Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0002245

Physical Location & Seq

Genomic Coordinates Reverse (-)

7634359 .. 7636557

Gene Structure 2199 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g11300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGGACAGTTCTACGGTAGTCTGGACTGCGAATAGGGGTTCCCCGGTTTCAAATTCTGATAAAATTGTGTTTGATGCTAAGAGTGGTGCGTCTTTGCAAAAAGGTGGAAGTGTGGTTTGGTCTGCGGATACTGGTGGTAAAAGCGTATCTGCAATGGAATTGCAGGACTCGGGAAATTTGGTTTTGCGTGGAGATGACAATGGAGTAGTTTGGCAGAGTTTTAGGCATCCAACTGATACCCTATTATGGAATCAGGAATTTTCAGAAGGAATGAAGCTTGTAAGCAATCCTAGCTCTAACAACTTGACTTATATTCTTCAAATCAAGTCCGGGGACTTGGTTCTTTCTGCAGGTTTCCAAACACCACAGCCGTATTGGTCTATGGCGAAAGAAAGCCGTAAAACAGTCAACATAGATGGTGGTGTAGTCACTTCAGCAACTATCAGTGAAAATTCATGGAAGTTCTTTGATCGATCCAAAGCGTTGCTATGGCAATTTATTTTCTCGAGCAATATTGATGCAAATGCCACATGGATTGCAGTTTTAGGAAGTGATGGATTTATCTCTTTCCACAATCTTCAGAATGGAGGGTCAAGTGGTCCTTCAGAAACAAAAATACCAAGCGATTCGTGCAGCACACCAGAGCCTTGTGATTCATACTTCGAATGTTATAGTAACAACAAGTGCCAGTGCCCTTCAGGTCTCAGCTCGCGTACTAATTGCAAGGCGGGGGTTGTCTCCTCTTGTGATCCTTCGGAGGGTTCTACAGAGCTTGTAAATGCTGGGGACGGGCTCTATTATTTTGCATTGGGATATATTCCACCTTCTTCGAAAACTGATTTGAATGGTTGCCAAACGTCTTGCCTTGGCAACTGTTCGTGTGTTGCTATGTTCTTCCAAAACAGTACAAGAAATTGTTTTCTGTTTGACAGATTAGGTAGCTTCCAAAATTCTGACAAGGGCTCTGGCTTTGTTTCTTACATTAAGGTCTTGAGGGATGGAAGCAGTGGGAGGAGCGGAAACAACAAGAAACACTTCCCTTATGTTGTGATCATAGCCATCTCGACAGTACTTGTTATTTGTGGCCTACTTTTTATGGGATACCGGTACTACAGGAGGAAGGGAAGATCACCAGAATCTCCTACAGGGACCTCAGACGAGGAAAATTTCTTTGAAAATATGACTGGGATGCCCATTCGTTTCAGTTACAGAGATCTCCAAACTGCAACAAATAACTACTCGGTGAAGCTAGGGCAAGGAGGTTTTGGCTCGGTTTACCAAGGGAGTCTCCCAGATGGGACTCAACTGGCTGTGAAGAAGTTGGAAGGCATTGGTCAGGGAAAGAAAGAGTTTCGAGCTGAAGTTAGCATCATTGGCAGCATCCATCATCTGCACTTGGTCCGGCTCAGGGGCTTCTGTGCAGAAGGAAGTCATCGACTTCTGGTTTACGATTACATGGCAAATGGGTCTCTTGATAAATGGATTTTCAAGAAAAACAACGGAGAATTCCTATTGGATTGGGAGACAAGATTCAATATAGCATTGGGTACGGCTAAAGGGCTAGCATATCTCCACGAAGATTGTGATGTGAAGATTGTTCACTGTGACATAAAGCCTGAAAATGTTCTCCTCGACACTAATTACCTTGCCAAAGTCTCAGATTTTGGTTTGGCAAAGCTAATGACCAGGGAACAGAGCCATGTTTTCACAACGCTAAGAGGAACCCGAGGGTACCTTGCACCGGAGTGGATCACCAACTACGCAATATCAGAGAAGAGTGATGTGTACAGCTATGGAATGCTCTTGCTAGAGATCATTGGTGGGAGAAAGAATTACGACCCGACAGAAACTTCAGAAAAATCCCATCTTCCATCTTATGCCTTCAAGATGTTGGAAGAAGGCAAAATGAGGGACATCCTGGACACAATGCTAGGGAAAGATGAAGCCGACGAGAGGGTACACACAGCCATTATGGTTGCACTGTGGTGCATACAGGAGGATATGTCTGTGAGACCATCAATGACGAAGGTTGTCCAAATGCTTGAAGGCCTGTATCCCGTTCCCCAGCCTCCAACCTCCAACTCAATGGGGTCTCGCCTTTATCCGAATTTCTTCAGATCAGTCAGCGGGGAGGGGACTTCCTCAGGACCATCAGACTGCAACAGCGTTTCTGCAGTGCGCCTCTCCGGCCCAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0031625 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044464 GO:0046777 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr

Protein Analysis

733

Amino Acids

80.46

Weight (kDa)

6.35

Isoelectric Point (pI)

38.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	5 - 87	4e-16	D-mannose binding lectin
Pkinase	PF00069	415 - 679	5.6e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	415 - 681	3.7e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002245)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32300
fragaria_vesca	FvH4_2g29050 FvH4_2g29070
malus_domestica	MD00G1144200.v1.1 MD15G1121900.v1.1 MD15G1122800.v1.1 MD15G1125500.v1.1
prunus_persica	Prupe.1G481100_v2.0.a1
pyrus_communis	pycom15g10990 pycom15g11300
rosa_chinensis	RchiOBHm_Chr4g0385931 RchiOBHm_Chr6g0297921 RchiOBHm_Chr6g0297931
rosa_laevigata	RLG00000010199 RLG00000011550 RLG00000011551
rosa_multiflora	Rmu_co8186410.1_g000001 Rmu_sc0007302.1_g000003 Rmu_sc0007302.1_g000004 Rmu_sc0007400.1_g000011 Rmu_sc0011097.1_g000002 Rmu_sc0011097.1_g000003 Rmu_sc0011097.1_g000004 Rmu_sc0015868.1_g000001 Rmu_sc0030316.1_g000001
rosa_roxburghii	Rroxscaffold_5G00333530 Rroxscaffold_7G00170060 Rroxscaffold_7G00178900 Rroxscaffold_7G00178910
rosa_rugosa	Rorug03G0303100 Rorug06G0276800
rosa_samantha	Rh4CG008200 Rh4DG007100 Rh6AG387500 Rh6BG395900 Rh6CG401600 Rh6CG401700 Rh6CG401800 Rh6DG388100
rosa_wichuraiana	Rw4G000320 Rw6G033760 Rw6G033810 Rw6G033820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	341
Acc65I	GGTACC	1 cut(s)	1731
AccB1I	GGYRCC	1 cut(s)	1731
AccBSI	CCGCTC	1 cut(s)	1017
AccII	CGCG	1 cut(s)	712
AciI	CCGC	4 cut(s)	125, 728, 1017, 2127
AclWI	GGATC	3 cut(s)	468, 743, 1757
AcsI	RAATTY	8 cut(s)	52, 175, 257, 451, 949, 1165, 1505, 2107
AcuI	CTGAAG	7 cut(s)	417, 563, 588, 681, 1380, 1836, 2098
AfaI	GTAC	8 cut(s)	715, 907, 1071, 1109, 1549, 1733, 1787, 1959
AfiI	CCNNNNNNNGG	5 cut(s)	246, 758, 1408, 1741, 1953
AgeI	ACCGGT	1 cut(s)	1104
AgsI	TTSAA	8 cut(s)	51, 320, 844, 1175, 1489, 1534, 1885, 2045
AjnI	CCWGG	2 cut(s)	1685, 1917
AjuI	GAANNNNNNNTTGG	2 cut(s)	100, 132
AleI	CACNNNNGTG	2 cut(s)	1744, 1984
AluBI	AGCT	9 cut(s)	277, 294, 708, 772, 942, 1249, 1358, 1678, 1791
AluI	AGCT	9 cut(s)	277, 294, 708, 772, 942, 1249, 1358, 1678, 1791
Alw26I	GTCTC	8 cut(s)	707, 742, 1292, 1473, 1517, 1660, 2005, 2097
AlwI	GGATC	3 cut(s)	468, 743, 1757
Ama87I	CYCGRG	3 cut(s)	169, 505, 1725
AoxI	GGCC	3 cut(s)	1084, 2047, 2188
ApeKI	GCWGC	2 cut(s)	633, 1377
ApoI	RAATTY	8 cut(s)	52, 175, 257, 451, 949, 1165, 1505, 2107
AsiGI	ACCGGT	1 cut(s)	1104
Asp700I	GAANNNNTTC	3 cut(s)	1034, 1623, 2111
Asp718I	GGTACC	1 cut(s)	1731
AspLEI	GCGC	1 cut(s)	2181
AspS9I	GGNCC	5 cut(s)	599, 1149, 1399, 2147, 2189
AsuC2I	CCSGG	2 cut(s)	44, 331
AsuHPI	GGTGA	3 cut(s)	1122, 1255, 1744
AsuII	TTCGAA	2 cut(s)	663, 830
AsuNHI	GCTAGC	1 cut(s)	1561
AvaI	CYCGRG	3 cut(s)	169, 505, 1725
AvaII	GGWCC	4 cut(s)	599, 1149, 1399, 2147
AxyI	CCTNAGG	1 cut(s)	2143
BaeGI	GKGCMC	1 cut(s)	695
BanI	GGYRCC	1 cut(s)	1731
BanII	GRGCYC	2 cut(s)	795, 965
BbvI	GCAGC	2 cut(s)	645, 1389
BceAI	ACGGC	3 cut(s)	355, 381, 1566
BciT130I	CCWGG	2 cut(s)	1687, 1919
BciVI	GTATCC	3 cut(s)	121, 1094, 2064
BclI	TGATCA	1 cut(s)	1050
BcnI	CCSGG	2 cut(s)	44, 331
BcoDI	GTCTC	8 cut(s)	707, 742, 1292, 1473, 1517, 1660, 2005, 2097
BfaI	CTAG	5 cut(s)	291, 1250, 1562, 1808, 1931
BfmI	CTRYAG	5 cut(s)	348, 765, 1111, 1143, 2172
BfuAI	ACCTGC	1 cut(s)	341
BfuI	GTATCC	3 cut(s)	121, 1094, 2064
BglI	GCCNNNNNGGC	1 cut(s)	2187
BglII	AGATCT	1 cut(s)	1213
BisI	GCNGC	2 cut(s)	634, 1378
BlsI	GCNGC	2 cut(s)	635, 1379
BmcAI	AGTACT	1 cut(s)	1071
Bme1390I	CCNGG	4 cut(s)	44, 331, 1687, 1919
Bme18I	GGWCC	4 cut(s)	599, 1149, 1399, 2147
BmeT110I	CYCGRG	3 cut(s)	169, 505, 1725
BmgT120I	GGNCC	5 cut(s)	599, 1149, 1399, 2147, 2189
BmiI	GGNNCC	4 cut(s)	40, 1150, 1723, 1733
BmrFI	CCNGG	4 cut(s)	44, 331, 1687, 1919
BmrI	ACTGGG	1 cut(s)	1194
BmsI	GCATC	6 cut(s)	64, 235, 508, 1179, 1377, 1389
BmtI	GCTAGC	1 cut(s)	1565
BmuI	ACTGGG	1 cut(s)	1194
Bpu10I	CCTNAGC	1 cut(s)	1406
Bpu14I	TTCGAA	2 cut(s)	663, 830
BpuEI	CTTGAG	1 cut(s)	1012
BpuMI	CCSGG	2 cut(s)	44, 331
Bsa29I	ATCGAT	1 cut(s)	472
BsaI	GGTCTC	4 cut(s)	707, 1473, 2005, 2097
BsaJI	CCNNGG	6 cut(s)	42, 330, 865, 1279, 1686, 1726
BsaWI	WCCGGW	2 cut(s)	1104, 1741
BsaXI	ACNNNNNCTCC	2 cut(s)	1988, 2018
Bsc4I	CCNNNNNNNGG	5 cut(s)	246, 758, 1408, 1741, 1953
Bse118I	RCCGGY	1 cut(s)	1104
Bse1I	ACTGG	4 cut(s)	136, 688, 1189, 1311
Bse21I	CCTNAGG	1 cut(s)	2143
Bse3DI	GCAATG	1 cut(s)	159
BseBI	CCWGG	2 cut(s)	1687, 1919
BseCI	ATCGAT	1 cut(s)	472
BseDI	CCNNGG	6 cut(s)	42, 330, 865, 1279, 1686, 1726
BseGI	GGATG	5 cut(s)	226, 1003, 1194, 1380, 1914
BseLI	CCNNNNNNNGG	5 cut(s)	246, 758, 1408, 1741, 1953
BseMI	GCAATG	1 cut(s)	159
BseMII	CTCAG	5 cut(s)	718, 1167, 1420, 1671, 2157
BseNI	ACTGG	4 cut(s)	136, 688, 1189, 1311
BseRI	GAGGAG	3 cut(s)	730, 1027, 1619
BseSI	GKGCMC	1 cut(s)	695
BseXI	GCAGC	2 cut(s)	645, 1389
BseYI	CCCAGC	2 cut(s)	782, 2064
BsgI	GTGCAG	3 cut(s)	652, 1376, 1440
Bsh1236I	CGCG	1 cut(s)	712
BshFI	GGCC	3 cut(s)	1086, 2049, 2190
BshNI	GGYRCC	1 cut(s)	1731
BshTI	ACCGGT	1 cut(s)	1104
BshVI	ATCGAT	1 cut(s)	472
BsiHKCI	CYCGRG	3 cut(s)	169, 505, 1725
BsiSI	CCGG	6 cut(s)	44, 330, 1105, 1402, 1742, 2187
BslFI	GGGAC	6 cut(s)	347, 800, 1162, 1312, 1925, 2149
BslI	CCNNNNNNNGG	5 cut(s)	246, 758, 1408, 1741, 1953
BsmAI	GTCTC	8 cut(s)	707, 742, 1292, 1473, 1517, 1660, 2005, 2097
BsmFI	GGGAC	6 cut(s)	347, 800, 1162, 1312, 1925, 2149
BsmI	GAATGC	1 cut(s)	1803
BsnI	GGCC	3 cut(s)	1086, 2049, 2190
Bso31I	GGTCTC	4 cut(s)	707, 1473, 2005, 2097
BsoBI	CYCGRG	3 cut(s)	169, 505, 1725
Bsp119I	TTCGAA	2 cut(s)	663, 830
Bsp1286I	GDGCHC	3 cut(s)	695, 795, 965
Bsp1407I	TGTACA	1 cut(s)	1785
Bsp143I	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
BspACI	CCGC	4 cut(s)	125, 728, 1017, 2127
BspANI	GGCC	3 cut(s)	1086, 2049, 2190
BspCNI	CTCAG	5 cut(s)	717, 1166, 1419, 1670, 2156
BspDI	ATCGAT	1 cut(s)	472
BspFNI	CGCG	1 cut(s)	712
BspLI	GGNNCC	4 cut(s)	40, 1150, 1723, 1733
BspMAI	CTGCAG	2 cut(s)	352, 2176
BspMI	ACCTGC	1 cut(s)	341
BspOI	GCTAGC	1 cut(s)	1565
BspPI	GGATC	3 cut(s)	468, 743, 1757
BspT104I	TTCGAA	2 cut(s)	663, 830
BspT107I	GGYRCC	1 cut(s)	1731
BspTNI	GGTCTC	4 cut(s)	707, 1473, 2005, 2097
BsrBI	CCGCTC	1 cut(s)	1017
BsrDI	GCAATG	1 cut(s)	159
BsrFI	RCCGGY	1 cut(s)	1104
BsrGI	TGTACA	1 cut(s)	1785
BsrI	ACTGG	4 cut(s)	136, 688, 1189, 1311
BssAI	RCCGGY	1 cut(s)	1104
BssECI	CCNNGG	6 cut(s)	42, 330, 865, 1279, 1686, 1726
BssMI	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
BssT1I	CCWWGG	2 cut(s)	865, 1279
Bst2UI	CCWGG	2 cut(s)	1687, 1919
Bst4CI	ACNGT	8 cut(s)	8, 16, 406, 875, 905, 1069, 1604, 1983
Bst6I	CTCTTC	1 cut(s)	1769
BstAUI	TGTACA	1 cut(s)	1785
BstBI	TTCGAA	2 cut(s)	663, 830
BstC8I	GCNNGC	2 cut(s)	710, 1563
BstDEI	CTNAG	7 cut(s)	78, 704, 1153, 1406, 1657, 1715, 2143
BstF5I	GGATG	5 cut(s)	226, 1003, 1194, 1380, 1914
BstFNI	CGCG	1 cut(s)	712
BstHHI	GCGC	1 cut(s)	2181
BstKTI	GATC	9 cut(s)	472, 476, 751, 1053, 1130, 1216, 1752, 1815, 2120
BstMAI	GTCTC	8 cut(s)	707, 742, 1292, 1473, 1517, 1660, 2005, 2097
BstMBI	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
BstMWI	GCNNNNNNNGC	4 cut(s)	291, 630, 1374, 2187
BstNI	CCWGG	2 cut(s)	1687, 1919
BstSCI	CCNGG	4 cut(s)	42, 329, 1685, 1917
BstSFI	CTRYAG	5 cut(s)	348, 765, 1111, 1143, 2172
BstSLI	GKGCMC	1 cut(s)	695
BstUI	CGCG	1 cut(s)	712
BstV1I	GCAGC	2 cut(s)	645, 1389
BstX2I	RGATCY	1 cut(s)	1213
BstXI	CCANNNNNNTGG	1 cut(s)	2086
BstYI	RGATCY	1 cut(s)	1213
Bsu15I	ATCGAT	1 cut(s)	472
Bsu36I	CCTNAGG	1 cut(s)	2143
BsuI	GTATCC	3 cut(s)	121, 1094, 2064
BsuRI	GGCC	3 cut(s)	1086, 2049, 2190
BsuTUI	ATCGAT	1 cut(s)	472
BtsCI	GGATG	5 cut(s)	226, 1003, 1194, 1380, 1914
BtsI	GCAGTG	2 cut(s)	1012, 2181
BtsIMutI	CAGTG	6 cut(s)	451, 695, 1012, 1600, 1979, 2181
BveI	ACCTGC	1 cut(s)	341
Cac8I	GCNNGC	2 cut(s)	710, 1563
CfoI	GCGC	1 cut(s)	2181
Cfr10I	RCCGGY	1 cut(s)	1104
Cfr13I	GGNCC	5 cut(s)	599, 1149, 1399, 2147, 2189
ClaI	ATCGAT	1 cut(s)	472
CseI	GACGC	1 cut(s)	78
Csp6I	GTAC	8 cut(s)	714, 906, 1070, 1108, 1548, 1732, 1786, 1958
CspAI	ACCGGT	1 cut(s)	1104
CspCI	CAANNNNNGTGG	4 cut(s)	517, 552, 1562, 1597
CviAII	CATG	4 cut(s)	456, 531, 1456, 1700
CviQI	GTAC	8 cut(s)	714, 906, 1070, 1108, 1548, 1732, 1786, 1958
DdeI	CTNAG	7 cut(s)	78, 704, 1153, 1406, 1657, 1715, 2143
DpnI	GATC	9 cut(s)	471, 475, 750, 1052, 1129, 1215, 1751, 1814, 2119
DpnII	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
Eam1104I	CTCTTC	1 cut(s)	1769
EarI	CTCTTC	1 cut(s)	1769
Eco130I	CCWWGG	2 cut(s)	865, 1279
Eco147I	AGGCCT	1 cut(s)	2049
Eco24I	GRGCYC	2 cut(s)	795, 965
Eco31I	GGTCTC	4 cut(s)	707, 1473, 2005, 2097
Eco47I	GGWCC	4 cut(s)	599, 1149, 1399, 2147
Eco57I	CTGAAG	7 cut(s)	417, 563, 588, 681, 1380, 1836, 2098
Eco81I	CCTNAGG	1 cut(s)	2143
Eco88I	CYCGRG	3 cut(s)	169, 505, 1725
EcoO109I	RGGNCCY	1 cut(s)	1149
EcoRI	GAATTC	1 cut(s)	1505
EcoRII	CCWGG	2 cut(s)	1685, 1917
EcoT14I	CCWWGG	2 cut(s)	865, 1279
EcoT38I	GRGCYC	2 cut(s)	795, 965
ErhI	CCWWGG	2 cut(s)	865, 1279
FaeI	CATG	4 cut(s)	459, 534, 1459, 1703
FalI	AAGNNNNNCTT	4 cut(s)	586, 618, 1019, 1051
FaqI	GGGAC	6 cut(s)	347, 800, 1162, 1312, 1925, 2149
FatI	CATG	4 cut(s)	455, 530, 1455, 1699
FauI	CCCGC	2 cut(s)	721, 2120
FbaI	TGATCA	1 cut(s)	1050
Fnu4HI	GCNGC	2 cut(s)	634, 1378
FokI	GGATG	5 cut(s)	213, 1010, 1201, 1367, 1901
FriOI	GRGCYC	2 cut(s)	795, 965
Fsp4HI	GCNGC	2 cut(s)	634, 1378
FspBI	CTAG	5 cut(s)	291, 1250, 1562, 1808, 1931
GlaI	GCGC	1 cut(s)	2180
GluI	GCNGC	2 cut(s)	634, 1378
GsaI	CCCAGC	2 cut(s)	786, 2068
HaeIII	GGCC	3 cut(s)	1086, 2049, 2190
HapII	CCGG	6 cut(s)	44, 330, 1105, 1402, 1742, 2187
HgaI	GACGC	1 cut(s)	78
HhaI	GCGC	1 cut(s)	2181
Hin1II	CATG	4 cut(s)	459, 534, 1459, 1703
Hin6I	GCGC	1 cut(s)	2179
HinP1I	GCGC	1 cut(s)	2179
HincII	GTYRAC	1 cut(s)	409
HindII	GTYRAC	1 cut(s)	409
HindIII	AAGCTT	1 cut(s)	275
HinfI	GANTC	8 cut(s)	167, 250, 626, 653, 1136, 1285, 1300, 1530
HpaII	CCGG	6 cut(s)	44, 330, 1105, 1402, 1742, 2187
HphI	GGTGA	3 cut(s)	1122, 1255, 1744
Hpy166II	GTNNAC	6 cut(s)	409, 1276, 1447, 1600, 1786, 1960
Hpy8I	GTNNAC	6 cut(s)	409, 1276, 1447, 1600, 1786, 1960
Hpy99I	CGWCG	1 cut(s)	1952
HpyCH4III	ACNGT	8 cut(s)	8, 16, 406, 875, 905, 1069, 1604, 1983
HpyCH4IV	ACGT	1 cut(s)	857
HpyF10VI	GCNNNNNNNGC	4 cut(s)	291, 630, 1374, 2187
HpyF3I	CTNAG	7 cut(s)	78, 704, 1153, 1406, 1657, 1715, 2143
HpySE526I	ACGT	1 cut(s)	857
Hsp92II	CATG	4 cut(s)	459, 534, 1459, 1703
HspAI	GCGC	1 cut(s)	2179
KpnI	GGTACC	1 cut(s)	1735
Ksp22I	TGATCA	1 cut(s)	1050
Kzo9I	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
LmnI	GCTCC	1 cut(s)	1014
Lsp1109I	GCAGC	2 cut(s)	645, 1389
LweI	GCATC	6 cut(s)	64, 235, 508, 1179, 1377, 1389
MaeI	CTAG	5 cut(s)	291, 1250, 1562, 1808, 1931
MaeII	ACGT	1 cut(s)	857
MaeIII	GTNAC	4 cut(s)	427, 674, 1205, 1604
MalI	GATC	9 cut(s)	471, 475, 750, 1052, 1129, 1215, 1751, 1814, 2119
MbiI	CCGCTC	1 cut(s)	1017
MboI	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
MflI	RGATCY	1 cut(s)	1213
MhlI	GDGCHC	3 cut(s)	695, 795, 965
MlyI	GAGTC	3 cut(s)	161, 1294, 1294
MmeI	TCCRAC	5 cut(s)	254, 1302, 1872, 2096, 2103
MroXI	GAANNNNTTC	3 cut(s)	1034, 1623, 2111
MseI	TTAA	1 cut(s)	984
MslI	CAYNNNNRTG	2 cut(s)	1744, 1984
MspA1I	CMGCKG	1 cut(s)	2127
MspI	CCGG	6 cut(s)	44, 330, 1105, 1402, 1742, 2187
MspR9I	CCNGG	4 cut(s)	44, 331, 1687, 1919
Mva1269I	GAATGC	1 cut(s)	1803
MvaI	CCWGG	2 cut(s)	1687, 1919
MvnI	CGCG	1 cut(s)	712
MwoI	GCNNNNNNNGC	4 cut(s)	291, 630, 1374, 2187
NciI	CCSGG	2 cut(s)	44, 331
NdeII	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
NheI	GCTAGC	1 cut(s)	1561
NlaIII	CATG	4 cut(s)	459, 534, 1459, 1703
NlaIV	GGNNCC	4 cut(s)	40, 1150, 1723, 1733
NmuCI	GTSAC	2 cut(s)	427, 1604
NspV	TTCGAA	2 cut(s)	663, 830
OliI	CACNNNNGTG	2 cut(s)	1744, 1984
PaeR7I	CTCGAG	1 cut(s)	505
PceI	AGGCCT	1 cut(s)	2049
PctI	GAATGC	1 cut(s)	1803
PdmI	GAANNNNTTC	3 cut(s)	1034, 1623, 2111
PfeI	GAWTC	5 cut(s)	250, 626, 653, 1136, 1530
PfoI	TCCNGGA	1 cut(s)	1917
PinAI	ACCGGT	1 cut(s)	1104
PkrI	GCNGC	2 cut(s)	635, 1379
PleI	GAGTC	3 cut(s)	161, 1293, 1294
PpsI	GAGTC	3 cut(s)	161, 1293, 1294
PpuMI	RGGWCCY	1 cut(s)	1149
Psp5II	RGGWCCY	1 cut(s)	1149
Psp6I	CCWGG	2 cut(s)	1685, 1917
PspFI	CCCAGC	2 cut(s)	782, 2064
PspGI	CCWGG	2 cut(s)	1685, 1917
PspN4I	GGNNCC	4 cut(s)	40, 1150, 1723, 1733
PspPI	GGNCC	5 cut(s)	599, 1149, 1399, 2147, 2189
PspPPI	RGGWCCY	1 cut(s)	1149
PstI	CTGCAG	2 cut(s)	352, 2176
PsuI	RGATCY	1 cut(s)	1213
RsaI	GTAC	8 cut(s)	715, 907, 1071, 1109, 1549, 1733, 1787, 1959
RsaNI	GTAC	8 cut(s)	714, 906, 1070, 1108, 1548, 1732, 1786, 1958
RseI	CAYNNNNRTG	2 cut(s)	1744, 1984
SaqAI	TTAA	1 cut(s)	984
SatI	GCNGC	2 cut(s)	634, 1378
Sau3AI	GATC	9 cut(s)	469, 473, 748, 1050, 1127, 1213, 1749, 1812, 2117
Sau96I	GGNCC	5 cut(s)	599, 1149, 1399, 2147, 2189
ScaI	AGTACT	1 cut(s)	1071
SchI	GAGTC	3 cut(s)	161, 1294, 1294
ScrFI	CCNGG	4 cut(s)	44, 331, 1687, 1919
SduI	GDGCHC	3 cut(s)	695, 795, 965
SfaNI	GCATC	6 cut(s)	64, 235, 508, 1179, 1377, 1389
SfcI	CTRYAG	5 cut(s)	348, 765, 1111, 1143, 2172
Sfr274I	CTCGAG	1 cut(s)	505
SfuI	TTCGAA	2 cut(s)	663, 830
SinI	GGWCC	4 cut(s)	599, 1149, 1399, 2147
SlaI	CTCGAG	1 cut(s)	505
SmiMI	CAYNNNNRTG	2 cut(s)	1744, 1984
SmlI	CTYRAG	2 cut(s)	505, 991
SmoI	CTYRAG	2 cut(s)	505, 991
SseBI	AGGCCT	1 cut(s)	2049
SsiI	CCGC	4 cut(s)	125, 728, 1017, 2127
SspI	AATATT	1 cut(s)	514
SspMI	CTAG	5 cut(s)	291, 1250, 1562, 1808, 1931
StuI	AGGCCT	1 cut(s)	2049
StyD4I	CCNGG	4 cut(s)	42, 329, 1685, 1917
StyI	CCWWGG	2 cut(s)	865, 1279
TaaI	ACNGT	8 cut(s)	8, 16, 406, 875, 905, 1069, 1604, 1983
TaiI	ACGT	1 cut(s)	860
TaqI	TCGA	8 cut(s)	472, 506, 663, 830, 1064, 1354, 1435, 1632
TatI	WGTACW	3 cut(s)	905, 1069, 1785
TfiI	GAWTC	5 cut(s)	250, 626, 653, 1136, 1530
Tru1I	TTAA	1 cut(s)	984
Tru9I	TTAA	1 cut(s)	984
TscAI	CASTG	6 cut(s)	451, 695, 1012, 1607, 1986, 2181
TseFI	GTSAC	2 cut(s)	427, 1604
TseI	GCWGC	2 cut(s)	633, 1377
Tsp45I	GTSAC	2 cut(s)	427, 1604
TspDTI	ATGAA	4 cut(s)	287, 444, 645, 1956
TspGWI	ACGGA	1 cut(s)	1515
TspRI	CASTG	6 cut(s)	451, 695, 1012, 1607, 1986, 2181
VpaK11BI	GGWCC	4 cut(s)	599, 1149, 1399, 2147
XapI	RAATTY	8 cut(s)	52, 175, 257, 451, 949, 1165, 1505, 2107
XcmI	CCANNNNNNNNNTGG	1 cut(s)	372
XhoI	CTCGAG	1 cut(s)	505
XmnI	GAANNNNTTC	3 cut(s)	1034, 1623, 2111
XspI	CTAG	5 cut(s)	291, 1250, 1562, 1808, 1931
ZrmI	AGTACT	1 cut(s)	1071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.