1144200.v1.1 MD15G1121900.v1.1 MD15G1122800.v1.1 MD15G1125500.v1.1
prunus_persica	Prupe.1G481100_v2.0.a1
pyrus_communis	pycom15g10990 pycom15g11300
rosa_chinensis	RchiOBHm_Chr4g0385931 RchiOBHm_Chr6g0297921 RchiOBHm_Chr6g0297931
rosa_laevigata	RLG00000010199 RLG00000011550 RLG00000011551
rosa_multiflora	Rmu_co8186410.1_g000001 Rmu_sc0007302.1_g000003 Rmu_sc0007302.1_g000004 Rmu_sc0007400.1_g000011 Rmu_sc0011097.1_g000002 Rmu_sc0011097.1_g000003 Rmu_sc0011097.1_g000004 Rmu_sc0015868.1_g000001 Rmu_sc0030316.1_g000001
rosa_roxburghii	Rroxscaffold_5G00333530 Rroxscaffold_7G00170060 Rroxscaffold_7G00178900 Rroxscaffold_7G00178910
rosa_rugosa	Rorug03G0303100 Rorug06G0276800
rosa_samantha	Rh4CG008200 Rh4DG007100 Rh6AG387500 Rh6BG395900 Rh6CG401600 Rh6CG401700 Rh6CG401800 Rh6DG388100
rosa_wichuraiana	Rw4G000320 Rw6G033760 Rw6G033810 Rw6G033820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2373
Acc16I	TGCGCA	1 cut(s)	1681
Acc36I	ACCTGC	1 cut(s)	599
AccII	CGCG	1 cut(s)	964
AciI	CCGC	5 cut(s)	119, 159, 980, 2340, 2343
AclWI	GGATC	6 cut(s)	155, 726, 845, 995, 2018, 2359
AcuI	CTGAAG	5 cut(s)	675, 840, 933, 1641, 2189
AcyI	GRCGYC	1 cut(s)	2370
AfaI	GTAC	4 cut(s)	1159, 1264, 1329, 1810
AfeI	AGCGCT	1 cut(s)	1292
AfiI	CCNNNNNNNGG	6 cut(s)	37, 128, 291, 504, 1010, 2346
AgeI	ACCGGT	1 cut(s)	180
AhlI	ACTAGT	1 cut(s)	218
AjnI	CCWGG	2 cut(s)	84, 510
AjuI	GAANNNNNNNTTGG	2 cut(s)	180, 212
AleI	CACNNNNGTG	2 cut(s)	2005, 2245
AloI	GAACNNNNNNTCC	6 cut(s)	280, 312, 582, 614, 1775, 1807
Alw26I	GTCTC	8 cut(s)	585, 959, 994, 1403, 1404, 1778, 2216, 2266
AlwI	GGATC	6 cut(s)	155, 726, 845, 995, 2018, 2359
AlwNI	CAGNNNCTG	1 cut(s)	389
Ama87I	CYCGRG	1 cut(s)	1986
Aor51HI	AGCGCT	1 cut(s)	1292
AoxI	GGCC	3 cut(s)	132, 2308, 2410
ApaI	GGGCCC	1 cut(s)	136
ApeKI	GCWGC	2 cut(s)	885, 1281
AseI	ATTAAT	1 cut(s)	114
AsiGI	ACCGGT	1 cut(s)	180
Asp700I	GAANNNNTTC	3 cut(s)	32, 1292, 1884
AspLEI	GCGC	2 cut(s)	1293, 1682
AspS9I	GGNCC	5 cut(s)	132, 133, 183, 282, 2411
AsuC2I	CCSGG	4 cut(s)	286, 302, 1987, 1988
AsuHPI	GGTGA	2 cut(s)	461, 1516
AsuII	TTCGAA	2 cut(s)	97, 213
AvaI	CYCGRG	1 cut(s)	1986
AvaII	GGWCC	2 cut(s)	183, 282
BaeGI	GKGCMC	2 cut(s)	136, 947
BanII	GRGCYC	3 cut(s)	136, 1047, 1217
BarI	GAAGNNNNNNTAC	2 cut(s)	1246, 1278
BbvCI	CCTCAGC	1 cut(s)	2325
BbvI	GCAGC	2 cut(s)	897, 1293
BceAI	ACGGC	2 cut(s)	106, 613
BcgI	CGANNNNNNTGC	2 cut(s)	1643, 1677
BciT130I	CCWGG	2 cut(s)	86, 512
BciVI	GTATCC	1 cut(s)	1652
BclI	TGATCA	1 cut(s)	1308
BcnI	CCSGG	4 cut(s)	286, 302, 1987, 1988
BcoDI	GTCTC	8 cut(s)	585, 959, 994, 1403, 1404, 1778, 2216, 2266
BcuI	ACTAGT	1 cut(s)	218
BfaI	CTAG	7 cut(s)	219, 549, 1511, 1823, 1892, 1947, 2069
BfmI	CTRYAG	3 cut(s)	381, 1017, 2394
BfoI	RGCGCY	1 cut(s)	1294
BfuAI	ACCTGC	1 cut(s)	599
BfuI	GTATCC	1 cut(s)	1652
BglI	GCCNNNNNGGC	1 cut(s)	2409
BglII	AGATCT	1 cut(s)	1474
BisI	GCNGC	3 cut(s)	886, 1282, 2343
BlsI	GCNGC	3 cut(s)	887, 1283, 2344
BmcAI	AGTACT	1 cut(s)	1329
Bme1390I	CCNGG	6 cut(s)	86, 286, 302, 512, 1987, 1988
Bme18I	GGWCC	2 cut(s)	183, 282
BmeT110I	CYCGRG	1 cut(s)	1986
BmgT120I	GGNCC	5 cut(s)	132, 133, 183, 282, 2411
BmiI	GGNNCC	5 cut(s)	133, 134, 298, 509, 1984
BmrFI	CCNGG	6 cut(s)	86, 286, 302, 512, 1987, 1988
BmrI	ACTGGG	2 cut(s)	1275, 1455
BmsI	GCATC	4 cut(s)	322, 1352, 1599, 2289
BmuI	ACTGGG	2 cut(s)	1275, 1455
BpmI	CTGGAG	1 cut(s)	609
Bpu10I	CCTNAGC	2 cut(s)	1667, 2325
Bpu14I	TTCGAA	2 cut(s)	97, 213
BpuEI	CTTGAG	4 cut(s)	583, 748, 1264, 2290
BpuMI	CCSGG	4 cut(s)	286, 302, 1987, 1988
Bsa29I	ATCGAT	5 cut(s)	150, 232, 730, 2040, 2279
BsaHI	GRCGYC	1 cut(s)	2370
BsaI	GGTCTC	2 cut(s)	959, 2266
BsaJI	CCNNGG	5 cut(s)	300, 1117, 1540, 1986, 1987
BsaWI	WCCGGW	2 cut(s)	180, 2100
BsaXI	ACNNNNNCTCC	6 cut(s)	357, 387, 582, 612, 1775, 1805
Bsc4I	CCNNNNNNNGG	6 cut(s)	37, 128, 291, 504, 1010, 2346
Bse118I	RCCGGY	1 cut(s)	180
Bse1I	ACTGG	5 cut(s)	394, 940, 1270, 1379, 1450
BseBI	CCWGG	2 cut(s)	86, 512
BseCI	ATCGAT	5 cut(s)	150, 232, 730, 2040, 2279
BseDI	CCNNGG	5 cut(s)	300, 1117, 1540, 1986, 1987
BseGI	GGATG	5 cut(s)	484, 1255, 1367, 1455, 2132
BseLI	CCNNNNNNNGG	6 cut(s)	37, 128, 291, 504, 1010, 2346
BseMII	CTCAG	3 cut(s)	970, 1681, 2339
BseNI	ACTGG	5 cut(s)	394, 940, 1270, 1379, 1450
BseRI	GAGGAG	1 cut(s)	982
BseSI	GKGCMC	2 cut(s)	136, 947
BseXI	GCAGC	2 cut(s)	897, 1293
BseYI	CCCAGC	1 cut(s)	1034
BsgI	GTGCAG	2 cut(s)	904, 1637
Bsh1236I	CGCG	1 cut(s)	964
BshFI	GGCC	3 cut(s)	134, 2310, 2412
BshTI	ACCGGT	1 cut(s)	180
BshVI	ATCGAT	5 cut(s)	150, 232, 730, 2040, 2279
BsiHKCI	CYCGRG	1 cut(s)	1986
BsiSI	CCGG	5 cut(s)	181, 285, 302, 1987, 2101
BslFI	GGGAC	4 cut(s)	207, 1052, 1573, 1614
BslI	CCNNNNNNNGG	6 cut(s)	37, 128, 291, 504, 1010, 2346
BsmAI	GTCTC	8 cut(s)	585, 959, 994, 1403, 1404, 1778, 2216, 2266
BsmFI	GGGAC	4 cut(s)	207, 1052, 1573, 1614
BsmI	GAATGC	1 cut(s)	2064
BsnI	GGCC	3 cut(s)	134, 2310, 2412
Bso31I	GGTCTC	2 cut(s)	959, 2266
BsoBI	CYCGRG	1 cut(s)	1986
Bsp119I	TTCGAA	2 cut(s)	97, 213
Bsp120I	GGGCCC	1 cut(s)	132
Bsp1286I	GDGCHC	4 cut(s)	136, 947, 1047, 1217
Bsp1407I	TGTACA	1 cut(s)	1808
BspACI	CCGC	5 cut(s)	119, 159, 980, 2340, 2343
BspANI	GGCC	3 cut(s)	134, 2310, 2412
BspCNI	CTCAG	3 cut(s)	969, 1680, 2338
BspDI	ATCGAT	5 cut(s)	150, 232, 730, 2040, 2279
BspFNI	CGCG	1 cut(s)	964
BspLI	GGNNCC	5 cut(s)	133, 134, 298, 509, 1984
BspMAI	CTGCAG	2 cut(s)	385, 2398
BspMI	ACCTGC	1 cut(s)	599
BspPI	GGATC	6 cut(s)	155, 726, 845, 995, 2018, 2359
BspT104I	TTCGAA	2 cut(s)	97, 213
BspTNI	GGTCTC	2 cut(s)	959, 2266
BsrFI	RCCGGY	1 cut(s)	180
BsrGI	TGTACA	1 cut(s)	1808
BsrI	ACTGG	5 cut(s)	394, 940, 1270, 1379, 1450
BssAI	RCCGGY	1 cut(s)	180
BssECI	CCNNGG	5 cut(s)	300, 1117, 1540, 1986, 1987
BssNI	GRCGYC	1 cut(s)	2370
BssT1I	CCWWGG	1 cut(s)	1117
Bst2UI	CCWGG	2 cut(s)	86, 512
Bst4CI	ACNGT	9 cut(s)	266, 458, 656, 677, 1100, 1127, 1157, 1327, 1865
Bst6I	CTCTTC	1 cut(s)	1376
BstACI	GRCGYC	1 cut(s)	2370
BstAUI	TGTACA	1 cut(s)	1808
BstBI	TTCGAA	2 cut(s)	97, 213
BstC8I	GCNNGC	2 cut(s)	962, 1273
BstDEI	CTNAG	8 cut(s)	336, 595, 836, 956, 1667, 1976, 2286, 2325
BstDSI	CCRYGG	1 cut(s)	1540
BstEII	GGTNACC	2 cut(s)	611, 1991
BstF5I	GGATG	5 cut(s)	484, 1255, 1367, 1455, 2132
BstFNI	CGCG	1 cut(s)	964
BstH2I	RGCGCY	1 cut(s)	1294
BstHHI	GCGC	2 cut(s)	1293, 1682
BstMAI	GTCTC	8 cut(s)	585, 959, 994, 1403, 1404, 1778, 2216, 2266
BstMWI	GCNNNNNNNGC	6 cut(s)	549, 882, 1281, 1290, 1916, 2409
BstNI	CCWGG	2 cut(s)	86, 512
BstPI	GGTNACC	2 cut(s)	611, 1991
BstSCI	CCNGG	6 cut(s)	84, 284, 300, 510, 1985, 1986
BstSFI	CTRYAG	3 cut(s)	381, 1017, 2394
BstSLI	GKGCMC	2 cut(s)	136, 947
BstUI	CGCG	1 cut(s)	964
BstV1I	GCAGC	2 cut(s)	897, 1293
BstX2I	RGATCY	2 cut(s)	1474, 2351
BstYI	RGATCY	2 cut(s)	1474, 2351
Bsu15I	ATCGAT	5 cut(s)	150, 232, 730, 2040, 2279
BsuI	GTATCC	1 cut(s)	1652
BsuRI	GGCC	3 cut(s)	134, 2310, 2412
BsuTUI	ATCGAT	5 cut(s)	150, 232, 730, 2040, 2279
BtgI	CCRYGG	1 cut(s)	1540
BtsCI	GGATG	5 cut(s)	484, 1255, 1367, 1455, 2132
BtsI	GCAGTG	2 cut(s)	1264, 2403
BtsIMutI	CAGTG	5 cut(s)	709, 947, 1264, 1861, 2403
BveI	ACCTGC	1 cut(s)	599
Cac8I	GCNNGC	2 cut(s)	962, 1273
CaiI	CAGNNNCTG	1 cut(s)	389
CfoI	GCGC	2 cut(s)	1293, 1682
Cfr10I	RCCGGY	1 cut(s)	180
Cfr13I	GGNCC	5 cut(s)	132, 133, 183, 282, 2411
Cfr9I	CCCGGG	1 cut(s)	1986
ClaI	ATCGAT	5 cut(s)	150, 232, 730, 2040, 2279
CpoI	CGGWCCG	1 cut(s)	183
CseI	GACGC	1 cut(s)	953
Csp6I	GTAC	4 cut(s)	1158, 1263, 1328, 1809
CspAI	ACCGGT	1 cut(s)	180
CspCI	CAANNNNNGTGG	6 cut(s)	658, 693, 775, 810, 1823, 1858
CspI	CGGWCCG	1 cut(s)	183
CviAII	CATG	7 cut(s)	259, 446, 714, 789, 996, 1717, 1961
CviQI	GTAC	4 cut(s)	1158, 1263, 1328, 1809
DdeI	CTNAG	8 cut(s)	336, 595, 836, 956, 1667, 1976, 2286, 2325
DraI	TTTAAA	2 cut(s)	309, 2377
Eam1104I	CTCTTC	1 cut(s)	1376
EarI	CTCTTC	1 cut(s)	1376
EciI	GGCGGA	2 cut(s)	108, 2329
Eco130I	CCWWGG	1 cut(s)	1117
Eco147I	AGGCCT	1 cut(s)	2310
Eco24I	GRGCYC	3 cut(s)	136, 1047, 1217
Eco31I	GGTCTC	2 cut(s)	959, 2266
Eco32I	GATATC	1 cut(s)	2176
Eco47I	GGWCC	2 cut(s)	183, 282
Eco47III	AGCGCT	1 cut(s)	1292
Eco57I	CTGAAG	5 cut(s)	675, 840, 933, 1641, 2189
Eco88I	CYCGRG	1 cut(s)	1986
Eco91I	GGTNACC	2 cut(s)	611, 1991
EcoO109I	RGGNCCY	1 cut(s)	132
EcoO65I	GGTNACC	2 cut(s)	611, 1991
EcoRI	GAATTC	1 cut(s)	188
EcoRII	CCWGG	2 cut(s)	84, 510
EcoRV	GATATC	1 cut(s)	2176
EcoT14I	CCWWGG	1 cut(s)	1117
EcoT38I	GRGCYC	3 cut(s)	136, 1047, 1217
ErhI	CCWWGG	1 cut(s)	1117
FaeI	CATG	7 cut(s)	262, 449, 717, 792, 999, 1720, 1964
FalI	AAGNNNNNCTT	4 cut(s)	1277, 1309, 2298, 2330
FaqI	GGGAC	4 cut(s)	207, 1052, 1573, 1614
FatI	CATG	7 cut(s)	258, 445, 713, 788, 995, 1716, 1960
FauI	CCCGC	2 cut(s)	152, 973
FbaI	TGATCA	1 cut(s)	1308
Fnu4HI	GCNGC	3 cut(s)	886, 1282, 2343
FokI	GGATG	5 cut(s)	471, 1262, 1374, 1442, 2119
FriOI	GRGCYC	3 cut(s)	136, 1047, 1217
Fsp4HI	GCNGC	3 cut(s)	886, 1282, 2343
FspBI	CTAG	7 cut(s)	219, 549, 1511, 1823, 1892, 1947, 2069
FspI	TGCGCA	1 cut(s)	1681
GlaI	GCGC	2 cut(s)	1292, 1681
GluI	GCNGC	3 cut(s)	886, 1282, 2343
GsaI	CCCAGC	1 cut(s)	1038
GsuI	CTGGAG	1 cut(s)	609
HaeII	RGCGCY	1 cut(s)	1294
HaeIII	GGCC	3 cut(s)	134, 2310, 2412
HapII	CCGG	5 cut(s)	181, 285, 302, 1987, 2101
HgaI	GACGC	1 cut(s)	953
HhaI	GCGC	2 cut(s)	1293, 1682
Hin1I	GRCGYC	1 cut(s)	2370
Hin1II	CATG	7 cut(s)	262, 449, 717, 792, 999, 1720, 1964
Hin6I	GCGC	2 cut(s)	1291, 1680
HinP1I	GCGC	2 cut(s)	1291, 1680
HpaII	CCGG	5 cut(s)	181, 285, 302, 1987, 2101
HphI	GGTGA	2 cut(s)	461, 1516
Hpy166II	GTNNAC	4 cut(s)	256, 1537, 1809, 1861
Hpy188I	TCNGA	8 cut(s)	76, 187, 316, 1207, 1417, 2032, 2116, 2383
Hpy188III	TCNNGA	9 cut(s)	280, 429, 588, 858, 1243, 1321, 1733, 2146, 2296
Hpy8I	GTNNAC	4 cut(s)	256, 1537, 1809, 1861
Hpy99I	CGWCG	2 cut(s)	365, 969
HpyCH4III	ACNGT	9 cut(s)	266, 458, 656, 677, 1100, 1127, 1157, 1327, 1865
HpyCH4IV	ACGT	2 cut(s)	1109, 2370
HpyF10VI	GCNNNNNNNGC	6 cut(s)	549, 882, 1281, 1290, 1916, 2409
HpyF3I	CTNAG	8 cut(s)	336, 595, 836, 956, 1667, 1976, 2286, 2325
HpySE526I	ACGT	2 cut(s)	1109, 2370
Hsp92I	GRCGYC	1 cut(s)	2370
Hsp92II	CATG	7 cut(s)	262, 449, 717, 792, 999, 1720, 1964
HspAI	GCGC	2 cut(s)	1291, 1680
Ksp22I	TGATCA	1 cut(s)	1308
LmnI	GCTCC	1 cut(s)	557
Lsp1109I	GCAGC	2 cut(s)	897, 1293
LweI	GCATC	4 cut(s)	322, 1352, 1599, 2289
MaeI	CTAG	7 cut(s)	219, 549, 1511, 1823, 1892, 1947, 2069
MaeII	ACGT	2 cut(s)	1109, 2370
MaeIII	GTNAC	7 cut(s)	611, 685, 926, 1466, 1624, 1865, 1991
MfeI	CAATTG	1 cut(s)	846
MflI	RGATCY	2 cut(s)	1474, 2351
MhlI	GDGCHC	4 cut(s)	136, 947, 1047, 1217
MlyI	GAGTC	3 cut(s)	419, 1406, 1555
MmeI	TCCRAC	5 cut(s)	512, 579, 1563, 1755, 2357
MroXI	GAANNNNTTC	3 cut(s)	32, 1292, 1884
MseI	TTAA	4 cut(s)	114, 308, 1236, 2376
MslI	CAYNNNNRTG	3 cut(s)	450, 2005, 2245
MspI	CCGG	5 cut(s)	181, 285, 302, 1987, 2101
MspR9I	CCNGG	6 cut(s)	86, 286, 302, 512, 1987, 1988
MunI	CAATTG	1 cut(s)	846
Mva1269I	GAATGC	1 cut(s)	2064
MvaI	CCWGG	2 cut(s)	86, 512
MvnI	CGCG	1 cut(s)	964
MwoI	GCNNNNNNNGC	6 cut(s)	549, 882, 1281, 1290, 1916, 2409
NciI	CCSGG	4 cut(s)	286, 302, 1987, 1988
NlaIII	CATG	7 cut(s)	262, 449, 717, 792, 999, 1720, 1964
NlaIV	GGNNCC	5 cut(s)	133, 134, 298, 509, 1984
NmuCI	GTSAC	2 cut(s)	685, 1865
NsbI	TGCGCA	1 cut(s)	1681
NspV	TTCGAA	2 cut(s)	97, 213
OliI	CACNNNNGTG	2 cut(s)	2005, 2245
PceI	AGGCCT	1 cut(s)	2310
PcsI	WCGNNNNNNNCGW	1 cut(s)	1842
PctI	GAATGC	1 cut(s)	2064
PdmI	GAANNNNTTC	3 cut(s)	32, 1292, 1884
PfeI	GAWTC	7 cut(s)	210, 776, 878, 905, 1546, 1766, 1847
PinAI	ACCGGT	1 cut(s)	180
PkrI	GCNGC	3 cut(s)	887, 1283, 2344
PleI	GAGTC	3 cut(s)	419, 1405, 1555
PpsI	GAGTC	3 cut(s)	419, 1405, 1555
PshBI	ATTAAT	1 cut(s)	114
Psp6I	CCWGG	2 cut(s)	84, 510
PspEI	GGTNACC	2 cut(s)	611, 1991
PspFI	CCCAGC	1 cut(s)	1034
PspGI	CCWGG	2 cut(s)	84, 510
PspN4I	GGNNCC	5 cut(s)	133, 134, 298, 509, 1984
PspOMI	GGGCCC	1 cut(s)	132
PspPI	GGNCC	5 cut(s)	132, 133, 183, 282, 2411
PstI	CTGCAG	2 cut(s)	385, 2398
PstNI	CAGNNNCTG	1 cut(s)	389
PsuI	RGATCY	2 cut(s)	1474, 2351
RsaI	GTAC	4 cut(s)	1159, 1264, 1329, 1810
RsaNI	GTAC	4 cut(s)	1158, 1263, 1328, 1809
RseI	CAYNNNNRTG	3 cut(s)	450, 2005, 2245
Rsr2I	CGGWCCG	1 cut(s)	183
RsrII	CGGWCCG	1 cut(s)	183
SaqAI	TTAA	4 cut(s)	114, 308, 1236, 2376
SatI	GCNGC	3 cut(s)	886, 1282, 2343
Sau96I	GGNCC	5 cut(s)	132, 133, 183, 282, 2411
ScaI	AGTACT	1 cut(s)	1329
SchI	GAGTC	3 cut(s)	419, 1406, 1555
ScrFI	CCNGG	6 cut(s)	86, 286, 302, 512, 1987, 1988
SduI	GDGCHC	4 cut(s)	136, 947, 1047, 1217
SfaNI	GCATC	4 cut(s)	322, 1352, 1599, 2289
SfcI	CTRYAG	3 cut(s)	381, 1017, 2394
SfuI	TTCGAA	2 cut(s)	97, 213
SinI	GGWCC	2 cut(s)	183, 282
SmaI	CCCGGG	1 cut(s)	1988
SmiMI	CAYNNNNRTG	3 cut(s)	450, 2005, 2245
SmlI	CTYRAG	4 cut(s)	562, 763, 1243, 2269
SmoI	CTYRAG	4 cut(s)	562, 763, 1243, 2269
SpeI	ACTAGT	1 cut(s)	218
SseBI	AGGCCT	1 cut(s)	2310
SsiI	CCGC	5 cut(s)	119, 159, 980, 2340, 2343
SspI	AATATT	1 cut(s)	772
SspMI	CTAG	7 cut(s)	219, 549, 1511, 1823, 1892, 1947, 2069
StuI	AGGCCT	1 cut(s)	2310
StyD4I	CCNGG	6 cut(s)	84, 284, 300, 510, 1985, 1986
StyI	CCWWGG	1 cut(s)	1117
TaaI	ACNGT	9 cut(s)	266, 458, 656, 677, 1100, 1127, 1157, 1327, 1865
TaiI	ACGT	2 cut(s)	1112, 2373
TaqII	GACCGA	1 cut(s)	1583
TatI	WGTACW	3 cut(s)	1157, 1327, 1808
TauI	GCSGC	1 cut(s)	2345
TfiI	GAWTC	7 cut(s)	210, 776, 878, 905, 1546, 1766, 1847
Tru1I	TTAA	4 cut(s)	114, 308, 1236, 2376
Tru9I	TTAA	4 cut(s)	114, 308, 1236, 2376
TscAI	CASTG	5 cut(s)	709, 947, 1264, 1868, 2403
TseFI	GTSAC	2 cut(s)	685, 1865
TseI	GCWGC	2 cut(s)	885, 1281
Tsp45I	GTSAC	2 cut(s)	685, 1865
TspDTI	ATGAA	6 cut(s)	7, 11, 34, 545, 702, 897
TspGWI	ACGGA	4 cut(s)	378, 536, 1421, 1448
TspMI	CCCGGG	1 cut(s)	1986
TspRI	CASTG	5 cut(s)	709, 947, 1264, 1868, 2403
VpaK11BI	GGWCC	2 cut(s)	183, 282
VspI	ATTAAT	1 cut(s)	114
XcmI	CCANNNNNNNNNTGG	1 cut(s)	630
XmaI	CCCGGG	1 cut(s)	1986
XmnI	GAANNNNTTC	3 cut(s)	32, 1292, 1884
XspI	CTAG	7 cut(s)	219, 549, 1511, 1823, 1892, 1947, 2069
ZraI	GACGTC	1 cut(s)	2371
ZrmI	AGTACT	1 cut(s)	1329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.